Monoclonal antibody and ELISA kit for detecting mycoplasma bovis

By using hybridoma cell lines 13B4 and 20H2, which secrete monoclonal antibodies against bovine mycoplasma, a double-antibody sandwich ELISA method was established, which solved the problem of rapid and high-throughput detection of bovine mycoplasma infection and achieved detection results with high sensitivity and strong specificity.

CN119955739BActive Publication Date: 2026-06-16SHIHEZI UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHIHEZI UNIVERSITY
Filing Date
2025-02-18
Publication Date
2026-06-16

AI Technical Summary

⚠Technical Problem

Existing technologies are insufficient for rapid and high-throughput detection of bovine mycoplasma infection, and there is a lack of effective prevention and control measures, which affects the development of the cattle industry.

⚗Method used

Hybridoma cell lines 13B4 and 20H2, which secrete monoclonal antibodies against bovine mycoplasma, were provided to establish a double-antibody sandwich ELISA detection method. 13B4 was used as the capture antibody and 20H2 was used as the detection antibody. The coating concentration and dilution were optimized, and HPR enzyme labeling was combined to establish a rapid and high-throughput detection method.

🎯Benefits of technology

It achieves rapid and sensitive detection of bovine mycoplasma, with a detection limit of 1.0×10⁵ CFU/ml. The method is highly specific, does not cross-react with other microorganisms, and has good batch-to-batch consistency and repeatability.

✦ Generated by Eureka AI based on patent content.

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Abstract

The application provides a monoclonal antibody and an ELISA kit for detecting Mycoplasma bovum, and belongs to the technical field of antigen detection. The application uses an antigen immunization method to screen two hybridoma cell strains stably secreting monoclonal antibodies, which are named as hybridoma cell strains 13B4 and 20H2 respectively, and the obtained monoclonal antibodies are named as monoclonal antibodies 13B4 and 20H2 respectively. The application establishes a double-antibody sandwich ELISA kit and a detection system for detecting Mycoplasma bovum, which has high specificity and sensitivity in detecting inactivated Mycoplasma bovum liquid, and the minimum detection is 1.0x10 5 CFU / ml, and has high sensitivity; and is suitable for detecting Mycoplasma bovum antigens, and provides an effective method for preventing and controlling Mycoplasma bovum.
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