Animal experiment system for treating acute lung injury through ultrasonic-assisted drug delivery

Abstract

The invention discloses an animal experiment system for treating acute lung injury through ultrasonic-assisted drug delivery, which comprises a halter-shaped breathing mask, an air inlet system, an anesthesia system, an exhaust system, a drainage system, an acute lung injury treatment module and an ultrasonic monitoring and regulating system, the mask is composed of a profiling mask body, a water collector, an air inlet connector, an air exhaust connector, a water drainage connector and a fixed contraction band, a microorganism filtering layer is arranged in the water collector, and the air inlet connector, the air exhaust connector and the water drainage connector are all intelligent connectors. According to the invention, a method for enhancing drug delivery and absorption by utilizing an ultrasonic technology is combined with formononetin for treating acute lung injury to be applied to animal experiments, and ultrasonic waves are transmitted into an animal experiment target region by utilizing an ultrasonic transducer through a water environment where an animal is located, so that an effective ultrasonic-assisted drug delivery animal experiment system is developed; therefore, the medicine can accurately reach the lesion position to achieve the best treatment effect.

Description

Technical Field

[0001] The present invention relates to the field of ultrasonic-assisted drug delivery, and particularly to an animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury. Background Art

[0002] Formononetin is a natural flavonoid compound. Scholars at home and abroad have conducted in-depth research on its pharmacological activities and revealed its potential application values in aspects such as anti-inflammation, antioxidant, anti-tumor, and antibacterial. The chemical structure of formononetin is 5,7-dihydroxy-4'-methoxyflavone, and its molecular formula is C16H12O5. Its structure contains multiple active groups, such as hydroxyl and methoxy groups, which endow it with various biological activities. Formononetin is a light yellow crystalline powder, soluble in organic solvents such as methanol and ethanol, and slightly soluble in water. It has been found to have the following effects:

[0003] 1. Anti-inflammatory effect: Research shows that formononetin exerts an anti-inflammatory effect by inhibiting the nuclear factor-κB (NF-κB) signaling pathway and reducing the production of inflammatory mediators such as TNF-α and IL-6. Animal experiments have shown that formononetin can significantly reduce the inflammatory response in model animals and has potential clinical application value;

[0004] 2. Antioxidant effect: Formononetin has strong free radical scavenging ability, can effectively inhibit lipid peroxidation, and protect cells from oxidative stress damage. Its antioxidant mechanism mainly activates the Nrf2 / ARE signaling pathway and promotes the expression of antioxidant enzymes such as SOD and GSH-Px;

[0005] 3. Anti-tumor effect: Formononetin exerts an anti-tumor effect through multiple mechanisms such as inducing apoptosis of tumor cells, inhibiting the proliferation and angiogenesis of tumor cells. Research shows that formononetin can significantly inhibit the growth of various tumor cells, such as breast cancer, lung cancer, and colon cancer cells, and has low toxicity to normal cells;

[0006] 4. Antibacterial effect: Formononetin has an inhibitory effect on a variety of bacteria and fungi, and its mechanism involves disrupting the microbial cell membrane structure, inhibiting DNA replication, and protein synthesis. Research shows that formononetin has strong antibacterial activity against pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, and Candida albicans;

[0007] The pharmacokinetic study of formononetin is still in its initial stage. Existing studies have shown that the absorption, distribution, metabolism, and excretion processes of formononetin in the body are relatively complex, and its bioavailability is low. To improve its bioavailability, some studies have attempted to use new drug delivery systems such as nanotechnology and liposomes for improvement. In recent years, with the in-depth study of formononetin, it has been found that it shows significant potential in the treatment of acute lung injury; acute lung injury is a severe clinical syndrome characterized by acute onset, diffuse parenchymal lung lesions, and resulting acute respiratory failure; this disease has a high incidence and mortality, posing a serious threat to the life and health of patients.

[0008] As a natural compound with various pharmacological activities such as anti-inflammatory, antioxidant, and anti-fibrotic effects, the mechanism of action of formononetin in the treatment of acute lung injury has gradually attracted attention; studies have shown that formononetin can effectively improve the pathophysiological process of acute lung injury, thereby reducing lung tissue damage and promoting lung function recovery, through multiple pathways such as inhibiting inflammatory responses, reducing oxidative stress injury, and regulating the function of immune cells. However, although formononetin shows good prospects in the treatment of acute lung injury, its specific mechanism of action still needs to be further studied in depth. To apply formononetin to clinical treatment, effective drug delivery methods and drug delivery systems also need to be developed to ensure that the drug can accurately reach the lesion site and exert the best therapeutic effect.

