A method for judging the due date and true or false pregnancy of a giant panda

Abstract

The present application relates to the technical field of giant panda breeding, and discloses a method for judging the true or false pregnancy and the expected delivery date of a giant panda, which comprises the following steps: collecting and detecting the progesterone value of the urine sample of a pregnant giant panda every day after mating; counting the amount of excrement of the pregnant giant panda; taking the day when the progesterone value of the excrement of a giant panda reaches the peak as day 0, taking the number of days as the horizontal coordinate, and taking the progesterone value of the urine sample and the amount of excrement of the pregnant giant panda as the vertical coordinate, to construct a pattern diagram of the change of the progesterone value and the amount of excrement of the giant panda; and judging the true or false pregnancy and the expected delivery date of the giant panda by observing the pattern diagram. The true or false pregnancy of the giant panda can be judged by observing the decrease of food and the change of the amount of excrement of the giant panda after the C point, and the expected delivery date of the true pregnant giant panda can be predicted by the time after the progesterone value peak B point. Therefore, the management personnel can strengthen the management of the giant panda in the embryo implantation period, effectively prevent the early embryo loss of the giant panda, and improve the pregnancy rate of the giant panda and prevent the early abortion of the fetus.

Description

Technical Field

[0001] This invention relates to the field of giant panda breeding technology, specifically to a method for determining the expected delivery date and true / false pregnancy in giant pandas. Background Technology

[0002] Giant pandas are rare and protected animals in China. They are seasonal monoestrous animals, generally going into estrus once a year in the spring, with the estrus lasting one to two weeks, and giving birth in the autumn. Giant panda embryonic development exhibits typical diapause; after fertilization, the egg cell remains free within the uterus and does not immediately implant into the uterine wall. Due to the varying lengths of the blastocyst's free period, the gestation period of giant pandas varies greatly, ranging from 89 to 186 days. Giant pandas also exhibit pseudopregnancy, showing remarkably similar patterns of progesterone changes and behavioral manifestations to truly pregnant pandas. Even in late pregnancy, it is difficult to determine whether a giant panda is pregnant based on changes in maternal weight and morphology. Furthermore, giant pandas show delayed implantation after mating, with almost no changes in the mother's behavior in the months leading up to delivery, except for a decrease in appetite just before giving birth.

[0003] Currently, most methods for determining pregnancy or impending due date in giant pandas rely on reproductive hormone testing. This requires collecting fresh morning urine daily from female pandas after mating until a week postpartum or the end of a false pregnancy, and recording the total daily fecal volume. The ELISA method is used to detect progesterone levels in the urine samples, followed by data analysis using Microsoft Excel. However, this method cannot directly determine the due date after mating; it requires combining progesterone data with changes in fecal volume to analyze pregnancy status and predict the due date. Therefore, existing technologies are particularly difficult for determining the due date and identifying true or false pregnancies in giant pandas, easily leading to early embryonic loss, reduced pregnancy rates, and increased risk of early miscarriage, which is detrimental to the health of pregnant pandas. To address these issues, there is an urgent need for a method developed by those skilled in the art to predict the due date, identify true or false pregnancies, improve pregnancy rates, prevent early embryonic loss and early miscarriage, and determine the nature of the pregnancy. Summary of the Invention

[0004] This invention addresses the aforementioned technical problems by providing a method for determining the due date and true / false pregnancy in giant pandas. It solves the problems of difficulty in determining the due date and true / false pregnancy in giant pandas, easy loss of early embryos, low pregnancy rate, and high risk of early miscarriage in giant pandas in existing technologies.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] The present invention provides a method for determining the expected delivery date and true / false pregnancy in giant pandas, the method comprising the following steps:

[0007] S1: After mating, the giant pandas collect morning urine once a day and test the progesterone level in the urine samples during the giant panda's pregnancy.

[0008] S2: Count the amount of feces during the pregnancy of giant pandas to monitor their appetite.

[0009] S3: The day when the giant panda gave birth and its progesterone peak was recorded as day 0. The number of days was used as the horizontal axis, and the urine progesterone level and fecal volume during the giant panda's pregnancy were used as the vertical axis to construct a graph showing the changes in progesterone level and fecal volume in giant pandas.

[0010] S4: Record the peak progesterone level as point B, the progesterone level when fecal volume begins to decrease as point A, the progesterone level when fecal volume of a pseudopregnant panda begins to increase as point C, and the progesterone level when a cub-giving panda gives birth as point D.

[0011] S5: Combine the values ​​of points A, B, C, and D to determine the true or false pregnancy status of the giant panda and its due date.

