A copper hydrogen phosphate-loaded hydrogel with pH-responsive multi-enzyme activity and its preparation method and application

By loading copper phosphate nanosheets into sodium alginate hydrogel, the switching of different enzyme activities under acidic and neutral conditions was achieved, solving the problem of single enzyme activity in nanozyme therapy, effectively resisting bacteria and promoting bone repair, and alleviating diabetic periodontitis.

CN120037178BActive Publication Date: 2025-09-09WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202510506167.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-04-22
Publication Date
2025-09-09
Estimated Expiration
2045-04-22

AI Technical Summary

Technical Problem

The enzyme activity and function of existing nanozymes for treating diabetic periodontitis are single, making it difficult to effectively regulate ROS in the periodontitis microenvironment, leading to increased alveolar bone destruction.

Method used

A copper phosphate-loaded hydrogel with pH-responsive multi-type enzyme activity was designed. By loading copper phosphate nanosheets into an injectable sodium alginate hydrogel, the variable valence state of copper ions was utilized to exhibit peroxidase-like and catalase activities under acidic and neutral conditions, respectively, to catalyze the generation or scavenging of ROS.

Benefits of technology

Under acidic conditions, it catalyzes ROS to generate OH to fight bacteria, and under neutral conditions, it removes H2O2 to relieve oxidative stress, synergistically promotes bone regeneration, and improves the therapeutic effect of periodontitis.

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Abstract

A copper hydrogen phosphate-loaded hydrogel with pH-responsive multi-enzyme activity, as well as its preparation method and application, is disclosed. In a neutral microenvironment, the material primarily exhibits catalase-like activity, scavenging H2O2 and alleviating oxidative stress. In a weakly acidic microenvironment, it primarily exhibits peroxidase-like activity, catalyzing H2O2 to produce the more toxic ·OH antibacterial compound. Simultaneously, copper's inherent osteogenic properties synergistically enhance bone regeneration. The present invention evaluates the SA / CuHP hydrogel's biocompatibility, antioxidant properties, osteogenic ability under oxidative stress conditions, and antibacterial efficacy under weakly acidic conditions through in vitro experiments. A periodontitis model in type 2 diabetic rats is established to verify its in vivo antibacterial and osteogenic effects and biosafety, providing a new strategy for the treatment of diabetic periodontitis.
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