Primer group, method and kit for detecting vibrio parahaemolyticus
By providing primer sets and multiple fluorescence probe PCR detection methods for Vibrio parahaemolytic detection, the problem of long detection time and difficulty in detecting specific genes and virulence genes in the prior art is solved, and rapid and accurate detection and distinction between virulence strains are achieved.
CN119710043APending Publication Date: 2025-03-28WEIHAI MUNICIPAL HOSPITAL +2
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Patent Information
- Application Number
- CN202411882315.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-19
- Publication Date
- 2025-03-28
AI Technical Summary
Technical Problem
In the prior art, Vibrio parahaemolyticus has a long detection time and is difficult to detect its specific genes and virulence genes simultaneously.
Method used
A primer set and multiple fluorescence probe PCR detection method for detection of Vibrio parahaemolyticus is provided, which can simultaneously amplify the specific genes gyrB, DAADH and virulence genes TDH, T3SS1-VopS, and T3SS2-VopT of Vibrio parahaemolyticus.
Benefits of technology
This method does not require bacterial culture and can quickly and accurately detect Vibrio parahaemolyticus in clinical specimens, and distinguish virulent strains from non-virulent strains, shorten the detection time and improve the detection efficiency.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention relates to a primer group, a method and a kit for detecting vibrio parahaemolyticus. The multiplex fluorescent quantitative PCR kit for detecting the vibrio parahaemolyticus comprises a primer group, a probe group and a PCR amplification premix solution. Wherein the primer group comprises at least one of specific genes gyrB and DAADH of vibrio parahaemolyticus and at least one of virulence genes TDH, T3SS1-VopS and T3SS2-VopT of vibrio parahaemolyticus, and the proportion of primers to probes in a PCR (Polymerase Chain Reaction) system is optimized. When the kit disclosed by the invention is used for detecting vibrio parahaemolyticus, microbial isolated culture and identification are not needed, so that the detection time is shortened, the detection efficiency is improved, meanwhile, the accuracy, sensitivity, stability and specificity of detection are greatly improved, and the kit has huge advantages in clinical specimen detection and epidemiological research.
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