[0009] In animal experimental studies, to evaluate the therapeutic effect of formononetin on acute lung injury, a stable and reliable life support system needs to be constructed to ensure that experimental animals can maintain normal vital signs during the experiment, and real-time monitoring and precise regulation of key physiological indicators such as the respiration, body temperature, and anesthesia depth of experimental animals can be achieved, thus providing strong support for the experimental study of formononetin in the treatment of acute lung injury.

[0010] Currently, in the field of animal underwater experiments, dead animals (or ex vivo tissues) are often used for experimental research, which cannot simulate the normal responses of live animals to the experiment, cannot monitor the effects of the experiment on the physiological characteristics of animals, and thus has a great impact on the accuracy of experimental results.

[0011] Ultrasonic transmission underwater has the advantages of high energy, short wavelength, strong anti-interference ability, etc., and is more suitable for signal transmission in the underwater environment compared to electromagnetic waves. However, the underwater environment is a brand-new and challenging living condition for animals, especially the breathing problem has become a major difficulty during the experiment. In order to ensure that animals can breathe normally and maintain their vital activities during underwater experiments, a special life support system needs to be designed. Traditional underwater breathing devices often have many deficiencies; for example, the seal of the face mask is not good, which easily leads to water infiltration and affects the breathing of animals; the microenvironment inside the face mask (such as temperature and humidity) cannot be effectively controlled, making animals feel uncomfortable when wearing it for a long time; the connection of the pipeline is complex and error-prone, increasing the operation difficulty and risk of the experiment;

[0012] In view of the above-mentioned shortcomings of the existing experimental research, the purpose of the present invention is to provide a life support system and an ultrasonic monitoring system for underwater experiments of animals treated with formononetin for acute lung injury, which can help experimenters understand the status of animals and changes in the experimental environment in real time, and make corresponding adjustments according to needs, so as to ensure the stability and safety of the experiment and improve the accuracy of parameter detection in animal experiments. Summary of the Invention

[0013] The purpose of the present invention is to solve the shortcomings existing in the prior art, and to propose an animal experiment system for ultrasonic-assisted drug administration in the treatment of acute lung injury.

[0014] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0015] An animal experiment system for ultrasonic-assisted drug administration in the treatment of acute lung injury, including a cage-shaped breathing mask, an air intake system, an anesthesia system, an exhaust system, a drainage system, an acute lung injury treatment module, and an ultrasonic monitoring and control system. The mask is composed of a contoured mask, a water collector, an air intake interface, an exhaust interface, a drainage interface, and a fixed shrinkage band. A microbial filtration layer is provided inside the water collector, and the air intake interface, the exhaust interface, and the drainage interface are all intelligent interfaces, which have the function of automatically identifying and matching connection devices.

[0016] Preferably: The cage-shaped breathing mask is made of biocompatible materials, and a microenvironment control system is provided inside the mask. The microenvironment control system includes a temperature sensor and a temperature adjustment device to adapt to the changes in the body temperature of animals under different experimental conditions.

[0017] Furthermore: The air intake system includes an air intake port connected to the atmosphere, a high-precision humidity sensor, and a humidity adjustment device. The humidity adjustment device automatically adjusts the intake humidity according to the data of the humidity sensor to maintain the optimal range for the comfortable breathing of animals.

[0018] Furthermore, the intake system further includes a solenoid valve, which supports micro-intake adjustment and is integrated with a remote control function to achieve precise control and real-time adjustment of the intake volume.

[0019] As a preferred solution of the present invention, the anesthesia system includes an intake port connected to an anesthesia machine, an anesthetic concentration monitoring device, and a closed-loop control system. The closed-loop control system monitors and automatically adjusts the anesthetic gas concentration in real time to ensure the smoothness and safety of the anesthesia process.

