Traditional Chinese medicine composition for treating chronic headache and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine composition for treating chronic headache and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 1200-1300 parts of ligusticum wallichii, 570-670 parts of radix paeoniae alba, 450-550 parts of radix puerariae, 60-500 parts of vinegar-processed rhizoma corydalis, 330-420 parts of honey bran stiff silkworm, 320-420 parts of vinegar-processed rhizoma cyperi and 200-300 parts of radix angelicae. The traditional Chinese medicine composition is reasonable in formula and simple in medicine flavor, avoids waste of traditional Chinese medicine materials, is remarkable in curative effect, has a remarkable regulation effect on headache related indexes, and can relieve central sensitization of rats with chronic migraine. And compared with flunarizine hydrochloride and traditional Chinese medicines for relieving headache, the traditional Chinese medicine composition also has better effects and advantages.

Description

Technical Field

[0001] The present invention belongs to the technical field of natural medicine chemistry, and particularly relates to a traditional Chinese medicine composition for treating chronic headache and a preparation method thereof. Background Art

[0002] Chronic headache is a common neurological disease. Among them, migraine is the most common type of chronic headache, followed by tension headache. In clinical treatment, it is found that chronic headache often coexists with anxiety, depression, sleep disorders, etc. Some studies also find that it may increase the risk of cognitive impairment and cardiovascular and cerebrovascular diseases. In addition, the treatment and care costs of chronic headache are high, which not only seriously affects the quality of life of patients, but also has a significant impact on social economy.

[0003] With the development of Western medicine, there are various theories about the pathogenesis of chronic headache. It mainly includes the vascular theory, neuron theory (cortical spreading depression theory, trigeminovascular theory), inflammatory mediator theory, etc. Currently, the commonly used drugs in clinical practice, such as non-steroidal anti-inflammatory drugs, calcium channel antagonists, anti-epileptic drugs, β-blockers, calcium channel modulators, antidepressants, etc., all have certain therapeutic and preventive effects on chronic headache. Among them, non-steroidal anti-inflammatory drugs are the most widely used drugs for migraine treatment, mainly including ibuprofen, diclofenac, aspirin, and naproxen. Its effectiveness has been proven. Especially for mild to moderate pain attacks, 76% of patients can be completely relieved by using drugs in the acute phase. Flunarizine is a preventive drug with a relatively strong evidence level. Although there are an endless stream of drugs for treating chronic headache with Western medicine, there is no Western medicine with a specific effect. And due to the long course of chronic headache, most Western medicines will have adverse reactions such as gastrointestinal reactions, extrapyramidal reactions, and cognitive impairment. Long-term use will also have the risk of liver and kidney damage, increasing the pain of patients. Therefore, finding a treatment drug with long-term, stable, small side effects and good curative effect has gradually become the research direction of current chronic headache treatment.

[0004] Traditional Chinese medicine treats chronic headache, formulating prescriptions according to syndromes and prescribing formulas according to the methods, with good clinical curative effect, small side effects, and most patients having good compliance and being easy to accept traditional Chinese medicine treatment. Therefore, traditional Chinese medicine has obvious advantages in treating chronic headache, so it is urgent to develop new traditional Chinese medicine drugs for treating chronic headache. Summary of the Invention

[0005] Aiming at the problems existing in the prior art, the purpose of the design of the present invention is to provide a traditional Chinese medicine composition for treating chronic headache and a preparation method thereof, which are specifically realized through the following technical solutions:

[0006] A traditional Chinese medicine composition for treating chronic headache, which is composed of the following components in parts by weight:

[0007] 1200 - 1300 parts of Ligusticum chuanxiong, 570 - 670 parts of Paeonia lactiflora, 450 - 550 parts of Pueraria lobata, 60 - 500 parts of Corydalis yanhusuo processed with vinegar, 330 - 420 parts of Bombyx batryticatus processed with honey bran, 320 - 420 parts of Cyperus rotundus processed with vinegar, 200 - 300 parts of Angelica dahurica.

[0008] A preparation method of a traditional Chinese medicine composition for treating chronic headache, comprising the following steps:

[0009] 1) Take the formula amounts of Ligusticum chuanxiong and Cyperus rotundus processed with vinegar, use 5 times the amount of water as a solvent for impregnation, extract by steam distillation, collect the volatile oil, and wrap the volatile oil with a saturated β - cyclodextrin solution for later use;

[0010] 2) Centrifuge and separate the impurities from the extraction liquid in step 1) for later use;

[0011] 3) Take the medicinal residues after volatile oil extraction in step 1), add the formula amounts of Paeonia lactiflora, Pueraria lobata, and Bombyx batryticatus processed with honey bran, use 10 times the amount of water as a solvent, reflux extract three times, each time for 1 hour, combine the extraction liquids, centrifuge and separate the impurities from the extraction liquid and then concentrate it into the first clear paste;

[0012] 4) Crush the Corydalis yanhusuo processed with vinegar slices and sieve them through a No. 3 sieve. Take the formula amounts of Corydalis yanhusuo powder and Angelica dahurica, use 7 times the amount of 60% ethanol as a solvent, reflux extract twice, each time for 1.5 hours, combine the extraction liquids, remove the impurities from the extraction liquid with a plate - frame filter, and then concentrate it into the second clear paste;

[0013] 5) Combine the first clear paste and the second clear paste, spray - dry to obtain a dry powder, add the volatile oil wrapped with the β - cyclodextrin solution prepared in step 1), add auxiliary materials, and prepare granules by dry granulation.

[0014] Further, in step 1), impregnate for 0.5 h and perform steam distillation for 9.5 h.

[0015] Further, in step 1), the specific steps for wrapping the volatile oil with a saturated β - cyclodextrin solution are as follows: Prepare an ethanol solution of the volatile oil with a volume ratio of volatile oil:ethanol of 1:4; Take β - CD, add water according to a material - liquid ratio of 1 g:10 mL, stir at 60 °C for 10 min, cool to room temperature to prepare a β - CD saturated solution, then transfer it to a constant - temperature magnetic stirrer, and add the ethanol solution of the volatile oil drop - by - drop under stirring according to a volume ratio of β - cyclodextrin:volatile oil = 8:1. The magnetic stirring speed is 300 r / min, the temperature is 40 °C, perform inclusion for 180 min until it is in a suspension state, cool to room temperature, place it in the refrigerator for refrigeration for 24 h, then filter, wash it once with pure water, and let the obtained inclusion compound dry naturally to obtain a dry inclusion compound.

[0016] Further, in step 3), the relative density of the first clear paste is 1.10 ± 0.02 g / cm 3 , and the concentration temperature is 40 - 50 °C.

[0017] Further, in step 4), the reflux extraction temperature is 85°C, and the relative Density is 1.00 ± 0.02 g / cm 3 , the concentration temperature is 40 - 50 °C.

[0018] Further, in step 5), the conditions for spray drying are an inlet air temperature of 178°C, a peristaltic speed of 12 rpm, and a relative density of the liquid medicine of 1.07 g / cm 3 .

[0019] Further, in step 5), the excipients are selected as dextrin and magnesium stearate. The addition amount of dextrin accounts for 5% of the total amount, and the addition amount of magnesium stearate accounts for 0.5% of the total amount.

[0020] The traditional Chinese medicine composition of the present invention has a reasonable formula, concise medicinal properties, avoids the waste of traditional Chinese medicine materials, and has a significant curative effect, has a significant regulatory effect on headache-related indicators, and can relieve the central sensitization of chronic migraine rats. It also has better effects and advantages compared with flunarizine hydrochloride and traditional Chinese medicine drugs for relieving headache. Description of the Drawings

[0021] Figure 1 is the HPLC chromatogram of ferulic acid reference substance solution;

[0022] Figure 2 is the HPLC chromatogram of ferulic acid in the test solution;

[0023] Figure 3 is the HPLC chromatogram of paeoniflorin reference substance solution;

[0024] Figure 4 is the HPLC chromatogram of paeoniflorin in the test solution;

[0025] Figure 5 is the HPLC chromatogram of puerarin reference substance solution;

[0026] Figure 6 is the HPLC chromatogram of puerarin in the test solution;

[0027] Figure 7 is the HPLC chromatogram of tetrahydropalmatine reference substance solution;

[0028] Figure 8 is the HPLC chromatogram of tetrahydropalmatine in the test solution;

[0029] Figure 9 is the HPLC chromatogram of imperatorin reference substance solution;

[0030] Figure 10 is the HPLC chromatogram of imperatorin in the test solution. Detailed Embodiments

[0031] The present invention will be further described below in conjunction with specific embodiments for better understanding of the technical solution.

[0032] Example 1

[0033] A preparation method of a traditional Chinese medicine composition for treating chronic headache:

[0034] Take 1240.7 g of Ligusticum chuanxiong and 372.2 g of Cyperus rotundus vinegar-processed, use 5 times the amount of water as the solvent, soak for 0.5 h, steam distill for 9.5 h, extract and collect the volatile oil, wrap the volatile oil with a saturated β-cyclodextrin solution, and set aside separately. And obtain the extracted medicinal liquid, and set aside the extract after centrifugal separation to remove impurities.

[0035] Take the medicinal residues after extracting the volatile oil of Ligusticum chuanxiong and Cyperus rotundus vinegar-processed, add 620.3 g of Paeonia lactiflora, 496.3 g of Pueraria lobata, and 372.2 g of Bombyx batryticatus stir-fried with bran and honey, use 10 times the amount of water as the solvent, reflux extract 3 times, 1 h each time. After the extract is centrifugally separated to remove impurities, it is concentrated into a clear paste with a relative density of 1.10±0.02 (40 - 50 °C). Separately take 496.3 g of Corydalis yanhusuo vinegar-processed slices, crush them and pass through a No. 3 sieve. Take the powder of Corydalis yanhusuo vinegar-processed and 620.3 g of Angelica dahurica, use 7 times the amount of 60% ethanol as the solvent, reflux extract at 85 °C for 2 times, 1.5 h each time. After removing impurities from the extract with a plate and frame filter, it is concentrated into a clear paste with a relative density of 1.00±0.02 (40 - 50 °C).

