A method and apparatus for detecting gut flora

Abstract

The application discloses a kind of intestinal flora detection method and device, it is related to intestinal flora detection technical field, including support frame, the side of support frame is respectively equipped with microscope and support plate, the upper portion of support frame is equipped with collection box, the inboard of support plate is slidably provided with moving plate, moving plate is provided with clamping assembly, clamping assembly includes the worm and rotating rod rotatably connected on moving plate, and a plurality of clamping plates movably connected on rotating rod, worm rotation drives protective shell to rotate, protective shell rotates can drive a plurality of clamping plates to rotate to the direction close to moving plate and hold object slide, moving plate passes through the detection of itself movement and makes a plurality of clamping plates respectively through microscope, collection assembly is provided on support plate, in the application, manual operation is reduced by automatic process, so that detection process is more efficient, ensure that the detection process of each sample is standardized, improve the consistency of result.

Description

Technical Field

[0001] This invention relates to the field of intestinal flora detection technology, and in particular to a method and apparatus for intestinal flora detection. Background Technology

[0002] The gut microbiota refers to the community of microorganisms living in the human gut, including bacteria, viruses, fungi, and archaea, with bacteria making up the vast majority. These microorganisms form a complex symbiotic relationship with the human body and play a vital role in maintaining human health. The state of the gut microbiota is closely related to human health. By monitoring the gut microbiota, we can understand the body's health status and detect potential disease risks in a timely manner. Microscopy is one of the important tools for studying the gut microbiota, allowing for direct observation of its morphology, structure, and distribution.

[0003] A search revealed that Chinese patent number CN118778239A discloses an optical microscope with a stable anti-deviation structure. Compared with the prior art, this invention patent uses a second anti-deviation plate to stabilize and limit the side wall of the thin film, while the first anti-deviation plate stabilizes and limits the top wall of the thin film under the action of a support spring. This allows the optical microscope to achieve anti-deviation operation of the thin film through the first and second anti-deviation plates, thereby preventing interference with the operator's observation of the object. The operation process is also simple and quick.

[0004] However, in actual use, when multiple fecal samples need to be tested, the staff needs to repeatedly fix, disassemble, and collect the slide samples, which is cumbersome and results in low testing efficiency. Therefore, a method and device for detecting intestinal flora is proposed. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies, which involve repeated installation, disassembly, and collection of slides, resulting in cumbersome processes and low detection efficiency. This invention proposes a method and apparatus for detecting intestinal flora.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] An intestinal flora detection device includes a support frame. A microscope and a support plate are respectively mounted on the sides of the support frame. A collection box is mounted on the upper part of the support frame. A movable plate is slidably disposed on the inner side of the support plate. Different glass slides can be monitored by sliding the movable plate. A clamping assembly is disposed on the movable plate. The clamping assembly includes a worm gear and a rotating rod rotatably connected on the movable plate, and multiple clamping plates movably connected to the rotating rod. When the worm gear rotates, it drives the rotating rod to rotate. When the rotating rod rotates, it drives the multiple clamping plates to rotate towards the movable plate to clamp the glass slides. The movable plate, through its own... The moving plate allows multiple clamping plates to be inspected separately under a microscope. The automated process reduces manual operation and makes the inspection process more efficient. The support plate is equipped with a collection assembly, which includes a squeezing plate mounted on the support plate and a push rod movably connected to the support plate. The moving plate has multiple collection slots inside. When the moving plate moves, the squeezing plate can squeeze the clamping plate. After being squeezed, the clamping plate generates an upward force and separates from the glass slide. When the push rod moves, it pushes the glass slide through the collection slots and falls into the collection box, reducing manual handling of the glass slide after inspection and improving work efficiency.

[0008] The above technical solution further includes:

[0009] The support plate has a first opening groove inside, and the movable plate is slidably disposed inside the first opening groove. The size of the opening of the first opening groove is adapted to the size of the movable plate, so that the movable plate can slide stably along the first opening groove.

[0010] The upper part of the movable plate has a square groove, and the worm gear is rotatably connected to the inner side of the square groove. A protective shell is installed on the upper part of the movable plate, and the rotating rod is rotatably connected to the inner side of the protective shell. A worm wheel is installed on the outer side of the rotating rod, and the worm wheel meshes with the worm gear. When the worm gear is rotated, the worm wheel can be driven to rotate.

