Low-pressure low-oxygen biological cell experiment device

Abstract

The invention discloses a low-pressure and low-oxygen biological cell experiment device which comprises an incubator, vacuumizing equipment, gas control equipment and an air pressure linkage cavity, one side of the air pressure linkage cavity is opened and is communicated with the air pressure pipeline; a movable disc is coaxially arranged in the air pressure linkage cavity, the annular side of the movable disc is in sealed connection with the inner wall of the air pressure linkage cavity through an airtight film, a push rod is connected to the axis of the movable disc, a limiting convex ring is arranged on the push rod, a reset spring located in the air pressure linkage cavity is arranged on the push rod, and one end of the reset spring abuts against the limiting convex ring; the end of the push rod penetrates and extends out of the air pressure linkage cavity along the axis of the air pressure linkage cavity, a deflectable square plate is arranged at the end of the push rod, and a connecting rod mechanism is hinged to the square plate. Negative pressure or positive pressure feedback is conducted on air pressure changes generated by oxygen consumption or supplement in the incubator through the air pressure linkage cavity, so that the movable disc drives the square plate and the connecting rod mechanism hinged to the square plate to open / close the air inlet in the bottom of the air storage tank.

Description

Technical Field

[0001] The invention relates to the technical field of biological cell experimental devices, and in particular to a low-pressure and low-oxygen biological cell experimental device. Background Art

[0002] The low-pressure and low-oxygen biological cell experimental device is a device used to simulate the growth conditions of cells in a low-oxygen or low-pressure environment. It consists of a complete low-pressure and low-oxygen cell culture incubator system through a variety of components. It can provide cells with a precise low-oxygen or low-pressure environment and is suitable for a variety of biological and medical research.

[0003] The low-pressure and low-oxygen biological cell experimental device usually uses the box as the main structure of the device, and a sealing cover is set on the top of the box to maintain the airtightness of the internal environment and prevent outside air from entering. At the same time, a constant temperature mixer located at the bottom of the cabinet provides a stable temperature and a uniform mixing environment to ensure that the cells can be evenly distributed and grow during the culture process. Cell vessels are set on the upper surface of the constant temperature mixer, and the vessels are located in the box to ensure that the cells grow in a stable low-oxygen environment. The experimental device also includes a gas control system composed of a gas controller and a gas delivery device, as well as a vacuum pumping device for maintaining a negative pressure environment. The gas control system is used to accurately control the concentration of oxygen and carbon dioxide to ensure that the cells are cultured under optimal physiological oxygen conditions. The vacuum pumping device is connected to the box through a pipeline to adjust and maintain a low-pressure environment to ensure that the pressure in the incubator is lower than the external atmospheric pressure.

[0004] However, in the existing low-pressure and low-oxygen biological cell experimental devices, the gas regulating mechanism does not provide timely feedback on the changes in the gas content in the internal experimental environment, which easily causes the problem of unstable gas content. Summary of the invention

[0005] The purpose of the present invention is to provide a low-pressure and low-oxygen biological cell experimental device to solve the problem in the prior art that the gas control device does not replenish the consumed gas into the incubator in time and the content of the replenished gas is inaccurate.

[0006] In order to solve the above technical problems, the present invention specifically provides the following technical solutions:

[0007] The present invention provides a low-pressure and low-oxygen biological cell experimental device, comprising: an incubator, the side walls of which are respectively connected with a negative pressure pipeline, an air intake pipeline, and an air pressure pipeline, one end of the air intake pipeline is connected with a gas storage tank, and an air inlet is provided at the bottom of the gas storage tank; a vacuum pumping device, which is connected with the negative pressure pipeline, and the vacuum pumping device is used to adjust and maintain the low-pressure environment in the incubator; a gas control device, which is connected with the air inlet of the gas storage tank, and the gas control device provides low-oxygen gas for the incubator through the gas storage tank as a transfer; a gas pressure linkage cavity, one side of which is open and connected with the gas pressure pipeline; an active gas is coaxially provided in the gas pressure linkage cavity The movable plate, the ring side of the movable plate is sealed and connected to the inner wall of the air pressure linkage cavity through an airtight membrane, a push rod is slidably connected to the axis of the movable plate, a limiting convex ring is provided on the push rod, a return spring located in the air pressure linkage cavity is sleeved on the push rod, one end of the return spring abuts on the limiting convex ring, and the other end of the return spring extends toward the movable plate and can abut on the movable plate; the end of the push rod extends through the axis of the air pressure linkage cavity to the outside thereof, a deflectable square plate is provided at the end of the push rod, a connecting rod mechanism is hinged on the square plate, and the deflection action of the square plate can drive the connecting rod mechanism to open / close the air inlet at the bottom of the gas storage tank.

