Cultivation method and application of Mongolian oak summer truffle mycorrhizal seedlings

Abstract

The invention discloses a cultivation method and application of Mongolian oak summer truffle mycorrhizal seedlings. Belongs to the technical field of truffle cultivation. The symbiotic relationship between Mongolian oak and summer truffle is determined for the first time, summer truffle inoculation liquid, pseudomonas, bacillus subtilis, gibberellin, glucose and charcoal are adopted to prepare summer truffle inoculation liquid, the summer truffle inoculation liquid is inoculated into Mongolian oak seedling root systems in a specific growth stage, mycorrhizal seedlings are cultivated, and the yield of the Mongolian oak seedlings is increased. According to the method, the mycorrhiza synthesis time is greatly shortened, the infection rate and the survival rate of mycorrhiza are increased, and the cultivation effect is improved. And the symbiosis of the truffles also improves the growth index of the Mongolian oak, and provides technical support for mycorrhizal seedling culture of the truffles.

Description

Technical Field

[0001] The invention relates to the technical field of truffle mycorrhizal seedling cultivation, and more particularly to a cultivation method and application of Mongolian oak summer truffle mycorrhizal seedlings. Background Art

[0002] Truffles belong to the genus Tuber in the order Tubrales, the family Tuberaceae, and the genus Tuber. The main edible species are black truffles, summer truffles, and white truffles. Truffles have a unique aroma, taste, and nutritional value. Humans have been eating truffles for thousands of years. They are the best of wild mushrooms and are known as the three great treasures along with caviar and foie gras.

[0003] Tube aestivum Vitt. is a type of truffle. Morphological characteristics: The ascocarp is irregularly spherical, often with a depression at the base, 3-6 cm in diameter, and has 5-6 angular warts on the surface, the diameter of which can reach 0.5 cm. The interior (spore body) is pale, then cream or dirty yellow-gray, or some with red or purple marble patterns, with interconnected and branched veins. The ascus is rod-shaped at the beginning, and later becomes nearly spherical, with an inconspicuous ascostalk, and generally has 4 ascospores inside. The spores are elliptical, with reticular patterns on the surface, 25-35μm×25-26μm. When mature, the fruiting body of the summer truffle is rich in nutrients, has a special aromatic smell, and coexists with the host. It is mostly produced in northern Europe such as the United Kingdom, and under broad-leaved forests in North America. In recent years, it has been found in the mountainous forests of northern and southwestern my country. Semi-artificial cultivation is mostly used in Europe. The commercial truffles in the domestic market are basically derived from wild resources. Due to the special growth characteristics of truffles and incorrect digging methods, the natural mushroom ponds and their ecological environment have been severely damaged, and the truffle production has dropped sharply, which is far from meeting the market demand. Therefore, it is becoming increasingly important to develop artificial cultivation methods for summer truffles.

[0004] At present, the artificial cultivation procedure of truffles is: cultivating seedlings → inoculating truffle spores at the roots of seedlings to form infected seedlings → transplanting infected seedlings → root turning and trimming and other management → fruiting bodies begin to form in 5 to 7 years, and the fruiting bodies can continue to form for 20 to 30 years. There are also some artificial cultivation methods for summer truffle mycorrhizal seedlings in the prior art, such as using chestnut, Pinus armandii, and Pinus yunnanensis as hosts and summer truffle as parasite to cultivate summer truffle mycorrhizal seedlings, but the above methods have a long time for mycorrhiza to appear, a low infection rate, and a low survival rate of mycorrhizal seedlings, resulting in unsatisfactory cultivation results.

