Osteomyelitis wound microenvironment detection system

Abstract

The invention discloses an osteomyelitis wound microenvironment detection system, and relates to the technical field of osteomyelitis wound microenvironment detection.The osteomyelitis wound microenvironment detection system comprises a leg placing support, a detection support is arranged at the top of the leg placing support and comprises a positioning support and a movable support, and a sensor mechanism is arranged at the end, close to a buckle, of the movable support; the height and the position of the detection support are adjusted, so that the position of a wound surface suitable for a patient can be conveniently selected according to the height and the weight of the patient, and the patient can conveniently take the wound surface of the patient. The sensor array, the electrode system, the signal converter and the data processing module are integrated in the portable equipment, so that relevant indexes such as pH value, humidity and pathogenic microorganisms around a bone infection wound surface can be monitored in real time, various environmental parameters are effectively and comprehensively processed and analyzed, more comprehensive microenvironment information is provided, and the application prospect is wide. And bone infection wounds can be accurately monitored for a long time.

Description

Technical Field

[0001] The invention relates to the technical field of osteomyelitis wound microenvironment detection, and in particular to an osteomyelitis wound microenvironment detection system. Background Art

[0002] Diabetic foot is one of the serious chronic complications that causes disability and death in diabetic patients in my country. It has a high incidence rate, is difficult to treat, and is very expensive. Studies have shown that among all non-traumatic low-level amputations, diabetic patients account for 40% to 60%, and 85% of amputations occur after foot ulcers. At present, the early diagnosis of diabetic foot osteomyelitis is based on clinical manifestations, laboratory tests, and imaging evaluations. Related studies have shown that the risk of diabetic foot osteomyelitis can be assessed based on the depth and width of the patient's foot ulcer. For ulcers with a width greater than 2cm, the sensitivity is 56% and the specificity is 92%. Ulcers with a depth greater than 3mm are more likely to develop osteomyelitis than superficial ulcers (82% vs 33%).

[0003] The main methods for detecting pathogenic microorganisms in wounds include culture method, immunological detection method and molecular biology method. Among them, the traditional culture method can accurately know the number and type of pathogenic microorganisms infected in the sample, and is considered to be the "gold standard" and is often used as a control method. However, this method is time-consuming and labor-intensive, has strict requirements on the culture environment and operators, and is not suitable for the rapid diagnosis of pathogenic microorganisms. Immunological detection methods have the problem of false negatives in the "window period", which can easily lead to missed diagnoses. Polymerase chain reaction (PCR) and its derivative technologies are prone to cross-contamination, and false positive results are prone to occur. Therefore, finding a convenient and efficient method for diagnosing osteomyelitis is of great significance for the early clinical diagnosis and antibiotic selection of diabetic foot osteomyelitis. Summary of the invention

[0004] The purpose of the present invention is to provide a osteomyelitis wound microenvironment detection system to solve the above problems.

[0005] In order to solve the above technical problems, the present invention specifically provides the following technical solutions:

[0006] A system for detecting microenvironment of osteomyelitis wound surface, comprising a leg rest stand, a detection stand being arranged on the top of the leg rest stand, the detection stand comprising a positioning stand and a movable stand, a buckle being arranged between the positioning stand and the movable stand, the buckle being slidably connected to the positioning stand, the movable stand being L-shaped, one end of the movable stand being fixedly connected to the positioning stand by the buckle, and the movable stand and the positioning stand being fixedly connected to form a U-shape, a sensor mechanism being arranged at one end of the movable stand close to the buckle, the sensor mechanism being located directly above the leg rest stand, a display being arranged on one side of the leg rest stand, and the sensor mechanism being electrically connected to the display via a data conduction line.

[0007] As a preferred solution of the present invention, the sensor mechanism includes a pH sensor, a humidity sensor and a pathogenic microorganism sensor. A sensor adjustment slide rail is fixedly connected to one side of the movable bracket, and the sensor adjustment slide rail is located directly above the leg support. A plurality of sensor mounting slots are slidably connected to the sensor adjustment slide rail. The pH sensor, humidity sensor and pathogenic microorganism sensor are respectively installed at the bottom of all sensor mounting slots in a one-to-one manner, and the pH sensor, humidity sensor and pathogenic microorganism sensor are electrically connected to the display.

[0008] As a preferred solution of the present invention, the bottom of the positioning bracket and the movable bracket are both provided with adjustment sliding columns, and the two adjustment sliding columns are symmetrically arranged on both sides of the top of the leg support.