[0008] Furthermore, the square plate is provided with a first hinged portion for hingedly connecting the push rod, the square plate is provided with a second hinged portion for fixing one corner thereof to form a deflection fulcrum, and the square plate is provided with a third hinged portion for hingedly connecting the connecting rod mechanism.

[0009] Furthermore, the connecting rod mechanism includes a connecting rod and a valve stem hinged to each other; one end of the connecting rod is hinged to the third hinge part, and the other end of the connecting rod is hinged to the valve stem. The valve stem passes through the top of the gas storage tank and is slidably connected thereto, and the valve stem moves linearly driven by the connecting rod.

[0010] Furthermore, a sealing plug is provided at the end of the valve stem, and the sealing plug opens / closes the air inlet at the bottom of the airtight gas storage tank under the drive of the valve stem.

[0011] Furthermore, the pneumatic linkage cavity includes a large-diameter cavity and a small-diameter cavity that are coaxially connected, the movable disk is arranged in the large-diameter cavity, and the push rod passes through the small-diameter cavity from the large-diameter cavity; a connecting base embedded in the small-diameter cavity is provided at the junction of the large-diameter cavity and the small-diameter cavity, the connecting base and the movable disk are coaxially fixed, the connecting base can move between the large-diameter cavity and the small-diameter cavity, and a force storage spring is sleeved on the connecting base, one end of the force storage spring abuts against the inner wall of the large-diameter cavity, and the other end abuts against the movable disk; one end of the reset spring abuts against the limiting convex ring, and the other end of the reset spring abuts against the connecting base.

[0012] Furthermore, a mounting sleeve is provided on the outside of the small-diameter cavity, and a locking rod is movably inserted in the mounting sleeve. A compression spring is provided at the bottom of the locking rod to abut against the bottom of the mounting sleeve. The top of the locking rod passes through the mounting sleeve, and the top of the locking rod has a limiting end, which can abut against the limiting convex ring on the push rod; the ring side of the movable disk protrudes to form a connecting end, and a resisting rod is fixedly connected to the connecting end, which passes through the large-diameter cavity and enters the mounting sleeve. The end of the resisting rod has a wedge-shaped end, and the side of the locking rod is provided with a wedge-shaped notch, and the wedge-shaped end cooperates with the wedge-shaped notch so that the resisting rod can push the locking rod to make a linear motion.

[0013] Furthermore, a mounting side plate is provided on one side of the square plate, a rotatable baffle rod is provided on the mounting side plate, one end of the baffle rod is connected to the mounting side plate through an elastic member, a limiting column is provided on the side wall of the square plate, and the limiting column can push the baffle rod to deflect it during the rotation of the square plate.

[0014] Furthermore, the middle portion of the blocking rod is hinged to the mounting side plate, and one end of the blocking rod is arranged on the movement path of the limiting column.

[0015] Furthermore, the elastic member includes a tension spring; during the deflection of the square plate, the force-bearing end of the tension spring is only subjected to the gravity of the blocking rod; during the resetting of the square plate, the force-bearing end of the tension spring is subjected to the gravity of the blocking rod and the tension in the same direction as the deflection direction of the blocking rod.

[0016] Furthermore, a one-way valve is provided on the air inlet pipeline to enable the gas to flow in one direction from the gas storage tank to the incubator.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The present invention can provide a low-pressure and low-oxygen environment for low-pressure and low-oxygen biological cell experiments in the incubator by using the negative pressure pipeline and the air intake pipeline on the incubator to connect the vacuum pumping equipment and the gas control equipment respectively. At the same time, the air pressure linkage cavity provides negative pressure or positive pressure feedback to the air pressure changes caused by the consumption or supplementation of oxygen in the incubator, so that the position of the movable disk is adaptively adjusted under the drive of the airtight membrane, thereby driving the push rod connected to the other side of the movable disk to translate, and then driving the square plate and the connecting rod mechanism hinged thereon to perform the opening / closing action on the air inlet at the bottom of the gas storage tank.

