Cordyceps sinensis and method for artificially culturing new strain of cordyceps sinensis
By adding N-acetylglucosamine to the liquid culture medium of Cordyceps sinensis and using the method of infecting larvae of the small golden bat moth on the body surface, the problem of inconsistent artificial cultivation of Cordyceps sinensis in the prior art was solved, efficient artificial cultivation was achieved, and products consistent with wild Cordyceps sinensis were obtained.
Patent Information
- Application Number
- CN202411927914.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-25
- Publication Date
- 2025-03-21
AI Technical Summary
The prior art is difficult to efficiently cultivate Cordyceps sinensis in low altitude areas, especially in the body surface infection of host insect larvae, which cannot achieve high bacterial carrier rate and high zombie rate, resulting in inconsistent morphology of artificially cultivated Cordyceps sinensis.
Cordyceps sinensis bacteria were cultured using liquid culture medium containing N-acetylglucosamine. The larvae of the small golden bat moth was infected on the body surface to improve the bacterial carrying rate and zombie rate, and the fruiting morphology consistent with wild Yushu or Nagqu Cordyceps sinensis was obtained.
A 100% bacterial carriage rate and a zombie rate of more than 50% were achieved, and artificial cultivation products consistent with the morphology of wild Cordyceps sinensis were obtained.
Abstract
Description
Technical Field
[0001] The invention belongs to the field of artificial cultivation of cordyceps sinensis, and specifically relates to screened Yushu and Naqu strains of cordyceps sinensis, and a method for obtaining new cordyceps sinensis strains by using the strains to infect host insect golden bat moth larvae on their body surfaces. Background Art
[0002] Cordyceps sinensis is a rare traditional food and medicine resource in my country. It is produced in snow-capped high-altitude cold areas above 3,000 meters above sea level in Tibet, Qinghai, Yunnan, Sichuan, Gansu, etc. Cordyceps sinensis has many functions such as "tonifying the lungs, strengthening the kidneys, benefiting the essence and qi, and regulating all kinds of deficiencies and damages". These functions are derived from the antibacterial, antiviral, anti-inflammatory, anti-tumor and immune regulation functions of Cordyceps sinensis. In the traditional tonic market in my country, Cordyceps sinensis has always been respected and trusted by the people. It is widely used in medicine, food and modern biotechnology, and is popular in international markets such as Japan, South Korea, Southeast Asian countries and the United States.
[0003] The Cordyceps sinensis fungus infects the host insect - the bat moth larvae, rigidifies the larvae, and forms a complex structure with insects (rigid insects) and grass (fungal fruiting bodies) under suitable conditions, which is Cordyceps sinensis. Cordyceps sinensis belongs to the Ascomycota, Sordariomycetes, Hypocreales, Ophiocordycipitaceae, and Ophiocordyceps.
[0004] The depletion of wild Cordyceps resources, strong market demand and national policy protection have led to a surge in its market price. Wild Cordyceps has been listed as a national Class II protected species. In order to protect the ecological environment of the Qinghai-Tibet Plateau and the wild Cordyceps resources, and to allow this resource to better serve the universal "Healthy China", the only option is artificial cultivation under suitable conditions.
[0005] The artificial cultivation method of Cordyceps sinensis in low-altitude areas has been established. This method relies on the following three technological breakthroughs: first, the efficient breeding of Cordyceps sinensis host bat moth insects; second, the cultivation of Cordyceps sinensis fungi that efficiently infect the host; third, the appropriate Cordyceps sinensis fungi infect the bat moth larvae and form Cordyceps sinensis under suitable conditions.
