Kidney-tonifying and blood-activating formula for preventing and treating osteoporosis as well as preparation method and application thereof

Abstract

The invention discloses a kidney-tonifying and blood-activating formula for preventing and treating osteoporosis as well as a preparation method and application of the kidney-tonifying and blood-activating formula. The kidney-tonifying and blood-activating formula is prepared from radix aconiti lateralis preparata, radix rehmanniae preparata, radix morindae officinalis, rhizoma curculiginis, radix salviae miltiorrhizae and radix glycyrrhizae. Monkshood can tonify kidney to strengthen yang and eliminate cold to stop pain; radix rehmanniae preparata has the effects of replenishing blood, nourishing yin, boosting essence and filling marrow and is used as monarch drugs; salvia miltiorrhiza can promote blood circulation to remove blood stasis, calm nerves and eliminate The morinda officinalis tonifies the kidney, strengthens tendons, dispels wind and Curculigo orchioides can warm kidney, strengthen tendons, dispel cold and remove dampness as ministerial drugs; the liquorice harmonizes the medicines as a guide; the whole formula is precise and appropriate in compatibility, simple in medicine and good in effect, and has the effects of tonifying kidney, nourishing yin, boosting essence and filling marrow. The kidney-tonifying and blood-activating formula is a pure traditional Chinese medicine formula, is reasonable in compatibility, conforms to the theoretical basis of traditional Chinese medicine and a dialectical analysis method, is high in safety and exact in curative effect, and provides a new treatment thought for osteoporosis.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis, and a preparation method and use thereof. Background Art

[0002] Osteoporosis (OP) is a systemic bone disease characterized by low bone mass and damaged bone microstructure, which leads to increased bone fragility and susceptibility to fractures. Clinically, it mainly manifests as fatigue, pain in the back or limbs, spinal deformity and even fractures. Most patients can have their symptoms alleviated or relieved after active and effective treatment. If older patients cannot fully recover, fragility fractures may be accompanied by chronic pain and disability, and in severe cases, death.

[0003] Osteoporosis is a multi-etiological disease, and its basic pathological mechanism is the defect in the coupling of bone resorption and bone formation during bone metabolism. It is known that various hormones in the systemic circulation, some local regulatory factors in bone metabolism, physical exercise, physical factors and nutritional factors may affect the process of bone metabolism and bone remodeling. However, among the many links, which factors play a role and how the factors interact with each other need to be further revealed.

[0004] Traditional Chinese medicine believes that the occurrence of osteoporosis is mainly related to three factors: kidney deficiency, spleen deficiency and blood stasis.

[0005] There are three main treatment modes for osteoporosis: Western medicine, Chinese medicine and integrated Chinese and Western medicine. Most of the existing treatment drugs can only relieve pain, but the bone density change is not obvious, which can easily cause the recurrence of osteoporosis. Summary of the invention

[0006] The object of the present invention is to provide a kidney-tonifying and blood-activating prescription for preventing or treating osteoporosis and its preparation method and use according to the deficiencies of the prior art. The kidney-tonifying and blood-activating prescription of the present invention has a precise compatibility, simple medicine and great efficacy, has the effects of tonifying the kidney and nourishing yin, replenishing essence and filling marrow, and has a significant effect on the treatment of osteoporosis.

[0007] The invention provides a kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis. The kidney-tonifying and blood-activating prescription is prepared from cooked aconite root, cooked rehmannia root, Morinda officinalis, curculigo orchioides, salvia miltiorrhiza and liquorice.

[0008] Specifically, the kidney-tonifying and blood-activating prescription comprises the following raw materials in parts by weight:

[0009] 5-15 parts of cooked aconite root, 15-25 parts of cooked rehmannia root, 10-20 parts of Morinda officinalis, 5-15 parts of Curculigo orchioides, 15-25 parts of Salvia miltiorrhiza and 4-8 parts of Licorice.

[0010] In one embodiment, the kidney-tonifying and blood-activating prescription comprises the following ingredients in parts by weight:

[0011] 10 parts of Radix Aconiti Lateralis Preparata, 20 parts of Radix Rehmanniae Preparata, 15 parts of Radix Morindae Officinalis, 10 parts of Curculigo orchioides, 20 parts of Radix Salviae Miltiorrhizae and 6 parts of Radix Glycyrrhizae.