[0019] 2. The present invention forms the connecting rod mechanism by hingedly connecting the connecting rod and the valve stem. The connecting rod presses down the valve stem as the square plate deflects, thereby causing it to move vertically, and then drives the sealing plug to seal the air inlet, thereby automatically preventing air from entering the gas storage tank, thereby achieving the purpose of adaptive control.

[0020] 3. The present invention, through the arrangement of the baffle rod and the elastic member, can increase the resistance encountered by the push rod when the push rod retracts to the left and drives the square plate to reset, thereby compressing the sealing plug to a certain extent, making it difficult for the sealing plug to separate from the air inlet, thereby preventing the gas in the gas control device from entering the gas storage tank prematurely and causing air leakage, thereby ensuring the accuracy of the gas content in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0022] Figure 1 A three-dimensional structural diagram of a low-pressure and low-oxygen biological cell experimental device provided by the present invention;

[0023] Figure 2 for Figure 1 A partial cross-sectional view of

[0024] Figure 3 for Figure 2 Enlarged view of middle Ⅰ;

[0025] Figure 4 for Figure 2 The enlarged view of middle Ⅱ and its perspective view;

[0026] Figure 5 for Figure 4 A schematic structural diagram of another embodiment of the present invention;

[0027] Figure 6 for Figure 5 Schematic diagram in another state;

[0028] Figure 7 for Figure 5 Schematic diagram in another state;

[0029] Figure 8 for Figure 5 Schematic diagram in another state.

[0030] The numbers in the figure represent the following:

[0031] 1. Incubator; 11. Negative pressure pipeline; 12. Air inlet pipeline; 13. Air pressure pipeline; 14. Gas storage tank; 141. Air inlet; 2. Vacuum extraction equipment; 3. Gas control equipment; 4. Air pressure linkage cavity; 401. Large diameter cavity; 402. Small diameter cavity; 41. Movable disk; 411. Connecting end; 412. Abutting rod; 413. Wedge-shaped end; 42. Airtight membrane; 43. Push rod; 431. Limiting convex ring; 432. Reset spring; 44. Square plate; 441. First hinged part; 442. Second hinged part; 443. Third hinged part; 444. Limiting column; 45. Connecting rod; 46. Valve stem; 461. Sealing plug; 47. Connecting base; 471. Force storage spring; 48. Mounting sleeve; 481. Locking rod; 482. Compression spring; 483. Limiting end; 484. Wedge-shaped notch; 5. Mounting side plate; 51. Stop rod; 52. Elastic member; 521. Tension spring. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] like Figure 1 As shown, the present invention provides a low-pressure and low-oxygen biological cell experimental device, comprising:

[0034] The incubator 1 has a negative pressure pipe 11, an air intake pipe 12, and a pressure pipe 13 connected to its side walls. One end of the air intake pipe 12 is connected to a gas storage tank 14. The bottom of the gas storage tank 14 is provided with an air inlet 141.

[0035] A vacuum pumping device 2, which is connected to the negative pressure pipe 11, and is used to adjust and maintain the low pressure environment in the incubator 1;

[0036] The gas control device 3 is connected to the gas inlet 141 of the gas storage tank 14, and the gas control device 3 provides low-oxygen gas to the incubator 1 through the gas storage tank 14 as a transfer;

[0037] The pneumatic linkage cavity 4 has an opening on one side and is connected to the pneumatic pipeline 13; a movable disk 41 is coaxially arranged in the pneumatic linkage cavity 4, and the ring side of the movable disk 41 is sealed and connected to the inner wall of the pneumatic linkage cavity 4 through an airtight membrane 42; a push rod 43 is slidably connected to the axis of the movable disk 41, and a limiting convex ring 431 is provided on the push rod 43; a return spring 432 located in the pneumatic linkage cavity 4 is sleeved on the push rod 43, and one end of the return spring 432 abuts on the limiting convex ring 431, and the other end of the return spring 432 extends toward the movable disk 41 and can abut on the movable disk 41; the end of the push rod 43 extends through the axis of the pneumatic linkage cavity 4 to the outside thereof, and a deflectable square plate 44 is provided at the end of the push rod 43, and a connecting rod mechanism is hinged on the square plate 44, and the deflection action of the square plate 44 can drive the connecting rod mechanism to open / close the air inlet 141 at the bottom of the air storage tank 14.

