Virus on-site rapid detection equipment and application thereof

Abstract

The invention provides virus on-site rapid detection equipment and application thereof, and solves the technical problems that the existing public place virus on-site automatic detection is long in submission time, the detection result is lagged, and the detection cannot be carried out at any time and the like. The virus field rapid detection equipment is also provided with a slit, a grating, a detection mechanism and a spectrograph; the detection mechanism is provided with a sample bottle for containing a detected liquid reagent; the light source outputs a stable light beam with a fixed wavelength, the stable light beam enters the grating after passing through the slit, and passes through the sample bottle and the detected liquid reagent to form a transmission light beam after being split by the grating, the transmission light beam enters the spectrograph to detect the illumination intensity, and the virus on-site rapid detection equipment is applied to detection of coronavirus. The method can be widely applied to the technical field of virus detection.

Description

Technical Field

[0001] This application relates to the technical field of virus detection, and particularly to a rapid on-site virus detection device and its application. Background Art

[0002] As is well known, the common symptoms of people infected with the coronavirus include respiratory symptoms, fever, cough, shortness of breath, and difficulty breathing. In more severe cases, the infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. The coronavirus is highly contagious. For example, the novel coronavirus has caused a global pandemic, bringing not only respiratory symptoms but also "long COVID" symptoms such as loss of taste and smell and long-term fatigue, seriously damaging the physical and mental health of patients and disrupting the normal order of society.

[0003] Virus nucleic acid detection provides an effective means for diagnosing patients. However, due to reasons such as the long detection process and the dependence on professional detection sites, it greatly limits the on-site automatic detection of viruses in public places such as airports and stations. Once faced with a situation where the number of infected people is large and increasing rapidly, it is difficult to meet the diagnostic needs of a large number of suspected infected people and quarantined observers, resulting in a delay in the quarantine time, delaying the travel of passengers, and even posing a risk of a wider range of infections. Summary of the Invention

[0004] The purpose of the present invention is to solve the above-mentioned technical deficiencies, and provide a rapid on-site virus detection device and its application to achieve rapid and automatic on-site virus detection, meet the needs of on-site detection, and improve the efficiency of virus detection.

[0005] To this end, the present invention provides a rapid on-site virus detection device. The rapid on-site virus detection device is provided with a light source, and also provided with a slit, a grating, a detection mechanism, and a spectrometer. The detection mechanism is provided with a sample bottle for containing the liquid reagent to be measured. The light source outputs a stable beam with a fixed wavelength. The stable beam enters the grating after passing through the slit, and after being dispersed by the grating, it passes through the sample bottle and the liquid reagent to be measured inside to form a transmitted beam, and the transmitted beam enters the spectrometer for detecting the light intensity.

[0006] Preferably, the width of the slit is adjustable, and the adjustment range is 0 - 5 mm.

[0007] Preferably, the detection mechanism is further provided with a platform base for placing the sample bottle.

[0008] Preferably, the detection mechanism is further provided with a stepping motor and a transmission device. The transmission device is provided with an annular rack, an annular bracket, a support arm, and a gear. The annular rack is coaxially sleeved inside the annular bracket. The teeth of the annular rack are arranged on the annular inner side of the annular rack. One end of the support arm is connected to the annular rack. The other end of the support arm faces the annular bracket and extends out. And the body of the support arm is slidably connected to the annular bracket. The power output shaft of the stepping motor is connected to the gear, and the gear meshes with the annular rack. The number of the support arms is set to be multiple, and the multiple support arms are arranged on the annular rack at intervals. A platform base is connected to each support arm.

[0009] Preferably, a slider is connected to the body of the support arm, and the annular bracket is provided with a circumferential slideway matching the slider.

[0010] Preferably, a constant temperature component is further provided on the outer side of the annular bracket. The constant temperature component is provided with a heat sink and a semiconductor refrigerating sheet connected to each other. The other end of the support arm faces the annular bracket and extends above the constant temperature component. The platform base is arranged above the heat sink.

[0011] Preferably, the detection mechanism is further provided with a pushing device. The pushing device is provided with a baffle and a leakage plate. The baffle is provided with a support part and a blocking and pushing part connected to each other. The leakage plate is provided with leakage holes, and the leakage holes are arranged on one side of the baffle. The support part is connected to the leakage plate, and the blocking and pushing part is arranged above the platform base and is used for pushing down the sample bottle that has completed the detection and then entering the leakage holes.

[0012] Preferably, the platform base is provided with a C-shaped notch for placing the sample bottle. The leakage holes are arranged at intervals on the opening side of the C-shaped notch. The blocking and pushing part is provided with a V-shaped opening, and the orientation of the V-shaped opening is the same as that of the C-shaped notch.

[0013] Preferably, the on-site rapid virus detection device is further provided with an intelligent control system, an infrared sensor, and a temperature sensor. The infrared sensor is arranged on one side of the platform base and is used for detecting whether a sample bottle is placed on the platform base. The temperature sensor is connected to the heat sink. The intelligent control system is provided with an intelligent control device, and the intelligent control device is connected to the infrared sensor, the temperature sensor, the stepping motor, and the semiconductor refrigerating sheet respectively through control lines.