[0012] The preparation method of the invigorating kidney and activating blood circulation prescription for preventing and treating osteoporosis is as follows: weigh various medicinal materials, add 6-8 times of water and boil twice, each time for 0.5-1 hour, combine the two decoctions, concentrate to an extract with a relative density of 0.05-1.15, spray dry, and pass the dried fine powder through a 14-16 mesh sieve to obtain the extract.

[0013] Furthermore, the preparation method of the Chinese medicine composition for preventing or treating the invigorating kidney and activating blood circulation of the present invention is as follows: weigh each medicinal material, add 8 times the amount of water and decoct twice, each time for 1 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.15, spray dry, and pass the dried fine powder through a 16-mesh sieve to obtain.

[0014] Furthermore, the preparation of the kidney-tonifying and blood-activating prescription is a decoction, a pill, a gel, a tablet, a mixture, a capsule, a granule, a powder or a paste.

[0015] The present invention provides use of the kidney-tonifying and blood-activating prescription in preparing a medicine for preventing or treating osteoporosis.

[0016] According to TCM syndrome differentiation, osteoporosis belongs to the category of "bone atrophy" and "bone arthritis". "Kidney is the foundation of innate constitution" and "kidney governs bones". It is believed that the growth and development of bones, whether strong or not, is closely related to the rise and fall of kidney essence. When kidney essence is abundant, bones grow actively, and bones are strong; when kidney essence is weak, bones are not nourished, tendons and bones are atrophied. Blood stasis is also an important factor in causing osteoporosis. Blood stasis can often be caused by spleen and kidney dysfunction, and the generation of blood stasis will further aggravate spleen and kidney damage, thereby aggravating the progression of osteoporosis. Therefore, according to TCM theory, osteoporosis is mainly caused by kidney deficiency and essence deficiency. Kidney deficiency and kidney essence deficiency, spleen dysfunction, bone malnutrition, resulting in fragile and weak bones, back pain, shin and knee soreness, etc.

[0017] The invention starts from the pathogenesis, studies traditional Chinese medicines, combines dialectical argumentation, and obtains a kidney-tonifying and blood-activating prescription composed of cooked aconite root, cooked rehmannia root, Morinda officinalis, curculigo orchioides, salvia miltiorrhiza and liquorice.

[0018] Cooked Aconite: Pungent, warm, has the effects of dispelling cold and relieving pain, restoring yang and rescuing adverse conditions, replenishing fire and supporting yang. It regulates adrenal axis dysfunction, has an enhanced estrogen-like effect, and resists myocardial ischemia and hypoxia.

[0019] Rehmannia glutinosa: sweet, slightly warm, enters the liver and kidney meridians, nourishes blood and yin, replenishes essence and fills marrow. Used for blood deficiency, palpitations, irregular menstruation, metrorrhagia, liver and kidney yin deficiency, soreness of waist and knees, bone steaming and hot flashes, night sweats and spermatorrhea, internal heat and thirst, dizziness, tinnitus, and premature graying of hair.

[0020] Morinda officinalis: pungent, sweet, slightly warm, enters the liver and kidney meridians, nourishes the kidney and strengthens yang, strengthens tendons and bones, dispels wind and dampness. It is mainly used to treat wind-cold-dampness arthralgia, soreness of waist and knees, rheumatism and beriberi, etc. Pharmacologically, it has strengthening, anti-fatigue, anti-depression and anti-tumor effects.

[0021] Curculigo: pungent, warm, enters the kidney and liver meridians, warms the kidney yang, strengthens the tendons and bones, treats impotence, cold semen, urinary incontinence, metrorrhagia, cold pain in the heart and abdomen, cold numbness of the waist and feet, carbuncle, scrofula, and cold diarrhea due to yang deficiency.

