In-situ continuous collecting and sampling device for plant root exudates

Abstract

The plant root exudates in-situ continuous collecting and sampling device comprises a collecting cylinder, a root placing frame with an opening in the upper end is arranged at the upper end of the collecting cylinder, the two opposite sides of the upper end of the root placing frame are cut downwards to form a root inlet and a root outlet respectively, and the root placing frame is filled with adsorption resin particles; a supporting net is arranged at the bottom of the root system imbedding frame, and a filter screen layer is arranged at the upper end of the supporting net; the upper end of the root system placing frame is detachably connected with a water seepage tank, the lower end of the water seepage tank is downwards provided with an isolation cover, the upper end of the water seepage tank is upwards provided with a water storage barrel which is communicated with the water seepage tank and provided with an opening in the upper end, and the opening end of the water storage barrel is detachably connected with a cover body; a plurality of micropore permeation shafts capable of extending into the root system placing frame are arranged in a manner of penetrating through the bottom of the water permeation tank. The tree root exudates collecting and sampling device is suitable for collecting and sampling tree root exudates, and can perform in-situ continuous collection and sampling at the tree growth position on the premise of not damaging trees, and the sampling result is more representative.

Description

Technical Field

[0001] The invention relates to the technical field of plant secretion collection, and in particular to an in-situ continuous collection and sampling device for plant root secretions. Background Art

[0002] The interaction between plant roots and their surroundings is a key area of ​​research in ecology, agricultural science, and environmental science. Plants release a variety of organic and inorganic compounds into the soil through their roots, which are collectively referred to as root exudates. Root exudates are not only essential for the growth and development of the plants themselves, but also play an important role in regulating the structure of soil microbial communities, promoting nutrient cycling, and resisting diseases. Therefore, a deep understanding of the properties of plant root exudates and their dynamic changes is of great significance for increasing crop yields, improving soil quality, and protecting the ecological environment.

[0003] In order to explore the mechanism of action and dynamic changes of plant root secretions, scientists have developed a variety of methods to collect and analyze these precious substances. Traditional collection and sampling methods mainly include root box experiments, hydroponics, rhizosphere flushing methods, and microdialysis technology. These methods have achieved remarkable results under laboratory conditions, but they are mostly limited to the study of small plants, especially those that can be transplanted into specific containers for operation under laboratory conditions.

[0004] Large trees, such as adult mangroves in mangroves, usually grow in special environments such as intertidal zones, and their root systems have adapted to the unique conditions of high salt and low oxygen. Due to their huge size, widespread root systems and deep underground, it is difficult to find a suitable way to approach and effectively collect their secretions. At the same time, the natural environment in which large trees are located is complex and changeable, including factors such as soil type and water conditions that affect root activity, making short-term sampling results at fixed locations unrepresentative. Summary of the invention

[0005] The purpose of the present invention is to provide an in-situ continuous collection and sampling device for plant root secretions, which is suitable for collecting and sampling tree root secretions. It can perform in-situ continuous collection and sampling at the tree growth site without causing damage to the tree, and the sampling results are more representative.

[0006] The above technical objectives of the present invention are achieved through the following technical solutions: a plant root secretion in-situ continuous collection and sampling device, comprising a collection tube, the upper end of the collection tube is provided with a root placement frame with an upper end opening, the two opposite sides of the upper end of the root placement frame are cut downward to form a root inlet and a root outlet, the root placement frame is filled with adsorption resin particles, the bottom of the root placement frame is a support net connected to the upper end of the collection tube, and the upper end of the support net is provided with a filter layer;

[0007] The upper end of the root placement frame is detachably connected to a water infiltration tank, and the lower end of the water infiltration tank is downwardly provided with an isolation cover for covering the root placement frame, and a gap is left between the inner wall of the isolation cover and the outer wall of the root placement frame, and the upper end of the water infiltration tank is upwardly provided with a water storage cylinder which is connected with the water infiltration tank and has an open upper end, and the open end of the water storage cylinder is detachably connected to a cover body, and the cover body is provided with a vent hole, and a plurality of microporous infiltration shafts that can extend into the root placement frame are arranged through the bottom of the water infiltration tank.

