Diuretic traditional Chinese medicine fresh medicine composition

Abstract

The invention provides a diuretic traditional Chinese medicine fresh medicine composition, and relates to the technical field of traditional Chinese medicine fresh medicine pharmacy. According to the traditional Chinese medicine theory, the traditional Chinese medicine fresh composition composed of fresh Chinese waxgourd seeds, fresh lotus leaves, fresh dianthus superbus, fresh salvia miltiorrhiza and fresh rhizoma alismatis is obtained based on the principle of dialectical treatment. The fresh traditional Chinese medicine composition has remarkable diuretic and anti-inflammatory effects, the treatment effect is obviously superior to that of a freeze-dried product and a processed product, the effect is equivalent to that of hydrofluthiazide, the fresh traditional Chinese medicine composition can replace western medicines, and meanwhile all the components in the fresh traditional Chinese medicine composition have a synergistic effect and are indispensable. The fresh traditional Chinese medicine composition has no toxic or side effect on mice, and is safe and effective. The fresh traditional Chinese medicine composition provides a new thought for development and application of diuretics.

Description

Technical Field

[0001] The invention relates to the technical field of fresh Chinese medicinal materials for pharmaceutical preparation, and in particular to a fresh Chinese medicinal material composition for diuresis. Background Art

[0002] The urinary system is an important excretory system of the human body. Its main function is excretion. It regulates water and salt metabolism and acid-base balance in the body, produces a variety of biologically active substances, and plays a vital role in maintaining the homeostasis of the body's internal environment. Urinary system diseases mainly include diseases of the kidneys, ureters, bladders, prostatitis, and urethra. Most urinary system diseases are caused by infection, and are also an infectious disease. They are prone to recurrence and difficult to cure. Its clinical manifestations mainly include symptoms such as frequent urination, urgency, pain when urinating, oliguria, polyuria, hematuria, edema, etc. At present, the treatment of urinary system diseases is mainly based on the treatment of the primary disease, combined with diuretics such as diuretics and detumescence, and linshitonglin.

[0003] Diuretics are a commonly used drug in clinical practice. They can directly act on the kidneys, increase the excretion of electrolytes and water, and increase urine volume. The diuretics commonly used in clinical practice are mainly loop diuretics, thiazide diuretics, potassium-sparing diuretics, carbonic anhydrase diuretics, and osmotic diuretics. Although the above diuretics have significant therapeutic effects, the indicators are not fundamental. Long-term use of the above diuretics will produce serious side effects and lead to irreversible organ damage. Traditional Chinese medicine is a life science with Chinese characteristics and a unique health resource in my country. Traditional Chinese medicine has long been aware of the effect of dampness and stranguria, which has been recorded in detail in famous medical works such as "Huangdi Neijing" and "Compendium of Materia Medica". After thousands of years of medical examinations, traditional Chinese medicine diuretics have been widely recognized and used.

[0004] Fresh Chinese medicine refers to fresh plants (including roots, stems, leaves, flowers, fruits, etc.) and fresh animals (including whole animals or organs, tissues, etc.) that have not been dried or processed. They are Chinese medicinal materials that are directly used to treat diseases under the guidance of traditional Chinese medicine theory. Fresh medicine can maintain its natural medicinal smell and pure characteristics because it has not been processed. The application of fresh medicine has a long history. Medicine began when Shennong tasted hundreds of herbs, and medicine at that time generally referred to "fresh medicine". Fresh medicine refers to Chinese medicinal materials that are used fresh after being dug. The active ingredients are fully retained and the drug efficacy is good. Studies have shown that many fresh medicines have a fragrant smell and a light and clear nature. They have the functions of quenching thirst, promoting body fluid, harmonizing the middle and removing dampness, and relieving turbidity and heat. They can treat diseases such as acute syndrome, heat syndrome and superficial syndrome. Their efficacy is very different from that of dried products and processed products. Modern medicine believes that fresh medicine is fresh, unprocessed, and maintains its natural state and molecular structure, so it has a high content of active ingredients and good efficacy. A large amount of modern research data also shows that the chemical composition of fresh medicine is significantly different from that of processed dried medicine, and the content of some active ingredients is often higher. This also reveals the scientific nature of the use of fresh Chinese medicine and the rationality of its clinical use from the perspective of the material basis of the medicine.

