Use of trans-2-hexenal as a weed germination inhibitor
Trans-2-hexenal, as a weed germination inhibitor, solves the problems of weed resistance and environmental pollution in wheat fields by soil injection or soil mixing, achieving efficient and safe weed germination inhibition and sowing safety.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
- Filing Date
- 2025-01-23
- Publication Date
- 2026-06-05
AI Technical Summary
Existing chemical herbicides have led to frequent occurrences of herbicide resistance in wheat fields and pose environmental pollution risks. There is an urgent need to develop green new products to control the germination of weeds in wheat fields.
Trans-2-hexenal was used as a weed germination inhibitor, applied by soil injection or soil mixing at a concentration of not less than 15 μL/L, to inhibit the germination of grass weeds in wheat fields, and wheat was sown at least 5 days after application.
It effectively suppresses the germination of grassy weeds in wheat fields, reduces the use of chemical herbicides, achieves green pest control, and has high sowing safety and a short sowing interval.
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Figure CN119969395B_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of agricultural pesticide technology, and in particular to the application of trans-2-hexenal as a weed germination inhibitor. Background Technology
[0002] Wheat is one of the major food crops. Weeds are the main pests threatening wheat yield and quality. Chemical herbicides are currently the primary technology for weed control in wheat fields. However, with changes in planting systems, industrial restructuring, and the use of herbicides, wheat field weed communities are undergoing succession, with herbicide-resistant and difficult-to-control weeds such as jointed goatgrass gradually becoming dominant weeds. The unscientific use of herbicides year after year has also led to frequent herbicide resistance in wheat field weeds. For example, studies have reported that wheat field weeds such as Japanese bluegrass, large-spike bluegrass, and ryegrass have developed varying degrees of resistance to herbicides with different mechanisms of action, increasing the difficulty of weed control in wheat fields. Furthermore, during herbicide use, some herbicide residues pollute water bodies, the atmosphere, and soil, posing a potential threat to human health. Based on these problems, there is an urgent need to develop new green products and technologies for controlling wheat field weeds.
[0003] Trans-2-hexenal, also known as leaf aldehyde, has the molecular formula C6H. 10 O, is an aliphatic unsaturated olefin aldehyde, belonging to the category of green volatiles. Most green plants can rapidly synthesize and release it after being attacked. trans-2-hexenal appears as a pale yellow to colorless oily liquid with a faint fruity aroma, mainly found in peaches, cucumbers, apples, as well as camphor oil and citronella oil. Industrially, trans-2-hexenal is primarily produced through chemical synthesis rather than isolation from natural sources. The World Health Organization's Expert Committee on Food Additives has assessed trans-2-hexenal as a food-grade flavoring additive safe for human consumption and can be used to enhance food flavor. Trans-2-hexenal possesses certain antibacterial activity and is widely used in fruit and vegetable preservation. Furthermore, trans-2-hexenal exhibits good fumigation toxicity against *Aphis leekus* and strong nematicidal activity against pine wood nematodes, wheat root-knot nematodes, and wheat cyst nematodes. Currently, there are no reports of using trans-2-hexenal as a pre-emergence weed germination inhibitor to reduce soil weeds.
[0004] In view of this, the present invention is hereby proposed. Summary of the Invention
[0005] To address the aforementioned technical problems, this invention provides the application of trans-2-hexenal as a weed germination inhibitor. As a novel weed control method, it can efficiently, non-toxically, and safely eliminate the seed bank of weeds in wheat fields from the source.
[0006] Specifically, the technical solution of the present invention is as follows:
[0007] In a first aspect, the present invention provides an application of trans-2-hexenal as a weed germination inhibitor in wheat fields: trans-2-hexenal inhibits the germination of weeds in wheat fields at a concentration of 15 μL / L or higher.
[0008] Preferably, the weeds in the wheat field are grassy weeds.
[0009] Preferably, the wheat field weeds include at least one of jointed goatgrass, columnar goatgrass, Japanese barnyardgrass, large spike barnyardgrass, and ryegrass germination.
