Escherichia coli engineering strain for producing 1,3-dihydroxyacetone by utilizing glucose

Abstract

The application discloses an Escherichia coli engineering bacterium for producing 1,3-dihydroxyacetone by using glucose. The application provides the Escherichia coli engineering bacterium for producing 1,3-dihydroxyacetone by using glucose as a carbon source, which is Escherichia coli TZ-249, and the preservation number of the Escherichia coli TZ-249 in the China General Microbiological Culture Collection Center is CGMCC No. 26177. The application has important significance for producing bulk chemicals 1,3-dihydroxyacetone by using glucose as a raw material.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to an engineered Escherichia coli strain that utilizes glucose to produce 1,3-dihydroxyacetone. Background Art

[0002] 1,3-Dihydroxyacetone (DHA) is the simplest three-carbon ketose with three active groups. It can participate in a variety of reactions and is widely used in the chemical, pharmaceutical, feed, cosmetic and food industries. The main methods for synthesizing DHA are chemical synthesis and microbial synthesis. Due to the disadvantages of chemical synthesis such as low conversion efficiency, selective oxidation efficiency, and high catalyst cost, researchers prefer to use microbial methods with optimized environment and higher conversion efficiency to synthesize DHA. At present, the industrial production of DHA mainly uses Gluconobacter oxydans to convert glycerol substrates, but there are also problems such as high concentrations of glycerol substrates and DHA products inhibiting bacterial growth, by-products and complex fermentation processes. In recent years, with the technological advancement in the field of synthetic biology, the construction of efficient microbial cell factories to synthesize DHA using glucose as a substrate has gradually become a research trend. Summary of the Invention

[0003] The purpose of the present invention is to provide an engineered Escherichia coli strain that utilizes glucose to produce 1,3-dihydroxyacetone.

[0004] In a first aspect, the present invention provides Escherichia coli TZ-249, whose deposit number is CGMCC No.26177.

[0005] In a second aspect, the present invention provides a bacterial agent, the active ingredient of which is the Escherichia coli TZ-249 or its bacterial liquid or culture liquid or fermentation liquid described in the first aspect.

[0006] In addition to the active ingredients, the bacterial agent may also contain auxiliary materials or carriers commonly used in the art.

[0007] In a third aspect, the present invention provides the use of TZ-249 or its bacterial solution or culture fluid or fermentation fluid described in the first aspect, or the bacterial agent described in the second aspect, in at least one of the following:

[0008] 1) Production of 1,3-dihydroxyacetone;

[0009] 2) Increase the yield of 1,3-dihydroxyacetone;

[0010] 3) Increase the production rate of 1,3-dihydroxyacetone;

[0011] 4) Promote the conversion of glucose into 1,3-dihydroxyacetone.

[0012] In the above application, the production of 1,3-dihydroxyacetone is to use glucose as a carbon source to produce 1,3-dihydroxyacetone.

[0013] In a fourth aspect, the present invention provides a method for producing 1,3-dihydroxyacetone, comprising the following steps: fermenting and culturing the Escherichia coli TZ-249 described in the first aspect to obtain 1,3-dihydroxyacetone.

[0014] In the above method, the fermentation culture utilizes glucose as a carbon source to produce 1,3-dihydroxyacetone.

[0015] Furthermore, the fermentation medium used in the fermentation culture can be a semi-synthetic medium CM9 containing 50 g / L glucose.

[0016] In a specific embodiment of the present invention, the fermentation medium is formulated as follows: per liter, it contains 50 g of glucose, 5 g of corn steep liquor, 1 g of NH4Cl, 0.87 g of (NH4)2SO4, 0.5 g of MgSO4·7H2O, 0.47 g of KCl, 0.14 g of KH2PO4, and trace elements: 1.47 μM ZnCl2, 0.81 μM H3BO3, 0.84 μM CoCl2·6H2O, 0.59 μM CuCl2·2H2O, 5.92 μM FeCl3·6H2O, 0.83 μM Na2MoO4·2H2O, with the balance being water. During the fermentation culture, 700 g / L of glucose was used as feed, and aqueous ammonia was used as a neutralizer. The fermentation conditions are as follows: temperature 37°C, pH 7.0, dissolved oxygen 30%, upper limit of ventilation flow rate 10 L / min, lower limit 3 L / min, upper limit of stirring speed 1000 r / min, lower limit 300 r / min, and after inoculation, the dissolved oxygen content is controlled by controlling the dissolved oxygen, stirring speed, and ventilation cascade.

