A composition with oil control and acne removal, barrier repair effect and application thereof

Abstract

The application provides a composition with oil control and acne removal, barrier repair effects and application thereof, and belongs to the field of cosmetics.The composition is composed of Sabina vulgaris leaf extract, Hibiscus sabdariffa flower extract, paeonol and Cryptomeria fortunei bud extract.The Sabina vulgaris leaf extract has the effects of balancing oil secretion and shrinking pores;the Hibiscus sabdariffa flower extract is rich in organic acids and anthocyanins, has the effects of dissolving pore plugs, controlling oil, resisting inflammation, smoothing acne skin and has the effects of moisturizing and removing redness;the paeonol has natural antiseptic effects on gram-positive bacteria and part of gram-negative bacteria, has the effects of acne removal, sterilization, inflammation reduction and whitening;the Cryptomeria fortunei bud extract can promote skin tissue structure regeneration and strengthen skin barrier.The composition effectively regulates excessive secretion of sebaceous gland oil and acne skin specific proteins through the synergistic effect of various components, promotes the health of acne skin, simultaneously repairs the skin barrier and enhances the self-protection ability of the skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics, specifically relating to a composition with oil-controlling, acne-removing, and barrier-repairing effects, and its application. Background Technology

[0002] In recent years, with the continuous development of dermatology and cosmetic science, the application of natural extracts in skin care products has become increasingly widespread, aiming to achieve multiple effects such as oil control, acne treatment, and skin barrier repair. However, existing skin care technologies still have some shortcomings in these aspects. For example, while acid-based acne treatment and oil control products can accelerate epidermal metabolism and promote exfoliation, improper use of acidic ingredients may lead to problems such as thinning of the stratum corneum, dryness, flaking, and barrier damage. Similarly, although vitamin B6 has advantages such as antioxidant, anti-inflammatory, immune function regulation, sebum secretion regulation, skin barrier improvement, prevention of various skin inflammations, and acne treatment, its easy oxidation, poor stability, low skin absorption rate, and difficulty in use in oil-soluble formulations limit its practical application. Therefore, exploring gentler and more effective methods for oil control, acne treatment, and barrier repair, as well as developing new natural extracts or their combinations, is of significant practical importance for improving skin problems. Summary of the Invention

[0003] This invention provides a composition and its application that have the effects of oil control, acne removal, and barrier repair. It works by combining extracts of *Solanum nigrum* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cephalotaxus fortunei* buds to achieve the effects of oil control, acne removal, and barrier repair.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] In a first aspect, the present invention provides a composition having the effects of oil control, acne removal, and skin barrier repair, the composition comprising the following raw materials in the indicated mass ratio:

[0006] The mass ratio of the extracts of *Solanum tuberosum* leaves, roselle flowers, tau tannin, and *Cryptomeria japonica* buds was (0.3-9):(2.5-25):(1-10):(3-6).

[0007] Preferably, the composition comprises raw materials in the following mass ratio:

[0008] The mass ratio of the extracts of *Solanum nigrum* leaves, roselle flowers, tau tannin, and *Cephalotaxus fortunei* buds was (6-9):(5-25):(7-10):(5-6).

[0009] More preferably, the composition comprises the following raw materials in the following mass ratio:

[0010] The mass ratio of the extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau phenol, and *Cryptomeria japonica* buds was 6:5:7:5.

[0011] Secondly, the present invention provides the application of the composition described in the first aspect in the preparation of skin care products with oil-controlling, acne-removing, and barrier-repairing effects.

[0012] Preferably, the dosage form of the skin care product is any one of face cream, lotion, serum, or face mask.

[0013] Thirdly, the present invention provides a lotion with oil-controlling, acne-reducing, and barrier-repairing effects, the lotion comprising the following components by weight percentage:

[0014] The composition described in the first aspect: 5-8 wt%;

[0015] Glycerin: 3-8 wt%

[0016] Vegetable oil: 1-5 wt%

[0017] Propylene glycol: 2-5 wt%

[0018] Cetearyl alcohol: 3-6 wt%

[0019] Polysorbate-60: 1-3 wt%;

[0020] Carbomer: 0.1-0.5 wt%

[0021] Triethanolamine: 0.1-0.5 wt%

[0022] Phenoxyethanol: 0.3-1 wt%;

[0023] The remainder is deionized water.

