Rice seedling root length non-injury rapid detection salt tolerance identification device

Abstract

The present application relates to the technical field of rice salt tolerance identification, and discloses a rice seedling stage root length non-damage rapid detection salt tolerance identification device, which comprises an identification tank, the front of the identification tank is a transparent observation window, a culture floating plate is movably arranged in the middle of the identification tank, culture openings are uniformly arranged in the middle of the culture floating plate, culture nets are fixedly installed at the bottom of the culture openings, support bases are fixedly connected to the two sides of the bottom of the identification tank, and movable tracks are fixedly connected to the front and back of the middle of the support bases. The movable supporting plate is additionally arranged at the bottom of the identification tank, and the rotating shaft magnetic strip on the movable supporting plate drives the rotation of the rotor in the identification tank to form a water vortex, so that a downward pulling force is formed on the center line of the vortex, the rice root system at the bottom of the culture opening is gathered and stretched, and the experimenter can directly observe the growth length of the rice seedling root system, thereby avoiding the complicated operation of root length measurement and damage to the rice seedling root system.

Description

TECHNICAL FIELD

[0001] The application relates to a salt-tolerant rice identification technology field, in particular to a salt-tolerant rice identification device for rapid detection of root length without damage in the seedling stage of rice. BACKGROUND

[0002] The rice salt-tolerance detection technology refers to a series of technologies and methods for evaluating the tolerance of rice varieties or germplasm to salt stress. These technologies aim to help agricultural researchers and breeders to select rice varieties with stronger adaptability to saline-alkali environments, so as to improve the yield and quality of rice. The existing rice salt-tolerance detection is usually based on the final growth status of rice, ignoring the growth indicators of rice in the seedling stage. The growth indicators of rice in the seedling stage can better reflect the salt tolerance of rice, and the root length of rice is the most characteristic growth indicator of rice.

[0003] In the salt-tolerance identification experiment of rice seedlings, the detection method commonly used is to cut the root system of the rice seedlings after being planted for measurement of the growth amount. This method causes irreversible damage to the test rice, making it difficult to detect the growth indicators of the next growth cycle of the rice, and thus it is difficult to obtain the continuous root growth data of the rice seedlings in multiple time periods. In addition, the culture solution in the incubator is not uniform in precipitation during a long cultivation process, which affects the accuracy of the test data. SUMMARY

[0004] In view of the deficiencies of the existing rice seedling salt-tolerance test identification device in the background art.

[0005] The application provides the following technical scheme: a rice seedling root length non-damage rapid detection salt tolerance identification device, which comprises an identification tank, the front surface of the identification tank is a transparent observation window, a culture floating plate is movably arranged in the middle of the identification tank, culture openings are uniformly arranged in the middle of the culture floating plate, culture nets are fixedly installed at the bottom of the culture openings, support bases are fixedly connected to the two sides of the bottom of the identification tank, movable rails are fixedly connected to the front surface and the back surface of the middle of the support bases, a sliding groove is arranged in the middle of the movable rail, a movable supporting plate is movably connected to the sliding groove, the sliding groove on the movable rail on one side of the front surface of the support base penetrates the movable rail on the same side, one end of the front surface of the movable supporting plate penetrates the movable rail through the sliding groove to the outside of the movable rail, a movable knob is fixedly connected to the front surface of the movable supporting plate, the sliding groove on the movable rail on one side of the back surface of the support base is located on the inside of the movable rail and does not penetrate the movable rail, a positioning hole is arranged in the sliding groove at a position corresponding to the center of the culture opening, a motor is fixedly installed on the top of the movable supporting plate, a rotating shaft magnetic strip is fixedly connected to the rotating shaft of the motor in the horizontal direction, a rotor is movably arranged on the bottom surface in the identification tank, a main rotating cylinder is fixedly connected to the bottom of the culture floating plate, a water return groove is arranged at the connection position between the top of the main rotating cylinder and the bottom surface of the culture floating plate, a first auxiliary rotating cylinder is movably sleeved in the main rotating cylinder, and a second auxiliary rotating cylinder is movably sleeved in the first auxiliary rotating cylinder.

[0006] Preferably, the culture floating plate floats with the liquid level in the identification tank, so that the culture net is always in contact with the liquid level in the identification tank.

[0007] Preferably, the back surface of the movable supporting plate is fixedly connected with a protruding clamping shaft, the diameter of the clamping shaft is smaller than the inner diameter of the positioning hole, and the positioning hole is arranged at a position consistent with the culture opening.

