STR genetic marker detection method based on spectrum correction matrix synchronous construction and interior label

By using composite internal standards embedded in spectral feature fragments in STR detection, the synchronous construction of the spectral correction matrix is ​​achieved, solving the problem of pre-establishing the spectral correction matrix in the prior art, simplifying the process and reducing costs.

CN119709978APending Publication Date: 2025-03-28THE FIRST RES INST OF MIN OF PUBLIC SECURITY
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Patent Information

Application Number
CN202411808889.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-10
Publication Date
2025-03-28

AI Technical Summary

Technical Problem

The existing STR detection methods require the pre-establishment of spectral correction matrix, which is complex in experimental processes and high maintenance costs.

Method used

The composite internal standard embedded in the spectral characteristic fragment is used to complete the synchronous construction of sample detection and spectral correction matrix through one electrophoresis, and the special electrophoresis link of spectral correction reagent is removed.

Benefits of technology

It realizes STR detection without pre-establishing the spectral correction matrix, simplifies the experimental process, reduces operating and maintenance costs, and ensures detection quality.

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Abstract

The invention discloses an STR (short tandem repeat) genetic marker detection method based on spectrum correction matrix synchronous construction and an internal label, and designs the STR genetic marker detection method based on spectrum correction matrix synchronous construction by innovatively adopting a composite internal label embedded with a spectrum characteristic fragment and a reconstruction spectrum correction mapping calculation method. And a special spectrum correction reagent electrophoresis link is removed, so that the STR genetic marker detection of the sample can be completed by one-time electrophoresis. By utilizing the device and the method, efficient STR detection can be realized, the problem that a spectrum correction matrix is not matched with actual STR electrophoresis after an instrument is used for a long time, an STR detection reagent is replaced, a light path is adjusted and a capillary array is replaced can be avoided, and the device and the method also play an important role in reducing the operation and maintenance cost and ensuring the detection quality of STR genetic markers.
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