[0011] Multiple square blocks are installed on the outer side of the rotating rod. The square blocks are movably connected to the clamping plate. A rubber plate is installed on the side of the clamping plate near the moving plate. The rubber plate can increase the friction with the glass slide. The elasticity of the rubber plate can be used to fix glass slides of different thicknesses.

[0012] The clamping plate has a second opening groove inside, and the squeezing plate is located on the movement trajectory of the clamping plate and the edge of the second opening groove. The squeezing plate can squeeze the clamping plate to separate it from the glass slide.

[0013] The square block has a first sliding groove on the side near the clamping plate. A first sliding block is slidably disposed inside the first sliding groove. A first spring is installed on the upper part of the first sliding block. The end of the first spring away from the first sliding block is fixedly connected to the inner wall of the first sliding groove. The side of the first sliding block away from the rotating rod is fixedly connected to the clamping plate. When the clamping plate moves, it can move along the first sliding groove through the first sliding block.

[0014] A rack is installed at the bottom of the movable plate, and a rotating handle is rotatably connected to the bottom of the support plate. A gear is installed at the end of the rotating handle near the rack, and the gear meshes with the rack. Rotating the rotating handle can drive the movable plate to move through the gear and rack.

[0015] The upper part of the support plate is provided with a second sliding groove, and a second sliding block is slidably arranged inside the second sliding groove. A second spring is installed on the side of the second sliding block. The end of the second spring away from the second sliding block is fixedly connected to the inner wall of the second sliding groove. A movable block is movably connected to the upper part of the second sliding block. The side of the movable block near the clamping plate is fixedly connected to the push rod, so that the push rod moves horizontally to push the glass slide.

[0016] The upper part of the second sliding block is respectively equipped with a square rod and a third spring. The square rod is slidably connected to the movable block, and the end of the third spring away from the second sliding block is fixedly connected to the movable block. Pressing the movable block can make the push rod contact the moving plate.

[0017] A method for using an intestinal flora detection device includes the following steps:

[0018] Step 1: Rotate the worm gear to drive the clamping plate on the rotating rod to rotate away from the moving plate, place multiple glass slides at the clamping plate respectively, and reverse the worm gear to fix multiple glass slides at the same time through multiple clamping plates;

[0019] Step 2: Move the moving plate, which will move the glass slides at different clamping plates to the bottom of the microscope for testing.

[0020] Step 3: After the test is completed, the slide is moved to the squeezing plate by the moving plate. The squeezing plate squeezes the clamping plate, separating the clamping plate from the slide. The push rod pushes the slide, causing it to fall through the collection groove into the collection box for automatic collection.

[0021] The present invention has the following beneficial effects:

[0022] 1. In this invention, multiple glass slides are quickly and simultaneously clamped by a clamping component, and then the glass slides are moved to the bottom of the microscope for detection by the movement of the moving plate. The automated process reduces manual operation, makes the detection process more efficient, ensures that the detection process of each sample is standardized, and improves the consistency of the results.

[0023] 2. In this invention, the collection component can quickly collect the slides after the test is completed, reducing the manual handling of the slides after the test, improving work efficiency, and also helping to prevent sample leakage and contamination. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall side structure of the intestinal flora detection method and device proposed in this invention;

[0025] Figure 2 This is a schematic diagram of the overall side structure of the present invention;

[0026] Figure 3 This is a schematic diagram of the connection structure between the square block and the clamping plate in this invention;

[0027] Figure 4 This is a schematic diagram of the extrusion plate structure in this invention;

[0028] Figure 5 for Figure 1 Enlarged schematic diagram of the structure at point A in the middle;

[0029] Figure 6 for Figure 1 Enlarged schematic diagram of the structure at point B;

[0030] Figure 7 for Figure 2 Enlarged schematic diagram of the structure at point C;

[0031] Figure 8 for Figure 2 Enlarged schematic diagram of the structure at point D;

[0032] Figure 9 for Figure 3 Enlarged schematic diagram of the structure at point E in the middle.

