A carrageenan gelling composition and its preparation method and application

Abstract

The present invention discloses a gelling composition of carrageenan and its preparation method and application, wherein the preparation method includes: S1: mixing yeast β-glucan, carrageenan and deionized water for high temperature and high pressure homogenization reaction to obtain mixture A; S2: cooling mixture A to room temperature; adding tea seed oil, rehmannia root, allantoin and dipotassium glycyrrhizate for low temperature and high pressure homogenization to obtain mixture B; S3: using a pH regulator to adjust the pH value of mixture B to 5-6; and adding a preservative to obtain a gelling composition. The preparation method of the gelling composition of carrageenan, through high temperature and high pressure homogenization reaction to form a gelling composition of yeast β-glucan and carrageenan, and then through low temperature and high pressure homogenization to make each efficacy uniformly dispersed in the gelling composition, improve the dispersion uniformity of the efficacy components in the gelling composition, and at the same time, the form of the gelling composition can form an effective protective layer on the skin surface, thereby making the effect of each efficacy component lasting and efficient.

Description

Technical Field

[0001] The present invention relates to the technical field of gelling compositions, and in particular to a carrageenan gelling composition and a preparation method and application thereof. Background Art

[0002] Burns often occur frequently in daily life and work. Severe burns require medical surgery, while minor burns can be relieved by ointments or Chinese herbal compositions. In the prior art, burn ointments are all special components formed by using Chinese herbal compositions.

[0003] CN117503860A discloses a scald plaster, which is composed of a mercurochrome composition and a medicine surface, and the mercurochrome composition and the medicine surface are used separately. The mercurochrome composition comprises, by weight, 3-9 parts of sulfa crystals, 20-40 parts of mercurochrome, and 4-10 parts of zinc oxide; and the medicine surface comprises, by weight, 4-10 parts of artemisia annua, 3-9 parts of cactus, 10-20 parts of aloe bark, 6-12 parts of white insect wax, 5-15 parts of beeswax, 8-20 parts of ascidiana, 4-10 parts of jianglongcao, 4-10 parts of borneol, 8-20 parts of honeysuckle, 4-10 parts of peony bark, and 3-9 parts of dandelion. Among them, the mercurochrome composition has antibacterial, anti-inflammatory and anti-infective effects; Artemisia argyi has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and clearing rashes and removing spots; Cactus has antibacterial, anti-inflammatory, anti-irritation and repairing effects, and has good stability and skin compatibility; Aloe vera peel has the effects of promoting blood circulation, reducing swelling, promoting tissue regeneration and relieving pain; Ascites has the effects of reconciling the exterior and interior, relieving liver depression, and raising yang energy; Dragon Grass can increase granulation, tissue structure and epidermal cell regeneration, repair skin appendages and has a strong wound healing effect; Honeysuckle reduces swelling and relieves pain, and is used for mild burns caused by water or fire to relieve skin swelling and pain; Paeony Bark has an inhibitory effect on a variety of bacteria and viruses, and effectively prevents wound infection; Dandelion has the effects of clearing heat and relieving pain, promoting tissue regeneration, promoting blood circulation and unblocking meridians, and relieving swelling and pain in sores and ulcers. However, during the preparation process of this traditional Chinese medicine composition, various mixed powders are added to white insect wax and beeswax, and after sufficient mixing, they are added to a stirring kettle and heated and stirred to obtain a finished plaster. This stirring method can only physically combine the various compositions together. During the plaster packaging process, the various compositions may be unevenly distributed, thereby affecting the medicinal properties of the plaster.

[0004] The existing preparation method for burn ointment cannot ensure that the various active ingredients are evenly and effectively distributed, and cannot maximize the efficacy of the burn ointment. Summary of the Invention

[0005] The present invention aims to provide a method for preparing a gelled composition of carrageenan, wherein a gelled composition of yeast β-glucan and carrageenan is formed by a high-temperature and high-pressure homogenization reaction, and then the various functional ingredients are uniformly dispersed in the gelled composition by low-temperature and high-pressure homogenization, thereby improving the dispersion uniformity of the functional ingredients in the gelled composition. At the same time, the gelled composition can form an effective protective layer on the skin surface, thereby ensuring that the effects of the various functional ingredients are long-lasting and highly effective.

