Multifunctional biological preparation and preparation method thereof

Abstract

The present invention relates to the technical field of preservation of human body, animal and plant body or parts thereof, and in particular to a multifunctional biological preparation and a preparation method thereof. The biological preparation comprises the following components in parts by weight: 5-10 parts of microbial agent, 5-15 parts of brown algae oligosaccharide, 1-5 parts of amphiphilic surfactant, 2-6 parts of potassium humate and 30-50 parts of carrier. Compared with the prior art, the combined use of Bacillus subtilis and potassium humate in the multifunctional biological preparation of the present invention can not only promote the formation of soil aggregate structure and increase the organic matter content to improve the soil structure by virtue of the organic matter rich in potassium humate and the decomposition of organic matter by Bacillus subtilis, but also improve the soil fertility and enhance the absorption efficiency of nutrients by plants by virtue of the chelating characteristics of potassium humate and the ability of Bacillus subtilis to decompose minerals, as well as their stimulation of plant root growth and promotion of nutrient absorption.

Description

Technical Field

[0001] The present invention relates to the technical field of preservation of human body, animal and plant body or parts thereof, and in particular to a multifunctional biological preparation and a preparation method thereof. Background Art

[0002] Sustainable agriculture aims to increase food production and stability while optimizing soil quality. Fertilizers play a key role in promoting crop growth and improving soil fertility, but excessive use may damage soil organic matter, reduce soil quality, and cause environmental pollution. Therefore, biofertilizers, organic substances that use microorganisms to improve soil fertility, have attracted widespread attention due to their potential to protect soil health and improve crop quality. Microorganisms in the soil, especially those in the rhizosphere, are closely related to plant growth and soil fertility. The diversity and richness of soil microorganisms are important factors affecting plant growth and ecological stability.

[0003] Biological agents are fertilizers containing active microorganisms. They work by promoting plant absorption of nutrients, enhancing crop growth, and improving soil structure. Biological agents can activate the soil, adjust the acid-base balance, improve soil permeability, and release fixed nutrients, thereby significantly improving nutrient utilization efficiency and reducing dependence on chemical fertilizers. At present, biological agents have been widely used to improve the soil microbial environment and effectively enhance the disease resistance of crops. As a new type of fertilizer, it has multiple functions and obvious advantages, and plays an important role in increasing crop yield and quality and promoting sustainable agricultural development.

[0004] CN112155010A relates to a biological control agent, which is composed of a nano-biological buffer and biological control microorganisms. The nano-biological buffer is composed of A solution and B solution. The A solution is a colloidal solution of nanoparticles, and the B solution is composed of a surfactant, water, an adhesive and a carrier. The nanoparticles in the colloidal solution of nanoparticles are nanoparticles of a copolymer of methyl methacrylate and methacrylic acid. The adhesive is one or more of polyvinyl alcohol PVA, starch, carboxymethyl cellulose and dextrin, the surfactant is one or more of sodium dodecyl sulfate SDS, sodium dioctyl succinate sulfonate, sodium dodecylbenzene sulfonate, lecithin and Tween80, and the carrier is one or more of light calcium carbonate CaCO3, scallop shell powder and eggshell powder. The biological control agent of the invention is conducive to the survival of biological control microorganisms and promotes the release of metabolites and the exertion of disease control capabilities.

[0005] CN111771911A proposes a microbial preparation for preventing and treating tobacco mosaic virus, comprising a microbial mixture, a plant immune inducer and a leaf extract, wherein the leaf extract is a leaf extract of tobacco plants that produce an immune response after being sprayed with the plant immune inducer, wherein the mass percentages of the microbial mixture, the plant immune inducer and the leaf extract are 5% to 10% of the microbial mixture, 3% to 5% of the plant immune inducer and 10% to 15% of the leaf extract, and the remainder is sterile water, wherein the plant immune inducer is a tobacco mosaic virus capsid protein solution; the plant immune inducer in the invention acts as an immune stimulating factor, which can trigger a stress immune response in the plant immune system and induce it to produce immune resistance against tobacco mosaic virus, and the leaf extract contains a large amount of biochemical substances that can limit virus proliferation, and plays a certain role in preventing and treating viruses when the plant's own immune response has not yet fully exerted its effectiveness.

