Application of diphenylamine in preparation of oncomelania killing medicine

Abstract

The invention provides application of diphenylamine in preparation of oncomelania killing drugs, and belongs to the technical field of schistosome prevention and treatment. The oncomelania killing agent is prepared by taking diphenylamine as an active component through a conventional means of medicine preparation, and the oncomelania killing agent can be prepared into a suspending agent, an emulsion and a microemulsion. When the oncomelania killing agent taking the diphenylamine as the active component is used, the use amount of the active component is 1.00-10.00 g / m < 2 >, and the acting time is 7 days. Experiments prove that diphenylamine has killing activity on oncomelania and can be prepared into oncomelania killing preparations to be applied to prevention and treatment of blood sucking worms.

Description

Technical Field

[0001] The invention relates to the field of schistosomiasis control, and in particular to application of diphenylamine in the preparation of snail-killing drugs. Background Art

[0002] Schistosomiasis is a tropical parasitic disease caused by Schistosoma japonicum. There are six types of schistosomes that can parasitize humans: Schistosoma japonicum is mainly prevalent in China, the Philippines, and Indonesia, Schistosoma mansoni is mainly prevalent in Africa, the Arabian Peninsula, and South American countries, Schistosoma haematobium is mainly prevalent in Africa and the Arabian Peninsula, Schistosoma intercalatum is mainly prevalent in some African countries, and the distribution of Schistosoma mekongense and Schistosoma malayi is limited to individual countries in Southeast Asia. In the life history of Schistosoma japonicum, Oncomelania hupensis is the only intermediate host of Schistosoma japonicum and is one of the key links in the transmission and prevalence of schistosomiasis. Killing snails is an important part of controlling Schistosomiasis japonicum, so the development of new snail-killing drugs has broad application prospects and huge market value.

[0003] Diphenylamine is an organic compound with the chemical formula C 12 H 11 N is a white crystalline powder, mainly used to make lubricant antioxidants, plastic antioxidants, rubber antioxidants, gunpowder stabilizers, and is also used as an intermediate for dyes and pesticides. Summary of the invention

[0004] The purpose of the present invention is to provide a snail-killing drug with diphenylamine as the main active ingredient in view of the deficiencies of the prior art. The snail-killing drug with diphenylamine as the main active ingredient of the present invention has a killing effect on snails and can be prepared into a snail-killing agent to be applied in the field of schistosomiasis prevention and control.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A snail-killing agent comprises at least diphenylamine as an active ingredient.

[0007] Preferably, as a preferred embodiment, the snailicide may also be added with one or more conventional auxiliary ingredients in the formulation processing, including but not limited to diluents, fillers, adhesives, wetting agents, absorption promoters, surfactants, adsorption carriers, lubricants, etc. These auxiliary ingredients are various ingredients commonly used or allowed to be used in pesticide formulations, and are not particularly limited. The specific ingredients and amounts can be determined by experiments according to the formulation requirements.

[0008] Preferably, as a preferred embodiment, the formulation of the snailicide includes but is not limited to wettable powder, suspension, microemulsion, emulsion, and water dispersible granules.

[0009] Preferably, as a preferred embodiment, the amount of the snail-killing drug applied is calculated based on the area of ​​the water body so that the concentration of the active ingredient in the water body is 1.00-10.00 g / m 2 .

[0010] Another object of the present invention is to provide an application of diphenylamine for killing Oncomelania snails.

[0011] Preferably, as a preferred embodiment, the Oncomelania molluscicidal agent of the present invention may contain one or more other Oncomelania molluscicidal active ingredients in addition to diphenylamine.

[0012] Compared with the prior art, the beneficial effects of the snail-killing agent of the present invention are:

[0013] The present invention proposes for the first time the use of diphenylamine as an active ingredient of a molluscicide, and provides guidance for the application of molluscicides. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0015] 1. Test Example

[0016] The killing effect of diphenylamine and niclosamide on Oncomelania snails

[0017] The drug concentration was set from 2 g / m 2 Start by diluting the solution in 5 multiples. Weigh 500g of experimental soil and place it in a pre-prepared enamel tray. Use a watering can to spray 125ml of dechlorinated water on the surface of the soil to prepare the mud tray. Put 100 snails in each mud tray. Put the weighed medicine and a small amount of fine soil into an ep tube, add a small amount of grinding beads, grind in a low-temperature freezing grinder, and mix well. Sprinkle the mixed medicine evenly in the mud tray through a sieve. Culture the snails at each concentration for 1 day, 3 days, and 7 days, pick them out, wash them, and continue to culture them for 2 days. After two days, break the snail shells to determine their survival. The results are shown in Table 1.

[0018] Table 1 The killing effect of diphenylamine on Oncomelania snails by spreading

[0019]

[0020] Comparative Example: Using the conventional agent niclosamide as the control agent, the snail killing test was carried out according to the above method. The results are shown in Table 2:

[0021] Table 2 The killing effect of niclosamide on Oncomelania snails by broadcasting

[0022]

[0023] From the data in Tables 1 and 2, it can be seen that the killing effect of diphenylamine on Oncomelania hupensis was poor on the first day of application, but after the seventh day of application, the concentration of 2 g / m 2 The killing effect of diphenylamine on snails is up to 82%. Although the killing effect is lower than that of the conventional agent niclosamide, there is no record of the application of diphenylamine in the prior art. Moreover, it shows a good killing effect on Oncomelania hupensis, and is worthy of promotion and application.

[0024] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Any technician familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by a person of ordinary skill in the art without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A molluscicide, characterized in that: The main active ingredient of the snail-killing agent is diphenylamine.

2. A molluscicide according to claim 1, characterized in that: The molluscicide can be prepared into any dosage form used in agriculture.

3. A molluscicide according to claim 2, characterized in that: The dosage form of the snailicide includes but is not limited to wettable powder, suspension, microemulsion, emulsion, and water dispersible granules.

4. The molluscicide according to any one of claims 1 to 3, characterized in that The molluscicide may further comprise one or more other molluscicidal active ingredients.

5. Use of the Oncomelania molluscicidal agent according to any one of claims 1 to 3 for killing Oncomelania molluscs.

6. The use of diphenylamine in the preparation of drugs for killing Oncomelania snails.

7. The use according to claim 6, characterized in that: The diphenylamine is used as the active ingredient of the molluscicide in an amount of 1.00-10.00 g / m 2 , the effect lasts for 7 days.

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