Breast cancer organoid culture kit

Abstract

The invention relates to the technical field of organoid culture kits, in particular to a breast cancer organoid culture kit which comprises a shell, a supporting plate is fixedly connected to the inner side wall of the shell, a sliding mechanism is arranged in the shell, a liquid adding mechanism is arranged on the supporting plate, and a covering mechanism is arranged at the lower end of the supporting plate. A shaking mechanism is arranged at the end, close to the sliding mechanism, of the upper end of the shell, a limiting mechanism is arranged at the lower end of the shell, a culture solution can be quantitatively added into the culture dish through the arranged structure, and the arranged structure can drive a sliding rod to move downwards while a screw rod moves downwards; a sliding rod moves downwards to drive a culture dish to vibrate, the culture dish vibrates to mix a culture solution in the culture dish, the arranged structure is adopted to drive a suction cup to move downwards while the sliding rod moves downwards, the suction cup moves downwards to firmly adsorb a kit on a supporting surface, and the situation that culture is affected due to shaking of the kit is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of organoid culture kits, in particular to a breast cancer organoid culture kit. Background Art

[0002] The breast cancer organoid culture kit is a chemically defined cell culture medium used to establish and maintain breast cancer organoids. This type of kit has important application prospects in the fields of medical research and precision medicine. The breast cancer organoid culture kit has broad application prospects. It can not only be used for basic research on breast cancer, such as tumor occurrence mechanism, drug screening and efficacy evaluation, but also provide important experimental models and platforms for precision medicine. The breast cancer organoid culture kit contains various reagents and consumables required for breast cancer organoid culture, such as basal culture medium, additives, digestion fluid, freezing fluid and matrix gel, etc. These reagents and consumables have been carefully designed and optimized to ensure the efficient construction and stable maintenance of breast cancer organoids. Through organoid culture, the pathological and genomic characteristics of tumor tissue in vivo can be more accurately simulated, providing strong support for the personalized treatment of breast cancer.

[0003] Although the existing breast cancer organoid culture kit provides an important tool for the research and treatment of breast cancer, it still has some shortcomings. The existing breast cancer organoid culture kit may require a longer culture cycle to achieve the ideal cell number and morphology, which increases the time cost of the research and may affect the timeliness of the experimental results. If the culture solution is not mixed evenly when dripping, some culture reagents may not effectively promote the rapid proliferation of breast cancer cells. Insufficient sealing during the culture process will also affect the breast cancer organoid culture in the kit, resulting in slow growth of the organoid. Therefore, the present invention designs a breast cancer organoid culture kit to solve the above problems. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a breast cancer organoid culture kit, which solves the following technical problems: although the existing breast cancer organoid culture kit provides an important tool for the research and treatment of breast cancer, it still has some shortcomings. The existing breast cancer organoid culture kit may require a longer culture cycle to achieve an ideal cell number and morphology, which increases the time cost of the research and may affect the timeliness of the experimental results. If the culture solution is not mixed evenly when dripping, some culture reagents may not effectively promote the rapid proliferation of breast cancer cells. In addition, insufficient sealing during the culture process will also affect the breast cancer organoid culture in the kit, resulting in a slow growth rate of the organoid.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] The present invention provides a breast cancer organoid culture kit, comprising

[0007] The outer shell has a support plate fixedly connected to its inner side wall, a sliding mechanism is arranged inside the outer shell, a liquid adding mechanism is arranged on the support plate, a covering mechanism is arranged at the lower end of the support plate, a shaking mechanism is arranged at one end of the upper end of the outer shell close to the sliding mechanism, and a limiting mechanism is arranged at the lower end of the outer shell.

[0008] Preferably, a fourth hollow groove is provided inside the support plate, a cover plate is fixedly connected to the lower end of the support plate, a first hollow groove is provided inside the cover plate, the support plate is fixedly connected to the inner side wall of the outer shell, a second hollow groove is also provided inside the support plate, a third hollow groove is also provided inside the support plate, a lens is fixedly connected to the side wall of the first hollow groove inside the support plate, and a container is fixedly connected to the upper end of the support plate.