[0017] The starting strain in the present invention is the engineered Escherichia coli TZ-237, which produces 1,3-dihydroxyacetone using glucose as a carbon source. However, due to the non-enzymatic chemical reaction between intracellular and extracellular 1,3-dihydroxyacetone and phosphate to produce methylglyoxal, this inhibits and interferes with the physiological metabolism of the cell, affecting the growth, glucose metabolism, and DHA production of the engineered 1,3-dihydroxyacetone-producing strain. To improve strain growth and increase 1,3-dihydroxyacetone production, the engineered 1,3-dihydroxyacetone-producing strain required in-depth tolerance modification and strain screening, resulting in the recombinant Escherichia coli TZ-249 strain of the present invention.

[0018] Experiments conducted in the present invention demonstrate that, in a 5L fermentation over 30 hours using recombinant E. coli TZ-237, 1,3-dihydroxyacetone production reached 30.1 g / L, a production rate of 1 g / L / h, and a glucose-to-1,3-dihydroxyacetone conversion rate of 0.95 mol / mol. In contrast, in a 5L fermentation over 30 hours using recombinant E. coli TZ-249, 1,3-dihydroxyacetone production reached 83.7 g / L, a production rate of 2.79 g / L / h, and a glucose-to-1,3-dihydroxyacetone conversion rate of 0.96 mol / mol. This demonstrates that, starting from recombinant E. coli TZ-237, the recombinant E. coli TZ-249, derived through evolutionary metabolism, significantly improves its 1,3-dihydroxyacetone production capacity and production rate. The present invention's use of glucose as a substrate for 1,3-dihydroxyacetone production is of great significance.

[0019] Preservation Instructions

[0020] Strain name: Escherichia coli

[0021] Latin name: Escherichia coli

[0022] Reference biomaterial strain: TZ-249

[0023] Depository: General Microbiology Center of China Culture Collection Administration

[0024] Abbreviation of depository institution: CGMCC

[0025] Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

[0026] Deposit date: December 8, 2022

[0027] CGMCC registration number: CGMCC No.26177 BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 To obtain recombinant Escherichia coli TZ-249 through evolutionary metabolism, metabolic evolution was performed in a culture medium containing 20-50 g / L 1,3-dihydroxyacetone to improve the strain's tolerance to 1,3-dihydroxyacetone.

[0029] Figure 2 Recombinant E. coli TZ-237 was fermented in a 5L fermentor. ▲ represents the glucose concentration in the fermentation broth; ● represents the OD600nm value of the culture broth; ■ represents the 1,3-dihydroxyacetone concentration in the fermentation broth. The unit g / L represents the target compound content per liter of fermentation broth.

[0030] Figure 3Recombinant E. coli TZ-249 was fermented in a 5L fermentor. ▲ represents the glucose concentration in the fermentation broth; ● represents the OD600nm value of the culture broth; ■ represents the 1,3-dihydroxyacetone concentration in the fermentation broth. The unit g / L represents the target compound content per liter of fermentation broth. DETAILED DESCRIPTION

[0031] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0032] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0033] The Gene IDs of the genes used in the following examples are all from the NCBI database (all gene ID information is from the NCBI database, web link: https: / / www.ncbi.nlm.nih.gov / nuccore / CP000946.1 / ). Specifically, the nucleotide sequence of the gene encoding the endogenous glucose-specific PTS enzyme IIBC component PtsG (i.e., the ptsG gene) is Gene ID: EcolC_2500 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous pyruvate formate lyase PflB (i.e., the pflB gene) is Gene ID: EcolC_2693 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the pyruvate oxidase PoxB (i.e., the poxB gene) is Gene ID: EcolC_2725 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding endogenous alcohol dehydrogenase AdhE (ie, adhE gene) is Gene ID: EcolC_2387 (submission date: February 14, 2008).