[0009] As a preferred solution of the present invention, the two adjusting sliding columns have the same structure, and the adjusting sliding columns include a movable column, a sliding rod and a locking piece for limiting the movement of the sliding rod. The movable column is vertically arranged on the top of the leg support, the sliding rod is slidably arranged inside the movable column, and the top of the sliding rod extends to the outside of the movable column. The top of the sliding rod is fixedly connected to a mounting plate, and the mounting plate is fixedly connected to the bottom end of the positioning bracket or the movable bracket. The locking piece is installed on one side of the movable column and cooperates with the sliding rod.

[0010] As a preferred solution of the present invention, movable slide rails are provided on both sides of the top of the leg support, and sliders are slidably connected to the two movable slide rails, and the two adjusting slide columns are fixedly connected to the two sliders respectively.

[0011] As a preferred solution of the present invention, the locking member includes a locking bolt, a threaded hole is formed on the side wall of the movable column, the locking bolt is threadedly connected to the threaded hole, a guide groove is formed on the outer wall of the sliding rod close to the threaded hole, and one end of the locking bolt corresponds to the position of the guide groove.

[0012] As a preferred solution of the present invention, the movable column and the sliding rod are respectively in the shape of a cylinder and a column. When the locking member releases the locking state of the sliding rod, the movable bracket can drive the sliding rod to rotate along the axis of the movable column.

[0013] As a preferred solution of the present invention, a placement box is provided on one side of the bottom of the leg rest support, a portable device is placed inside the placement box, and an electrochemical biosensor, an electrode system, a signal converter and a data processing module are installed inside the portable device.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present invention can adjust the height and position of the detection bracket through the cooperation of two adjustment sliding columns and two movable sliding rails, so that the sensor mechanism on the detection bracket can be adjusted to different positions up, down, left and right, so as to select a wound position suitable for the patient according to the patient's height and weight, thereby finding the most suitable position for detection of the patient's wound for detection.

[0016] 2. The present invention integrates the sensor array, electrode system, signal converter and data processing module into a portable device to ensure the biocompatibility and durability of the system. By selecting appropriate sensors, electrode materials, signal converters and data processing modules, an efficient and accurate bone infection wound microenvironment monitoring system is constructed to provide important reference data for clinical treatment. It is thus possible to monitor relevant indicators such as pH, humidity and pathogenic microorganisms around the bone infection wound in real time, and convert these biochemical information into measurable electrical signals through the specific recognition reaction of the electrode surface, thereby realizing quantitative or qualitative analysis of the wound microenvironment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0018] Figure 1 An overall structural schematic diagram of a system for detecting the microenvironment of a osteomyelitis wound is provided for an embodiment of the present invention;

[0019] Figure 2 A top view of a system for detecting the microenvironment of a osteomyelitis wound is provided for an embodiment of the present invention;

[0020] Figure 3 A schematic diagram of the three-dimensional structure of a detection bracket is provided for an embodiment of the present invention;

[0021] Figure 4 A side cross-sectional view of a detection bracket is provided for an embodiment of the present invention;

[0022] Figure 5 Provided for embodiments of the present invention Figure 4 A magnified view of the structure at A.

[0023] The numbers in the figure represent the following:

[0024] 1. Leg support; 2. Detection support; 3. Positioning support; 4. Movable support; 5. Buckle; 6. Sensor mechanism; 7. Display; 8. Adjusting sliding column; 9. Moving slide rail; 10. Slide block; 11. Placing box; 12. Portable device;

[0025] 61. pH sensor; 62. Humidity sensor; 63. Pathogenic microorganism sensor; 64. Sensor adjusting slide rail; 65. Sensor installation groove; 81. Movable column; 82. Sliding rod; 83. Locking part; 84. Mounting plate; 85. Locking bolt; 86. Guide groove. Detailed implementation manner

[0026] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] Embodiment 1

[0028] As Figures 1 to 5 shown, the present invention provides a detection system for the microenvironment of osteomyelitis wounds, including a leg support 1. A detection support 2 is provided on the top of the leg support 1. The detection support 2 includes a positioning support 3 and a movable support 4. A buckle 5 is provided between the positioning support 3 and the movable support 4. The buckle 5 is slidably connected to the positioning support 3. The movable support 4 is L-shaped. One end of the movable support 4 is fixedly connected to the positioning support 3 through the buckle 5, and a U-shaped is formed after the movable support 4 is fixedly connected to the positioning support 3. A sensor mechanism 6 is provided at one end of the movable support 4 close to the buckle 5. The sensor mechanism 6 is located directly above the leg support 1. A display 7 is provided on one side of the leg support 1. The sensor mechanism 6 is electrically connected to the display 7 through a data transmission wire.

[0029] Adjusting sliding columns 8 are provided at the bottoms of both the positioning support 3 and the movable support 4. The two adjusting sliding columns 8 are symmetrically arranged on both sides of the top of the leg support 1.