[0009] Preferably, the sliding mechanism comprises a sliding block, the sliding block is slidably connected to the sliding groove, one end of the sliding block is fixedly connected to a first connecting rod, and the upper end of the sliding block is fixedly connected to a sealing plate.

[0010] Preferably, the sliding mechanism also includes a disc, the upper end of the sealing plate is fixedly connected with a disc, a fifth hollow groove is opened inside the disc, a sixth hollow groove is opened inside the disc, a seventh hollow groove is opened inside the disc, and a culture dish is installed inside the seventh hollow groove.

[0011] Preferably, the liquid adding mechanism comprises a sliding plate, one end of the sliding plate is slidably connected to the second hollow groove, and a communicating groove is provided inside the sliding plate.

[0012] Preferably, the liquid adding mechanism further comprises a first spring, one end of the sliding plate is fixedly connected to the first spring, and one end of the first spring is fixedly connected to the supporting plate.

[0013] Preferably, the covering mechanism comprises a nut, the lower end of the nut is fixedly connected to the support plate, a screw is threadedly engaged inside the nut, and a locking block is fixedly connected to the surface of the screw.

[0014] Preferably, the shaking mechanism includes a sliding rod, the sliding rod is slidably connected to the inside of the fifth hollow groove, and a baffle is fixedly connected to the surface of the sliding rod.

[0015] Preferably, the shaking mechanism also includes a second spring, the lower end of the baffle is fixedly connected to the second spring, the surface of the sliding rod is fitted with a second L-shaped connecting rod, the corner of the second L-shaped connecting rod is fixedly connected to a rotating pin, the rotating pin is elastically connected to the disc through a torsion spring, and the upper end of the second connecting rod is fixedly connected to an arc block.

[0016] Preferably, the limiting mechanism comprises a suction cup, and the upper end of the suction cup is fixedly connected to the sliding rod.

[0017] Beneficial effects of the present invention:

[0018] (1) The breast cancer organoid culture kit described in the present invention can quantitatively add culture fluid to the inside of the culture dish through the set structure. When the screw is rotated downward, the set structure will drive the cover plate to move downward and cover the upper end surface of the disc, so that the three culture dishes placed in the disc can be sealed. At the same time, after the sealing plate slides into the outer shell, the sliding plate will slide into the supporting plate. When the sealing plate slides into the outer shell, it will also be tightly fitted to the side wall surface of the supporting plate.

[0019] (2) The breast cancer organoid culture kit described in the present invention adopts a structure that when the screw moves downward, it also drives the sliding rod to move downward. The downward movement of the sliding rod will in turn drive the culture dish to vibrate, and the vibration of the culture dish will mix the culture solution inside it.

[0020] (3) The breast cancer organoid culture kit described in the present invention has a structure that drives the suction cup to move downward when the sliding rod moves downward. The downward movement of the suction cup will firmly adsorb the kit on the supporting surface, thereby preventing the kit from shaking and affecting the culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0022] Figure 1 A schematic diagram of the overall structure provided by the present invention;

[0023] Figure 2 It is a schematic diagram of the shell structure;

[0024] Figure 3 It is a schematic diagram of the sealing plate connection structure;

[0025] Figure 4 It is a schematic diagram of the disc structure;

[0026] Figure 5 It is a schematic diagram of the connection structure between the support plate and the cover plate;

[0027] Figure 6 is a schematic diagram of the sliding plate structure;

[0028] Figure 7 is a schematic diagram of the lens structure;

[0029] Figure 8 It is a schematic diagram of the connection structure between the screw and the cover plate;

[0030] Fig. 9 It is a schematic diagram of the connection structure between the second connecting rod and the arc block.

