Plant seed ground reinforcement material and method of ground reinforcement

Abstract

The application discloses a kind of plant seed reinforced ground material and ground reinforcing method, belong to ground reinforcing technical field.The above-mentioned plant seed reinforced ground material includes plant enzyme supernatant and reaction liquid;Wherein, the plant enzyme supernatant is prepared by the following method: seed dry powder is immersed in leaching solution, stirring forms suspension after being placed, and filtrate and supernatant I are obtained by filtration;The obtained residue is immersed in leaching solution again, stirred and placed, and supernatant II is collected by filtration, and supernatant I and supernatant II are uniformly mixed to be prepared;Reaction liquid is urea and calcium chloride mixed solution.In the application, raw materials used are less, and the raw materials involved do not contain acetone and other chemical solvents, and filter cloth and other consumables and by-products such as residue can be recycled, which realizes true renewable and pollution-free in raw material use;The preparation process is simple, and centrifugation and other plant enzyme extraction processes are not required, which can save a lot of manpower and financial resources, and is helpful to large-scale application of EICP technology.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of building, in particular to a plant seed reinforced foundation material and a foundation reinforcement method. BACKGROUND

[0002] Many plant seeds contain urease, and the urease in the seeds plays an important role in the metabolism of proteins during the germination process. In higher plants, the content of urease is mostly in the Cucurbitaceae and Leguminosae families. Studies have shown that the activity and content of urease gradually decrease with the germination of seeds. In recent years, it has been found that urease can be used to induce calcium carbonate precipitation for soil reinforcement (EICP). Almajed et al. reported in "Baseline investigation on enzyme-induced calcium carbonate precipitation" that urease extracted from soybeans can cement loose sand particles into sand columns with an unconfined strength greater than 1 MPa. Subsequently, researchers found that EICP can not only be used for soil reinforcement but also be applied to dust prevention and control, erosion control, and pollution control. EICP has the advantages of environmental friendliness, convenient construction, and low carbon emissions, and has important significance for the transformation of traditional cement-based construction methods with high energy consumption and high emissions. Therefore, it has become a research hotspot.

[0003] However, in the EICP reinforcement process, the urease consumption is large, the initial research uses extracted urease with high purity, and the monomer enzyme activity is high, but due to the complex extraction process, multiple centrifugation and addition of various chemicals are required, such as patent CN104480092A, which leads to high price, and the activity loss rate is high when used in environments with more impurities such as rock-soil. In order to reduce the cost of urease, researchers propose to extract crude enzyme from plant seeds for EICP. For example, Tirkolaei et al. proposed a three-step method for extracting crude enzyme from soybeans and watermelons in "Crude urease extract for biocementation". First, the shelled seeds are soaked in phosphate buffer and EDTA and broken with a homogenizer, filtered and high-speed centrifuged to obtain a crude extract. Then, the urease in the crude extract is fractionated with acetone at -20°C and high-speed centrifuged at low temperature to obtain a precipitate, and the precipitate is dissolved again in buffer and centrifuged to obtain the supernatant. Finally, crude enzyme is obtained by adding acetone again. Although this method greatly simplifies the urease extraction process, the use of a large amount of acetone in the extraction process may affect the health of construction personnel and produce a large amount of by-products. In addition, low-temperature centrifugation and other steps will also result in high cost for on-site engineering. He et al. proposed a method of crushing, soaking, salting out and centrifuging to collect the supernatant to prepare crude enzyme from soybeans in "Multiple-phase enzyme-induced carbonate precipitation (EICP) method for soil improvement", and the crude enzyme was used to reinforce Ottawa sand to obtain a sand column with a strength of more than 10 MPa. However, the urease solution obtained by using this crude extraction method contains a large amount of other impurity proteins, which is perishable at room temperature and has a very short shelf life (less than 12 h). Therefore, fresh urease solution needs to be extracted for each test to ensure the reinforcement effect. However, for actual engineering applications, on-site extraction of a large amount of crude enzyme not only requires additional equipment and cost, but also increases the construction process, which is high in cost and not conducive to on-site application. SUMMARY

[0004] To solve the above technical problems, the purpose of the present application is to provide a plant seed reinforced foundation material and a foundation reinforcement method to solve the problems of existing EICP technology, such as complex urease extraction steps, short enzyme solution storage time, complex construction, high cost, and not conducive to large-scale engineering application.

