Micro-fluidic chip and research method for oxygen supply mechanism of vascular smooth muscle cells

By designing a microfluidic chip to simulate the environment of vascular endothelial cells and vascular smooth muscle cells, the problem that the existing technology cannot effectively explain the oxygen supply mechanism of vascular smooth muscle cells is solved, and a more accurate in vitro model is achieved, providing an effective means for the study of the oxygen supply mechanism of vascular smooth muscle cells.

CN119752621APending Publication Date: 2025-04-04CHINESE PEOPLES LIBERATION ARMY ARMY SPECIAL MEDICAL CENTER
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Patent Information

Application Number
CN202411950163.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-27
Publication Date
2025-04-04

AI Technical Summary

Technical Problem

The prior art cannot effectively explain the oxygen supply mechanism of vascular smooth muscle cells, mainly because the solubility and free diffusion rate of O2 in aqueous solution are low, and the free diffusion oxygen supply method leads to hypoxia or oxygen poisoning of some vascular smooth muscle cells.

Method used

A microfluidic chip is designed to simulate the environment of vascular endothelial cells and vascular smooth muscle cells in the body, simulate the change pattern of the vascular lumen and vascular smooth muscle cell layers through the culture channel with gradually narrowing width, establish an in vitro model of the muscle endothelial junction system, and study the oxygen supply mechanism of vascular smooth muscle cells.

Benefits of technology

An in vitro model is provided that can more accurately simulate the oxygen supply mechanism of vascular smooth muscle cells, filling the gap in the prior art that cannot effectively study such cellular oxygen supply mechanisms.

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Abstract

The invention discloses a micro-fluidic chip and a research method of a vascular smooth muscle cell oxygen supply mechanism based on the chip, the micro-fluidic chip is formed by bonding a cover glass layer and a main body layer, and the main body layer is provided with a vascular endothelial cell and vascular smooth muscle cell culture channel with gradually narrowed width. The two channels are communicated through a plurality of micro-channels of which the distance is gradually shortened; vascular endothelial cells and vascular smooth muscle cells are inoculated into the cell culture channel to establish a muscle endothelial connection system model, a fluorescent probe is loaded into the vascular smooth muscle cells, and the influence of compounds or genes on oxygen supply of the vascular smooth muscle cells is analyzed through a fluorescence microscope. According to the micro-fluidic chip and the research method of the vascular smooth muscle cell oxygen supply mechanism based on the micro-fluidic chip, the blank in the field is filled, and related research can be carried out in vitro in advance.
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