[0031] In the figure: 100, housing; 101, slide groove; 200, support plate; 2001, first hollow groove; 2002, second hollow groove; 2003, third hollow groove; 201, cover plate; 2011, fourth hollow groove; 202, lens; 203, container; 300, sliding mechanism; 301, sliding block; 302, first connecting rod; 303, sealing plate; 304, disc; 3041, fifth hollow groove; 3042, sixth hollow groove ; 3043, the seventh hollow groove; 305, the culture dish; 400, the liquid adding mechanism; 401, the sliding plate; 4011, the connecting groove; 402, the first spring; 500, the covering mechanism; 501, the nut; 502, the screw; 503, the locking block; 600, the shaking mechanism; 601, the sliding rod; 602, the baffle; 603, the second spring; 604, the second connecting rod; 605, the arc block; 700, the limiting mechanism; 701, the suction cup. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical means and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The embodiment of the present invention provides a breast cancer organoid culture kit, which solves the technical problem that although the existing breast cancer organoid culture kit provides an important tool for the research and treatment of breast cancer, it still has some shortcomings. The existing breast cancer organoid culture kit may require a longer culture period to achieve the ideal cell number and morphology, which increases the time cost of the research and may affect the timeliness of the experimental results. In addition, if the culture solution is not mixed evenly when dripping, some culture reagents may not effectively promote the rapid proliferation of breast cancer cells. In addition, insufficient sealing during the culture process may also affect the culture of breast cancer organoids in the kit, resulting in slow growth of the organoids.

[0034] The technical solution in the embodiment of the present invention is to solve the above technical problems. The overall idea is as follows: a water drop removal design is adopted inside the screening reagent kit and in the reagent tube placement groove, so that when the reagent tube is used, the water droplets do not affect the reagent, so that the reagent kit cover can be opened for temperature recovery. Compared with the existing reagent kit, in order to avoid the generation of water droplets on the reagent tube wall, the culture fluid can be quantitatively added to the culture dish 305 through the set structure. The set structure drives the sliding rod 601 to move downward while the screw rod 502 moves downward. The sliding rod 601 moves downward and then drives the culture dish 305 to vibrate. The vibration of the culture dish 305 mixes the culture fluid inside it. The set structure drives the suction cup 701 to move downward while the sliding rod 601 moves downward. The suction cup 701 moves downward to firmly adsorb the reagent kit on the support surface, thereby preventing the reagent kit from shaking and affecting the culture.

[0035] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0036] like Figures 1 to 9 As shown, an embodiment of the present invention provides a breast cancer organoid culture kit, comprising

[0037] The shell 100 has a support plate 200 fixedly connected to its inner side wall, and is characterized in that: a sliding mechanism 300 is arranged inside the shell 100, a liquid adding mechanism 400 is arranged on the support plate 200, a covering mechanism 500 is arranged at the lower end of the support plate 200, a shaking mechanism 600 is arranged at one end of the upper end of the shell 100 close to the sliding mechanism 300, and a limiting mechanism 700 is arranged at the lower end of the shell 100; the culture solution can be quantitatively added to the culture dish 305 by the arranged structure, and the arranged structure can drive the sliding rod 601 to move downward while the screw rod 502 moves downward, and the sliding rod 601 moves downward and then drives the culture dish 305 to vibrate, and the vibration of the culture dish 305 mixes the culture solution inside it, and the arranged structure can drive the suction cup 701 to move downward while the sliding rod 601 moves downward, and the downward movement of the suction cup 701 can firmly adsorb the reagent box on the support surface to prevent the reagent box from affecting the culture due to shaking.

[0038] Preferably, a fourth hollow groove 2001 is opened inside the support plate 200, a cover plate 201 is fixedly connected to the lower end of the support plate 200, a first hollow groove 2011 is opened inside the cover plate 201, the support plate 200 is fixedly connected to the inner side wall of the outer shell 100, a second hollow groove 2002 is also opened inside the support plate 200, a third hollow groove 2003 is also opened inside the support plate 200, a lens 202 is fixedly connected to the side wall of the first hollow groove 2001 inside the support plate 200, and a container 203 is fixedly connected to the upper end of the support plate 200.