[0006] To achieve the above object, a method for preparing a carrageenan gel composition is provided, comprising:

[0007] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain mixture A;

[0008] S2: Cooling the mixture A to room temperature; adding tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate and homogenizing under low temperature and high pressure to obtain a mixture B;

[0009] S3: adjusting the pH value of the mixture B to 5-6 with a pH adjuster; and adding a preservative to obtain a gelled composition.

[0010] Furthermore, the homogenization pressure of the high temperature and high pressure homogenization is 8-10 MPa, and the homogenization temperature is 50-70°C.

[0011] Furthermore, the pressure of the low-temperature and high-pressure homogenization is 6-8 MPa, and the homogenization temperature is 20-25°C.

[0012] Furthermore, the mass ratio of the camellia oil, rehmannia root, allantoin and dipotassium glycyrrhizate is 1:1:2:2.

[0013] Furthermore, the mass ratio of the sum of the mass of the camellia oil, rehmannia root, allantoin and dipotassium glycyrrhizate to the mixture A is 1:10.

[0014] Furthermore, the mass ratio of the yeast β-glucan to carrageenan is 5:1.

[0015] Furthermore, the preservative includes at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and butyl parahydroxybenzoate.

[0016] Furthermore, the pH regulator is sodium citrate.

[0017] A carrageenan gelling composition is prepared based on the above-mentioned preparation method.

[0018] The carrageenan gel composition is used to prepare burn ointment.

[0019] Beneficial effects of the present invention:

[0020] (1) The present invention forms a gelled composition of yeast β-glucan and carrageenan through a high-temperature and high-pressure homogenization reaction, forming the main scaffold component of the composition of the present application, wherein yeast β-glucan does not affect the gelling effect of carrageenan, and the two undergo a gelling reaction during the high-temperature and high-pressure homogenization process to form an interlaced network structure. The finally formed network gelled scaffold can serve as the main scaffold for carrying its effective ingredients, and can also serve as an outer protective film of the skin to cover and protect the effective ingredients and the burn site during use.

[0021] (2) The present invention also adds tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate as effective ingredients, wherein tea seed oil and rehmannia root can play a role in healing burns, and allantoin and dipotassium glycyrrhizate have good anti-inflammatory effects. Tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate are uniformly dispersed in the mesh gel scaffold under low temperature and high pressure homogenization conditions, so that the effective ingredients can be uniformly dispersed in the carrier. At the same time, the low temperature environment can also ensure that the biological activity and efficacy of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate are not destroyed, ensuring that they can fully exert their efficacy during use. DETAILED DESCRIPTION

[0022] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention are within the scope of protection of the present invention. Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the examples of this application are commercially available; unless specifically specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.

[0023] The present application provides a method for preparing a carrageenan gel composition, comprising:

[0024] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization to obtain a mixture A. The homogenization pressure of the high-temperature and high-pressure homogenization is 8-10 MPa and the homogenization temperature is 50-70° C. The mass ratio of the yeast β-glucan to carrageenan is 5:1.

[0025] S2: Cool mixture A to room temperature; add tea seed oil, Rehmannia root, allantoin, and dipotassium glycyrrhizate and perform low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 6-8 MPa, and the homogenization temperature is 20-25°C. The mass ratio of the tea seed oil, Rehmannia root, allantoin, and dipotassium glycyrrhizate is 1:1:2:2. The mass ratio of the sum of the mass of the tea seed oil, Rehmannia root, allantoin, and dipotassium glycyrrhizate to the mass of mixture A is 1:10.

[0026] S3: Adjusting the pH of mixture B to 5-6 using a pH adjuster; and adding a preservative to obtain a gel composition. The preservative comprises at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and butyl parahydroxybenzoate. The pH adjuster is sodium citrate.

[0027] The present invention uses a high-temperature, high-pressure homogenization reaction to form a gelled composition with yeast β-glucan and carrageenan, forming the main scaffold component of the composition of the present application. Yeast β-glucan does not affect the gelling effect of carrageenan, and the two undergo a gelling reaction during the high-temperature, high-pressure homogenization process, forming an interlaced network structure. The resulting network gelled scaffold can serve as the main scaffold for its active ingredients and can also serve as an outer protective film on the skin to cover and protect the active ingredients and burn sites during use. The homogenization pressure of the high-temperature, high-pressure homogenization is 8-10MPa, and the homogenization temperature is 50-70°C. Under these conditions, yeast β-glucan and carrageenan can undergo a gelling reaction well to form a network gelled scaffold.