[0006] Currently, the effects of biological agents are relatively single, so developing a multifunctional biological agent will have greater application value. Summary of the invention

[0007] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a multifunctional biological preparation and a preparation method thereof. The combined use of Bacillus subtilis and potassium humate in the multifunctional biological preparation of the present invention can not only promote the formation of soil aggregate structure and increase the organic matter content to improve the soil structure by virtue of the organic matter rich in potassium humate and the decomposition of organic matter by Bacillus subtilis, but also improve soil fertility and enhance the efficiency of plant absorption of nutrients by virtue of the chelating properties of potassium humate and the ability of Bacillus subtilis to decompose minerals, as well as their stimulation of plant root growth and promotion of nutrient absorption.

[0008] Surfactants can significantly reduce the surface tension of water, allowing water or other agrochemicals to be evenly distributed on plant leaves or in the soil. This helps ensure that the water or formulation better covers the plant leaves and soil surface, improving efficiency. Surfactants indirectly optimize the environment for roots to absorb important nutrients such as nitrogen, phosphorus and potassium by ensuring the uniform distribution of water in the soil matrix. This helps to increase nutrient absorption rates, promote healthier plant growth and higher crop yields. They achieve no splashing or rebound by reducing the dynamic surface tension of the solution, and improve the retention of droplets on hydrophobic leaves. However, the adsorption of currently commonly used surfactants on soil can significantly change the physical and chemical properties of the soil, affect the surface potential, effective Hammer constant and ionic strength of soil colloids, change the chemical properties of the soil, thereby affecting the behavior of compounds in the soil, and cannot be degraded, posing a risk of polluting the environment.

[0009] Therefore, the present invention provides a degradable amphiphilic surfactant, which uses biological component cellulose to react with 6-caprolactone, and ring-opening polymerization occurs under the action of a catalyst to form hydrophobic polycaprolactone chains on cellulose, and then continues to react with amino acids to graft hydrophilic groups, and finally obtains a surfactant. Since both cellulose and polycaprolactone can be biodegraded, the prepared surfactant is safer and more environmentally friendly. Since the amphiphilic surfactant can form a variety of aggregates that are easier to adjust in the solution, the adsorption and aggregation tendency is stronger, the critical micelle concentration is lower, and the surface activity is higher, it can promote the absorption, penetration and migration conduction of the preparation in the target root system, and improve the absorption and conduction efficiency of the effective ingredients.

[0010] The present invention provides a multifunctional biological agent, which can significantly regulate the healthy growth of crops, promote the large-scale expansion of beneficial microorganisms in the soil, promote the robust growth of crops, and greatly improve the tillage of soil. Strengthen the metabolic function of crops and stimulate the growth potential of crops. The crop stems are strong, the leaves are dark green, and the growth is strong. Effectively improve the undesirable problems such as weak seedlings and slow growth, and the seedlings are uniform, strong, and vigorous. Induce and stimulate the immune induction system of crops themselves, improve the ability of crops to resist diseases, and reduce the occurrence of diseases such as root rot, sudden wilting, damping off, and stem base rot. Improve the resistance to premature aging and stress resistance of crops, and make crops resistant to adverse effects such as drought, floods, and low temperatures. Reasonable application of this product can significantly improve the yield and quality of crops. The added brown algae oligosaccharide is conducive to improving and optimizing the soil environment, promoting the doubling of the number of beneficial microbial flora in the soil and the formation of soil aggregate structure, strengthening the soil's water and fertilizer retention capacity, and improving the air permeability, improving the suitability of soil for continuous cropping. Amphiphilic surfactants can promote the absorption, penetration, migration and conduction of agents in the target root system, and improve the absorption and conduction efficiency of effective ingredients. It is adaptable to various water and soil qualities, has good biodegradability, and is beneficial to soil environmental protection.

[0011] To achieve the above-mentioned object, the present invention provides a multifunctional biological preparation, comprising the following components in parts by weight: 5 to 10 parts of microbial agent, 5 to 15 parts of brown algae oligosaccharide, 1 to 5 parts of amphiphilic surfactant, 2 to 6 parts of potassium humate and 30 to 50 parts of carrier.

