Alkaline dyeing process of alkali-resistant medium disperse dye

Abstract

The invention relates to the technical field of textile dyeing, and particularly discloses a disperse dye alkaline dyeing process of an alkali-resistant medium. The invention relates to an alkali medium resistant disperse dye alkaline dyeing process, which comprises: (1) fabric pretreatment: carrying out desizing treatment and water washing treatment on a fabric; the pretreated fabric is immersed in dye liquor, the bath ratio is 1: (15-25), the temperature ranges from 55 DEG C to 65 DEG C, the pressure ranges from 1.5 MPa to 2.5 MPa, the treatment time ranges from 3 h to 4 h, the dye liquor is prepared from alkali-resisting disperse dye, an antibacterial additive, a leveling agent, a pH adjusting agent and water, and the pH ranges from 9 to 10; (3) post-treatment: soaping and washing the dyed fabric with water, and drying; in addition, the preparation method has the advantage of improving the dyeing effect of the alkaline dyeing process.

Description

Technical Field

[0001] The present application relates to the technical field of textile dyeing, and more specifically, to an alkaline dyeing process of disperse dyes in an alkali-resistant medium. Background Art

[0002] With the development of industry and life, the demand for fabrics has become more and more extensive. The beauty and comfort of fabrics have become people's main concerns. Textiles are usually dyed to make the yarns of textiles have a variety of colors, and then compounded through cutting, sewing or hot pressing to produce a variety of textiles.

[0003] Traditional disperse dyes are prone to hydrolysis under alkaline conditions and have poor stability. They are usually used for dyeing under acidic and weakly acidic conditions. This process tends to produce more wastewater and cause greater environmental pollution. At the same time, a variety of oligomers are easily generated under acidic conditions. The use of disperse dyes with better alkali resistance has a simpler process and can reduce the oligomer content on the surface of dyed fabrics. However, existing alkali-resistant disperse dyes have some problems, such as uneven dyeing and low color fastness. Summary of the invention

[0004] In order to improve the dyeing effect of the alkaline dyeing process, the present application provides an alkaline dyeing process of disperse dyes in an alkali-resistant medium.

[0005] In a first aspect, the present application provides a disperse dye alkaline dyeing process in an alkali-resistant medium, which adopts the following technical solution: A disperse dye alkaline dyeing process for an alkali-resistant medium comprises the following steps: (1) Fabric pretreatment: desizing and washing the fabric; (2) Dyeing treatment: the pretreated fabric is immersed in a dye solution with a bath ratio of 1:(15-25), a temperature of 55-65°C, a pressure of 1.5-2.5 MPa, and a treatment time of 3-4 hours. The dye solution includes the following raw materials: alkali-resistant disperse dyes, antibacterial additives, leveling agents, pH regulators, and water, and the pH is 9-10; (3) Post-treatment: The dyed fabric is soaped, washed and dried.

[0006] By adopting the above technical scheme, the pH adjuster can adjust the pH of the dye solution to alkaline, the alkali-resistant disperse dye has good stability under alkaline conditions, is not easy to hydrolyze and decompose, can maintain the structure and color of the dye itself, so that the dyed fabric has good color fastness and gloss, the leveling agent can improve the dispersion uniformity of the alkali-resistant disperse dye, thereby making the dyeing of the fabric more uniform, the antibacterial additive makes the fabric after the dye solution treatment have an antibacterial effect, the dyed fabric has good color and antibacterial performance, and there is no need to adjust the pH multiple times, and the dyeing process is simpler.

[0007] Preferably, the leveling agent is β-cyclodextrin, and the content of β-cyclodextrin in the dye solution is 2.15-2.68 g / L.

[0008] By adopting the above technical solution, β-cyclodextrin has an internal hydrophobic and external hydrophilic structure. Adding β-cyclodextrin can encapsulate the alkali-resistant disperse dyes to form microcapsules, thereby improving the dispersibility and solubility of the alkali-resistant disperse dyes, thereby achieving uniform dyeing and improving the dyeing effect of the dye solution.