[0039] Preferably, the sliding mechanism 300 includes a sliding block 301, the sliding block 301 is slidably connected to the sliding groove 101, one end of the sliding block 301 is fixedly connected to the first connecting rod 302, the upper end of the sliding block 301 is fixedly connected to the sealing plate 303, the upper end of the sealing plate 303 is fixedly connected to the disc 304, the disc 304 has a fifth hollow groove 3041, the disc 304 also has a sixth hollow groove 3042, the disc 304 has a seventh hollow groove 3043, and the seventh hollow groove 3043 contains a culture dish 305; firstly, the first connecting rod 302 is pulled outward to move, the first connecting rod 302 moves outward, the sliding block 301 moves outward, and the outward movement of the sliding block 301 drives the sealing plate 303 to move outward. The outward movement of the sealing plate 303 will drive the disc 304 to move outward, and the outward movement of the disc 304 will drive the culture dish 305 to move outward. The structure set up will drive the sealing plate 303 away from the sliding plate 401 when pulling the first connecting rod 302 to move outward. At this time, the first spring 402 will be reset and drive the sliding plate 401 to move outward. The outward movement of the sliding plate 401 will drive the connecting groove 4011 to coincide with the lower opening of the third hollow groove 2003. At this time, the culture fluid inside the third hollow groove 2003 will enter the connecting groove 4011. When the sliding plate 401 slides into the second hollow groove 2002, a certain amount of culture fluid inside the connecting groove 4011 will be added to the corresponding culture dish 305. The structure set up can quantitatively add culture fluid to the inside of the culture dish 305.

[0040] Preferably, the liquid adding mechanism 400 includes a sliding plate 401, one end of the sliding plate 401 is slidably connected to the second hollow groove 2002, a connecting groove 4011 is opened inside the sliding plate 401, one end of the sliding plate 401 is fixedly connected to the first spring 402, and one end of the first spring 402 is fixedly connected to the support plate 200.

[0041] Preferably, the covering mechanism 500 includes a nut 501, the lower end of the nut 501 is fixedly connected to the support plate 200, the internal thread of the nut 501 is meshed with a screw 502, and the surface of the screw 502 is fixedly connected with a locking block 503; at this time, the screw 502 is rotated, and the rotation of the screw 502 will drive the cover plate 201 to move downward, and the downward movement of the cover plate 201 will insert the screw 502 into the fifth hollow groove 3041, and the downward movement of the cover plate 201 will cover the upper surface of the disc 304, so that the three culture dishes 305 placed in the disc 304 can be sealed, and at the same time, the sealing plate 303 is moved toward the outer shell 10 0, the sliding plate 401 will slide into the supporting plate 200, and when the sealing plate 303 slides into the outer shell 100, it will also be closely fitted to the side wall surface of the supporting plate 200. The structure is set so that when the screw 502 moves downward, the cover plate 201 will be driven to move downward and cover the upper end surface of the disc 304, so that the three culture dishes 305 placed in the disc 304 can be sealed. At the same time, after the sealing plate 303 slides into the outer shell 100, the sliding plate 401 will slide into the supporting plate 200, and when the sealing plate 303 slides into the outer shell 100, it will also be closely fitted to the side wall surface of the supporting plate 200.

[0042] Preferably, the shaking mechanism 600 includes a sliding rod 601, the sliding rod 601 is slidably connected to the inside of the fifth hollow groove 3041, a baffle 602 is fixedly connected to the surface of the sliding rod 601, a second spring 603 is fixedly connected to the lower end of the baffle 602, an L-shaped second connecting rod 604 is attached to the surface of the sliding rod 601, a rotating pin is fixedly connected at the corner of the L-shaped second connecting rod 604, the rotating pin is elastically connected to the disc 304 through a torsion spring, and an arc block 605 is fixedly connected to the upper end of the second connecting rod 604; when the screw rod 502 moves downward, it will also drive the sliding rod 601 to move downward, and the downward movement of the sliding rod 601 will drive the baffle 60 2 moves downward, the baffle 602 moves downward to compress the second spring 603, and the sliding rod 601 is provided with continuous annular protrusions on its surface, so that when the sliding rod 601 moves downward, the annular protrusions on its surface will drive the second connecting rod 604 to vibrate, and the vibration of the second connecting rod 604 will transmit the vibration to the arc block 605, and the vibration of the arc block 605 will drive the culture dish 305 to vibrate, and the vibration of the culture dish 305 will mix the culture solution inside it. With the structure set, the screw rod 502 will also drive the sliding rod 601 to move downward at the same time as the downward movement, and the sliding rod 601 will further drive the culture dish 305 to vibrate, and the vibration of the culture dish 305 will mix the culture solution inside it.