[0034] The nucleotide sequence of the gene encoding the endogenous histidine phosphate carrier protein PtsH (i.e., the ptsH gene) is Gene ID: EcolC_1263 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous phosphoenolpyruvate-protein phosphotransferase IPtsI (i.e., the ptsI gene) is Gene ID: EcolC_1262 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous glucose-specific enzyme II complex EIIA Crr (i.e., the crr gene) is Gene ID: EcolC_1261 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous dihydroxyacetone kinase DhaRKLM (i.e., the dhaRKLM gene) is Gene ID: EcolC_2425 (submitted on February 14, 2008), EcolC_2426 (submitted on February 14, 2008), EcolC_2427 (submitted on February 14, 2008), and EcolC_2428 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous glycerol kinase GlpK (i.e., the glpK gene) is Gene ID: EcolC_4092 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the methylglyoxal synthase MgsA (i.e., the mgsA gene) is Gene ID: EcolC_2633 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding glycerol dehydrogenase GldA (i.e., gldA gene) is Gene ID: EcolC_4070 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding L-arabinose isomerase AraA (i.e., araA gene) is Gene ID: EcolC_3595 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding ribulose kinase AraB (i.e., araB gene) is Gene ID: EcolC_3594 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding endogenous triosephosphate isomerase TpiA (i.e., tpiA gene) is Gene ID: EcolC_4099 (submitted on February 14, 2008).

[0035] The nucleotide sequence of the gene encoding the endogenous fructose-6-phosphate aldolase 1 (FsaA) (i.e., the fsaA gene) is Gene ID: EcolC_2819 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous fructose-6-phosphate aldolase 2 (FsaB) (i.e., the fsaB gene) is Gene ID: EcolC_4069 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous glycerol transporter GlpF (i.e., the glpF gene) is Gene ID: EcolC_4091 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous glucose-6-phosphate dehydrogenase (Zwf) (i.e., the zwf gene) is Gene ID: EcolC_1780 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous phosphogluconate dehydratase Edd (i.e., the edd gene) is Gene ID: EcolC_1781 (submitted on February 14, 2008). The nucleotide sequence of the gene encoding the endogenous 2-keto-3-deoxy-6-phosphogluconate aldehyde Eda (i.e., the eda gene) is Gene ID: EcolC_1782 (submitted on February 14, 2008). The above Gene IDs are all Gene IDs in the NCBI database.

[0036] The Escherichia coli strain TZ-237 involved in the following examples is a strain of Escherichia coli ATCC 8739, which is a strain of Escherichia coli comprising the full-length ptsHI-crr gene (ptsHI-crr gene positions 1-2622, the ptsHI-crr gene consists of three genes, ptsH, ptsI and crr, and the sequence numbers of the three genes are Gene ID: EcolC_1261, EcolC_1262, and EcolC_1263), the full-length dhaRKLM gene (dhaRKLM gene positions 1-5290, the dhaRKLM gene consists of four genes, dhaR, dhaK, dhaL and dhaM, and the sequence numbers of the four genes are Gene ID: EcolC_2425, EcolC_2426, EcolC_2427, and EcolC_2428), the glpK gene (Gene ID: EcolC_4092) positions 1-1509, the mgsA gene (Gene ID: The 1st to 459th positions of the gldA gene (Gene ID: EcolC_2633), the 46th to 1104th positions of the gldA gene (Gene ID: EcolC_2633), the 274th to 3159th positions of the araBA gene (the araBA gene is composed of the araB gene and the araA gene, and the knockout part is from the 274th position of the araB gene to the end of the araA gene; the two genes are Gene ID: EcolC_3594, EcolC_3595) and the 1st to 758th positions of the tpiA gene (Gene ID: EcolC_4099) were knocked out to inactivate the functions of these genes, and P 93 -T7RNAP fragment (SEQ ID No. 1 No. 78-2682) replaced the fragment shown in position 151-1434 of ptsG gene in ATCC 8739 genome, and poxB::P T7 -hdpA fragment (SEQ ID No. 2 56-1158) replaced the fragment of poxB gene 396-1083 in ATCC 8739 genome, and pflB::P T7 -hdpA fragment (SEQ ID No. 4, positions 56-1158) replaced the fragment shown in positions 1-2283 of the pflB gene in the ATCC 8739 genome, and knocked out positions 1-663 of the fsaA gene and positions 1-663 of the fsaB gene, and positions 1-813 of the glpF gene, so that P 46 -nox (SEQ ID No. 3, positions 86-1621) replaced the 102-2676 fragment of the adhE gene of Escherichia coli; knocked out positions 1-1476 of the zwf gene, knocked out positions 1-1812 of the edd gene, and knocked out positions 1-642 of the eda gene to obtain recombinant bacteria.