[0009] Preferably, in the dye liquor, the content of the antibacterial additive is 0.68-1.12 wt %, the antibacterial additive comprises plant essential oil microcapsules and lysozyme microspheres, the capsule wall of the plant essential oil microcapsules is β-cyclodextrin, and the capsule core of the plant essential oil microcapsules is composite plant essential oil.

[0010] By adopting the above technical scheme, the capsule wall of the plant essential oil microcapsule is β-cyclodextrin, so that the plant essential oil microcapsule has good compatibility with the leveling agent, the composite plant essential oil has good antibacterial properties, so that the plant essential oil microcapsule can slowly release antibacterial substances, and the lysozyme microspheres are loaded with lysozyme, which has a good killing effect on bacterial microorganisms, etc. The two are compounded to form an antibacterial additive with good antibacterial properties.

[0011] Preferably, in the antibacterial additive, the mass ratio of the plant essential oil microcapsules to the lysozyme microspheres is (1.25-1.48):(0.84-0.95).

[0012] By adopting the above technical scheme, the mass ratio of plant essential oil microcapsules and lysozyme microspheres is controlled, so that the inhibitory effect of plant essential oil microcapsules on bacteria and the killing effect of lysozyme microspheres on bacteria cooperate with each other, exert a synergistic effect, and improve the antibacterial performance of the antibacterial additive.

[0013] Preferably, the composite plant essential oil comprises oregano essential oil and wormwood essential oil in a mass ratio of (1.12-1.35):(1.05-1.11).

[0014] By adopting the above technical scheme, both oregano essential oil and wormwood essential oil have good antibacterial properties, and the combination of the two has a synergistic effect, which can improve the antibacterial properties of the composite plant essential oil, thereby improving the antibacterial properties of the dyed fabric.

[0015] Preferably, the preparation method of the plant essential oil microcapsules comprises the following steps: dissolving β-cyclodextrin in water to obtain a β-cyclodextrin solution, dissolving the composite plant essential oil in anhydrous ethanol to obtain a composite plant essential oil solution, dripping the β-cyclodextrin solution dropwise into the composite plant essential oil solution in a 45°C water bath while stirring, refrigerating at 5°C overnight, filtering, washing, and vacuum drying to obtain the plant essential oil microcapsules.

[0016] By adopting the above technical solution, a plant essential oil microcapsule with β-cyclodextrin as the capsule wall and the composite plant essential oil as the capsule core is prepared, which can slowly release the composite plant essential oil to the outside and play an antibacterial effect.

[0017] Preferably, the mass ratio of the β-cyclodextrin to the composite plant essential oil is (0.56-0.75):(1.06-1.15).

[0018] By adopting the above technical scheme and controlling the mass ratio of β-cyclodextrin to the composite plant essential oil, the embedding rate and morphology of the plant essential oil microcapsules can be effectively controlled to obtain plant essential oil microcapsules with appropriate shapes and sizes.

[0019] Preferably, the preparation method of the lysozyme microspheres comprises the following steps: dissolving chitosan in a 2wt% acetic acid solution to form a chitosan solution, mixing glycerol and an emulsifier, adding the chitosan solution and performing shear emulsification for 25-30 minutes, adding a 25% glutaraldehyde solution, filtering, and drying to obtain chitosan microspheres, dispersing chitosan in water, adding a lysozyme solution, shaking at a constant temperature for 2.5-3 hours, filtering, and drying to obtain lysozyme microspheres.

[0020] By adopting the above technical scheme, lysozyme microspheres use chitosan microspheres to load lysozyme. Under alkaline conditions, the surface of chitosan microspheres has a negative charge. β-cyclodextrin, which serves as a leveling agent and the wall of plant essential oil microcapsules, also has a negative charge under alkaline conditions, so that the leveling agent and the antibacterial additive repel each other, which is beneficial to the uniform dispersion of the two in the dye solution.

[0021] In summary, this application has the following beneficial effects: 1. In the present application, the pH regulator can adjust the pH of the dye solution to be alkaline. The alkali-resistant disperse dye has good stability under alkaline conditions, is not easily hydrolyzed and decomposed, and can maintain the structure and color of the dye itself, so that the dyed fabric has good color fastness and gloss. The leveling agent can improve the dispersion uniformity of the alkali-resistant disperse dye, thereby making the dyeing of the fabric more uniform. The antibacterial additive enables the fabric treated with the dye solution to have an antibacterial effect, and the dyed fabric has good color and antibacterial properties. At the same time, there is no need to adjust the pH multiple times, and the dyeing process is simpler.