[0008] By adopting the above technical scheme, when collecting plant root secretions, first a sampling groove is dug around the tree to leak out some softer roots, and the collecting tube and the root placement frame are placed at the bottom of the sampling groove. The roots are bent and placed in the root placement frame, and the roots are pressed with a U-shaped fixing frame so that the roots can be bent downward and enter the root placement frame. The root placement frame is then filled with adsorption resin particles, and then the infiltration tank is connected to the upper end of the root placement frame, and the microporous penetration shaft is inserted into the adsorption resin particles, so that the isolation cover covers the root placement frame, and the air generated in the isolation cover prevents the accumulated water from entering the root placement frame, which is helpful for use in wetland areas where mangroves grow.

[0009] After the installation is completed, cover the collection cylinder, root placement frame and seepage tank with soil to allow the upper end of the water storage cylinder to leak out. Set the height of the upper end of the water storage cylinder leaking out according to the water accumulation situation. Finally, add pure water to the water storage cylinder. First, add water to make the water level lower than the limit pipe. At this time, the amount of water infiltrated by some microporous infiltration shafts is only enough to meet the absorption of the roots. After the roots recover after a certain period of time, fill the water storage cylinder with water again to make all the microporous infiltration shafts seep into the root placement frame.

[0010] The water-insoluble organic compounds and non-polar compounds in the root secretions are adsorbed by the adsorption resin particles, and the water-soluble secretions are dissolved in the water after the water volume increases in the root frame, and are collected in the collection tube through the support net. After a certain period of collection, the device is dug out, the adsorption resin particles are taken out, the secretions in the adsorption resin particles are washed out with a chemical solvent, and the solution in the collection tube is taken out, thus completing the in-situ collection and sampling of the root secretions of one side of the plant.

[0011] The present invention is further configured as follows: a limit slot is arranged upwardly relative to the bottom of the root placement frame, each limit slot is provided with a downwardly inclined fixed hook, the two limit slots are detachably connected with an elastic U-shaped fixing frame, and both ends of the U-shaped fixing frame are provided with limit hooks that cooperate with the corresponding fixed hooks.

[0012] The present invention is further configured as follows: the adsorption resin particles are non-polar resins.

[0013] The present invention is further configured as follows: the filter layer is a non-woven fabric.

[0014] The present invention is further configured as follows: a limit frame matching with the root placement frame is provided at the bottom of the water infiltration tank.

[0015] The present invention is further configured as follows: the vent hole is arranged on the side wall of the cover body.

[0016] The present invention is further configured as follows: the microporous penetration shaft comprises an inner support shaft and a microporous sponge sleeve outer covering the support shaft.

[0017] The present invention is further configured as follows: a plurality of limiting tubes for covering part of the microporous permeation axis are arranged upward from the bottom of the water seepage tank, and the height of each limiting tube is higher than the height of the microporous permeation axis in the water seepage tank.

[0018] In summary, the present invention has the following beneficial effects:

[0019] First, the collection and sampling device of the present invention performs in-situ collection around trees and continuously collects during the growth of the root system. The sampling process does not cause damage to the trees and is suitable for collecting and sampling root secretions of trees. At the same time, the process can also prevent soil accumulation from entering and polluting the soil, and is suitable for in-situ collection and sampling of mangroves.

[0020] Secondly, the present invention utilizes adsorption resin particles to adsorb water-insoluble organic compounds and non-polar compounds, while the water-soluble secretions are slowly infiltrated and dissolved by pollution-free pure water and then collected in a collection tube. This enables a more comprehensive collection of root secretions, and there is no pollution in the process, so the sampling results are more representative. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 is an exploded view of the present invention;

[0023] Figure 3 Used to show the internal structure of the root placement frame;

[0024] Figure 4 Isolation cover, limit frame and microporous penetration shaft used to display the bottom of the seepage tank;

[0025] Figure 5 Used to display the microporous penetration shaft and limiting tube in the water seepage tank;

[0026] Figure 6 It is a partial cross-sectional view used to show the internal structure of the microporous permeation shaft.