[0043] Preferably, the limiting mechanism 700 includes a suction cup 701, the upper end of which is fixedly connected to the sliding rod 601. When the sliding rod 601 moves downward, the suction cup 701 is also driven to move downward. The downward movement of the suction cup 701 will firmly adsorb the reagent box on the supporting surface, thereby preventing the reagent box from shaking and affecting the culture. The structure is set up so that when the sliding rod 601 moves downward, the suction cup 701 is also driven to move downward. The downward movement of the suction cup 701 will firmly adsorb the reagent box on the supporting surface, thereby preventing the reagent box from shaking and affecting the culture.

[0044] Working principle: When the present invention is used, the first connecting rod 302 is first pulled outward to move, the first connecting rod 302 moves outward, the sliding block 301 moves outward, the sliding block 301 moves outward, the sealing plate 303 moves outward, the sealing plate 303 moves outward, the disc 304 moves outward, the disc 304 moves outward, and the culture dish 305 moves outward. The structure is set to pull the first connecting rod 302 outward to drive the sealing plate 303 away from the sliding plate 401. At this time, the first spring 402 will reset and then drive the sliding plate 401 to move outward. The sliding plate 401 moves outward to drive the connecting groove 4011 and the third air The lower end openings of the center groove 2003 overlap, and at this time, the culture fluid inside the third hollow groove 2003 will enter the connecting groove 4011, and when the sliding plate 401 slides into the second hollow groove 2002, a certain amount of culture fluid inside the connecting groove 4011 will be added into the corresponding culture dish 305. A lens 202 is installed inside the first hollow groove 2001, and the lens 202 is directly above the culture dish 305. In this way, when the culture dish 305 is sealed, the culture status of the breast cancer organoids in the culture dish 305 can be observed through the lens 202, and the culture fluid can be quantitatively added to the culture dish 305 through the set structure.

[0045] At this time, the screw rod 502 is rotated, and the rotation of the screw rod 502 drives the cover plate 201 to move downward. The downward movement of the cover plate 201 will insert the screw rod 502 into the fifth hollow groove 3041, and the downward movement of the cover plate 201 will cover the upper surface of the disc 304, so that the three culture dishes 305 placed in the disc 304 can be sealed. At the same time, after the sealing plate 303 slides into the housing 100, the sliding plate 401 slides into the supporting plate 200, and after the sealing plate 303 slides into the housing 100, the sliding plate 401 slides into the supporting plate 200. When the sealing plate 303 slides into the outer shell 100, the sliding plate 401 will slide into the support plate 200. When the sealing plate 303 slides into the outer shell 100, it will also fit closely to the side wall surface of the support plate 200. When the sealing plate 303 slides into the outer shell 100, it will also fit closely to the side wall surface of the support plate 200.

[0046] When the screw rod 502 moves downward, it will also drive the sliding rod 601 to move downward, and the downward movement of the sliding rod 601 will drive the baffle 602 to move downward. The downward movement of the baffle 602 will compress the second spring 603. The surface of the sliding rod 601 is provided with continuous annular protrusions, so that when the sliding rod 601 moves downward, the annular protrusions on its surface will drive the second connecting rod 604 to vibrate, and the vibration of the second connecting rod 604 will transmit the vibration to the arc block 605. The vibration of the arc block 605 will drive the culture dish 305 to vibrate, and the vibration of the culture dish 305 will mix the culture solution inside it. With the structure set, when the screw rod 502 moves downward, it will also drive the sliding rod 601 to move downward, and the downward movement of the sliding rod 601 will drive the culture dish 305 to vibrate, and the vibration of the culture dish 305 will mix the culture solution inside it.