[0022] 2. In the present application, β-cyclodextrin has a hydrophobic structure inside and a hydrophilic structure outside. Adding β-cyclodextrin can encapsulate the alkali-resistant disperse dyes to form microcapsules, thereby improving the dispersibility and solubility of the alkali-resistant disperse dyes, thereby achieving level dyeing and improving the dyeing effect of the dye solution.

[0023] 3. In the present application, chitosan microspheres are used to load lysozyme. Under alkaline conditions, the surface of chitosan microspheres has a negative charge. β-cyclodextrin, which serves as a leveling agent and the wall of plant essential oil microcapsules, also has a negative charge under alkaline conditions, so that the leveling agent and the antibacterial additive repel each other, which is beneficial to the uniform dispersion of the two in the dye solution. DETAILED DESCRIPTION

[0024] The present application is further described in detail below with reference to the embodiments.

[0025] Preparation Examples 1-6 of Plant Essential Oil Microcapsules Preparation Example 1 The preparation method of plant essential oil microcapsules comprises the following steps: dissolving 4g of beta-cyclodextrin in 50mL of water to obtain a beta-cyclodextrin solution, dissolving a composite plant essential oil in 30mL of anhydrous ethanol to obtain a composite plant essential oil solution, wherein the mass ratio of the beta-cyclodextrin to the composite plant essential oil is 0.56:1.06, dripping the beta-cyclodextrin solution dropwise into the composite plant essential oil solution while stirring in a water bath at 45°C, refrigerating at 5°C overnight, filtering, washing, and vacuum drying to obtain plant essential oil microcapsules, wherein the composite plant essential oil comprises oregano essential oil and wormwood essential oil in a mass ratio of 1.12:1.05.

[0026] Preparation Example 2 The preparation method of plant essential oil microcapsules comprises the following steps: dissolving 4g of beta-cyclodextrin in 50mL of water to obtain a beta-cyclodextrin solution, dissolving a composite plant essential oil in 30mL of anhydrous ethanol to obtain a composite plant essential oil solution, wherein the mass ratio of the beta-cyclodextrin to the composite plant essential oil is 0.75:1.15, dripping the beta-cyclodextrin solution dropwise into the composite plant essential oil solution while stirring in a water bath at 45°C, refrigerating at 5°C overnight, filtering, washing, and vacuum drying to obtain plant essential oil microcapsules, wherein the composite plant essential oil comprises oregano essential oil and wormwood essential oil in a mass ratio of 1.35:1.11.

[0027] Preparation Example 3 The difference between Preparation Example 3 and Preparation Example 1 is that in Preparation Example 3, the mass ratio of β-cyclodextrin to the composite plant essential oil is 0.56:0.78.

[0028] Preparation Example 4 The difference between Preparation Example 4 and Preparation Example 1 is that in Preparation Example 4, the mass ratio of β-cyclodextrin to the composite plant essential oil is 0.56:1.85.

[0029] Preparation Example 5 The difference between Preparation Example 5 and Preparation Example 1 is that in Preparation Example 5, the composite plant essential oil includes oregano essential oil and wormwood essential oil in a mass ratio of 1.12:0.63.

[0030] Preparation Example 6 The difference between Preparation Example 6 and Preparation Example 1 is that in Preparation Example 6, the composite plant essential oil includes oregano essential oil and wormwood essential oil in a mass ratio of 1.12:1.54.

[0031] Preparation Example 7-8 of Lysozyme Microspheres Preparation Example 7 The preparation method of lysozyme microspheres comprises the following steps: dissolving chitosan in a 2wt% acetic acid solution to form a chitosan solution, mixing 100mL of glycerol and 15mL of Span-80, adding 5g of the chitosan solution and performing shear emulsification for 25-30min, adding 10mL of a 25% glutaraldehyde solution, filtering, and drying to obtain chitosan microspheres, dispersing 2g of chitosan in water, adding 100mL of a 1.5g / L lysozyme solution, shaking at a constant temperature for 2.5-3h, filtering, and drying to obtain lysozyme microspheres.