[0005] Based on the above research background, the present invention aims to provide a fresh Chinese medicinal composition with definite efficacy, small toxic and side effects, and diuretic and stranguria-relieving effects. Summary of the invention

[0006] 1. Technical issues to be solved

[0007] In view of the deficiencies in the prior art, the present invention provides a fresh Chinese medicinal composition with diuretic and stranguria-relieving effects.

[0008] (II) Technical solution

[0009] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0010] The invention provides a diuretic fresh Chinese medicinal composition. The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 10-25 parts of fresh wax gourd seeds, 10-25 parts of fresh lotus leaves, 5-20 parts of fresh superbus, 5-20 parts of fresh salvia miltiorrhiza, and 5-20 parts of fresh orientalis.

[0011] Furthermore, the fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wax gourd seeds, 10-20 parts of fresh lotus leaves, 5-15 parts of fresh superbus, 5-15 parts of fresh salvia miltiorrhiza, and 5-15 parts of fresh alisma orientalis.

[0012] Furthermore, the fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh superbus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0013] Specifically, in the traditional Chinese medicine fresh medicine composition, fresh wax gourd seeds are seeds of Benincasahispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh superbus is the whole herb of Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh salvia is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh alisma is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alisma family; the above medicinal herbs are freshly picked when used without any processing or preparation.

[0014] When used, the fresh Chinese medicinal composition of the present invention is weighed according to the above weight portions and mashed into juice for oral administration.

[0015] The pharmacological effects of each Chinese herbal medicine in the Chinese herbal medicine fresh medicine composition of the present invention are as follows:

[0016] Winter melon seeds: sweet in taste, cool in nature; have the effects of moistening the lungs, resolving phlegm, eliminating carbuncle, and promoting diuresis; used for phlegm-heat cough, lung abscess, intestinal abscess, gonorrhea, edema, athlete's foot, hemorrhoids, nasal and facial alcohol, etc.

[0017] Lotus leaf: bitter in taste, neutral in nature, enters the liver, spleen, and stomach meridians; has the effects of clearing away heat and dampness, promoting the flow of clear yang, cooling blood and stopping bleeding; used for symptoms such as summer heat and thirst, summer-damp diarrhea, spleen deficiency diarrhea, blood heat vomiting and epistaxis, and bloody stool and metrorrhagia.

[0018] Dianthus superbus: bitter in taste, cold in nature, enters the heart, kidney, small intestine and bladder meridians; has the effects of clearing away heat, promoting diuresis, breaking up blood and dredging menstruation; treats urinary obstruction, gonorrhea, edema, amenorrhea, carbuncle, red eyes and cataracts, and ulcers.

[0019] Salvia miltiorrhiza: It is bitter and slightly cold in nature, and enters the heart, pericardium, and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and relieving pain, relieving restlessness and calming the mind. It is mainly used to treat irregular menstruation, postpartum abdominal pain caused by blood stasis, injuries, irritability and other diseases in women.

[0020] Alisma: sweet, light in taste, cold in nature; enters the kidney and bladder meridians; has the effects of diuresis, heat dissipation, turbidity removal and lipid reduction; used for urinary problems, edema, diarrhea, oliguria, phlegm and fluid dizziness, hot and painful stranguria, and hyperlipidemia.