[0024] Preferably, the vegetable oil is sunflower seed oil and / or jojoba oil.

[0025] Fourthly, the present invention provides a method for preparing the emulsion described in the third aspect, comprising the following steps:

[0026] S1: At 75-85℃, deionized water, carbomer, polysorbate-60, cetearyl alcohol, and vegetable oil are stirred and mixed evenly to obtain a mixture.

[0027] S2: Cool the mixture to room temperature, then add each ingredient in the composition, as well as glycerin, propylene glycol, triethanolamine, and phenoxyethanol in sequence, and stir to mix well to obtain the emulsion.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0029] The composition provided by this invention contains extracts of *Solanum nigrum* leaves, *Hibiscus rosa-sinensis* flowers, tau phenol, and *Cephalotaxus fortunei* buds, which have synergistic effects in controlling oil production, treating acne, and repairing the skin barrier. *Solanum nigrum* leaf extract reduces the number of pores and shrinks pores by inhibiting sebaceous gland cell differentiation and reducing sebum secretion. It also evens out skin tone, smooths skin texture, and helps improve overall skin condition. *Hibiscus rosa-sinensis* flower extract promotes the production of tissue protein D, exfoliates keratin plugs, inhibits sebum secretion at its source, reduces blackheads, and refines pores. It also inhibits inflammatory factors, reduces the rate of inflammatory chain reactions, combats inflammation, conditions acne-prone skin, and improves skin radiance. tau phenol effectively inhibits the growth of *Propionibacterium acnes* and *Staphylococcus aureus*, and regulates sebum secretion, reduces pore blockage, and reduces inflammatory pigmentation. The anti-inflammatory and antioxidant properties of *Cephalotaxus fortunei* buds indirectly promote skin barrier repair. These four ingredients work synergistically through different mechanisms to improve skin problems, providing a comprehensive care solution for oily, acne-prone, and barrier-damaged skin. Detailed Implementation

[0030] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terminology used in the embodiments is for describing specific implementation schemes and does not constitute a limitation on the scope of protection of the present invention.

[0031] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by weight in percentages and parts.

[0032] Some of the raw materials and their sources are as follows:

[0033] AffIPORE leaf extract: purchased from Guangzhou Nuoran Biotechnology Co., Ltd., product name 71700-AFFIPORE TM .

[0034] Roselle flower extract: purchased from Shanghai Boshuo Industrial Co., Ltd., trade name: Roselle extract.

[0035] Japanese cedar bud extract: purchased from Shanghai Puen Biochemical Technology Co., Ltd., trade name: Japanese cedar bud extract.

[0036] Peach phenol: purchased from Guangzhou Nengguan Chemical Co., Ltd., CAS: 511-15-9, concentration 1wt%, solvent is butanediol, trade name: peach phenol.

[0037] All other raw materials and reagents were purchased commercially.

[0038] A composition with oil-controlling, acne-reducing, and skin barrier repair effects, the raw materials and their mass ratios are as follows:

[0039] Composition 1: Composed of extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cephalotaxus fortunei* buds in a mass ratio of 6:5:7:5.

[0040] Composition 2: Composed of extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cephalotaxus fortunei* buds in a mass ratio of 0.3:2.5:1:6.

[0041] Composition 3: Composed of extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cephalotaxus fortunei* buds in a mass ratio of 9:25:10:3.

[0042] Composition ①: Composed of extracts of *Solanum tuberosum* leaves, extracts of *Hibiscus rosa-sinensis* flowers, and tau tannins in a mass ratio of 6:5:7.

[0043] Composition ②: Composed of extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, and *Cephalotaxus fortunei* buds in a mass ratio of 6:5:5.

[0044] Composition ③: Composed of roselle flower extract, tau tannin, and Chinese cedar bud extract in a mass ratio of 5:7:5.

[0045] Composition ④: Composed of extracts of *Sophora japonica* leaves, tau tannin, and *Cryptomeria japonica* buds in a mass ratio of 6:7:5.

[0046] Composition ⑤: Composed of extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cephalotaxus fortunei* buds in a mass ratio of 1:0.1:0.5:10.

[0047] A lotion with oil-controlling, acne-reducing, and skin barrier-repairing effects has the following components and their mass percentages, as shown in Table 1:

[0048] Table 1. Component composition (by weight) of lotions with oil-controlling, acne-reducing, and barrier-repairing effects.

