SSR primer and method for identifying purity of Weihir63 spinach seeds

By using SSR primers and PCR amplification technology, the genomic DNA of Viagra 63 spinach seeds was identified, which solved the problem that seed purity identification is affected by external factors in the prior art, and achieved rapid, accurate and low-cost seed purity detection.

CN119639950APending Publication Date: 2025-03-18何春霖
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Patent Information

Application Number
CN202510033736.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-09
Publication Date
2025-03-18

AI Technical Summary

Technical Problem

In the prior art, spinach seed purity identification depends on morphological methods and is easily affected by factors such as the external environment, planting season, planting technology and experience, resulting in large workload, long cycles and inaccurate enough.

Method used

The genomic DNA of Viagra 63 spinach seeds was PCR amplified by SSR primers (upstream primer SP025-F and downstream primer SP025-R). Combined with 8% polyacrylamide gel electrophoresis and silver staining and color development technology, the seed purity was quickly and accurately identified.

Benefits of technology

The seed purity of Viagra 63 spinach seeds is quickly and accurately identified within one or two hours, avoiding the influence of external factors, reducing workload, shortening cycles, and simple and low-cost.

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Abstract

According to the SSR primer and method for identifying the purity of the Weihir63 spinach seeds, provided by the invention, the primer which can simultaneously show specific bands of a male parent and a female parent in the Weihir63 spinach seeds and has relatively strong specificity is screened and used; therefore, the problems of great influence of factors such as external environment, planting season, planting technology and experience, large workload, long period and the like caused by morphological identification of the seed purity are solved, so that the seed purity of the Weihir63 spinach seed can be rapidly and accurately identified within one or two hours; the method has the advantages of rapidness, stability, simplicity in operation, low cost, no environmental influence and the like, can be used for efficiently detecting the purity of the Weihir63 spinach seeds, and has a wide application prospect.
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