[0038] The low-pressure and low-oxygen biological cell experiment device provided by the present invention can provide a low-pressure and low-oxygen environment for the low-pressure and low-oxygen biological cell experiment in the incubator 1 by using the negative pressure pipe 11 and the air intake pipe 12 on the incubator 1 to connect the vacuum device 2 and the gas control device 3 respectively. At the same time, the air pressure linkage cavity 4 performs negative pressure or positive pressure feedback on the air pressure change caused by the consumption or supplementation of oxygen in the incubator 1, so that the position of the movable disk 41 is adaptively adjusted under the drive of the airtight membrane 42, thereby driving the push rod 43 connected to the other side of the movable disk 41 to translate, and then driving the square plate 44 and the connecting rod mechanism hinged thereon to perform the opening / closing action on the air inlet 141 at the bottom of the gas storage tank 14.

[0039] It should be noted that the gas control device 3 in the present invention can generate a mixed gas with a low oxygen content, such as a mixed gas with a molar ratio of CO2 to O2 of 9:1, or can generate O2 or CO2 alone, which is selected according to the type and stage of cell respiration in the cell experiment. The gas generated by the gas control device 3 is first injected into the gas storage tank 14 for storage, and then the push rod 43 drives the connecting rod mechanism to open and close the air inlet 141 to control the gas flow in the gas storage tank 14. Specifically, when the cell respiration in the incubator 1 consumes O2 to generate CO2, since the consumption is usually greater than the output, a volume difference will be formed between the two, thereby reducing the oxygen content in the incubator 1 and reducing the air pressure. The air pressure difference will first be fed back to the air pressure linkage cavity 4, so that the movable disk 41 moves to the side close to the incubator 1, and the air inlet 141 of the gas storage tank 14 is in a fully open state. At this time, it is necessary to supplement oxygen into the incubator 1 through the gas control device 3. On the contrary, when gas is replenished in the incubator 1, the air pressure in the incubator 1 continues to increase, and the air pressure change will first be fed back to the air pressure linkage cavity 4, so that the movable disk 41 moves to the side away from the incubator 1, and the air inlet 141 of the gas tank 14 is gradually in a completely closed state, thereby gradually reducing until the gas delivery path from the gas control device 3 to the gas tank 14 and from the gas tank 14 to the incubator 1 is cut off.

[0040] In the above embodiment, since O2 and CO2 jointly generate pressure on the airtight membrane 42, the consumption of O2 cannot be accurately reflected. In order to solve this problem, in the preferred embodiment provided by the present invention, the incubator 1 is provided with a substance capable of absorbing the CO2 gas generated by the cell experiment, preferably a NaOH solution. After the NaOH solution absorbs the CO2 gas in the incubator 1, the change in air pressure after the oxygen is consumed in the incubator 1 will cause a negative pressure to be formed at the airtight membrane 42, thereby causing the movable disk 41 to move closer to the incubator 1.

[0041] See also Figure 2 , Figure 4 As shown, in a preferred embodiment provided by the present invention based on the above-mentioned embodiment, a first hinge portion 441 is provided on the square plate 44 for hingedly connecting the push rod 43, a second hinge portion 442 is provided on the square plate 44 for fixing one corner of itself to form a deflection fulcrum, and a third hinge portion 443 is provided on the square plate 44 for hingedly connecting the connecting rod mechanism.

[0042] In this embodiment, the third hinge part 443 is preferably located directly above the first hinge part 441, and the second hinge part 442 is preferably located obliquely above the first hinge part 441, so that when the push rod 43 horizontally pushes the square plate 44 to deflect, the third hinge part 443 can rotate around the second hinge part 442 to link the connecting rod mechanism.

[0043] Please continue reading Figure 4As shown, in a preferred embodiment provided by the present invention based on the above-mentioned embodiment, the connecting rod mechanism includes a connecting rod 45 and a valve stem 46 which are hinged to each other; one end of the connecting rod 45 is hinged to the third hinge part 443, and the other end of the connecting rod 45 is hinged to the valve stem 46, the valve stem 46 passes through the top of the gas storage tank 14 and is slidably connected thereto, and the valve stem 46 performs linear motion driven by the connecting rod 45.

[0044] More specifically, a sealing plug 461 is provided at the end of the valve stem 46 , and the sealing plug 461 opens / closes the air inlet 141 at the bottom of the airtight air storage tank 14 under the drive of the valve stem 46 .