[0022] Danshen: bitter, slightly cold, enters the heart and liver meridians, activates blood circulation and removes blood stasis, relieves pain, clears the heart and relieves vexation, cools blood and eliminates carbuncle. Existing studies have shown that Danshen can increase the formation of collagen fibers and rich calcium salt deposition during fractures, proving that Danshen can mobilize calcium from adjacent fractured bone tissue to meet the calcium needs of new bone formation.

[0023] Licorice: sweet, neutral, enters the spleen, stomach, heart, and lung meridians, and can relieve urgency, moisten the lungs, detoxify, and harmonize various medicines. According to the "Ben Jing", it can treat cold and heat evil spirits in the five internal organs, strengthen tendons and bones, grow muscles, double strength, treat wounds and swellings, and detoxify.

[0024] Pharmacodynamic studies have shown that the invigorating kidney and activating blood circulation prescription of the present invention increases the serum calcitonin level of castrated osteoporotic rats, increases bone density, improves bone quality, and significantly improves the symptoms of osteoporosis, thereby achieving the effect of treating osteoporosis.

[0025] Compared with the prior art, the present invention has the following advantages:

[0026] The kidney-tonifying and blood-activating prescription of the present invention is prepared from cooked aconite, cooked rehmannia, Morinda officinalis, Curculigo orchioides, Salvia miltiorrhiza and liquorice. In the prescription, aconite tonifies the kidney and strengthens yang, dispels cold and relieves pain; cooked rehmannia tonifies blood and nourishes yin, replenishes essence and fills the marrow, and is the main medicine; Salvia miltiorrhiza promotes blood circulation and removes blood stasis, calms the nerves and eliminates carbuncle; Morinda officinalis tonifies the kidney and strengthens the tendons, dispels wind and removes dampness; Curculigo orchioides warms the kidney and strengthens the tendons, dispels cold and removes dampness, and is the minister medicine. Licorice is used to harmonize the various medicines. The whole prescription is precisely matched, the medicine is simple and the effect is great, and it has the effects of tonifying the kidney and nourishing yin, replenishing essence and filling the marrow. The kidney-tonifying and blood-activating prescription of the present invention is a pure Chinese medicine prescription, with reasonable matching, in line with the theoretical basis of traditional Chinese medicine and the dialectical analysis method, high safety, and definite efficacy, providing a new treatment idea for osteoporosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Comparison of calcitonin levels in each group

[0028] Compared with the model control group, * P<0.05, ** P<0.01; compared with Example 1 group, # P<0.05, ## P<0.01.

[0029] Figure 2 Comparison of bone density among groups

[0030] Compared with the model control group, * P<0.05, ** P<0.01; compared with Example 1 group, # P<0.05, ## P<0.01.

[0031] Figure 3 To compare the clinical efficacy between the two groups. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further described in detail below in conjunction with the accompanying drawings of the specification, but the protection scope of the present invention is not limited to the following.

[0033] Example 1

[0034] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0035] Cooked Aconite 10g, Cooked Rehmannia 20g, Morinda officinalis 15g, Curculigo orchioides 10g, Salvia miltiorrhiza 20g and Licorice 6g.

[0036] Take the above-mentioned medicinal materials, add 8 times the amount of water and boil twice, each time for 1 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.15, add starch slurry to make a soft material, sieve through a 16-mesh sieve and then dry, add an appropriate amount of magnesium stearate and press into tablets to obtain the kidney-tonifying and blood-activating prescription tablets.

[0037] Example 2

[0038] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0039] Cooked Aconite 15g, Cooked Rehmannia 15g, Morinda officinalis 20g, Curculigo orchioides 5g, Salvia miltiorrhiza 25g and Licorice 4g.

[0040] Take the above-mentioned medicinal materials, add 6 times the amount of water and boil twice, each time for 0.5 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.05, add starch slurry to make a soft material, sieve through a 16-mesh sieve and then dry, add an appropriate amount of magnesium stearate and package into bags to obtain the granules of the Bushen Huoxue prescription.

[0041] Example 3

[0042] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0043] Cooked Aconite 5g, Cooked Rehmannia 25g, Morinda officinalis 10g, Curculigo orchioides 15g, Salvia miltiorrhiza 15g and Licorice 8g.

