Soothing composition for relieving itching of body and application thereof

Abstract

The invention discloses a soothing composition for relieving itching of a body and application of the soothing composition, and relates to the technical field of cosmetics. The invention discloses a soothing and itching relieving composition, which is prepared from the following ingredients in parts by weight: 0.1 to 0.5 part of phoenix dactylifera seeds, 1 to 5 parts of hyperforin perforatum flower extracts, 1 to 10 parts of Brazil nectarine fruit oil and 0.01 to 0.2 part of L-homoserine. Through cooperation of the phoenix dactylifera seeds, the hyperforin perforatum flower extract, the Brazilian nectarine fruit oil and the L-homoserine, on one hand, when the temperature changes and nursing is improper, sensitive skin can be helped to be calm and relaxed, and the resistance of the skin to external environment influence is enhanced; on the other hand, the composition can synergistically moisturize, relieve and reduce skin sensitivity and prevent skin itch and dryness.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a soothing composition for relieving itching on the body and application thereof. Background Art

[0002] Itch is a complex sensation that involves the activation of specific physiological and cellular receptors. The sensory nerves extend to the epidermis of the skin, and external stimuli can easily reach the nerves between them and release the itch factor Substance P (SP). SP binds to the NK-1 receptor of mast cells and releases substances such as histamine, among which histamine causes itching by stimulating its cognate receptor (H1 receptor) and subsequently activating the TRPV1 ion channel in the sensory neurons. It is common in people with sensitive skin, especially dry and sensitive skin. Dry skin is caused by skin barrier dysfunction caused by environmental factors (such as exposure to sunlight, temperature and humidity). In general, dry skin can induce chronic itching, which is a common clinical symptom of skin diseases. Excessive cleaning can cause damage to the skin barrier. Some surfactants act and remain in the stratum corneum of the skin, and the damage to the keratinocyte membrane structure is irreversible, causing irritation, causing skin itching, rashes, and dermatitis.

[0003] The products currently used to relieve itching include glucocorticoids, calamine lotion, calcineurin inhibitors, etc., but these pose great risks to people with sensitive skin and can easily cause skin thinning and atrophy. Some products are also difficult to clean and remain on the skin, causing irritation that is not good for skin health. Summary of the invention

[0004] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a soothing and antipruritic composition with soothing, moisturizing, antipruritic and barrier repair effects, as well as a preparation method and application thereof.

[0005] To achieve the above objectives, in a first aspect of the present invention, the present invention provides a soothing and antipruritic composition, comprising the following components in parts by weight: 0.1-0.5 parts of date palm seeds, 1-5 parts of Hypericum perforatum flower extract, 1-10 parts of nectarine oil, and 0.01-0.2 parts of L-homoserine.

[0006] The present invention prepares a soothing and antipruritic composition with soothing, moisturizing, antipruritic and barrier repair effects by synergizing specific weight portions of date palm seeds, Hypericum perforatum flower extract, Brazilian nectarine wood oil and L-homoserine, and the composition is suitable for sensitive skin.

[0007] Phoenix dactylifera seeds, also known as date pits, are the seeds of the fruit produced by the date palm tree (also known as the date palm). Date seeds contain a large amount of flavonoids, such as quercetin, rutin, etc., as well as polyphenols, vitamin E, phytosterols, etc. These ingredients have strong antioxidant effects, can fight free radicals, reduce cell damage, and have anti-aging, anti-inflammatory, and anti-allergic effects. Hypericum perforatum L belongs to the family of Garcinia. Modern chemical analysis shows that its main components include 6 anthraquinone compounds; 7 flavonoid compounds; 35 terpenoid compounds; a small amount of cinnamic acid derivatives and vanillic acid and other chemical components. Among them, the anthraquinone compound hypericin has obvious pharmacological effects in antiviral, antidepressant, and anti-tumor. When used together, date seed and Hypericum perforatum flower extracts can enhance each other's effectiveness, producing a synergistic anti-inflammatory and antioxidant effect. They can also help calm and soothe sensitive skin during temperature changes and improper care, and enhance the skin's resistance to external environmental influences.