[0030] The two adjusting sliding columns 8 have the same structure. The adjusting sliding column 8 includes a movable column 81, a sliding rod 82, and a locking part 83 for restricting the movement of the sliding rod 82. The movable column 81 is vertically arranged on the top of the leg support 1. The sliding rod 82 is slidably arranged inside the movable column 81, and the top of the sliding rod 82 extends to the outside of the movable column 81. A mounting plate 84 is fixedly connected to the top of the sliding rod 82. The mounting plate 84 is fixedly connected to the bottom end of the positioning support 3 or the movable support 4. The locking part 83 is installed on one side of the movable column 81 and cooperates with the sliding rod 82.

[0031] Movable slide rails 9 are provided on both sides of the top of the leg support 1, and sliders 10 are slidably connected to the two movable slide rails 9, and two adjustment slide columns 8 are fixedly connected to the two sliders 10 respectively.

[0032] The locking member 83 includes a locking bolt 85. A threaded hole is formed on the side wall of the movable column 81. The locking bolt 85 is threadedly connected to the threaded hole. A guide groove 86 is formed on the outer wall of the sliding rod 82 near the threaded hole. One end of the locking bolt 85 corresponds to the position of the guide groove 86.

[0033] The movable column 81 and the sliding rod 82 are respectively in the shape of a cylinder and a column. When the locking member 83 releases the locking state of the sliding rod 82 , the movable bracket 4 can drive the sliding rod 82 to rotate along the axis of the movable column 81 .

[0034] When the present invention is in use, first open the buckle 5, then move the buckle 5 toward the positioning bracket 3, until the buckle 5 is disengaged from the movable bracket 4, rotate the movable bracket 4 to leave space above the leg support bracket 1, then put the leg support bracket 1 on the patient's legs, and fix the patient's legs on the leg support bracket 1 with straps, etc., and then rotate the movable bracket 4 back to its original position. The height of the positioning bracket 3 and the movable bracket 4 can be adjusted by two adjustment sliding columns 8. When adjusting, first release the locking state of the sliding rod 82 by the locking piece 83, rotate the locking bolt 85, and then screw the locking bolt 85 out of the threaded hole until one end of the locking bolt 85 is disengaged from the guide groove 86, then move the sliding rod 82, so as to adjust the height of the positioning bracket 3 and the movable bracket 4.

[0035] After the adjustment is completed, the locking piece 83 is reset, so that the locking bolt 85 is screwed into the guide groove 86, and the sliding rod 82 is abutted and fixed to limit the height position of the sliding rod 82. When adjusting the height of the detection bracket 2, by moving the slide rail 9 and the slider 10, it is only necessary to move the detection bracket 2 along the length direction of the leg support 1. The patient's height, weight and wound position are different. The height and position of the detection bracket 2 are adjusted through the above operation, so that the sensor mechanism 6 on the detection bracket 2 can be adjusted to different positions up, down, left and right to better adapt to the patient's wound position, so as to find the most suitable position for detection of the patient's wound, and then perform detection.

[0036] The locking member 83 may be the above-mentioned structure, or may be any structure or device in the prior art that can fix the sliding rod 82 in the movable cylinder.

[0037] The sensor mechanism 6 includes a pH sensor 61, a humidity sensor 62 and a pathogenic microorganism sensor 63. A sensor adjustment slide rail 64 is fixedly connected to one side of the movable bracket 4, and the sensor adjustment slide rail 64 is located directly above the leg support 1. A number of sensor mounting grooves 65 are slidably connected to the sensor adjustment slide rail 64. The pH sensor 61, the humidity sensor 62 and the pathogenic microorganism sensor 63 are respectively installed at the bottom of all the sensor mounting grooves 65 in a one-to-one correspondence, and the pH sensor 61, the humidity sensor 62 and the pathogenic microorganism sensor 63 are all electrically connected to the display 7.

[0038] The microenvironment of the bone infection wound is complex, and the main indicators of concern include pH value, humidity and pathogenic microorganisms. Among them, the pH sensor is used to monitor the pH around the wound, the humidity sensor 62 is used to monitor the humidity changes around the wound, and the pathogenic microorganism sensor 63 is used to detect the type and concentration of pathogenic microorganisms around the wound. The present invention is capable of in vitro real-time monitoring of the pathogenic microorganisms of the bone infection wound by setting the sensor mechanism 6, and timely warning the degree of infection progression, which plays an important guiding role in early clinical diagnosis and early intervention. In addition, non-invasive monitoring has good application prospects in future application fields due to its advantages such as high sensitivity, short detection time and high specificity. Among them, the sensor mechanism 6 may also include other sensors such as temperature sensors that can obtain data related to osteomyelitis, so as to effectively and comprehensively process and analyze multiple environmental parameters and provide more comprehensive microenvironment information.