[0036] Merge the above clear pastes, spray dry to obtain a dry powder, add the volatile oil wrapped with a β-cyclodextrin solution, and then add an appropriate amount of dextrin and magnesium stearate, and make granules by the dry method to obtain the product.

[0037] Verification Example 1:

[0038] 1.1 Quality inspection

[0039] According to the requirements of the fourth part of the Chinese Pharmacopoeia 2020 edition, the products prepared in Example 1 were inspected for particle size, moisture, solubility, and filling quantity difference. The inspection results all met the regulations, and the results are shown in Tables 1 - 4.

[0040] Table 1: Particle size inspection results

[0041]

[0042] Table 2: Moisture inspection results

[0043]

[0044]

[0045] Table 3: Solubility inspection results

[0046]

[0047] Table 4: Inspection of variation in filling quantity

[0048]

[0049] 1.2 Inspection of heavy metals and arsenic salts

[0050] The inspection of heavy metals and arsenic salts was carried out according to the method of inductively coupled plasma atomic emission spectrometry. The results showed that the heavy metal content of the three batches of granules was less than 7 ppm, and the arsenic salt content was less than 1.8 ppm. Therefore, they were not included in the quality standard.

[0051] In the preliminary pre-test, the inspection results of the three batches of samples all met the requirements.

[0052] 1.3 Microbiological limit

[0053] Inspection was carried out according to the method for the inspection of microbiological limit of non-sterile products in Part IV of the Chinese Pharmacopoeia (2020 Edition). After methodological verification, the pre-test of this product was carried out by the culture medium dilution method, and the results showed that the microbiological limit met the requirements.

[0054] 1.4 Content: For the determination of content, an HPLC method was established with ferulic acid, paeoniflorin, puerarin, tetrahydropalmatine and imperatorin as indicators. After methodological investigation and verification, the established method is as follows:

[0055] 1.4.1 Ferulic acid

[0056] Determination was carried out with reference to the high performance liquid chromatography method (Part I of the Chinese Pharmacopoeia 2020 Edition).

[0057] Chromatographic conditions and system suitability test: Octadecylsilane chemically bonded silica gel was used as the filler; methanol - 1% acetic acid solution (30:70) was used as the mobile phase; the detection wavelength was 321 nm. The number of theoretical plates calculated with reference to the ferulic acid peak should be not less than 4000.

[0058] Preparation of reference substance solution: Appropriate amount of ferulic acid reference substance was accurately weighed and placed in a brown volumetric flask, and 70% methanol was added to make a solution containing 20 μg per 1 ml, that is obtained.

[0059] Preparation of test solution: The content of this product was ground finely, about 0.5 g was accurately weighed and placed in a stoppered conical flask, 50 ml of 70% methanol was accurately added, the stopper was closed, the weight was weighed, heated under reflux for 30 minutes, cooled, weighed again, and the lost weight was made up with 70% methanol, shaken well, allowed to stand, the supernatant was taken, filtered, and the subsequent filtrate was taken, that is obtained.

[0060] Determination method: 10 μl of the reference substance solution and the test solution were accurately pipetted respectively, injected into the liquid chromatograph for determination, and that is obtained. The liquid chromatogram is shown in Figure 1-2 .

[0061] 1.4.2 Paeoniflorin

[0062] Determined with reference to the high performance liquid chromatography (Volume I of Chinese Pharmacopoeia 2020 Edition).

[0063] Chromatographic conditions and system suitability test: Using octadecylsilane chemically bonded silica gel as the filler; using acetonitrile - 0.1% phosphoric acid solution (14:86) as the mobile phase; the detection wavelength is 230 nm. The number of theoretical plates calculated with reference to the paeoniflorin peak should be not less than 2000.

[0064] Preparation of reference substance solution: Weigh an appropriate amount of paeoniflorin reference substance accurately, dissolve it in methanol to prepare a solution containing 60 μg per 1 ml, and that is what is required.

[0065] Preparation of test solution: Take the contents of this product, grind it finely, weigh about 0.5 g accurately, place it in a 50 ml volumetric flask, add 35 ml of dilute ethanol, ultrasonically treat it (power 240 W, frequency 45 kHz) for 30 minutes, let it cool, add dilute ethanol to the scale, shake well, filter, and take the subsequent filtrate, and that is what is required.

[0066] Determination method: Precisely pipette 10 μl each of the reference substance solution and the test solution, inject them into the liquid chromatograph for determination, and that is what is obtained. The liquid chromatogram is shown in Figure 3-4 .

[0067] 1.4.3 Puerarin

[0068] Determined with reference to the high performance liquid chromatography (Volume I of Chinese Pharmacopoeia 2020 Edition).

[0069] Chromatographic conditions and system suitability test: Using octadecylsilane chemically bonded silica gel as the filler; using methanol - water (25:75) as the mobile phase; the detection wavelength is 250 nm. The number of theoretical plates calculated with reference to the puerarin peak should be not less than 4000.

[0070] Preparation of reference substance solution: Weigh an appropriate amount of puerarin reference substance accurately, dissolve it in 30% ethanol to prepare a solution containing 80 μg per 1 ml, and that is what is required.

[0071] Preparation of test solution: Take about 0.1 g of the powder of this product (passing through No. 3 sieve), weigh it accurately, place it in a stoppered conical flask, accurately add 50 ml of 30% ethanol, weigh it, heat under reflux for 30 minutes, let it cool, weigh it again, make up the lost weight with 30% ethanol, shake well, filter, and take the subsequent filtrate, and that is what is required.

[0072] Determination method: Precisely pipette 10 μl each of the reference substance solution and the test solution, inject them into the liquid chromatograph for determination, and that is what is obtained. The liquid chromatogram is shown in Figure 5-6 .

[0073] 1.4.4 Tetrahydropalmatine

[0074] Chromatographic conditions and system suitability test: Use octadecylsilane chemically bonded silica gel as the filler; use methanol - 0.1% phosphoric acid solution (adjust the pH value to 6.0 with triethylamine) (55∶45) as the mobile phase; the detection wavelength is 280 nm. The number of theoretical plates calculated based on the peak of tetrahydropalmatine should be not less than 3000.

[0075] Preparation of reference solution: Weigh an appropriate amount of tetrahydropalmatine reference substance accurately, dissolve it in methanol to prepare a solution containing 46 μg per 1 ml, and that is the reference solution.

[0076] Preparation of test solution: Take about 0.5 g of the powder of this product (passed through No. 3 sieve), weigh it accurately, place it in a flat-bottomed flask, accurately add 50 ml of the mixed solution of concentrated ammonia test solution - methanol (1∶20), weigh it, soak it in the cold for 1 hour and then heat it under reflux for 1 hour, cool it, weigh it again, make up the lost weight with the mixed solution of concentrated ammonia test solution - methanol (1∶20), shake well, filter. Accurately measure 25 ml of the continued filtrate, evaporate to dryness, dissolve the residue in methanol, transfer it to a 5-ml volumetric flask, dilute it to the scale, shake well, filter, and take the continued filtrate, and that is the test solution.

[0077] Assay method: Accurately pipette 10 μl each of the reference solution and the test solution, inject them into the liquid chromatograph for determination, and that is the result. The liquid chromatogram is shown in Figure 7-8 。

[0078] 1.4.5 Imperatorin

[0079] Chromatographic conditions and system suitability test: Use octadecylsilane chemically bonded silica gel as the filler; use methanol - water (55:45) as the mobile phase; the detection wavelength is 300 nm. The number of theoretical plates calculated based on the peak of imperatorin should be not less than 3000.

[0080] Preparation of reference solution: Weigh an appropriate amount of imperatorin reference substance accurately, dissolve it in methanol to prepare a solution containing 10 μg per 1 ml, and that is the reference solution.

[0081] Preparation of test solution: Take about 0.4 g of the powder of this product (passed through No. 3 sieve), weigh it accurately, place it in a 50-ml volumetric flask, add 45 ml of methanol, ultrasonically treat it (power 300 W, frequency 50 kHz) for 1 hour, take it out, cool it, add methanol to the scale, shake well, filter, and take the continued filtrate, and that is the test solution.

[0082] Assay method: Accurately pipette 10 μl each of the reference solution and the test solution, inject them into the liquid chromatograph for determination, and that is the result. The liquid chromatogram is shown in Figure 9-10 。

[0083] Verification Example 2: Animal experiment

[0084] Nitroglycerin-induced migraine rat model: The nitroglycerin-induced migraine model is economical and easy to replicate, and does not require craniotomy, facilitating the observation of the behaviors of migraine model rats with clear consciousness. It is a classic model for studying the effects of drugs on migraine at present.

[0085] 2.1 Model preparation

[0086] The method for constructing a nitroglycerin-induced migraine rat model was adopted. That is, on the mornings of the 1st, 3rd, 5th, 7th, and 9th days at the beginning of the experiment, nitroglycerin injection (10 mg / kg) was intraperitoneally injected, with a total of 5 administrations; the nitroglycerin injection was prepared and used immediately. The stock solution (concentration 5 mg / ml, components including 30% ethanol, 30% propylene glycol, and water) was diluted with 0.9% normal saline to a concentration of 1 mg / ml and then injected. It was stored in the dark at low temperature. Rats intraperitoneally injected with the same dose of normal saline were used as the blank group. The successful preparation of the migraine model was indicated by symptoms such as listlessness, reduced activity, red ears, and frequent scratching of the front paws by the rats.

[0087] 2.2 Grouping and administration

[0088] After 7 days of adaptive feeding of SPF-grade rats, they were randomly divided into a blank group, a model group, and the following administration groups, a total of 185 groups.