[0008] Caryocar brasiliense oil is a vegetable oil extracted from the fruit of the Brazilian nectarine tree (Caryocar brasiliense). The fatty acid composition of Caryocar brasiliense oil is very rich, especially saturated fatty acids such as oleic acid and palmitic acid. Oleic acid accounts for about 40%-60%, which helps to deeply moisturize and repair the skin; as well as polyunsaturated fatty acids such as linoleic acid, which has a good moisturizing effect on the skin, can strengthen the skin barrier and reduce water loss. L-homoserine is a non-essential amino acid with a chiral carbon. It is not only a precursor compound for the synthesis of L-threonine and L-methionine, but also can synthesize valuable compounds such as O-acetyl homoserine, O-succinyl-L-homoserine, 1,3-propanediol and isobutanol. Its amino and carboxyl structures make it hydrophilic, which enables L-homoserine to bind to water molecules, thus having good moisturizing properties. In the composition provided by the present invention, L-homoserine provides hydration, while Brazilian nectarine wood oil provides moisturizing and deep repair. The combination of the two can help the skin maintain an ideal water-oil balance, lock in moisture, and prevent moisture loss, ensuring long-term moisturizing and comfort of the skin. This combination is particularly suitable for sensitive and inflammatory skin. Therefore, Brazilian nectarine wood oil and L-homoserine can synergistically moisturize, soothe, reduce skin sensitivity, promote skin smoothness, prevent skin itching and dryness,

[0009] The present invention uses date palm seeds, Hypericum perforatum flower extract, Brazilian nectarine oil and L-homoserine to, on the one hand, help calm and soothe sensitive skin when the temperature changes and care is inappropriate, and enhance the skin's resistance to the influence of the external environment; on the other hand, it can synergistically moisturize and soothe, reduce skin sensitivity, and prevent skin itching and dryness.

[0010] Preferably, the soothing and antipruritic composition comprises the following components in parts by weight: 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of nectarine oil, and 0.05-0.1 parts of L-homoserine.

[0011] Preferably, in the soothing and antipruritic composition, the total mass percentage of oleifera oleifera oil and L-homoserine is 65-75%.

[0012] The inventors found in actual research that the weight of the components in the soothing and antipruritic composition will affect the performance of the product. When the weight of the components is further selected to be within the above range, the obtained product has better soothing, moisturizing, antipruritic and barrier repair effects.

[0013] Preferably, the mass percentage of hypericin in the Hypericum perforatum flower extract is 0.1-0.3%.

[0014] Preferably, the mass percentage of hypericin in the Hypericum perforatum flower extract is 0.14-0.2%.

[0015] The inventors found in actual research that the mass percentage of hypericin in the Hypericum perforatum flower extract has a great influence on the antipruritic effect of the final soothing and antipruritic composition. When the mass percentage of hypericin in the Hypericum perforatum flower extract is 0.14-0.2%, the antipruritic effect is better.

[0016] In the second aspect of the present invention, the present invention provides a preparation method of a Hypericum perforatum flower extract as follows: crushing and sieving the Hypericum perforatum flowers to obtain Hypericum perforatum flower powder, mixing it with an ethanol solution and protecting it from light, rotating and oscillating, centrifuging, collecting the supernatant, concentrating the supernatant and drying it to obtain the Hypericum perforatum flower extract.

[0017] Preferably, the volume fraction of the ethanol is 50-95%, and the weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 8-12:1.

[0018] Preferably, the rotation speed of the rotary oscillation is 100-150 r / min, and the rotation time of the rotary oscillation is 48-72 h; and / or, the centrifugal speed is 4000-6000 r / min, the centrifugal temperature is 2-6° C., and the centrifugal time is 5-15 min.

[0019] Preferably, the concentration temperature is 45-55°C.

[0020] More preferably, the volume fraction of the ethanol is 50-65%.

[0021] In the third aspect of the present invention, the present invention provides the use of the soothing and antipruritic composition in the preparation of skin products.

[0022] In a fourth aspect of the present invention, the present invention provides a skin product, comprising the soothing and antipruritic composition.

[0023] Preferably, the skin product includes any one of lotion, emulsion, cream, mask, essence and spray.

[0024] Preferably, the skin product comprises the following components in percentage by mass: 1-5% of the soothing and antipruritic composition, 4-40% of the cosmetic base, and the balance of water.