[0045] In this embodiment, the connecting rod 45 and the valve stem 46 are hinged to form the connecting rod mechanism, and the valve stem 46 needs to be guided by a slide to prevent the valve stem 46 from being difficult to form a vertical linear motion when directly inserted into the upper end of the gas storage tank 14, thereby affecting the air tightness of the gas storage tank 14. The connecting rod 45 presses down the valve stem 46 during the deflection of the square plate 44, so that it forms a vertical motion, and then drives the sealing plug 461 to block the air inlet 141, so as to automatically prevent the air from entering the gas storage tank 14, thereby achieving the purpose of adaptive control.

[0046] In the above embodiment, the movement state of the valve stem 46 following the movable disk 41 mainly depends on the translational movement of the movable disk 41, so that the valve stem 46 drives the sealing plug 461 to seal the air inlet 141 of the gas tank 14 only under a critical state of a certain air pressure value, and it is difficult to form a stable locking state for the air inlet 141, and thus it is impossible to accurately control the air intake of the gas tank 14 into the incubator 1.

[0047] To solve the above problems, please continue to refer to Figure 3 As shown, in a preferred embodiment provided by the present invention based on the above embodiments, the pneumatic linkage cavity 4 includes a large diameter cavity 401 and a small diameter cavity 402 that are coaxially connected, the movable disk 41 is arranged in the large diameter cavity 401, and the push rod 43 passes through the small diameter cavity 402 from the large diameter cavity 401; a connecting base 47 embedded in the small diameter cavity 402 is provided at the junction of the large diameter cavity 401 and the small diameter cavity 402, the connecting base 47 and the movable disk 41 are coaxially fixed, and the connecting base 47 can move between the large diameter cavity 401 and the small diameter cavity 402, and a force storage spring 471 is sleeved on the connecting base 47, one end of the force storage spring 471 abuts against the inner wall of the large diameter cavity 401, and the other end abuts against the movable disk 41; one end of the reset spring 432 abuts against the limiting convex ring 431, and the other end of the reset spring 432 abuts against the connecting base 47.

[0048] Please continue reading Figure 3As shown, in a preferred embodiment provided by the present invention based on the above-mentioned embodiment, a mounting sleeve 48 is provided on the outside of the small-diameter cavity 402, and a locking rod 481 is movably inserted in the mounting sleeve 48. A compression spring 482 is provided at the bottom of the locking rod 481 to abut against the bottom of the mounting sleeve 48. The top of the locking rod 481 passes through the mounting sleeve 48, and the top of the locking rod 481 has a limiting end 483, which can abut against the limiting convex ring 431 on the push rod 43; the ring side of the movable disk 41 protrudes to form a connecting end 411, and a resisting rod 412 is fixedly connected to the connecting end 411, and the resisting rod 412 passes through the large-diameter cavity 401 and enters the mounting sleeve 48. The end of the resisting rod 412 has a wedge-shaped end 413, and the side of the locking rod 481 is provided with a wedge-shaped notch 484, and the wedge-shaped end 413 cooperates with the wedge-shaped notch 484 so that the resisting rod 412 can push the locking rod 481 to make a linear motion.

[0049] In this embodiment, when the push rod 43 is pushed to the right (away from the incubator 1) by the air pressure in the incubator 1, the movable plate 41 will simultaneously press down the locking rod 481 through the abutting rod 412, thereby continuously compressing the compression spring 482. After reaching a certain compression amount, the locking rod 481 is completely pressed down to form an avoidance, so that the limiting convex ring 431 on the push rod 43 passes over the wedge-shaped end on the locking rod 481, so that when the push rod 43 retracts to the left (close to the incubator 1), it still needs to overcome the elastic force of the compression spring 482, thereby locking the position of the push rod 43. Then, when the air pressure in the air pressure linkage cavity 4 increases to a certain extent, the push rod 43 will be pushed to the right, driving the deflection of the square plate 44; conversely, when the air pressure in the air pressure linkage cavity 4 decreases to a certain extent, the push rod 43 will retract to the left, driving the square plate 44 to return to its original position.

[0050] In the above embodiment, when the push rod 43 retracts to the left and drives the square plate 44 to reset, the sealing plug 461 is more likely to separate from the air inlet 141 of the gas tank 14, thereby allowing the gas in the gas control device 3 to enter the gas tank 14 in advance, causing gas leakage, thereby affecting the accuracy of the gas content in the incubator 1.