[0005] Therefore, how to provide a method for cultivating summer truffle mycorrhizal seedlings with better effects is a technical problem that those skilled in the art urgently need to solve. Summary of the invention

[0006] In view of this, the present invention provides a cultivation method and application of Mongolian oak summer truffle mycorrhizal seedlings, which shortens the time of mycorrhiza emergence, increases the infection rate and survival rate of mycorrhiza, and greatly improves the cultivation effect.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] A method for cultivating Mongolian oak summer truffle mycorrhizal seedlings comprises the following steps:

[0009] (1) Preparation of Truffle Sporozoite Suspension: Take mature fruiting bodies of Truffle, sterilize, crush, add sterile water, prepare Truffle Sporozoite Suspension, and store for later use;

[0010] (2) Preparation of summer truffle inoculum: The summer truffle spore suspension obtained in step (1) is mixed with Pseudomonas, Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 20-25 parts: 4-6 parts: 0.5-1 parts: 3-4 parts: 10-15 parts: 15-20 parts to prepare a summer truffle inoculum;

[0011] (3) Inoculation: Place sterile seedlings of Quercus mongolica in a truffle inoculation solution for root dipping; plant the seedlings with roots dipped in a cultivation medium, and pour the remaining inoculation solution into the cultivation medium;

[0012] (4) Cultivation after inoculation: After the seedlings of step (3) are planted, management is performed during the seedling stage to prevent the occurrence of diseases and insect pests. The seedlings are cultured for 3-3.5 months to obtain summer tuber mycorrhizal seedlings.

[0013] Preferably, in step (1), the fruiting bodies are immersed in 75% alcohol for disinfection for 3-5 seconds; the rotation speed of the crushing is 8000-19000 rpm, and the crushing time is 10-30 seconds; and the storage temperature is 0-4°C.

[0014] Preferably, the particle size of the Truffle spore suspension in step (2) is 0.2-0.4 mm; and the particle size of the biochar is 0.6-0.7 mm.

[0015] Preferably, the number of effective spores in the truffle inoculum in step (2) is ≥ 6×10 6 / mL; the pH of the summer truffle inoculation solution is 7.5-8.5.

[0016] Preferably, the number of fibrous roots of the sterile Mongolian oak seedlings in step (3) is 3-4, and the length of the fibrous roots is 5-6 cm; the inoculation amount of each seedling is 0.1-0.2 mL of summer truffle inoculation solution.

[0017] Preferably, the cultivation matrix in step (3) comprises the following components in parts by weight: 10-20 parts of peat soil, 5-8 parts of perlite and 10-15 parts of vermiculite; the peat soil, perlite and vermiculite are all sterilized at 121°C.

[0018] Preferably, the pH of the cultivation medium in step (3) is 7.5-8.5, and the moisture content is 25-30%.

[0019] Preferably, the culture temperature in step (4) is 20-25° C., the substrate humidity is 25-28%, and the lighting conditions are 2000-5000 Lux.

[0020] Another object of the present invention is to provide: application of the above-mentioned cultivation method in cultivating summer tuber mycorrhizal seedlings.

[0021] It can be seen from the above technical solution that compared with the prior art, the present invention has the following beneficial effects:

[0022] The invention selects Mongolian oak and summer truffle to form a symbiotic relationship, adopts pseudomonas, bacillus subtilis, gibberellin, glucose and biochar to prepare summer truffle inoculation liquid, and inoculates the liquid into the root system of Mongolian oak seedlings at a specific growth stage to cultivate mycorrhizal seedlings, which greatly shortens the time of mycorrhiza emergence, improves the infection rate and survival rate of mycorrhiza, and improves the cultivation effect. Moreover, the parasitism of truffles also improves the growth state of Mongolian oak, providing technical support for the cultivation of truffle mycorrhiza. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0024] Figure 1 For: the morphology of mycorrhiza;

[0025] Figure 2 For: Enlarged picture of mycorrhizal morphology. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example 1

[0028] A method for cultivating Mongolian oak truffle mycorrhizal seedlings comprises the following steps:

[0029] (1) Preparation of truffle sporozoite suspension: Take mature fruiting bodies of truffle in a room temperature, wash the surface, place in 75% alcohol for disinfection for 3-5 seconds, use filter paper to absorb the alcohol on the surface, use a sterile knife to scrape the fruiting body epidermis, cut into small cubes of about 1 cm×1 cm, and set aside; wipe the blender with 75% alcohol, rinse with sterile water, repeat 3 times, weigh 65g of the fruiting bodies cut into small cubes each time, put them into the prepared blender, 8000rpm, crush for 30 seconds, add aseptic technique, and prepare truffle spore suspension, the particle size of the fungus block is 0.2-0.4mm, and store at 0°C for later use;

[0030] (2) Preparation of truffle inoculum: The truffle spore suspension obtained in step (1) was mixed with Pseudomonas (a strain disclosed in the prior art, with a deposit number of CGMCC No. 5040), Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 22:5:1:3:12:15 to prepare a truffle inoculum. The number of effective spores in the truffle inoculum was ≥ 6×10 6 / mL; the pH of the summer truffle inoculation solution is 7.5; the particle size of the biochar is 0.6-0.7mm;

[0031] (3) Inoculation: Place sterile Mongolian oak seedlings with 3-4 fibrous roots and 5-6 cm in length in the summer truffle inoculation solution obtained in step (2) and dip the roots (soaking the stem base for 15 minutes);

[0032] Mix 15 parts of peat soil, 6 parts of perlite and 12 parts of vermiculite, sterilize at 121°C, and use lime or Ca(OH)2 to adjust the pH of the substrate to 7.5 and the moisture content to 25% as the cultivation substrate;

[0033] The prepared substrate is placed in a container with a bottom diameter of ≥8.5 cm, a diameter of ≥10 cm, and a height of ≥18 cm. The total amount of the substrate accounts for 1 / 2 of the container. The seedlings with roots are planted in the container and covered with soil. The soil layer is 2 cm away from the container mouth. The remaining truffle inoculum is poured into the cultivation substrate. The inoculum amount for each seedling is 0.1 mL truffle inoculum.

[0034] (4) Cultivation after inoculation: After the seedlings of step (3) are planted, seedling management is carried out. During the cultivation period, water should be irrigated to keep the substrate moist. The substrate humidity is controlled at 25%, the cultivation temperature is controlled at 20° C., and the light condition is 2000 Lux. The cultivation is carried out for 3-3.5 months to obtain summer tuber mycorrhizal seedlings. When the mycorrhizal seedlings first develop powdery mildew, the diseased plants are sprayed with 0.5 mol / L lime water or the diseased plants are removed.

[0035] Example 2

[0036] A method for cultivating Mongolian oak truffle mycorrhizal seedlings comprises the following steps:

[0037] (1) Preparation of spore suspension of summer truffle: take mature fruiting bodies of summer truffle in a room temperature, wash the surface, place in 75% alcohol for disinfection for 3-5 seconds, use filter paper to absorb the alcohol on the surface, use a sterile knife to scrape the epidermis of the fruiting bodies, cut into small cubes of about 1 cm×1 cm, and set aside; wipe the blender with 75% alcohol, rinse with sterile water, repeat 3 times, weigh 65g of the fruiting bodies cut into small cubes each time, put them into the prepared blender, 19000rpm, crush for 10 seconds, add sterile water, and prepare a spore suspension of summer truffle, the particle size of the fungus cubes is 0.2-0.4mm, and store at 2°C for later use;

[0038] (2) Preparation of truffle inoculum: The truffle spore suspension obtained in step (1) was mixed with Pseudomonas (deposit number: CGMCC No. 5040), Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 20:4:0.5:4:10:20 to prepare a truffle inoculum. The number of effective spores in the truffle inoculum was ≥ 6×10 6 / mL; the pH of the summer truffle inoculum was 8.0; the particle size of the biochar was 0.6-0.7mm;

[0039] (3) Inoculation: Place sterile Quercus mongolica seedlings with 3-4 fibrous roots and 5-6 cm in length in the truffle inoculation solution obtained in step (2) and dip the roots (soaking the stem base for 25 min);

[0040] Mix 10 parts of peat soil, 5 parts of perlite and 10 parts of vermiculite, sterilize at 121°C, and use lime or Ca(OH)2 to adjust the pH of the substrate to 8.0 and the moisture content to 30% as the cultivation substrate;

[0041] The prepared substrate is placed in a container with a bottom diameter of ≥8.5 cm, a diameter of ≥10 cm, and a height of ≥18 cm. The total amount of the substrate accounts for 2 / 3 of the container. The seedlings with roots are planted in the container and covered with soil. The soil layer is 3 cm away from the container mouth. The remaining truffle inoculum is poured into the cultivation substrate. The inoculum amount for each seedling is 0.2 mL of truffle inoculum.