[0014] Application of the on-site rapid virus detection device according to any one of the above in detecting coronavirus.

[0015] The beneficial effects of the present invention are as follows: The present invention provides a rapid on-site virus detection device. The device is provided with a light source. The stable light beam with a fixed wavelength generated by the light source passes through a slit to obtain a light beam with an appropriate intensity, and then passes through a grating to obtain a highly pure monochromatic light beam. The monochromatic light beam passes through a sample bottle and the liquid reagent to be measured inside it, generating a transmitted light beam. The transmitted light beam enters a spectrometer and can be converted into an intensity signal, and the spectrometer detects the intensity value of the transmitted light beam. The turbidity of the liquid reagent to be measured in the sample bottle is positively correlated with the number of viruses, and there is a correlation between the turbidity of the liquid reagent to be measured and the intensity value of the transmitted light beam detected by the spectrometer. That is, as the turbidity of the liquid reagent to be measured increases, the intensity of the transmitted light beam formed by the same-intensity light beam passing through the sample bottle and the liquid reagent to be measured inside it becomes lower. By detecting the intensity value of the transmitted light beam with a spectrometer, looking up the corresponding value on the virus quantity-light intensity value standard curve for the detected light beam intensity value, the virus quantity can be obtained, and the sample detection is completed. The present invention does not rely on the existing professional detection sites of the technology and can be installed in public places such as airports and stations for rapid on-site virus detection. For example, in the detection of coronaviruses, the detection can be carried out on-site, greatly shortening the detection process, so as to meet the diagnostic needs of a large number of suspected infected persons and quarantined observers. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for use in the embodiments or the description of the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0017] Figure 1 It is a schematic structural diagram of the front view of the present invention;

[0018] Figure 2 is Figure 1 a schematic structural diagram of the perspective view shown;

[0019] Figure 3 is Figure 2 a schematic structural diagram of the perspective view of another angle with a part of the outer shell removed shown;

[0020] Figure 4 is Figure 3 a schematic structural diagram of the enlarged view of part A shown;

[0021] Figure 5 is a schematic structural diagram of the sample bottle;

[0022] Figure 6 is a schematic structural diagram of the platform base;

[0023] Figure 7 isFigure 6 Schematic structural diagram of the A-A cross-sectional view shown

[0024] Figure 8 is Figure 6 Schematic structural diagram of the perspective view shown

[0025] Figure 9 Schematic structural diagram of another form of the platform base

[0026] Figure 10 Schematic structural diagram of the front view of the detection cover

[0027] Figure 11 is Figure 10 Schematic structural diagram of the bottom view shown

[0028] Figure 12 is Figure 11 Schematic structural diagram of the B-B cross-sectional view shown

[0029] Figure 13 Working principle diagram of the intelligent control system of the present invention

[0030] Markings in the figure: 1. Light source, 2. Slit, 3. Grating, 4. Spectrometer, 5. Sample bottle, 6. Platform base, 7. Stepper motor, 8. Ring rack, 9. Ring bracket, 10. Support arm, 11. Gear, 12. Slide block, 13. Circumferential slideway, 14. Heat sink, 15. Semiconductor refrigeration sheet, 16. Leak plate, 17. Support part, 18. Blocking and pushing part, 19. Leak hole, 20. C-shaped notch, 21. V-shaped opening, 22. Infrared sensor, 23. Temperature sensor, 24. Intelligent control device, 25. First installation blind hole, 26. Second installation blind hole, 27. Elastic piece, 28. Bottle body, 29. Bottle cap, 30. Rounded corner structure, 31. Waste sample box, 32. Detection cover, 33. Sample bottle channel, 34. Platform base channel, 35. Light beam through hole, 36. Outer shell, 37. Bottle placing window, 38. Bottle taking window, 39. Touch display screen, 40. Input module, 41. Display module, 42. Power module, 43. Sample placement area. Detailed implementation manners

[0031] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application clearer and more understandable, the present application will be further described in detail below in conjunction with embodiments and the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application. The methods used in the present invention are all conventional methods unless otherwise specified; the raw materials and devices used are all conventional commercially available products unless otherwise specified.

[0032] Consisting of Figure 4 、 Figure 5As shown in the figure, the present invention provides a rapid on-site virus detection device. The rapid on-site virus detection device is provided with a light source 1. The rapid on-site virus detection device is also provided with a slit 2, a grating 3, a detection mechanism, and a spectrometer 4. The detection mechanism is provided with a sample bottle 5 for containing the liquid reagent to be detected. The light source 1 outputs a stable beam with a fixed wavelength. After passing through the slit of the slit 2, the stable beam enters the grating 3. After being dispersed by the grating 3, it passes through the sample bottle 5 and the liquid reagent to be detected inside to form a transmitted beam. The transmitted beam enters the spectrometer 4 for detecting the light intensity.

[0033] The wavelength of the light source 1 is a fixed wavelength, and the wavelength range is preferably 400 - 1000 nm. The beam within this wavelength range can generate a more stable transmitted beam after passing through the sample bottle 5 and the liquid reagent to be detected inside. The light source 1 preferably adopts a tungsten halogen light source.