[0010] Preferably, the wheat field weeds include jointed goatgrass, and the concentration of trans-2-hexenal, which inhibits the germination of jointed goatgrass, is 15 μL / L or higher; more preferably 38 μL / L or higher; and more preferably 38-76 μL / L.
[0011] Preferably, the wheat field weeds include at least one of *Aegilops cylindrica*, *Alopecurus japonicus*, *Alopecurus thunbergii*, and *Lerium ryegrass*, and the concentration of trans-2-hexenal that inhibits the germination of *Aegilops cylindrica*, *Alopecurus japonicus*, *Alopecurus thunbergii*, or *Lerium ryegrass* is 38 μL / L or higher.
[0012] Preferably, trans-2-hexenal is applied to the field by soil injection or by mixing with soil.
[0013] Preferably, the depth of soil injection is 2±1cm.
[0014] Preferably, the concentration of the trans-2-hexenal solution injected into the soil is 38 μL / L or higher, and more preferably 38-76 μL / L.
[0015] Preferably, the amount of trans-2-hexenal solution used in the soil injection is 42 μL / kg or more, and more preferably 42-84 μL / kg.
[0016] Preferably, the depth of the soil mixture is 10±1cm.
[0017] Preferably, the concentration of the trans-2-hexenal solution in the soil mixture is 38 μL / L or higher, and more preferably 38-76 μL / L.
[0018] Preferably, the amount of trans-2-hexenal solution used in the soil mixing is 42 μL / kg or more, and more preferably 42-84 μL / kg.
[0019] Preferably, wheat should be sown at least 5 days after the application of trans-2-hexenal in the field.
[0020] Preferably, wheat is sown again after an interval of 5-20 days.
[0021] Secondly, the present invention provides a method for suppressing weeds in wheat fields, using trans-2-hexenal as a weed germination inhibitor in wheat fields, and after applying trans-2-hexenal in the field, wheat should be sown again after an interval of at least 5 days.
[0022] Beneficial effects:
[0023] This invention is the first to discover that trans-2-hexenal has an inhibitory effect on the germination of grassy weeds in wheat fields at a certain concentration, thus providing an application for trans-2-hexenal as a weed germination inhibitor. This invention also provides an optimal application method of trans-2-hexenal in soil and clarifies the safe interval for wheat sowing after the application of trans-2-hexenal. Therefore, trans-2-hexenal can be used to reduce the seed bank of grassy weeds in wheat fields, controlling weed occurrence at the source, achieving green weed control in wheat fields, and reducing the use of chemical herbicides. Attached Figure Description
[0024] To more clearly illustrate the technical solutions in this invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be described below.
[0025] Figure 1 This study shows the effect of different concentrations of trans-2-hexenal on the germination of *Gnaphalium affine*.
[0026] Figure 2 This study demonstrates the inhibitory effect of trans-2-hexenal on the germination of grassy weeds in wheat fields.
[0027] Figure 3 This study demonstrates the inhibitory effect of trans-2-hexenal on the germination of *Gnaphalium affine* under different application methods.
[0028] Figure 4 This shows the emergence of wheat seedlings at different intervals after the application of trans-2-hexenal. Detailed Implementation
[0029] This invention provides the application of trans-2-hexenal as a weed germination inhibitor in wheat fields. As a novel weed control method, injecting trans-2-hexenal into the soil can inhibit the germination of grassy weeds in wheat fields, reducing the seed bank of weeds in the soil at the source. It has the advantages of high efficiency, easy volatility, non-toxicity, and no residue. Moreover, the safe interval for sowing wheat after trans-2-hexenal soil injection is short, and wheat can be sown after 5 days, demonstrating a certain degree of safety.
[0030] First, the present invention provides a novel use for trans-2-hexenal.
[0031] Specifically, this invention provides a novel use of trans-2-hexenal as a weed germination inhibitor.
[0032] Specifically, trans-2-hexenal is used to inhibit the germination of grassy weeds in wheat fields.