[0006] Generally speaking, entomogenous fungi can enter the insect body through the insect body wall, natural orifices or digestive tract. In the process of artificial cultivation of Cordyceps sinensis, the current methods of artificially infecting host larvae include: collecting Cordyceps sinensis ascospores to infect bat moth larvae; applying the cultured Cordyceps sinensis spore liquid to the host body surface, feeding the spores mixed with feed to enter the host body from the esophagus; injecting blastospores into the bat moth larvae. Currently, the widely used method is to use the collected Cordyceps sinensis ascospores to infect bat moth larvae. The strains used are Cordyceps sinensis ascospores from low-altitude production areas in Gansu (mainly because the price of Gansu Cordyceps sinensis strains is low). N-acetylglucosamine (fN-acetylglucosamine) and insect ecdysone (20-hydroxyecdysone) promote the transformation of Cordyceps sinensis blastospores into hyphae in vitro, and mannitol promotes the transformation of Cordyceps sinensis blastospores into hyphae in vivo. However, the liquid cultured Cordyceps sinensis culture liquid under the existing technology cannot effectively infect the bat moth larvae on the body surface, let alone achieve a high bacterial carrying rate (100% bacterial carrying rate) and a high dead insect rate (greater than 50%), so as to obtain artificially cultivated Cordyceps sinensis with the same fruiting body morphology as wild Yushu or Nagqu Cordyceps sinensis. Summary of the invention
[0007] The technical problem solved by the present invention is to overcome the defects of the prior art and provide a method for artificially cultivating a new strain of Cordyceps sinensis. The new strain is obtained by culturing Cordyceps sinensis strains, especially strains from Yushu, Qinghai or Naqu, Tibet, and then infecting the larvae of the golden bat moth on the body surface. The larvae of the golden bat moth have a high bacterial carrying rate (100% bacterial carrying rate) and a high dead insect rate (higher than 50%). The obtained artificially cultivated Cordyceps sinensis has the same fruiting body morphology as the wild Yushu or Naqu Cordyceps sinensis.
[0008] The first object of the present invention is to provide an application of N-acetylglucosamine in promoting the infection of Cordyceps sinensis by bat moth larvae, inducing Cordyceps sinensis to grow fruiting bodies in bat moth larvae, and improving the dead insect rate of bat moth larvae.
[0009] Preferably, the N-acetylglucosamine is placed in a culture medium of Cordyceps sinensis to cultivate Cordyceps sinensis, and then the bacterial solution is used to infect the bat moth larvae.
[0010] Preferably, the concentration of the N-acetylglucosamine is 0.01 to 1% by mass, and more preferably 0.01 to 0.5% by mass.
[0011] Preferably, the Cordyceps sinensis is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206, the Ophiocordyceps sinensis QHYS2022-3 has a deposit number of GDMCC No.65577; the Ophiocordyceps sinensis XZNQ2206 has a deposit number of GDMCC No.65578.
[0012] Preferably, the bat moth larvae are Helidae larvae, especially 1-5 instar larvae.
[0013] The second object of the present invention is to provide a method for artificially cultivating a new strain of Cordyceps sinensis, comprising the following steps:
[0014] 1) adding 0.01-1% N-acetylglucosamine to a liquid culture medium of Cordyceps sinensis, and then inoculating Cordyceps sinensis for cultivation to obtain a mixture of blastospores and hyphae;
[0015] 2) Infect bat moth larvae with the mixed liquid surface, and then culture them to obtain Cordyceps sinensis.
[0016] Preferably, the Cordyceps sinensis is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206, the Ophiocordyceps sinensis QHYS2022-3 has a deposit number of GDMCC No.65577; the Ophiocordyceps sinensis XZNQ2206 has a deposit number of GDMCC No.65578.
[0017] Preferably, the bat moth larvae are Helidae larvae, especially 1-5 instar larvae.
[0018] Preferably, the mixed liquid is mixed with sterile coconut husk and then the bat moth larvae are infected on the body surface.
[0019] The invention also provides cordyceps sinensis QHYS2022-3, with a preservation number of GDMCC No.65577.
[0020] The invention also provides Ophiocordyceps sinensis XZNQ2206, with the deposit number being GDMCC No.65578.
[0021] Compared with the existing technology, the liquid surface of Cordyceps sinensis cultured in liquid culture medium supplemented with N-acetylglucosamine to infect bat moth larvae can achieve a high bacterial carrying rate (100% bacterial carrying rate) and a high dead insect rate (higher than 50%) at the same time, and obtain artificially cultivated Cordyceps sinensis with the same fruiting body morphology as wild Yushu or Nagqu Cordyceps sinensis.