[0025] Preferably, the cosmetic matrix includes at least one of a thickener, a moisturizer, an emulsifier, a skin conditioner, an antioxidant, an emollient, a preservative, a fragrance, and a pH adjuster.

[0026] Preferably, the present invention provides a body lotion comprising the following components in percentage by mass: 1-5% soothing and antipruritic composition, 1-10% moisturizer, 0.1-0.5% thickener, 0.5-5% emulsifier, 0.05-1% skin conditioner, 0.5-1% antioxidant, 2-12% emollient, and the balance deionized water.

[0027] Exemplarily, the moisturizer includes at least one of glycerin, butylene glycol, low molecular weight sodium hyaluronate, and 1,2-hexanediol; the thickener includes at least one of xanthan gum, carbomer 980, high molecular weight cellulose, and polyacrylate cross-linked polymer-6; the emulsifier includes at least one of C12-20 alkyl glucoside, C14-22 alcohol, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, arachidyl alcohol, behenyl alcohol, and arachidyl glucoside; the skin conditioner includes at least one of trehalose and allantoin; the antioxidant includes at least one of p-hydroxyacetophenone and tocopheryl acetate; the emollient includes at least one of polydimethylsiloxane, avocado tree (BUTYROSPERMUM PARKII) fruit butter, caprylic / capric triglyceride, petrolatum, and stearyl alcohol.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention uses the synergistic effects of date palm seeds, Hypericum perforatum flower extract, Brazilian nectarine oil, and L-homoserine to, on the one hand, help calm and soothe sensitive skin when the temperature changes and care is inappropriate, and enhance the skin's resistance to the influence of the external environment; on the other hand, it can synergistically moisturize and soothe, reduce skin sensitivity, and prevent skin itching and dryness. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Graphs showing the results of the keratinocyte viability test in performance test 1, wherein (a) is a graph showing the results of the cell viability test in the blank control group, (b) is a graph showing the results of the cell viability test in Example 1, and (c) is a graph showing the results of the cell viability test in Comparative Example 1;

[0030] Figure 2 Graphs showing the immunofluorescence detection results of TRPV1 in performance test 2, wherein (d) is a graph showing the immunofluorescence detection results of TRPV1 in the blank control group, (e) is a graph showing the immunofluorescence detection results of TRPV1 in the negative control group, (f) is a graph showing the immunofluorescence detection results of TRPV1 in Example 1, and (g) is a graph showing the immunofluorescence detection results of TRPV1 in Comparative Example 1;

[0031] Figure 3 These are the scale test result diagrams in performance test 5, where (h) is the scale test result diagram of the blank application example, (i) is the scale test result diagram of application example 1, and (j) is the scale test result diagram of the comparative application example 1. DETAILED DESCRIPTION

[0032] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0033] The raw materials used in the examples and comparative examples of the present invention are described below, but are not limited to the following raw materials:

[0034] Date seeds: The product name is IBR- Purchased from Lucas Meyer Cosmetics;

[0035] Brazilian nectarine oil: The product name is Purchased from Mingliuji Electronic Trading Company in Baiyun District, Guangzhou;

[0036] L-Homoserine: Product Name Evermild TM L-HS, purchased from Shengde Biotech Co., Ltd.;

[0037] Brazil nut seed extract: The product name is Ethience Protect, purchased from Lucas Meyer Cosmetics;

[0038] Sea buckthorn fruit oil: model 7012, purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd.;

[0039] L-serine: model CDAA-270014, purchased from Shanghai Anpu Experimental Technology Co., Ltd.;

[0040] Hypericum perforatum flower extract-1: Homemade, the preparation method is as follows: (1) Crush the Hypericum perforatum flower and pass it through a 0.177 mm sieve to obtain the Hypericum perforatum flower powder, mix it with a 50% by volume ethanol solution, seal it, and protect it from light (dark conditions) at room temperature; the weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 8:1; (2) Rotate and oscillate the mixed solution, centrifuge it, collect the supernatant, and concentrate it by rotary evaporation at 45°C. After concentration, place it in an ultra-low temperature freeze dryer for drying and freezing to obtain the Hypericum perforatum flower extract; the rotation speed of the rotary oscillation is 120 r / min, and the rotation time is 72 h; the centrifugal speed is 5000 r / min, the centrifugal temperature is 4°C, and the centrifugal time is 10 min.

