Traditional Chinese medicine composition for tonifying kidney yang as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for tonifying kidney yang and a preparation method and application thereof.The traditional Chinese medicine composition comprises, by weight, 25-35 parts of eucommia ulmoides, 10-20 parts of radix rehmanniae, 8-10 parts of photinia serrulata, 5-7 parts of notopterygium roots, 5-7 parts of processed radix aconiti lateralis preparata, 8-12 parts of radix achyranthis bidentatae, 8-12 parts of cortex lycii radicis, 8-12 parts of cortex acanthopanacis, 8-12 parts of coix seeds, 8-12 parts of ligusticum wallichii and 5-7 parts of liquorice. The traditional Chinese medicine composition disclosed by the invention is used for preparing a medicine for tonifying kidney yang, especially for treating lumbosacral joint strain and degenerative arthritis caused by deficiency of liver and kidney and external invasion of rheumatism; lumbar muscle strain and sciatica caused by wind toxin attacking and pricking of the kidney; the traditional Chinese medicine composition has a good treatment effect on dysmenorrhea and irregular menstruation caused by liver and kidney deficiency, cold coagulation and blood stasis of women.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicine compositions, and particularly relates to a Chinese medicine composition for tonifying kidney yang, a preparation method and use thereof. Background Art

[0002] Kidney Yang deficiency syndrome refers to a type of deficiency and cold symptoms caused by excessive deficiency of Yang Qi in the body and dysfunction of warming function. This syndrome can occur in many diseases during their development, such as impotence, insomnia, late diabetes, functional constipation in the elderly, primary osteoporosis and perimenopausal syndrome. Under the guidance of the idea of ​​treating different diseases with the same treatment, although the diseases are different, the same treatment method is usually used when they all show symptoms of kidney Yang deficiency, and there are also the same or similar prescriptions. At present, there are a large number of Chinese patent medicines on the market that can treat diseases with kidney Yang deficiency syndrome characteristics, including: Fufang Xuanju Capsule, Jinkui Shenqi Pills, Shenbao Mixture, etc.

[0003] The "disease-symptom-drug" supernetwork analysis and core prescription findings of marketed Chinese patent medicines for the treatment of kidney yang deficiency syndrome, Yu Chengcheng et al., Chinese Herbal Medicine, Volume 54, Issue 24, December 2023, disclosed the frequency statistics of Chinese medicines for the treatment of kidney yang deficiency syndrome, in order: Epimedium, Rehmannia, Lycium, Pilose Antler, Ginseng, Cistanche, Cuscuta, Psoralea, Astragalus, Angelica, Cinnamon, Morinda, Eucommia, Schisandra, Poria, Achyranthes, Aconite, Cynomorium, Table 3 also discloses the selection of Chinese medicines for different symptoms, and Table 8 also discloses the compatibility of Chinese medicines for major diseases.

[0004] Patent application CN104474114A discloses a Chinese medicine composition for treating kidney-yang deficiency type lumbar muscle strain and its preparation method, wherein the oral medicine comprises the following raw materials in parts by weight: 10-20 parts of astragalus, 10-20 parts of tuckahoe, 10-20 parts of prepared radix polygoni multiflori, 10-20 parts of gentiana macrophylla, 10-20 parts of prepared rehmannia, 10-20 parts of dipscutellaria, 10-20 parts of codonopsis pilosula, 10-20 parts of cyathula capitata, 10-20 parts of cassia twig, 10-20 parts of angelica dahurica, 10-20 parts of mistletoe, 10-20 parts of cinnamon bark, 10-20 parts of polygonum cuspidatum, 10-20 parts of eucommia, 10-20 parts of cibotium bark, 10-20 parts of schizonepeta tenuifolia, 10-20 parts of drynaria, 10-20 parts of salvia miltiorrhiza, 10-20 parts of old crane herb, 10-20 parts of red peony root, 20 parts, Corydalis 10-20 parts, Eupolyphaga sinensis 10-20 parts, Psoralea corylifolia 10-20 parts. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a Chinese medicine composition for tonifying kidney yang, a preparation method and use thereof, so as to improve the therapeutic effect thereof.