[0008] Preferably, the main rotating cylinder is coaxial with the culture opening.

[0009] Preferably, the first auxiliary rotating cylinder can move up and down along the main rotating cylinder, a limiting ring is arranged at the top of the first auxiliary rotating cylinder to prevent the first auxiliary rotating cylinder from being separated from the inside of the main rotating cylinder, the second auxiliary rotating cylinder can move up and down along the first auxiliary rotating cylinder, and a limiting ring is arranged at the top of the second auxiliary rotating cylinder to prevent the second auxiliary rotating cylinder from being separated from the inside of the first auxiliary rotating cylinder.

[0010] Preferably, thread grooves are arranged in the inner walls of the main rotating cylinder and the first auxiliary rotating cylinder, rotating shafts are arranged on the two sides of the top of the first auxiliary rotating cylinder and the second auxiliary rotating cylinder, the rotating shaft at the top end of the second auxiliary rotating cylinder is movably connected with the thread groove in the inner wall of the first auxiliary rotating cylinder, the rotating shaft at the top end of the first auxiliary rotating cylinder is movably connected with the thread groove in the inner wall of the main rotating cylinder, a disc spring is fixedly installed in the middle of the main rotating cylinder and the first auxiliary rotating cylinder, a disc spring is fixedly installed in the middle of the first auxiliary rotating cylinder and the second auxiliary rotating cylinder, and a fan blade is fixedly installed at the bottom of the middle of the second auxiliary rotating cylinder.

[0011] Preferably, the disc spring is in a force-free state, and the first and second auxiliary cylinders are pulled up and shrunk into the main cylinder.

[0012] The present application has the following advantages:

[0013] 1、The present application is characterized in that the moving supporting plate is installed at the bottom of the identification pool, and the rotating shaft magnetic strip on the moving supporting plate drives the rotation of the rotor in the identification pool to form a water vortex, so that a downward pulling force is formed on the center line of the water vortex, thereby facilitating the gathering and stretching of the rice root system at the bottom of the culture opening, and the experimenter can directly observe the growth length of the rice seedling root system without the need to remove the seedling from the culture floating plate for measurement, thereby avoiding the cumbersome operation of measuring the root length and the damage to the rice seedling root system.

[0014] 2、The present application is characterized in that the main cylinder coaxial with the culture opening is arranged below the culture floating plate, and the main cylinder is used to form a ring barrier for the water vortex, thereby avoiding the involvement of the root system under the adjacent culture opening in the measurement of the root length under a certain culture opening, and further ensuring the protection of the root system during the measurement process, so that the rice seedling root system after measurement is not affected, and the continuous root growth data of the rice seedling in multiple time periods can be easily obtained.

[0015] 3、The present application is characterized in that the three-stage cylinder is arranged to avoid the length fixation of the single main cylinder, limit the floating height of the culture floating plate, avoid the problem that the culture floating plate is lifted by the main cylinder when the water level in the identification pool is low, and cause the culture net to be separated from the water surface, and the disc spring and the screw groove in the three-stage cylinder are used to make the first and second auxiliary cylinders shrink into the main cylinder in the normal state, so as not to limit and affect the free growth of the rice seedling root system.

[0016] 4、The present application is characterized in that the water vortex formed by the main cylinder and the rotor cooperates with each other, the culture solution in the main cylinder generates a water flow downward along the center line, the culture solution is thrown out from the bottom of the second auxiliary cylinder, and the culture solution is supplemented into the water return groove above the main cylinder, so as to mix the culture solution in the identification pool on the upper and lower layers, avoid the phenomenon that the nutrients and the supplemented salt water in the salt pool are not uniformly precipitated during a long culture process, and affect the accuracy of the test data. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a cross-sectional view of the present application without disc spring;

[0018] Figure 2 It is a four-part cross-sectional view of the overall structure of the present application;

[0019] Figure 3 It is a vertical cross-sectional view of the overall structure of the present application.

[0020] In the figure: 1, identification pool; 2, culture floating plate; 201, culture port; 202, culture net; 3, support base; 4, moving track; 401, positioning hole; 5, moving support plate; 501, moving knob; 6, motor; 601, rotating shaft magnetic strip; 602, rotor; 7, main rotating cylinder; 701, first auxiliary rotating cylinder; 702, second auxiliary rotating cylinder; 703, fan blade; 8, disc spring; 801, threaded groove. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the present application.