[0021] (III) Beneficial effects

[0022] Fresh medicine is a summary of the experience of traditional Chinese medicine in using medicine for thousands of years, and is a feature of Chinese medicine diagnosis and treatment, and its clinical application has a long history. Fresh medicine retains the natural activity of the medicine, and has the characteristics of rich active ingredients, fast efficacy and good quality. The present invention complies with the theory of traditional Chinese medicine and takes syndrome differentiation and treatment as the principle to obtain a Chinese medicine fresh medicine composition composed of fresh wax gourd seeds, fresh lotus leaves, fresh superbus, fresh salvia miltiorrhiza and fresh orientalis. In the traditional Chinese medicine fresh medicine composition of the present invention, fresh wax gourd seeds are the main medicine, which mainly realize the effects of clearing away heat and resolving phlegm, promoting diuresis and relieving stranguria, and are the core medicine of the whole prescription; fresh lotus leaves are the ministerial medicine, which assist the main medicine in clearing away summer heat and removing dampness, raising yang and stopping bleeding, and enhancing the effect of the prescription in clearing away heat and removing dampness; fresh Dianthus superbus is the adjuvant, which assists the main and ministerial medicines in clearing away heart heat, promoting damp heat in the small intestine and bladder, and further enhancing the effect of promoting diuresis and relieving stranguria; fresh Salvia miltiorrhiza is the adjuvant, which promotes blood circulation and removes blood stasis, assists the main and ministerial medicines in improving blood circulation, and may also help relieve symptoms caused by damp heat; fresh Alisma orientalis is also the adjuvant, which promotes diuresis and eliminates dampness, and relieves heat, and works together with the main medicine fresh wax gourd seeds to enhance the effect of promoting diuresis and relieving stranguria. The whole prescription plays the effects of promoting diuresis and relieving stranguria, promoting blood circulation and removing blood stasis, and eliminating dampness and relieving heat.

[0023] The fresh Chinese medicine combination of the present invention has significant diuretic and anti-inflammatory effects, and the therapeutic effect is significantly better than that of freeze-dried products and processed products, and is equivalent to the effect of hydroflumethiazide, and can replace Western medicine. At the same time, the various components in the fresh medicine composition of the present invention are synergistic and indispensable. The fresh Chinese medicine combination of the present invention has no toxic side effects on mice, is safe and effective. The fresh Chinese medicine composition of the present invention provides a new idea for the development and application of diuretics. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] A diuretic fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 10 parts of fresh wax gourd seeds, 10 parts of fresh lotus leaves, 5 parts of fresh superbus, 5 parts of fresh salvia miltiorrhiza, and 5 parts of fresh alisma orientalis.

[0027] In the traditional Chinese medicine fresh medicine composition, fresh wax gourd seeds are seeds of Benincasa hispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh superbus is the whole herb of Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh salvia is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh alisma is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alisma family; the above medicinal herbs are freshly picked when used without any processing or preparation.

[0028] Example 2

[0029] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh wax gourd seeds, 25 parts of fresh lotus leaves, 20 parts of fresh superbus, 20 parts of fresh salvia miltiorrhiza, and 20 parts of fresh alisma orientalis.

[0030] Example 3

[0031] The difference between this embodiment and embodiment 1 is that the Chinese medicinal fresh medicine composition is composed of the following raw materials in parts by weight: 14 parts of fresh wax gourd seeds, 12 parts of fresh lotus leaves, 8 parts of fresh superbus, 7 parts of fresh salvia miltiorrhiza, and 6 parts of fresh alisma orientalis.

[0032] Example 4

[0033] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 22 parts of fresh wax gourd seeds, 20 parts of fresh lotus leaves, 16 parts of fresh superbus, 14 parts of fresh salvia miltiorrhiza, and 14 parts of fresh orientalis.

[0034] Example 5

[0035] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 15 parts of fresh wax gourd seeds, 10 parts of fresh lotus leaves, 5 parts of fresh superbus, 5 parts of fresh salvia miltiorrhiza, and 5 parts of fresh orientalis.

[0036] Example 6

[0037] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 25 parts of fresh wax gourd seeds, 20 parts of fresh lotus leaves, 15 parts of fresh superbus, 15 parts of fresh salvia miltiorrhiza, and 15 parts of fresh orientalis.