[0006] The embodiment of the present invention provides a Chinese medicine composition for tonifying kidney yang, comprising the following Chinese medicine components in parts by weight: 25-35 parts of Eucommia ulmoides, 10-20 parts of Rehmannia glutinosa, 8-10 parts of Photinia serrulata, 5-7 parts of Notopterygium wilfordii, 5-7 parts of Radix Aconiti Lateralis Preparata, 8-12 parts of Achyranthes bidentata, 8-12 parts of Lycium bark, 8-12 parts of Acanthopanax acanthopanax, 8-12 parts of Coix seeds, 8-12 parts of Ligusticum chuanxiong, 5-7 parts of Liquorice, 15-25 parts of Pittosporum tobira bark, 12-18 parts of Carthamus tinctorius, 12-18 parts of Sambucus salsa, and 10-15 parts of Corydalis yanhusuo.

[0007] Preferably, the Chinese medicinal components are included in the following parts by weight: 28-32 parts of Eucommia ulmoides, 13-27 parts of Rehmannia glutinosa, 8-10 parts of Photinia serrulata, 5-7 parts of Notopterygium wilfordii, 5-7 parts of Radix Aconiti Lateralis Preparata, 8-12 parts of Achyranthes bidentata, 8-12 parts of Lycium bark, 8-12 parts of Acanthopanax acanthopanax, 8-12 parts of Coix seeds, 8-12 parts of Ligusticum chuanxiong, 5-7 parts of Liquorice, 17-23 parts of Pittosporum tobira bark, 13-17 parts of Carthamus tinctorius, 13-17 parts of Sambucus salsa, and 10-14 parts of Corydalis yanhusuo.

[0008] Preferably, the Chinese medicinal components are included in the following parts by weight: 30 parts of Eucommia ulmoides, 15 parts of Rehmannia glutinosa, 9 parts of Photinia serrulata, 6 parts of Notopterygium wilfordii, 6 parts of Aconitum carmichaelii, 10 parts of Achyranthes bidentata, 10 parts of Lycium bark, 10 parts of Acanthopanax cortex, 10 parts of Coix seed, 10 parts of Ligusticum chuanxiong, 6 parts of Liquorice, 20 parts of Pittosporum tobira bark, 15 parts of Carthamus tinctorius, 15 parts of Sambucus salsa, and 12 parts of Corydalis yanhusuo.

[0009] In one embodiment, the dosage form of the Chinese medicine composition is decoction, tablet, pill, powder, paste, granule, capsule or oral liquid.

[0010] The embodiment of the present invention provides a method for preparing the Chinese medicine composition, comprising mixing the Chinese medicine components, adding 8-12 times the weight of water, decocting to obtain a decoction and medicinal residues, adding 4-8 times the weight of water to the medicinal residues and decocting, combining the decoctions, and obtaining the Chinese medicine composition.

[0011] Preferably, the various Chinese medicinal components are mixed, 10 times the weight of water is added, and decocted to obtain a decoction and medicinal residues, and the medicinal residues are decocted with 6 times the weight of water, and the decoctions are combined to obtain the Chinese medicinal composition.

[0012] The embodiment of the present invention provides a use of the traditional Chinese medicine composition, wherein the traditional Chinese medicine composition is used to prepare a medicine for tonifying kidney yang.

[0013] Preferably, the drug for invigorating kidney yang is a drug for treating the following diseases: liver and kidney deficiency, lumbar and sacral joint strain and degenerative arthritis caused by rheumatism invasion; lumbar muscle strain and sciatica caused by wind poison attacking the kidney; dysmenorrhea and irregular menstruation caused by liver and kidney deficiency, cold coagulation and blood stasis in women.