[0012] The preparation method of the amphiphilic surfactant comprises the following steps:

[0013] S1, dispersing cellulose in a medium, adding 6-caprolactone, benzyl alcohol and tetraphenyltin, and polymerizing by heating to obtain modified cellulose;

[0014] S2. Dispersing the modified cellulose in a medium, adding amino acids to hydrophilize it, neutralizing the pH, and post-treating it to obtain an amphiphilic surfactant.

[0015] Furthermore, the preparation method of the amphiphilic surfactant comprises the following steps:

[0016] S1. Add cellulose to toluene and disperse evenly, then add 6-caprolactone, benzyl alcohol and tetraphenyltin, heat to 110-130°C and stir for 20-30h, then filter to obtain modified cellulose;

[0017] S2. Disperse the modified cellulose in N,N-dimethylformamide, add the N,N-dimethylformamide solution of amino acid, heat to 50-70°C, stir for 4-10 hours and filter, disperse the residue in water, add sodium hydroxide aqueous solution to adjust the pH, add ethanol to dilute, produce precipitation and centrifuge to obtain the amphiphilic surfactant.

[0018] Furthermore, the volume ratio of cellulose to toluene is 1:20-30.

[0019] Furthermore, the mass ratio of the cellulose to 6-caprolactone is 1:5-20.

[0020] Furthermore, the benzyl alcohol and tetraphenyltin are both 0.1-0.2 wt % of 6-caprolactone.

[0021] Furthermore, the mass ratio of the modified cellulose to the amino acid is 1:5-10.

[0022] Furthermore, the volume ratios of the modified cellulose to N,N-dimethylformamide, and the volume ratios of the amino acid to N,N-dimethylformamide are 1:10-20 and 1:2-5, respectively.

[0023] Preferably, the amino acid is one of taurine, alanine or glycine.

[0024] Furthermore, the microbial agent is one or more of Bacillus subtilis, Bacillus amyloliquefaciens, Rhodopseudomonas palustris, Bacillus plantarum, and Aspergillus niger.

[0025] Furthermore, the number of viable bacteria in the preparation is greater than 1×10 10 cfu / g.

[0026] Furthermore, the carrier is one or more of calcium carbonate, eggshell powder, feather powder or shell powder.

[0027] A method for preparing a multifunctional biological agent comprises the following steps:

[0028] The multifunctional biological preparation can be obtained by uniformly mixing the microbial agent, brown algae oligosaccharide, amphiphilic surfactant, potassium humate and carrier according to a proportion.

[0029] Beneficial effects of the present invention:

[0030] 1. Compared with the prior art, the multifunctional biological preparation of the present invention can significantly regulate the healthy growth of crops, promote the large-scale expansion of beneficial microorganisms in the soil, induce and stimulate the immune resistance system of crops themselves, improve the ability of crops to resist diseases, and reduce the occurrence of diseases such as root rot, sudden wilt, damping-off, and stem base rot. The added brown oligosaccharides are beneficial to improving and optimizing the soil environment, promoting the doubling of the number of beneficial microbial flora in the soil and the formation of soil aggregate structure, enhancing the soil's ability to retain water and fertilizer, and improving air permeability, thereby improving the suitability of soil for continuous cropping.

[0031] 2. The present invention provides a degradable amphiphilic surfactant, which can promote the absorption, penetration and migration of the agent in the target root system, and improve the absorption and conduction efficiency of the active ingredients. It is suitable for various water and soil qualities, has good biodegradability, and is beneficial to soil environmental protection. DETAILED DESCRIPTION

[0032] Cellulose, powder, ≤15 μm, product number: SYLG068, Hubei Shuaiyan Ligao Biopharmaceutical Co., Ltd.

[0033] Bacillus subtilis CGMCC 1.821, Bacillus amyloliquefaciens CGMCC 1.857, and Rhodopseudomonas palustris CGMCC1.8929 are commercially available products sourced from the China General Microbiological Culture Collection and Management Center.

[0034] Lactobacillus plantarum CICC 21794 and Aspergillus niger CICC 40265 are commercially available products sourced from the China Industrial Microbiology Culture Collection and Management Center.

[0035] Potassium humate comes from Shanxi Linhai Humic Acid Technology Co., Ltd., product name: Potassium Flavour Tablets.