[0020] As a further solution of the present invention, the exhaust system includes an exhaust pump pipeline connected to the mask exhaust port and a gas component analysis device. The gas component analysis device monitors the gas components in the mask in real time and automatically alarms and starts the exhaust pump when the concentration of harmful gases is detected to exceed the standard.

[0021] As a still further solution of the present invention, the drainage system includes a drainage pump and a water collector connected to the mask drainage port. A high-precision water level sensor is provided inside the water collector to monitor the water level change in real time and automatically alarm when the water level approaches the dangerous threshold. And the drainage pump is built-in with an intelligent maintenance program, which can perform self-checks regularly and report maintenance requirements.

[0022] On the basis of the foregoing solutions, the monitoring and control system includes a central control unit and a remote monitoring platform. The central control unit integrates advanced functions such as fault diagnosis, data analysis, and historical records. The remote monitoring platform supports real-time video monitoring and can be accessed through multiple terminals.

[0023] On the basis of the foregoing solutions, the acute lung injury treatment module uses a drug atomization device connected to the mask intake port to atomize the treatment drug and send it into the animal respiratory system. The drug delivery system can adjust the drug delivery volume, delivery speed, and delivery time according to experimental needs, and add a physiological parameter monitoring module to monitor the physiological indicators of the animal's respiratory rate, heart rate, and blood pressure in real time, as well as the changes in pulmonary imaging.

[0024] On the basis of the foregoing solutions, its experimental method includes the following steps:

[0025] S1: Preheat and calibrate the experimental equipment, and check whether all pipelines are intact and leak-free;

[0026] S2: Prepare and maintain a suitable water temperature environment;

[0027] S3: Guide the animal to the tooling area and use a fixing device to stabilize the animal;

[0028] S4: Select a cage-shaped mask that matches the animal's facial features and cover the animal's mouth and nose, and check the mask tightness;

[0029] S5: Turn on and check the operating status of all pipelines and pumps;

[0030] S6: After checking that the equipment is operating normally under simulated experimental conditions, place the animal in the experimental water environment.

[0031] S7: Open the anesthesia pipeline and / or the air inlet pipeline according to the experimental requirements, and closely monitor the animal's reaction and changes in the experimental environment, and adjust the parameters to ensure the stability and safety of the experimental environment.

[0032] S8: Record the key parameters during the experiment, and use the remote monitoring platform to observe the working status of each system and the animal status in real time.

[0033] The beneficial effects of the present invention are as follows:

[0034] 1. An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, by adopting biocompatible materials and a microenvironment control system, ensures that the mask causes no irritation to the facial skin during long-term wearing and adapts to the changes in the animal's body temperature under different experimental conditions. The system has real-time monitoring and alarm functions to ensure the stability and safety of the experimental environment.

[0035] 2. An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, the air inlet system, anesthesia system and exhaust system are equipped with sensors and automatic adjustment devices to monitor in real time and precisely adjust the key parameters of gas components such as humidity, anesthesia concentration, oxygen and carbon dioxide, as well as the adjustment of different concentration gradients of drugs. This not only ensures the stability and safety of the experimental environment, but also provides the necessary oxygen support and respiratory management for the treatment of acute lung injury, and helps to reduce lung inflammation and injury.

[0036] 3. An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, adopts a modular design and a remote ultrasonic monitoring platform, which is convenient for system upgrade, maintenance and remote monitoring. Monitor the experimental environment and animal status through ultrasonic underwater conduction, and respond to abnormal situations in a timely manner to improve the experimental efficiency.

[0037] 4. An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, the system can accurately control the experimental environment and record the key parameters during the experiment, so it enhances the repeatability of the experiment, which is of great significance for studying the pathogenesis, treatment effect and drug screening of acute lung injury animal models.

[0038] 5. An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, uses a gentle method to fix the animal, ensures that the mask is sealed but not too tight, monitors the animal's physiological indicators in real time and adjusts the experimental parameters in a timely manner, etc., which helps to reduce the pain and discomfort of the animal. Brief Description of the Drawings

[0039] Figure 1 is a schematic diagram of the installation of the experimental equipment of an animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury proposed by the present invention;

[0040] Figure 2 It is a schematic structural diagram of a breathing mask of an animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury proposed by the present invention. Specific embodiments

[0041] The technical solutions of this patent will be further described in detail below in conjunction with specific embodiments.