[0014] The invention has the beneficial effect that the prescription of the invention is as follows: Eucommia ulmoides and Radix Rehmanniae are used as the main ingredients, Eucommia ulmoides and Radix Rehmanniae are used to nourish the liver and kidney, strengthen the waist and knees, Radix Rehmanniae are used to clear away heat and cool blood, nourish yin and produce body fluid; Notopterygium wilfordii, Photinia prunifolia and Semen Coix are used as the assistant ingredients to dispel wind and dampness, Achyranthes bidentata, Cortex Acanthopanacis, Cortex Pittosporum and Herba Simmondsiae are used to dispel rheumatism, strengthen tendons and bones, and relieve arthralgia and pain, Rhizoma Chuanxiong and Safflower are used to promote blood circulation and dissipate blood stasis, promote menstruation and relieve pain, Rhizoma Corydalis are used to promote blood circulation, promote qi circulation and relieve pain, Radix Aconiti Lateralis Preparata warms yang and drives away cold, and Radix Rehmanniae is used as the auxiliary ingredient to cool the heat in the blood. The whole prescription has the effects of warming and nourishing the liver and kidney, strengthening tendons and bones, dispelling wind and relieving pain, and promoting blood circulation and dissipating blood stasis.

[0015] The invention is mainly used for treating: liver and kidney deficiency, rheumatism invasion, waist and leg pain; kidney stabbed by wind poison, unbearable waist pain; dysmenorrhea caused by liver and kidney deficiency, cold coagulation and blood stasis in women. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Figure 2 shows the swimming paths of rats in each group. DETAILED DESCRIPTION

[0017] Example 1 A Chinese medicine composition for tonifying kidney yang comprises the following Chinese medicine components: 30g of Eucommia ulmoides, 15g of Rehmannia glutinosa, 9g of Photinia serrulata, 6g of Notopterygium wilfordii, 6g of Radix Aconiti Lateralis Preparata, 10g of Achyranthes bidentata, 10g of Cortex Lycii, 10g of Cortex Acanthopanacis, 10g of Semen Coicis, 10g of Rhizoma Chuanxiong, 6g of Licorice, 20g of Cortex Pittosporum, 15g of Carthamus tinctorius, 15g of Sambucus sylvestris, and 12g of Rhizoma Corydalis.

[0018] The preparation method comprises the following steps: mixing the various Chinese medicinal components, adding 10 times the weight of water, decocting to obtain a decoction and medicinal residues, decocting the medicinal residues with 6 times the weight of water, combining the decoctions, and obtaining the Chinese medicinal composition.

[0019] Example 2 1. Materials and Methods 1. Experimental Animals SD rats, male, 200-220 g, 60 rats, were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. and raised in the Experimental Animal Center of Hunan University of Chinese Medicine.

[0020] 2. Medication The Chinese medicine composition of Example 1 is the Eucommia ulmoides prescription.

[0021] Positive medicine: Jinkui Shenqi Pills (rehmannia root, Chinese yam, wine-soaked cornus fruit, Poria, peony bark, oriental water plantain, cinnamon twig, aconite (roasted), Achyranthes bidentata (head removed), plantain seed with salt, and honey as auxiliary material). Produced by Beijing Tongrentang Technology Development Co., Ltd., national medicine standard Z11020147, specification: 360 pills * 0.2 g / pill, human dosage is 9 g / d, and each 1 g pill is equivalent to 9 g of the original drug amount.

[0022] 3. Main reagents and instruments Adenine was purchased from Beijing Solebow Technology Co., Ltd.; testosterone, estradiol, gonadotropin-releasing hormone, luteinizing hormone, 17-hydroxycorticosteroids, and cortisol ELISA kit were purchased from Jiangsu Feiya Biotechnology Co., Ltd.

[0023] The open field box was customized; the Morris water maze was purchased from Chengdu Taimeng Software Co., Ltd.; the 1 / 10,000 precision balance was purchased from Mettler Toledo, Switzerland; the low-temperature high-speed centrifuge was purchased from Eppendorf, Germany; the fully automatic microplate reader was purchased from Thermo, USA; and the optical microscope was purchased from Zeiss, Germany.

