Tobacco raw material treatment method
By using enzyme preparation treatment methods in tobacco raw materials, the macromolecular substances and higher fatty acids in tobacco raw materials are decomposed, and the sour taste, spicy taste and irritation problems in the cigarettes that are heated and not burned are solved, and the sensory quality is improved.
Patent Information
- Application Number
- CN202311427828.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-30
- Publication Date
- 2025-05-02
AI Technical Summary
Current heating-free cigarettes will produce a strong sour and spicy taste when heated, and are highly irritating. The treatment method of tobacco raw materials still needs to be improved.
A treatment method for tobacco raw materials is adopted, including dissolving the enzyme preparation powder in water to form an enzyme solution, adjusting the pH value of the enzyme solution and performing activation treatment, spraying it into the tobacco raw material, placing it in a constant temperature and humidity environment, inactivating it and balancing it in an equilibrium environment, thereby decomposing the macromolecular substances in the tobacco raw material and reducing the sour taste, spicy taste and irritation.
By decomposing the macromolecular substances and higher fatty acids in tobacco raw materials, it reduces the sourness, spicyness and irritation of the cigarette when smoking with heating and non-combustible cigarettes, and improves the sensory quality when smoking with heating and non-combustible cigarettes.
Smart Images
Figure CN119908509A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of tobacco technology, and in particular, to a method for processing tobacco raw materials. Background Art
[0002] Heat-not-burn cigarettes are a smoking method that heats tobacco substances through electronic devices instead of burning them. When smoking with electronic heating devices, consumers can not only feel the aroma of tobacco, but also heat the tobacco core at a lower temperature, which can significantly reduce the release of tar and other undesirable substances in mainstream smoke. This smoking method is becoming more and more popular at home and abroad, and heat-not-burn cigarettes have become the mainstream direction of development in the tobacco industry.
[0003] However, current heat-not-burn cigarettes produce strong sour and spicy flavors when heated, which are highly irritating. Therefore, the current method for processing tobacco raw materials still needs to be improved. Summary of the invention
[0004] The present application aims to alleviate or solve at least one of the above-mentioned problems to at least some extent.
[0005] In one aspect of the present application, the present application proposes a method for processing tobacco raw materials. In some embodiments of the present application, the method for processing tobacco raw materials includes the following steps: (1) dissolving enzyme preparation powder in water to form an enzyme solution, adjusting the pH value of the enzyme solution, and then activating the enzyme solution; (2) spraying the activated enzyme solution onto the tobacco raw material; (3) placing the tobacco raw material sprayed with the enzyme solution in a constant temperature and humidity environment for treatment; (4) inactivating the tobacco raw material treated in step (3); (5) placing the inactivated tobacco raw material in a balanced environment for balanced treatment. Thus, after the activation treatment, the enzyme in the enzyme solution can decompose the macromolecular substances in the tobacco raw material into small molecules, thereby reducing the sourness, spiciness and irritation of the heat-not-burn cigarette when smoking, and improving the sensory quality of the heat-not-burn cigarette when smoking.
[0006] In some embodiments of the present application, in step (1), the enzyme preparation includes at least one of protease, amylase, saccharifying enzyme and cellulase. The enzyme preparation can decompose macromolecular substances such as protein, starch, cellulose and / or higher fatty acids in tobacco raw materials, thereby improving the sensory smoking quality of heat-not-burn cigarettes.
[0007] In some embodiments of the present application, in step (1), the mass content of the enzyme preparation in the enzyme solution is 0.5%-5%.
[0008] In some embodiments of the present application, in step (1), the pH value of the enzyme solution is adjusted to 6-8; and / or, in step (1), at least one of citric acid and / or malic acid is added to the enzyme solution to adjust the pH value of the enzyme solution.
[0009] In some embodiments of the present application, in step (1), the temperature of the activation treatment is 30°C-50°C, and / or the time of the activation treatment is 15min-2h. The temperature of the activation treatment and / or the time of the activation treatment within the above range can effectively activate the enzyme in the enzyme solution and increase the activity of the enzyme, thereby decomposing the macromolecular substances and / or higher fatty acids in the tobacco raw material in subsequent treatment.
[0010] In some embodiments of the present application, in step (2), the mass of the enzyme preparation sprayed into the tobacco raw material is 0.1%-1% of the mass of the tobacco raw material.
