Constant-temperature water bath culture device for multi-gas flux measurement

By designing a constant temperature water bath culture device for multi-gas flux measurement, the problem of difficulty in accurately measuring the gas emission flux generated by denitrification in the soil in the prior art is solved, and continuous measurement of N2O, N2, and CO2 is achieved, improving the accuracy of measurement and the growth environment of microorganisms.

CN119738507APending Publication Date: 2025-04-01LANZHOU UNIV +1
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Patent Information

Application Number
CN202510252967.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-05
Publication Date
2025-04-01

AI Technical Summary

Technical Problem

The prior art is difficult to accurately measure the emission flux of N2 and N2O generated by denitrification in soil, and the opaque material and small size of the device hinder the proliferation of microorganisms and affect the detection of gas flux.

Method used

A constant temperature water bath culture device for multi-gas flux measurement is designed, including a transparent constant temperature water bath base chamber, a plexiglass cover, a culture pot, an air flow path control device and a meteorological chromatograph. The gas storage device and a helium flushing water tank are connected through a conduit to achieve continuous measurement of N2O, N2 and CO2.

Benefits of technology

The continuous measurement of the flux of N2O, N2, and CO2 within a few days or weeks of plant cultivation is achieved, which improves the accuracy of measurement and the growth environment of microorganisms, and avoids the adverse effects of device size and material on detection.

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Abstract

The invention discloses a constant-temperature water bath culture device for multi-gas flux measurement, and belongs to the technical field of agricultural detection, the constant-temperature water bath culture device comprises a culture combination box, a transparent constant-temperature water bath base chamber is arranged in the culture combination box, and a plurality of culture pots are arranged in the transparent constant-temperature water bath base chamber; the transparent constant-temperature water bath base chamber is connected with an airflow path control device through a pipeline, and the airflow path control device is connected with a gas chromatograph through a pipeline; a resin glass guide pipe connector is arranged at the top of the resin culture glass cover and is in pipeline connection with a helium flushing water tank; the transparent constant-temperature water bath base chamber and the resin glass guide pipe connector are connected with a gas storage device through pipelines; and a light source is arranged at the top in the culture combination box. According to the invention, the flux of N2O, N2 and CO2 can be continuously measured at the same time within several days or weeks of plant cultivation.
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