[0024] 4. Grouping, Modeling and Drug Administration According to body weight, 60 SD rats were randomly divided into normal group, model group, Eucommia ulmoides high-dose group, Eucommia ulmoides medium-dose group, Eucommia ulmoides low-dose group and positive drug group, with 10 rats in each group. Except for the normal group, the rat kidney yang deficiency model was established by intragastric administration of adenine (200 mg / kg) for 42 consecutive days in the other groups, and the normal group was intragastricly administered with an equal volume of distilled water. The test drugs were given from the 15th day of modeling, and the doses of Eucommia ulmoides high-dose, medium-dose and low-dose groups were 16.56 g / kg, 8.28 g / kg and 4.14 g / kg (1, 0.5 and 0.25 times the clinical equivalent dose for humans), respectively. The positive drug group was intragastricly administered with 0.81 g / kg of Jinkuishenqi Pills (i.e. 7.29 g crude drug / kg, 1 times the clinical equivalent dose for humans), and the normal group and model group were administered with an equal volume of distilled water, with an intragastric volume of 10 mL / kg, once a day for 28 consecutive days. After the administration, the in vivo function indexes of the rats were tested. After the test, the rats were anesthetized, blood was collected, and relevant tissues were separated in turn and stored for later use.

[0025] 5. Index detection 1 General status observation The behavior, hair, feces and stools, drinking water and diet, and bedding moisture of each group of rats were observed and weighed once a week.

[0026] 2 Food and water intake testing Each cage of rats was given 100 g of feed and 400 mL of distilled water. After 24 h, the remaining feed weight and distilled water volume were weighed to calculate the food intake and water intake of rats in each group.

[0027] 3. Rectal temperature detection Place the rat in a holder with its tail exposed. After defecation is complete, insert the thermometer into the anus. After the rat stabilizes, read the value and record it.

[0028] 4. Autonomous activity ability test The rat was placed in the center of a black open box with 25 small grids at the bottom. After 30 seconds of free movement, the number of times it crossed the grids and the number of times it stood vertically in the next 4 minutes were recorded. The sum of the two was used to evaluate the rat's autonomous activity ability.

[0029] 5. Swimming distance detection The rats were placed in the water maze and allowed to swim freely for 30 seconds. The swimming distance in the next 60 seconds was recorded using the SMART 3.0 small animal behavior recording and analysis system.

[0030] 6 Organ Index The tissues were separated and weighed, and the organ index was calculated = organ weight (mg) / body weight (g).

[0031] 7 ELISA experiment After blood collection, the rats were allowed to stand for 1 h, centrifuged at 3000 r / min for 15 min, and the supernatant was taken to obtain serum samples. ELISA kits were used to detect the levels of rat serum testosterone, estradiol, gonadotropin-releasing hormone, luteinizing hormone, 17-hydroxycorticosteroids, and cortisol. The operation process was strictly carried out in accordance with the instructions of the kit. The content of the target factor in each group of samples was calculated according to the standard curve.

[0032] 2. Research Results (1) Body mass Compared with the normal group, the body weight of rats in the model group decreased significantly from the first week ( P <0.01); Compared with the model group, the body weight of rats in the high-dose and medium-dose Eucommia prescription groups increased significantly in the fourth week of the experiment (i.e., 2 weeks after administration) ( P <0.01 or P <0.05), after the end of the administration, the body weight of rats in each administration group increased, among which the difference among the high-dose group, medium-dose group and positive drug group of Eucommia ulmoides was statistically significant ( P <0.01 or P <0.05). See Table 1-1.

[0033] Table 1-1 Effect of Eucommia ulmoides prescription on body weight of rats with kidney yang deficiency model ( ±s,n=10)

[0034] Note: Compared with the normal group** P <0.01,* P <0.05; compared with the model group ## P <0.01, # P <0.05(the same below).

[0035] (2) Water intake, food intake and body temperature Compared with the normal group, the 24-hour water intake of rats in the model group increased significantly, and the 24-hour food intake decreased significantly ( P <0.01); compared with the model group, the 24-hour water intake of rats in the high-dose and medium-dose groups of Eucommia ulmoides and the positive drug group was significantly reduced ( P <0.01), the 24 h food intake of the high and medium dose groups of Eucommia ulmoides prescription increased significantly ( P <0.01 or P <0.05); compared with the normal group, the body temperature of rats in the model group was significantly reduced ( P <0.01); compared with the model group, the body temperature of rats in the high-dose and medium-dose groups of Eucommia ulmoides and the positive drug group was significantly increased ( P <0.05). See Table 1-2.