[0011] In some embodiments of the present application, in step (3), the temperature is 30°C-50°C, and / or the relative humidity is 50%-90%, and / or the treatment time is 4h-24h. The temperature and / or humidity are set within the above ranges, which is conducive to the enzyme decomposing the macromolecular substances in the tobacco; the treatment time is set within the above ranges, which can make full use of the added enzyme solution and can at least reduce the sourness, spiciness and irritation of the heat-not-burn cigarette when smoking.
[0012] In some embodiments of the present application, in step (4), the temperature of the inactivation treatment is 80°C-120°C, and / or the time of the inactivation treatment is 10min-30min. Thus, the enzyme can be inactivated, so that the tobacco maintains good stability.
[0013] In some embodiments of the present application, in step (5), the temperature in the equilibrium environment is 22° C.-25° C., and the relative humidity is 60%-65%.
[0014] In some embodiments of the present application, the tobacco raw material includes at least one of tobacco flakes and tobacco shreds. The above method can be used to process tobacco flakes, tobacco shreds or a mixture of the two to reduce the sourness, spiciness or irritation of tobacco.
[0015] In some embodiments of the present application, the tobacco raw material includes at least one of flue-cured tobacco and air-cured tobacco. The above method can be used to process flue-cured tobacco, air-cured tobacco or a mixture of the two, using enzymes to decompose macromolecular substances in the flue-cured tobacco and / or air-cured tobacco, thereby reducing the sourness, spiciness or irritation of the tobacco during smoking. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:
[0017] Figure 1 A flow chart of a method for processing tobacco raw materials according to one embodiment of the present application is shown. DETAILED DESCRIPTION
[0018] The embodiments of the present application are described in detail below. The embodiments described below are exemplary and are only used to explain the present application, and should not be understood as limiting the present application.
[0019] Since heat-not-burn cigarettes have the characteristics of "heating tobacco or tobacco extracts instead of burning tobacco", the sensory quality of heat-not-burn cigarettes when smoking is quite different from that of traditional cigarettes. The smoke of heat-not-burn cigarettes made of unprocessed tobacco raw materials is strong, irritating, spicy, and strong sour, and has a heavy smell. Literature reports that there are more than 100 volatile and semi-volatile acidic components in tobacco, mainly C1-C8 volatile acids, higher fatty acids, etc., which have a great influence on the sensory quality of smoke. When heated, the smoke has a strong sour or sweet and sour taste; and if the content of macromolecular substances such as starch and protein in tobacco is too high, it will also produce a heavier spicy and irritating taste when heated. Therefore, reducing the content of macromolecular substances such as starch and protein in tobacco and reducing volatile and semi-volatile acidic components will correspondingly reduce the strong sour, spicy and irritating taste produced when heat-not-burn cigarettes are smoked, and improve the sensory quality of heat-not-burn cigarettes when smoking.
[0020] In view of this, in one aspect of the present application, the present application proposes a method for processing tobacco raw materials. In some embodiments of the present application, reference is made to Figure 1 The method for processing tobacco raw materials may include the following steps:
[0021] (1) An enzyme solution is formed, the pH value of the enzyme solution is adjusted, and then an activation treatment is performed.
[0022] In some embodiments of the present application, the enzyme powder is dissolved in water to form an enzyme solution, the pH value of the enzyme solution is adjusted to 6-8, and then an activation treatment can be performed at a temperature of 30°C-50°C (e.g., 30°C, 35°C, 40°C, 45°C, 50°C, etc.) to activate the enzyme. The activation temperature is within the range of 30°C-50°C, which can significantly increase the activity of the enzyme, thereby using the more active enzyme to process the tobacco raw material in the subsequent steps. The pH value of the enzyme solution is 6-8, which is conducive to increasing the activity of the enzyme.
[0023] In some embodiments of the present application, the enzyme preparation may include at least one of protease, amylase, saccharifying enzyme and cellulase, etc. The enzyme preparation can decompose macromolecules such as protein, starch, cellulose and the like in tobacco raw materials into small molecules, thereby reducing the content of macromolecules in tobacco, and can also utilize the catalytic effect of the enzyme preparation to degrade higher fatty acids in tobacco raw materials, thereby reducing the sourness, pungent taste or irritation of heat-not-burn cigarettes when smoking.