[0022] Ophiocordyceps sinensis QHYS2022-3 was deposited in Guangdong Provincial Microbiological Collection Center (GDMCC) on December 3, 2024, address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, and the deposit number is: GDMCC No.65577.
[0023] Ophiocordyceps sinensis XZNQ2206 was deposited in Guangdong Provincial Microbiological Collection Center (GDMCC) on December 3, 2024, address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, and the deposit number is: GDMCCNo.65578. DETAILED DESCRIPTION
[0024] The Yushu strain of Cordyceps sinensis of the present invention - Ophiocordyceps sinensis QHYS2022-3 was isolated from Yushu, Qinghai, and was identified and preserved in the Guangdong Provincial Microbiological Collection Center (GDMCC) on December 3, 2024, address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, and the preservation number is: GDMCC No.65577.
[0025] The Cordyceps sinensis Naqu strain Ophiocordyceps sinensis XZNQ2206 of the present invention is isolated from Naqu, Tibet, and was identified and preserved in the Guangdong Provincial Microbiological Collection Center (GDMCC) on December 3, 2024, address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, and the preservation number is: GDMCC No.65578.
[0026] The Gansu strain of Cordyceps sinensis GS-MQ was isolated from Gansu and is currently a commonly used infection strain.
[0027] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.
[0028] Embodiment 1:
[0029] The Cordyceps sinensis culture medium used in this example contains 0.01% N-acetylglucosamine.
[0030] 1. Preparation of Cordyceps sinensis blastospores
[0031] The culture medium for blastospore cultivation was PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)), add 0.01% N-acetylglucosamine by mass, the preparation method is to mix the ingredients evenly, sterilize and set aside, the same below. After accurate molecular identification, the Cordyceps sinensis conidia are inoculated into the above PPDA medium and placed in a shaker (120rpm, 12℃) for 60 days. The cultured Cordyceps sinensis liquid (containing about 10 blastospores and hyphae per ml) is 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0032] At the same time, Cordyceps sinensis was cultured in PPDA medium (by mass fraction, 2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)) was placed in a shaker (120 rpm, 12°C) and cultured for 60 days. The cultured Cordyceps sinensis liquid (containing blastospores and hyphae at about 10 ml) was 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0033] 2. Rearing of small golden bat moth larvae
[0034] Carrots (cut into 1-3 cm pieces), Polygonum bulbilianum (cut into 1-3 cm pieces) and Potentilla anserina (uncut) were mixed and fed to bat moth larvae in a mass ratio of 1:1:1 to obtain healthy one-instar golden bat moth larvae for infection.
[0035] 3. Surface infection
[0036] The Cordyceps sinensis culture fluid (about 10 per ml) obtained from the two culture media was 6 blastospores) were diluted with sterile water to about 10 per ml 5 10 blastospores 5The amount of blastospores is added to 100 grams of sterile coconut bran (water content is about 65%), and then the coconut bran containing blastospores is mixed with 100 grams of bat moth larvae feed (Polygonum bulbiferum), and about 500 eggs are placed on the coconut bran mixed with feed. After the larvae hatch, they are infected with the Cordyceps sinensis fungus liquid cultured by the above method. The bat moth larvae infected with the body surface are placed at 11-16°C (carrots, Polygonum bulbiferum and Potentilla anserina are mixed and fed to the bat moth larvae in a mass ratio of 1:1:1).
[0037] 4. Detection of bacteria carried by bat moth larvae infected on the body surface
[0038] Three months after the infection, about 2-5 μl of hemolymph was collected from the abdomen of the larvae using a micro needle (needle diameter 0.06 mm). After staining with Calcofluor White (Sigma), the bacterial morphology was observed under a microscope, and a large number of blastospores were observed. The larvae carrying rate of the Cordyceps sinensis larvae reached 100% after 3 months of surface infection with the larvae of the small golden bat moth by the Yushu strain Ophiocordyceps sinensis QHYS2022-3 or the Nagqu strain Ophiocordyceps sinensis XZNQ2206 cultured in PPDA medium containing 0.01% acetylglucosamine. The larvae carrying rate of the Cordyceps sinensis GS-MQ Gansu strain cultured under the same conditions was about 29% after 3 months of surface infection with the small golden bat moth. The larvae carrying rate of the Cordyceps sinensis larvae of the Yushu strain, Nagqu strain or Gansu strain cultured in PPDA medium not containing acetylglucosamine was zero after 3 months of surface infection with the small golden bat moth larvae.