[0036] Table 1-2 Effects of Eucommia ulmoides prescription on water intake, food intake and body temperature of rats with kidney yang deficiency model ( ±s,n=10)

[0037] (3) Number of spontaneous activities and swimming distance Compared with the normal group, the number of spontaneous activities and swimming distance of rats in the model group were significantly reduced ( P <0.01 or P <0.05); compared with the model group, the number of spontaneous activities and swimming distance in the high and medium dose groups of Eucommia ulmoides were significantly increased ( P <0.01 or P <0.05), among which the average swimming distance of the high-dose group was greater than that of the normal group. Figure 1 , Tables 1-3.

[0038] Table 1-3 Effect of Eucommia ulmoides prescription on the number of spontaneous activities and swimming distance of rats with kidney yang deficiency model ( ±s,n=10)

[0039] (4) Organ index In terms of organ indexes, compared with the normal group, the kidney index and testicular index of the rats in the model group increased significantly ( P <0.01), the seminal vesicle index decreased ( P <0.01 or P <0.05); compared with the model group, only the epididymal index of the high-dose group increased ( P <0.05), the seminal vesicle index increased in the medium dose group of Eucommia ulmoides prescription ( P <0.05), the renal index of each group was significantly reduced ( P <0.01 orP <0.05, and the high and medium dose groups of Eucommia ulmoides were the most effective ( P <0.001). See Table 1-4.

[0040] Table 1-4 Effects of Eucommia ulmoides prescription on organ indexes of rats with kidney yang deficiency model ( ±s,n=10)

[0041] (5) Serum-related indicators Compared with the normal group, the levels of serum gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), testosterone (T), and cortisol (Cortisol) in the model group were significantly decreased ( P <0.01 or P <0.05), 17-hydroxycorticosteroid (17-OHCS) content increased ( P <0.01); compared with the model group, the GnRH content in the high, medium and low dose groups of Eucommia ulmoides was increased ( P <0.05), the LH content in the high and low dose groups of Eucommia ulmoides prescription increased ( P <0.05), the T content in the high, medium and low dose groups of Eucommia ulmoides prescription and the positive drug group increased ( P <0.01 or P <0.05), the 17-OHCS content in the high, medium and low dose groups of Eucommia ulmoides prescription decreased ( P <0.01 or P <0.05), the Cortisol content in the high, medium and low dose groups of Eucommia ulmoides prescription and the positive drug group increased ( P <0.01 or P <0.05). See Table 1-5.

[0042] Table 1-5 Effects of Eucommia ulmoides prescription on serum-related indices in rats with kidney yang deficiency model ( ±s,n=6)

[0043] GnRH: gonadotropin-releasing hormone; LH: luteinizing hormone; E2: estradiol; T: testosterone; 17-OHCS: 17-hydroxycorticosteroid; Cortisol: cortisol.

[0044] Example 3 1. Materials and Methods 1. Experimental Animals ICR mice, male, 18-20 g, 90 mice, were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd. and raised in the Experimental Animal Center of Hunan University of Chinese Medicine.

[0045] 2. Medication The Chinese medicine composition of Example 1 is the Eucommia ulmoides prescription.

[0046] Positive medicine: Jinkui Shenqi Pills (rehmannia root, Chinese yam, wine-soaked cornus fruit, Poria, peony bark, oriental water chestnut, cinnamon twig, aconite (roasted), Achyranthes bidentata (head removed), plantain seed with salt, and honey as auxiliary material). Produced by Beijing Tongrentang Technology Development Co., Ltd., national medicine standard Z11020147, specification: 360 pills * 0.2 g / pill, human dose is 9 g / d.

[0047] 3. Grouping and Dosing According to body weight, 90 male ICR mice were randomly divided into normal group, high-dose Eucommia Recipe group, medium-dose Eucommia Recipe group, low-dose Eucommia Recipe group, and positive drug group, with 10 mice in each group. The high, medium, and low doses of Eucommia Recipe were 23.92 g / kg, 11.96 g / kg, and 5.98 g / kg (1, 0.5, and 0.25 times the clinical equivalent dose for humans), respectively. The positive drug group was given Jinkui Shenqi Pills 1.17 g / kg (i.e., 10.53 g crude drug / kg, 1 times the clinical equivalent dose for humans) by gavage, and the normal group was given an equal volume of distilled water, with a gavage volume of 20 mL / kg, once a day for 14 consecutive days.