[0024] In some specific embodiments of the present application, the enzyme preparation can be a protease, an amylase, a saccharifying enzyme or a cellulase.
[0025] In some specific embodiments of the present application, the enzyme preparation may include at least two of protease, amylase, saccharifying enzyme and cellulase, etc., thereby being more conducive to reducing the content of macromolecular substances and / or higher fatty acids in tobacco, thereby further reducing the sourness, pungent taste or irritation of the heat-not-burn cigarette when smoking. In some embodiments of the present application, the enzyme preparation may include protease and amylase, or the enzyme preparation may include protease and cellulase, or the enzyme preparation may include amylase and cellulase, or the enzyme preparation may include saccharifying enzyme and protease, or the enzyme preparation may include saccharifying enzyme and cellulase, or the enzyme preparation may include protease, amylase and cellulase, or the enzyme preparation may include protease, amylase, saccharifying enzyme and cellulase.
[0026] In some embodiments of the present application, when the enzyme preparation includes a protease, the protease in the enzyme preparation may include at least one of a neutral protease and an alkaline protease. For example, the protease in the enzyme preparation may be a neutral protease or an alkaline protease, or a mixture of a neutral protease and an alkaline protease.
[0027] In some embodiments of the present application, the mass content of the enzyme preparation in the prepared enzyme solution can be 0.5%-5%, for example, the mass content of the enzyme preparation in the enzyme solution can be 0.5%, 0.8%, 1%, 1.5%, 2%, 3%, 4%, 5%, etc. The enzyme solution of the above concentration can be sprayed more evenly in the tobacco raw material, which is more conducive to the decomposition of macromolecular substances.
[0028] In some embodiments of the present application, the mass of the enzyme preparation sprayed into the tobacco raw material can be 0.1%-1% of the mass of the tobacco raw material. For example, the mass of the enzyme preparation can be 0.1%, 0.3%, 0.5%, 0.8%, 1% and the like of the mass of the tobacco raw material. Thus, the added amount of the enzyme preparation is within the above range. Through activation treatment, the enzyme can decompose the macromolecules in the tobacco raw material, effectively reduce the macromolecules in the tobacco raw material, and can also degrade the higher fatty acids in the tobacco raw material to a certain extent, reduce the content of higher fatty acids in the tobacco raw material, thereby reducing the sourness, spiciness or irritation of the heat-not-burn cigarette when smoking.
[0029] In some embodiments of the present application, in step (1), the pH value of the enzyme solution can be adjusted by adding at least one of citric acid and / or malic acid to the enzyme solution. Citric acid and malic acid can not only be used to adjust the pH value of the enzyme solution, but also will not adversely affect the smoking taste of the heat-not-burn cigarette. In some embodiments of the present application, citric acid can be added to adjust the pH value of the enzyme solution; in other embodiments of the present application, malic acid can be added to adjust the pH value of the enzyme solution.
[0030] In some embodiments of the present application, in step (1), the activation treatment time can be 15 min-2 h, for example, the activation treatment time can be 15 min, 25 min, 1 h, 1.5 h, 2 h, etc., thereby more fully stimulating the activity of the enzyme, thereby improving the effect of the enzyme solution treatment of tobacco raw materials.
[0031] In some embodiments of the present application, in step (1), the activation treatment can be carried out at a temperature of 35°C-45°C. For example, the activation treatment can be carried out at temperatures such as 35°C, 38°C, 40°C, 43°C, 45°C, etc. The temperature of the activation treatment is within the above range, which can effectively activate the activity of the enzyme and shorten the time of the activation treatment, thereby saving the processing time of the tobacco raw materials.
[0032] In some embodiments of the present application, in step (1), the activation treatment time can be 30 minutes to 1 hour. For example, the activation treatment time can be 30 minutes, 35 minutes, 45 minutes, 50 minutes, 1 hour, etc. Within the above time range, the enzyme preparation is more fully activated. In subsequent treatments, the macromolecular substances in the tobacco raw materials can be effectively decomposed, thereby improving the sensory quality of the heat-not-burn cigarette when smoking.
[0033] (2) Spraying the enzyme solution onto the tobacco raw material.
[0034] After the enzyme solution is activated, it is sprayed into the tobacco raw material.