[0039] Based on these parameters, the following are the most suitable sensor choices:

[0040] The pH sensor is preferably an ISFET pH sensor: it has the advantages of fast response and miniaturization, is suitable for real-time monitoring of pH changes in the wound microenvironment, and is more suitable for dynamic change monitoring of bone infection wounds.

[0041] The humidity sensor 62 is preferably a capacitive humidity sensor 62 , which has the characteristics of high sensitivity and fast response, and is suitable for detecting humidity changes in the wound environment, so as to facilitate monitoring of infection control and healing process.

[0042] Pathogen sensors 63 include biosensors and electrochemical immunosensors. Biosensors can accurately detect specific pathogens through specific biological recognition reactions and provide quantitative analysis, which is suitable for complex microenvironment monitoring. Electrochemical immunosensors: can detect trace pathogens through electrochemical changes caused by antibody-antigen reactions, which is suitable for portable and real-time detection.

[0043] Example 2

[0044] like Figures 1 to 5As shown, based on Example 1, a placement box 11 is provided on one side of the bottom of the leg rest 1, a portable device 12 is placed inside the placement box 11, and an electrochemical biosensor, an electrode system, a signal converter and a data processing module are installed inside the portable device 12.

[0045] The microenvironment characteristics of bone infection wounds are a complex and dynamically changing process involving multiple factors.

[0046] 1. Biological factors

[0047] 1. Bacterial load and microbial flora: Bone infection wounds are often accompanied by high bacterial loads, forming a complex microbial flora. These microorganisms not only participate in the inflammatory response of the wound, but may also produce toxins, further destroying tissue structure and function.

[0048] 2. Immune response: Infection triggers the body's immune response, including infiltration of inflammatory cells, release of cytokines, etc. However, excessive immune response may lead to increased tissue damage, forming a vicious cycle.

[0049] 2. Physical Factors

[0050] 1. Temperature: The temperature of the infected wound surface may rise due to the heat generated by the inflammatory response and bacterial metabolism. High temperature environment may accelerate bacterial reproduction and affect enzyme activity and cell membrane stability.

[0051] 2. Humidity: Infected wounds are often accompanied by increased exudate, which leads to increased local humidity. This humid environment provides favorable conditions for bacterial reproduction and may also affect the healing speed and quality of the wound.

[0052] 3. Oxygen content: Infected wounds may have local hypoxia, which is caused by vascular spasm and reduced blood flow due to inflammatory response. The hypoxic environment may inhibit the activity of repair cells such as osteoblasts, affecting the regeneration and repair of bone tissue.

[0053] 3. Chemical factors

[0054] 1. pH: After bone injury, the pH of the injured area will change to a certain extent. In an infected state, the increase in acidic substances produced by bacterial metabolism may cause the local microenvironment to become acidic. The acidic environment may inhibit the mineralization of osteoblasts and affect the repair and reconstruction of bone tissue.

[0055] 2. Metal ions: Bone tissue contains a large amount of metal ions such as calcium and phosphorus, which play an important role in the bone repair process. However, under infection, the balance of metal ions may be broken, affecting the normal repair process of bone tissue.

[0056] 3. Growth factors and cytokines: Infection may interfere with the normal expression and secretion of growth factors and cytokines, which play a key role in the bone repair process. Their reduction or imbalance may lead to obstruction of the bone repair process.

[0057] IV. Other factors

[0058] 1. Nutritional status: The nutritional status of the patient has an important impact on the healing of bone infection wounds. Malnutrition may lead to a lack of necessary nutrients and energy support for repair cells, affecting the speed and quality of wound healing.

[0059] 2. Local pressure: The infected bone wound may be compressed or pulled by the surrounding tissues. This pressure may affect the blood circulation and nutrient supply of the wound, thereby affecting the healing process of the wound.

[0060] In summary, the microenvironmental characteristics of bone infection wounds are a complex and dynamically changing process involving biological, physical, chemical and other factors. These factors interact and influence each other, and jointly determine the healing speed and quality of bone infection wounds. Therefore, when treating bone infection, it is necessary to comprehensively consider these factors and take comprehensive treatment measures to promote wound healing and recovery.