[0044] Take the above-mentioned medicinal materials, add 8 times the amount of water and boil twice, each time for 0.5 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.05, add starch slurry to make a soft material, sieve through a 16-mesh sieve and then dry, add an appropriate amount of magnesium stearate and press into tablets to obtain the kidney-tonifying and blood-activating prescription tablets.

[0045] Example 4

[0046] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0047] Cooked Aconite 10g, Cooked Rehmannia 15g, Morinda officinalis 20g, Curculigo orchioides 10g, Salvia miltiorrhiza 20g and Licorice 6g.

[0048] Take the above-mentioned medicinal materials, add 8 times the amount of water and boil twice, each time for 0.5 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.15, add starch slurry to make a soft material, sieve through a 14-mesh sieve and then dry, add an appropriate amount of magnesium stearate and put into a gelatin capsule shell to obtain the Bushen Huoxue prescription capsules.

[0049] Example 5

[0050] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0051] Cooked Aconite 15g, Cooked Rehmannia 25g, Morinda officinalis 10g, Curculigo orchioides 10g, Salvia miltiorrhiza 15g and Licorice 4g.

[0052] Take the above-mentioned medicinal materials, add 6 times the amount of water and boil twice, each time for 1 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.15, add starch slurry to make a soft material, sieve through a 14-mesh sieve and then dry, add an appropriate amount of magnesium stearate and press into tablets to obtain the kidney-tonifying and blood-activating prescription tablets.

[0053] Comparative Example 1

[0054] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0055] 10g of cinnamon twig, 20g of astragalus, 15g of Morinda officinalis, 10g of curculigo, 20g of salvia miltiorrhiza and 6g of liquorice.

[0056] The preparation method is the same as Example 1.

[0057] Comparative Example 2

[0058] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0059] Cooked Aconite 10g, Cooked Rehmannia Glutinosa 20g, Epimedium and Morinda Officinalis 15g, Curculigo 10g, Angelica 20g and Licorice 6g.

[0060] The preparation method is the same as Example 1.

[0061] Comparative Example 3

[0062] A Chinese medicine composition for preventing or treating osteoporosis, comprising the following raw materials in parts by weight:

[0063] Cooked Aconite 15g, Cooked Rehmannia 10g, Morinda officinalis 10g, Curculigo orchioides 20g, Salvia miltiorrhiza 10g and Licorice 6g.

[0064] The preparation method is the same as Example 1.

[0065] Efficacy Example 1 Experimental study on the effect of the invigorating kidney and activating blood circulation prescription of the present invention on osteoporosis in castrated patients

[0066] 1. Establishment of Osteoporosis Rat Model

[0067] 56 SD rats, SPF grade, female, weighing (200±20) g, were used. They were given free access to water and food. The room temperature was maintained at 20-25°C.

[0068] After 1 week of adaptive feeding of rats, 8 rats were selected as blank control group, and the rest of the rats were modeled. The modeling method was as follows: anesthetized by intraperitoneal injection of 2% sodium pentobarbital, 2 cm longitudinal incisions were made on both sides of the back spine under aseptic conditions, and after muscle separation, yellowish fat masses were visible. The fat masses were pulled out with tweezers, a thread was passed through the lower end of the ovary, ligated, the ovary and the remaining thread were removed, the tissue was pushed back into the abdominal cavity, and the incision was sutured (sham operation was performed in the control group). All animals were routinely raised, and half of the animals were randomly selected after 16 weeks to measure bone density and body weight to determine the modeling situation.

[0069] The modeled rats were randomly divided into 6 groups according to their body weight, with 8 rats in each group, namely, model control group, Example 1 group, Example 2 group, comparative example 1 group, comparative example 2 group, and comparative example 3 group.

[0070] The medications for each group are as follows:

[0071] The Example 1 group was intragastrically administered with 1.0 g / kg of the Example 1 tablets.

[0072] The Example 2 group was intragastrically administered with 1.0 g / kg of the Example 2 granules.

[0073] The comparative example 1 group was intragastrically administered with 1.0 g / kg comparative example 1 tablets.

[0074] The comparative example 2 group was intragastrically administered with 1.0 g / kg comparative example 2 tablets.