[0042] The embodiments of this patent are described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the drawings are exemplary and are only used to explain this patent and should not be construed as a limitation to this patent.

[0043] Example 1:

[0044] An animal experiment system for ultrasonic-assisted drug delivery in the treatment of acute lung injury, comprising:

[0045] A cage-shaped breathing mask

[0046] It consists of a contoured mask, a water collector, an air inlet interface, an exhaust interface, a drainage interface and a fixed shrinkage band; the mask sealing ring fits tightly against the animal's skin to ensure that the seal does not leak water. The air inlet at the oral cavity position is connected to the air inlet pipeline, the top exhaust port is connected to the exhaust pipeline, and the bottom water collection cavity is connected to the drainage pipeline. A biocompatible material is introduced to ensure that the long-term wearing has no irritation to the facial skin. At the same time, a microenvironment control system inside the mask is added, such as temperature sensing and adjustment functions, to adapt to the changes in the animal's body temperature under different experimental conditions. A microbial filtration layer is added inside the water collector to prevent microorganisms in the water from entering the respiratory system. At the same time, a transparent material is used to facilitate the experimenter to directly observe the water collection situation. The air inlet, exhaust and drainage interfaces are upgraded to intelligent interfaces with automatic recognition and matching functions to ensure correct connection and reduce human operation errors. Intelligent pressure-sensing materials are used to monitor and adjust the tightness of the belt in real time to ensure both stability and comfort;

[0047] An air intake system

[0048] The air inlet is connected to the atmosphere to ensure the entry of fresh air. There is a valve in the middle of the pipeline. A high-precision humidity sensor is integrated inside the mask to monitor the humidity change generated by the animal's breathing in real time. The humidity adjustment device automatically adjusts the humidity according to the sensor data to maintain it within the optimal range for the animal's comfortable breathing, avoiding dryness or excessive wetness of the respiratory tract. The humidity adjustment system has an adaptive algorithm built-in to dynamically adjust the humidity according to parameters such as the animal's breathing frequency and depth;

[0049] The solenoid valve supports micro air intake regulation to ensure accurate control of the air intake volume. It uses a low-power electromagnetic coil to reduce energy consumption and extend the service life of the solenoid valve. The solenoid valve integrates a remote control function, allowing experimenters to adjust the air intake volume in real time through the monitoring and control system.

[0050] Anesthesia system

[0051] The air inlet is connected to the anesthesia machine, and there is a valve in the middle of the pipeline. The anesthetic concentration monitoring device is linked with the anesthesia machine to form a closed-loop control system to monitor and automatically adjust the anesthetic gas concentration in real time. The safety upper and lower limits of the set anesthetic concentration are set. When the concentration exceeds the range, the system will automatically alarm and adjust to ensure a stable and safe anesthesia process. Combined with physiological monitoring equipment, it monitors the animal's response to anesthesia in real time.

[0052] The anesthesia machine supports the selection of multiple anesthesia modes, such as continuous anesthesia, intermittent anesthesia, programmed anesthesia, etc. Experimenters can customize anesthesia parameters such as anesthesia depth and duration according to factors such as animal species, body size, and experimental purposes.

[0053] Exhaust system

[0054] The exhaust pump pipeline is connected to the mask exhaust port to ensure gas circulation. The gas composition analysis device adds the monitoring of gas types such as nitrogen and carbon monoxide, and monitors the gas composition in the mask in real time. When the concentration of harmful gases detected exceeds the standard, the system will automatically alarm and start the exhaust pump to ensure the safety of the animal.

[0055] The exhaust pump adopts advanced noise reduction technologies such as mufflers and sound insulation materials to further reduce noise interference, improve the exhaust efficiency, and ensure unobstructed gas circulation in the mask.

[0056] Drainage system

[0057] The drainage pump is connected to the mask drainage port. The leaked water from the mask flows into the water collector under the action of gravity. The drainage pump continuously operates to discharge the leaked water in the water collector. The experimental water needs to maintain a constant temperature of about 39°C to avoid causing hypothermia in animals. A high-precision water level sensor is integrated inside the water collector to monitor the water level change in real time. When the water level approaches the dangerous threshold, the system will automatically alarm to remind the experimenter to deal with it in time to avoid flooding accidents.