[0049] Emulsion 1 Emulsion 2 Emulsion 3 Emulsion 4 Emulsion 5 Composition / wt% 7 5 8 7 7 Composition Number 1 1 1 2 3 Glycerin / wt% 6 6 6 3 8 Vegetable oil / wt% 4 4 4 5 1 Propylene glycol / wt% 4 4 4 2 5 Cetearyl alcohol / wt% 5 5 5 6 3 Polysorbate-60 / wt% 2 2 2 3 1 Carbomer / wt% 0.3 0.3 0.3 0.5 0.1 Triethanolamine / wt% 0.3 0.3 0.3 0.1 0.5 Phenoxyethanol / wt% 0.7 0.7 0.7 0.3 1 Deionized water / wt% Add to 100 Add to 100 Add to 100 Add to 100 Add to 100

[0050] Note: The vegetable oil mentioned is jojoba oil.

[0051] The preparation steps for the lotion with oil-controlling, acne-reducing, and barrier-repairing effects are as follows:

[0052] S1: At 80℃, deionized water, carbomer, polysorbate-60, cetearyl alcohol, and vegetable oil are stirred and mixed evenly to obtain a mixture.

[0053] S2: Cool the mixture to room temperature, then add the following extracts in sequence: senna leaf extract, hibiscus flower extract, tau phenol, cedar bud extract, glycerin, propylene glycol, triethanolamine, and phenoxyethanol. Stir and mix well to obtain the emulsion.

[0054] Safety test

[0055] Using the 2015 "Cosmetic Safety Technical Specifications" as a reference standard, the safety of lotions 1-5 was evaluated, and skin patch tests were conducted on 30 people aged 16-65.

[0056] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the back or flexor side of the forearm of the subject with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0057] Evaluation criteria:

[0058] Grade 0: Negative reaction;

[0059] Grade 1: Suspicious reaction, with only slight erythema;

[0060] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0061] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0062] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0063] Test results: All subjects had negative skin reactions.

[0064] The above test results show that the lotion provided by this invention, which has the effects of oil control, acne removal, and barrier repair, is gentle and non-irritating to the skin and is safe to use.

[0065] Efficacy test

[0066] Oil control efficacy evaluation test

[0067] Test samples: Compositions 1-3, ①-⑤, and blank group.

[0068] Test sample preparation: Use deionized water to prepare a 7wt% mixture of compositions 1-3 and ①-⑤. The blank group contains only deionized water. Prepare the solution fresh for each use.

[0069] Sample volume: 2.0 ± 0.1 mL / cm 2 .

[0070] Test method: Refer to T / ZHCA 002-2018 Test method for oil control efficacy of cosmetics.

[0071] Testing instrument: Sebumeter (skin fat meter).

[0072] Test time point: 4 hours.

[0073] Test parallels: 3.

[0074] Test results: expressed as mean ± standard deviation.

[0075] Calculation method: Skin oil inhibition rate = [(C4-C0)-(T4-T0) / (C4-C0)]×100%

[0076] Where C4 is the skin oil content of the blank group after 4 hours; C0 is the initial skin oil content of the blank group; T4 is the skin oil content of the sample group after 4 hours; and T0 is the initial skin oil content of the sample group.

[0077] Table 2 Evaluation Test of Oil Control Efficacy

[0078] experimental group Skin oil suppression rate / % Composition 1 53.33±0.74 Composition 2 49.96±1.27 Composition 3 52.28±0.52 Composition ① 32.53±0.81 Composition ② 22.29±1.04 Composition ③ 31.32±0.93 Composition ④ 37.15±0.74 Composition ⑤ 43.04±0.41

[0079] As can be seen from the results of compositions 1-3, the compositions provided by the present invention have a good oil control effect, among which composition 1 has the best oil control effect.