[0061] Therefore, the present invention ensures the biocompatibility and durability of the system by integrating the electrochemical biosensor, electrode system, signal converter and data processing module into a portable device 12. By selecting appropriate sensors, electrode materials, signal converters and data processing modules, an efficient and accurate bone infection wound microenvironment monitoring system is constructed to provide important reference data for clinical treatment. It is thus possible to monitor relevant indicators such as pH, humidity and pathogenic microorganisms around the bone infection wound in real time, and convert these biochemical information into measurable electrical signals through the specific recognition reaction of the electrode surface, thereby realizing quantitative or qualitative analysis of the wound microenvironment.

[0062] Preferably, in the electrode system, the electrode material and design are crucial to the performance of the sensor. For bone infection wounds, metal or conductive polymer is used as the basic electrode material, which has good conductivity and biocompatibility. In addition, the microelectrode array can provide high spatial resolution monitoring, suitable for multi-parameter detection of complex microenvironments, and can achieve high-sensitivity signal acquisition. The electrode surface is coated with corresponding sensitive materials according to the needs of different sensors.

[0063] The basic electrode converts the biochemical signals captured by the sensor into electrical signals. The electrode design needs to ensure high sensitivity and low noise to accurately transmit tiny signal changes.

[0064] Preferably, the signal converter is a key component that converts the chemical or physical signal captured by the sensor into an electrical signal. When performing analog signal processing: use an operational amplifier to amplify the weak sensor signal and remove noise and interference through a filter. Ensure that the signal is clear and measurable, suitable for primary processing of low-level signals. And use an analog-to-digital converter: convert the amplified analog signal into a digital signal for subsequent data processing. Selecting a high-resolution ADC can provide finer measurement accuracy.

[0065] Preferably, the data processing module preferably needs to process large amounts of data quickly and provide real-time analysis. The following are suitable data processing module options:

[0066] Wireless communication module

[0067] Select type: Bluetooth Low Energy (BLE) or Zigbee module. BLE module has the advantages of low power consumption and short-distance transmission, which is suitable for application in medical equipment. Zigbee is suitable for building low-power sensor networks and is suitable for monitoring systems in a larger range. It is used to transmit data to portable devices 12 or remote servers, supporting real-time monitoring and data storage.

[0068] The converted digital signals are continuously acquired and processed by a microcontroller.

[0069] For microcontrollers, choose the ARM Cortex-M series. The ARM Cortex-M series microcontrollers have powerful data processing capabilities and low power consumption, which are suitable for real-time monitoring systems. The Cortex-M4 / M7 series is particularly suitable for occasions with high signal processing requirements. It is used for sensor data acquisition and primary processing, and has good scalability and power consumption management.

[0070] For embedded processors, choose Texas Instruments MSP430 series or NXP Coretis series. These processors have low power consumption, rich peripheral interfaces and powerful data processing capabilities, which are suitable for real-time analysis in complex environments. They are used for integrated data processing and real-time analysis, especially in portable or embedded systems.

[0071] The signal is analyzed by a specific algorithm to obtain quantitative or qualitative data of pH, humidity and pathogenic microorganisms. The analysis results are stored in the memory or transmitted to an external device for further processing. This allows the sensor performance to be tested in an in vitro simulated infected wound environment and its sensitivity, specificity and response time to be evaluated.

[0072] In practical applications, these components need to be integrated through proper circuit design and system integration, and an integrated signal processing module integrated system should be selected. This integrated module combines amplifiers, filters and analog-to-digital conversion functions, which is suitable for miniaturization and integration applications, reducing system complexity and power consumption. It is more suitable for portable or wearable monitoring devices. Ensure the efficient collaboration of sensors, electrodes, signal converters and data processing modules. Considering the strict requirements of medical applications, the system also needs to be verified for biocompatibility and tested for long-term reliability. Apply this system in an actual clinical environment to monitor the microenvironment of patients' bone infection wounds and guide doctors to perform precise treatment.

[0073] Furthermore, in the bone infection wound microenvironment monitoring system, it is very important to select appropriate sensors, electrodes, signal converters and data processing modules and achieve the best match. The following is the best matching solution based on the characteristics of the bone infection wound microenvironment:

[0074] Overall system matching: SFET pH sensor + gold electrode + operational amplifier + ARM Cortex-M4: This combination can quickly and accurately monitor pH changes and is suitable for real-time monitoring of pH in bone infection wounds.

[0075] Capacitive humidity sensor 62+platinum electrode+capacitance-to-digital converter+MSP430: This combination has the advantages of high accuracy and low power consumption in humidity monitoring and is suitable for long-term monitoring of wound humidity.

[0076] Electrochemical biosensor + gold electrode + electrochemical signal processor + NXP Coretis: suitable for high-sensitivity detection of pathogenic microorganisms, capable of early infection warning and dynamic change analysis.