[0005] The technical solution of the present application to solve the above technical problems is as follows:

[0006] A plant seed reinforced foundation material, the plant seed reinforced foundation material comprising a plant enzyme clear liquid and a reaction liquid;

[0007] The plant enzyme clear solution is prepared by the following method:

[0008] (1) The seed dry powder is immersed in the leaching solution, stirred to form a suspension, and then left to stand. The filtrate I and the residue are obtained by filtration.

[0009] (2) The residue is immersed in the leaching solution again, stirred, and then left to stand. The filtrate II is collected by filtration. The filtrate I and the filtrate II are mixed uniformly to obtain the plant enzyme clear solution.

[0010] The reaction solution is a mixed solution of urea and calcium chloride.

[0011] Further, the seed includes at least one of watermelon seeds, soybeans, kidney beans, pumpkin seeds, and potatoes.

[0012] Further, the seed dry powder is prepared by the following method:

[0013] The seed is first dried and then ground into powder.

[0014] The grinding temperature is 40-80°C. The particle size of the seed dry powder is 200-400 mesh.

[0015] Further, the leaching solution is a sodium hypochlorite solution with a mass concentration of 0.05%-0.2%.

[0016] Further, in step (1), the mass ratio of the seed dry powder to the leaching solution is 0.5-2:3-7. The standing time is 2-5h.

[0017] Further, in step (2), the residue is repeatedly immersed in the leaching solution to collect the filtrate and mix it until the mass of the filtrate II is 8-12 times the mass of the seed dry powder. The mass ratio of the residue to the leaching solution is 0.5-2:1-3 each time. The standing time is 2-5h.

[0018] Further, the urease activity of the plant enzyme clear solution is ≥10U.

[0019] Further, the mass ratio of urea, calcium chloride, and water in the reaction solution is 0.5-2:5-10:17-34.

[0020] A method for reinforcing a foundation based on a plant seed reinforced foundation material, comprising the following steps:

[0021] After cleaning the surface of the foundation to be reinforced, the plant enzyme clear solution and the reaction solution are injected into the foundation to be reinforced for curing.

[0022] Further, the volume ratio of the plant enzyme clear solution to the reaction solution is 1:1-6. The injection method is at least one of alternating injection, alternating spraying, mixing and then injection, and mixing and then spraying. The total volume of the injected solution does not exceed 80% of the pore volume of the foundation to be reinforced.

[0023] Further, the curing time is 7-12h.

[0024] The present application has the following advantages:

[0025] (1) The raw materials used in the present application are less, and the raw materials do not contain acetone and other chemical solvents. The filter cloth and other consumables and by-products such as filter residue can be recycled, realizing true renewable and pollution-free use of raw materials.

[0026] (2) The preparation process of the present application is simple, and centrifugation and other plant enzyme extraction processes are not required. The preparation method is simple, which can save a lot of manpower and financial resources, and is helpful for large-scale application of EICP technology.

[0027] (3) The present application uses sodium hypochlorite solution as leaching solution, which can prevent the reproduction of microorganisms in the plant enzyme clear solution, thereby reducing the enzyme activity and greatly improving the storage time, which has commercial value. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 The flow chart for the preparation of the plant seed reinforced foundation material and the foundation reinforcement of the present application. DETAILED DESCRIPTION

[0029] The principles and characteristics of the present application are described below in conjunction with the drawings, and the examples are only used to explain the present application and are not used to limit the scope of the present application. If the specific conditions are not specified in the examples, the conventional conditions or the conditions recommended by the manufacturer are used. If the reagents or instruments used are not specified by the manufacturer, they are all conventional products that can be purchased on the market.

[0030] Example 1:

[0031] A preparation method of a plant seed reinforced foundation material, comprising the following steps:

[0032] (1) Select soybeans as plant seeds, first dry the plant seeds in the sun and then dry them in an oven until the surface is dry, then grind the dried plant seeds into powder by a flour mill, control the temperature of the dry powder during the grinding process to be 60℃, and sieve to obtain a seed dry powder of 300 mesh.

[0033] (2) First, add one part (mass fraction) of seed dry powder to five parts of leaching solution (mass fraction) (0.1% sodium hypochlorite solution), stir to form a suspension, then stand for 4h, after the suspension is layered, filter with a filter screen, and use a multi-stage filter screen arranged from top to bottom to filter and separate to obtain filter residue and clear liquid I;

[0034] (3) one part (mass fraction) of the filter residue is added into two parts (mass fraction) of the leaching liquid (0.05% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and the filter residue is repeatedly treated with the leaching liquid, and the clear liquid obtained after each treatment is collected until the total mass of the clear liquid is ten times the mass of the seed dry powder. The clear liquids are mixed to obtain clear liquid II, and finally, the clear liquid I and the clear liquid II are mixed to obtain the plant enzyme clear liquid.