[0012] Furthermore, the method for determining the true or false pregnancy status of the giant panda in step S5 is as follows: After point A, the giant panda begins to reduce its food intake and the amount of feces begins to decrease. If after point C, the giant panda's appetite gradually recovers and the amount of feces begins to increase, then it is a false pregnancy giant panda; if after point C, the amount of feces of the giant panda remains at a low level, then it is a true pregnancy giant panda.

[0013] Furthermore, the changes in fecal volume and reduced food intake of pregnant giant pandas can be categorized into two types: one is that after reducing food intake, their appetite is very low, they are not interested in their usual favorite foods, they occasionally eat a small amount, and their fecal volume remains at a low level with minimal fluctuations; the other is that after reducing food intake, they still eat, but their appetite is unstable, their total daily food intake is still significantly lower than normal, and their fecal volume fluctuates slightly, but there will be no continuous increase in fecal volume.

[0014] Furthermore, the pseudopregnant panda will gradually regain its appetite and increase its fecal volume after point C. By observing the panda's reduced appetite and changes in fecal volume after point C, the true or false pregnancy status of the panda can be determined.

[0015] Furthermore, in the case of a true pregnant panda, progesterone levels gradually increased 17.8±0.9 days before the peak progesterone point B; in the case of a false pregnant panda, progesterone levels gradually increased 16.7±1.4 days before the peak progesterone point B.

[0016] Furthermore, the method for determining the giant panda's due date in step S5 is as follows: When the giant panda is truly pregnant, it will give birth 22.0 ± 0.71 days after the progesterone peak value B. The due date of the giant panda can be predicted by observing the progesterone peak value B.

[0017] Compared with existing technologies, the beneficial effects of this invention are as follows: This invention can determine the true or false pregnancy status of giant pandas by observing changes in their reduced food intake and fecal volume after point C, and then predict the due date of truly pregnant giant pandas by measuring the time after the progesterone peak at point B. Predicting the due date of giant pandas helps management personnel understand the gestation stage of the pandas, facilitating enhanced pregnancy management for pandas in the embryo implantation stage; thereby effectively preventing early embryo loss, increasing the pregnancy rate, and preventing early miscarriage. Attached Figure Description

[0018] Appendix Figure 1 This is a diagram illustrating the relationship between progesterone levels and fecal volume in giant pandas in this embodiment.

[0019] Appendix Figure 2 The changes in progesterone and fecal volume in the giant pandas that gave birth in this example. Figure 1 ;

[0020] Appendix Figure 3 The changes in progesterone and fecal volume in the giant pandas that gave birth in this example. Figure 2 ;

[0021] Appendix Figure 4 This is a graph showing the changes in progesterone and fecal volume in pseudopregnant giant pandas in this embodiment. Detailed Implementation

[0022] To make the objectives and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention. Example

[0023] This embodiment uses the results of 28 matings of 11 giant pandas that participated in breeding at the Qinling Giant Panda Research Center from 2019 to 2023 for statistical verification. A total of 18 cubs were successfully born, with a birth rate of 64.3%.

[0024] See attached document Figure 1-4 This embodiment of a method for determining the due date and true / false pregnancy of giant pandas includes the following steps:

[0025] S1: After mating, morning urine was collected once a day and the progesterone level in the morning urine samples of pregnant giant pandas was tested.

[0026] S2: Count the amount of feces during the pregnancy of giant pandas to monitor their appetite.

[0027] S3: The day of the peak progesterone level for the giant panda after giving birth is recorded as day 0. A graph showing the changes in progesterone levels and fecal volume during the giant panda's pregnancy is constructed, with the number of days on the x-axis and the urinary progesterone level and fecal volume on the y-axis. (Refer to the attached graph.) Figure 1I0 is the progesterone curve, I1 is the fecal volume curve for pseudopregnancy, and I2 is the fecal volume curve for true pregnancy.

[0028] S4: Record the peak progesterone level of the cub-producing giant panda as point B, the progesterone level when the fecal volume begins to decrease as point A, the progesterone level when the fecal volume of the pseudopregnant giant panda begins to increase as point C, and the progesterone level when the cub-producing giant panda gives birth as point D.

[0029] S5: Determine the true or false pregnancy status and due date of the giant panda by combining the values ​​at points A, B, C, and D respectively: The specific method for determining the true or false pregnancy status of the giant panda is as follows: After point A, the giant panda begins to reduce its food intake and the amount of feces begins to decrease. If after point C, the giant panda's appetite gradually recovers and the amount of feces begins to increase, then the giant panda is in a false pregnancy. If the giant panda's feces remain at a low level after point C, then the giant panda is in a true pregnancy. When the giant panda is in a true pregnancy, it will give birth 22.0 ± 0.71 days after the peak progesterone level B. By observing the changes in the giant panda's reduced food intake and feces after point C, the true or false pregnancy status of the giant panda can be determined. By observing the peak progesterone level B, the due date of the giant panda can be predicted.