[0042] (4) Cultivation after inoculation: After the seedlings of step (3) are planted, seedling management is carried out. During the cultivation period, water should be irrigated to keep the substrate moist. The substrate humidity is controlled at 28%, the cultivation temperature is controlled at 25° C., and the light condition is 5000 Lux. The cultivation is carried out for 3-3.5 months to obtain summer tuber mycorrhizal seedlings. When the mycorrhizal seedlings first develop powdery mildew, the diseased plants are sprayed with 0.5 mol / L lime water or the diseased plants are removed.

[0043] Example 3

[0044] A method for cultivating Mongolian oak truffle mycorrhizal seedlings comprises the following steps:

[0045] (1) Preparation of spore suspension of summer truffle: take mature fruiting bodies of summer truffle in a room temperature, wash the surface, place in 75% alcohol for disinfection for 3-5 seconds, use filter paper to absorb the alcohol on the surface, use a sterile knife to scrape the epidermis of the fruiting bodies, cut into small cubes of about 1 cm×1 cm, and set aside; wipe the blender with 75% alcohol, rinse with sterile water, repeat 3 times, weigh 65g of the fruiting bodies cut into small cubes each time, put them into the prepared blender, 15000rpm, crush for 20 seconds, add sterile water, and prepare a spore suspension of summer truffle, the particle size of the fungus cubes is 0.2-0.4mm, and store at 0°C for later use;

[0046] (2) Preparation of truffle inoculum: The truffle spore suspension obtained in step (1) was mixed with Pseudomonas (deposit number: CGMCC No. 5040), Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 25:6:0.75:3.5:15:17 to prepare truffle inoculum. The number of effective spores in the truffle inoculum was ≥ 6×10 6 / mL; the pH of the summer truffle inoculum was 8.5; the particle size of the biochar was 0.6-0.7mm;

[0047] (3) Inoculation: Place sterile Mongolian oak seedlings with 3-4 fibrous roots and 5-6 cm in length in the summer truffle inoculation solution obtained in step (2) and dip the roots (soaking the stem base for 20 minutes);

[0048] Mix 20 parts of peat soil, 8 parts of perlite and 15 parts of vermiculite, sterilize at 121°C, and use lime or Ca(OH)2 to adjust the pH of the substrate to 8.5 and the moisture content to 28% as the cultivation substrate;

[0049] The prepared substrate is placed in a container with a bottom diameter of ≥8.5 cm, a diameter of ≥10 cm, and a height of ≥18 cm, with the total amount of the substrate accounting for 2 / 3 of the container. The seedlings with roots soaked are planted in the container and covered with soil, with the soil layer 2 cm away from the container mouth. The remaining truffle inoculum is poured into the cultivation substrate, with the inoculum amount of 0.15 mL truffle inoculum for each seedling;

[0050] (4) Cultivation after inoculation: After the seedlings of step (3) are planted, seedling management is carried out. During the cultivation period, water should be irrigated to keep the substrate moist. The substrate humidity is controlled at 26%, the cultivation temperature is controlled at 25° C., and the light condition is 3000 Lux. The seedlings are cultivated for 3-3.5 months to obtain summer tuber mycorrhizal seedlings. When the mycorrhizal seedlings first develop powdery mildew, the diseased plants are sprayed with 0.5 mol / L lime water or the diseased plants are removed.