[0027] In the figure: 1. collecting cylinder; 2. root placement frame; 21. root inlet; 22. root outlet; 23. supporting net; 24. filter layer; 25. limiting slot; 26. fixing hook; 3. U-shaped fixing frame; 31. limiting hook; 4. water seepage tank; 41. limiting frame; 42. isolation cover; 43. limiting pipe; 5. water storage cylinder; 51. cover; 52. vent; 6. microporous infiltration shaft; 61. supporting shaft; 62. microporous sponge sleeve. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0029] In the description of the present invention, it is necessary to understand that the directions or positional relationships indicated by terms such as “upper”, “lower”, “left”, “right”, “front”, “back”, “inside” and “outside” are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0030] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0031] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "disposed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] Example, see Figure 1-6, a plant root secretion in-situ continuous collection and sampling device, comprising a collection tube 1, a root placement frame 2 with an upper end opening is arranged at the upper end of the collection tube 1, two opposite sides of the upper end of the root placement frame 2 are cut downward to form a root inlet 21 and a root outlet 22, and the root placement frame 2 is filled with adsorption resin particles, and the adsorption resin particles are selected from non-polar resins, which are used to adsorb water-insoluble organic compounds and non-polar compounds in the secretions. The bottom of the root placement frame 2 is a support net 23 connected to the upper end of the collection tube 1, and a filter layer 24 is arranged at the upper end of the support net 23. The filter layer 24 is a non-woven fabric to prevent the adsorption resin particles from falling into the collection tube 1. Two limit slots 25 are arranged upwardly and oppositely at the bottom of the root placement frame 2, and a downwardly inclined fixed hook 26 is arranged in each limit slot 25. The two limit slots 25 are detachably connected with an elastic U-shaped fixing frame 3, and both ends of the U-shaped fixing frame 3 are provided with a limit hook 31 that cooperates with the corresponding fixed hook 26. The U-shaped fixing frame 3 is used to bend the tree roots and fix them in the root placement frame 2.

[0033] The upper end of the root placement frame 2 is detachably connected to a water seepage tank 4, and the bottom of the water seepage tank 4 is provided with a circle of limit frames 41 that match the root placement frame 2, so that the bottom of the water seepage tank 4 can be accurately connected to the root placement frame 2, and there will be no deviation or shaking after the connection. The lower end of the water seepage tank 4 is downwardly provided with a circle of isolation cover 42 for covering the root placement frame 2, and a gap is left between the inner wall of the isolation cover 42 and the outer wall of the root placement frame 2 for the entry of tree roots. At the same time, the air in the gap can prevent the soil liquid from flowing into the root placement frame 2. The upper end of the water seepage tank 4 is upwardly provided with a water storage cylinder 5 that is connected to it and has an upper end opening. The open end of the water storage cylinder 5 is detachably connected to a cover body 51, and the cover body 51 is provided with a vent hole 52, and the vent hole 52 is arranged on the side wall of the cover body 51 to maintain the air pressure balance in the water storage cylinder 5.

[0034] A plurality of microporous infiltration shafts 6 that can extend into the root placement frame 2 are arranged through the bottom of the water seepage tank 4. The microporous infiltration shaft 6 includes a support shaft 61 located inside and a microporous sponge sleeve 62 that covers the support shaft 61. The support shaft 61 plays a role in fixing the shape, and the microporous sponge sleeve 62 plays a role in slowly seeping water downward. A plurality of limiting tubes 43 for covering part of the microporous infiltration shaft 6 are arranged upward from the bottom of the water seepage tank 4. The height of each limiting tube 43 is higher than the height of the microporous infiltration shaft 6 in the water seepage tank 4, so that when the water level in the water seepage tank 4 is lower than the limiting tube 43, only part of the microporous infiltration shaft 6 can seep water downward. Only when the water level is higher than the limiting tube 43, water enters the limiting tube 43, and all the microporous infiltration shafts 6 can seep water downward.