[0041] Hypericum perforatum flower extract-2: Homemade, the preparation method is the same as that of Hypericum perforatum flower extract-1, except that the volume fraction and dosage of the ethanol solution are different, and the rest of the preparation method is exactly the same; the Hypericum perforatum flower powder is mixed with a 60% volume fraction ethanol solution and then sealed, and kept away from light at room temperature (dark conditions), and the weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 10:1.

[0042] Hypericum perforatum flower extract-3: Homemade, the preparation method is the same as that of Hypericum perforatum flower extract-1, except that the volume fraction and dosage of the ethanol solution are different, and the rest of the preparation method is exactly the same; the Hypericum perforatum flower powder is mixed with a 70% ethanol solution by volume and then sealed, and kept away from light at room temperature (dark conditions), and the weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 10:1.

[0043] Hypericum perforatum flower extract-4: Homemade. The preparation method is the same as that of Hypericum perforatum flower extract-1, except that the volume fraction and dosage of the ethanol solution are different. The rest of the preparation method is exactly the same. The Hypericum perforatum flower powder is mixed with a 95% ethanol solution by volume and then sealed. The mixture is kept away from light at room temperature (dark conditions). The weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 12:1.

[0044] The content of hypericin in the Hypericum perforatum flower extracts 1-4 was determined by HPLC, and the hypericin contents of the Hypericum perforatum flower extracts 1-4 were 0.14%, 0.18%, 0.25% and 0.11%, respectively.

[0045] Examples and Comparative Examples

[0046] The embodiments of the present invention and the comparative examples provide a soothing and antipruritic composition, the components and weight portions of the soothing and antipruritic composition are selected as shown in Tables 1 and 2, and the preparation method of the soothing and antipruritic composition comprises the following steps: uniformly mixing date palm seeds, Hypericum perforatum flower extract, Brazilian nectarine wood oil, and L-homoserine with ultrasound to obtain the soothing and antipruritic composition; if the comparative example does not contain the relevant components, then no relevant components are added, and if there are replacement components, they can be mixed and added together.

[0047] Table 1

[0048]

[0049]

[0050] Table 2

[0051]

[0052] Application Examples

[0053] The application examples of the present invention provide a body lotion, the components (mass percentage) of the body lotion are shown in Table 3; wherein the soothing and antipruritic compositions in Application Examples 1-8 are respectively the soothing and antipruritic compositions prepared in Examples 1-8; the soothing and antipruritic compositions in Comparative Application Examples 1-6 are respectively the soothing and antipruritic compositions prepared in Comparative Examples 1-6; the blank application examples do not contain only the soothing and antipruritic composition; for example, the soothing and antipruritic composition used in Application Example 1 is the soothing and antipruritic composition in Example 1, the soothing and antipruritic composition used in Application Example 5 is the soothing and antipruritic composition in Example 5, the soothing and antipruritic composition used in Application Example 8 is the soothing and antipruritic composition in Example 8, and the same applies to the others; the soothing and antipruritic composition used in Application Example 9 is the soothing and antipruritic composition prepared in Example 1.

[0054] Table 3

[0055]

[0056] The preparation method of the body lotion provided in Application Example 1 is:

[0057] (1) Component A and water are mixed and stirred, heated to 80° C., homogenized at a speed of 1200 rpm for 5 minutes, and kept warm for later use to obtain prefabricated component A;

[0058] (2) Mixing component B, heating to 80° C., homogenizing at 1200 rpm for 5 min, and keeping warm for later use to obtain prefabricated component B;

[0059] (3) mixing the C components and heating them to 60° C. to melt them to obtain a prefabricated C component;

[0060] (4) The preformed component A is heated to 80°C, and the preformed component B is added at a rotation speed of 300 rpm, and stirred and mixed. Then, the temperature is lowered to 60°C, and the preformed component C is added at a rotation speed of 300 rpm and stirred and mixed. Subsequently, the temperature is lowered to below 45°C, and the soothing and antipruritic composition is added, and stirring is continued for 5 minutes to obtain an emulsion.

[0061] The preparation methods of the remaining application examples, comparative application examples, and blank application examples are the same as that of application example 1, except that no relevant components are added if they are not present.

[0062] Performance Test-1 Cell Activity Test

[0063] The effects of the soothing and antipruritic compositions prepared in the examples and comparative examples on cell viability were investigated.