[0051] In order to solve the above problems, Figure 5-Figure 8 As shown, in a preferred embodiment provided by the present invention based on the above-mentioned embodiment, a mounting side plate 5 is provided on one side of the square plate 44, a rotatable blocking rod 51 is provided on the mounting side plate 5, one end of the blocking rod 51 is connected to the mounting side plate 5 through an elastic member 52, and a limiting column 444 is provided on the side wall of the square plate 44, and the limiting column 444 can push the blocking rod 51 to deflect it during the rotation of the square plate 44.

[0052] More specifically, the middle portion of the blocking rod 51 is hinged to the mounting side plate 5 , and one end of the blocking rod 51 is disposed on the movement path of the limiting column 444 .

[0053] In the present embodiment, by arranging the blocking rod 51 and the elastic member 52, when the push rod 43 retracts to the left and drives the square plate 44 to reset, the resistance encountered by the retraction of the push rod 43 can be increased, thereby being able to compress the sealing plug 461 to a certain extent, making it difficult for the sealing plug 461 to separate from the air inlet 141, thereby preventing the gas in the gas control device 3 from entering the gas storage tank 14 in advance and causing air leakage, thereby ensuring the accuracy of the gas content in the incubator 1.

[0054] like Figure 5-Figure 8 As shown, in the preferred embodiment provided by the present invention based on the above embodiment, the elastic member 52 includes a tension spring 521; during the deflection of the square plate 44, the force-bearing end of the tension spring 521 is only subjected to the gravity of the blocking rod 51; during the resetting of the square plate 44, the force-bearing end of the tension spring 521 is subjected to the gravity of the blocking rod 51 and the tension in the same direction as the deflection direction of the blocking rod 51. In this embodiment, by setting the tension spring 521, it can be achieved that when the square plate 44 deflects, the tension spring 521 does not act on the blocking rod 51, and the limit column 44 pushes against one end of the blocking rod 51 to form a avoidance; when the square plate 44 is reset, the tension spring acts on the blocking rod 51, generating a pulling force on one end of the blocking rod 51, thereby increasing the resistance of the push rod 43 to retract when the square plate 44 is reset, and pressing the sealing plug 461, so that the sealing plug 461 is not easy to separate from the air inlet 141, and further ensuring the accuracy of the gas content in the incubator 1.

[0055] In order to prevent the products generated by the experiment in the incubator 1 from entering the gas storage tank 14 through the air inlet pipe 12 due to gas exchange, in a preferred embodiment provided by the present invention based on the above embodiment, a one-way valve is provided on the air inlet pipe 12 to allow the gas to flow one-way from the gas storage tank 14 to the incubator 1.

[0056] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A low-pressure and low-oxygen biological cell experimental device, comprising: An incubator (1) having side walls respectively connected to a negative pressure pipe (11), an air intake pipe (12), and an air pressure pipe (13); one end of the air intake pipe (12) is connected to an air storage tank (14); and an air intake port (141) is provided at the bottom of the air storage tank (14); A vacuum pumping device (2), which is in communication with the negative pressure pipeline (11), and the vacuum pumping device (2) is used to adjust and maintain the low pressure environment in the incubator (1); A gas control device (3) is connected to the gas inlet (141) of the gas storage tank (14), and the gas control device (3) provides low-oxygen gas to the incubator (1) through the gas storage tank (14) as a transfer; A pneumatic linkage cavity (4) having an opening on one side and communicating with the pneumatic pipeline (13); The invention is characterized in that: a movable disk (41) is coaxially arranged in the air pressure linkage cavity (4); the ring side of the movable disk (41) is sealed and connected to the inner wall of the air pressure linkage cavity (4) through an airtight film (42); a push rod (43) is slidably connected to the axis of the movable disk (41); a limiting convex ring (431) is arranged on the push rod (43); a return spring (432) located in the air pressure linkage cavity (4) is sleeved on the push rod (43); one end of the return spring (432) abuts against the limiting convex ring (431); the other end of the return spring (432) extends toward the movable disk (41) and can abut against the movable disk (41); The end of the push rod (43) extends through the axis of the air pressure linkage cavity (4) to the outside thereof, and a deflectable square plate (44) is provided at the end of the push rod (43). A connecting rod mechanism is hinged on the square plate (44), and the deflection action of the square plate (44) can drive the connecting rod mechanism to open / close the air inlet (141) at the bottom of the air storage tank (14).

2. A low-pressure and low-oxygen biological cell experimental device according to claim 1, characterized in that: The square plate (44) is provided with a first hinge portion (441) for hingedly connecting the push rod (43), the square plate (44) is provided with a second hinge portion (442) for fixing one corner of itself to form a deflection fulcrum, and the square plate (44) is provided with a third hinge portion (443) for hingedly connecting the connecting rod mechanism.