[0033] Specifically, trans-2-hexenal is used to inhibit the germination of grass weeds such as jointed goatgrass, columnar goatgrass, Japanese barnyardgrass, large spike barnyardgrass, and ryegrass in wheat fields.
[0034] Specifically, in order to achieve the effect of inhibiting weed germination, the concentration of trans-2-hexenal is at least 15 μL / L, and preferably, the concentration of trans-2-hexenal is at least 38 μL / L.
[0035] Specifically, for *Gnaphalium affine*, to achieve a good germination inhibition effect, the concentration of trans-2-hexenal should be at least 15 μL / L. At this concentration, trans-2-hexenal has a significant germination inhibitory effect on *Gnaphalium affine*. The optimal concentration of trans-2-hexenal is at least 38 μL / L. At this concentration, trans-2-hexenal completely inhibits the germination of *Gnaphalium affine* seeds.
[0036] Specifically, for Aegilops cylindrica, Alopecurus japonicus, Alopecurus macrospinipes, and Lorraine ryegrass, this invention verified that the optimal concentration of trans-2-hexenal is at least 38 μL / L. At this concentration, trans-2-hexenal completely inhibits the seed germination of Aegilops cylindrica, Alopecurus japonicus, Alopecurus macrospinipes, and Lorraine ryegrass.
[0037] This invention further provides a method for applying trans-2-hexenal in soil.
[0038] Specifically, trans-2-hexenal is applied to soil via soil injection and / or mixing. Preferably, trans-2-hexenal is applied via soil injection.
[0039] Specifically, the injection depth of trans-2-hexenal in the soil was 2 cm.
[0040] Specifically, to achieve the effect of inhibiting weed germination, taking jointed goatgrass as an example, the soil injection concentration of trans-2-hexenal is at least 38 μL / L, preferably at least 76 μL / L.
[0041] Specifically, to suppress weed germination, taking jointed goatgrass as an example, the concentration of trans-2-hexenal in the soil should be at least 38 μL / L.
[0042] This invention further investigates and clarifies the safe interval for wheat sowing after trans-2-hexenal soil injection.
[0043] Specifically, sowing wheat at least 5 days after trans-2-hexenal soil injection has no significant effect on wheat emergence.
[0044] Specifically, sowing wheat 5-20 days after soil injection of trans-2-hexenal has no significant effect on wheat emergence.
[0045] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.
[0046] The endpoints and any values of the ranges disclosed in this specification are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of the various ranges, the endpoint values of the various ranges and individual point values, and individual point values can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.
[0047] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "specific implementation," or "some specific implementations," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.
[0048] In the embodiments provided in this specification, unless specific techniques or conditions are specified, the techniques or conditions described in the literature in this field, or the product instructions, shall be followed. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased from legitimate channels (trans-2-hexenal (original concentration) was obtained from commercially available sources).
[0049] Example 1
[0050] This embodiment investigated the effect of trans-2-hexenal on the germination of Jointed Gynostemma pentaphyllum.
[0051] Select uniformly sized jointed goatgrass seeds and rinse them three times with sterile water before use. Use 9 cm diameter circular plastic petri dishes as containers for the experiment. Place a double layer of filter paper at the bottom of the petri dish, moisten the filter paper with 5 mL of sterile water, and evenly arrange 20 jointed goatgrass seeds on the double layer of filter paper. Take the bottom of another petri dish and fix a 2 cm long piece of positively oriented filter paper to the center of the bottom with double-sided tape. Add different volumes of trans-2-hexenal to the filter paper piece. Then, invert the two petri dishes together and seal them with sealing film. The concentrations of trans-2-hexenal were set to 0, 3.8, 7.6, 15, 38, and 60 μL / L. After the operation, place all petri dishes in an artificial climate incubator (Zhejiang Top Instrument Co., Ltd.), with the temperature set at 25 / 22℃ (light / dark), the light cycle at L / D = 14 h / 10 h, and the light intensity at 320 μmol / m². 2 After 96 hours of cultivation, the number of germinated jointed wheat was counted, and germination was defined as when the radicle length was greater than 3 mm.