[0034] The purpose of the slit 2 is to reduce the intensity of the beam of the light source 1 so that the intensity of the transmitted beam is within the working range of the spectrometer 4. It is preferably an adjustable slit, and the adjustment range is 0 - 5 mm. For light sources 1 with different intensities, the same or corresponding beam intensity can be obtained by adjusting the size of the slit of the slit 2. The slit 2 preferably adopts a symmetric slit that can be adjusted on both sides.

[0035] The purpose of the grating 3 is to disperse the beam transmitted through the slit 2 to obtain a highly pure monochromatic beam. According to the Lambert-Beer law, the highly pure monochromatic beam can improve the detection accuracy of the spectrometer 4. The grating 3 preferably adopts a transmission grating.

[0036] The sample bottle 5 is used to contain the liquid reagent to be detected. The liquid reagent to be detected contains antibodies corresponding to the types of viruses to be detected. For example, when detecting the 2019-nCoV virus, the liquid reagent to be detected is the corresponding antibody of this virus, such as 5A6 and / or 8A13 antibodies. In order to make the beam more easily penetrate the bottle body 28 of the sample bottle 5 and minimize the influence on the beam intensity, the bottle body 28 of the sample bottle 5 is preferably made of a colorless and transparent material, such as colorless and transparent glass, etc.; the beam preferably penetrates vertically into the bottle body 28 of the sample bottle 5 and then penetrates vertically out of the bottle body 28. The liquid reagent to be detected contains viruses. In order to prevent the liquid reagent to be detected from polluting the external environment or being polluted by the external environment, after the liquid reagent to be detected is filled into the sample bottle 5, it is preferably sealed in time. According to the actual situation of virus detection, the volume of the liquid reagent to be detected is usually 200 - 700 μL. Therefore, the internal cavity volume of the sample bottle 5 is less than 1 mL.

[0037] The spectrometer 4 is used to detect the intensity of the transmitted light beam formed by passing through the sample bottle 5 and the liquid reagent to be measured inside it. By detecting the intensity of the transmitted light beam, the amount of virus contained in the sampling sample added into the sample bottle 5 is determined. When the virus is added into the liquid reagent to be measured, specific binding occurs, which affects the turbidity of the liquid reagent to be measured. Moreover, the turbidity of the liquid reagent to be measured is positively correlated with the number of viruses, that is, the more the number of viruses, the higher the turbidity of the liquid reagent to be measured; there is a correlation between the turbidity of the liquid reagent to be measured and the intensity value of the transmitted light beam detected by the spectrometer 4, that is, as the turbidity of the liquid reagent to be measured increases, the intensity of the transmitted light beam formed by the same-intensity light beam passing through the sample bottle 5 and the liquid reagent to be measured inside it becomes lower; by detecting the intensity value of the transmitted light beam with the spectrometer 4, looking up the corresponding value in the virus quantity-light intensity value standard curve for the detected light intensity value, the virus quantity can be obtained, and the sample detection is completed. The spectrometer 4 usually adopts an infrared spectrometer.

[0038] The method for making the virus quantity-light intensity value standard curve is as follows: equally divide the same liquid reagent to be measured into multiple identical sample bottles 5; add different determined amounts of virus into the liquid reagent to be measured in the sample bottles 5 respectively, mix evenly to prepare the liquid reagent to be measured containing virus standards with different concentrations, and then seal the sample bottles 5; use the device of the present invention to detect the above sample bottles 5 respectively. During the operation process, the difference is only that the concentrations of the virus standards in the liquid reagent to be measured in the sample bottles 5 are different, and the others are the same, and the corresponding light intensity values are obtained respectively, and they are fitted into the virus quantity-light intensity value standard curve.

[0039] During the actual detection operation process, for the detection of the light intensity value by the on-site rapid virus detection device, the difference is only that the concentration of the virus in the liquid reagent to be measured in the sample bottle 5 is different, and the others are the same as those of the virus standard in the liquid reagent to be measured in the sample bottle 5.

[0040] The working principle of the present invention is as follows: The present invention provides a rapid on-site virus detection device, which does not rely on the existing professional detection sites in the art and can be installed in public places such as airports and stations for rapid on-site virus detection. Detection can be carried out on-site, greatly shortening the detection process, so as to meet the diagnostic needs of a large number of suspected infected persons and quarantined observers. The rapid on-site virus detection device is provided with a light source 1. The stable light beam with a fixed wavelength generated by the light source 1 passes through a slit 2 to obtain a light beam with appropriate intensity, and then passes through a grating 3 to obtain a highly pure monochromatic light beam. The monochromatic light beam passes through a sample bottle 5 and the liquid reagent to be tested inside it to generate a transmitted light beam. The transmitted light beam enters a spectrometer 4 and can be converted into an intensity signal, and the spectrometer 4 detects the intensity value of the transmitted light beam. The turbidity of the liquid reagent to be tested in the sample bottle 5 is positively correlated with the number of viruses, and there is a correlation between the turbidity of the liquid reagent to be tested and the intensity value of the transmitted light beam detected by the spectrometer 4, that is, as the turbidity of the liquid reagent to be tested increases, the intensity of the transmitted light beam formed by the same-intensity light beam passing through the sample bottle 5 and the liquid reagent to be tested inside it becomes lower; by detecting the intensity value of the transmitted light beam by the spectrometer 4, looking up the corresponding value in the virus quantity-light intensity value standard curve for the detected light beam intensity value, the virus quantity can be obtained, and the virus detection of the liquid reagent to be tested is completed.