[0035] In some embodiments of the present application, the tobacco raw material may include at least one of tobacco flakes and tobacco shreds. In some specific embodiments of the present application, the tobacco raw material may be tobacco flakes, and herein, tobacco flakes refer to tobacco raw materials that have been destemmed and redried. In other specific embodiments of the present application, the tobacco raw material may be tobacco shreds. In other specific embodiments of the present application, the tobacco raw material may be a mixture of tobacco flakes and tobacco shreds.
[0036] In some embodiments of the present application, the tobacco raw material may include at least one of flue-cured tobacco and air-dried tobacco. For example, the tobacco raw material may be flue-cured tobacco or air-dried tobacco, or a mixture of flue-cured tobacco and air-dried tobacco.
[0037] In some embodiments of the present application, when the tobacco raw material includes sun-cured tobacco, the sun-cured tobacco in the tobacco raw material may include at least one of burley tobacco, oriental tobacco and cigar tobacco. For example, the sun-cured tobacco in the tobacco raw material may be burley tobacco, oriental tobacco or cigar tobacco, or the sun-cured tobacco in the tobacco raw material may be a mixture of two or three of burley tobacco, oriental tobacco and cigar tobacco.
[0038] (3) The tobacco raw materials are placed in a constant temperature and humidity environment for processing.
[0039] The tobacco raw materials sprayed with the enzyme solution are placed in a constant temperature and humidity environment for treatment. The enzymes in the enzyme solution will degrade the macromolecular substances and / or higher fatty acids in the tobacco raw materials through catalysis, thereby reducing the sourness, spiciness or irritation of the heat-not-burn cigarettes when smoking. For example, protease can decompose the protein in the tobacco raw materials into small molecular amino acids, amylase can decompose the starch in the tobacco raw materials into monosaccharides, and sugar and amino acids can undergo Maillard reaction to increase the aroma components of tobacco; cellulase can decompose the cellulose in the tobacco raw materials.
[0040] In some embodiments of the present application, the temperature in the constant temperature and humidity environment can be 30°C-50°C, for example, the temperature can be 30°C, 35°C, 40°C, 45°C, 50°C, etc.; the relative humidity in the constant temperature and humidity environment can be 50%-90%, for example, the relative humidity can be 50%, 60%, 70%, 80%, 90%, etc.; the treatment time can be 4h-24h, for example, the treatment time can be 4h, 8h, 10h, 15h, 20h, 24h, etc. Under the above conditions, the enzyme can maintain a high activity and degrade macromolecular substances such as starch and protein and higher fatty acids in tobacco raw materials.
[0041] In some embodiments of the present application, in step (3), the temperature in the constant temperature and humidity environment can be 35°C-45°C, for example, the temperature can be 35°C, 37°C, 40°C, 42°C, 45°C, etc. The temperature is set within the above range, which is more conducive to the enzyme decomposing the macromolecular substances in the tobacco raw materials and is conducive to shortening the processing time.
[0042] In some embodiments of the present application, in step (3), the treatment time can be 4h-8h, for example, the treatment time can be 4h, 5h, 6h, 7h, 8h, etc. In a shorter period of time, the enzyme in the enzyme solution can effectively decompose the macromolecular substances in the tobacco raw material and reduce the content of the macromolecular substances, thereby reducing the sourness, spiciness and irritation of the heat-not-burn cigarette when smoking.
[0043] In some embodiments of the present application, the tobacco raw material after spraying the enzyme solution can be placed in a temperature and humidity control box, and the temperature and relative humidity in the temperature and humidity control box can be set so that the tobacco raw material is in a constant temperature and humidity environment. When inactivation treatment is required later, the temperature in the temperature and humidity control box can be adjusted.
[0044] (4) Perform inactivation treatment.
[0045] In some embodiments of the present application, after being treated in the temperature range of 30°C-50°C for a period of time, the tobacco raw material treated in step (3) can be inactivated at 80°C-120°C. For example, the temperature in the temperature and humidity control box can be adjusted to 80°C-120°C (for example, 80°C, 90°C, 100°C, 110°C, 120°C, etc.) for inactivation treatment to make the enzyme lose its biological activity. After the inactivation treatment is completed, the enzyme no longer acts on the tobacco raw material, so that the treated tobacco raw material has good stability.