[0051] Comparative Example 1

[0052] It is basically the same as Example 3, except that:

[0053] (2) Preparation of truffle inoculum: The truffle spore suspension obtained in step (1) was mixed with Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 25:0.75:3.5:15:17 to prepare a truffle inoculum. The number of effective spores in the truffle inoculum was ≥6×10 6 / mL; the pH of the summer truffle inoculum was 7.5; the particle size of the biochar was 0.6-0.7mm.

[0054] Comparative Example 2

[0055] It is basically the same as Example 3, except that:

[0056] The symbiotic tree species are different, they are from the genus Quercus, Quercus suber.

[0057] Comparative Example 3

[0058] It is basically the same as Example 3, except that:

[0059] (3) Inoculation: Place two sterile Mongolian oak seedlings with fibrous roots of 4 cm in length in the truffle inoculation solution obtained in step (2) and dip their roots (soaking them above the stem base for 20 minutes).

[0060] Comparative Example 4

[0061] It is basically the same as Example 3, except that:

[0062] (3) Inoculation: Place sterile Mongolian oak seedlings with 6 fibrous roots and 8 cm in length in the summer truffle inoculation solution obtained in step (2) and dip their roots (soaking them above the stem base for 20 minutes).

[0063] In order to evaluate the cultivation of mycorrhizal seedlings of different groups of summer tuber, the infection of mycorrhiza was continuously sampled and tested. The specific sampling method is as follows:

[0064] Random inspection: Random inspection shall be carried out at irregular intervals during the seedling raising period. Visually inspect the roots of the seedlings with soil growing to the surface of the substrate. If mycorrhizae are present, use a 20x or higher handheld magnifying glass to observe if the developmental morphology of the mycorrhizae is normal and free of contamination. Return the seedlings to the container and continue cultivating.

[0065] Microscopic examination: Randomly select mycorrhizal seedlings from time to time, take mycorrhizal sections to examine the mycorrhizae under a microscope, and the sections should have the morphological structure of truffle mycorrhizae.

[0066] Molecular testing: Cut the mycorrhizae of qualified mycorrhizal seedlings for microscopic examination, conduct DNA testing and sequence comparison, and remove and discard contaminated and non-tuber mycorrhizae.

[0067] The specific rules for random inspection of seedlings are shown in Table 1, the results of random inspection are shown in Table 2, and the specific morphology of mycorrhiza is shown in Table Figure 1 and Figure 2 shown.

[0068] Table 1 Sampling quantity for seedling testing

[0069] Number of seedlings Number of tested strains 500～1000 50 1001～10000 100 10001～50000 250 50001～100000 350 100001～500000 500 500001 and above 750

[0070] Table 2 Mycorrhizal infection in different groups

[0071]

[0072] Result analysis: According to the content of Table 1, the formation time of the truffle mycorrhizal seedlings of Examples 1-3 of the present invention is short, and the infection rate and survival rate are relatively high, indicating that the cultivation method of the present invention has achieved a more significant effect.

[0073] Furthermore, by comparing Example 3 with Comparative Example 1, it can be seen that when Pseudomonas (deposit number CGMCC No.5040) is added to the summer truffle inoculation solution, the formation time of mycorrhiza can be significantly shortened from 50 days to about 24 days, and the infection rate can also be increased. The reason may be that Pseudomonas can secrete a variety of plant hormones, which promote the branching and growth of the root system, and can provide more infection sites for truffle hyphae. At the same time, Pseudomonas can improve the soil microenvironment and secrete some antibiotics or signal molecules, which can not only promote the symbiosis between truffle spores and plant roots, accelerate the mycorrhiza formation time, and increase the infection rate, but also promote the growth of symbiotic plants.

[0074] Through the control experiment of Example 3 and Comparative Example 2, it can be seen that the symbiosis between truffles and plants exists in a very delicate relationship. Different truffle species coexist with different tree species. Due to the different physiological characteristics and root structures of the tree species, the symbiotic affinity with the truffles will be different. In Comparative Example 2, another tree species in the genus Quercus, Quercus suber, was used as a symbiotic plant and inoculated with truffle inoculum. Its mycorrhizal growth condition was far inferior to that of summer truffle and Mongolian oak.