[0058] The drainage pump is built-in with an intelligent maintenance program to perform self-checks regularly and report maintenance requirements such as cleaning and replacing the filter. The status of the drainage pump can be remotely monitored, and experimenters can understand the working status of the drainage pump at any time.

[0059] Acute lung injury treatment module

[0060] A drug atomization device connected through a mask air inlet atomizes the therapeutic drug and sends it into the animal respiratory system. The drug delivery system can adjust the drug delivery volume, delivery speed, and delivery time according to experimental requirements to ensure the effectiveness and safety of the therapeutic drug;

[0061] Add a physiological parameter monitoring module to monitor physiological indicators such as animal respiratory rate, heart rate, blood pressure, etc., as well as changes in pulmonary imaging in real time, providing data support for the evaluation of the treatment effect of acute lung injury;

[0062] Monitoring and Regulation System

[0063] The central control unit integrates advanced functions such as fault diagnosis, data analysis, and historical records, improving the overall performance of the system. It adopts a modular design, facilitating system upgrade and maintenance;

[0064] The remote ultrasound monitoring platform adds a real-time video monitoring function. Experimental personnel can remotely observe the animal's status based on the ultrasound signal, respond to abnormal situations in a timely manner, and support access from multiple terminals such as PCs, mobile phones, and tablets, facilitating experimental personnel to monitor the experimental environment anytime and anywhere.

[0065] The intelligent regulation algorithm makes personalized adjustments according to factors such as the species, body size of the experimental animal, experimental conditions, and the treatment requirements of acute lung injury, achieving more precise environmental control. The algorithm has an adaptive learning ability and can automatically adjust the control strategy according to the animal's physiological response and environmental changes during the experiment to ensure the stability of the experimental environment;

[0066] The specific experimental process includes the following steps:

[0067] S1: Ensure that the experimental area is clean, and experimental equipment (such as water temperature controllers, exhaust pumps, drainage pumps, etc.) has been preheated to the set temperature and calibrated. Check whether all pipelines (intake pipelines, anesthesia pipelines, exhaust pipelines, drainage pipelines) are intact and leak-free;

[0068] S2: Prepare and maintain a suitable water temperature environment, ensure that the water temperature is constant within the range acceptable to the animal's physiology, and record the initial water temperature;

[0069] S3: Guide the animal to the tooling area to reduce its nervousness. Use a fixing device designed specifically for animals to ensure that the animal's limbs and neck are stable but not oppressed, reducing the sense of restraint on the animal. Pay close attention to the animal's reaction during the fixing process to ensure that the fixing method is safe and comfortable;

[0070] S4: Select a cage-shaped mask that matches the animal's facial features, ensure that the inside of the mask is clean and free of foreign objects, gently cover the animal's mouth and nose with the mask to ensure that the mask fits tightly against the animal's face, use an adjustable strap to bypass from under the animal's chin and tie it tightly to the back of the head, and at the same time check the mask's tightness to ensure no air leakage;

[0071] S5: Sequentially connect the intake pipeline, the anesthesia pipeline, the exhaust pipeline, and the drainage pipeline, ensure that all pipelines are correctly connected, start the exhaust pump and the drainage pump, check the running status of the pumps and the drainage effect, and slowly open the valves of each pipeline to adjust to appropriate flow rates and pressures;

[0072] S6: Before placing the animal into the experimental water environment, first simulate the experimental conditions to check the mask tightness and whether the exhaust and drainage pipelines are unobstructed. After confirming that all equipment is operating normally, then slowly place the animal into the experimental water environment to avoid scaring or discomforting the animal and prevent the inducing factors of acute lung injury;

[0073] S7: According to the experimental requirements, selectively open the anesthesia pipeline and the intake pipeline to ensure that at least one pipeline remains open during the experiment to maintain the animal's breathing. Closely monitor the animal's reactions, such as physiological indicators like respiratory rate and heart rate, as well as changes in the experimental water environment (such as water temperature and water quality). Adjust parameters such as the intake volume and anesthesia concentration according to the real-time monitoring data to ensure a stable and safe experimental environment;

[0074] S8: Use an experimental record form or electronic device to detail the key parameters during the experiment (such as water temperature, gas concentration, animal physiological indicators, etc.), and use the remote monitoring platform to observe the working status of each system and the animal's status in real time.