[0038] Example 7

[0039] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 18 parts of fresh wax gourd seeds, 14 parts of fresh lotus leaves, 10 parts of fresh superbus, 8 parts of fresh salvia miltiorrhiza, and 8 parts of fresh orientalis.

[0040] Example 7

[0041] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 16 parts of fresh lotus leaves, 12 parts of fresh superbus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0042] Example 8

[0043] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh superbus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh orientalis.

[0044] Test example

[0045] Study on the acute toxicity of the diuretic and anti-inflammatory effects of the fresh Chinese medicinal composition of the present invention

[0046] 1 Materials

[0047] 1.1 Experimental drugs

[0048] Test drug 1: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh superbus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh alisma orientalis.

[0049] Test drug 2: 13 portions of fresh Dianthus superbus, 10 portions of fresh Salvia miltiorrhiza, and 10 portions of fresh Alisma orientalis.

[0050] Test drug 3: 20 portions of fresh wax gourd seeds and 18 portions of fresh lotus leaves.

[0051] Test drug 4: freeze-dried drink of test drug 1.

[0052] Test drug 5: 20 parts of wax gourd seeds, 18 parts of lotus leaves, 13 parts of superbus, 10 parts of salvia miltiorrhiza, and 10 parts of oriental rhizome.

[0053] Test drug 6: Hydrochlorothiazide tablets, Changzhou Pharmaceutical Factory Co., Ltd., 25 mg × 100 tablets.

[0054] Among the test drugs 1-3, fresh wax gourd seeds are seeds of Benincasa hispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh superbus is the whole herb of Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh salvia is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; fresh alisma is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alisma genus of the Alismataceae family; the above-mentioned herbs are freshly picked when used without any processing or preparation.

[0055] Preparation of test drugs 1-3: Weigh each fresh medicinal material according to the above-mentioned weight proportions, put it into an ultracentrifugal grinder at 18000rm, then pass it through an 80-mesh sieve, and then use a fruit and vegetable wall breaker to mix, squeeze and crush it, and then use purified water to prepare a 1g / mL suspension to obtain test drugs 1-3, which are stored in a 4°C medicine cool cabinet.

[0056] Preparation of test drug 4: Weigh each fresh Chinese medicinal material according to the above-mentioned weight proportions, pre-freeze at -30°C for more than 24 hours, put into a vacuum freeze dryer, set the time and pressure, set the vacuum degree to 0.2 mbar, set the temperature to -50°C, set the freeze-drying time to 48 hours, and perform freeze-drying; take out and crush, pass through an 80-mesh sieve, and configure into a 1 g / mL suspension with purified water to obtain test drug 4, which is stored in a 4°C medicine cool cabinet.

[0057] Preparation of test drug 5: Weigh each Chinese medicinal material according to the above-mentioned weight proportions (the Chinese medicinal materials used were purchased from Zhang Zhongjing Pharmacy, and all were processed medicines prepared in accordance with the pharmacopoeia methods and standards), wash, dry, soak in 3-4 times purified water for 30 minutes, then boil for 40 minutes, boil twice, combine the filtrate and concentrate to prepare a Chinese medicinal stock solution with a drug concentration of 1 g / mL, to obtain test drug 5.

[0058] 2 Experimental methods

[0059] 2.1 Diuresis experiment

[0060] 2.1.1 Experimental animals

[0061] Take SPF grade SD rats, male, weighing 180-220g. The animals are raised in an SPF laboratory, and after being grouped into cages according to the random number table method, the feeding conditions are maintained: temperature (26±2)℃, relative humidity (49±5)%, feeding on time every day, and unlimited water supply. The Aston method is used to screen SD rats: first, the rats are adaptively fed in metabolic cages for 3 days. After the adaptive feeding is completed, they are fasted but not watered for 18 hours, and gavage with deionized water at a volume of 25mL / kg. The urine of the rats two hours after the gavage is collected, and the rats with urine mass greater than 40% of the gavage mass are screened as qualified rats for this diuresis experiment.