[0041] In some embodiments, the detection mechanism of the present invention preferably further includes a platform base 6 for placing and fixing the sample bottle 5 to ensure the stable and reliable state of the sample bottle 5 during the detection process. The platform base 6 can be an existing device or Figure 4 、 Figures 6 - 8 the platform base 6 shown. For the convenience of installation and removal of the sample bottle 5, the platform base 6 is provided with a C-shaped notch 20 for placing the sample bottle 5. When placing the sample bottle 5, the bottom of the sample bottle 5 is inserted horizontally from the opening of the C-shaped notch 20, so that the bottom of the sample bottle 5 is clamped inside the C-shaped notch 20, thus completing the placement and fixation of the sample bottle 5. As a further preference, a first installation blind hole 25 adapted to the bottom of the sample bottle 5 is provided inside the C-shaped notch 20. The bottom wall from the bottom wall of the first installation blind hole 25 to the bottom wall of the opening of the C-shaped notch 20 shows a slow upward trend, which is convenient for the bottom of the sample bottle 5 to enter the inside of the C-shaped notch 20 horizontally from the opening of the C-shaped notch 20 and then be inserted into the first installation blind hole 25, making the placement of the sample bottle 5 more fixed and reliable. After the virus detection is completed, the sample bottle 5 is removed by an operation opposite to the installation sequence, which is convenient and fast. In addition, the platform base 6 can also be Figure 9The platform base 6 shown has a second installation blind hole 26. A resilient piece 27 is connected to the side wall of the second installation blind hole 26. The number of resilient pieces 27 is multiple, and they are evenly distributed on the side wall of the second installation blind hole 26 to form a biting structure with a hole in the middle. When installing the sample bottle 5, the bottom of the sample bottle 5 is inserted into the second installation blind hole 26 from top to bottom. The resilient pieces 27 forming the biting structure are deformed and clamped on the outer wall of the bottom of the sample bottle 5, completing the installation of the sample bottle 5. After the virus detection is completed, the sample bottle 5 is removed by an operation opposite to the installation sequence, and the resilient pieces 27 forming the biting structure return to their original state, which is convenient and fast.

[0042] The sample bottle 5 can be a commercially available product or can be the sample bottle 5 as shown in Figure 5 Figure 5. The bottle body 28 of the sample bottle 5 is a cylindrical structure. The upper part of the bottle body 28 is provided with a bottle mouth, and the bottle mouth is threadedly and sealingly connected to the bottle cap 29. Rounded corner structures 30 are provided around the bottom of the bottle body 28; a cylindrical cavity communicating with the bottle mouth is provided inside the sample bottle 5, and the cylindrical cavity is coaxially arranged with the bottle body 28 of the sample bottle 5.

[0043] In some embodiments, as shown in Figure 3 and Figure 4 Figure 12, the detection mechanism preferably further includes a stepper motor 7 and a transmission device. The transmission device includes an annular rack 8, an annular bracket 9, a support arm 10, and a gear 11; the annular rack 8 is coaxially sleeved inside the annular bracket 9, and the teeth of the annular rack 8 are arranged on the annular inner side of the annular rack 8. One end of the support arm 10 is connected to the annular rack 8, and the other end of the support arm 10 faces the annular bracket 9 and extends, and the arm body of the support arm 10 is slidably connected to the annular bracket 9; the power output shaft of the stepper motor 7 is connected to the gear 11, and the gear 11 meshes with the teeth on the annular inner side of the annular rack 8; the number of support arms 10 is set to be multiple, and the multiple support arms 10 are spaced apart on the annular rack 8, preferably distributed in a circular array centered on the central axis of the annular rack 8. Each support arm 10 is connected with a platform base 6. Under the power output of the stepper motor 7, through the transmission of the gear 11, the annular rack 8 is driven to rotate self - axially, thereby driving the support arm 10 together with the platform base 6 on the support arm 10 to rotate around the central axis of the annular rack 8. When the virus detection of the sample bottle 5 on one platform base 6 is completed, through the drive of the stepper motor 7, the platform base 6 together with the sample bottle 5 is moved out of the detection point. At the same time, the next platform base 6 together with the sample bottle 5 to be tested for the virus is moved into the detection point, realizing automatic sample injection and sampling, which is more convenient and fast.

[0044] In some embodiments, as shown in Figure 4 Figure 17, in order to make the drive of the annular rack 8 more stable and reliable during the self - axial rotation process, a slider 12 is preferably connected to the arm body of the support arm 10, and the annular bracket 9 is provided with a circumferential slideway 13 matching the slider 12.