[0075] As described above, this is a preferred specific embodiment of the present invention. The protection scope of the present invention is not limited thereto. Any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope disclosed by the present invention in combination with the prior art or common knowledge, within the spirit and principles of the present invention, shall be covered by the protection scope of the present invention.

Claims

1. An animal experimental system for ultrasound-assisted drug administration to treat acute lung injury, comprising a cage-shaped breathing mask, an air intake system, an anesthesia system, an exhaust system, a drainage system, an acute lung injury treatment module and an ultrasound monitoring and control system, characterized in that: The mask consists of a contoured mask, a water collector, an air inlet interface, an exhaust interface, a drainage interface and a fixed shrinkage belt. A microbial filtration layer is provided inside the water collector, and the air inlet interface, the exhaust interface and the drainage interface are all intelligent interfaces with the function of automatically identifying and matching connected devices.

2. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 1, characterized in that: The cage-shaped breathing mask is made of biocompatible materials, and a microenvironment control system is arranged inside the mask. The microenvironment control system includes a temperature sensor and a temperature regulating device to adapt to changes in animal body temperature under different experimental conditions.

3. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 2, characterized in that: The air intake system includes an air intake port connected to the atmosphere, a high-precision humidity sensor and a humidity adjustment device. The humidity adjustment device automatically adjusts the intake humidity according to data from the humidity sensor to maintain an optimal range for animal breathing comfort.

4. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 3, characterized in that: The air intake system also includes a solenoid valve, which supports micro-intake adjustment and is integrated with a remote control function to achieve precise control and real-time adjustment of the intake volume.

5. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 4, characterized in that: The anesthesia system includes an air inlet connected to an anesthesia machine, an anesthesia concentration monitoring device and a closed-loop control system. The closed-loop control system monitors and automatically adjusts the concentration of anesthetic gas in real time to ensure a smooth and safe anesthesia process.

6. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 5, characterized in that: The exhaust system includes an exhaust pump pipeline connected to the mask exhaust port and a gas composition analysis device. The gas composition analysis device monitors the gas composition in the mask in real time, and automatically alarms and starts the exhaust pump when it detects that the concentration of harmful gases exceeds the standard.

7. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 6, characterized in that: The drainage system includes a drainage pump and a water collector connected to the mask drain outlet. A high-precision water level sensor is provided inside the water collector to monitor water level changes in real time and automatically alarm when the water level approaches a dangerous threshold. The drainage pump has a built-in intelligent maintenance program that can perform regular self-inspections and report maintenance needs.

8. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 7, characterized in that: The ultrasonic monitoring and control system includes a central control unit and a remote monitoring platform. The central control unit integrates advanced functions such as fault diagnosis, data analysis and historical records; the remote monitoring platform supports real-time video monitoring and can be accessed through a variety of terminals.

9. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 8, characterized in that: The acute lung injury treatment module uses a drug atomization device connected to the mask air inlet to atomize the therapeutic drugs and deliver them into the animal's respiratory system. The drug delivery system can adjust the drug delivery volume, delivery speed and delivery time according to experimental requirements, and add a physiological parameter monitoring module to monitor the animal's respiratory rate, heart rate, blood pressure physiological indicators, and lung imaging changes in real time.

10. The animal experimental system for ultrasound-assisted drug delivery for treating acute lung injury according to claim 9, characterized in that: Its experimental method, The following steps are involved: S1: Preheat and calibrate the experimental equipment, and check whether all pipelines are intact and leak-free; S2: Prepare and maintain suitable water temperature environment; S3: Guide the animal to the harness area and secure it using a restraining device; S4: Select a bridle-shaped mask that matches the animal's facial features and cover the animal's mouth and nose, and check the tightness of the mask; S5: Connect and check the operating status of all pipelines and pumps; S6: After checking the normal operation of the equipment under simulated experimental conditions, the animals were placed in the experimental water environment; S7: Open the anesthesia circuit and / or air intake circuit according to the experimental requirements, pay close attention to the animal's reaction and changes in the experimental environment, and adjust the parameters to ensure a stable and safe experimental environment; S8: Record key parameters during the experiment and use the remote ultrasound monitoring platform to observe the working status of each system and the status of the animals in real time.

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