[0064] Experimental materials: phosphate buffered saline (PBS), Shanghai Solebold; Shanghai Solebold; MTT, Yuanye Biotechnology; dimethyl sulfoxide (DMSO), Tianjin Damao; high glucose medium 1×DMEM, Gibco; fetal bovine serum (FBS), Gibco; keratinocytes were purchased from Guangzhou Customs Technology Center.

[0065] Experimental steps: The cell suspension was inoculated into a 96-well cell culture plate and returned to the incubator for 24 hours. The original culture medium in the well was discarded, and 100 μL of the test sample (the soothing and antipruritic composition was dissolved with DMSO to a final concentration of 0.06%) was added to each well of the sample group, and returned to the incubator for 24 hours. The culture medium was added to the blank control group. The culture plate was taken out, 20 μL of MTT solution was added to each well, and the incubator was incubated for 3-4 hours. The liquid in the well was removed, 100 μL of DMSO was added to each well, and after being placed on an oscillator for 10-15 minutes, the absorbance OD value at a wavelength of 570 nm on the microplate reader was measured, and the cell viability was calculated. The experimental data were expressed as mean ± SD, and the T test was used for data comparison and analysis. Compared with the blank control, it is indicated by *, *P<0.05, significant; **P<0.01, significantly significant; ***P<0.001, very significantly significant.

[0066] Cell viability (%) = (OD value of sample well - OD value adjusted to zero) / (OD value of blank control well - OD value adjusted to zero) × 100%.

[0067] The test results are shown in Table 4.

[0068] Table 4

[0069] sample Cell viability (%) Blank control group 100.00 Example 1 115.53** Example 2 114.87** Example 3 114.90** Example 4 114.69** Example 5 115.21** Example 6 104.27* Example 7 104.11* Example 8 110.24* Comparative Example 1 101.19 Comparative Example 2 100.82 Comparative Example 3 100.98 Comparative Example 4 99.98 Comparative Example 5 100.11 Comparative Example 6 100.07

[0070] Figure 1 The figures are the results of keratinocyte viability test, wherein (a) is the result of cell viability test of the blank control group, (b) is the result of cell viability test of Example 1, and (c) is the result of cell viability test of Comparative Example 1; Example 1 of the present invention can well enhance cell viability.

[0071] As can be seen from the above table, the soothing and antipruritic composition prepared in the embodiment of the present invention has a good effect of improving cell viability. As can be seen from the comparison of Examples 1-4, the mass percentage of hypericin in the Hypericum perforatum flower extract has little difference in effect on cell viability.

[0072] From the comparison of Example 1 and Examples 5-8, it can be seen that in the soothing and antipruritic composition, when the following weight portions are selected, i.e. 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of nut oil, and 0.05-0.1 parts of L-homoserine, the effect of improving cell viability is better. In the soothing and antipruritic composition, when the total mass percentage of nut oil and L-homoserine is 65-75%, the effect of improving cell viability is better.

[0073] Comparative Examples 1-6 lack some components or are not the synergistic components of the present invention, including date palm seeds, Hypericum perforatum flower extract, nectarine oil, and L-homoserine. From the results, it can be seen that the effects on cell viability are not significant.

[0074] Performance Test-2TRPV1 Inhibition Test

[0075] The present invention verifies the inhibitory effect of the soothing and antipruritic composition prepared in the examples and comparative examples on the expression of TRPV1. TRPV1 is a non-selective cation channel that is instantaneously open and can be activated by heat, weak acid, capsaicin and some endogenous ligands. In the skin, the TRPV1 channel is first identified on the nociceptive sensory nerve endings and is involved in many sensory afferent functions (such as pain, itching, temperature, chemical stimulation) and local (neurogenic inflammation released by various neuropeptides) and systemic (releasing analgesic neuropeptides) efferent functions. TRPV1 and histamine receptors are co-expressed in sensory afferent fibers. Studies have shown that inhibitors of TRPV1 can reduce the influx of calcium ions caused by histamine on DRG neurons, thereby alleviating the generation of itching. Specifically including the following contents:

[0076] (1) Grouping information: divided into a sample group (the soothing and antipruritic composition in the examples and comparative examples), a positive control group (PC, novanillin), a negative control group (NC), and a blank control group (BC);