[0045] In some embodiments, as Figure 3 , Figure 4 shown, in order to further ensure that the liquid reagent to be tested in the sample bottle 5 is in a constant temperature state and make the virus detection result more accurate, a constant temperature component is preferably provided on the outer side of the annular bracket 9. The constant temperature component is provided with a heat sink 14 and a semiconductor refrigeration sheet 15 which are connected to each other. The other end of the support arm 10 faces the annular bracket 9 and extends above the constant temperature component. The platform base 6 is arranged above the heat sink 14. The heat sink 14 provides heat for the liquid reagent to be tested in the sample bottle 5 installed on the platform base 6. The present invention uses the semiconductor refrigeration sheet 15 to perform constant temperature regulation on the temperature of the heat sink 14. The semiconductor refrigeration sheet 15 has two sides. One side has an endothermic function. After absorbing heat, the temperature decreases, realizing a cooling effect. One side has a heat dissipation function, realizing a heating effect.

[0046] In some embodiments, as Figure 4 shown, in order to reduce labor costs and further improve the mechanization level of the present invention, the detection mechanism is preferably further provided with a pushing device. The pushing device is provided with a baffle and a leakage plate 16. The baffle is provided with a support part 17 and a blocking and pushing part 18 which are connected to each other. The leakage plate 16 is provided with leakage holes 19. The leakage holes 19 are arranged on one side of the baffle. The support part 17 is connected to the leakage plate 16. The blocking and pushing part 18 is arranged above the platform base 6 and is used for pushing down the sample bottle 5 that has completed the detection and then entering the leakage holes 19. When the virus detection of the sample bottle 5 on one platform base 6 is completed, under the power output of the stepping motor 7, through the transmission of the gear 11, the annular rack 8 is driven to rotate self, driving the platform base 6 together with the sample bottle 5 to move out of the detection point. The bottle body 28 of the sample bottle 5 stops moving after touching the blocking and pushing part 18 after being moved out, while the platform base 6 at the lower part of the sample bottle 5 still rotates together with the support arm 10, so that the sample bottle 5 is pushed down by the blocking and pushing part 18 and falls into the leakage holes 19, completing the removal work of the sample bottle 5 on the platform base 6. A waste sample box 31 is preferably placed directly below the leakage holes 19. The waste sample box 31 is a box-shaped structure with an open upper part. After the sample bottle 5 is pushed down, it falls into the waste sample box 31 as Figure 1 shown.

[0047] In some embodiments, in order to make the removal work of the above sample bottle 5 on the platform base 6 smoother, it is preferred to use the platform base 6 as Figures 6 - 8 shown, and the leakage holes 19 are arranged at intervals on the opening side of the C-shaped notch 20. The blocking and pushing part 18 is provided with a V-shaped opening 21. The orientation of the V-shaped opening 21 is the same as the orientation of the C-shaped notch 20, as Figure 4As shown. After the sample bottle 5 is pushed down by the blocking and pushing part 18, it topples in the opening direction towards the C-shaped notch 20 and falls into the leakage hole 19; the V-shaped opening 21 of the blocking and pushing part 18 is arranged. On the one hand, it increases the contact area with the sample bottle 5 and reduces the instantaneous pressure when the sample bottle 5 touches the blocking and pushing part 18; on the other hand, after the sample bottle 5 is pushed down, the two side edges of the V-shaped opening 21 can limit the toppling direction of the sample bottle 5, so that the sample bottle 5 smoothly topples and falls into the leakage hole 19, and then falls through the leakage hole 19 to the waste sample box 31 located below it as shown in Figure 1 shown.

[0048] In some embodiments, as Figure 4 shown, the supporting part 17 and the blocking and pushing part 18 are preferably plate-like structures respectively. The supporting part 17 and the blocking and pushing part 18 are connected to form an inverted L-shaped structure. Among them, the supporting part 17 is arranged at the outermost end far from the supporting arm 10, and the blocking and pushing part 18 is arranged at intervals above the platform base 6, so that the annular rack 8 drives the supporting arm 10 and the platform base 6 to rotate without obstruction.

[0049] In some embodiments, as Figure 5 shown, the bottom periphery of the bottle body 28 is preferably provided with a rounded corner structure 30. When the sample bottle 5 is pushed down, on the one hand, it makes the sample bottle 5 easier to topple; on the other hand, it protects the bottom periphery of the sample bottle 5 from being damaged.