[0030] Among them, the progesterone level of truly pregnant giant pandas gradually increased 17.8±0.9 days before the peak progesterone point B; the progesterone level of pseudopregnant giant pandas gradually increased 16.7±1.4 days before the peak progesterone point B.

[0031] The progesterone levels and fecal volume of giant pandas after mating were statistically analyzed, and the results are shown in Table 1.

[0032] Table 1. Progesterone levels and fecal volume data of giant pandas after mating.

[0033]

[0034]

[0035]

[0036]

[0037] As shown in the table above, the changes in fecal volume and reduced food intake in truly pregnant giant pandas fall into two categories: one is characterized by a very low appetite after reduced food intake, showing no interest in their usual favorite foods, occasionally eating small amounts, and consistently low fecal volume with minimal fluctuations (see appendix). Figure 2 Another type involves continued eating after reducing food intake, but with unstable appetite and a significantly lower total daily food intake than normal. Stool volume may fluctuate slightly, but there will be no sustained increase in stool volume. (See appendix) Figure 3 ; Pandas experiencing false pregnancy will gradually regain their appetite and increase their fecal volume after point C. See appendix. Figure 4 .

[0038] From the appendix Figure 1-4 It can be seen that the method in this embodiment can determine the true or false pregnancy status of giant pandas by observing changes in their reduced food intake and fecal volume after point C. Furthermore, the estimated due date of a truly pregnant giant panda can be predicted by analyzing the time following the peak progesterone level at point B. Predicting the due date helps management personnel understand the gestation stage of the giant panda, facilitating enhanced pregnancy management for pandas in the embryo implantation stage. This effectively prevents early embryo loss, improves the pregnancy rate, and prevents early miscarriage.

[0039] The above description is merely a preferred embodiment of this application and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the invention involved in this application is not limited to the technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalent features without departing from the inventive concept. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the protection scope of this invention.

Claims

1. A method for determining the due date and true / false pregnancy of giant pandas, characterized in that: The method includes the following steps: S1: After mating, the giant pandas collect morning urine once a day and test the progesterone level in the urine samples during the giant panda's pregnancy. S2: Count the amount of feces during giant panda pregnancy; S3: The day when the giant panda gave birth and its progesterone peak was recorded as day 0. The number of days was used as the horizontal axis, and the urine progesterone level and fecal volume during the giant panda's pregnancy were used as the vertical axis to construct a graph showing the changes in progesterone level and fecal volume in giant pandas. S4: Record the peak progesterone level as point B, the progesterone level when fecal volume begins to decrease as point A, and the progesterone level when fecal volume of the pseudopregnant panda begins to increase as point C. S5: Determine whether a giant panda is truly pregnant or not: After point A, the giant panda begins to reduce its food intake and the amount of feces begins to decrease. If after point C, the giant panda's appetite gradually recovers and the amount of feces begins to increase, then it is a false pregnancy. If after point C, the giant panda's feces remain at a low level, then it is a true pregnancy. S6: When a giant panda is truly pregnant, the estimated due date is 22.0 ± 0.71 days after the peak progesterone level (B).

2. The method for determining the expected delivery date and true / false pregnancy of giant pandas according to claim 1, characterized in that: The changes in fecal volume and reduced food intake of pregnant giant pandas can be categorized into two types: one is that after reducing food intake, their appetite is very low, they are not interested in their usual favorite foods, they eat only a small amount occasionally, and their fecal volume remains at a low level with minimal fluctuations; the other is that after reducing food intake, they still eat, but their appetite is unstable, their total daily food intake is still significantly lower than normal, and their fecal volume fluctuates slightly, but there is no continuous increase in fecal volume.

3. The method for determining the expected delivery date and true / false pregnancy of giant pandas according to claim 1, characterized in that: The pseudopregnant giant pandas will reduce their food intake and gradually increase their fecal volume after point C.

4. The method for determining the expected delivery date and true / false pregnancy of giant pandas according to claim 1, characterized in that: The progesterone levels of the truly pregnant giant pandas gradually increased 17.8±0.9 days before the peak progesterone level B; the progesterone levels of the false pregnant giant pandas gradually increased 16.7±1.4 days before the peak progesterone level B.

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