[0035] (4) one part (mass fraction) of industrial urea, seven parts (mass fraction) of industrial calcium chloride are dissolved in twenty parts (mass fraction) of water, and after cooling, a reaction liquid is obtained.

[0036] A method for reinforcing a foundation based on a plant seed reinforced foundation material, comprising the following steps:

[0037] (a) cleaning the surface of the foundation to be reinforced to remove weeds, tree roots and other debris;

[0038] (b) mixing the plant enzyme clear liquid and the reaction liquid at a volume ratio of 1:1, and then injecting them into the cleaned foundation. The total volume of the injection does not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, the foundation is cured for 10 hours;

[0039] (c) repeating step (b) until the strength of the foundation to be reinforced reaches the required value.

[0040] Example 2:

[0041] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0042] (1) selecting potatoes as plant seeds, first drying the plant seeds, and then grinding the dried plant seeds into powder through a flour mill. The temperature of the dry powder during the grinding process is controlled at 60°C, and the seed dry powder with a particle size of 300 mesh is obtained by sieving.

[0043] (2) one part (mass fraction) of the seed dry powder is first added into five parts (mass fraction) of the leaching liquid (0.05% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and the filter residue and the clear liquid I are obtained by filtering and separating with a multi-stage filter screen arranged from top to bottom.

[0044] (3) one part (mass fraction) of the filter residue is added into two parts (mass fraction) of the leaching solution (0.1% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and the filter residue is repeatedly treated with the leaching solution, and the clear liquid obtained after each treatment is collected until the total mass of the clear liquid is ten times the mass of the seed dry powder. The clear liquids are mixed to obtain clear liquid II. Finally, the clear liquid I and the clear liquid II are mixed to obtain the plant enzyme clear liquid.

[0045] (4) one part (mass fraction) of industrial urea, seven parts (mass fraction) of industrial calcium chloride are dissolved in twenty parts (mass fraction) of water, and after cooling, a reaction solution is obtained.

[0046] A method for reinforcing a foundation based on a plant seed reinforced foundation material, comprising the following steps:

[0047] (a) cleaning the surface of the foundation to be reinforced to remove weeds, tree roots and other debris;

[0048] (b) injecting the plant enzyme clear liquid and the reaction solution alternately (first injecting the plant enzyme clear liquid, then injecting the reaction solution, and alternating for 5 cycles) into the cleaned foundation at a volume ratio of 1:1. The total volume of injection does not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, it is cured for 10 hours;

[0049] (c) repeating step (b) until the strength of the foundation to be reinforced reaches the required value.

[0050] Example 3:

[0051] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0052] (1) selecting watermelon seeds as plant seeds, first drying the plant seeds, then drying the plant seeds to remove surface moisture, then grinding the dried plant seeds into powder by a flour mill, controlling the temperature of the dry powder during the grinding process to be 60°C, and sieving to obtain 300 mesh seed dry powder.

[0053] (2) one part (mass fraction) of the seed dry powder is first added into five parts (mass fraction) of the leaching solution (0.2% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, it is filtered with a filter screen, and the filter residue and the clear liquid I are obtained by filtering and separating with a multi-stage filter screen arranged from top to bottom.

[0054] (3) one part (mass fraction) of the filter residue is added into two parts (mass fraction) of the leaching solution (0.1% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, the filter residue is treated with the leaching solution repeatedly for several times by filtering with a filter screen arranged from top to bottom, and the clear liquid obtained after each treatment is collected until the total mass of the clear liquid is ten times the mass of the seed dry powder. The clear liquids are mixed to obtain clear liquid II. Finally, the clear liquid I and the clear liquid II are mixed to obtain the plant enzyme clear liquid.

[0055] (4) one part (mass fraction) of industrial urea and seven parts (mass fraction) of industrial calcium chloride are dissolved in twenty parts (mass fraction) of water, and after cooling, a reaction solution is obtained.

[0056] A method for reinforcing a foundation based on a plant seed reinforced foundation material, comprising the following steps:

[0057] (a) cleaning the surface of the foundation to be reinforced to remove weeds, tree roots and other sundries;

[0058] (b) mixing the plant enzyme clear liquid and the reaction solution at a volume ratio of 1:1, and then injecting them into the cleaned foundation. The total volume of the injection does not exceed 80% of the pore volume of the foundation to be reinforced. After the injection is completed, the foundation is cured for 10 hours;

[0059] (c) repeating step (b) until the strength of the foundation to be reinforced reaches the required value.