[0039] 5. Fruiting body induction
[0040] After 5-7 months of growth, the bat moth larvae infected by the Yushu strain or Naqu strain of Cordyceps sinensis cultured in PPDA medium containing 0.01% acetylglucosamine, changed from live larvae to dead larvae, the dead larvae's head cracked, and the fruiting bodies grew out from the cracked dead larvae's head. The dead larvae infected by the Yushu strain had a dead larvae rate of 51.5%, the dead larvae infected by the Naqu strain had a dead larvae rate of 49.5%, and the dead larvae infected by the Gansu strain had a dead larvae rate of 8.5%. The artificially cultivated Cordyceps sinensis with the same morphology as wild Cordyceps sinensis was obtained, and the fruiting body incidence rate of the Yushu strain, the Naqu strain, and the Gansu strain was 90.5%, 92.6%, and 8.5%. The bat moth larvae infected by the Yushu strain, Naqu strain, or Gansu strain of Cordyceps sinensis cultured in PPDA medium without acetylglucosamine had a dead larvae rate of zero because they did not carry Cordyceps sinensis.
[0041] Embodiment 2:
[0042] The Cordyceps sinensis culture medium used in this example contains 0.1% N-acetylglucosamine.
[0043] 1. Preparation of Cordyceps sinensis blastospores
[0044] The culture medium for blastospore cultivation was PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)), plus 0.1% N-acetylglucosamine. After accurate molecular identification, the conidia of Cordyceps sinensis were inoculated into the above PPDA medium and cultured in a shaker (120 rpm, 12°C) for 60 days. The cultured Cordyceps sinensis liquid (containing about 10 blastospores and hyphae per ml) was 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0045] At the same time, Cordyceps sinensis was cultured in PPDA medium (by mass fraction, 2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)) was placed in a shaker (120 rpm, 12°C) and cultured for 60 days. The cultured Cordyceps sinensis liquid (containing blastospores and hyphae at about 10 ml) was 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0046] 2. Rearing of small golden bat moth larvae
[0047] Carrot, Polygonum bulbiferum and Potentilla anserina were mixed and fed to bat moth larvae in a mass ratio of 1:1:1 to obtain healthy one-instar golden bat moth larvae for infection.
[0048] 3. Surface infection
[0049] The Cordyceps sinensis culture fluid (containing blastospores and hyphae at about 10 ml) obtained from the two culture media was 6 blastospores) were diluted with sterile water to about 10 per ml 5 10 blastospores 5The amount of blastospores was added to 100 grams of sterile coconut bran (water content of about 65%), and then the coconut bran containing blastospores was mixed with 100 grams of bat moth larvae feed (Polygonum bulbiferum), and about 500 eggs were placed on the coconut bran mixed with feed. After the larvae hatched, they were infected with Cordyceps sinensis cultured in the above method. The bat moth larvae infected with the body surface were placed at 11-16℃ (the bat moth larvae were fed with carrots, Polygonum bulbiferum and Potentilla anserina in a mass ratio of 1:1:1).
[0050] 4. Detection of bacteria carried by bat moth larvae infected on the body surface
[0051] Three months after the infection, about 2-5 μl of hemolymph was collected from the abdomen of the larvae using a micro needle (needle diameter 0.06 mm). After staining with Calcofluor White (Sigma), the bacterial morphology was observed under a microscope, and a large number of blastospores were observed. The larvae carrying rate of the Cordyceps sinensis larvae after 3 months of surface infection with the Cordyceps sinensis Yushu strain Ophiocordyceps sinensis QHYS2022-3 or the Nagqu strain Ophiocordyceps sinensis XZNQ2206 cultured in PPDA medium containing 0.1% acetylglucosamine reached 100%. The larvae carrying rate of the Cordyceps sinensis Gansu strain Ophiocordyceps sinensis GS-MQ cultured under the same conditions was about 42%; the larvae carrying rate of the Cordyceps sinensis Yushu strain, Nagqu strain or Gansu strain cultured in PPDA medium not containing acetylglucosamine was zero after 3 months of surface infection with the Cordyceps sinensis larvae.