[0048] 4. Statistical Analysis 1 Body mass The mice were weighed before administration and on the 7th and 14th days after administration to observe the changes in body weight of mice in each group.

[0049] 2 Food and water intake Each cage of mice was given 100 g of feed and 100 mL of distilled water. After 24 h, the remaining feed weight and distilled water volume were weighed to calculate the 24 h food intake and water intake of mice in each group.

[0050] 3. Number of independent activities This experiment mainly examines the effect of drugs on tonifying kidney yang. Mice were placed in an open field box of 40 cm×40 cm×40 cm, with the bottom divided into 16 small grids. Mice were placed from the center and allowed to move freely for 1 min to adapt to the environment. The number of grids crossed and the number of vertical standing times of the mice in the next 3 min were recorded, and the sum of the two was used to represent their autonomous activity score.

[0051] 4 Exhaustive swimming test This experiment mainly investigates the anti-fatigue effect of drugs. A lead sheet weighing 10% of the body weight of the mouse was tied to the end of its tail, and the mouse was placed in a swimming box (600*440*330 mm) at 25°C and about 25 cm deep for weighted swimming. The time when the mouse first sank and the time when it sank for 3 seconds without floating (exhaustion time) were recorded.

[0052] 5 Sexual function experiments This experiment mainly investigates the efficacy of drugs in tonifying kidney yang and improving sexual function. The experiment was conducted at night, the room lights were dimmed, male mice were placed in cages for 5 minutes in advance, and two female mice were put in. The time of the first mounting (mounting latency) and the number of mountings, the time of the first mating (mating latency) and the number of matings within 20 minutes were recorded. If the mice did not mount or mate within 20 minutes, the corresponding latency was recorded as 1200 seconds.

[0053] 5. Statistical Analysis SPSS 19.0 software was used for data analysis. The experimental data in this study were expressed as mean ± standard deviation ( ± s) and one-way ANOVA was used. LSD test was used for multiple comparisons when variance was equal, and Tambane's T test was used when variance was unequal. 2 test. P <0.05 indicated that the difference was statistically significant.

[0054] 2. Research Results (1) Body mass Compared with the normal group, the body weight of mice in the high-dose Eucommia recipe group and the positive drug group increased significantly after 2 weeks of administration ( P <0.01 or P <0.05), there was no statistical difference in the other groups. See Table 2-1.

[0055] Table 2-1 Effect of Eucommia ulmoides prescription on body weight of mice ( ±s, n=10)

[0056] Note: Compared with the normal group** P <0.01,* P <0.05. (The same below) (2) Water and food intake Compared with the normal group, the water intake of mice in the high-dose Eucommia recipe group increased significantly ( P <0.05), the food intake of the high-dose and medium-dose groups of Eucommia ulmoides and the positive drug group increased significantly ( P <0.01 or P <0.05). The results showed that high dose of Eucommia ulmoides prescription can promote mice to drink water and eat, and has a good effect of improving appetite. See Table 2-2.

[0057] Table 2-2 Effects of Eucommia ulmoides prescription on drinking water and food intake of mice ( ±s, n=10)

[0058] (3) Number of spontaneous activities Compared with the normal group, after drug intervention, only the high-dose group of Eucommia ulmoides could increase their activity, but there was no statistical difference. The results showed that Eucommia ulmoides had no significant effect in promoting the activity of normal mice. See Table 2-3.

[0059] (4) Swimming time to exhaustion Compared with the normal group, each drug group had no significant improvement on the first sinking time of mice in exhaustive swimming; the high-dose group of Eucommia ulmoides prescription could significantly prolong the exhaustive time of mice ( P <0.05). The results showed that high dose of Eucommia ulmoides prescription could prolong the exhaustive swimming time of mice and had good anti-fatigue effect. See Table 2-3.

[0060] Table 2-3 Effect of Eucommia ulmoides prescription on exhaustive swimming time of mice ( ±s, n=10)

[0061] (5) Sexual function Compared with the normal group, the high-dose group of Eucommia ulmoides could significantly increase the number of mice mounting and reduce the mounting latency ( P <0.05), and can increase the number of matings, but due to the large individual differences, the results were not statistically significant; the positive drug group could only significantly increase the mouse mounting latency ( P <0.05). This indicates that high dose of Eucommia ulmoides prescription has a certain effect in improving sexual function. See Table 2-4.