[0075] It can be seen from Example 3 and Comparative Examples 3 and 4 that different inoculation times also affect the overall inoculation effect. When the number of fibrous roots of Mongolian oak seedlings is 3-4 and the length of the fibrous roots is 5-6 cm, the seedlings have the strongest affinity with the inoculated truffle spores and the inoculation effect is best.

[0076] At the same time, in order to verify the effect of symbiosis on the growth of Mongolian oak during the symbiosis between truffle mycorrhiza and Mongolian oak, the changes in the growth status of Mongolian oak in different groups compared with the initial stage of symbiosis were measured after 2 months of symbiotic cultivation, and a blank control group without truffle inoculation was set up.

[0077] The experimental results are shown in Table 3:

[0078] Table 3 Growth status of Quercus mongolica in different groups

[0079]

[0080] Result analysis: From the data in Table 3, it can be seen that the cultivation method of this method can not only improve the infection effect of mycorrhiza, but also, due to the symbiotic relationship between mycorrhiza and tree species, it can improve the absorption capacity of Mongolian oak for key nutrients in the soil to a certain extent, promote the growth of trees, and achieve a mutually beneficial symbiotic effect.

[0081] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0082] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for cultivating mycorrhizal seedlings of Quercus mongolica, characterized in that: The steps include: (1) Preparation of Truffle spore suspension: Take mature fruiting bodies of Truffle spore, sterilize, crush, add sterile water, prepare Truffle spore suspension, and store for later use; (2) Preparation of summer truffle inoculum: The summer truffle spore suspension obtained in step (1) is mixed with Pseudomonas, Bacillus subtilis, gibberellin, glucose and biochar in a weight ratio of 20-25 parts: 4-6 parts: 0.5-1 parts: 3-4 parts: 10-15 parts: 15-20 parts to prepare a summer truffle inoculum; (3) Inoculation: Place sterile seedlings of Quercus mongolica in a truffle inoculation solution for root dipping; plant the seedlings with roots dipped in a cultivation medium, and pour the remaining inoculation solution into the cultivation medium; (4) Cultivation after inoculation: After the seedlings of step (3) are planted, management is performed during the seedling stage to prevent the occurrence of diseases and insect pests. The seedlings are cultured for 3-3.5 months to obtain summer tuber mycorrhizal seedlings.

2. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein In step (1), the fruiting bodies are immersed in 75% alcohol for disinfection for 3-5 seconds; the rotation speed of the crushing is 8000-19000 rpm, and the crushing time is 10-30 seconds; and the storage temperature is 0-4°C.

3. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein The particle size of the Truffle spore suspension in step (2) is 0.2-0.4 mm; and the particle size of the biochar is 0.6-0.7 mm.

4. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein The number of effective spores in the truffle inoculation solution in step (2) is ≥ 6 × 10 6 / mL; the pH of the summer truffle inoculation solution is 7.5-8.

5.

5. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein The number of fibrous roots of the sterile Mongolian oak seedlings in step (3) is 3-4, and the length of the fibrous roots is 5-6 cm; the inoculation amount of each seedling is 0.1-0.2 mL of summer truffle inoculation liquid.

6. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein The cultivation matrix in step (3) comprises the following components in parts by weight: 10-20 parts of peat soil, 5-8 parts of perlite and 10-15 parts of vermiculite; the peat soil, perlite and vermiculite are all sterilized at 121°C.

7. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein: The pH of the cultivation medium in step (3) is 7.5-8.5, and the moisture content is 25-30%.

8. The method for cultivating the mycorrhizal seedlings of Quercus mongolica according to claim 1, wherein: The culture temperature in step (4) is 20-25° C., the substrate humidity is 25-28%, and the lighting condition is 2000-5000 Lux.

9. Application of any one of the cultivation methods of claims 1 to 8 in cultivating summer tuber mycorrhizal seedlings.

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