[0052] The above experiment was repeated four times. The results are shown below. Figure 1 .
[0053] Figure 1 The results showed that 3.8 μL / L and 7.6 μL / L trans-2-hexenal had no significant effect on the germination of jointed goatgrass seeds. Concentrations of 15, 38, and 60 μL / L trans-2-hexenal significantly inhibited the germination of jointed goatgrass seeds, with concentrations of 38 μL / L and 60 μL / L completely inhibiting germination.
[0054] Example 2
[0055] This embodiment investigated the inhibitory effect of trans-2-hexenal on the germination of grass weeds in wheat fields.
[0056] Common grasses in wheat fields, including *Aegilops cylindrica*, *Lolium ryegrass*, *Alopecurus japonicus*, and *Alopecurus aequalis*, were used as test targets. Seeds of uniform size were selected and rinsed three times repeatedly with sterile water before use. The concentrations of trans-2-hexenal were set at 0 μL / L and 38 μL / L, with other procedures following the same procedures as in Example 1. After 96 h of cultivation, the number of germinations of *Aegilops cylindrica*, *Lolium ryegrass*, *Alopecurus japonicus*, and *Alopecurus aequalis* were counted, with germination defined as a radicle length greater than 3 mm.
[0057] The above experiment was repeated four times. The results are shown below. Figure 2 .
[0058] Figure 2The results showed that a concentration of trans-2-hexenal of 38 μL / L significantly inhibited the germination of seeds of Aegilops cylindrica, Lorrax ryegrass, Alopecurus japonicus and Alopecurus macrospinipes. The germination of seeds of Aegilops cylindrica, Lorrax ryegrass and Alopecurus japonicus was completely inhibited, while the germination rate of Alopecurus macrospinipes was less than 5%.
[0059] Example 3
[0060] This embodiment studies the effects of different applications of trans-2-hexenal on the germination of jointed goatgrass.
[0061] Select jointed barley seeds of uniform size for later use.
[0062] Garden soil that had not been sprayed with any herbicides was used in this experiment and was sieved before use.
[0063] Soil injection treatment: Sifted soil and organic fertilizer were mixed at a ratio of 3:1 (v / v) and filled into plastic flowerpots with a diameter of 11 cm and a height of 10 cm. 30 grains of jointed goatgrass were sown in each pot at a depth of 3 cm. Using a disposable sterile syringe (needle removed), trans-2-hexenal was drawn and injected into the center of the flowerpot to a depth of 2 cm. The injection volumes were 0, 25, and 50 μL, corresponding to trans-2-hexenal concentrations of 0, 38, and 76 μL / L, respectively. After the procedure, all flowerpots were placed in an artificial climate incubator with a temperature of 25 / 22℃ (light / dark), a light cycle of L / D = 14 h / 10 h, and a light intensity of 320 μmol / m². 2 s. The number of jointed goatgrass seedlings was counted 7 days after treatment. The above experiment was repeated 4 times.
[0064] Soil mixing treatment: The sieved soil is first mixed with organic fertilizer at a ratio of 3:1 (v / v), then trans-2-hexenal is added and mixed thoroughly. The mixed soil is then used to fill plastic flowerpots with a diameter of 11 cm and a height of 10 cm. 30 grains of jointed goatgrass are sown in each pot at a depth of 3 cm. The concentrations of trans-2-hexenal are set at 0, 38, and 76 μL / L, corresponding to application rates of 0, 25, and 50 μL per pot, respectively. After the operation, all flowerpots are placed in an artificial climate incubator with a temperature of 25 / 22℃ (light / dark), a light cycle of L / D = 14 h / 10 h, and a light intensity of 320 μmol / m². 2 s.
[0065] The number of jointed goatgrass seedlings was counted 7 days after treatment.
[0066] The above experiment was repeated four times. The results are shown below. Figure 3 .