[0032] Preparation Example 8 The preparation method of lysozyme microspheres comprises the following steps: dissolving chitosan in a 2wt% acetic acid solution to form a chitosan solution, mixing 100mL of glycerol and 15mL of Span-80, adding 5g of the chitosan solution and performing shear emulsification for 25-30min, adding 10mL of a 25% glutaraldehyde solution, filtering, and drying to obtain chitosan microspheres, dispersing 2g of chitosan in water, adding 100mL of a 1.5g / L lysozyme solution, shaking at a constant temperature for 2.5-3h, filtering, and drying to obtain lysozyme microspheres. Example

[0033] Example 1 A disperse dye alkaline dyeing process for an alkali-resistant medium comprises the following steps: (1) Fabric pretreatment: desizing and washing the fabric; (2) Dyeing treatment: The pretreated fabric is immersed in a dye solution with a bath ratio of 1:15, a temperature of 55°C, a pressure of 1.5 MPa, and a treatment time of 4 hours. The dye solution comprises the following raw materials: an alkali-resistant disperse dye, an antibacterial additive, a leveling agent, a pH adjusting agent, and the balance is water. The pH adjusting agent is sodium hydroxide, and the pH of the dye solution is adjusted to 10. The alkali-resistant disperse dye is disperse orange HA with a content of 2 wt%. The leveling agent is β-cyclodextrin with a content of 2.15 g / L. The antibacterial additive comprises plant essential oil microcapsules and lysozyme microspheres in a mass ratio of 1.25:0.84, and the content of the antibacterial additive is 0.68 wt%. The plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 1, and the lysozyme microspheres are the lysozyme microspheres prepared in Preparation Example 7. (3) Post-treatment: The dyed fabric is soaped, washed and dried.

[0034] Example 2 A disperse dye alkaline dyeing process for an alkali-resistant medium comprises the following steps: (1) Fabric pretreatment: desizing and washing the fabric; (2) Dyeing treatment: The pretreated fabric is immersed in a dye solution with a bath ratio of 1:25, a temperature of 65°C, a pressure of 2.5 MPa, and a treatment time of 3 h. The dye solution includes the following raw materials: an alkali-resistant disperse dye, an antibacterial additive, a leveling agent, a pH regulator, and the balance is water. The pH regulator is sodium hydroxide, and the pH of the dye solution is adjusted to 9. The alkali-resistant disperse dye is disperse orange HA with a content of 2 wt%. The leveling agent is β-cyclodextrin with a content of 2.68 g / L. The antibacterial additive includes plant essential oil microcapsules and lysozyme microspheres in a mass ratio of 1.48:0.95, and the content of the antibacterial additive is 1.12 wt%. The plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 2, and the lysozyme microspheres are the lysozyme microspheres prepared in Preparation Example 8. (3) Post-treatment: The dyed fabric is soaped, washed and dried.

[0035] Example 3 The difference between Example 3 and Example 1 is that in Example 3, the mass ratio of the plant essential oil microcapsules to the lysozyme microspheres is 1.25:0.45.

[0036] Example 4 The difference between Example 4 and Example 1 is that in Example 4, the mass ratio of the plant essential oil microcapsules to the lysozyme microspheres is 1.25:1.98.

[0037] Example 5 The difference between Example 5 and Example 1 is that in Example 5, the plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 3.

[0038] Example 6 The difference between Example 6 and Example 1 is that in Example 6, the plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 4.

[0039] Example 7 The difference between Example 7 and Example 1 is that in Example 7, the plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 5.

[0040] Example 8 The difference between Example 8 and Example 1 is that in Example 8, the plant essential oil microcapsules are the plant essential oil microcapsules prepared in Preparation Example 6.

[0041] Example 9 The difference between Example 9 and Example 1 is that in Example 9, the content of β-cyclodextrin in the dye solution is 1.13 g / L.

[0042] Example 10 The difference between Example 10 and Example 1 is that in Example 10, the content of β-cyclodextrin in the dye solution is 4.56 g / L.