[0089] 1. Group 1 of the present invention: 30 g of Ligusticum chuanxiong, 15 g of Paeonia lactiflora, 12 g of Pueraria lobata, 12 g of Corydalis yanhusuo vinegar processed, 9 g of Bombyx batryticatus bran stir-fried with honey, 9 g of Cyperus rotundus vinegar processed, 6 g of Angelica dahurica.

[0090] 2. Group 2 of the present invention: 15 g of Ligusticum chuanxiong, 7.5 g of Paeonia lactiflora, 6 g of Pueraria lobata, 6 g of Corydalis yanhusuo vinegar processed, 4.5 g of Bombyx batryticatus bran stir-fried with honey, 4.5 g of Cyperus rotundus vinegar processed, 3 g of Angelica dahurica.

[0091] 3. Group 3 of the present invention: 60 g of Ligusticum chuanxiong, 30 g of Paeonia lactiflora, 24 g of Pueraria lobata, 24 g of Corydalis yanhusuo vinegar processed, 18 g of Bombyx batryticatus bran stir-fried with honey, 18 g of Cyperus rotundus vinegar processed, 12 g of Angelica dahurica.

[0092] 4. Control group 1: Application number CN201810396228.5 A Chinese medicine formula for treating migraine: Lithospermum officinale 20g, Cassia seed 20g, Gypsum 20g, Mother of Pearl 20g, White Peony 12g, Gastrodia 9g, Pueraria 15g, Gentiana 9g, Chrysanthemum 15g, Salvia miltiorrhiza 15g, Red Peony 10g, Cortex Moutan 15g, Bombyx batryticatus 9g, Cannabis seed 9g, Prunus mume seed 9g, Tortoise shell 20g, Prunella vulgaris 15g, Amomum villosum 9g, Arisaema cinerea 9g, Tangerine peel 9g, Acorus calamus 10g, Whole worm 9g, Dragon 9g, Chuanxiong 15g, peach kernel 8g, safflower 9g, windproof 9g, Angelica dahurica 10g, Qianghuo 9g, Vitex 12g, Ligusticum 9g, Bamboo Shavings 15g, Phragmites australis 15g, Achyranthes bidentata 15g, Uncaria rhynchophylla 12g, processed Chuanwu 10g, Angelica 15g, Asarum 9g, Atractylodes 12g, Poria 15g, Antelope Horn 12g, Yuanhu 15g, Astragalus 20g, Cyperus 9g, Curcuma 9g, Morus 15g, Morus 15g, Artemisia capillaris 9g, Radish Seed 9g, Bupleurum 15g, Luffa loofah 15g and Stretching Grass 15g.

[0093] 5. Control group 2: Authorization number CN103083494B, a traditional Chinese medicine for treating vascular headache: Pueraria root, earthworm 9g, Chuanxiong 15g, white peony 12g, Uncaria 12g, chrysanthemum 15g, Corydalis 15g, Asarum 9g, Achyranthes bidentata 15g, Angelica dahurica 10g, Ligusticum 9g, Scorpio 9g, Bombyx batryticatus 9g, Pinellia 9g, and Licorice 8g.

[0094] 6. Control group 3: Authorization number CN101406668B A Chinese medicine composition for treating headache and dizziness and its preparation method: 10-20 parts of Chuanxiong, 10-20 parts of Angelica dahurica, 10-20 parts of Eucommia ulmoides, 10-20 parts of Corydalis yanhusuo, 10-20 parts of Bombyx batryticatus, 10-20 parts of Curcuma aromatica, 10-20 parts of Salvia miltiorrhiza, 10-20 parts of Polygonum multiflorum, 10-20 parts of Zedoariae rhizome, 10-20 parts of Pueraria root, 10-20 parts of Angelica sinensis, 10-20 parts of Semen Cassiae, 10-20 parts of Gastrodia elata, 10-20 parts of Astragalus, 10-20 parts of Cassia twig, 10-20 parts of Chrysanthemum, and 10-20 parts of White Peony.

[0095] 7. Control group 4: Application number CN201910878552.5 A method for preparing a traditional Chinese medicine for treating headache: Radix Dibuxian 9g, Saposhnikovia divaricata 9g, Radix Ligustici Ligustici Radix 9g, Rhizoma Corydalis 15g, Gastrodia elata 9g, Rhizoma Chuanxiong 15g, Bombyx Batryticatus 9g, Angelica dahurica 10g, Rhizoma Notopterygii 9g, Radix Puerariae 15g, Rhizoma Achyranthis Bidentatae 15g and Radix Bupleuri 15g.

[0096] 8. Control group 5: Application number CN201710101989.9 A traditional Chinese medicine composition for treating headache: 3#18 parts of Chuanxiong, 3#18 parts of Bupleurum, 3#18 parts of Salvia miltiorrhiza, 5#25 parts of Corydalis, 3#18 parts of Angelica dahurica, 5#25 parts of White Peony, 3#18 parts of White Sinapis, 3#18 parts of Cyperus rotundus, 3#18 parts of Prunus mume, and 1#10 parts of Licorice.

[0097] 9. Control Group 6: Application No. CN201711102366.X, a pill medicine for treating headache and its preparation method: 90 g of Cynanchum paniculatum, 80 g of Chrysanthemum morifolium, 80 g of Vitex trifolia, 80 g of Gardenia jasminoides, 80 g of Angelica dahurica, 150 g of Ophiopogon japonicus, 100 g of Heracleum hemsleyanum, 100 g of Hippophae rhamnoides, 100 g of Notopterygium incisum, 50 g of Gentiana scabra, 200 g of Rehmannia glutinosa, 80 g of Saposhnikovia divaricata, 90 g of Scutellaria baicalensis, 20 g of Asarum sieboldii, 90 g of Ocimum basilicum, 80 g of Carthamus tinctorius, 80 g of Bupleurum chinense, 80 g of Gastrodia elata, 60 g of Pinellia ternata, 200 g of Astragalus membranaceus, 120 g of Pueraria lobata, 80 g of Ligusticum wallichii, 80 g of Crataegus pinnatifida, 80 g of Aurantii Fructus Immaturus, 120 g of Haliotis diversicolor, 80 g of Xanthium sibiricum, 80 g of Ligusticum sinense, 80 g of Rheum officinale, 80 g of Corydalis yanhusuo, 800 g of Chaenomeles speciosa, 80 g of Bombyx batryticatus.

[0098] 10. Control Group 7: Application No. CN201610787717.4, a pill medicine that can effectively treat headache and its preparation method: 90 - 160 g of Chrysanthemum indicum, 150 - 650 g of Smilax glabra, 50 - 120 g of Angelica sinensis, 50 - 120 g of Vitex trifolia, 60 - 160 g of Ophiopogon japonicus (with removed heart), 25 - 60 g of Cornu saigae tataricae powder, 30 - 100 g of Heracleum hemsleyanum, 100 - 260 g of Gardenia jasminoides, 30 - 120 g of Angelica dahurica, 30 - 120 g of Notopterygium incisum, 60 - 160 g of Paeonia lactiflora, 50 - 120 g of Saposhnikovia divaricata, 30 - 100 g of Scutellaria baicalensis, 15 - 100 g of Asarum sieboldii, 30 - 70 g of Gentiana scabra, 50 - 160 g of Achyranthes bidentata, 90 - 160 g of Rehmannia glutinosa, 50 - 120 g of Carthamus tinctorius, 30 - 100 g of Bupleurum chinense, 100 - 360 g of Astragalus membranaceus, 60 - 100 g of Paeonia suffruticosa, 80 - 160 g of Pueraria lobata, 80 - 160 g of Gastrodia elata, 30 - 100 g of Pinellia ternata, 30 - 120 g of Crataegus pinnatifida, 30 - 120 g of Ligusticum wallichii, 30 - 120 g of Aurantii Fructus Immaturus, 30 - 120 g of Ligusticum sinense, 40 - 120 g of Rheum officinale, 90 - 160 g of Haliotis diversicolor, 50 - 120 g of Leonurus japonicus, 30 - 100 g of Xanthium sibiricum, 600 - 1200 g of Chaenomeles speciosa, 50 - 100 g of Schizonepeta tenuifolia, 30 - 70 g of Sinapis alba, 90 - 160 g of Cassia obtusifolia, 150 - 650 g of Gypsum fibrosum, #50 - 100 g of Bombyx batryticatus, 60 - 160 g of Paeonia lactiflora, 30 - 130 g of Glycyrrhiza uralensis, 90 - 160 g of Trichosanthes kirilowii, 20 - 50 g of Serpent Slough, 15 - 100 g of Panax ginseng, 20 - 50 g of Scorpio, 80 - 160 g of Pueraria lobata, Excipient: refined honey (add 135 - 165 g of refined honey to every 100 g of medicinal powder).

[0099] 11. Control Group 8: A traditional Chinese medicine for treating nervous headache with the application number CN201610787717.4: 10 - 20 parts of Flos Chrysanthemi Indici, 10 - 15 parts of Semen Cassiae, 8 - 12 parts of Fructus Evodiae, 4 - 6 parts of Caulis Bambusae in Taenia, 10 - 20 parts of Rhizoma Chuanxiong, 8 - 12 parts of Rhizoma Gastrodiae, 8 - 12 parts of Herba Asari, 5 - 10 parts of Rhizoma Corydalis, 4 - 6 parts of Fructus Viticis, 5 - 10 parts of Ramulus Cinnamomi, 5 - 15 parts of Folium Ilicis Latifoliae, 5 - 10 parts of Flos Solidaginis Virgaureae, 4 - 6 parts of Radix Schizophragmatis Integrifolii, 1 - 3 parts of Radix Osterici Sibirici, 5 - 7 parts of Semen Trigonellae, 4 - 6 parts of Radix Cynanchi Paniculati, 5 - 15 parts of Radix Angelicae Dahuricae, 8 - 12 parts of Radix Notoginseng, 13 - 17 parts of Radix Puerariae, 8 - 12 parts of Radix Paeoniae Alba, 5 - 10 parts of Salsola collina Pall. and 4 - 6 parts of Cortex Philadelphii Inodori.

