Method for constructing small salivary gland organs of sjogren syndrome patient

By constructing small salivary gland organoids in patients with Sjögren's syndrome, using three-dimensional scaffolding and single-cell sequencing technology, the problems of genomic instability and phenotypic changes in traditional two-dimensional cell culture are solved, and effective research on the pathological mechanism of salivary glands in patients with Sjögren's syndrome are realized and the possibility of personalized treatment is achieved.

CN119639655APending Publication Date: 2025-03-18PEKING UNIVERSITY THIRD HOSPITAL (THE THIRD CLINICAL MEDICAL SCHOOL OF PEKING UNIVERSITY)
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Patent Information

Application Number
CN202411916613.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-24
Publication Date
2025-03-18

AI Technical Summary

Technical Problem

It is difficult to effectively study and understand the pathological mechanism of salivary glands in patients with Sjögren's syndrome. Traditional two-dimensional cell cultures have problems with genomic instability and phenotypic changes.

Method used

By constructing an organoid-based disease model, specific steps include isolating adult stem cells from the salivary gland tissue of Sjogren's patients, using laminin-GelMA three-dimensional scaffolds and specific growth factors for cell differentiation and organoid formation, and combining single-cell sequencing technology to analyze the cell diversity of organoids.

Benefits of technology

The established small salivary gland organoids can faithfully simulate the pathological state of patients' salivary glands, reveal the mechanisms of the immune system destroying salivary gland cells, and the relationship with the clinical symptoms of Sjögren's syndrome, providing new avenues for personalized medicine, drug screening and gene therapy.

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Abstract

The invention provides a method for constructing a small salivary gland organ of a sjogren syndrome patient. Comprising the following steps: step 1, obtaining a sample from small salivary gland tissues of a sjogren syndrome patient; separating out main adult stem cells, filtering, and centrifuging to remove impurities, so as to obtain a single cell suspension; step 2, constructing a three-dimensional bracket and configuring a culture medium; step 3, planting the separated stem cells into a laminin-GelMA three-dimensional scaffold, and adding cell factors into a culture solution; and continuously optimizing conditions of the culture medium to form small organs with salivary gland structural characteristics. The method for constructing the small salivary gland organ of the sjogren syndrome patient not only has important significance in basic research, but also provides precious tools and platforms for future personalized treatment, drug development, immunological research and salivary gland regeneration. The development of the field is expected to bring revolutionary breakthrough in the aspect of treating sjogren syndrome.
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