[0046] In some embodiments of the present application, the inactivation treatment time can be 10min-30min, for example, the inactivation treatment time can be 10min, 15min, 20min, 25min, 30min, etc., thereby making the enzyme lose its biological activity and no longer decompose macromolecules in the tobacco raw materials.
[0047] In some embodiments of the present application, the temperature of the inactivation treatment can be 80°C-100°C, and the time of the inactivation treatment can be 10min-20min. For example, the temperature of the inactivation treatment can be 80°C, 85°C, 90°C, 95°C, 100°C, etc., and the time of the inactivation treatment can be 10min, 12min, 15min, 18min, 20min, etc. Thus, the inactivation treatment can be completed in a shorter time within a relatively low temperature range, so that the enzyme loses its biological activity, which is beneficial to saving tobacco processing costs.
[0048] (5) Perform balancing processing.
[0049] After the inactivation treatment, the tobacco raw material after the inactivation treatment is placed in a balanced environment for balanced treatment. In some embodiments of the present application, the temperature in the balanced environment can be 22°C-25°C, and the relative humidity can be 60%-65%. For example, the temperature can be 22°C, 23°C, 24°C, 25°C, etc., and the relative humidity can be 60%, 61%, 62%, 63%, 64%, 65%, etc. After the balanced treatment, the tobacco raw material has a suitable moisture content, a uniform moisture distribution, and a stable quality of the tobacco raw material.
[0050] By using the method proposed in this application to treat tobacco raw materials, the enzyme preparation can decompose part of the macromolecular substances such as protein, starch, cellulose and / or higher fatty acids in the tobacco raw materials, thereby reducing the sourness, spiciness and / or irritation of heat-not-burn cigarettes when smoking. The process flow required for this treatment process is relatively simple, the process equipment is mature, and it is suitable for large-scale production and application, which has practical significance for the production and development of heat-not-burn cigarette products.
[0051] The present application will be described below by specific examples, and it will be appreciated by those skilled in the art that the following specific examples are only for illustrative purposes, and do not limit the scope of the present application in any way. In addition, in the following examples, unless otherwise specified, the materials and equipment used are all commercially available. If in the following examples, specific processing conditions and processing methods are not clearly described, then conditions and methods known in the art can be used to process.
[0052] Example 1
[0053] 1) Protease, amylase and saccharifying enzyme are compounded in a mass ratio of 3:0.2:2, and water is added to prepare a composite enzyme solution with a mass content of 1% of the enzyme preparation, and anhydrous citric acid is added to the composite enzyme solution to adjust the pH value of the composite enzyme solution to 6, and then the composite enzyme solution is placed at a temperature of 40° C. for activation for 1 hour;
[0054] 2) spraying the complex enzyme solution prepared in step 1) uniformly into the tobacco raw material in an amount of 10% of the mass of the tobacco raw material, that is, the ratio of the mass of the complex enzyme solution to the mass of the tobacco raw material is 1:10, and the mass of the enzyme preparation added to the tobacco raw material is 0.1% of the mass of the tobacco raw material;
[0055] 3) placing the tobacco raw material prepared in step 2) in a constant temperature and humidity environment at a temperature of 40° C. and a relative humidity of 70% for 4 hours;
[0056] 4) placing the tobacco raw material obtained in step 3) in an environment of 100° C. for inactivation for 20 minutes;
[0057] 5) Placing the tobacco raw material inactivated in step 4) in a balanced environment for 48 hours (equilibrium conditions: temperature of 22° C., relative humidity of 60%) to obtain a pretreated tobacco raw material.
[0058] Example 2
[0059] The difference from Example 1 is that in Example 2, the complex enzyme solution is sprayed into the tobacco raw material in an amount of 100% of the mass of the tobacco raw material, that is, the ratio of the mass of the complex enzyme solution to the mass of the tobacco raw material is 1:1, the mass of the enzyme preparation added to the tobacco raw material is 1% of the mass of the tobacco raw material, and the remaining steps and parameters are the same as in Example 1.
[0060] Example 3
[0061] The difference from Example 2 is that in step 3) of Example 3, the treatment time in a constant temperature and humidity environment is 8 hours, and the remaining steps and parameters are the same as those in Example 2.