[0100] 12. Control Group 9: A traditional Chinese medicine granule for treating menstrual headache with the application number CN201610301948.X: 15 g of Rhizoma Chuanxiong, 15 g of Radix Cyathulae, 12 g of Rhizoma Cyperi, 15 g of Rhizoma Corydalis, 15 g of Herba Leonuri, 15 g of Radix Angelicae Sinensis, 10 g of brown sugar, 15 g of Flos Rosae Rugosae, 15 g of Fructus Hordei Germinatus (parched), 9 g of Herba Dendrobii, 15 g of Arillus Longan, 12 g of Radix Paeoniae Alba, 10 g of Radix Glycyrrhizae Preparata, 15 g of Flos Chrysanthemi, 15 g of Rhizoma Polygonati, 15 g of Radix Bupleuri, 12 g of Radix Scutellariae, 15 g of Fructus Gardeniae (parched), 9 g of Rhizoma Pinelliae Praeparatum, 8 g of Rhizoma Zingiberis Recens, 10 g of Fructus Jujubae, 9 g of Rhizoma Gastrodiae, 15 g of Ramulus Uncariae Cum Uncis, 9 g of Pheretima, 9 g of Rhizoma Ligustici, 9 g of Radix Curcumae, 10 g of Fructus Tribuli Terrestris, 15 g of Radix Puerariae and 15 g of Fructus Evodiae.

[0101] 13. Control Group 10: A decoction drug for treating angioneurotic headache and migraine and its preparation method with the application number CN201610001720.9: 8 g of Radix Bupleuri, 12 g of Radix Notoginseng, 9 g of Rhizoma Chuanxiong, 8 g of Radix Angelicae Dahuricae, 9 g of Herba Angelicae Pubescentis, 12 g of Rhizoma Cyperi, 9 g of Rhizoma Corydalis Yanhusuo (processed with vinegar), 15 g of Radix Paeoniae Alba, 12 g of Semen Pruni, 9 g of Semen Sinapis Albae, 8 g of Radix Glycyrrhizae, 12 g of Cortex Albiziae.

[0102] 14. Control Group 11: A compound traditional Chinese medicine for treating angioneurotic headache with the application number CN201310705777.3: 2 - 8 parts of Radix Salviae Miltiorrhizae, 2 - 8 parts of Radix Ginseng, 8 - 12 parts of Radix Angelicae Dahuricae, 6 - 10 parts of Rhizoma Chuanxiong, 8 - 16 parts of Ramulus Uncariae Cum Uncis, 8 - 12 parts of Rhizoma Gastrodiae, 6 - 10 parts of Herba Ephedrae, 2 - 6 parts of Cortex Cinnamomi, 8 - 16 parts of Pheretima, 2 - 8 parts of Radix Saposhnikoviae, 2 - 6 parts of Rhizoma Cyperi, 10 - 20 parts of Radix Puerariae.

[0103] 15. Control Group 12: A traditional Chinese medicine for treating intractable headache with the application number CN201310203106.7: 25 g of Rhizoma Chuanxiong, 12 g of Radix Angelicae Dahuricae, 9 g of Rhizoma Cyperi Praeparatum, 25 g of Radix Paeoniae Alba, 12 g of Rhizoma Gastrodiae, 12 g of Bombyx Batryticatus, 12 g of Radix Angelicae Sinensis, 8 g of Herba Asari, 9 g of Radix Saposhnikoviae and 9 g of Pheretima.

[0104] 16. Control Group 13: Application No. CN202310240721.9, Use of a Traditional Chinese Medicine Composition in the Preparation of a Medicament for Treating Headache: Bupleurum chinense DC. 15 g, Paeonia lactiflora Pall. 12 g, Cyperus rotundus L. 9 g, Honey-fried Licorice Root 10 g, Ligusticum wallichii Franch. 15 g, Pueraria lobata (Willd.) Ohwi 15 g, Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav. 10 g, Angelica sinensis (Oliv.) Diels 15 g, Chaenomeles speciosa (Sweet) Nakai 15 g.

[0105] 17. Control Group 14: Application No. CN201410485437.9, A Traditional Chinese Medicine Composition for Treating Neural Headache: Chrysanthemum morifolium Ramat. 10 - 20 parts, Corydalis yanhusuo W. T. Wang 10 - 20 parts, Haematitum 15 - 25 parts, Tribulus terrestris L. 15 - 25 parts, Uncaria rhynchophylla (Miq.) Miq. ex Havil. 10 - 15 parts, Concha Margaritifera Usta 25 - 35 parts, Atractylodes macrocephala Koidz. 15 - 25 parts, Bombyx batryticatus 9 - 15 parts, Cryptotympana pustulata Fabricius 9 - 15 parts, Vitex trifolia L. var. simplicifolia Cham. 10 - 20 parts, Pueraria lobata (Willd.) Ohwi 10 - 20 parts, Poria cocos (Schw.) Wolf 15 - 25 parts, Salvia miltiorrhiza Bunge 9 - 15 parts, Paeonia lactiflora Pall. 10 - 15 parts, Ligusticum wallichii Franch. 10 - 15 parts, Schizonepeta tenuifolia Briq. 10 - 15 parts, Scutellaria baicalensis Georgi 9 - 15 parts, Bupleurum chinense DC. 10 - 15 parts, Licorice Root 3 - 9 parts.

[0106] 18. Control Group 15: Application No. CN201310475339.2, A Traditional Chinese Medicine for Treating Headache of Kidney Deficiency and Blood Stasis Syndrome: Rehmannia glutinosa Libosch. 25 - 30 parts, Cornus officinalis Sieb. et Zucc. 15 - 20 parts, Poria cocos (Schw.) Wolf 25 - 30 parts, Alisma orientale (Sam.) Juz. 8 - 10 parts, Dioscorea opposita Thunb. 8 - 10 parts, Acorus gramineus Soland. 8 - 10 parts, Prunus persica (L.) Batsch 8 - 10 parts, Paeonia lactiflora Pall. 15 - 20 parts, Paeonia suffruticosa Andr. 8 - 10 parts, Os Draconis 12 - 15 parts, Concha Ostreae 25 - 30 parts, Cinnamomum cassia Presl 12 - 15 parts, Atractylodes macrocephala Koidz. 15 - 20 parts, Licorice Root 4 - 6 parts, Gastrodia elata Bl. 8 - 10 parts, Pinellia ternata (Thunb.) Breit. 8 - 10 parts, Ligusticum wallichii Franch. 25 - 30 parts, Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav. 8 - 10 parts, Pueraria lobata (Willd.) Ohwi 20 - 24 parts, Vitex trifolia L. var. simplicifolia Cham. 12 - 15 parts, Asarum sieboldii Miq. 4 - 6 parts, Coptis chinensis Franch. 4 - 6 parts, Bombyx batryticatus 8 - 10 parts, Carthamus tinctorius L. 8 - 10 parts, Scolopendra subspinipes mutilans L. Koch 1 piece, Eucommia ulmoides Oliv. 8 - 10 parts, Achyranthes bidentata Blume 12 - 15 parts, Viscum coloratum (Kom.) Nakai 12 - 15 parts, Eupolyphaga sinensis Walker 8 - 10 parts, Cornu Cervi Degelatinatum 3 - 5 parts, Epimedium brevicornu Maxim. 25 - 30 parts, Lycium barbarum L. 12 - 15 parts, Cinnamomum cassia Presl 8 - 10 parts.

[0107] 19. Control Group 16: Application No. CN201410444130.4, A Traditional Chinese Medicine Composition for Cervicogenic Headache and Its Preparation Method: Gypsum Fibrosum 25 g, Eriocaulon buergerianum Koern. 9 g, Indigo Naturalis 20 g, Bupleurum chinense DC. 15 g, Pinellia ternata (Thunb.) Breit. 12 g, Nelumbo nucifera Gaertn. 15 g, Gastrodia elata Bl. 6 g, Haematitum 15 g, Saposhnikovia divaricata (Turcz.) Schischk. 9 g, Buthus martensii Karsch 6 g, Paeonia lactiflora Pall. 15 g, Astragalus membranaceus (Fisch.) Bunge 25 g, Pheretima aspergillum (E. Perrier) 9 g, Poria cocos (Schw.) Wolf 15 g, Leonurus japonicus Houtt. 25 g, Eupatorium lindleyanum DC. 15 g, Carthamus tinctorius L. 9 g, Securinega suffruticosa (Pall.) Rehd. 9 g, Ligusticum sinense Oliv. 12 g, Prunella vulgaris L. 9 g, Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav. 12 g, Bombyx batryticatus 9 g, Angelica sinensis (Oliv.) Diels 15 g, Ligusticum wallichii Franch. 12 g, Paeonia lactiflora Pall. 12 g, Rosmarinus officinalis L. 9 g, Citrus reticulata Blanco 10 g, Pueraria lobata (Willd.) Ohwi 18 g, Ramulus Mori 25 g.

[0108] 20. Control group 17: Application number CN201010300445.3 A Chinese medicine composition for treating normal and migraine headaches and its preparation method: Chuanxiong 15g, Danshen 15g, Aucklandia 9g, Linglingxiang 1g, Benzoin 1g, Tangerine peel 9g, Curcuma 9g, Cyperus 9g, Coptis 8g, Scutellaria 12g, Cinnamon 8g, Notopterygium 9g, Angelica dahurica 10g, Pueraria lobata 15g, Peach kernel 8g, Carthamus 9g, Trigonosciadium 9g, Curcuma 9g, Squama 15g , raw astragalus 20g, white peony 12g, red peony 10g, Gastrodia 9g, Uncaria 12g, storax 15g, Acorus calamus 10g, Schizonepeta 9g, Saposhnikovia 9g, Ephedra root 8g, Forsythia suspensa 9g, Asarum 9g, Magnolia 9g, Mint 10g, raw Rehmannia glutinosa 20g, Toosendan fruit 12g, Rose 15g, Panax notoginseng 6g, Dalbergia odorifera 9g, Sanguisorba officinalis 12g, Leonurus japonicus 15g, Vaccaria segetalis 10g, Eupolyphaga sinensis 9g, Sappan wood 9g, Sanguisorba officinalis 8g, Pinellia 9g.