[0067] Figure 3The results showed that both soil injection and soil mixing with trans-2-hexenal significantly inhibited the germination of *Gnaphalium affine* seeds. Under soil injection, the inhibitory effect of trans-2-hexenal at a concentration of 76 μL / L was significantly higher than that at 38 μL / L, with a seedling emergence rate of 11.7%. Under soil mixing, the inhibitory effect of trans-2-hexenal at 76 μL / L was not significantly different from that at 38 μL / L, and the seedling emergence rate of *Gnaphalium affine* was higher than 40% at both treatment concentrations.
[0068] Example 4
[0069] This embodiment studies the safe interval for wheat sowing.
[0070] The wheat variety is AK58.
[0071] Garden soil that had not been sprayed with any herbicides was used in this experiment and was sieved before use.
[0072] The sieved soil and organic fertilizer were thoroughly mixed at a ratio of 3:1 (v / v). The mixed soil was then used to fill plastic flowerpots with a diameter of 11 cm and a height of 10 cm. Using a disposable sterile syringe, trans-2-hexenal was injected into the center of the flowerpot to a depth of 2 cm, with a volume of 50 μL and a concentration of 76 μL / L. Wheat was sown 0, 5, 10, 15, and 20 days after trans-2-hexenal injection, with 30 seeds sown per pot at a depth of 3 cm. A blank control group was injected with an equal volume of sterile water, and wheat was sown 0 days after injection, with 30 seeds sown per pot at a depth of 3 cm. After the procedure, all flowerpots were placed in an artificial climate incubator with a temperature of 25 / 22℃ (light / dark), a light / dark cycle of L / D = 14 h / 10 h, and a light intensity of 320 μmol / m². 2 s. The number of jointed goatgrass seedlings was counted 7 days after treatment with trans-2-hexenal.
[0073] The above experiment was repeated four times. The results are shown below. Figure 4 .
[0074] Figure 4 The results showed that wheat sown 0 days after trans-2-hexenal injection had a seedling emergence rate of 1.67%, significantly lower than the control treatment. Wheat sown 5, 10, 15, and 20 days after trans-2-hexenal injection showed no significant difference in seedling emergence rate compared to the control treatment.
[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. The application of trans-2-hexenal as a weed germination inhibitor in wheat fields, characterized in that, trans-2-hexenal inhibits the germination of weeds in wheat fields, wherein the wheat field weeds are selected from at least one of jointed goatgrass, chebulic aegypti, Japanese barnyardgrass, large spike barnyardgrass, and ryegrass. Among them, the concentration of trans-2-hexenal that inhibits the germination of jointed goatgrass is above 15 μL / L; The concentration of trans-2-hexenal that inhibits the germination of Aegilops cylindrica, Alopecurus japonicus, Alopecurus thunbergii, or Lernaea ryegrass is above 38 μL / L.
2. The application according to claim 1, characterized in that, The concentration of trans-2-hexenal that inhibits jointed goatgrass germination is above 38 μL / L.
3. The application according to claim 2, characterized in that, The concentration of trans-2-hexenal that inhibits jointed goatgrass germination is 38-76 μL / L.
4. The application according to claim 1, characterized in that, Trans-2-hexenal was applied to the field by soil injection or by mixing with soil.
5. The application according to claim 4, characterized in that, The depth of the soil injection is 2±1cm; The concentration of the trans-2-hexenal solution injected into the soil is above 38 μL / L.
6. The application according to claim 5, characterized in that, The concentration of the trans-2-hexenal solution injected into the soil was 38-76 μL / L.
7. The application according to claim 4, characterized in that, The depth of the soil mixture is 10±1cm; The concentration of the trans-2-hexenal solution in the soil mixture is above 38 μL / L.
8. The application according to claim 7, characterized in that, The concentration of the trans-2-hexenal solution in the soil mixture is 38-76 μL / s.
9. The application according to any one of claims 1-7, characterized in that, After applying trans-2-hexenal in the field, wait at least 5 days before sowing wheat.
10. The application according to claim 9, characterized in that, After applying trans-2-hexenal in the field, wait 5-20 days before sowing wheat.