[0062] 2.1.2 Experimental groups and drug administration

[0063] 84 qualified rats were selected and randomly divided into a blank control group (blank group), a positive control group (hydrochlorothiazide group), and experimental groups 1-5 according to the weight range, with 12 rats in each group. The experiment adopted the metabolic cage method, and each group was gavaged according to the prescribed dosage for 7 consecutive days. The administration method is shown in Table 1.

[0064] Table 1 Drug administration methods for rats in each group

[0065] Grouping Quantity(n) drug Dosage Blank group 12 Pure water Same volume Hydrochlorothiazide group 12 Hydrochlorothiazide Tablets 16mg / kg aqueous solution Experimental Group 1 12 Test drug 1 10g / kg Experimental Group 2 12 Test drug 2 10g / kg Experimental Group 3 12 Test drug 3 10g / kg Experimental Group 4 12 Test drug 4 3g / kg Experimental Group 5 12 Test drug 5 3g / kg

[0066] The drug was administered continuously for 7 days. The rats were fasted but not watered for 24 hours before the last administration. Each group was gavaged with 0.9% sodium chloride solution at a dose of 25 mL / kg (which can increase the extracellular fluid of the animals and simulate the state of water and sodium retention). 20 minutes later, the drug was administered by gavage according to the dose, and the blank group was given the same volume of normal saline. After administration, the lower abdomen of the rat was gently pressed to empty the urine in the bladder, and the rat was placed in a metabolic cage. Urine was collected once every hour for a total of 5 times, and the urine volume per hour was recorded. The last urine collection required emptying the urine in the rat's bladder. The collected urine was used to detect Na + , K + , Cl — The amount of ions discharged.

[0067] 2.1.3 Detection indicators and methods

[0068] (1) Collect and record the urine volume of rats in each group within 5 hours after the last administration.

[0069] (2) Determine the excretion of Na+, K+, and Cl- ions in urine.

[0070] 2.2 Carrageenan-induced swelling of rat toes

[0071] A total of 70 SPF male SD rats were randomly divided into 7 groups according to body weight, with 12 rats in each group. The grouping and administration methods were as shown in 2.1.2. Each group was administered by gavage once a day for 7 consecutive days. 60 minutes after the last administration, 0.1 mL of 1% carrageenan was subcutaneously injected into the right hind toe of each rat. The volume of the right hind toe of each rat was measured 1, 3, and 6 hours after injection, and the swelling degree of the right hind toe and the toe swelling inhibition rate of each group of rats at different times after inflammation were compared.

[0072] Toe swelling Δm = toe volume after inflammation - toe volume before inflammation

[0073] Toe swelling inhibition rate (I%) = (average toe swelling degree of model group - average toe swelling degree of drug administration group) / average toe swelling degree of model group × 100%.

[0074] 2.3 Acute toxicity test

[0075] 2.3.1 Half-lethal experiment

[0076] 40 Kunming mice weighing (20±2)g were selected and randomly divided into 4 groups, 10 mice in each group, half male and half female. Group 1 was the high-dose group (40g / kg) of test drug 1, group 2 was the medium-dose group (20g / kg) of test drug 1, group 3 was the low-dose group (10g / kg) of test drug 1, and group 4 was the control group. The mice were fasted but not watered for 1 day before the test. On the second day, the test group mice were gavaged with 0.2mL, and the control group mice were gavaged with an equal volume of saline. They were fed 6 hours after gavage and observed for 7 consecutive days. The death and median lethal dose were recorded. If all the mice in the test drug group survived and the median lethal dose value could not be measured, it means that the test drug has very low toxicity, and the maximum dosage test can be carried out in accordance with the Technical Guidelines for Acute Toxicity Research of Traditional Chinese Medicine and Natural Medicine.