[0050] In some embodiments, as Figure 4 、 Figures 10 - 12 shown, the detection mechanism is also preferably provided with a detection cover 32. The detection cover 32 serves as the above-mentioned detection point and is arranged between the grating 3 and the spectrometer 4. The bottom surface of the detection cover 32 is provided with a through groove opening downward, so that the detection cover 32 is integrally in an n-shaped structure. The through groove serves as the passage for the sample bottle 5 and its platform base 6 to enter and exit. The through groove includes a connected sample bottle passage 33 and a platform base passage 34. One light beam through hole 35 is respectively opened on the left and right side walls of the sample bottle passage 33, and the two light beam through holes 35 are arranged opposite to each other; the platform base passage 34 is arranged at the lower part of the sample bottle passage 33, and the width of the platform base passage 34 is greater than the width of the sample bottle passage 33. After the platform base 6 of the sample bottle 5 enters the platform base passage 34, a detection space is formed with the detection cover 32, and the sample bottle 5 is arranged in this detection space. As Figure 11As shown in the figure, the sample vial channel 33 and the platform base channel 34 are arc-shaped and are consistent with the running trajectories of the sample vial 5 and the platform base 6. Under the power output of the stepper motor 7, through the transmission of the gear 11, the annular rack 8 is driven to rotate, thereby driving the support arm 10 together with the platform base 6 on the support arm 10 to rotate around the central axis of the annular rack 8, driving the sample vial 5 on one platform base 6 into the through slot. The sample vial 5 stops at the position where the two light beam through holes 35 are arranged opposite to each other. At this time, the stable light beam with a fixed wavelength generated by the light source 1 passes through the slit 2 to obtain a light beam with an appropriate intensity, and then passes through the grating 3 to obtain a high-purity monochromatic light beam. The monochromatic light beam enters one light beam through hole 35, then passes through the sample vial 5 and the liquid reagent to be measured inside it to generate a transmitted light beam, enters the spectrometer 4 through the other light beam through hole 35 and can be converted into an intensity signal, and the spectrometer 4 detects the intensity value of the transmitted light beam; after the virus detection is completed, driven by the stepper motor 7, the platform base 6 together with the sample vial 5 is moved out of the detection cover 32. At the same time, the next platform base 6 together with the sample vial 5 to be tested for virus is moved into the detection cover 32 for virus detection, realizing automatic sample injection and sampling, which is more convenient and fast. As shown by Figure 4 As shown, during the process of the annular rack 8 driving the support arm 10 together with the platform base 6 on the support arm 10 to rotate around the central axis of the annular rack 8, the support arm 10 together with the platform base 6 on the support arm 10, the light source 1, the slit 2, the grating 3, and the detection cover 32 are all arranged at intervals above the support arm 10, so that the annular rack 8 can drive the support arm 10 to rotate without obstruction and proceed smoothly.

[0051] In some embodiments, by Figure 1 、 Figure 2 As shown, the detection mechanism preferably further includes a housing 36. The housing 36 is in a box structure. The light source 1, the slit 2, the grating 3, the detection mechanism, and the spectrometer 4 are arranged inside the housing 36 to further ensure that the virus detection is not affected by the external environment; a bottle placing window 37 and a bottle taking window 38 are opened on the side wall of the housing 36. The bottle placing window 37 is directly above the bottle taking window 38, and the leakage plate 16 separates the bottle placing window 37 and the bottle taking window 38; the bottle placing window 37 is used to place the sample vial 5 to be tested for virus in the platform base 6, and a waste sample box 31 is placed in the bottle taking window 38. The waste sample box 31 is arranged directly below the leakage hole 19. A touch display screen 39 is also connected to the surface of the housing 36, which is mainly used to display the detection results of the virus in the liquid reagent to be measured.

[0052] In some embodiments, by Figure 3 、 Figure 13As shown in the figure, in order to further improve the automation level of the present invention, the rapid on-site virus detection device is preferably further provided with an intelligent control system, an infrared sensor 22, and a temperature sensor 23. The infrared sensor 22 is arranged on one side of the platform base 6 for detecting whether a sample bottle 5 is placed on the platform base 6; the temperature sensor 23 is connected to the heat sink 14; the intelligent control system is provided with an intelligent control device 24, and the intelligent control device 24 can generally adopt a single-chip microcomputer; the intelligent control device 24 is connected to the infrared sensor 22, the temperature sensor 23, the stepping motor 7, and the semiconductor refrigeration sheet 15 through control lines respectively.

[0053] When it is detected that the sample bottle 5 is placed on the platform base 6, the infrared sensor 22 generates a high-level electrical signal, which is transmitted to the intelligent control device 24 through the control line. After receiving the high-level electrical signal, the internal timer of the intelligent control device 24 starts timing, and sends an instruction to the stepping motor 7 through the control line. After receiving the instruction, the stepping motor 7 drives the ring rack 8 to rotate self. The self-rotation speed is determined according to the distance of the sample bottle 5 from the detection point and the set time. After the set time (i.e., the time required for the reagent chemical reaction), the internal timer sends a feedback signal to the intelligent control device 24. The intelligent control device 24 sends an instruction to the stepping motor 7 through the control line. After receiving the instruction, the stepping motor 7 stops driving. The sample bottle 5 reaches the detection point for detection. The spectrometer 4 detects the intensity value of the transmitted light beam and transmits the signal to the intelligent control device 24 through the control line. After obtaining the signal generated by the spectrometer 4, the intelligent control device 24 calculates the number of viruses in the liquid reagent to be measured in the sample bottle 5 according to the internal virus quantity - light intensity value standard curve, and completes the detection of the viruses in the liquid reagent to be measured in the sample bottle 5. When there are multiple sample bottles 5, each sample bottle 5 reaches the detection point after the set time of the internal timer in turn, and the detection of the viruses in the liquid reagent to be measured in the sample bottle 5 is completed. The working process is as above and will not be elaborated here.