[0033] In the diagram: 1. Support frame; 2. Microscope; 3. Support plate; 4. First opening slot; 5. Moving plate; 6. Rack; 7. Rotating handle; 8. Gear; 9. Square slot; 10. Worm gear; 11. Protective shell; 12. Rotating rod; 13. Worm wheel; 14. Square block; 15. Clamping plate; 16. Second opening slot; 17. Rubber plate; 18. First sliding slot; 19. First sliding block; 20. First spring; 21. Squeezing plate; 22. Collection slot; 23. Second sliding slot; 24. Second sliding block; 25. Second spring; 26. Square rod; 27. Third spring; 28. Movable block; 29. ​​Push rod; 30. Collection box. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] Example 1

[0036] like Figures 1-9 As shown, the present invention proposes an intestinal flora detection device, including a support frame 1. A microscope 2 and a support plate 3 are respectively mounted on the side of the support frame 1. A collection box 30 is mounted on the upper part of the support frame 1. A movable plate 5 is slidably disposed on the inner side of the support plate 3. Different glass slides can be monitored by sliding the movable plate 5. A clamping assembly is disposed on the movable plate 5. The clamping assembly includes a worm gear 10 and a rotating rod 12 rotatably connected to the movable plate 5, and multiple clamping plates 15 movably connected to the rotating rod 12. When the worm gear 10 rotates, it drives the rotating rod 12 to rotate. When the rotating rod 12 rotates, it can drive the multiple clamping plates 15 to rotate towards the movable plate 5 to clamp the glass slides. The moving plate 5 moves to allow multiple clamping plates 15 to be inspected by the microscope 2. This automated process reduces manual operation and makes the inspection process more efficient. The support plate 3 is equipped with a collection component, which includes a squeezing plate 21 mounted on the support plate 3 and a push rod 29 movably connected to the support plate 3. The moving plate 5 has multiple collection slots 22 inside. When the moving plate 5 moves, it can squeeze the clamping plates 15 through the squeezing plate 21. After being squeezed, the clamping plates 15 generate an upward force and separate from the glass slides. When the push rod 29 moves, it pushes the glass slides through the collection slots 22 and into the collection box 30. This reduces the manual handling of the glass slides after inspection and improves work efficiency.

[0037] The support plate 3 has a first opening groove 4 inside, and the movable plate 5 is slidably disposed inside the first opening groove 4. The size of the opening of the first opening groove 4 is adapted to the size of the movable plate 5, so that the movable plate 5 can slide stably along the first opening groove 4.

[0038] The upper part of the movable plate 5 is provided with a square groove 9. The worm gear 10 is rotatably connected to the inner side of the square groove 9. A protective shell 11 is installed on the upper part of the movable plate 5. The rotating rod 12 is rotatably connected to the inner side of the protective shell 11. A worm wheel 13 is installed on the outer side of the rotating rod 12, and the worm wheel 13 meshes with the worm gear 10. When the worm gear 10 is rotated, the worm wheel 13 can be driven to rotate through the worm wheel 13.

[0039] Multiple square blocks 14 are installed on the outer side of the rotating rod 12. The square blocks 14 are movably connected to the clamping plate 15. A rubber plate 17 is installed on the side of the clamping plate 15 near the moving plate 5. The rubber plate 17 can increase the friction with the glass slide. The elasticity of the rubber plate 17 can be used to fix glass slides of different thicknesses.

[0040] A rack 6 is installed at the bottom of the movable plate 5, and a rotating handle 7 is rotatably connected to the bottom of the support plate 3. A gear 8 is installed at the end of the rotating handle 7 near the rack 6. The gear 8 meshes with the rack 6. Rotating the rotating handle 7 can drive the movable plate 5 to move through the gear 8 and the rack 6.

[0041] In this embodiment, when intestinal flora detection is required, the sample can first be smeared on a glass slide. At this time, the worm gear 10 can be rotated. Since the worm gear 10 meshes with the worm wheel 13, the worm gear 10 can drive the rotating rod 12 to rotate through the worm wheel 13. When the rotating rod 12 rotates, it can drive the clamping plate 15 to rotate away from the moving plate 5. At this time, different glass slides can be placed at the corresponding positions of multiple clamping plates 15. Then, the worm gear 10 is reversed, and the rotating rod 12 is reversed through the worm gear 10, thereby causing the clamping plate 15 to reverse and clamp the glass slide. The clamping plate 15 contacts the glass slide through the rubber plate 17, which can increase the friction. The elasticity of the rubber plate 17 can be used to fix glass slides of different thicknesses, so that multiple glass slides can be quickly fixed.