[0075] The comparative example 3 group was intragastrically administered with 1.0 g / kg comparative example 3 tablets.

[0076] Rats in the blank control group and the model control group were gavaged with an equal volume of saline at a dose of 10 mL / kg, once a day for 30 consecutive days.

[0077] 2. Model observation and indicator detection

[0078] One hour after the last administration, the bone density of all animals was measured (dual-energy X-ray absorptiometry), and blood was taken to determine the calcitonin content.

[0079] 3. Statistical methods

[0080] Graphpad Prism 9.0 software was used for data processing, and the measured data were expressed as mean ± standard deviation. One-way analysis of variance was used for comparison among multiple groups, and SNK-q was used for pairwise comparison among groups. The difference was considered statistically significant when P < 0.05.

[0081] 4. Experimental Results

[0082] 4.1 Effects on serum calcitonin levels

[0083] Compared with the model control group, Example 1 group and Example 2 group significantly increased the serum calcitonin level of rats.

[0084] Compared with the comparative examples 1-3, the example 1 group showed significant difference in improving the serum calcitonin level. Figure 1 .

[0085] 4.2 Effects on bone density

[0086] Compared with the model control group, the bone density of rats in Example 1 and Example 2 groups increased, with significant differences.

[0087] Compared with the comparative examples 1-3, the Example 1 group had a significant difference in increasing bone density. Figure 2 .

[0088] The invigorating kidney and activating blood circulation prescription of the present invention increases the serum calcitonin level of castrated osteoporotic rats, increases bone density, significantly improves the symptoms of osteoporosis, and achieves the effect of treating osteoporosis.

[0089] Clinical example: Observation on the therapeutic effect of the Bushen Huoxue prescription of the present invention on osteoporosis

[0090] 1. Materials and Methods

[0091] 1. Clinical data

[0092] The 76 osteoporosis patients who entered this study were randomly divided into a control group and an experimental group of 38 people each. The control group had 20 males and 18 females with an average age of (68.92±7.01) years old; the observation group had 18 males and 20 females with an average age of (67.83±6.57) years old. The age and gender of the two groups were comparable.

[0093] 2. Inclusion criteria: Meet the diagnostic criteria for osteoporosis in the "Guidelines for the Diagnosis and Treatment of Osteoporosis in China"; have no other major diseases; age ≥ 60 years; tolerate Chinese medicine and insist on taking Chinese medicine; voluntarily participate in this study and sign the informed consent.

[0094] 3. Exclusion criteria

[0095] Suffering from serious diseases and being in critical condition; hematopoietic system diseases; taking other drugs that affect bone metabolism; allergic to traditional Chinese medicine.

[0096] 4. Treatment Methods

[0097] Control group: Calcium carbonate D3 chewable tablets (Calcitrate, Wyeth Pharmaceuticals, National Medicine Approval No. H10950029), each tablet contains 300 mg of calcium, 2 tablets / time, once a day, orally; Calcitriol capsules (Rogaiquan, Shanghai Roche Pharmaceuticals Co., Ltd., National Medicine Approval No. J20150011), 0.25 g / capsule, 1 capsule / time, twice a day, orally.

[0098] BSHXD group: Calcium carbonate D3 chewable tablets (Calcitrate, Wyeth Pharmaceuticals, National Medicine Standard No. H10950029), each tablet contains 300 mg of calcium, 2 tablets / time, once a day, orally; BSHXD (10 g of cooked aconite, 20 g of cooked rehmannia, 15 g of Morinda officinalis, 10 g of Curculigo, 20 g of Salvia miltiorrhiza, 6 g of Licorice. The drugs were provided by the pharmacy of the Affiliated Hospital of Shandong University of Traditional Chinese Medicine) was boiled in water to 300-400 ml, and taken once in the morning and evening after meals.

[0099] One course of treatment is 4 weeks, for a total of 3 courses, 12 weeks.

[0100] During the trial medication period, it is not allowed to use other drugs or methods for the treatment of osteoporosis, and the lifestyle and exercise habits are not changed.