[0077] 2.3.2 Maximum dosage experiment

[0078] Twenty Kunming mice weighing (20±2)g were selected and randomly divided into two groups, 10 mice in each group, half male and half female. The experimental group was gavaged with the maximum dose (40g / kg) and the maximum gavage volume (40mL / kg) of the test drug 1, and the control group was gavaged with an equal volume of normal saline. After administration, the mice were observed for 7 consecutive days, and the clinical manifestations, weight changes and deaths of the mice were observed. After gavage, the mice were observed for 7 consecutive days, and the clinical manifestations of the mice were recorded. At the same time, the weight of the mice was measured on the 1st, 3rd, 5th and 7th day of gavage, and the death was observed.

[0079] 2.4 Statistical analysis

[0080] SPSS 19.0 statistical software was used for analysis, and the experimental data were expressed as mean (Meat) ± standard deviation (SD). The comparison between two groups was performed by single-sample t test, and the comparison between multiple groups was performed by Homogeneity of variance test in one-way ANOVO. P < 0.05 indicated that the difference was statistically significant, and P < 0.01 indicated that the difference was statistically significant. GraphPrism 8.0.1 software was used for drawing.

[0081] 3 Results

[0082] 3.1 Effect of the fresh Chinese medicinal composition of the present invention on urine volume in water-loaded rat model

[0083] As shown in Table 2, 1 hour after administration, compared with the blank group, the urine volume of rats in the hydrochlorothiazide group and experimental groups 1-5 increased significantly, and the difference was statistically significant (p<0.05, p<0.01); the urine volume of rats in experimental group 1 was significantly higher than that in experimental groups 2-3 and 5, and the difference was significant (p<0.05, p<0.01), and there was no statistically significant difference compared with the hydrofluthiazide group and experimental group 4 (p>0.05). 3 hours and 5 hours after administration, compared with the blank group, the urine volume of rats in the hydrochlorothiazide group and experimental groups 1-5 increased significantly, and the difference was statistically significant (p<0.05, p<0.01); the urine volume of rats in experimental group 1 was significantly higher than that in experimental groups 2-5, and the difference was significant (p<0.05, p<0.01), and there was no statistically significant difference compared with the hydrofluthiazide group (p>0.05). The above results show that the fresh Chinese medicinal composition (test drug 1) of the present invention has a significant diuretic effect, which is equivalent to the diuretic effect of the Western medicine hydrochlorothiazide.

[0084] Table 2 Urine volume of water-loaded rat models in each group n=12)

[0085] Group 1h(mL) 3h(mL) 5h(mL) Blank group <![CDATA[1.39±0.22 ## ]]> <![CDATA[3.48±0.34 ## ]]> <![CDATA[5.01±0.27 ## ]]> Hydrochlorothiazide group 2.31±0.43** 6.82±0.39** 9.06±0.41** Experimental Group 1 2.39±0.37** 6.88±0.33** 9.67±0.32** Experimental Group 2 <![CDATA[1.78±0.30* ## ]]> <![CDATA[4.01±0.30* ## ]]> <![CDATA[6.83±0.33** ## ]]> Experimental Group 3 <![CDATA[1.88±0.31** ## ]]> <![CDATA[4.16±0.40** ## ]]> <![CDATA[6.21±0.20** ## ]]> Experimental Group 4 2.20±0.29** <![CDATA[5.59±0.34** ## ]]> <![CDATA[7.62±0.41** ## ]]> Experimental Group 5 <![CDATA[2.03±0.29** # ]]> <![CDATA[5.51±0.38** ## ]]> <![CDATA[7.52±0.34** ## ]]>

[0086] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0087] 3.2 Effect of the fresh Chinese medicinal composition of the present invention on the sodium ions in urine of rats with water loading within 5 h + , K + , Cl － The impact of discharge

[0088] As shown in Table 3, compared with the blank group, the hydrochlorothiazide group and experimental groups 1, 4 and 5 were able to significantly increase Na + , K + , Cl － The difference was statistically significant (p<0.05, p<0.01). + , K + , Cl － The excretion of Na in the hydrochlorothiazide group was higher than that in the experimental groups 2-5. + , K + , Cl － The results show that the Chinese herbal medicine fresh drug composition (test drug 1) of the present invention has more advantages in promoting the excretion of electrolytes in urine, and has a diuretic effect comparable to that of the Western medicine hydrochlorothiazide.