[0080] Comparing the results of composition 1 with those of compositions ①-④ in this invention, it can be seen that the oil-controlling effect of the compositions is worse when any one of the following is missing: *Solanum nigrum* leaf extract, *Hibiscus rosa-sinensis* flower extract, tau phenol, and *Cephalotaxus fortunei* bud extract. This may be because *Solanum nigrum* leaf extract can inhibit aminopeptidase activity, thereby inhibiting the differentiation of precursor sebaceous gland cells and the secretion of sebum by mature sebaceous gland cells, regulating the expression of sebum-related genes, and thus reducing sebum synthesis; while *Hibiscus rosa-sinensis* flower extract and tau phenol can regulate skin pH, inhibit the growth of bacteria on the skin surface, and reduce excessive sebum secretion caused by bacterial activity; the flavonoids, natural polyphenols, and amino acids in *Hibiscus rosa-sinensis* flower extract and *Cephalotaxus fortunei* bud extract can provide protection for the skin, reduce the stimulation of the skin by external environmental factors, and thus reduce excessive sebum secretion. The four components work synergistically to reduce sebum secretion through multiple pathways.

[0081] Comparing the results of composition 1 and composition 5, it can be seen that when the proportions of the extracts of *Cephalotaxus fortunei* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cryptomeria japonica* buds in this invention are within the range defined by this invention, the composition has the best oil-controlling effect.

[0082] Acne-removing efficacy evaluation test

[0083] Acne is a chronic inflammation of the hair follicle and sebaceous gland. Excessive lipid secretion and keratin plugs within the hair follicle provide a suitable growth environment and nutrient source for anaerobic, lipophilic Propionibacterium acnes, leading to its excessive proliferation and biofilm formation. Under these conditions, the drug resistance and pathogenicity of the microorganisms are significantly increased. Therefore, by detecting the inhibitory effect of cosmetics on the biofilm activity of Propionibacterium acnes, the efficacy of the cosmetic in treating acne can be evaluated. The lower the biofilm activity (dc) value, the better the acne-reducing effect.

[0084] Test sample preparation: Use sterile distilled water to prepare a mixture of compositions 1-3 and ①-⑤ with a concentration of 7wt%.

[0085] Test method: Refer to T / LNPC 018-2024 Test method for evaluating the acne-removing efficacy of cosmetic raw materials.

[0086] Biofilm construction: Propionibacterium acnes was inoculated onto an agar slant and cultured for 24 hours. Sterile physiological saline was then added to prepare a bacterial concentration of 1×10⁻⁶. 8 CFU / mL bacterial suspension. Then, 2% of the inoculum was added to RCM medium. The blank control group was not inoculated with Propionibacterium acnes, but only RCM medium was added. After mixing well, the mixture was inoculated into 96-well plates and anaerobically cultured at 37°C until biofilm formation.

[0087] Drug administration test:

[0088] The experiment included a blank control group, a model control group, and a test sample group. The test samples were diluted twice with RCM medium, the original medium in the 96-well plate was discarded, and 200 μL of the diluted test samples were added to each well. The model control group and the blank control group were added only with RCM medium, 200 μL per well, and the samples were cultured for 24 h.

[0089] Sample determination:

[0090] After culturing for 24 hours, the culture medium was discarded, and resazurin reagent was added. The mixture was incubated at 37°C for 1 hour, and the biofilm activity was then detected.

[0091] Methods for detecting biofilm viability:

[0092] Fluorescence intensity I was measured at an excitation wavelength of 560 nm and an emission wavelength of 590 nm to calculate the relative activity of the biomembrane.

[0093] Test parallels: 3.

[0094] Test results: expressed as mean ± standard deviation.

[0095] The method for calculating the relative activity (dc) of biofilms is: dc = [(I 试 -I 空 ) / (I 模 -I空 )]×100%

[0096] Where dc represents the relative activity of the biomembrane; I 试 To test the fluorescence intensity of the sample group; I 空 Fluorescence intensity of the blank control group; I 模 The fluorescence intensity represents the fluorescence intensity of the model control group.

[0097] Table 3. Evaluation of Acne-Clearing Efficacy Tests

[0098] experimental group dc / % Composition 1 <![CDATA[43.51±0.76 * ]]> Composition 2 <![CDATA[49.63±0.77 * ]]> Composition 3 <![CDATA[44.74±1.11 * ]]> Composition ① <![CDATA[52.29±0.68 * ]]> Composition ② <![CDATA[79.41±0.45 * ]]> Composition ③ <![CDATA[55.69±0.78 * <!-- 5 -->]]> Composition ④ <![CDATA[53.55±1.29 * ]]> Composition ⑤ <![CDATA[51.47±0.46 * ]]> Model control group 100

[0099] Note: "*" indicates comparison with the model control group, p < 0.05.