[0042] Then, rotating the handle 7 drives the gear 8 to rotate. Since the gear 8 meshes with the rack 6, rotating the handle 7 allows the moving plate 5 to move along the first opening groove 4 through the gear 8 and rack 6 until the slide is moved under the microscope 2. Then, the detection work is carried out. Based on the observed total number of bacteria, morphological characteristics, the ratio of Gram-positive to Gram-negative bacteria, the ratio of bacilli to cocci, and whether there is an increase in special morphological bacteria, a comprehensive judgment is made on whether there is a bacterial imbalance. After the sample on the current slide is detected, rotating the handle 7 again drives the moving plate 5 to move until the next slide is moved under the microscope 2 for detection. This quickly fixes multiple slides, and then the moving plate 5 moves different slides under the microscope 2 for detection. The automated process reduces manual operation, makes the detection process more efficient, ensures that the detection process of each sample is standardized, and improves the consistency of the results.

[0043] Example 2

[0044] like Figures 1-9 As shown, based on Embodiment 1, the clamping plate 15 has a second opening groove 16 inside, and the pressing plate 21 is located on the movement trajectory of the clamping plate 15 and the edge of the second opening groove 16. The pressing plate 21 can press the clamping plate 15 to separate it from the glass slide.

[0045] The square block 14 has a first sliding groove 18 on the side near the clamping plate 15. A first sliding block 19 is slidably disposed inside the first sliding groove 18. A first spring 20 is installed on the upper part of the first sliding block 19. The end of the first spring 20 away from the first sliding block 19 is fixedly connected to the inner wall of the first sliding groove 18. The side of the first sliding block 19 away from the rotating rod 12 is fixedly connected to the clamping plate 15. When the clamping plate 15 moves, it can move along the first sliding groove 18 through the first sliding block 19.

[0046] The upper part of the support plate 3 is provided with a second sliding groove 23. A second sliding block 24 is slidably disposed inside the second sliding groove 23. A second spring 25 is installed on the side of the second sliding block 24. The end of the second spring 25 away from the second sliding block 24 is fixedly connected to the inner wall of the second sliding groove 23. A movable block 28 is movably connected to the upper part of the second sliding block 24. The side of the movable block 28 near the clamping plate 15 is fixedly connected to the push rod 29, so that the push rod 29 moves horizontally to push the glass slide.

[0047] The upper part of the second sliding block 24 is respectively equipped with a square rod 26 and a third spring 27. The square rod 26 is slidably connected to the movable block 28. The end of the third spring 27 away from the second sliding block 24 is fixedly connected to the movable block 28. Pressing the movable block 28 can make the push rod 29 contact the moving plate 5.

[0048] In this embodiment, after the sample testing of the current slide is completed, when the moving plate 5 is moved to test the next slide, the clamping plate 15 fixed to the previous slide moves to the squeezing plate 21. Then, the squeezing plate 21 squeezes the inclined surface at the connection between the clamping plate 15 and the second opening groove 16 until the squeezing plate 21 moves into the interior of the second opening groove 16. At this time, the clamping plate 15 moves along the first sliding groove 18 through the squeezing force via the first sliding block 19. At the same time, the first spring 20 contracts, and the clamping plate 15 drives the rubber plate 17 to move upward, and then automatically releases the fixation of the slide. At this time, the movable block 28 can be pressed to move the movable block 28 down into the second sliding groove 28. Within step 3, the third spring 27 contracts, and when the movable block 28 moves down, it drives the push rod 29 to move. At this time, the push rod 29 contacts the movable plate 5, and then pushes the second sliding block 24 through the movable block 28, causing the second sliding block 24 to move along the second sliding groove 23. At this time, the second spring 25 contracts, and the second sliding block 24 moves to a position where it can drive the push rod 29 through the movable block 28. The push rod 29 moves to a position where it can push the glass slide, pushing the glass slide to the collection groove 22, so that the glass slide falls into the collection box 30 through the collection groove 22. In this way, the glass slide can be quickly collected after the test, reducing the manual handling of the glass slide after the test, improving work efficiency, and also helping to prevent sample leakage and contamination.