[0052] 5. Fruiting body induction
[0053] After 5-7 months of growth, the bat moth larvae infected by the Yushu strain or Naqu strain of Cordyceps sinensis cultured in PPDA medium containing 0.1% acetylglucosamine, changed from live larvae to dead larvae, the dead larvae's head cracked, and the fruiting bodies grew out from the cracked dead larvae's head. The dead larvae infected by the Yushu strain had a dead larvae rate of 55.5%, the dead larvae infected by the Naqu strain had a dead larvae rate of 53.5%, and the dead larvae infected by the Gansu strain had a dead larvae rate of 10.5%. The artificially cultivated Cordyceps sinensis with the same morphology as wild Cordyceps sinensis was obtained, and the fruiting body incidence rate of the Yushu strain, the Naqu strain, and the Gansu strain was 12.5%. The bat moth larvae infected by the Yushu strain, the Naqu strain, or the Gansu strain of Cordyceps sinensis cultured in PPDA medium without acetylglucosamine had a dead larvae rate of zero because they did not carry Cordyceps sinensis.
[0054] Embodiment 3:
[0055] The Cordyceps sinensis culture medium used in this example contains 0.5% acetylglucosamine.
[0056] 1. Preparation of Cordyceps sinensis blastospores
[0057] The culture medium for blastospore cultivation was PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)), plus 0.5% N-acetylglucosamine. After accurate molecular identification, the conidia of Cordyceps sinensis were inoculated into the above PPDA medium and cultured in a shaker (120 rpm, 12°C) for 60 days. The cultured Cordyceps sinensis liquid (containing about 10 blastospores and hyphae per ml) was 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0058] At the same time, Cordyceps sinensis was cultured in PPDA medium (by mass fraction, 2% glucose, 20% potato juice, 1% peptone, 0.3% KH 2 PO 4 、0.15% MgSO 4 7H 2 O and 0.002% VB1, the rest is H 2 O (natural pH)) was placed in a shaker (120 rpm, 12°C) and cultured for 60 days. The cultured Cordyceps sinensis liquid (containing blastospores and hyphae at about 10 ml) was 6 blastospores) are used to infect the first instar larvae of the golden bat moth on the body surface.
[0059] 2. Rearing of small golden bat moth larvae
[0060] Carrot, Polygonum bulbiferum and Potentilla anserina were mixed and fed to bat moth larvae in a mass ratio of 1:1:1 to obtain healthy one-instar golden bat moth larvae for infection.
[0061] 3. Surface infection
[0062] The Cordyceps sinensis culture fluid (containing blastospores and hyphae at about 10 ml) obtained from the two culture media was 6 blastospores) were diluted with sterile water to about 10 per ml 5 10 blastospores 5The amount of blastospores is added to 100 grams of sterile coconut bran (water content is about 65%), and then the coconut bran containing blastospores is mixed with 100 grams of bat moth larvae feed (Polygonum bulbiferum), and about 500 eggs are placed on the coconut bran mixed with feed. After the larvae hatch, they are infected with the Cordyceps sinensis fungus liquid cultured by the above method. The bat moth larvae infected with the body surface are placed at 11-16°C (carrots, Polygonum bulbiferum and Potentilla anserina are mixed and fed to the bat moth larvae in a mass ratio of 1:1:1).