[0077] (2) Experimental steps: epidermal skin model Transfer to the well plate, add EpiGrowth culture medium in advance, mark the test group number on the well plate, and test three groups in parallel. The BC group does not receive any treatment. The NC group, PC group and sample group add EpiGrowth culture medium containing 15 μM capsaicin CAP. The PC group and sample group add sample working solution of corresponding concentration (the concentration of the PC group is adjusted to 15.6 μg / mL with culture medium, and the volume concentration of the sample working solution group is 0.015% of the culture medium). The samples are evenly distributed on the surface of the model and placed in a CO2 incubator (37°C, 5% CO2) for incubation for 24 hours. After the incubation, the test substance remaining on the surface of the model is cleaned with a sterile PBS solution, and the residual liquid inside and outside the model is wiped with a sterile cotton swab.

[0078] (3) TRPV1 detection: Fix the cells with 4% paraformaldehyde for 30 min, perform immunofluorescence detection of capsaicin receptor TRPV1, observe under a microscope, collect images for analysis and record the optical density (IOD);

[0079] (4) Statistical analysis of results: The results were expressed as Mean ± SD. The t-test was used for comparison among the groups. *P < 0.05 was considered to be a significant difference, and **P < 0.01 was considered to be a very significant difference.

[0080] The calculation method of inhibition rate is: inhibition rate (%) = (average relative IOD value of negative control group - average relative IOD value of sample group) / average relative IOD value of negative control group × 100%;

[0081] The relative IOD refers to the accumulated optical density in each reaction well at each measurement, which is a relative value without unit; the relative IOD average refers to the average relative IOD value obtained from each measurement of three parallel reaction wells.

[0082] The test results are shown in Table 5.

[0083] Table 5

[0084]

[0085]

[0086] Figure 2 : are immunofluorescence detection result graphs of TRPV1, wherein (d) is the immunofluorescence detection result graph of TRPV1 in the blank control group, (e) is the immunofluorescence detection result graph of TRPV1 in the negative control group, (f) is the immunofluorescence detection result graph of TRPV1 in Example 1, and (g) is the immunofluorescence detection result graph of TRPV1 in Comparative Example 1. The soothing and antipruritic composition prepared in Example 1 of the present invention has a significantly better inhibition rate on TRPV1 expression.

[0087] As can be seen from the above table, the soothing and antipruritic composition prepared in the embodiment of the present invention has an inhibition rate of more than 40% on TRPV1 expression. As can be seen from the comparison of Examples 1-4, when the mass percentage of hypericin in the Hypericum perforatum flower extract is 0.14-0.2%, the inhibition rate on TRPV1 expression is higher and the antipruritic effect is better.

[0088] From the comparison of Example 1 and Examples 5-8, it can be seen that in the soothing and antipruritic composition, when the following weight portions are selected, namely 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of Brazilian nectarine wood fruit oil, and 0.05-0.1 parts of L-homoserine, the inhibition rate of TRPV1 expression is higher and the antipruritic effect is better. In the soothing and antipruritic composition, when the total mass percentage of Brazilian nectarine wood fruit oil and L-homoserine is 65-75%, the inhibition rate of TRPV1 expression is higher and the antipruritic effect is better.

[0089] Comparative Examples 1-6 lack some components or are not the synergistic components of the present invention, including date palm seeds, Hypericum perforatum flower extract, nectarine oil, and L-homoserine. As can be seen from the results, the antipruritic effect is poor.

[0090] Performance test-3 inflammatory factor inhibition test

[0091] Experimental materials: The cell line used was mouse macrophage RAW264.7, purchased from the Cell Resource Center of the Institute of Basic Medicine, Chinese Academy of Medical Sciences, fetal bovine serum (FBS, Gibco), DMEM high glucose medium (DMEM, Gibco), dimethyl sulfoxide (DMSO, Sigma), phosphate buffered saline (PBS, Gibco), thiazolyl blue (MTT, Sigma), and mouse interleukin-1β enzyme-linked immunosorbent assay kit (Mouse IL-1β ELISA kit, Boster).