[0049] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An intestinal flora detection device, comprising a support frame (1), characterized in that, The support frame (1) is equipped with a microscope (2) and a support plate (3) on its side, and a collection box (30) is installed on the upper part of the support frame (1). A movable plate (5) is slidably disposed on the inner side of the support plate (3). A clamping assembly is disposed on the movable plate (5). The clamping assembly includes a worm gear (10) and a rotating rod (12) rotatably connected on the movable plate (5), and multiple clamping plates (15) movably connected on the rotating rod (12). When the worm gear (10) rotates, it drives the rotating rod (12) to rotate. When the rotating rod (12) rotates, it can drive the multiple clamping plates (15) to rotate towards the movable plate (5) to clamp the glass slide. The moving plate (5) moves itself to allow multiple clamping plates (15) to be inspected by the microscope (2) respectively. The support plate (3) is provided with a collection component, which includes a pressing plate (21) installed on the support plate (3) and a push rod (29) movably connected to the support plate (3). The moving plate (5) has multiple collection slots (22) inside. When the moving plate (5) moves, it can press the clamping plate (15) through the pressing plate (21). After being pressed, the clamping plate (15) generates an upward force and separates from the glass slide. When the push rod (29) moves, it pushes the glass slide through the collection slots (22) and drops it into the collection box (30). The upper part of the support plate (3) is provided with a second sliding groove (23), and a second sliding block (24) is slidably arranged inside the second sliding groove (23). A second spring (25) is installed on the side of the second sliding block (24). The end of the second spring (25) away from the second sliding block (24) is fixedly connected to the inner wall of the second sliding groove (23). A movable block (28) is movably connected to the upper part of the second sliding block (24). The side of the movable block (28) near the clamping plate (15) is fixedly connected to the push rod (29). The upper part of the second sliding block (24) is respectively equipped with a square rod (26) and a third spring (27). The square rod (26) is slidably connected to the movable block (28), and the end of the third spring (27) away from the second sliding block (24) is fixedly connected to the movable block (28).

2. The intestinal flora detection device according to claim 1, characterized in that, The support plate (3) has a first opening groove (4) inside, and the movable plate (5) is slidably disposed inside the first opening groove (4), and the size of the opening of the first opening groove (4) is adapted to the size of the movable plate (5).

3. The intestinal flora detection device according to claim 2, characterized in that, The upper part of the movable plate (5) is provided with a square groove (9), the worm (10) is rotatably connected to the inner side of the square groove (9), the upper part of the movable plate (5) is provided with a protective shell (11), the rotating rod (12) is rotatably connected to the inner side of the protective shell (11), the outer side of the rotating rod (12) is provided with a worm wheel (13), and the worm wheel (13) meshes with the worm (10).

4. The intestinal flora detection device according to claim 1, characterized in that, Multiple square blocks (14) are installed on the outside of the rotating rod (12). The square blocks (14) are movably connected to the clamping plate (15). A rubber plate (17) is installed on the side of the clamping plate (15) near the moving plate (5).

5. The intestinal flora detection device according to claim 4, characterized in that, The clamping plate (15) has a second opening groove (16) inside, and the extrusion plate (21) is located on the movement trajectory of the clamping plate (15) and the edge of the second opening groove (16).

6. The intestinal flora detection device according to claim 4, characterized in that, The square block (14) has a first sliding groove (18) on the side near the clamping plate (15). A first sliding block (19) is slidably arranged inside the first sliding groove (18). A first spring (20) is installed on the upper part of the first sliding block (19). The end of the first spring (20) away from the first sliding block (19) is fixedly connected to the inner wall of the first sliding groove (18). The side of the first sliding block (19) away from the rotating rod (12) is fixedly connected to the clamping plate (15).

7. The intestinal flora detection device according to claim 1, characterized in that, A rack (6) is installed at the bottom of the movable plate (5), and a rotating handle (7) is rotatably connected to the bottom of the support plate (3). A gear (8) is installed at the end of the rotating handle (7) near the rack (6), and the gear (8) meshes with the rack (6).

8. The method used in the intestinal flora detection device according to claims 1-7, characterized in that, Includes the following steps: Step 1: Rotate the worm gear (10) to drive the clamping plate (15) on the rotating rod (12) to rotate away from the moving plate (5), place multiple glass slides at the clamping plate (15) respectively, and reverse the worm gear (10) to fix multiple glass slides at the same time through multiple clamping plates (15); Step 2: Move the moving plate (5). The moving plate (5) will move the glass slides at different clamping plates (15) to the bottom of the microscope (2) for testing. Step 3: After the test is completed, the slide is moved to the squeezing plate (21) by the moving plate (5). The squeezing plate (21) squeezes the clamping plate (15), and the clamping plate (15) separates from the slide. The pushing rod (29) pushes the slide, so that the slide falls through the collection groove (22) into the collection box (30) for automatic collection.

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