[0036] Example 1

[0037] A method for preparing a multifunctional biological agent comprises the following steps, measured in parts by weight:

[0038] The multifunctional biological preparation is obtained by uniformly mixing 2 parts of Bacillus subtilis, 2 parts of Bacillus amyloliquefaciens, 1 part of Rhodopseudomonas palustris, 2 parts of Bacillus plantarum, 2 parts of Aspergillus niger, 10 parts of brown algae oligosaccharide, 3 parts of amphiphilic surfactant, 4 parts of potassium humate and 40 parts of eggshell powder.

[0039] The preparation method of the amphiphilic surfactant comprises the following steps, measured in parts by weight:

[0040] S1, add 5 parts of cellulose to 25 times the volume of toluene, disperse evenly, then add 15 parts of 6-caprolactone, 0.03 parts of benzyl alcohol and 0.03 parts of tetraphenyltin, heat to 120°C, stir for 24 hours and filter to obtain modified cellulose;

[0041] S2. Disperse the modified cellulose in 15 times the volume of N,N-dimethylformamide, and then add alanine in N,N-dimethylformamide solution, wherein alanine is 8 parts and the volume of N,N-dimethylformamide is 4 times that of alanine. Heat to 60°C, stir for 6 hours and filter. Disperse the residue in water, add 0.5 mol / L sodium hydroxide aqueous solution to adjust the pH to 8, and then add ethanol to dilute. After precipitation, centrifuge and obtain the amphiphilic surfactant.

[0042] Example 2

[0043] It is basically the same as Example 1, the only difference being that alanine is replaced by glycine.

[0044] Example 3

[0045] It is basically the same as Example 1, the only difference being that alanine is replaced by taurine.

[0046] Example 4

[0047] The method is basically the same as Example 1, except that the amount of the amphiphilic surfactant is 2 parts.

[0048] Example 5

[0049] The method is basically the same as Example 1, except that the amount of the amphiphilic surfactant is 4 parts.

[0050] Comparative Example 1

[0051] A method for preparing a multifunctional biological agent comprises the following steps, measured in parts by weight:

[0052] The multifunctional biological preparation is obtained by uniformly mixing 2 parts of Bacillus subtilis, 2 parts of Bacillus amyloliquefaciens, 1 part of Rhodopseudomonas palustris, 2 parts of Bacillus plantarum, 2 parts of Aspergillus niger, 10 parts of brown algae oligosaccharide, 4 parts of potassium humate and 40 parts of eggshell powder.

[0053] Comparative Example 2

[0054] A method for preparing a multifunctional biological agent comprises the following steps, measured in parts by weight:

[0055] The multifunctional biological preparation is obtained by uniformly mixing 2 parts of Bacillus subtilis, 2 parts of Bacillus amyloliquefaciens, 1 part of Rhodopseudomonas palustris, 2 parts of Bacillus plantarum, 2 parts of Aspergillus niger, 10 parts of brown algae oligosaccharide, 3 parts of Tween 80, 4 parts of potassium humate and 40 parts of eggshell powder.

[0056] Test Example 1

[0057] The disease resistance test was conducted in an area where cotton cropping is serious, soil compaction is serious due to unreasonable use of chemical fertilizers, and soil-borne diseases are prevalent. The test crop is cotton. The multifunctional biological preparation prepared by the present invention and organic fertilizer are mixed and applied at a mass ratio of 1:500, combined with land preparation and turned into the ground. The multifunctional biological preparation is used in an amount of 3 kg per mu. The adjacent plots are used as controls without the application of the present invention. The area of ​​each plot is 100 m 2 , repeated three times. All other managements were the same as usual. The effect of seedling disease control was investigated 10 days, 20 days, 30 days, and 45 days after planting. The seedling diseases such as damping-off, damping-off, root rot, and red rot caused by soil-borne diseases, late spring cold, air temperature, ground temperature, and high humidity were investigated and counted.

[0058] Table 1 Disease resistance test of multifunctional biological agents

[0059]

[0060] Bacillus subtilis can successfully colonize in the rhizosphere, body surface or body of plants, compete with pathogens for nutrients around the plants, secrete antimicrobial substances to inhibit the growth of pathogens, and induce the plant defense system to resist the invasion of pathogens, thereby achieving the purpose of biological control.