[0028] The present invention further incorporates tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate as effective ingredients, wherein tea seed oil and rehmannia root can play a role in healing burns, and allantoin and dipotassium glycyrrhizate have good anti-inflammatory effects. The tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate are uniformly dispersed in a mesh gel scaffold under low-temperature and high-pressure homogenization conditions, allowing the effective ingredients to be uniformly dispersed in the carrier. Simultaneously, the low-temperature environment can also ensure that the biological activities and effects of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate are not destroyed, ensuring that they fully exert their effects during use. The low-temperature and high-pressure homogenization pressure is 6-8 MPa, and the homogenization temperature is 20-25° C. Under these conditions, the biological activities and effects of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate are not destroyed, and under high-pressure homogenization, they can be uniformly dispersed in the mesh gel scaffold.

[0029] The gel composition prepared in the present application can be used as a burn ointment. During use as a burn ointment, the mesh gel scaffold can cover the burned skin, allowing the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate embedded in the mesh gel scaffold to fully exert their effects, relieve burns, and enhance skin repair ability.

[0030] Example 1

[0031] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0032] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high-temperature and high-pressure homogenization is 8 MPa, the homogenization temperature is 50° C., and the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0033] S2: Cool mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate and perform low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 6 MPa and the homogenization temperature is 20°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to the mass of mixture A is 1:10.

[0034] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition. The gel composition is used as a burn ointment.

[0035] Example 2

[0036] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0037] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high-temperature and high-pressure homogenization is 10 MPa, and the homogenization temperature is 70° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0038] S2: Cool mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate and perform low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 8 MPa and the homogenization temperature is 25°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate is 1:1:2:2; and the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to the mass of mixture A is 1:10.

[0039] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition. The gel composition is used as a burn ointment.

[0040] Example 3

[0041] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0042] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high-temperature and high-pressure homogenization is 9 MPa, the homogenization temperature is 60° C., and the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0043] S2: Cool mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate and perform low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 7 MPa and the homogenization temperature is 22°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to the mass of mixture A is 1:10.

[0044] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition. The gel composition is used as a burn ointment.

[0045] Comparative Example 1

[0046] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0047] S1: Yeast β-glucan, carrageenan, deionized water, tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate were mixed and subjected to high-temperature and high-pressure homogenization. The homogenization pressure was 9 MPa and the homogenization temperature was 60°C. The mass ratio of yeast β-glucan to carrageenan was 5:1. The mass ratio of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate was 1:1:2:2. The mass ratio of the sum of the mass of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to mixture A was 1:10.

[0048] S2: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition, which is used as a burn ointment.

[0049] Comparative Example 2

[0050] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0051] S1: Yeast β-glucan, carrageenan, deionized water, tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate were mixed and subjected to low-temperature, high-pressure homogenization at a pressure of 7 MPa and a temperature of 22°C. The mass ratio of yeast β-glucan to carrageenan was 5:1. The mass ratio of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate was 1:1:2:2. The mass ratio of the sum of the mass of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to mixture A was 1:10.

[0052] S2: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition, which is used as a burn ointment.

[0053] Comparative Example 3

[0054] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0055] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high-temperature and high-pressure homogenization is 9 MPa, the homogenization temperature is 60° C., and the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0056] S2: Cool mixture A to room temperature; add tea seed oil and Rehmannia root and perform low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 7 MPa and the homogenization temperature is 22°C. The mass ratio of the tea seed oil to Rehmannia root is 1:1; the mass ratio of the combined mass of the tea seed oil and Rehmannia root to mixture A is 1:10.

[0057] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition. The gel composition is used as a burn ointment.

[0058] Comparative Example 4

[0059] This embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0060] S1: Yeast β-glucan, carrageenan, and deionized water are mixed and subjected to high-temperature and high-pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high-temperature and high-pressure homogenization is 9 MPa, the homogenization temperature is 60° C., and the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0061] S2: Cooling mixture A to room temperature; adding allantoin and dipotassium glycyrrhizate and performing low-temperature, high-pressure homogenization to obtain mixture B. The low-temperature, high-pressure homogenization pressure is 7 MPa, and the homogenization temperature is 22°C. The mass ratio of the allantoin to dipotassium glycyrrhizate is 1:1; and the mass ratio of the sum of the mass of the allantoin and dipotassium glycyrrhizate to the mass of mixture A is 1:10.