[0109] 21. Control group 18: Application No. CN200510016880.2 A medicine for treating headache: 15g of bupleurum, 12g of white peony root, 9g of immature bitter orange, 8g of liquorice, 15g of chuanxiong, 10g of rhubarb, 9g of cyperus rotundus, 15g of gypsum, 12g of scutellaria, 9g of gentian, 12g of Uncaria rhynchophylla, 9g of earthworm, 9g of bombyx batryticatus, 10g of angelica dahurica, 12g of vitex rotundus, 15g of chrysanthemum, and 15g of wolfberry.

[0110] 22. Control group 19: Application No. CN200410013510.9 Traditional Chinese medicine for treating tension-type headache: 10-20 parts of Chuanxiong, 8-12 parts of Bupleurum, 10-15 parts of Angelica dahurica, 10-12 parts of Cyperus rotundus, 2-5 parts of Asarum, 15-20 parts of Notopterygium wilfordii, 10-20 parts of White Peony Root, 10-20 parts of Prunella Vulgaris, 10-20 parts of Ziziphus jujuba seeds, 10-20 parts of Angelica sinensis, 25-35 parts of Puerariae Radix, and 8-12 parts of Citrus aurantium.

[0111] 23. Control group 20: Preparation method of traditional Chinese medicine for treating headache with authorization number CN100352486C: Corydalis yanhusuo 15g, Gastrodia elata 9g, Ligusticum chuanxiong 15g, Bombyx batryticatus 9g, Angelica dahurica 10g, Notopterygium incisum 9g, Pueraria root 15g, Achyranthes bidentata 15g, Bupleurum 15g.

[0112] 24. Control group 21: Authorization number CN100352485C, a method for preparing a traditional Chinese medicine for treating headache: Corydalis 15g, Gastrodia 9g, Ligusticum chuanxiong 15g, Bombyx batryticatus 9g, Angelica dahurica 10g, Notopterygium incisum 9g, Pueraria lobata 15g, Saposhnikovia divaricata 9g, Achyranthes bidentata 15g, Vitex rotundus 12g, Platycodon grandiflorum 12g.

[0113] 25. Control Group 22: Patent No. CN1102402C, a traditional Chinese medicine granule for treating headache and its preparation method: 600 - 1000 parts of Ligusticum chuanxiong, 100 - 400 parts of Paeonia lactiflora, 100 - 400 parts of Cyperus rotundus, 100 - 400 parts of Bupleurum chinense, 100 - 400 parts of Scutellaria baicalensis, 100 - 400 parts of Angelica dahurica, 100 - 400 parts of Pinellia ternata, 100 - 400 parts of Gastrodia elata, 300 - 500 parts of Corydalis yanhusuo, 100 - 400 parts of Corydalis decumbens.

[0114] 26. Control Group 23: Patent No. CN102178751B, a brain - clearing and pain - relieving preparation: 12 g of Paeonia lactiflora, 15 g of Ligusticum chuanxiong, 15 g of Pueraria lobata, 15 g of Corydalis yanhusuo, 9 g of Tribulus terrestris, 10 g of Angelica dahurica, 10 g of Acanthopanax senticosus, and 15 g of Corydalis decumbens.

[0115] 27. Control Group 24: Application No. CN201410443993.X, a pharmaceutical preparation for relieving headache caused by cervical spondylosis and its preparation method: 12 g of Haematitum, 10 g of Saposhnikovia divaricata, 4 g of Scorpio, 15 g of Paeonia lactiflora, 9 g of Artemisia anomala, 24 g of Pueraria lobata, 20 g of Astragalus membranaceus, 30 g of Leonurus japonicus, 15 g of Eupatorium japonicum, 8 g of Carthamus tinctorius, 12 g of Ligusticum sinense, 12 g of Prunella vulgaris, 12 g of Angelica dahurica, 30 g of Poria cocos, 8 g of Eriocaulon buergerianum, 15 g of Angelica sinensis, 12 g of Arisaema erubescens, 12 g of Ligusticum chuanxiong, 12 g of Paeonia lactiflora, 9 g of Citrus reticulata Blanco, 12 g of Alisma orientale, 30 g of Os draconis, 12 g of Prunus persica, 9 g of Notopterygium incisum, 10 g of Uncaria rhynchophylla, 18 g of Ziziphus jujuba Mill. var. spinosa, 12 g of Pinellia ternata, 9 g of Pheretima aspergillum, 30 g of Concha ostreae, 8 g of Glycyrrhiza uralensis, 6 g of Bombyx batryticatus.

[0116] 28. Control Group 25: Application No. CN202010482583.1, a traditional Chinese medicine composition for nursing headache after neurosurgery: 12 g of Paeonia lactiflora, 9 g of Scorpio, 9 g of Curcuma aromatica, 10 g of Angelica dahurica, 15 g of Ligusticum chuanxiong, 10 g of Paeonia lactiflora, 15 g of Salvia miltiorrhiza, 15 g of Prunella vulgaris, 12 g of Uncaria rhynchophylla, 9 g of Gastrodia elata, 8 g of Glycyrrhiza uralensis, 10 g of Aconitum carmichaelii, 9 g of Herba Speranskiae tuberculatae, 15 g of Angelica sinensis, 9 g of Arisaema heterophyllum, 9 g of Schefflera arboricola, 9 g of Saposhnikovia divaricata, 9 g of Notopterygium incisum, 9 g of Angelica pubescens, 15 g of Poria cocos, 8 g of Cinnamomum cassia, 15 g of Corydalis yanhusuo, 15 g of Leonurus japonicus, and 20 g of Carapax et Plastrum Testudinis.

[0117] 29. Control Group 26: Application No. CN201810552479.8, a traditional Chinese medicine pill for treating tension headache and its preparation method: 35 - 45 parts of Ligusticum chuanxiong, 30 - 35 parts of Angelica dahurica, 5 - 10 parts of Paeonia lactiflora, 5 - 10 parts of Gastrodia elata, 5 - 10 parts of Concha haliotidis, 5 - 10 parts of Evodia rutaecarpa, 3 - 5 parts of Schizonepeta tenuifolia, 3 - 5 parts of Corydalis yanhusuo, 3 - 5 parts of Ligusticum sinense, 3 - 5 parts of Ligustrum lucidum.

[0118] 30. Control Group 27: Application No. CN201810232464.3, an acupoint patch for treating headache: 10 - 20 parts of Ligusticum chuanxiong, 10 - 20 parts of Notopterygium incisum, 10 - 20 parts of Angelica dahurica, 10 - 20 parts of Paeonia lactiflora, 10 - 20 parts of Brassica alba, 10 - 15 parts of Prunus japonica, 10 - 15 parts of Evodia rutaecarpa, 10 - 15 parts of Mentha haplocalyx, 8 - 12 parts of Cyperus rotundus, 8 - 12 parts of Bupleurum chinense.

[0119] 31. Control Group 28: Application No. CN201810159749.9, a drug for treating vascular headache: 10 - 15 parts of Ligusticum chuanxiong, 12 - 15 parts of Gastrodia elata, 10 - 13 parts of Vitex trifolia var. simplicifolia, 10 - 20 parts of Paeonia lactiflora, 10 - 13 parts of Corydalis yanhusuo, 15 - 18 parts of Angelica dahurica, 12 - 15 parts of Salvia miltiorrhiza, 13 - 16 parts of Ligusticum sinense, 10 - 15 parts of Prunus persica, 8 - 12 parts of Carthamus tinctorius, 10 - 15 parts of Bupleurum chinense, 3 - 5 parts of Scorpio, 12 - 18 parts of Gardenia jasminoides, 2 - 4 parts of Scolopendra subspinipes, 10 - 13 parts of Curcuma zedoaria and honey.

[0120] 32. Control Group 29: Application No. CN201610909089.2, a drug for treating headache caused by cervical spondylosis: 5 g of Pueraria lobata, 12 g of Bupleurum chinense, 30 g of Paeonia lactiflora, 30 g of Ligusticum chuanxiong, 12 g of Angelica dahurica, 10 g of Cyperus rotundus, 10 g of Armeniaca vulgaris, 6 g of Asarum sieboldii, 20 g of Cornu Caprae seu Ovis (scraped), 6 g of Ligusticum chuanxiong, 3 g of Radix Aconiti Preparata, 6 g of Angelica dahurica, 20 g of Raphanus sativus.

[0121] 33. Control Group 30: Application No. CN201711102355.1, a capsule drug for treating headache and its preparation method: 6 g of Styrax, 60 g of Angelica dahurica, 80 g of Ligusticum chuanxiong, 80 g of Heracleum hemsleyanum, 12 g of Benzoin, 120 g of Rheum officinale, 50 g of Acorus tatarinowii, 120 g of Eupatorium fortunei, 80 g of Saposhnikovia divaricata, 110 g of Angelica sinensis, 150 g of Paeonia lactiflora, 90 g of Fructus Aurantii Immaturus, 80 g of Paeonia veitchii, 90 g of Saposhnikovia divaricata, 90 g of Cyperus rotundus, 120 g of Lignum Dalbergiae Odoriferae, 150 g of Notopterygium incisum, 40 g of Aquilaria sinensis, 200 g of Spatholobus suberectus, 120 g of Salvia miltiorrhiza, 120 g of Prunus persica, 60 g of Manis pentadactyla, 80 g of Carthamus tinctorius, 200 g of Leonurus japonicus, 60 g of Brassica alba.

