Preparation method of glucagon-like peptide-1 analogue
Through the method of recombinant granulation and engineering strain construction, combined with the combination of CipA protein and Ssp DnaB, the protein purification process of GLP-1 is simplified, and the problems of low yield, high cost and environmental pollution in the prior art are solved, and efficient and economical preparation of GLP-1 is achieved.
CN119709807APending Publication Date: 2025-03-28绵阳晟氏健康科技有限公司
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Patent Information
- Application Number
- CN202411984623.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
In the prior art, the preparation method of GLP-1 analog has problems such as low yield, high cost and great environmental pollution.
Method used
The methods of recombinant granulation construction, engineering strain construction, inducible expression, fermentation culture and protein purification are used to simplify the protein purification steps, increase the yield of GLP-1, and reduce the cost of raw materials amino acids through the combination of CipA protein and Ssp DnaB.
Benefits of technology
It improves the yield of GLP-1, reduces the cost of raw materials, reduces the use of organic solvents in chemical synthesis, and reduces the pollution to the environment.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the technical field of biological pharmacy, and provides a preparation method of a glucagon-like peptide-1 analogue, which comprises the steps of recombinant granulation construction, engineering strain construction, inducible expression, fermentation culture and protein purification. According to the method, the protein purification step is simplified, and the combination mediation of the Ci pA protein and the small intein (synechocystis) is adopted, so that the yield of the GLP-1 is improved, the cost of the raw material amino acid is reduced, the use of an organic solvent in chemical synthesis is reduced, and the pollution to the environment is reduced.
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