[0037] The genotype of Escherichia coli strain TZ-237 is shown in Table 1.

[0038] Table 1 shows the strains used in the present invention.

[0039]

[0040] Example 1: Obtaining recombinant Escherichia coli TZ-249 through evolutionary metabolism

[0041] Starting from recombinant Escherichia coli TZ-237, the tolerance to 1,3-dihydroxyacetone was improved through evolutionary metabolism, thereby improving the strain's ability to produce 1,3-dihydroxyacetone.

[0042] Seed culture medium (1 L): 5 g yeast extract, 10 g tryptone, 10 g NaCl, and the balance water.

[0043] CM9 fermentation medium (1 L): glucose 50 g, corn steep liquor 5 g, NH4Cl 1 g, (NH4)2SO4 0.87 g, MgSO4·7H2O 0.5 g, KCl 0.47 g, KH2PO4 0.14 g, and trace elements ZnCl2 1.47 μM, H3BO3 0.81 μM, CoCl2·6H2O 0.84 μM, CuCl2·2H2O 0.59 μM, FeCl3·6H2O 5.92 μM, Na2MoO4·2H2O 0.83 μM, and the balance is water.

[0044] Acclimation medium: Add 20-50 g / L (concentration in acclimation medium) of 1,3-dihydroxyacetone (DHA) to the CM9 fermentation medium to obtain an acclimation medium with DHA concentration gradually increasing from 20 g / L to 50 g / L.

[0045] Acclimation Culture: Transfer the recombinant E. coli TZ-237 monoclonal from the LB plate to 2 ml of seed culture medium and culture at 37°C, 250 rpm for 18 hours to obtain seed culture solution. The seed culture solution is then transferred to 50 ml of acclimation medium containing 20 g / L to 50 g / L 1,3-dihydroxyacetone at a 1% (volume ratio) inoculation rate. During the acclimation process, the 1,3-dihydroxyacetone level is increased from 20 g / L, 25 g / L, 30 g / L, 33 g / L, 36 g / L, 38 g / L, 40 g / L, 42 g / L, 44 g / L, 45 g / L, 46 g / L, 47 g / L, 48 g / L, 49 g / L to 50 g / L.

[0046] After 81 generations of transfer culture ( Figure 1), and finally obtained strain TZ-249 that grew well in the acclimation medium containing 50 g / L 1,3-dihydroxyacetone.

[0047] Strain TZ-249 was deposited in the China General Microorganism Culture Collection Center (CGMCC, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing) on ​​December 8, 2022. Its deposit number is CGMCC No. 26177, and the recommended classification name is Escherichia coli.

[0048] Example 2: Shake flask fermentation of recombinant Escherichia coli TZ-237 and TZ-249

[0049] The recombinant Escherichia coli TZ-237 and TZ-249 were evaluated for fermentation production of 1,3-dihydroxyacetone.

[0050] Seed culture medium: same as in Example 1.

[0051] CM9 fermentation medium: same as in Example 1.

[0052] Shake flask fermentation: Recombinant E. coli TZ-237 and TZ-249 clones isolated from LB plates were inoculated into 2 ml of seed culture medium and cultured at 37°C, 250 rpm, for 18 hours to obtain seed culture. The seed culture was then inoculated into 50 ml of CM9 fermentation medium at a 1% (v / v) inoculum and cultured at 37°C, 250 rpm, for 24 hours. The fermentation product was harvested and centrifuged at 10,000 g for 2 minutes, and the supernatant was collected.