[0063] 4. Detection of bacteria carried by bat moth larvae infected on the body surface
[0064] Three months after the infection, about 2-5 μl of hemolymph was collected from the abdomen of the larvae using a micro needle (needle diameter 0.06 mm). After staining with Calcofluor White (Sigma), the bacterial morphology was observed under a microscope, and a large number of blastospores were observed. The larvae carrying rate of the Cordyceps sinensis larvae after being superficially infected with the small golden bat moth larvae by the Yushu strain Ophiocordyceps sinensis QHYS2022-3 or the Nagqu strain Ophiocordyceps sinensis XZNQ2206 cultured in PPDA medium containing 0.5% acetylglucosamine reached 100%. The larvae carrying rate of the Cordyceps sinensis GS-MQ Gansu strain Ophiocordyceps sinensis GS-MQ cultured under the same conditions was about 36% after being superficially infected with the small golden bat moth larvae for 3 months; the larvae carrying rate of the Cordyceps sinensis larvae after being superficially infected with the Yushu strain, Nagqu strain or Gansu strain of Cordyceps sinensis cultured in PPDA medium not containing acetylglucosamine was zero.
[0065] 5. Fruiting body induction
[0066] After 5-7 months of growth, the bat moth larvae infected by the Yushu strain or Naqu strain of Cordyceps sinensis cultured in PPDA medium containing 0.5% acetylglucosamine will change from live larvae to dead larvae, with the head of the dead larvae cracking and fruiting bodies growing out from the cracks in the head of the dead larvae. The dead larvae infected by the Yushu strain have a dead larvae rate of 50.5%, the dead larvae infected by the Naqu strain have a dead larvae rate of 47.5%, and the dead larvae infected by the Gansu strain have a dead larvae rate of 7.5%. The artificially cultivated Cordyceps sinensis with the same morphology as wild Cordyceps sinensis was obtained, with the fruiting body incidence rates of 89.5% for the Yushu strain, 90.4% for the Naqu strain, and 10.5% for the Gansu strain. The bat moth larvae infected by the Yushu strain or Naqu strain of Cordyceps sinensis cultured in PPDA medium without acetylglucosamine have a dead larvae rate of zero because they do not carry Cordyceps sinensis.
Claims
1. Application of N-acetylglucosamine in promoting the infection of Cordyceps sinensis in bat moth larvae, inducing Cordyceps sinensis to grow fruiting bodies in bat moth larvae, and increasing the dead insect rate of bat moth larvae.
2. The use according to claim 1, characterized in that: The N-acetyl glucosamine is placed in a culture medium of Cordyceps sinensis to cultivate Cordyceps sinensis, and then the bacterial solution is used to infect the bat moth larvae.
3. The use according to claim 1, characterized in that: The concentration of N-acetylglucosamine is 0.01-1% by mass, preferably 0.01-0.5% by mass.
4. The use according to claim 1, characterized in that: The cordyceps sinensis is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206. The Ophiocordyceps sinensis QHYS2022-3 has a preservation number of GDMCC No. 65577; the Ophiocordyceps sinensis XZNQ2206 has a preservation number of GDMCC No. 65578.
5. The use according to claim 1, characterized in that: The bat moth larvae are small golden bat moth larvae, preferably 1-5 instar larvae.
6. A method for artificially cultivating a new strain of Cordyceps sinensis, characterized in that: The following steps are involved: 1) adding 0.01-1% N-acetylglucosamine to a liquid culture medium of Cordyceps sinensis, and then inoculating Cordyceps sinensis for cultivation to obtain a mixture of blastospores and hyphae; 2) Infect bat moth larvae with the mixed liquid surface, and then culture them to obtain Cordyceps sinensis.
7. The method according to claim 6, characterized in that The cordyceps sinensis is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206. The Ophiocordyceps sinensis QHYS2022-3 has a preservation number of GDMCC No. 65577; the Ophiocordyceps sinensis XZNQ2206 has a preservation number of GDMCC No. 65578.
8. The method according to claim 6, characterized in that The bat moth larvae are small golden bat moth larvae, preferably 1-5 instar larvae.
9. The method according to claim 6, characterized in that The mixture is mixed with sterile coconut bran and then the body surface is infected with bat moth larvae.
10. Ophiocordyceps sinensis QHYS2022-3, deposit number: GDMCC No.65577; or Cordyceps sinensis XZNQ2206, deposit number: GDMCC No.65578.