[0077] These matching schemes not only ensure the accuracy and reliability of the monitoring system, but also take into account the requirements of low power consumption and integration, making the overall system suitable for complex microenvironment monitoring of bone infection wounds.

[0078] In the bone infection wound microenvironment monitoring system, the selection and combination of sensors, electrodes, signal converters and data processing modules not only play their respective roles, but also form a synergistic effect through reasonable matching.

[0079] 1. Synergy between sensors and electrodes

[0080] Synergistic effect of ISFET pH sensor and gold electrode

[0081] Physical compatibility: Gold electrodes have excellent biocompatibility and conductivity, and can stably support the sensitive layer of the ISFET pH sensor, ensuring the stability of the sensor and long-term effective operation. The smoothness and chemical stability of the gold electrode surface also contribute to the uniform coverage and reaction consistency of the sensing layer.

[0082] Signal quality enhancement: The gold electrode can effectively capture the potential changes generated by the pH sensor, reduce noise and loss in signal transmission, and ensure high-quality signal transmission to the next-level signal processing device.

[0083] Example: Synergy of electrochemical biosensors and gold electrodes

[0084] Improved specificity: Gold electrodes can be surface functionalized to immobilize specific antibodies or DNA probes, significantly improving the sensor's ability to recognize specific pathogenic microorganisms.

[0085] Signal amplification: In electrochemical sensors, the high conductivity and surface chemical activity of gold electrodes can improve the efficiency of electrochemical reactions, amplify signal output, and thus improve detection sensitivity.

[0086] 2. Synergy between sensors and signal converters

[0087] Example: Synergy between electrochemical biosensors and electrochemical signal processors

[0088] Optimize signal matching: LMP91000 can finely adjust the electrical signal output by the electrochemical biosensor, optimize the current, impedance and voltage matching, and ensure the best amplification and conversion of the signal.

[0089] Noise suppression and signal enhancement: The LMP91000 not only amplifies the weak signals output by the sensor, but also removes environmental noise through built-in filters and noise suppression functions, further improving the quality and accuracy of the signal.

[0090] Example: ISFET pH Sensor and Operational Amplifier in Action

[0091] Signal amplification and linear enhancement: The voltage signal output by the ISFET sensor is weak, and the operational amplifier can effectively amplify it while maintaining a linear relationship to ensure the accuracy of subsequent digital processing.

[0092] High input impedance matching: The high input impedance of the operational amplifier can match the output characteristics of the ISFET sensor, reduce current loss, and protect the integrity of the original signal.

[0093] 3. Synergy between electrodes and signal converters

[0094] Example: Synergy of gold electrodes and high-resolution ADC

[0095] Accurate signal capture and conversion: The high-quality signal captured by the gold electrode can be finely digitized through a high-resolution ADC, preserving subtle changes in the signal, which is critical for detecting trace chemical changes or concentrations of pathogenic microorganisms.

[0096] Low-noise transmission: Low-noise signal transmission between the gold electrode and the high-resolution ADC ensures high signal fidelity and accuracy, reducing errors and data loss.

[0097] 4. Triple synergy of sensors, electrodes and data processing modules

[0098] Example: Electrochemical Biosensor + Gold Electrode + ARMCortex-M4

[0099] Real-time response and analysis: Electrochemical biosensors generate electrochemical signals through gold electrodes, and the ARMCortex-M4 can quickly collect and analyze these signals to provide real-time monitoring of pathogen concentrations. This real-time response is critical for early warning of infection.

[0100] Multi-parameter optimized processing: Cortex-M4 can not only process a single signal, but also comprehensively process signals from multiple sensors. It uses multi-tasking processing capabilities to coordinate data from different sensors to provide comprehensive wound microenvironment analysis.

[0101] 5. Multiple synergies of the overall system

[0102] Example: ISFET pH sensor + gold electrode + operational amplifier + ARM Cortex-M4 + BLE module

[0103] Comprehensive benefits: The signal captured by the ISFET pH sensor through the gold electrode is amplified by the operational amplifier and transmitted to the ARM Cortex-M4 for real-time processing, and the BLE module is responsible for wirelessly transmitting the processed data to external devices. This multiple synergy ensures high efficiency, accuracy and low latency from sensing, signal processing to data transmission.

[0104] Low power optimization: The components in the system are carefully matched to minimize power consumption while maintaining high performance, extending the life of the device. This is especially important for portable or implantable medical devices.