[0061] Bacillus amyloliquefaciens can decompose insoluble trace elements in the environment and improve the utilization rate of nutrients in the rhizosphere of plants. The growth-promoting substances it produces can be used by crops to promote crop growth and improve stress resistance; it can also reduce the damage of pathogens to crops by inducing crop defense responses.

[0062] Rhodopseudomonas palustris, Bacillus plantarum, Aspergillus niger, Bacillus subtilis and Bacillus amyloliquefaciens can have significant preventive and therapeutic effects on plant diseases, especially seedling diseases, through various mechanisms such as competing for nutrients, secreting antibacterial substances, and inducing plant defense responses.

[0063] From the data in Table 1, it can be seen that the multifunctional biological agent of the present invention can enhance the metabolic function of crops, stimulate the growth potential of crops, induce and stimulate the immune resistance system of crops themselves, improve the ability of crops to resist diseases, and reduce the occurrence of diseases such as root rot, damping-off, damping-off, and stem base rot.

[0064] Compared with the control example, the anti-disease effect of the embodiment is significantly stronger, which may be due to the addition of amphiphilic surfactants in the embodiment, which can promote the absorption, penetration and migration conduction of the agent in the target root system and improve the absorption and conduction efficiency of the effective ingredients. Compared with ordinary surfactants such as Tween 80, the surfactants in the embodiment have higher surface activity, and the amphiphilic structure leads to better activity at the water / air interface, which can more effectively reduce the surface tension of water. The CMC of amphiphilic surfactants is usually lower than that of traditional surfactants, which means that they can form micelles at lower concentrations, so as to play a more effective role. The aggregation behavior of amphiphilic surfactants in aqueous solutions is more complex and changeable, which makes them have unique advantages in a variety of applications. Amphiphilic surfactants can increase the solubility of certain insoluble substances in water, which is very important for improving the stability and effect of the product. Therefore, the anti-disease ability of the preparations in the embodiment is better. Compared with Example 1, Example 3 may have better solubilization and surface tension reduction effects because taurine has a more water-soluble sulfonic acid group, so the infection rate of plants is lower. Compared with Examples 4 and 5, there are differences in the anti-disease effect of Example 1, and the dosage in Example 1 is relatively the best.

[0065] Test Example 2

[0066] A soil improvement test was conducted using a multifunctional biological agent, and the test field soil was used as the experimental object. The soil was sprayed with the present invention diluted 60 times, and the control group was sprayed with distilled water. The soil organic matter content, total nitrogen content, total phosphorus content, total potassium content, water content, and bulk density of the control group and the experimental group were measured respectively. The specific results are shown in Table 2.

[0067] Table 2 Soil improvement test of multifunctional biological agents

[0068]

[0069] As can be seen from Table 2, the multifunctional biological preparation prepared by the present invention has the best improvement effect on soil, because Bacillus subtilis and Bacillus amyloliquefaciens can secrete multiple enzymes, such as protease, lipase, cellulase, etc., which help to decompose the anti-nutritional factors in plant feed, supplement the deficiency of animal endogenous enzymes, and improve the utilization rate of feed nutrients. In the soil, these microorganisms may promote the decomposition of organic matter and the circulation of nutrient elements. Rhodopseudomonas palustris has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, and produces organic acids, amino acids, polysaccharides, hormones, etc., which are conducive to plant absorption and utilization during growth and reproduction.

[0070] Plant lactobacillus can produce organic acids, special enzymes, acid bacteria and other substances through fermentation, which have special physiological functions, promote animal growth, regulate normal gastrointestinal flora, and maintain microecological balance. In the soil, these functions may help improve the soil microbial environment and promote the healthy growth of plants.

[0071] Aspergillus niger has a variety of enzyme systems that can utilize starch substances and has a certain decomposition ability for proteins, tannins, cellulose, pectin, etc., which helps to decompose organic matter in the soil and release nutrients.

[0072] Potassium humate is an organic macromolecular colloid with strong water absorption and water storage functions. It can improve soil moisture conditions, increase soil organic-inorganic compositeness, increase soil large-particle water-stable aggregates, and effectively improve the physical structure of the soil.