[0043] Embodiment 11 The difference between Example 11 and Example 1 is that in Example 11, the content of the antibacterial additive is 0.12 wt %.

[0044] Example 12 The difference between Example 12 and Example 1 is that in Example 12, the content of the antibacterial additive is 2.34 wt %.

[0045] Comparative Example Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that in Comparative Example 1, no leveling agent is added.

[0046] Comparative Example 2 The difference between Comparative Example 2 and Example 1 is that in Comparative Example 2, no antibacterial additive is added.

[0047] Test methods The polyester fabric was dyed according to the method of Examples 1-12 and Comparative Examples 1-2, and the dyed polyester fabric was subjected to a washing color fastness test and an antibacterial test. The washing color fastness test was conducted in accordance with GB / T 3921-2008, and the antibacterial test was conducted in accordance with GB / T20944.3-2008. The test bacteria was Staphylococcus aureus, and the results are recorded in Table 1.

[0048] Table 1 Washing color fastness and antibacterial properties of dyed fabrics According to Table 1, Examples 1-2 and Comparative Examples 1-2, it can be seen that the dyed fabric obtained in Example 1-2 has good water washing color fastness and antibacterial properties, the alkali-resistant disperse dye has good stability under alkaline conditions, is not easy to hydrolyze, can maintain the color and gloss of the alkali-resistant disperse dye, so that the dyed fabric has a better effect, and the combination effect of the dye and the fabric is better. β-cyclodextrin has an internal hydrophobic and external hydrophilic structure, which can include and separate the alkali-resistant disperse dye, improve its dispersibility and solubility, and have a leveling effect, while β-cyclodextrin is negatively charged under alkaline conditions. As the plant essential oil microcapsules and lysozyme microspheres as antibacterial additives, the plant essential oil microcapsules use β- Cyclodextrin and lysozyme microspheres are chitosan, which are negatively charged under alkaline conditions and repel each other, which can promote the rapid dispersion of the three in the dye solution, which is beneficial to the dispersion and distribution of the antibacterial additive and the improvement of the leveling effect. No leveling agent is added in comparative example 1, so that the water washing color fastness of comparative example 1 is greatly reduced, the alkali-resistant disperse dye is unevenly distributed during the dyeing process, and the combination effect of the dye and the fabric is weakened, thereby reducing the water washing color fastness. No antibacterial additive is added in comparative example 2, the plant essential oil microcapsule and the lysozyme microsphere have good antibacterial and sterilization effects, and the antibacterial additive formed by the compounding makes the fabric have good antibacterial performance, so the antibacterial performance of comparative example 2 is reduced.

[0049] Compared with Example 1-2, the antibacterial performance of Example 3-4 decreases. Example 3-4 changes the mass ratio of plant essential oil microcapsules and lysozyme microspheres. The plant essential oil microcapsules can slowly release the composite plant essential oil, which has an inhibitory effect on the growth of bacteria and microorganisms, while the lysozyme microspheres are loaded with lysozyme, which has a killing effect on bacteria and microorganisms. The two cooperate with each other to exert a synergistic effect, thereby improving the antibacterial performance of the antibacterial additive. The antibacterial performance of Example 3-4 decreases, indicating that the synergistic effect of the two is related to their mass ratio. When the mass ratio changes, the synergistic effect decreases, thereby reducing the antibacterial performance.

[0050] Compared with Example 1-2, the antibacterial performance of Example 5-6 is reduced. During the preparation of the plant essential oil microcapsules used in Example 5-6, the mass ratio of β-cyclodextrin to the composite plant essential oil was changed. The mass ratio of β-cyclodextrin to the composite plant essential oil directly affects the embedding rate and morphology of the plant essential oil microcapsules. When the mass ratio changes, the embedding rate of the plant essential oil microcapsules decreases, the shape becomes irregular, the effect is reduced, and the antibacterial performance of the fabric is reduced.