[0060] Example 4:

[0061] A method for preparing a plant seed reinforced foundation material, comprising the following steps:

[0062] (1) Selecting Canavalia as the plant seed, first drying the plant seed, and then grinding the dried plant seed into powder by a flour mill. The temperature of the dry powder during the grinding process is controlled at 60°C, and the seed dry powder with a particle size of 300 mesh is obtained by sieving.

[0063] (2) one part (mass fraction) of the seed dry powder is added into five parts (mass fraction) of the leaching solution (0.05% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is stratified, the filter residue and the clear liquid I are separated by pressure filtration.

[0064] (3) one (mass fraction) of the filter residue is added into two (mass fraction) of the leaching liquid (0.1% sodium hypochlorite solution), stirred to form a suspension, and then left to stand for 4 hours. After the suspension is layered, the filter residue is treated with the leaching liquid repeatedly for several times, and the clear liquid obtained after each treatment is collected until the total mass of the clear liquid is ten times the mass of the seed dry powder. The clear liquids are mixed to obtain clear liquid II. Finally, the clear liquid I and the clear liquid II are mixed to obtain the plant enzyme clear liquid.

[0065] (4) one (mass fraction) of industrial urea, seven (mass fraction) of industrial calcium chloride are dissolved in twenty (mass fraction) of water, and after cooling, a reaction liquid is obtained.

[0066] A method for reinforcing a foundation based on a plant seed reinforced foundation material, comprising the following steps:

[0067] (a) cleaning the surface of the foundation to be reinforced to remove weeds, tree roots and other sundries;

[0068] (b) mixing the plant enzyme clear liquid and the reaction liquid at a volume ratio of 1:1, and then injecting the mixture into the cleaned foundation. The total volume of the injection is not more than 80% of the pore volume of the foundation to be reinforced. After the injection is completed, the foundation is cured for 10 hours;

[0069] (c) repeating step (b) until the strength of the foundation to be reinforced reaches the required value.

[0070] The above description is only the preferred embodiment of the present application, and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A plant seed-based foundation reinforcement material, characterized in that, Plant seed-based foundation reinforcement materials include plant enzyme solutions and reaction solutions; The plant enzyme solution is prepared by the following method: (1) Soak the dried seed powder in the filtrate, stir to form a suspension, let it stand, filter to obtain the residue and clear liquid I; (2) The obtained filter residue is immersed in the filtrate again, stirred and allowed to stand, filtered and the clear liquid II is collected. Clear liquid I and clear liquid II are combined and mixed evenly to obtain the product. The reaction solution is a mixture of urea, calcium chloride and water; The seeds include at least one of watermelon seeds, soybeans, sword beans, pumpkin seeds, and potatoes; The seed powder was prepared by the following method: First, dry the seeds in the sun until there is no moisture on the surface, and then grind them into powder; The grinding process temperature is 40-80℃; the particle size of the dry seed powder is 200-400 mesh. The leaching solution is a sodium hypochlorite solution with a mass concentration of 0.05%-0.2%.

2. The plant seed-based foundation reinforcement material according to claim 1, characterized in that, In step (1), the mass ratio of seed powder to leaching liquid is 0.5-2:3-7; the standing time is 2-5 h.

3. The plant seed-based foundation reinforcement material according to claim 1, characterized in that, In step (2), the filter residue is repeatedly immersed in the leaching liquid to collect the clear liquid and combine them until the mass of the clear liquid II is 8-12 times the mass of the seed dry powder. The mass ratio of the filter residue to the leaching liquid is 0.5-2:1-3 each time it is immersed; the standing time is 2-5 h.

4. The plant seed-based foundation reinforcement material according to claim 1, characterized in that, The mass ratio of urea, calcium chloride and water in the reaction solution is 0.5-2:5-10:17-34.

5. A method for foundation reinforcement based on the plant seed-based foundation reinforcement material according to any one of claims 1-4, characterized in that, Includes the following steps: After cleaning the surface of the foundation to be reinforced, inject the plant enzyme solution and reaction solution into the foundation for curing.

6. The foundation reinforcement method according to claim 5, characterized in that, The volume ratio of the plant enzyme solution to the reaction solution is 1:1-6; the injection method is at least one of alternating injection, alternating spraying, injection after mixing, and spraying after mixing; the total volume of the injected solution does not exceed 80% of the pore volume of the foundation to be reinforced.

7. The foundation reinforcement method according to claim 5, characterized in that, The maintenance time is 7-12 hours.

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