[0073] Eggshell powder contains ingredients such as calcium carbonate, protein, magnesium carbonate and calcium phosphate, which can supplement calcium and other minerals in the soil, promote the formation of soil aggregate structure, improve soil fertility and the mineral absorption capacity of crops.

[0074] Brown algae oligosaccharides are beneficial to improving and optimizing the soil environment, promoting the doubling of the number of beneficial microbial flora in the soil and the formation of soil aggregate structure, strengthening the soil's ability to retain water and fertilizer, improving air permeability, and improving the suitability of soil for continuous cropping. Brown algae oligosaccharides may help improve the soil's water retention capacity and the efficiency of plant absorption of nutrients.

[0075] Amphiphilic surfactants can reduce the surface tension of water and increase the wettability of water, which may help improve the uniformity of water and nutrient distribution in the soil and promote the absorption of water and nutrients by plant roots.

[0076] The reason why the soil in the experimental group has better performance is mainly due to the combined effect of microorganisms and organic components in the multifunctional biological agent, which improves the biological activity, structure, fertility and water retention capacity of the soil, thereby promoting plant growth and soil health. Therefore, the soil in the examples has better performance, and compared with Example 1, Example 3 may have better solubility and surface tension reduction effects due to the taurine having a more water-soluble sulfonic acid group, resulting in better soil improvement effect.

[0077] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multifunctional biological agent, characterized in that: The invention comprises the following components in parts by weight: 5-10 parts of microbial agent, 5-15 parts of brown algae oligosaccharide, 1-5 parts of amphiphilic surfactant, 2-6 parts of potassium humate and 30-50 parts of carrier; The preparation method of the amphiphilic surfactant comprises the following steps: S1, dispersing cellulose in a medium, adding 6-caprolactone, benzyl alcohol and tetraphenyltin, and polymerizing by heating to obtain modified cellulose; S2. Dispersing the modified cellulose in a medium, adding amino acids to hydrophilize it, neutralizing the pH, and post-treating it to obtain an amphiphilic surfactant.

2. The multifunctional biological agent according to claim 1, characterized in that: The preparation method of the amphiphilic surfactant comprises the following steps: S1. Add cellulose to toluene and disperse evenly, then add 6-caprolactone, benzyl alcohol and tetraphenyltin, heat to 110-130°C and stir for 20-30h, then filter to obtain modified cellulose; S2. Disperse the modified cellulose in N,N-dimethylformamide, add the N,N-dimethylformamide solution of amino acid, heat to 50-70°C, stir for 4-10 hours and filter, disperse the residue in water, add sodium hydroxide aqueous solution to adjust the pH, add ethanol to dilute, produce precipitation and centrifuge to obtain the amphiphilic surfactant.

3. The multifunctional biological agent according to claim 2, characterized in that: The volume ratio of the cellulose to toluene is 1:20-30.

4. The multifunctional biological agent according to claim 2, characterized in that: The mass ratio of the cellulose to 6-caprolactone is 1:5-20.

5. The multifunctional biological agent according to claim 2, characterized in that: The benzyl alcohol and tetraphenyltin are both 0.1-0.2 wt % of 6-caprolactone.

6. The multifunctional biological agent according to claim 2, characterized in that: The mass ratio of the modified cellulose to the amino acid is 1:5-10.

7. The multifunctional biological agent according to claim 2, characterized in that: The volume ratios of the modified cellulose to N,N-dimethylformamide and the volume ratios of the amino acid to N,N-dimethylformamide are 1:10-20 and 1:2-5 respectively.

8. The multifunctional biological agent according to claim 1, characterized in that: The microbial agent is one or more of Bacillus subtilis, Bacillus amyloliquefaciens, Rhodopseudomonas palustris, Bacillus plantarum, and Aspergillus niger; the number of viable bacteria in the preparation is greater than 1×10 10 cfu / g.

9. The multifunctional biological agent according to claim 1, characterized in that: The carrier is one or more of calcium carbonate, eggshell powder, feather powder or shell powder.

10. A method for preparing a multifunctional biological agent according to any one of claims 1 to 9, characterized in that: The steps include: The multifunctional biological preparation can be obtained by uniformly mixing the microbial agent, brown algae oligosaccharide, amphiphilic surfactant, potassium humate and carrier according to a proportion.

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