[0062] Application effect evaluation:
[0063] After adding smoke generating agents (such as propylene glycol, glycerol, etc.) to the tobacco raw materials obtained after treatment in Examples 1-3 and untreated burley tobacco raw materials (control samples) in the same proportion, sensory quality evaluation was performed using a heat-not-burn device. The evaluation results are shown in Table 1 below:
[0064] Table 1 Sensory quality evaluation results of each embodiment and control sample
[0065]
[0066] In Table 1, the sensory evaluation is compared:
[0067] Higher smoke concentration > Slightly higher smoke concentration > Low smoke concentration;
[0068] Moderate strength > moderate strength > strong strength;
[0069] Good clumping ability > fair clumping ability > poor clumping ability;
[0070] Smoke is relatively fine > Smoke is slightly fine > Smoke is rough and sour;
[0071] Better aroma quality > Slightly better aroma quality > Poor aroma quality;
[0072] The aroma is sufficient > the aroma is acceptable > the aroma is bland;
[0073] Slightly impure gas > Slightly impure gas > Heavy impurities;
[0074] Slightly irritating > Slightly irritating > Very irritating;
[0075] The aftertaste in the mouth is acceptable > the aftertaste in the mouth is slightly bad > the aftertaste in the mouth is strong and the aftertaste is bad;
[0076] Better comfort > Slightly worse comfort > Poor comfort.
[0077] As shown in Table 1, after the tobacco raw material is treated with the enzyme preparation, in the heated state, the smoke is mellow and moderate in strength, the impurities and irritation are significantly reduced, the sourness is significantly weakened, the sensory smoking quality is significantly improved, and the applicability in the heat-not-burn cigarette products is significantly enhanced. It should be noted that the amount of enzyme preparation added in Example 2 is relatively large, and the prepared tobacco raw material in step 3) is treated for a short time under a constant temperature and humidity environment. More enzyme preparations will bring a small amount of impurities, while in Example 3, the treatment time under a constant temperature and humidity environment is longer, and the amount of impurities is less.
[0078] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "other embodiments", "some specific embodiments", "other specific embodiments", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment are included in at least one embodiment of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.
[0079] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.
Claims
1. A method for processing tobacco raw materials, characterized in that: The following steps are involved: (1) dissolving the enzyme preparation powder in water to form an enzyme solution, adjusting the pH value of the enzyme solution, and then activating the enzyme solution; (2) spraying the activated enzyme solution into the tobacco raw material; (3) placing the tobacco raw material after spraying the enzyme solution in a constant temperature and humidity environment for treatment; (4) performing inactivation treatment on the tobacco raw material treated in step (3); (5) Placing the inactivated tobacco raw materials in a balanced environment for balanced treatment.
2. The method according to claim 1, characterized in that In step (1), the enzyme preparation includes at least one of protease, amylase, saccharifying enzyme and cellulase.
3. The method according to claim 1, characterized in that In step (1), the mass content of the enzyme preparation in the enzyme solution is 0.5%-5%.
4. The method according to claim 1, characterized in that In step (1), the pH value of the enzyme solution is adjusted to 6-8; And / or, in step (1), at least one of citric acid and / or malic acid is added to the enzyme solution to adjust the pH value of the enzyme solution.
5. The method according to claim 1, characterized in that In step (1), the temperature of the activation treatment is 30°C-50°C, and / or the time of the activation treatment is 15 min-2 h.
6. The method according to any one of claims 1 to 5, characterized in that In step (2), the mass of the enzyme preparation sprayed onto the tobacco raw material is 0.1%-1% of the mass of the tobacco raw material.
7. The method according to any one of claims 1 to 5, characterized in that In step (3), the temperature is 30° C.-50° C., and / or the relative humidity is 50%-90%, and / or the treatment time is 4 h-24 h.
8. The method according to any one of claims 1 to 5, characterized in that In step (4), the temperature of the inactivation treatment is 80°C-120°C, and / or the time of the inactivation treatment is 10min-30min.
9. The method according to any one of claims 1 to 5, characterized in that In step (5), the temperature in the equilibrium environment is 22° C.-25° C., and the relative humidity is 60%-65%.
10. The method according to any one of claims 1 to 5, characterized in that The tobacco raw material includes at least one of tobacco flakes and tobacco shreds; And / or, the tobacco raw material includes at least one of flue-cured tobacco and air-dried tobacco.