[0101] 5.Indicator observation

[0102] (1) Clinical efficacy evaluation criteria: markedly effective means that the symptoms of low back pain and fatigue are greatly alleviated, and X-ray examination shows an increase in bone density; effective means that the symptoms of low back pain and fatigue are alleviated, and X-ray examination shows no significant increase in bone density; ineffective means that the symptoms of low back pain and fatigue are not improved, and X-ray examination shows a decrease in bone density.

[0103] (2) Bone density index: Bone density is measured using a dual-energy X-ray linear scanner to measure the lumbar spine L 1－4 and left femoral neck bone density, reference interval: T-score>-1.

[0104] 6. Statistical methods

[0105] SPSS 22.0 software was used for data processing, and the data were expressed as mean ± standard deviation. The data of each group were subjected to normal distribution test and variance homogeneity test, and the inter-group comparison was performed with one-way analysis of variance, with P < 0.05 as the difference being statistically significant.

[0106] 2. Results

[0107] 1. Comparison of clinical efficacy

[0108] The total effective rate of the BSHXD group of the present invention is better than that of the control group. Figure 3 And Table 1.

[0109] Table 1 Comparison of clinical efficacy between the two groups

[0110]

[0111] 2. Comparison of bone density changes

[0112] Table 2 Comparison of bone density between the two groups

[0113]

[0114] Compared with the group before treatment, * P<0.05, compared with the control group after treatment, # P<0.05.

[0115] Before treatment, there was no significant difference in bone density between the two groups. After 6 months of treatment, the L 1－4 The improvement of bone density and left femoral neck bone density was higher than that of the control group, with significant difference.

[0116] 3. Adverse reactions

[0117] During the treatment, no adverse reactions such as nausea and vomiting, abnormal liver and kidney function, and anorexia occurred in both groups.

[0118] Bone density measurement is a clinical tool for evaluating bone strength and is recognized as the gold standard for diagnosing osteoporosis. Bone density was tested using bone density measurement, and the results showed that the kidney-tonifying and blood-activating prescription of the present invention can increase bone density, improve bone quality, and improve the clinical symptoms of osteoporosis patients.

[0119] The above embodiments are merely examples for clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description, and the obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis, characterized in that: The kidney-tonifying and blood-activating prescription is prepared from the following raw materials in parts by weight: 5-15 parts of cooked aconite root, 15-25 parts of cooked rehmannia root, 10-20 parts of Morinda officinalis, 5-15 parts of curculigo, 15-25 parts of salvia miltiorrhiza and 4-8 parts of liquorice.

2. The kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis according to claim 1, characterized in that: The kidney-tonifying and blood-activating prescription comprises the following raw materials in parts by weight: 10 parts of cooked aconite root, 20 parts of cooked rehmannia root, 15 parts of Morinda officinalis root, 10 parts of Curculigo orchioides, 20 parts of Salvia miltiorrhiza and 6 parts of liquorice.

3. The kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis according to any one of claims 1 or 2, characterized in that: The preparation method of the kidney-tonifying and blood-activating prescription is as follows: weigh each medicinal material, add 6-8 times the amount of water and boil twice, each time for 0.5-1 hour, combine the two decoctions, concentrate to an extract with a relative density of 0.05-1.15, spray dry, and pass the dried fine powder through a 14-16 mesh sieve to obtain the extract.

4. The kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis according to any one of claims 1 or 2, characterized in that: The preparation method of the kidney-tonifying and blood-activating prescription is as follows: weigh each medicinal material, add 8 times the amount of water and boil twice, each time for 1 hour, combine the two decoctions, concentrate to an extract with a relative density of 1.15, spray dry, and pass the dried fine powder through a 16-mesh sieve to obtain the extract.

5. The kidney-tonifying and blood-activating prescription for preventing and treating osteoporosis according to any one of claims 1 or 2, characterized in that: The preparation of the kidney-tonifying and blood-activating prescription is in the form of decoction, pill, gel, tablet, mixture, capsule, granule, powder or paste.

6. Use of the Bushen Huoxue prescription for preventing and treating osteoporosis according to any one of claims 1 or 2 in the preparation of a drug for preventing or treating osteoporosis.

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