[0122] 34. Control Group 31: Application No. CN201711109518.9, a decoction drug that can effectively treat headache and its preparation method: 3 g of Cornu Saigae Tataricae powder, 9 g of Gastrodia elata, 30 g of Smilax glabra, 8 g of Paeonia veitchii, 12 g of Uncaria rhynchophylla, 12 g of Paeonia lactiflora, 15 g of Achyranthes bidentata, 6 g of Scorpio, 10 g of Paeonia suffruticosa, 8 g of Ephedra sinica, 12 g of Ligusticum chuanxiong, 9 g of Leonurus japonicus, 15 g of Trichosanthes kirilowii, 12 g of Cassia obtusifolia, 3 g of Asarum sieboldii, 15 g of Angelica sinensis, 9 g of Saposhnikovia divaricata, 12 g of Atractylodes macrocephala, 20 g of Rheum officinale, 9 g of Aconitum carmichaelii, 9 g of Curcuma aromatica, 9 g of Angelica dahurica, 12 g of Schizonepeta tenuifolia, 40 g of Ipomoea batatas leaves, 20 g of Pueraria lobata, 12 g of Polygonatum sibiricum, 12 g of Anemarrhena asphodeloides, 8 g of Carthamus tinctorius.

[0123] 35. Control Group 32: Application No. CN201711037656.0, A New Traditional Chinese Medicine Composition for Treating Headache and Its Preparation Method: Uncaria rhynchophylla 200g, Ligusticum chuanxiong 90g, Pueraria lobata 120g, Ephedra sinica 75g, Asarum sieboldii 55g, Prepared Aconite Root 90g, Bupleurum chinense 90g, White Peony Root 110g, Notopterygium incisum 180g, Angelica pubescens 90g, Scutellaria baicalensis 65g, Saposhnikovia divaricata 85g, Rehmannia glutinosa 200g, Angelica sinensis 120g, Evodia rutaecarpa 35g, Spatholobus suberectus 130g, Prunus persica 120g, Carthamus tinctorius 80g, Angelica dahurica 75g, Ligusticum sinense 65g.

[0124] 36. Control Group 33: Application No. CN201710613494.4, A Capsule Drug for Treating Headache and Its Preparation Method: Uncaria rhynchophylla 270g, Chrysanthemum morifolium 80g, Ligusticum chuanxiong 95g, Ephedra sinica 80g, Ophiopogon japonicus (with removed heart) 115g, Asarum sieboldii 70g, Aconite Root 115g, Gastrodia elata 12g, White Peony Root 200g, Notopterygium incisum 200g, Cyathula officinalis 95g, Angelica pubescens 95g, Saposhnikovia divaricata 95g, Salvia miltiorrhiza 115g, Rehmannia glutinosa 290g, Angelica sinensis 115g, Curcuma aromatica 95g, Spatholobus suberectus 115g, Prunus persica 115g, Pueraria lobata 120g, Angelica dahurica 95g, Carthamus tinctorius 95g.

[0125] 37. Control Group 34: Application No. CN201611227581.8, A Health Food for Patients with Intractable Headache and Its Preparation Method: Notopterygium incisum 9g, White Peony Root 12g, Vitex trifolia 12g, Ligusticum chuanxiong 15g, Angelica dahurica 10g, Platycodon grandiflorum 9g, Cryptotympana pustulata 9g, Scutellaria baicalensis 12g, Pueraria lobata 15g, Crataegus pinnatifida 15g, Salvia miltiorrhiza 15g, Olibanum 9g, Tribulus terrestris 9g, Saposhnikovia divaricata 9g, Prunus persica 8g, Glycyrrhiza uralensis 8g.

[0126] 38. Control Group 35: Application No. CN201610558869.7, A Chinese Herbal Medicine Formula for Relieving Headache: Bupleurum chinense 25 - 35 parts; Angelica dahurica 25 - 35 parts; Cyperus rotundus 25 - 35 parts; White Peony Root 6 - 10 parts; Prunus japonica 6 - 10 parts; Brassica alba 2 - 8 parts; Glycyrrhiza uralensis 1 - 3 parts; Astragalus membranaceus 2 - 10 parts; Ligusticum chuanxiong 10 - 12 parts; Codonopsis pilosula 6 - 10 parts; Prunus persica 2 - 15 parts; Carthamus tinctorius 2#8 parts; Pheretima aspergillum 1 - 7 parts.

[0127] 39. Control Group 36: Application No. CN201610482716.9, A Traditional Chinese Medicine Composition for Treating Headache during Menstruation: Ligusticum chuanxiong 10#15 parts, Cyperus rotundus 3#8 parts, Rosa rugosa 12 - 18 parts, Eclipta prostrata 1 - 3 parts, Ligustrum lucidum 8 - 12 parts, Morus alba 13 - 18 parts, Corydalis yanhusuo 5 - 10 parts, Curcuma longa 1 - 5 parts, Lycopus lucidus 1 - 3 parts, Acanthopanax gracilistylus 5 - 10 parts, Cornus officinalis 3 - 8 parts, Trichosanthes kirilowii 8 - 13 parts, Anemarrhena asphodeloides 4 - 8 parts, Dry Ginger 8 - 12 parts, Artemisia argyi 4 - 8 parts, Polygala tenuifolia 1 - 3 parts, White Peony Root 3 - 8 parts, Acorus tatarinowii 1 - 5 parts, Polygonatum sibiricum 2 - 6 parts, Morinda officinalis 5 - 10 parts.

[0128] 40. Control group 37: Application No. CN201610084881.9, a medicinal wine for treating headache and its preparation method: 3 - 8 parts of asarum, 15 - 30 parts of adenophora root, 10 - 15 parts of vitex trifolia, 20 - 30 parts of chuanxiong rhizome, 10 - 15 parts of prunella vulgaris, 5 - 8 parts of phellodendron bark, 3 - 10 parts of dried ginger, 2 - 7 parts of prickly ash, 13 - 18 parts of gypsum fibrosum, 5 - 10 parts of roasted scorpio, 5 - 12 parts of bombyx batryticatus, 3 - 10 parts of salvia miltiorrhiza, 5 - 12 parts of white peony root, 3 - 8 parts of pseudostellaria heterophylla, 2 - 8 parts of astragalus membranaceus, 5 - 10 parts of kudzu root, 8 - 15 parts of gastrodia elata, 50 - 100 parts of glutinous rice, and an appropriate amount of yeast.

[0129] 41. Control group 38: Application No. CN201610132479.3, a traditional Chinese medicine compound for treating headache: 15 g of Corydalis yanhusuo, 10 g of Angelica dahurica, 15 g of Millettia speciosa Champ., 15 g of Chuanxiong rhizome, 12 g of Vitex trifolia, 9 g of Ligusticum sinense Oliv., 15 g of Cyathula officinalis Kuan, 15 g of Pueraria lobata (Willd.) Ohwi, 12 g of Scutellaria baicalensis Georgi, 9 g of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag., and 8 g of Licorice root.

[0130] 42. Control group 39: Application No. CN201510984670.6, a drug for treating headache and its preparation method: 9 g of Forsythia suspensa, 15 g of Lonicera japonica Thunb., 10 g of Angelica dahurica, 15 g of Hawthorn fruit, 12 g of Isatis indigotica Fortune, 12 g of Gardenia jasminoides Ellis, 20 g of Rehmannia glutinosa Libosch., 15 g of Polygonatum sibiricum Redoute, 9 g of Schisandra chinensis (Turcz.) Baill., 9 g of Lotus leaf, 15 g of Angelica sinensis (Oliv.) Diels, 15 g of Chuanxiong rhizome, 8 g of Coptis chinensis Franch., 15 g of Chrysanthemum morifolium Ramat., 15 g of Trichosanthes kirilowii Maxim., 15 g of Cryptotympana pustulata Fabricius, 15 g of Leonurus artemisia (Lour.) S. Y. Hu, 15 g of Prunella vulgaris, 12 g of Lycium barbarum L., 9 g of Evodia rutaecarpa (Juss.) Benth., 12 g of Vitex trifolia, 15 g of Corydalis yanhusuo, 20 g of Mulberry leaf, 10 g of Chinese date, 9 g of Xanthium sibiricum Patrin ex Widder, 9 g of Bombyx batryticatus, 15 g of Imperata cylindrica Beauv. var. major (Nees) C. E. Hubb., 9 g of Schizonepeta tenuifolia Briq., 8 g of Mentha haplocalyx Briq.

[0131] 43. Control group 40: Authorization No. CN103349736B, a traditional Chinese medicine composition for treating nervous headache: 4 - 6 of Gastrodia elata, 2 - 4 of Angelica sinensis (Oliv.) Diels, 8 - 10 of Cortex moutan, 4 - 6 of Perilla frutescens (L.) Britt., 4 - 6 of Trichosanthes kirilowii Maxim. pericarpium, 2 - 4 of Chrysanthemum morifolium Ramat., 10 - 12 of Uncaria rhynchophylla (Miq.) Jacks., 22 - 26 of Polygonum multiflorum Thunb. var. thomsonii (Wall.) Stew., 25 - 27 of Concha haliotidis, 8 - 10 of Fructus gardeniae, 9 - 11 of Scutellaria baicalensis Georgi, 8 - 10 of Whitmania pigra Whitman, 6 - 8 of Eucommia ulmoides Oliv., 22 - 24 of Mulberry leaf, 11 - 13 of Poria cocos Wolf., 9 - 11 of Rhodiola rosea L., 9 - 11 of Scorpio, 6 - 8 of Bupleurum chinense DC., 6 - 8 of Bombyx batryticatus, 8 - 10 of Chuanxiong rhizome, 8 - 10 of Angelica dahurica, 1 - 2 of Scolopendra subspinipes mutilans L. Koch, 2 - 4 of Pueraria lobata (Willd.) Ohwi, 2 - 4 of Semen ziziphi spinosae, 15 - 18 of Clerodendrum philippinum Schauer leaves, 12 - 14 of Mentha haplocalyx Briq., 12 - 14 of Cornu caprae seu bubali powder.