[0092] Experimental steps: cells were seeded into 24-well plates and incubated in a 37°C, 5% CO2 incubator for 24 h;

[0093] According to the experimental grouping and concentration setting in Table 6, after the cells were plated and grown in the 24-well plate for 24 hours, they were grouped and sampled, and 3 replicate wells were set for each treatment group. Cell culture medium was added to the blank control and negative control groups, and cell culture medium containing samples of corresponding concentrations was added to the sample group, and cultured in a 37°C, 5% CO2 incubator for 24 hours;

[0094] The culture medium in the plate was aspirated, and PBS was added to wash once. Cell culture medium was added to the blank control group, and cell culture medium containing lipopolysaccharide (LPS) was added to the negative control group, sample group and positive control group. The supernatant was collected after continuing to culture in an incubator at 37°C and 5% CO2 for 24 hours. The cell supernatant of each well was taken, and the content of cellular inflammatory factors was detected according to the operating instructions of the ELISA kit; the data obtained in the experiment were processed and plotted using Excel software. SPSS17.0 was used for statistical analysis, and one-way analysis of variance (ANOVA) was used for inter-group comparison. When P<0.05, the difference was judged to be significant. The results are shown in Table 7. The inhibition rate was calculated as follows: Inhibition rate (%) = (average concentration of negative control group - average concentration of sample group) / average concentration of negative control group × 100%;

[0095] Table 6

[0096]

[0097] Table 7

[0098]

[0099]

[0100] As can be seen from the above table, the soothing and antipruritic composition prepared in the embodiment of the present invention has a good anti-inflammatory effect. As can be seen from the comparison of Examples 1-4, the mass percentage of hypericin in the Hypericum perforatum flower extract has little difference in the effect on the anti-inflammatory effect.

[0101] From the comparison of Example 1 and Examples 5-8, it can be seen that in the soothing and antipruritic composition, when the following weight portions are selected, namely 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of nut oil, and 0.05-0.1 parts of L-homoserine, the anti-inflammatory effect is better. In the soothing and antipruritic composition, when the total mass percentage of nut oil and L-homoserine is 65-75%, the anti-inflammatory effect is better.

[0102] Comparative Examples 1-6 lack some components or are not the specific date palm seeds, Hypericum perforatum flower extract, Brazilian nectarine oil, and L-homoserine synergistically of the present invention. From the results, the anti-inflammatory effect is poor.

[0103] Performance Test-4 Mildness Test

[0104] The present invention verifies the mildness of the application example lotion on human skin. According to the human skin patch test "Cosmetic Safety Technical Specifications" (2015), the test recruited 33 volunteers aged 18-60 years old, and the test environment was temperature (21±1)℃ and humidity (50±10)%. The volunteers washed their forearms with clean water, and after 5-10 minutes, after the water in the forearms evaporated, the tester applied 0.020-0.025mL of each group of sample lotions to each chamber of the 10-hole patch tester, selected the inner side of the two forearms of the volunteers, avoided the skin of the front and back hand joints, and each arm was attached with a sample patch tester, and marked accordingly. The patch time lasted for 24 hours, the patch tester was removed and the residual sample was wiped, and the skin reaction of 0.5h, 24h, and 48h was observed, and the results were recorded according to the skin reaction grading standard in the "Cosmetic Safety Technical Specifications" (2015). As shown in Table 8, the skin reaction is divided into 4 scoring levels, and the higher the score, the higher the severity of the skin irritation reaction.

[0105] Table 8

[0106]

[0107] After testing, the body lotions prepared in the application examples of the present invention, the comparative application examples and the blank application examples all showed negative reactions after human patch tests, and were safe and non-irritating to human skin.

[0108] Performance test-5 Human skin repair and moisturizing effects

[0109] The present invention verifies the efficacy of application examples, comparative application examples and blank application examples applied to human bodies, specifically including the following methods: According to the "Technical Specifications for Safety of Cosmetics" (2015), subjects aged 18-60 with frequent body itching are selected. Under normal circumstances, the subjects are randomly tested, with 5 people in each group, for a total of 16 groups. The products are continuously used for 28 days in a December winter environment, and instrument detection and clinical evaluation of skin indicators are performed on the 0th, 7th, 14th and 28th days of the test.