[0054] The temperature sensor 23 monitors the temperature of the heat sink 14 and transmits the temperature induction signal to the intelligent control device 24 through the control line. After receiving the induction signal, when the temperature of the heat sink 14 is higher or lower than the preset temperature, the intelligent control device 24 sends a refrigeration instruction to the semiconductor refrigeration sheet 15 through the control line. After receiving the instruction, the semiconductor refrigeration sheet 15 controls the cooling and heating intensity to achieve the purpose of controlling the temperature.

[0055] The present invention further includes an input module 40, a display module 41, and a power supply module 42. The intelligent control device 24 is connected to the input module 40, the display module 41, and the power supply module 42 respectively through control lines. The input module 40 includes a keyboard and a touch display screen 39. By inputting a control instruction on the keyboard or the touch display screen 39, the control instruction is sent into the intelligent control device 24, and the input of information of the intelligent control device 24 realizes its corresponding output control respectively. The display module 41 includes the touch display screen 39, which is mainly used for displaying the detection results of the liquid reagent virus to be detected. The power supply module 42 is mainly used for supplying power to components such as the intelligent control system, the infrared sensor 22, the temperature sensor 23, the stepping motor 7, the semiconductor refrigeration chip 15, the keyboard, and the touch display screen 39.

[0056] As a further preferred embodiment, as Figure 2 , Figure 3 shown, a sample placement area 43 is preferably provided at the bottle placement window 37 near the side wall of the outer shell 36. The sample placement area 43 can accommodate three platform bases 6. An infrared sensor 22 is respectively provided corresponding to each platform base 6. The infrared sensor 22 is installed at the bottom of the inner cavity of the outer shell 36 and is used for respectively detecting whether a sample bottle 5 is placed on the platform base 6 rotated into the sample placement area 43, and transmitting the induction signal to the intelligent control device 24 through the control line. According to the actual situation, a sample bottle 5 can be placed on any one of the three platform bases 6. The purpose of setting three platform bases 6 is to enhance the error tolerance of the placement of the sample bottle 5, improve the placement efficiency of the sample bottle 5, and ensure that the sample bottle 5 can be easily placed into the platform base 6 during the transmission of the annular rack 8. A plurality of support arms 10 are arranged at intervals on the annular rack 8 and are distributed in a circular array centered on the central axis of the annular rack 8. A platform base 6 is connected to each support arm 10, and the platform bases 6 are also distributed in a circular array centered on the central axis of the annular rack 8.

[0057] An infrared sensor 22 is installed on the side of each of the above three platform bases 6. When the sample bottle 5 is placed inside the platform base 6, the infrared sensor 22 will generate a high-level electrical signal. The infrared sensor 22 generates a high-level electrical signal and transmits it to the intelligent control device 24 through the control circuit. After receiving the high-level electrical signal, the internal timer of the intelligent control device 24 starts timing and sends an instruction to the stepper motor 7 through the control circuit. After receiving the instruction, the stepper motor 7 drives the ring gear 8 to rotate by a set angle. If the platform base 6 is set to N (N>0 and is a positive integer), then the set angle value is 360 / N. After completing the rotation of this set angle, the intelligent control device 24 sends an instruction to stop rotating to the stepper motor 7 through the control circuit. After receiving the instruction, the stepper motor 7 stops rotating, and the sample bottle 5 at the detection point is subjected to virus detection. The set time for the stepper motor 7 to stop rotating is the same as the virus detection time of the sample bottle 5. After this set time arrives, the intelligent control device 24 sends an instruction to the stepper motor 7 through the control circuit. After receiving the instruction, the stepper motor 7 drives the ring gear 8 to rotate by the set angle value of 360 / N in the same direction as before. At this time, the sample bottle 5 that has completed the detection rotates out of the detection point, and the adjacent sample bottle 5 to be detected rotates into the detection point. According to the above process, the intelligent control device 24 sends an instruction to the stepper motor 7 through the control circuit at intervals of the above set time. After receiving the instruction, the stepper motor 7 drives the ring gear 8 to rotate by the set angle value of 360 / N in the same direction as before. During the period when the ring gear 8 stops rotating, the operator can take out the sample bottle 5 that has completed the virus detection from the platform base 6 and place the sample bottle 5 to be detected into the platform base 6 in the sample placement area 43. The time is preset in the internal timer of the intelligent control device 24. After reaching the set time, a feedback signal is sent from the internal timer to the intelligent control device 24. After receiving the signal, the intelligent control device 24 sends a corresponding instruction to the stepper motor 7.

[0058] It should be noted that the entire light beam propagation process can be carried out in a dark room. Especially, the sample bottle 5 should be in a dark room state at the detection point so that the transmitted light beam formed by passing through the sample bottle 5 and the liquid reagent to be measured inside it is not affected by the external environment. Between the light source 1 and the slit 2, between the slit 2 and the grating 3, between the grating 3 and one side of the bottle body 28 of the sample bottle 5, and between the other side of the bottle body 28 of the sample bottle 5 and the spectrometer 4 can also be respectively connected by optical fibers; the light beam incident from one side of the bottle body 28 of the sample bottle 5 passes through the sample bottle 5 and the liquid reagent to be measured inside it to form a transmitted light beam and enters the optical fiber on the other side of the bottle body 28 of the sample bottle 5; the above light beam generated by the light source 1 is transmitted from the air to be transmitted in the optical fiber, making the light beam transmission more stable and reliable, and further improving the accuracy of the detection data of the spectrometer 4. Preferably, the joints of the optical fibers on both sides of the bottle body 28 of the sample bottle 5 with the bottle body 28 of the sample bottle 5 are symmetrically arranged opposite to each other, and the connection line between the two intersects the central axis of the bottle body 28 of the sample bottle 5.