[0132] 44. Control Group 41: Application No. CN201510478130.0, a pharmaceutical preparation for treating headache: 15 parts of Red Begonia Herb, 18 parts of Litsea mollis Hance, 5 parts of Pinellia ternata, 10 parts of White Peony Root, 30 parts of Atractylodes macrocephala, 10 parts of Astragalus membranaceus, 10 parts of Pseudostellaria heterophylla, 10 parts of Angelica sinensis, 5 parts of Licorice Root, 10 parts of Poria cum Radix Pini, 10 parts of Poria cocos, 10 parts of Polygala tenuifolia, 15 parts of Ziziphus jujuba var. spinosa, 15 parts of Arillus Longan, 5 parts of Aucklandia lappa, 20 parts of Albizia julibrissin, 15 parts of Germinated Barley, 10 parts of Lotus Leaf, 6 parts of Macrocarpium officinale, 10 parts of Pueraria lobata, 10 parts of Lycium barbarum, 5 parts of Angelica dahurica, 10 parts of Saposhnikovia divaricata, 5 parts of Hirudo, 5 parts of Ligusticum wallichii, 10 parts of Corydalis yanhusuo.

[0133] 45. Control Group 42: Application No. CN201510389673.5, a traditional Chinese medicine preparation for treating intractable headache and its preparation method: 9 g of Notopterygium incisum, 12 g of Scutellaria baicalensis, 12 g of White Peony Root, 15 g of Ligusticum wallichii, 15 g of Platycodon grandiflorum, 9 g of Cryptotympana pustulata Fabricius, 15 g of Pueraria lobata, 9 g of Arisaema heterophyllum Blume, 9 g of Gastrodia elata, 9 g of Olibanum, 12 g of Tribulus terrestris, 12 g of Vitex trifolia var. simplicifolia Cham., 8 g of Coptis chinensis, 9 g of Scolopendra subspinipes mutilans, 9 g of Asarum sieboldii Miq., 9 g of Scorpio, 9 g of Bombyx batryticatus, 8 g of Licorice Root.

[0134] 46. Control Group 43: Authorization No. CN103301324B, a traditional Chinese medicine for treating neural headache: 0 - 25 g of Ligusticum wallichii, 0 - 35 g of Angelica dahurica, 0 - 40 g of Artemisia argyi, 0 - 20 g of Gastrodia elata, 0 - 15 g of Scolopendra subspinipes mutilans, 0 - 35 g of Uncaria macrophylla Wall., 0 - 25 g of Pueraria lobata, 0 - 20 g of Corydalis yanhusuo, 0 - 20 g of Vitex trifolia var. simplicifolia Cham.

[0135] 47. Control Group 44: Application No. CN201410369117.7, a Chuanxiong Qingnao Zhentong Capsule and its preparation method: 25% of Ligusticum wallichii, 10% - 12% of Gastrodia elata, 8% - 10% of Asarum sieboldii Miq., 10% - 12% of Angelica dahurica, 10% - 12% of Panax notoginseng, 13% - 15% of Pueraria lobata, 18% - 20% of White Peony Root.

[0136] 48. Control Group 45: Application No. CN201410194934.3, a traditional Chinese medicine composition for treating neural headache: 10 g of Radix Aconiti Lateralis Preparata, 25 g of Ligusticum wallichii, 30 g of Angelica dahurica, 6 g of Asarum sieboldii Miq., 6 g of Scorpio, 2 pieces of Scolopendra subspinipes mutilans, 15 g of Gastrodia elata, 10 g of Bombyx batryticatus, 30 g of White Peony Root, 10 g of Radix Glycyrrhizae Preparata, 30 g of Tribulus terrestris, 30 g of Magnetitum, 30 g of Concha Margaritifera Usta, 15 g of Curcuma aromatica Salisb., 30 g of Salvia miltiorrhiza, 15 g of Ziziphus jujuba var. spinosa, 10 g of Achyranthes bidentata, 30 g of Caulis Polygoni Multiflori, 10 g of Spica Schizonepetae.

[0137] 49. Control Group 46: Application No. CN201410139058.4, a traditional Chinese medicine preparation for treating angioneurotic headache: 10 - 15 grams of Ligusticum chuanxiong, 10 - 15 grams of Ligusticum sinense, 10 - 15 grams of Angelica dahurica, 10 - 15 grams of Vitex trifolia var. simplicifolia, 8 - 12 grams of Saposhnikovia divaricata, 8 - 12 grams of Notopterygium incisum, 12 - 18 grams of Pueraria lobata, 12 - 18 grams of Bupleurum chinense, 12 - 18 grams of Paeonia lactiflora, 10 - 15 grams of Scutellaria baicalensis, 7 - 12 grams of Pinellia ternata, 10 - 15 grams of Salvia miltiorrhiza, 5 - 8 grams of Carthamus tinctorius, 10 - 15 grams of Angelica sinensis, 5 - 7 grams of Glycyrrhiza uralensis.

[0138] 50. Control Group 47: Authorization No. CN103223122B, a traditional Chinese medicine for treating intractable headache: 20 - 30 g of Ligusticum chuanxiong, 10 - 15 g of Angelica dahurica, 6 - 10 g of prepared Cyperus rotundus, 20 - 30 g of Paeonia lactiflora, 10 - 15 g of Gastrodia elata, 10 - 15 g of Bombyx batryticatus, 10 - 15 g of Angelica sinensis, 5 - 10 g of Asarum sieboldii, 6 - 10 g of Saposhnikovia divaricata, and 6 - 10 g of Pheretima aspergillum.

[0139] 51. Control Group 48: Application No. CN201310236595.6, a traditional Chinese medicine composition for treating neural headache: 4 - 6 of Gastrodia elata, 2 - 4 of Angelica sinensis, 8 - 10 of Cortex moutan, 4 - 6 of Perilla frutescens, 4 - 6 of Fructus trichosanthis peel, 2 - 4 of Flos chrysanthemi, 10 - 12 of Uncaria rhynchophylla, 22 - 26 of Caulis polygonati multiflori, 25 - 27 of Concha haliotidis, 8 - 10 of stir - fried Fructus gardeniae, 9 - 11 of Scutellaria baicalensis, 8 - 10 of Hirudo, 6 - 8 of Eucommia ulmoides, 22 - 24 of Folium mori, 11 - 13 of Poria cumradix pini, 9 - 11 of Rhodiola rosea, 9 - 11 of Scorpio, 6 - 8 of Bupleurum chinense, 6 - 8 of Bombyx batryticatus, 8 - 10 of Ligusticum chuanxiong, 8 - 10 of Angelica dahurica, 1 - 2 of Scolopendra subspinipes, 2 - 4 of Pueraria lobata, 2 - 4 of Semen ziziphi spinosae, 15 - 18 of Clerodendrum philippinum leaves, 12 - 14 of Mentha haplocalyx, 12 - 14 of Cornu caprae seu ovis powder.

[0140] 52. Control Group 49: Application No. CN201310312125.3, a traditional Chinese medicine composition for treating headache and its preparation method: 15 g of Ligusticum chuanxiong, 9 g of Gastrodia elata, 10 g of Angelica dahurica, 12 g of Paeonia lactiflora, 15 g of Corydalis yanhusuo, and 9 g of Scorpio.

[0141] 53. Control Group 50: Application No. CN201110408945.3, a traditional Chinese medicine for treating headache: 28 - 33 g of Bupleurum chinense; 18 - 22 g of Scutellaria baicalensis; 28 - 33 g of Pueraria lobata; 18 - 22 g of Ligusticum chuanxiong; 18 - 22 g of Angelica dahurica; 28 - 32 g of Flos chrysanthemi; 28 - 32 g of Paeonia lactiflora; 18 - 22 g of Poria cocos; 12 - 17 g of Mentha haplocalyx; 12 - 17 g of Saposhnikovia divaricata; 3 - 7 g of Glycyrrhiza uralensis.

[0142] Group 51 control: Application No. CN201210114310.7, a traditional Chinese medicine composition for treating headache and its preparation method: 20 g of Smilax glabra, 12 g of antelope horn powder, 9 g of Gastrodia elata, 10 g of Radix Paeoniae Rubra and Alba, 15 g of Achyranthes bidentata, 15 g of Cortex Moutan, 9 g of Scorpio, 15 g of Ligusticum chuanxiong, 9 g of Leonurus japonicus, 15 g of Cassia obtusifolia, 15 g of Trichosanthes kirilowii, 15 g of Angelica sinensis, 9 g of Saposhnikovia divaricata, 15 g of Rheum officinale, 12 g of Atractylodes macrocephala, 9 g of Curcuma aromatica, 10 g of Angelica dahurica, 10 g of Ipomoea batatas leaves, 9 g of Schizonepeta tenuifolia, 15 g of Pueraria lobata, 15 g of Polygonatum sibiricum and 12 g of Anemarrhena asphodeloides.

[0143] Group 52 control: Authorization No. CN102139064B, a traditional Chinese patent medicine for preventing and treating vascular neurogenic headache: 10 - 20 of Atractylodes macrocephala, 3 - 6 of Angelica dahurica, 10 - 15 of Gastrodia elata, 5 - 15 of Ephedra sinica, 10 - 20 of Ligusticum chuanxiong, 10 - 20 of Uncaria rhynchophylla, 20 - 30 of Corydalis yanhusuo, 20 - 30 of Vitex trifolia var. simplicifolia, 20 - 30 of Pueraria lobata.