[0047] When the sliding rod 601 moves downward, the suction cup 701 will also move downward. The downward movement of the suction cup 701 will firmly adsorb the test kit on the supporting surface, thereby preventing the test kit from shaking and affecting the culture. The structure is set up so that when the sliding rod 601 moves downward, the suction cup 701 will also move downward. The downward movement of the suction cup 701 will firmly adsorb the test kit on the supporting surface, thereby preventing the test kit from shaking and affecting the culture.

[0048] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A breast cancer organoid culture kit, comprising a housing (100), wherein a support plate (200) is fixedly connected to an inner side wall of the housing (100), characterized in that: A sliding mechanism (300) is arranged inside the shell (100), a liquid adding mechanism (400) is arranged on the support plate (200), a covering mechanism (500) is arranged at the lower end of the support plate (200), a shaking mechanism (600) is arranged at one end of the upper end of the shell (100) close to the sliding mechanism (300), and a limiting mechanism (700) is arranged at the lower end of the shell (100).

2. A breast cancer organoid culture kit according to claim 1, characterized in that: A fourth hollow groove (2001) is provided inside the support plate (200); a cover plate (201) is fixedly connected to the lower end of the support plate (200); a first hollow groove (2011) is provided inside the cover plate (201); the support plate (200) is fixedly connected to the inner side wall of the housing (100); a second hollow groove (2002) is also provided inside the support plate (200); a third hollow groove (2003) is also provided inside the support plate (200); a lens (202) is fixedly connected to the side wall of the first hollow groove (2001) inside the support plate (200); and a container (203) is fixedly connected to the upper end of the support plate (200).

3. A breast cancer organoid culture kit according to claim 1, characterized in that: The sliding mechanism (300) comprises a sliding block (301), the sliding block (301) is slidably connected to the sliding groove (101), one end of the sliding block (301) is fixedly connected to a first connecting rod (302), and the upper end of the sliding block (301) is fixedly connected to a sealing plate (303).

4. A breast cancer organoid culture kit according to claim 3, characterized in that: The sliding mechanism (300) further comprises a disc (304), the upper end of the sealing plate (303) is fixedly connected with the disc (304), a fifth hollow groove (3041) is provided inside the disc (304), a sixth hollow groove (3042) is provided inside the disc (304), a seventh hollow groove (3043) is provided inside the disc (304), and a culture dish (305) is arranged inside the seventh hollow groove (3043).

5. A breast cancer organoid culture kit according to claim 2, characterized in that: The liquid adding mechanism (400) comprises a sliding plate (401), one end of the sliding plate (401) is slidably connected to the second hollow groove (2002), and a connecting groove (4011) is provided inside the sliding plate (401).

6. A breast cancer organoid culture kit according to claim 5, characterized in that: The liquid adding mechanism (400) further comprises a first spring (402), one end of the sliding plate (401) is fixedly connected to the first spring (402), and one end of the first spring (402) is fixedly connected to the support plate (200).

7. A breast cancer organoid culture kit according to claim 2, characterized in that: The covering mechanism (500) comprises a nut (501), the lower end of the nut (501) is fixedly connected to the support plate (200), the internal thread of the nut (501) is meshed with a screw rod (502), and the surface of the screw rod (502) is fixedly connected with a locking block (503).

8. A breast cancer organoid culture kit according to claim 4, characterized in that: The shaking mechanism (600) comprises a sliding rod (601), the sliding rod (601) is slidably connected to the inside of the fifth hollow groove (3041), and a baffle (602) is fixedly connected to the surface of the sliding rod (601).

9. A breast cancer organoid culture kit according to claim 8, characterized in that: The shaking mechanism (600) also includes a second spring (603), the lower end of the baffle (602) is fixedly connected to the second spring (603), the surface of the sliding rod (601) is fitted with an L-shaped second connecting rod (604), a rotating pin is fixedly connected at the corner of the L-shaped second connecting rod (604), the rotating pin is elastically connected to the disk (304) through a torsion spring, and the upper end of the second connecting rod (604) is fixedly connected to an arc block (605).

10. The breast cancer organoid culture kit according to claim 8, characterized in that: The limiting mechanism (700) comprises a suction cup (701), and the upper end of the suction cup (701) is fixedly connected to the sliding rod (601).

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