[0062] Table 2-4 Effects of Eucommia ulmoides prescription on sexual function indexes of mice ( ±s, n=10)

[0063] A person skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of protection of the present application is limited to these examples. In line with the concept of the present application, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of different aspects of one or more embodiments of the present application as described above, which are not provided in detail for the sake of simplicity.

[0064] One or more embodiments of the present application are intended to cover all such substitutions, modifications and variations that fall within the broad scope of the present application. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of one or more embodiments of the present application should be included in the protection scope of the present application.

Claims

1. A Chinese medicine composition for tonifying kidney yang, characterized in that: The invention comprises the following traditional Chinese medicine components in parts by weight: 25-35 parts of Eucommia ulmoides, 10-20 parts of Rehmannia glutinosa, 8-10 parts of Photinia serrulata, 5-7 parts of Notopterygium wilfordii, 5-7 parts of Radix Aconiti Lateralis Preparata, 8-12 parts of Achyranthes bidentata, 8-12 parts of Lycium bark, 8-12 parts of Acanthopanax acanthopanax, 8-12 parts of Coix seeds, 8-12 parts of Ligusticum chuanxiong, 5-7 parts of Liquorice, 15-25 parts of Pittosporum tobira bark, 12-18 parts of Carthamus tinctorius, 12-18 parts of Sambucus salsa, and 10-15 parts of Corydalis yanhusuo.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention comprises the following traditional Chinese medicine components in parts by weight: 28-32 parts of Eucommia ulmoides, 13-27 parts of Rehmannia glutinosa, 8-10 parts of Photinia serrulatae, 5-7 parts of Notopterygium wilfordii, 5-7 parts of Radix Aconiti Lateralis Preparata, 8-12 parts of Achyranthes bidentatae, 8-12 parts of Cortex Lycii, 8-12 parts of Cortex Acanthopanacis, 8-12 parts of Semen Coicis, 8-12 parts of Rhizoma Chuanxiong, 5-7 parts of Liquorice, 17-23 parts of Cortex Pittosporum, 13-17 parts of Carthamus tinctorius, 13-17 parts of Sambucus salsa, and 10-14 parts of Rhizoma Corydalis.

3. The Chinese medicine composition as claimed in claim 2, characterized in that: The invention comprises the following traditional Chinese medicine components in parts by weight: 30 parts of eucommia bark, 15 parts of radix rehmanniae, 9 parts of photinia serrulatae, 6 parts of notopterygium wilfordii, 6 parts of roasted aconite root, 10 parts of achyranthes bidentatae, 10 parts of radix rehmanniae, 10 parts of cortex acanthopanacis, 10 parts of coix seed, 10 parts of ligusticum chuanxiong, 6 parts of liquorice, 20 parts of cortex pittosporume, 15 parts of safflower, 15 parts of comfrey, and 12 parts of corydalis.

4. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The dosage form of the Chinese medicine composition is decoction, tablet, pill, powder, paste, granule, capsule or oral liquid.

5. A method for preparing the Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The Chinese medicinal components are mixed, 8-12 times by weight of water are added, and the mixture is boiled to obtain a decoction and medicinal residues. The medicinal residues are boiled with 4-8 times by weight of water, and the decoctions are combined to obtain the Chinese medicinal composition.

6. The preparation method according to claim 5, characterized in that: The Chinese medicinal components are mixed, 10 times the weight of water is added, and the mixture is boiled to obtain a decoction and medicinal residues. The medicinal residues are boiled with 6 times the weight of water, and the decoctions are combined to obtain the Chinese medicinal composition.

7. A use of the Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The Chinese medicine composition is used for preparing medicine for invigorating kidney yang.

8. The use according to claim 7, characterized in that: Drugs that replenish kidney yang are used to treat the following diseases: liver and kidney deficiency, lumbar and sacral joint strain and degenerative arthritis caused by rheumatic invasion; lumbar muscle strain and sciatica caused by wind poison attacking the kidney; dysmenorrhea and irregular menstruation caused by liver and kidney deficiency, cold stagnation and blood stasis in women.

Images