[0089] Table 3 Na in urine within 5 h in each group of water-loaded rat models + , K + , Cl － Discharge n=12)

[0090] Group <![CDATA[Na + ( mmol / L )]]> <![CDATA[K + ( mmol / L )]]> <![CDATA[Cl － ( mmol / L )]]> Blank group <![CDATA[126.37±8.02 ## ]]> <![CDATA[3.06±1.36 # ]]> <![CDATA[127.88±8.06 ## ]]> Hydrochlorothiazide group 153.32±13.31** 15.99±2.94* 145.77±14.29** Experimental Group 1 151.57±7.79** 16.63±1.64* 142.87±12.78** Experimental Group 2 <![CDATA[130.59±14.94 ## ]]> 9.36±3.16 134.78±11.41 Experimental Group 3 <![CDATA[131.49±18.21 ## ]]> 10.44±2.09 <![CDATA[132.69±10.24 # ]]> Experimental Group 4 145.82±19.96** 14.68±3.11* 139.88±11.38* Experimental Group 5 <![CDATA[139.91±12.79* # ]]> 14.35±2.48* 138.99±10.13*

[0091] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0092] 3.3 Effect of the fresh Chinese medicinal composition of the present invention on carrageenan-induced swelling of rat toes

[0093] As shown in Table 4, compared with the blank group, hydrochlorothiazide and test drugs 1-5 had a significant inhibitory effect on carrageenan-induced rat toe swelling, and the difference was statistically significant (p<0.01). The inhibitory effect of the Chinese herbal medicine fresh drug composition of the present invention on carrageenan-induced rat toe swelling was significantly higher than that of test drugs 2-6 (p<0.01), and the results showed that the Chinese herbal medicine fresh drug composition of the present invention had a good anti-inflammatory effect.

[0094] Table 4 Carrageenan-induced swelling of rat toes n=12)

[0095]

[0096] Note: Compared with the blank group, *p<0.05, **p<0.01; compared with the experimental group 1, # p<0.05, ## p<0.01.

[0097] Table 5 Inhibition rate of the fresh Chinese medicinal composition of the present invention on rat toe swelling caused by carrageenan

[0098]

[0099]

[0100] 3.4 Application of the fresh Chinese medicinal combination of the present invention in the acute toxicity test in mice

[0101] 3.4.1 Half-lethal test

[0102] As shown in Table 6, the mice were observed for 7 days after administration, and all mice in each group showed no abnormalities, good mental state, and no death was found. No pathological changes were found in the organ tissues after autopsy.

[0103] Table 6 Death of mice

[0104] Group quantity Deaths Survival rate (%) Control group 10 0 100 Low dose group 10 0 100 Medium dose group 10 0 100 High dose group 10 0 100

[0105] 3.4.2 Maximum dosage experiment

[0106] As shown in Table 7, the mice were observed for 7 days after administration, and all mice in each group showed no abnormalities, good mental state, and no death was found. No pathological changes were found in the organ tissues after autopsy.

[0107] As shown in Table 8, the body weight of mice in each group increased normally during the experiment, with no significant difference (P>0.05).

[0108] Table 7 Death of mice

[0109] Group quantity Deaths Survival rate (%) Control group 10 0 100 Experimental Group 10 0 100

[0110] Table 8 Changes in mouse body weight

[0111] Group 1d 3d 5d 7d Control group 21.42±2.44 23.86±0.67 24.11±0.25 24.63±0.41 Experimental Group 21.37±1.58 23.42±0.79 23.99±0.57 24.38±0.64

