Extremely trace extraction, enzymatic hydrolysis and salt removal integrated kit

Through the integrated kit of extremely micro-extraction enzyme desalination, combined with improved suspension capture technology and single-tube microreactor, the problems of high sample loss, high time cost and compatibility in the prior art are solved, and high sensitivity, low concentration protein analysis and efficient sample processing are achieved.

CN119708116APending Publication Date: 2025-03-28COMMPOBOAO (HANGZHOU) BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411947219.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-27
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The prior art has problems such as high sample loss, high time cost, compatibility problems and low micro sample processing efficiency when processing ultra-microscopic samples, which cannot meet the needs of high sensitivity analysis of low concentration proteins.

Method used

It provides an integrated kit for ultra-fine extraction of enzymatic desalination. Based on improved suspension capture technology, it realizes the integrated operation of sample extraction, enzymatic desalination and desalination. It uses a single-tube microreactor to integrate protein dissolution, enzymatic desalination and desalination, and combines ultra-micro-reaction system and high-performance liquid chromatography (LC-MS) to improve the recovery rate of low-abundance proteins and peptides.

Benefits of technology

It realizes high sensitivity and low concentration protein analysis, reduces sample loss and processing time, improves laboratory work efficiency, and is compatible with a variety of complex biological samples, suitable for high-throughput research needs.

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Abstract

The invention discloses an extremely trace extraction, enzymolysis and desalination integrated kit, and relates to the technical field of proteomics research, the kit is based on an improved suspension capture technology, and can realize integrated operation of sample extraction, enzymolysis and desalination. The technical scheme of the kit comprises the core steps of protein capturing and washing, integrated treatment, efficient enzymolysis and sensitivity improvement. The extremely trace extraction, enzymolysis and demineralization integrated kit has high sensitivity, can realize high repeatability and high detection coverage rate under the condition of a picogram-level sample size, avoids multiple transfer steps through single-tube operation, reduces sample loss, is simple and convenient to operate, can significantly reduce sample preparation steps and time consumption, and improves the working efficiency of a laboratory. Besides, the kit can be compatible with various complex biological samples including serum, tissue, cells and single cell samples, is wide in application range, can replace commercial expensive kits, and is particularly suitable for high-throughput research requirements.
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