[0062] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl parahydroxybenzoate to obtain a gel composition. The gel composition is used as a burn ointment.

[0063] Experimental example

[0064] 1. Establishment of Mouse Scald Model

[0065] 24 hours before the experiment, mice were depilated by applying an appropriate amount of 5% sodium sulfide solution to the backs of the mice. The hairless area on the backs of the mice was approximately 2 x 2 cm. To establish the burn model, a 50g weight was placed in boiling water for 10 minutes, quickly removed, and firmly adhered to the smooth, hairless skin of the mice for 8 seconds to create a deep second-degree burn model. A blank control group received no treatment.

[0066] 2. Skin healing rate

[0067] Fifty mice were divided equally into five groups and treated with the burn ointments described in Examples 1-3 and Comparative Examples 1-4, respectively. 5% sodium sulfide was applied to the backs of the mice to remove hair, covering an area of 2 x 2 cm. The dorsal skin of the mice was placed firmly against a weight in boiling distilled water. The burns were timed with a stopwatch for 8 seconds in each group, completing the burn model.

[0068] After the mouse scald model was established, 0.1 g of scald ointment was applied to the wound skin once daily, and the wound healing rate was calculated. The results are shown in Table 1.

[0069] On the 14th day after the model was completed, the sulfuric acid paper marking method was used to replace the area with the mass of the marked area, and the average wound healing rate was calculated. The specific calculation formula is as follows:

[0070] Wound healing rate = (original scald area - unhealed area) / original scald area × 100%

[0071] Criteria for judging wound healing: the scab on the wound falls off, new epidermis is generated, and small hairs appear.

[0072] Table 1: Mouse skin healing rates in various examples and comparative examples

[0073]

[0074] It can be seen that: under the same components, only high-temperature and high-pressure homogenization or low-temperature and high-pressure homogenization cannot fully exert the effect of the effective ingredients, and the mouse skin healing rate is greater than that of the blank control group, but is much lower than the mouse skin healing rate corresponding to the burn ointment in Examples 1 to 3.

[0075] Under the same process conditions, compared with the comparative examples containing only camellia oil and rehmannia, or only allantoin and dipotassium glycyrrhizate, the effective ingredients of Examples 1 to 3 of the present application, which simultaneously contain camellia oil, rehmannia, allantoin and dipotassium glycyrrhizate, can significantly improve the skin healing rate of mice.

[0076] It is understandable that the above embodiments only express the preferred implementation modes of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the patent scope of the present invention. It should be pointed out that for ordinary technicians in this field, without departing from the concept of the present invention, the above technical features can be freely combined, and several deformations and improvements can be made, all of which fall within the scope of protection of the present invention. Therefore, all equivalent changes and modifications made to the scope of the claims of the present invention should fall within the scope of coverage of the claims of the present invention.

Claims

1. A method for preparing a carrageenan gel composition, characterized in that: include: S1: Mixing yeast β-glucan, carrageenan, and deionized water and subjecting them to a high-temperature, high-pressure homogenization reaction to obtain a mixture A; the high-temperature, high-pressure homogenization has a homogenization pressure of 8-10 MPa and a homogenization temperature of 50-70°C; S2: Cooling the mixture A to room temperature; adding tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate and homogenizing at low temperature and high pressure to obtain a mixture B; the pressure of the low temperature and high pressure homogenization is 6-8 MPa, and the homogenization temperature is 20-25°C; S3: Using a pH regulator to adjust the pH value of the mixture B to 5-6; and adding a preservative to obtain a gelling composition; the mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizate to the mass of the mixture A is 1:10; the mass ratio of the yeast β-glucan to carrageenan is 5:

1.

2. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The preservative includes at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and butyl parahydroxybenzoate.

3. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The pH regulator is sodium citrate.

4. A carrageenan gelling composition, characterized in that: It is prepared based on the preparation method according to any one of claims 1 to 3.

5. Use of the carrageenan gel composition according to claim 4 in preparing a burn ointment.

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