A foramsulfuron hapten, artificial antigen, antibody and preparation method and application thereof

By designing a hapten with a carboxyl group on the benzene ring of pyrifensulfuron-methyl, the problems of complex and costly detection methods for pyrifensulfuron-methyl are solved, and rapid detection with high sensitivity and specificity is achieved, making it suitable for large-scale on-site detection.

CN119330889BActive Publication Date: 2026-07-03SHENZHEN HAIJIXING AGRI PROD TESTING SCI & TECH CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN HAIJIXING AGRI PROD TESTING SCI & TECH CENT
Filing Date
2024-10-28
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing methods for detecting pyrimethanil residues are cumbersome, time-consuming, costly, and unsuitable for rapid, large-scale on-site testing. They also lack pyrimethanil haptens, artificial antigens, and antibodies, failing to meet the need for rapid and convenient testing.

Method used

A pyrimethanil hapten was designed, and a carboxyl active group with a certain length of spacer arm was introduced onto the benzene ring for coupling with a carrier protein to prepare an artificial antigen, enhance immunogenicity, and stimulate animals to produce antibodies with higher specificity and sensitivity.

Benefits of technology

By preparing haptens and artificial antigens, antibodies with high sensitivity and specificity were obtained, enabling efficient and rapid detection of pyrimethanil, reducing false positives and false negatives. The detection limit was 2.30 ng/mL, and the linear range was 4.87–63.94 ng/mL.

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Abstract

This invention discloses a pyrifensulfuron hapten, an artificial antigen, an antibody, and their preparation methods and applications. The structure of the pyrifensulfuron hapten is shown in Formula (I). This invention obtains a hapten by introducing a carboxyl active group with a certain length of spacer arm onto the benzene ring of pyrifensulfuron, which can then be coupled with a carrier protein to obtain an artificial antigen for immunization. This pyrifensulfuron hapten retains all the characteristic groups of pyrifensulfuron, with minimal alteration to the original structural features of pyrifensulfuron, laying the foundation for subsequent stimulation of animal immune responses to produce antibodies with higher specificity and sensitivity. The artificial antigen and antibody are then prepared using the above hapten. The resulting pyrifensulfuron monoclonal antibody, based on an indirect competitive ELISA using the pyrifensulfuron monoclonal antibody, shows a limit of detection of 2.30 ng / mL for pyrifensulfuron, with an IC50 value of [missing value]. 50 The effective concentration was 17.65 ng / mL, with a linear range of 4.87–63.94 ng / mL, indicating high detection sensitivity and a wide linear range.
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