A hydrogel capable of regulating stem cell differentiation and its preparation method and application

The dECM-ODex hydrogel synthesized by Schiff base reaction solves the problem of regulating hydrogel stiffness and viscoelasticity, promotes the differentiation of stem cells in liver tissue engineering, and improves the treatment effect of liver diseases, especially acute liver failure.

CN119185658BActive Publication Date: 2025-09-16SUN YAT SEN UNIV +1
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Patent Information

Application Number
CN202411255854.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-09
Publication Date
2025-09-16
Estimated Expiration
2044-09-09

AI Technical Summary

Technical Problem

Existing synthetic hydrogels have the problem of difficulty in independently adjusting stiffness and viscoelasticity in regulating stem cell differentiation, making it difficult to simulate the mechanical properties of the extracellular microenvironment, resulting in inaccurate liver-directed differentiation of stem cells and high cost.

Method used

The dECM-ODex hydrogel formed by decellularized matrix and oxidized dextran was synthesized by Schiff base reaction, which regulated the stiffness and viscoelasticity of the hydrogel, simulated the mechanical environment of liver tissue, promoted stem cell differentiation, and improved liver disease through biocompatibility and degradability.

Benefits of technology

It has achieved precise regulation of stem cell differentiation in liver tissue engineering, promoted the function of hepatocyte-like cells, improved the symptoms of liver diseases, especially acute liver failure, and has good biocompatibility and degradability.

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Abstract

The present invention relates to a hydrogel capable of regulating stem cell differentiation, a preparation method and an application thereof, and belongs to the field of biological tissue medical engineering technology. The present invention utilizes decellularized matrix (dECM) and oxidized dextran (ODex) to synthesize dECM-ODex hydrogel capable of independently regulating stiffness and viscoelasticity. dECM contains a large amount of collagen, which forms the main gel network skeleton when polymerized, and there is a weak Schiff base reaction between dECM and ODex. By maintaining the concentration of dECM hydrogel while regulating the number of aldehyde groups of ODex (i.e., the molar ratio of NaIO4 to Dex and the ODex concentration), the mechanical properties of dECM-ODex hydrogel, including viscoelasticity and stiffness, can be adjusted to simulate the mechanical environment of liver tissue. The synthesized dECM-ODex hydrogel can regulate the differentiation of stem cells and treat liver diseases. The present invention provides a new tissue engineering material option for the treatment of liver diseases.
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