[0059] The present invention also provides the application of the above-mentioned rapid on-site virus detection device in detecting coronavirus. The present invention does not rely on existing professional detection sites, and can be installed in public places such as airports and stations for rapid detection of coronavirus. Detection can be carried out on-site, greatly shortening the detection process, so as to meet the diagnostic needs of a large number of suspected infected persons and quarantined observers, which is of great significance.

[0060] The above are only the preferred embodiments of the present application, and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A virus on-site rapid detection device, the virus on-site rapid detection device is provided with a light source (1), characterized in that: The on-site rapid virus detection device is also provided with a slit (2), a grating (3), a detection mechanism, and a spectrometer (4); the detection mechanism is provided with a sample bottle (5) for containing a liquid reagent to be detected; the light source (1) outputs a stable light beam with a fixed wavelength, the stable light beam passes through the slit (2) and enters the grating (3), and after being split by the grating (3), passes through the sample bottle (5) and the liquid reagent to be detected therein to form a transmitted light beam, and the transmitted light beam enters the spectrometer (4) for detection of light intensity.

2. A virus on-site rapid detection device according to claim 1, characterized in that: The width of the slit (2) is adjustable, and the adjustment range is 0 to 5 mm.

3. A virus on-site rapid detection device according to claim 1, characterized in that: The detection mechanism is also provided with a platform base (6), and the platform base (6) is used to place the sample bottle (5).

4. A virus on-site rapid detection device according to claim 3, characterized in that: The detection mechanism is also provided with a stepper motor (7) and a transmission device, wherein the transmission device is provided with an annular rack (8), an annular bracket (9), a support arm (10), and a gear (11); the annular rack (8) is coaxially sleeved in the annular bracket (9), the teeth of the annular rack (8) are arranged on the inner side of the annular shape of the annular rack (8), one end of the support arm (10) is connected to the annular rack (8), the other end of the support arm (10) is extended toward the annular bracket (9), and the arm body of the support arm (10) is slidably connected to the annular bracket (9); the power output shaft of the stepper motor (7) is connected to the gear (11), and the gear (11) is meshed with the annular rack (8); the number of the support arms (10) is set to be multiple, and the multiple support arms (10) are arranged on the annular rack (8) at intervals, and each support arm (10) is connected to a platform base (6).

5. A virus on-site rapid detection device according to claim 4, characterized in that: The arm body of the support arm (10) is connected to a slider (12), and the annular bracket (9) is provided with a circumferential slideway (13) matching the slider (12).

6. A virus on-site rapid detection device according to claim 4, characterized in that: A thermostatic component is also provided on the outside of the annular support (9), and the thermostatic component is provided with a heat sink (14) and a semiconductor cooling fin (15) which are connected to each other. The other end of the support arm (10) faces the annular support (9) and extends above the thermostatic component. The platform base (6) is arranged above the heat sink (14).

7. A virus on-site rapid detection device according to claim 6, characterized in that: The detection mechanism is also provided with an ejection device, the ejection device is provided with a baffle and a leak plate (16), the baffle is provided with a supporting portion (17) and a blocking and pushing portion (18) which are connected to each other, the leak plate (16) is provided with a leak hole (19), and the leak hole (19) is arranged on one side of the baffle; the supporting portion (17) is connected to the leak plate (16), and the blocking and pushing portion (18) is arranged above the platform base (6) and is used to push down the sample bottle (5) that has completed the test and enter the leak hole (19).

8. A virus on-site rapid detection device according to claim 7, characterized in that: The platform base (6) is provided with a C-shaped notch (20) for placing the sample bottle (5), and the leak holes (19) are arranged at intervals on one side of the opening of the C-shaped notch (20); the push-blocking portion (18) is provided with a V-shaped opening (21), and the direction of the V-shaped opening (21) is consistent with the direction of the C-shaped notch (20).

9. A virus on-site rapid detection device according to claim 6, characterized in that: The on-site rapid virus detection device is also provided with an intelligent control system, an infrared sensor (22), and a temperature sensor (23). The infrared sensor (22) is arranged on one side of the platform base (6) and is used to detect whether the sample bottle (5) is placed on the platform base (6); the temperature sensor (23) is connected to the heat sink (14); the intelligent control system is provided with an intelligent control device (24), and the intelligent control device (24) is respectively connected to the infrared sensor (22), the temperature sensor (23), the stepping motor (7), and the semiconductor cooling plate (15) through a control circuit.

10. Use of the on-site rapid virus detection device according to any one of claims 1 to 9 in detecting coronavirus.

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