Human calcified placenta acellular extracellular matrix and preparation method thereof

The risk of cysts and nodules caused by dilute hydrochloric acid is solved by removing the placental calcification points and decellularization and degassing treatment in a slightly alkaline environment. The risk of human placental decellularization extracellular matrix materials triggering cysts and nodules after implantation is solved, reducing the cost and time of sample selection, and improving the safety and application efficiency of the material.

CN119680011APending Publication Date: 2025-03-25广州远想医学生物技术有限公司
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Patent Information

Application Number
CN202411847810.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-16
Publication Date
2025-03-25

AI Technical Summary

Technical Problem

The extracellular matrix material of human placenta is prone to cause the risk of cysts and nodules after implantation, and the selection of suitable samples is expensive and time-consuming, and the lipid components in the placenta may lead to immune rejection.

Method used

The placental tissue fragments were soaked with dilute hydrochloric acid solution to remove calcification points, and decellularized and degreased using trypsin and SDS solutions in a slightly alkaline environment, followed by virus inactivation, freeze-drying and sterilization.

Benefits of technology

Effectively remove calcification points in the placenta, reduce the risk of cysts and nodules after implantation, reduce the cost and time of selecting samples, reduce fat content and DNA residues, and improve the safety and application efficiency of materials.

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Abstract

According to the human calcified placenta acellular matrix and the preparation method thereof, calcification points of placenta tissue fragments are removed by using a diluted hydrochloric acid solution, and then the acellular matrix is prepared. The prepared extracellular matrix does not contain calcification points, so that the risk that cysts and nodules are generated after the subsequently prepared human placenta acellular matrix is implanted is reduced; moreover, the cost and time for selecting a proper sample are reduced, so that the human placenta sample can be used up, and the waste of tissue resources is avoided.
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