[0022] Please refer to Figures 1-2The utility model provides a kind of rice seedling root length no injury fast detection salt-tolerant identification device, including identification pool 1, the front of identification pool 1 is transparent observation window, culture float plate 2 is placed in the middle of identification pool 1, culture float plate 2 is evenly provided with culture port 201 in the middle, culture net 202 is fixedly installed at the bottom of culture port 201, culture float plate 2 floats with the liquid level height of identification pool 1, ensure that culture net 202 is always in contact with the liquid level in identification pool 1, the both sides of the bottom of identification pool 1 are fixedly connected with support base 3, the front and back of the middle of support base 3 are fixedly connected with moving track 4, sliding slot is opened in the middle of moving track 4, and moving support plate 5 is movably connected by sliding slot, the sliding slot on the front side moving track 4 of support base 3 penetrates the moving track 4 of this side, the front end of moving support plate 5 passes through the sliding slot and reaches the outside of moving track 4 by moving track 4, moving knob 501 is fixedly connected to the front of moving support plate 5, the sliding slot on the back side moving track 4 of support base 3 is located in the inside of this moving track 4 and is not penetrated, positioning hole 401 is opened in the position corresponding to the center of culture port 201 in sliding slot, protruding shaft is fixedly connected to the back of moving support plate 5, and the diameter of shaft is less than the inner diameter of positioning hole 401, so that when moving support plate 5 moves to positioning hole 401 along sliding slot, protruding shaft is embedded in positioning hole 401 by pushing moving knob 501, to ensure that the position of moving support plate 5 is fixed, the opening position of positioning hole 401 is consistent with the opening position of culture port 201, so that the fixed position of moving support plate 5 is directly below culture port 201, motor 6 is fixedly installed on the top of moving support plate 5, rotating shaft magnetic strip 601 is fixedly connected to the rotating shaft of motor 6 along horizontal direction, rotor 602 is movably placed on the bottom surface in the inside of identification pool 1, rotor 602 can rotate and move following motor 6 by the adsorption of rotating shaft magnetic strip 601, so as to form water vortex at the bottom of culture port 201 as rotating center, form a downward pulling force on vortex center line, so as to conveniently gather and stretch rice root system at the bottom of culture port 201, so that experimenters can directly observe the growth length of rice seedling root system, without taking off the seedling from culture float plate 2 to measure, avoid the tedious operation of root length measurement and damage to rice seedling root system.

[0023] Please refer to Figure 2The bottom of the culture floating plate 2 is fixedly connected with a main rotating cylinder 7, the central axis of the main rotating cylinder 7 is consistent with the central axis of the culture port 201, a water return groove is arranged at the top of the main rotating cylinder 7, the water vortex is formed by the main rotating cylinder 7, the roots of the adjacent culture port 201 are avoided to be involved in the measurement of the roots of the culture port 201, the roots are avoided to be damaged by being pulled, a first auxiliary rotating cylinder 701 is movably sleeved in the main rotating cylinder 7, the first auxiliary rotating cylinder 701 can move up and down along the main rotating cylinder 7, a limiting ring is arranged at the top of the first auxiliary rotating cylinder 701 to avoid the first auxiliary rotating cylinder 701 from being separated from the main rotating cylinder 7, a second auxiliary rotating cylinder 702 is movably sleeved in the first auxiliary rotating cylinder 701, the second auxiliary rotating cylinder 702 can move up and down along the first auxiliary rotating cylinder 701, a limiting ring is arranged at the top of the second auxiliary rotating cylinder 702 to avoid the second auxiliary rotating cylinder 702 from being separated from the first auxiliary rotating cylinder 701, the length of the single main rotating cylinder 7 is avoided to be fixed, the floating height of the culture floating plate 2 is avoided to be limited, the culture floating plate 2 is avoided to be lifted by the main rotating cylinder 7 when the water level in the identification tank 1 is low, and the culture net 202 is avoided to be separated from the water surface, the main rotating cylinder 7, the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 are all made of transparent materials.