[0105] The above matching not only reflects the high performance of each component when running alone, but also improves the efficiency and function of the overall system through synergy after combination. These synergies are manifested in many aspects such as improved signal quality, data processing accuracy, real-time response capability and system power consumption optimization, providing reliable technical support for the bone infection wound microenvironment monitoring system. The electrochemical biosensor technology combines the specific recognition of biomolecules with highly sensitive sensing technology, which has the characteristics of rapidity, sensitivity and easy operation. By monitoring the pH, humidity, pathogenic microorganisms and other related indicators around the bone infection wound, it is captured on the electrode surface. The base electrode acts as a signal conductor to output the specific recognition reaction signal occurring on the electrode surface, and converts it into a measurable electrical signal through a converter, thereby realizing quantitative or qualitative analysis of the wound microenvironment, avoiding conventional problems such as radiation exposure, cumbersome procedures and delayed diagnosis.

[0106] Furthermore, in the bone infection wound microenvironment monitoring system, the combination of sensors, electrodes, signal converters and data processing modules can overcome various technical difficulties and maximize benefits.

[0107] 1. Capture and amplification of weak signals

[0108] In the prior art, the signals of pH changes, humidity fluctuations, and pathogenic microorganisms in the microenvironment of bone infection wounds are usually very weak and easily interfered by environmental noise, making it difficult to achieve accurate monitoring. In addition, since the signal output by the sensor is weak, if appropriate signal processing and amplification are not performed, the signal may be lost or submerged by noise.

[0109] By combining ISFET pH sensor + gold electrode + operational amplifier, the high conductivity and stability of the gold electrode can effectively capture the weak electrical signal output by the sensor, and the operational amplifier can greatly amplify the weak signal while maintaining its linear relationship and reducing signal loss. Through this combination, high-precision pH value monitoring is achieved, and even when the signal is extremely weak, the signal can be accurately acquired and amplified to provide reliable data output. This effect is difficult to achieve with a single component.

[0110] 2. Real-time monitoring in dynamic environments

[0111] In the existing technology, the microenvironment of bone infection wounds is dynamically changing, and the pH, humidity, and concentration of pathogenic microorganisms may fluctuate at any time, requiring the system to be able to perform high-frequency, low-latency real-time monitoring. Moreover, if the system cannot process and respond to these changes in a timely manner, it will be difficult to provide real-time and effective monitoring results, affecting clinical judgment and treatment effects.

[0112] By combining electrochemical biosensors + gold electrodes + high-resolution ADC + ARMCortex-M4, the high-resolution ADC can accurately convert analog signals into digital signals, and the ARMCortex-M4 microcontroller has powerful data processing capabilities, which can analyze data in real time and respond. The gold electrode provides a stable signal source in the electrochemical reaction, ensuring the continuity and accuracy of the signal. Through this combination, the system achieves high-frequency real-time data acquisition and processing, and can track subtle changes in the microenvironment of bone infection wounds. This real-time monitoring capability greatly improves the response speed of the system, surpassing the effect of traditional monitoring methods.

[0113] 3. Multi-parameter comprehensive analysis and low power design

[0114] In the existing technology, the microenvironment of bone infection wounds involves multiple parameters, which may affect each other, requiring the system to be able to perform comprehensive analysis. In addition, in portable or implantable devices, low-power design is crucial, but multi-parameter monitoring often means higher energy consumption, making it difficult to balance system performance and power consumption.

[0115] By combining the capacitive humidity sensor 62 + platinum electrode + MSP430 microcontroller + BLE communication module, the MSP430 microcontroller has low power consumption characteristics and can process data from multiple sensors. The low power consumption characteristics of the capacitive humidity sensor 62 and the platinum electrode are combined with the power management function of the MSP430 to form a low-energy, high-performance monitoring system. The BLE communication module ensures low-power data transmission. Through this combination, the system can not only comprehensively analyze multiple environmental parameters, but also maintain low-power operation, making the device suitable for portable or long-term implantation environments, significantly extending the service life of the device.

[0116] 4. Signal interference and stability in complex environments

[0117] In the prior art, bone infection wounds are usually in a complex biological environment and may be affected by body fluids, electrolytes, and temperature changes, all of which can interfere with the sensor's signal. In addition, in long-term monitoring, the stability of the sensor and signal processing components is critical, especially in a biological environment, where electrode materials and sensors may corrode or fail.

[0118] By combining gold electrodes + operational amplifiers + low-noise ADCs + embedded processors, the corrosion resistance and biocompatibility of the gold electrodes ensure the long-term stability of the system in complex environments. The combination of operational amplifiers and low-noise ADCs effectively reduces signal interference and maintains signal purity and reliability. The powerful processing power of the embedded processor can filter and calibrate the signal, further improving the stability of the system. Through this combination, the system achieves high-stability long-term operation in complex environments, can accurately monitor changes in the wound microenvironment, and avoids the failure or accuracy loss of traditional equipment in long-term monitoring.