[0100] Comparison of the results of the composition and the model control group showed that the composition can effectively reduce the biofilm activity of Propionibacterium acnes.

[0101] Comparing the results of compositions 1-3, it can be seen that composition 1 has the best effect in reducing the biofilm activity of Propionibacterium acnes.

[0102] Comparing the results of composition 1 with those of compositions ①-④, it is evident that the acne-reducing effect of the compositions is diminished when any one of the following is missing: *Solanum lyratum* leaf extract, *Hibiscus rosa-sinensis* flower extract, tsaool, or *Cephalotaxus fortunei* bud extract. This may be because *Solanum lyratum* leaf extract and *Hibiscus rosa-sinensis* flower extract can inhibit bacterial adhesion and growth; tsaool can directly disrupt bacterial cell membranes and kill bacteria; and *Cephalotaxus fortunei* bud extract can enhance the antibacterial effect by inhibiting bacterial biofilm formation and reducing bacterial activity. Therefore, in this invention, *Solanum lyratum* leaf extract, *Hibiscus rosa-sinensis* flower extract, tsaool, and *Cephalotaxus fortunei* bud extract exhibit a synergistic effect.

[0103] Comparing the results of composition 1 and composition 5, it can be seen that when the proportions of the extracts of *Cephalotaxus fortunei* leaves, *Hibiscus rosa-sinensis* flowers, tau tannin, and *Cryptomeria japonica* buds in this invention are within the range defined by this invention, the composition has the best effect on reducing the biofilm activity of *Propionibacterium acnes*.

[0104] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A composition with oil-controlling, acne-reducing, and barrier-repairing effects, characterized in that, It is composed of extracts of *Solanum nigrum* leaves, extracts of hibiscus flowers, tau phenol, and extracts of *Cryptomeria japonica* buds; the mass ratio of the extracts of *Solanum nigrum* leaves, extracts of hibiscus flowers, tau phenol, and extracts of *Cryptomeria japonica* buds is (6-9):(5-25):(7-10):(3-6). The AffIPORE leaf extract was purchased from Guangzhou Nuoran Biotechnology Co., Ltd., trade name: 71700-AFFIPORE TM ; The roselle flower extract was purchased from Shanghai Boshuo Industrial Co., Ltd., under the trade name: Roselle extract. The Cryptomeria bud extract was purchased from Shanghai Puen Biochemical Technology Co., Ltd., trade name: Cryptomeria bud extract; The taurolol was purchased from Guangzhou Nengguan Chemical Co., Ltd., CAS: 511-15-9, concentration 1 wt%, solvent is butanediol, trade name: taurolol.

2. The composition according to claim 1, characterized in that, The mass ratio of the extracts of *Solanum tuberosum* leaves, *Hibiscus rosa-sinensis* flowers, tau phenol, and *Cryptomeria japonica* buds is 6:5:7:

5.

3. The use of the composition as described in claim 1 or 2 in the preparation of skin care products with oil-controlling, acne-removing, and barrier-repairing effects.

4. The application as described in claim 3, characterized in that, The skincare product can be any one of the following forms: face cream, lotion, serum, or face mask.

5. A lotion with oil-controlling, acne-reducing, and barrier-repairing effects, characterized in that, Includes the following components by mass percentage: The composition according to claim 1 or 2: 5-8 wt% Glycerin: 3-8 wt% Vegetable oil: 1-5 wt% Propylene glycol: 2-5 wt% Cetearyl alcohol: 3-6 wt% Polysorbate-60: 1-3 wt%; Carbomer: 0.1-0.5 wt% Triethanolamine: 0.1-0.5 wt% Phenoxyethanol: 0.3-1 wt%; The remainder is deionized water.

6. The emulsion as described in claim 5, characterized in that, The vegetable oil is sunflower seed oil and / or jojoba oil.

7. A method for preparing the emulsion as described in claim 5, characterized in that, Includes the following steps: S1: At 75-85℃, deionized water, carbomer, polysorbate-60, cetearyl alcohol, and vegetable oil are stirred and mixed evenly to obtain a mixture. S2: Cool the mixture to room temperature, then add each ingredient in the composition, as well as glycerin, propylene glycol, triethanolamine, and phenoxyethanol in sequence, and stir to mix well to obtain the emulsion.