[0051] Compared with Example 1-2, the antibacterial property of Example 7-8 decreases. When preparing the plant essential oil microcapsules used in Example 7-8, the mass ratio of oregano essential oil and wormwood essential oil in the composite plant essential oil was changed. Both oregano essential oil and wormwood essential oil have good antibacterial properties. After the mass ratio between the two was changed in Example 7-8, the antibacterial property of the composite plant essential oil was weakened, indicating that there is a synergistic effect between oregano essential oil and wormwood essential oil. The antibacterial property is better when compounded according to the mass ratio in this application.

[0052] Compared with Example 1-2, the water washing color fastness of Example 9-10 is reduced. Example 9-10 changes the addition amount of β-cyclodextrin as a leveling agent. When the addition amount of the leveling agent is too small, the leveling effect of the alkaline disperse dye is not good, thereby reducing the water washing color fastness. When the addition amount of the leveling agent is increased, β-cyclodextrin is prone to agglomeration, which is not conducive to fully exerting the leveling effect.

[0053] Compared with Examples 1-2, the antibacterial performance of Example 11 decreased, while the antibacterial performance of Example 12 did not change significantly. Examples 11-12 changed the amount of antibacterial additive added. When the amount of antibacterial additive added was reduced, the antibacterial component content of the dye solution decreased, thereby reducing the antibacterial performance of the dyed fabric. When the amount of antibacterial additive added was increased, the antibacterial effect did not show a significant improvement, indicating that the improvement of the antibacterial performance of the fabric by the antibacterial additive has reached saturation.

[0054] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A disperse dye alkaline dyeing process for an alkali-resistant medium, characterized in that: The following steps are involved: (1) Fabric pretreatment: desizing and washing the fabric; (2) Dyeing treatment: the pretreated fabric is immersed in a dye solution with a bath ratio of 1:(15-25), a temperature of 55-65°C, a pressure of 1.5-2.5 MPa, and a treatment time of 3-4 hours. The dye solution includes the following raw materials: alkali-resistant disperse dyes, antibacterial additives, leveling agents, pH regulators, and water, with a pH of 9-10; (3) Post-treatment: The dyed fabric is soaped, washed and dried.

2. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 1, characterized in that: The leveling agent is β-cyclodextrin, and the content of β-cyclodextrin in the dye solution is 2.15-2.68 g / L.

3. The alkaline dyeing process of disperse dyes in an alkali-resistant medium according to claim 1, characterized in that: In the dye solution, the content of the antibacterial additive is 0.68-1.12wt%, and the antibacterial additive includes plant essential oil microcapsules and lysozyme microspheres. The capsule wall of the plant essential oil microcapsule is β-cyclodextrin, and the capsule core of the plant essential oil microcapsule is composite plant essential oil.

4. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 3, characterized in that: In the antibacterial additive, the mass ratio of the plant essential oil microcapsules to the lysozyme microspheres is (1.25-1.48):(0.84-0.95).

5. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 3, characterized in that: The composite plant essential oil comprises oregano essential oil and wormwood essential oil in a mass ratio of (1.12-1.35):(1.05-1.11).

6. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 3, characterized in that: The preparation method of the plant essential oil microcapsule comprises the following steps: dissolving beta-cyclodextrin in water to obtain a beta-cyclodextrin solution, dissolving a composite plant essential oil in anhydrous ethanol to obtain a composite plant essential oil solution, dripping the beta-cyclodextrin solution dropwise into the composite plant essential oil solution under a 45°C water bath while stirring, refrigerating at 5°C overnight, filtering, washing, and vacuum drying to obtain the plant essential oil microcapsule.

7. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 6, characterized in that: The mass ratio of the beta-cyclodextrin to the composite plant essential oil is (0.56-0.75):(1.06-1.15).

8. The disperse dye alkaline dyeing process of an alkali-resistant medium according to claim 3, characterized in that: The preparation method of the lysozyme microspheres comprises the following steps: dissolving chitosan in a 2wt% acetic acid solution to form a chitosan solution, mixing glycerol and an emulsifier, adding the chitosan solution and performing shear emulsification for 25-30 minutes, adding a 25% glutaraldehyde solution, filtering, and drying to obtain chitosan microspheres, dispersing the chitosan in water, adding the lysozyme solution, shaking at a constant temperature for 2.5-3 hours, filtering, and drying to obtain the lysozyme microspheres.

Images