[0024] Please refer to Figure 3 The inner walls of the main rotating cylinder 7 and the first auxiliary rotating cylinder 701 are provided with threaded grooves 801, rotating shafts are arranged at the two sides of the top of the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702, the rotating shaft at the top of the second auxiliary rotating cylinder 702 is movably connected with the threaded groove 801 of the inner wall of the first auxiliary rotating cylinder 701, and the rotating shaft at the top of the first auxiliary rotating cylinder 701 is movably connected with the threaded groove 801 of the inner wall of the main rotating cylinder 7, so that the second auxiliary rotating cylinder 702 and the first auxiliary rotating cylinder 701 can be respectively rotated to the inside of the first auxiliary rotating cylinder 701 and the main rotating cylinder 7 along the threaded groove 801, a disc spring 8 is fixedly arranged in the middle of the main rotating cylinder 7 and the first auxiliary rotating cylinder 701, and a disc spring 8 is fixedly arranged in the middle of the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702, the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 are pulled up to be shrunk into the main rotating cylinder 7 in the state that the disc spring 8 is not stressed, so that the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 do not limit the free growth of the rice seedling roots in the normal state, the bottom of the middle of the second auxiliary rotating cylinder 702 is fixedly provided with a fan blade 703, when the rice seedling roots need to be measured, the rotor 602 is driven to rotate by the moving plate 5 to form a water vortex under the main rotating cylinder 7, the water vortex drives the fan blade 703 to rotate the second auxiliary rotating cylinder 702 along the threaded groove 801, so that the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 are rotated out of the main rotating cylinder 7 downward against the elastic force of the disc spring 8, the central axis of the water vortex is stable during the measurement, and the rice seedlings on the two sides are isolated to avoid damage to the rice seedlings.

[0025] The working principle of the use method of the present application is as follows:

[0026] When in use, the rice culture solution with the required salinity for the salt tolerance identification test is injected into the identification pool 1, and the culture float plate 2 is placed to float on the culture solution, and the rice seeds to be identified are placed on the culture net 202 in the culture port 201 for culture. When the root system needs to be measured for the growth length, the moving knob 501 is slid to move the moving plate 5 to the position below the culture port 201 where the seeds to be detected grow, and the moving knob 501 is pushed inward, so that the protruding shaft on the back of the moving plate 5 is embedded into the positioning hole 401, thereby fixing the position of the motor 6, so that the center line of the water vortex generated by the rotation of the rotor 602 is consistent with the center line of the culture port 201. At this time, the power supply of the motor 6 is turned on, the rotation of the shaft magnetic strip 601 drives the rotor 602 to rotate, and the water vortex generated by the rotation drives the fan blade 703 to rotate, so that the second auxiliary rotating cylinder 702 is rotated along the thread groove 801 to overcome the elastic force of the coil spring 8 and rotate the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 out of the main rotating cylinder 7. The water flow generated in the main rotating cylinder 7 along the center line is thrown outwards by the rotor 602 from the bottom of the second auxiliary rotating cylinder 702, and at the same time, the culture solution is supplemented into the backwater tank above the main rotating cylinder 7, forming a water flow force from top to bottom in the main rotating cylinder 7, which pulls the root system in the main rotating cylinder 7 straight down, so that the growth length of the rice seedling root system can be observed directly by the experimenter, without the need to take the seedling out of the culture float plate 2 for measurement, avoiding the tedious operation of root length measurement and damage to the rice seedling root system. After the measurement and recording are completed, the power supply of the motor 6 is turned off, and the rotor 602 stops rotating, so that the fan blade 703 is not driven by the water vortex, and at this time, the first auxiliary rotating cylinder 701 and the second auxiliary rotating cylinder 702 are lifted by the elastic force of the coil spring 8 and are retracted into the main rotating cylinder 7, without limiting the free growth of the rice seedling root system. When the length of the root system under other culture ports 201 needs to be measured, the moving knob 501 is pulled back to make the protruding shaft on the back of the moving plate 5 disengage from the positioning hole 401, and then the moving knob 501 is actuated again to the position to be measured, and the above measurement method is repeated to quickly and non-destructively measure the root length.

[0027] It should be noted that, in the present document, the terms such as first and second, etc. are used merely to differentiate one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Also, the terms "comprises", "comprising", or any other variations thereof are intended to cover a non-exclusive inclusion, so that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0028] While embodiments of the application have been shown and described, it is to be understood that the embodiments described are merely exemplary of the principles and application of the present application. Numerous modifications and adaptions can be effected without departing from the spirit and scope of the present application, which is not limited to the exact construction and arrangement described. It is intended, therefore, to cover all modifications and adaptions that fall within the scope of the claims and their equivalents.