[0035] Usage: When collecting plant root secretions, first dig a sampling groove around the tree to leak out some softer roots, put the collecting tube 1 and the root placement frame 2 into the bottom of the sampling groove, bend the roots and place them into the root placement frame 2, and press the roots with a U-shaped fixing frame 3 so that the roots can bend downward and enter the root placement frame 2, and then fill the root placement frame 2 with adsorption resin particles, and then connect the infiltration tank 4 to the upper end of the root placement frame 2, insert the microporous penetration shaft 6 into the adsorption resin particles, and make the isolation cover 42 cover the root placement frame 2. The air generated in the isolation cover 42 prevents the accumulated water from entering the root placement frame 2, which is helpful for use in wetland areas where mangroves grow.

[0036] After the installation is completed, cover the collecting cylinder 1, the root system placement frame 2 and the seepage tank 4 with soil to allow the upper end of the water storage cylinder 5 to leak out. The height of the leakage of the upper end of the water storage cylinder 5 is set according to the water accumulation situation. Finally, add pure water to the water storage cylinder 5. First, add water to make the water level lower than the limiting pipe 43. At this time, the amount of water infiltrated by some microporous infiltration shafts 6 is only enough to meet the absorption of the roots. After the roots recover after a certain period of time, fill the water storage cylinder 5 with water again, so that all the microporous infiltration shafts 6 can seep water into the root system placement frame 2.

[0037] The water-insoluble organic compounds and non-polar compounds in the root secretions are adsorbed by the adsorption resin particles, and the water-soluble secretions are dissolved in water after the water volume in the root placement frame 2 increases, and are collected in the collection tube 1 through the support net 23. After a certain period of collection, the device is dug out, the adsorption resin particles are taken out, the secretions in the adsorption resin particles are washed out with a chemical solvent, and the solution in the collection tube 1 is taken out, and the in-situ collection and sampling of the root secretions of the plants on one side is completed.

[0038] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A device for continuously collecting and sampling plant root secretions in situ, comprising a collecting tube (1), characterized in that: The upper end of the collecting cylinder (1) is provided with a root placement frame (2) with an upper end open, and two opposite sides of the upper end of the root placement frame (2) are cut downwards to form a root inlet (21) and a root outlet (22), respectively. The root placement frame (2) is filled with adsorption resin particles, and the bottom of the root placement frame (2) is a support net (23) connected to the upper end of the collecting cylinder (1), and the upper end of the support net (23) is provided with a filter layer (24); The upper end of the root placement frame (2) is detachably connected to a water seepage tank (4); the lower end of the water seepage tank (4) is downwardly provided with an isolation cover (42) for covering the root placement frame (2); a gap is left between the inner wall of the isolation cover (42) and the outer wall of the root placement frame (2); the upper end of the water seepage tank (4) is upwardly provided with a water storage cylinder (5) which is in communication with the water seepage tank and has an open upper end; the open end of the water storage cylinder (5) is detachably connected to a cover body (51); the cover body (51) is provided with a vent hole (52); and a plurality of microporous penetration shafts (6) which are capable of extending into the root placement frame (2) are provided through the bottom of the water seepage tank (4).

2. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The bottom of the root system insertion frame (2) is provided with limit slots (25) facing upwards, each limit slot (25) is provided with a downwardly inclined fixed hook (26), the two limit slots (25) are detachably connected with an elastic U-shaped fixing frame (3), and both ends of the U-shaped fixing frame (3) are provided with limit hooks (31) matching with the corresponding fixed hooks (26).

3. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The adsorption resin particles are selected from non-polar resins.

4. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The filter layer (24) is a non-woven fabric.

5. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The bottom of the water infiltration tank (4) is provided with a limiting frame (41) that matches the root placement frame (2).

6. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The vent hole (52) is arranged on the side wall of the cover body (51).

7. The in-situ continuous collection and sampling device for plant root secretions according to claim 1, characterized in that: The microporous permeable shaft (6) comprises a supporting shaft (61) located inside and a microporous sponge sleeve (62) covering the supporting shaft (61).

8. The in-situ continuous collection and sampling device for plant root secretions according to any one of claims 1 to 7, characterized in that: A plurality of limiting tubes (43) for covering a portion of the microporous permeation shaft (6) are arranged upward at the bottom of the water seepage tank (4), and the height of each limiting tube (43) is higher than the height of the microporous permeation shaft (6) in the water seepage tank (4).

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