[0110] The specific steps of instrument measurement are as follows: After bathing every evening, the subjects take 1.5mL of body lotion and apply it to the forward bending area of ​​the arms, and massage it for absorption. On the day of the visit, the subjects cleaned with clean water without applying any products, and sat quietly in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The skin moisture content probe Corneometer (CM 825, Courage and Khazaka) was used to measure the MMV value before and after using the sample; the transepidermal water loss probe TewaMeter (TMHex, Courage+Khazaka) was used to measure the TEWL value before and after using the sample; and the VC 20Plus was used to test the scale index.

[0111] The data were recorded and the improvement rate of the index on the 28th day was calculated, where the improvement rate of the index = (value before use - value after use) / value before use × 100%. The test results are shown in Table 9.

[0112] Table 9

[0113]

[0114] Figure 3 The scale test results are shown in Figure 1, where (o) is the scale test result of the blank application example, (p) is the scale test result of application example 1, and (q) is the scale test result of comparative application example 1. The scale condition is significantly improved after using the body lotion of application example 1 of the present invention.

[0115] As can be seen from the above table, the body lotion prepared by the application example of the present invention has good repairing and moisturizing effects, and the scaling condition is significantly improved after use. As can be seen from the comparison of Examples 1-4, the mass percentage of hypericin in the Hypericum perforatum flower extract has little difference in the repairing and moisturizing effects, and the scaling condition after use.

[0116] From the comparison of Example 1 and Examples 5-8, it can be seen that in the soothing and antipruritic composition, when the following weight parts are selected, that is, 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of Brazilian nectarine wood fruit oil, and 0.05-0.1 parts of L-homoserine, the repair and moisturizing effects are better, and the scale condition is significantly improved after use. In the soothing and antipruritic composition, when the total mass percentage of Brazilian nectarine wood fruit oil and L-homoserine is 65-75%, the repair and moisturizing effects are better, and the scale condition is significantly improved after use.

[0117] Comparative Examples 1-6 lack some components or are not the synergistic components of the present invention, including date palm seeds, Hypericum perforatum flower extract, nectarine oil, and L-homoserine. From the results, the repair and moisturizing effects are poor, and the scaling condition is not significantly improved after use.

[0118] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A soothing and antipruritic composition, characterized in that: The invention comprises the following components in parts by weight: 0.1-0.5 parts of date palm seeds, 1-5 parts of Hypericum perforatum flower extract, 1-10 parts of Brazilian nectarine wood oil and 0.01-0.2 parts of L-homoserine.

2. The soothing and antipruritic composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 0.3-0.4 parts of date palm seeds, 2-3 parts of Hypericum perforatum flower extract, 5-8 parts of Brazilian nectarine wood oil and 0.05-0.1 parts of L-homoserine.

3. The soothing and antipruritic composition according to claim 1, characterized in that: In the soothing and antipruritic composition, the total mass percentage of Brazilian nectarine wood oil and L-homoserine is 65-75%.

4. The soothing and antipruritic composition according to claim 1, characterized in that: The mass percentage of hypericin in the Hypericum perforatum flower extract is 0.1-0.3%.

5. The soothing and antipruritic composition according to claim 4, characterized in that: The mass percentage of hypericin in the Hypericum perforatum flower extract is 0.14-0.2%.

6. The soothing and antipruritic composition according to claim 1, characterized in that: The preparation method of the Hypericum perforatum flower extract is as follows: crushing and sieving the Hypericum perforatum flower to obtain the Hypericum perforatum flower powder, mixing it with an ethanol solution and protecting it from light, rotating and shaking, centrifuging, collecting the supernatant, concentrating the supernatant and drying it to obtain the Hypericum perforatum flower extract.

7. The soothing and antipruritic composition according to claim 4, characterized in that: The volume fraction of the ethanol is 50-95%, and the weight ratio of the ethanol solution to the Hypericum perforatum flower powder is 8-12:

1.

8. The soothing and antipruritic composition according to claim 4, characterized in that: The rotation speed of the rotary oscillation is 100-150 r / min, and the rotation time is 48-72 h; and / or, the centrifugal speed is 4000-6000 r / min, the centrifugal temperature is 2-6° C., and the centrifugal time is 5-15 min.

9. Use of the soothing and antipruritic composition according to any one of claims 1 to 8 in the preparation of skin products.

10. A skin care product, characterized in that: The skin product comprises the soothing and antipruritic composition according to any one of claims 1 to 8.

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