[0053] The supernatant was analyzed by HPLC for 1,3-dihydroxyacetone production. 1,3-dihydroxyacetone standard was purchased from Shanghai Myril Biochemical Technology Co., Ltd., catalog number B66080-500G. The elution time of 1,3-dihydroxyacetone was 9.5 min. The standard curve equation for HPLC quantitative detection of 1,3-dihydroxyacetone was: y = 346656x + 12665, R 2 = 0 9999. The supernatant was diluted a certain number of times and then subjected to HPLC quantitative detection. The peak area obtained was the y value. This was substituted into the 1,3-dihydroxyacetone standard curve equation to obtain the x value. After multiplying by the dilution multiple, the obtained value was the yield.

[0054] The calculation method of mass conversion rate is: The calculation method of molar conversion rate is: conversion rate (mol / mol) = (dihydroxyacetone production / 90) / ((total glucose content - residual sugar content) / 180)*100%

[0055] HPLC detection conditions: using Yuexu The fermentation products were analyzed by HPLC using a Sugar-Ca column. The column temperature was controlled at 70°C, the mobile phase was a 0.5 g / L EDTA-Ca aqueous solution, the flow rate was 0.4 ml / min, the injection volume of each sample was 20 μl, and the detection time was 30 minutes.

[0056] The shake flask fermentation test results are shown in Table 2 below. The 1,3-dihydroxyacetone yields of TZ-237 and TZ-249 were 12.99 g / L and 13.48 g / L, respectively. The yield of TZ-249 increased by 3.77%, and its glucose to 1,3-dihydroxyacetone conversion rate increased slightly to 0.96 mol / mol (Table 2).

[0057] Table 2 shows the shake flask fermentation of 1,3-dihydroxyacetone produced by recombinant Escherichia coli TZ-237 and TZ-249.

[0058] strains 1,3-Dihydroxyacetone (g / L) Conversion rate (mol / mol) TZ-237 12.99±0.06 0.95±0.009 TZ-249 13.48±0.14 0.96±0.009

[0059] Example 3: Fermentation of recombinant Escherichia coli TZ-237 in a 5 L fermenter

[0060] Seed culture medium: same as in Example 1.

[0061] CM9 fermentation medium: same as in Example 1.

[0062] Fermentation: A single recombinant E. coli TZ-237 clone grown on an LB plate was inoculated into 2 mL of seed culture medium and incubated at 37°C, 250 rpm, for 18 hours to obtain a seed culture. The next day, 2 mL of the seed culture was transferred to a 1-L Erlenmeyer flask containing 100 mL of fresh LB medium and incubated in a shaker at 37°C, 250 rpm. Once the seed culture reached an OD600 of 2-3, it was added to a 5-L fermentor containing 2 L of CM9 fermentation medium for fermentation. During the fermentation process, 700 g / L glucose was used as a feed, and ammonia was used as a neutralizer. Fermentation conditions were a starting OD600 nm of 0.1, a temperature of 37°C, a pH of 7.0, 30% dissolved oxygen, an upper limit of 10 L / min and a lower limit of 3 L / min, and an upper limit of 1000 rpm and a lower limit of 300 rpm, respectively. Dissolved oxygen levels were controlled post-inoculation using a cascade control system that included dissolved oxygen, agitation speed, and aeration.

[0063] The culture products obtained at different fermentation times were collected; they were centrifuged at 10,000 g for 2 min, and the supernatant was collected.

[0064] The supernatant was analyzed by HPLC to determine the yield of 1,3-dihydroxyacetone (ie, the DHA concentration in the fermentation supernatant, using the same method as in Example 2).

[0065] The calculation method of production rate is: production rate = 1,3-dihydroxyacetone concentration / fermentation time (g / L / h)

[0066] The recombinant E. coli TZ-237 was fermented in a 5L tank. The fermentation results were as follows: Figure 2 The results showed that after 36 h of fermentation, the yield of 1,3-dihydroxyacetone reached 40.6 g / L, the production rate reached 1.13 g / L / h, and the conversion rate of glucose to 1,3-dihydroxyacetone was 0.95 mol / mol (see Table 3).