3. A low-pressure and low-oxygen biological cell experimental device according to claim 2, characterized in that: The connecting rod mechanism comprises a connecting rod (45) and a valve stem (46) which are hinged to each other; One end of the connecting rod (45) is hinged to the third hinge part (443), and the other end of the connecting rod (45) is hinged to the valve stem (46). The valve stem (46) passes through the top of the gas storage tank (14) and is slidably connected thereto. The valve stem (46) moves linearly under the drive of the connecting rod (45).

4. A low-pressure and low-oxygen biological cell experimental device according to claim 3, characterized in that: A sealing plug (461) is provided at the end of the valve stem (46), and the sealing plug (461) opens / closes the air inlet (141) at the bottom of the air storage tank (14) under the drive of the valve stem (46).

5. A low-pressure and low-oxygen biological cell experimental device according to claim 4, characterized in that: The pneumatic linkage cavity (4) comprises a large-diameter cavity (401) and a small-diameter cavity (402) which are coaxially connected, the movable disk (41) is arranged in the large-diameter cavity (401), and the push rod (43) passes through the small-diameter cavity (402) from the large-diameter cavity (401); A connecting base (47) embedded in the small diameter cavity (402) is provided at the junction of the large diameter cavity (401) and the small diameter cavity (402); the connecting base (47) and the movable disk (41) are coaxially fixedly connected; the connecting base (47) movably shuttles between the large diameter cavity (401) and the small diameter cavity (402); a force storage spring (471) is sleeved on the connecting base (47); one end of the force storage spring (471) abuts against the inner wall of the large diameter cavity (401), and the other end abuts against the movable disk (41); One end of the return spring (432) abuts against the limiting convex ring (431), and the other end of the return spring (432) abuts against the connecting base (47).

6. A low-pressure and low-oxygen biological cell experimental device according to claim 5, characterized in that: The outside of the small-diameter cavity (402) is provided with a mounting sleeve (48), and a locking rod (481) is movably inserted in the mounting sleeve (48). The bottom of the locking rod (481) is provided with a compression spring (482) that abuts against the bottom of the mounting sleeve (48), and the top of the locking rod (481) passes through the mounting sleeve (48). The top of the locking rod (481) has a limiting end (483), and the limiting end (483) can abut against the limiting convex ring (431) on the push rod (43); The annular side of the movable disk (41) protrudes to form a connecting end (411), and a resisting rod (412) is fixedly connected to the connecting end (411). The resisting rod (412) passes through the large diameter cavity (401) and enters into the mounting sleeve (48). The end of the resisting rod (412) has a wedge-shaped end (413), and the side of the locking rod (481) is provided with a wedge-shaped groove (484). The wedge-shaped end (413) cooperates with the wedge-shaped groove (484) so ​​that the resisting rod (412) can push the locking rod (481) to make a linear motion.

7. A low-pressure and low-oxygen biological cell experimental device according to claim 6, characterized in that: A mounting side plate (5) is provided on one side of the square plate (44), a rotatable blocking rod (51) is provided on the mounting side plate (5), one end of the blocking rod (51) is connected to the mounting side plate (5) via an elastic member (52), a limiting column (444) is provided on the side wall of the square plate (44), and the limiting column (444) can push against the blocking rod (51) to deflect it during the process of rotating with the square plate (44).

8. The low-pressure and low-oxygen biological cell experimental device according to claim 7, characterized in that: The middle portion of the blocking rod (51) is hinged to the mounting side plate (5), and one end of the blocking rod (51) is arranged on the movement path of the limiting column (444).

9. The low-pressure and low-oxygen biological cell experimental device according to claim 8, characterized in that: The elastic member (52) comprises a tension spring (521); During the deflection of the square plate (44), the force-bearing end of the tension spring (521) is only subjected to the gravity of the blocking rod (51); During the resetting process of the square plate (44), the force-bearing end of the tension spring (521) is subjected to the gravity of the blocking rod (51) and a tension in the same direction as the deflection direction of the blocking rod (51).

10. The low-pressure and low-oxygen biological cell experimental device according to claim 9, characterized in that: The air inlet pipeline (12) is provided with a one-way valve to enable the gas to flow from the gas storage tank (14) to the incubator (1) in one direction.

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