[0112] 4 Conclusion

[0113] Fresh medicine is a summary of the experience of using medicine in traditional Chinese medicine for thousands of years, and is a feature of Chinese medicine diagnosis and treatment, and its clinical application has a long history. Fresh medicine retains the natural activity of medicine, has the characteristics of rich active ingredient quantity, fast curative effect and good quality. The present invention complies with the theory of traditional Chinese medicine, takes syndrome differentiation and treatment as the principle, and obtains the Chinese medicine fresh medicine composition composed of fresh wax gourd seeds, fresh lotus leaves, fresh Dianthus superbus, fresh Salvia miltiorrhiza and fresh Rhizoma Alismatis. According to the above experimental results, the Chinese medicine fresh medicine combination of the present invention has significant diuretic and anti-inflammatory effects, and the effect is significantly better than the lyophilized product (test drug 4) and the processed product (test drug 5), which is equivalent to the effect of hydroflumethiazide, and can replace Western medicine. At the same time, each component in the fresh medicine composition of the present invention is synergistic, and none of them is indispensable. The Chinese medicine fresh medicine combination of the present invention has no toxic side effects on mice, and is safe and effective. The Chinese medicine fresh medicine composition of the present invention provides a new idea for the development and application of diuretics.

[0114] In the traditional Chinese medicine fresh medicine composition of the present invention, fresh wax gourd seeds are the main medicine, which mainly realize the effects of clearing away heat and resolving phlegm, promoting diuresis and relieving stranguria, and are the core medicine of the whole prescription; fresh lotus leaves are the ministerial medicine, which assist the main medicine in clearing away summer heat and removing dampness, raising yang and stopping bleeding, and enhancing the effect of the prescription in clearing away heat and removing dampness; fresh Dianthus superbus is the adjuvant, which assists the main and ministerial medicines in clearing away heart heat, promoting damp heat in the small intestine and bladder, and further enhancing the effect of promoting diuresis and relieving stranguria; fresh Salvia miltiorrhiza is the adjuvant, which promotes blood circulation and removes blood stasis, assists the main and ministerial medicines in improving blood circulation, and may also help relieve symptoms caused by damp heat; fresh Alisma orientalis is also the adjuvant, which promotes diuresis and eliminates dampness, and relieves heat, and works together with the main medicine fresh wax gourd seeds to enhance the effect of promoting diuresis and relieving stranguria. The whole prescription plays the effects of promoting diuresis and relieving stranguria, promoting blood circulation and removing blood stasis, and eliminating dampness and relieving heat.

[0115] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features thereof may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A diuretic Chinese medicinal fresh medicine composition, characterized in that: The traditional Chinese medicine fresh medicine composition is composed of the following raw materials in parts by weight: 10-25 parts of fresh wax gourd seeds, 10-25 parts of fresh lotus leaves, 5-20 parts of fresh superbus, 5-20 parts of fresh salvia miltiorrhiza, and 5-20 parts of fresh orientalis.

2. A diuretic Chinese medicinal fresh medicine composition according to claim 1, characterized in that: The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wax gourd seeds, 10-20 parts of fresh lotus leaves, 5-15 parts of fresh superbus, 5-15 parts of fresh salvia miltiorrhiza, and 5-15 parts of fresh orientalis.

3. A diuretic Chinese medicinal fresh medicine composition according to claim 1, characterized in that: The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 20 parts of fresh wax gourd seeds, 18 parts of fresh lotus leaves, 13 parts of fresh superbus, 10 parts of fresh salvia miltiorrhiza, and 10 parts of fresh orientalis.

4. A diuretic Chinese medicinal fresh medicine composition according to any one of claims 1 to 3, characterized in that: In the traditional Chinese medicine fresh medicine composition, fresh wax gourd seeds are seeds of Benincasa hispida (Thunb.) Cogn., a plant of the Cucurbitaceae family; fresh lotus leaves are leaves of Nelumbo nucifera Gaertn, a plant of the Nymphaeaceae family; fresh superbus is the whole herb of Dianthus superbus L., a perennial herb of the Caryophyllaceae family; fresh salvia is the root of Salvia miltiorrhiza Bunge, a perennial upright herb of the Lamiaceae family; and fresh alisma is the stem of Alisma plantago-aquatica L., a perennial aquatic or marsh herb of the Alisma family; the above medicinal herbs are freshly picked when used without any processing or preparation.

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