[0067] Example 4: Fermentation of recombinant Escherichia coli TZ-249 in a 5 L fermenter

[0068] Seed culture medium: same as in Example 1.

[0069] CM9 fermentation medium: same as in Example 1.

[0070] Fermentation: A single recombinant E. coli TZ-249 clone grown on an LB plate was inoculated into 2 ml of seed culture medium and incubated at 37°C, 250 rpm, for 18 hours to obtain a seed culture. The next day, 2 ml of the seed culture was transferred to a 1-L Erlenmeyer flask containing 100 ml of fresh LB medium and incubated in a shaker at 37°C, 250 rpm. Once the seed culture reached an OD600 nm of 2-3, it was added to a 5-L fermentor containing 2 L of CM9 fermentation medium for fermentation. During the fermentation process, 700 g / L glucose was used as a feed, and ammonia was used as a neutralizer. Fermentation conditions were a starting OD600 nm of 0.1, a temperature of 37°C, a pH of 7.0, 30% dissolved oxygen, an upper limit of 10 L / min and a lower limit of 3 L / min, and an upper limit of 1000 rpm and a lower limit of 300 rpm, respectively. Dissolved oxygen levels were controlled post-inoculation using a cascade control system that included dissolved oxygen, agitation speed, and aeration.

[0071] The culture products obtained at different fermentation times were collected; they were centrifuged at 10,000 g for 2 min, and the supernatant was collected.

[0072] The supernatant was analyzed by HPLC to determine the yield of 1,3-dihydroxyacetone (using the same method as in Example 2).

[0073] The calculation method of production rate is: production rate = 1,3-dihydroxyacetone concentration / fermentation time (g / L / h)

[0074] The recombinant E. coli TZ-249 was fermented in 5L, and the fermentation results were as follows Figure 3 The results showed that after 36 h of fermentation, the yield of 1,3-dihydroxyacetone reached 103.1 g / L, the production rate reached 2.86 g / L / h, and the conversion rate of glucose to 1,3-dihydroxyacetone was 0.99 mol / mol (see Table 3).

[0075] Table 3 shows the 5L fermentation results of recombinant E. coli TZ-237 and TZ-249 for 1,3-dihydroxyacetone production.

[0076]

[0077] Based on the results shown in Examples 2-4 above, it can be seen that the recombinant E. coli TZ-249, derived from recombinant E. coli TZ-237 through evolutionary metabolism, has significantly improved its 1,3-dihydroxyacetone production capacity and tolerance to 1,3-dihydroxyacetone. This is undoubtedly of great significance for large-scale industrial production of 1,3-dihydroxyacetone.

[0078] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be practiced over a wide range of parameters, concentrations, and conditions without departing from the spirit and scope of the present invention and without unnecessary experimentation. Although specific embodiments have been given herein, it should be understood that further modifications may be made to the present invention. In summary, this application is intended to encompass any variations, uses, or improvements to the present invention, including those made by conventional techniques known in the art that depart from the scope of the present invention. Applications of the essential features may be made within the scope of the following claims.

Claims

1. Escherichia coli ( Escherichia coli ) TZ-249, its deposit number is CGMCC No. 26177.

2. A bacterial agent, the active ingredient of which is the Escherichia coli according to claim 1 ( Escherichia coli ) TZ-249 or its bacterial liquid or its fermentation liquid.

3. Use of TZ-249 or its bacterial solution or fermentation broth according to claim 1 or the bacterial agent according to claim 2 in at least one of the following: 1) Production of 1, 3-dihydroxyacetone; 2) Increase the yield of 1, 3-dihydroxyacetone; 3) Increase the production rate of 1,3-dihydroxyacetone; 4) Promote the conversion of glucose into 1,3-dihydroxyacetone.

4. A method for producing 1,3-dihydroxyacetone, comprising the steps of: Escherichia coli ) TZ-249 was fermented to obtain 1, 3-dihydroxyacetone.

5. The method according to claim 4, characterized in that: The fermentation culture utilizes glucose as a carbon source to produce 1,3-dihydroxyacetone.

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