Claims

1. A rapid, non-damaging detection device for salt tolerance of rice seedling root length, comprising an identification pool (1), characterized in that: The front side of the identification pool (1) is a transparent observation window, the middle of the identification pool (1) movably places a culture floating plate (2), the middle of the culture floating plate (2) uniformly opens a culture port (201), the bottom of the culture port (201) is fixedly installed with a culture net (202), the two sides of the bottom of the identification pool (1) are fixedly connected with supporting bases (3), the front side and the back side of the middle of the supporting base (3) are fixedly connected with moving rails (4), the middle of the moving rail (4) is provided with a sliding groove, and the moving rail (4) is movably connected with a moving supporting plate (5) through the sliding groove, the sliding groove on the moving rail (4) on the front side of the supporting base (3) penetrates the moving rail (4) on the front side, one end of the front side of the moving supporting plate (5) passes through the moving rail (4) to the outside of the moving rail (4) through the sliding groove, the front side of the moving supporting plate (5) is fixedly connected with a moving knob (501), the sliding groove on the moving rail (4) on the back side of the supporting base (3) is located on the inside of the moving rail (4) and is not penetrated, the sliding groove on the moving rail (4) on the back side of the supporting base (3) is provided with a positioning hole (401) at a position corresponding to the center of the culture port (201), the top of the moving supporting plate (5) is fixedly installed with a motor (6), the rotating shaft of the motor (6) is fixedly connected with a rotating shaft magnetic strip (601) in the horizontal direction, the inside of the identification pool (1) movably places a rotor (602) on the bottom surface, the bottom of the culture floating plate (2) is fixedly connected with a main rotating cylinder (7), the top of the main rotating cylinder (7) is provided with a backwater groove at the connecting position with the bottom surface of the culture floating plate (2), the inside of the main rotating cylinder (7) movably sleeves a first auxiliary rotating cylinder (701), the inside of the first auxiliary rotating cylinder (701) movably sleeves a second auxiliary rotating cylinder (702). The middle of the main rotating cylinder (7) and the first auxiliary rotating cylinder (701) is fixedly installed with a disc spring (8), the middle of the first auxiliary rotating cylinder (701) and the second auxiliary rotating cylinder (702) is fixedly installed with a disc spring (8), the bottom of the middle of the second auxiliary rotating cylinder (702) is fixedly installed with a fan blade (703).

2. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 1, characterized in that: The culture floating plate (2) floats with the liquid level of the identification pool (1), so that the culture net (202) is always in contact with the liquid level of the identification pool (1).

3. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 1, characterized in that: The back side of the moving supporting plate (5) is fixedly connected with a protruding clamping shaft, and the diameter of the protruding clamping shaft is smaller than the inner diameter of the positioning hole (401), and the opening position of the positioning hole (401) is consistent with the opening position of the culture port (201).

4. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 1, characterized in that: The central axis of the main rotating cylinder (7) is consistent with the central axis of the culture port (201).

5. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 1, characterized in that: The first auxiliary rotating cylinder (701) can move up and down along the main rotating cylinder (7), the top of the first auxiliary rotating cylinder (701) is provided with a limiting ring to prevent the first auxiliary rotating cylinder (701) from separating from the inside of the main rotating cylinder (7), the second auxiliary rotating cylinder (702) can move up and down along the first auxiliary rotating cylinder (701), and the top of the second auxiliary rotating cylinder (702) is provided with a limiting ring to prevent the second auxiliary rotating cylinder (702) from separating from the inside of the first auxiliary rotating cylinder (701).

6. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 1, characterized in that: The inner wall of the main rotary cylinder (7) and the first auxiliary rotary cylinder (701) is provided with a threaded groove (801), the first auxiliary rotary cylinder (701) and the second auxiliary rotary cylinder (702) are provided with rotary shafts at both sides of the top, the rotary shaft at the top of the second auxiliary rotary cylinder (702) is movably connected with the threaded groove (801) of the inner wall of the first auxiliary rotary cylinder (701), and the rotary shaft at the top of the first auxiliary rotary cylinder (701) is movably connected with the threaded groove (801) of the inner wall of the main rotary cylinder (7).

7. The device for identifying salt tolerance of rice seedlings by non-destructive rapid detection of root length at seedling stage according to claim 6, characterized in that: The disc spring (8) is in a non-stressed state, and the first auxiliary rotary cylinder (701) and the second auxiliary rotary cylinder (702) are pulled up and contracted into the main rotary cylinder (7).

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