[0119] Through the combination and matching of the above components, the bone infection wound microenvironment monitoring system not only overcomes technical difficulties such as weak signal capture, dynamic real-time monitoring, multi-parameter comprehensive analysis, and signal interference in complex environments, but also realizes high-precision real-time monitoring of dynamic microenvironments and can quickly respond to changes in the microenvironment. In a complex biological environment, it maintains long-term stability and high reliability, has strong adaptability, and can accurately monitor bone infection wounds for a long time. In addition, while maintaining system performance, it achieves low-power design and extends the service life of the equipment.

[0120] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A system for detecting the microenvironment of osteomyelitis wounds, comprising a leg support (1), characterized in that: A detection bracket (2) is arranged on the top of the leg rest bracket (1), and the detection bracket (2) includes a positioning bracket (3) and a movable bracket (4). A buckle (5) is arranged between the positioning bracket (3) and the movable bracket (4), and the buckle (5) is slidably connected to the positioning bracket (3). The movable bracket (4) is L-shaped, and one end of the movable bracket (4) is fixedly connected to the positioning bracket (3) through the buckle (5), and the movable bracket (4) and the positioning bracket (3) form a U-shape after being fixedly connected. A sensor mechanism (6) is arranged at one end of the movable bracket (4) close to the buckle (5), and the sensor mechanism (6) is located directly above the leg rest bracket (1). A display (7) is arranged on one side of the leg rest bracket (1), and the sensor mechanism (6) is electrically connected to the display (7) through a data transmission line.

2. A osteomyelitis wound microenvironment detection system according to claim 1, characterized in that: The sensor mechanism (6) comprises a pH sensor (61), a humidity sensor (62) and a pathogenic microorganism sensor (63); a sensor adjustment slide rail (64) is fixedly connected to one side of the movable bracket (4), and the sensor adjustment slide rail (64) is located directly above the leg support (1); a plurality of sensor installation slots (65) are slidably connected to the sensor adjustment slide rail (64); the pH sensor (61), the humidity sensor (62) and the pathogenic microorganism sensor (63) are respectively installed at the bottom of all the sensor installation slots (65) in a one-to-one correspondence, and the pH sensor (61), the humidity sensor (62) and the pathogenic microorganism sensor (63) are all electrically connected to the display (7).

3. A osteomyelitis wound microenvironment detection system according to claim 1, characterized in that: The bottom of the positioning bracket (3) and the movable bracket (4) are both provided with adjustment sliding columns (8), and the two adjustment sliding columns (8) are symmetrically arranged on both sides of the top of the leg support (1).

4. A osteomyelitis wound microenvironment detection system according to claim 3, characterized in that: The two adjusting sliding columns (8) have the same structure. The adjusting sliding columns (8) include a movable column (81), a sliding rod (82) and a locking member (83) for limiting the movement of the sliding rod (82). The movable column (81) is vertically arranged on the top of the leg support (1). The sliding rod (82) is slidably arranged inside the movable column (81), and the top of the sliding rod (82) extends to the outside of the movable column (81). The top of the sliding rod (82) is fixedly connected with a mounting plate (84). The mounting plate (84) is fixedly connected to the bottom end of the positioning bracket (3) or the movable bracket (4). The locking member (83) is installed on one side of the movable column (81) and cooperates with the sliding rod (82).

5. The osteomyelitis wound microenvironment detection system according to claim 3, characterized in that: Movable slide rails (9) are provided on both sides of the top of the leg support (1), and sliders (10) are slidably connected to the two movable slide rails (9), and the two adjustment slide columns (8) are fixedly connected to the two sliders (10) respectively.

6. A osteomyelitis wound microenvironment detection system according to claim 4, characterized in that: The locking member (83) comprises a locking bolt (85), a threaded hole is formed on the side wall of the movable column (81), the locking bolt (85) is threadedly connected in the threaded hole, a guide groove (86) is formed on the outer wall of the sliding rod (82) near the threaded hole, and one end of the locking bolt (85) corresponds to the position of the guide groove (86).

7. A osteomyelitis wound microenvironment detection system according to claim 6, characterized in that: The movable column (81) and the sliding rod (82) are respectively in the shape of a cylinder and a column. When the locking member (83) releases the locking state of the sliding rod (82), the movable bracket (4) can drive the sliding rod (82) to rotate along the axis of the movable column (81).

8. The osteomyelitis wound microenvironment detection system according to claim 1, characterized in that: A placement box (11) is provided on one side of the bottom of the leg rest support (1), a portable device (12) is placed inside the placement box (11), and an electrochemical biosensor, an electrode system, a signal converter and a data processing module are installed inside the portable device (12).

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