[0144] Group 53 control: Authorization No. CN101829315B, a traditional Chinese medicine composition for treating menstrual headache and its preparation method: 10 g of Acorus tatarinowii, 9 g of Benzoin, 9 g of Pogostemon cablin, 15 g of Ligusticum chuanxiong, 8 g of Cinnamomum cassia, 15 g of Salvia miltiorrhiza, 9 g of Styrax, 9 g of Aurantii Fructus Immaturus, 9 g of Saposhnikovia divaricata, 9 g of Persica vulgaris, 9 g of Carthamus tinctorius, 9 g of Citrus reticulata Blanco, 9 g of Citrus reticulata Blanco var. austera, 15 g of Melia toosendan, 10 g of Angelica dahurica, 9 g of Notopterygium incisum, 9 g of Gastrodia elata, 6 g of Panax notoginseng, 9 g of Mentha haplocalyx Briq., 8 g of Zingiber officinale, 9 g of Piper longum, 9 g of Aquilaria sinensis (Lour.) Spreng., 9 g of Cyperus rotundus, 12 g of Curcuma aromatica, 15 g of Corydalis yanhusuo. Group 54 control: Application No. CN201110151218.3, a medicine for treating headache and its preparation method

[0145] Group 57 treatment: A medicine for treating headache and its preparation method: 15 g of Chrysanthemum morifolium, 15 g of Bupleurum chinense, 15 g of Ligusticum chuanxiong, 20 g of Astragalus membranaceus, 15 g of Angelica sinensis, 10 g of Tribulus terrestris, 9 g of Lycium barbarum, 9 g of Bombyx batryticatus, 12 g of Radix Paeoniae Alba, 8 g of Glycyrrhiza uralensis, 9 g of Scolopendra subspinipes mutilans, 15 g of Pueraria lobata, 9 g of Scolopendra subspinipes mutilans.

[0146] Group 55 control: Application No. CN201110075770.9, a traditional Chinese patent medicine for preventing and treating vascular neurogenic headache: 10 - 20 of Atractylodes macrocephala, 3 - 6 of Angelica dahurica, 10 - 15 of Gastrodia elata, 5 - 15 of Ephedra sinica, 10 - 20 of Ligusticum chuanxiong, 10 - 20 of Uncaria rhynchophylla, 20 - 30 of Corydalis yanhusuo, 20 - 30 of Vitex trifolia var. simplicifolia, 20 - 30 of Pueraria lobata.

[0147] Group 56 control: Application No. CN201110073417.7, a drug for treating nervous headache: 10 - 15 parts of Ligusticum sinense, 10 - 15 parts of Vitex trifolia, 8 - 12 parts of Angelica dahurica, 10 - 15 parts of Ligusticum chuanxiong, 10 - 15 parts of Paeonia lactiflora Pall., 10 - 20 parts of Paeonia veitchii Lynch, 10 - 30 parts of Angelica sinensis, 10 - 30 parts of Salvia miltiorrhiza, 10 - 30 parts of Poria cum Radice Pini, 10 - 20 parts of Uncaria rhynchophylla, 10 - 20 parts of Scrophularia ningpoensis Hemsl., 8 - 15 parts of Gastrodia elata Blume, 5 - 10 parts of Pheretima aspergillum, 10 - 15 parts of Corydalis yanhusuo W. T. Wang, 10 - 30 parts of Concha Margaritifera Usta, 3 - 5 parts of Glycyrrhiza uralensis Fisch.

[0148] Group 57 control: Application No. CN201010030182.9, a traditional Chinese medicine capsule for treating headache: 12 g of Paeonia lactiflora Pall., 9 g of Cyperus rotundus L., 12 g of Scutellaria baicalensis Georgi, 8 g of Glycyrrhiza uralensis Fisch., 9 g of Mentha haplocalyx Briq., 15 g of Bupleurum chinense DC., 15 g of Chrysanthemi Flos, 15 g of Ligusticum chuanxiong Hort., 15 g of Poria cocos (Schw.) Wolf, 15 g of Angelica sinensis (Oliv.) Diels, 10 g of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 15 g of Moutan Cortex.

[0149] Group 58 control: Application No. CN201010530759.2, a drug for treating vascular headache and its preparation method: 12 - 15 parts of Ligusticum chuanxiong Hort., 12 - 15 parts of Gastrodia elata Blume, 10 - 13 parts of Vitex trifolia L., 15 - 18 parts of Paeonia lactiflora Pall., 10 - 13 parts of Corydalis yanhusuo W. T. Wang, 15 - 18 parts of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 12 - 15 parts of Salvia miltiorrhiza Bunge, 13 - 16 parts of Ligusticum sinense Oliv., 9 - 12 parts of Bupleurum chinense DC., 2 - 5 parts of Scolopendra subspinipes mutilans L. Koch, 13 - 16 parts of Gardenia jasminoides Ellis, 10 - 13 parts of Rhizoma Curcumae, 3 - 5 parts of Glycyrrhiza uralensis Fisch.

[0150] Group 59 control: Application No. CN201010215103.1, a traditional Chinese medicine composition for treating menstrual headache and its preparation method: 10 g of Acorus tatarinowii Schott, 9 g of Benzoin, 9 g of Pogostemon cablin (Blanco) Benth., 15 g of Ligusticum chuanxiong Hort., 8 g of Cinnamomum cassia Presl, 15 g of Salvia miltiorrhiza Bunge, 9 g of Styrax tonkinensis (Pierre) Craib ex Hartwich, 9 g of Aurantii Fructus Immaturus, 9 g of Saposhnikovia divaricata (Turcz.) Schischk., 9 g of Persicae Semen, 9 g of Carthami Flos, 9 g of Citri Reticulatae Pericarpium, 9 g of Citri Reticulatae Viride Pericarpium, 9 g of Toosendan Fructus, 10 g of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 9 g of Notopterygium incisum Ting ex Hsiao et K. C. Hsia, 9 g of Gastrodia elata Blume, 9 g of Panax notoginseng (Burkill) F. H. Chen ex C. Hoo & Tsai, 9 g of Mentha haplocalyx Briq., 8 g of Zingiber officinale Rosc., 9 g of Piper longum L., 9 g of Aquilariae Lignum Resinatum, 9 g of Cyperus rotundus L., 9 g of Curcumae Radix, 15 g of Corydalis yanhusuo W. T. Wang.

[0151] Group 60 control: Application No. CN201010150031.7, a traditional Chinese medicine composition for treating headache and its preparation method: 10 g of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 15 g of Ligusticum chuanxiong Hort., 15 g of Styrax tonkinensis (Pierre) Craib ex Hartwich, 9 g of Benzoin, 10 g of Acorus tatarinowii Schott, 9 g of Pogostemon cablin (Blanco) Benth., 8 g of Rheum officinale Baill., 15 g of Angelica sinensis (Oliv.) Diels, 12 g of Paeonia lactiflora Pall., 10 g of Paeonia veitchii Lynch, 9 g of Aurantii Fructus Immaturus, 9 g of Cyperus rotundus L., 9 g of Notopterygium incisum Ting ex Hsiao et K. C. Hsia, 9 g of Aquilariae Lignum Resinatum, 9 g of Lignum Dalbergiae Odoriferae, 15 g of Salvia miltiorrhiza Bunge, 9 g of Persicae Semen, 9 g of Carthami Flos, 15 g of Leonuri Herba, 12 g of Manis pentadactyla L.

[0152] Group 61 of the control: A Fangfeng Duzhong compound decoction for treating headache, application number CN200910015081.1: 9g of Saposhnikovia divaricata, 9g of Eucommia ulmoides, 15g of Ligusticum chuanxiong, 15g of Spatholobus suberectus, 15g of Angelica sinensis, 9g of Taxillus chinensis, 15g of Cassia obtusifolia, 9g of Schizonepeta tenuifolia, 9g of Gastrodia elata, 10g of Angelica dahurica, 9g of Asarum sieboldii, 9g of Scorpio, 10g of Mentha haplocalyx, 15g of Corydalis yanhusuo, 9g of Cyperus rotundus.

[0153] Group 62 of the control: Soft capsules for treating vascular headache, authorization number CN100534482C: 15g of Ligusticum chuanxiong, 15g of Bupleurum chinense, 10g of Angelica dahurica, 9g of Semen sinapis albae, 12g of Paeonia lactiflora, 9g of Cyperus rotundus, 9g of Prunus japonica, 8g of Glycyrrhiza uralensis.

[0154] Group 63 of the control: A traditional Chinese medicine water pill formula for treating headache, application number CN201810868194.5: 15g of Ligusticum chuanxiong, 9g of Mentha haplocalyx, 9g of Kaempferia galanga, 10g of Angelica dahurica, 9g of Asarum sieboldii, 9g of Schizonepeta tenuifolia, 9g of Saposhnikovia divaricata, 9g of Notopterygium incisum, 9g of Ligusticum sinense, 12g of Vitex trifolia var. simplicifolia, 9g of Xanthium sibiricum, 15g of Pueraria lobata, 12g of Gardenia jasminoides, 15g of Sojae Semen Preparatum, 9g of Forsythia suspensa, 12g of Chrysanthemum morifolium, 12g of Scutellaria baicalensis Georgi stir-fried, 15g of Platycodon grandiflorum, 9g of Oroxylum indicum, 9g of Medicated Leaven, 9g of Raphanus sativus L. stir-fried, 8g of Zingiber officinale, 8g of Glycyrrhiza uralensis.

[0155] Group 64 of the control: A traditional Chinese medicine for treating migraine, application number CN201410511888.5: 15g of Ligusticum chuanxiong, 9g of Asarum sieboldii, 15g of Salvia miltiorrhiza, 10g of Angelica dahurica, 15g of Pueraria lobata, 9g of Scorpio, 15g of Polygonum multiflorum Thunb, 9g of Cyperus rotundus, 12g of Gardenia jasminoides, 12g of Atractylodes lancea, 15g of Angelica sinensis, 20g of Smilax glabra, 9g of Scolopendra subspinipes, 12g of Paeonia lactiflora, 20g of Rehmannia glutinosa, 8g of Coptis chinensis, 9g of Saposhnikovia divaricata.

[0156] Group 65 of the control: A traditional Chinese medicine formula for treating headache, application number CN201510820375.7: 30g of Ligusticum chuanxiong, 15g of Paeonia lactiflora, 15g of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chow, 15g of Pueraria lobata, 10g of Gastrodia elata, 10g of Bombyx batryticatus, 3g of Semen sinapis albae, 3g of Asarum sieboldii.

[0157] Group 66 of the control: A traditional Chinese medicine for treating vascular and nervous headache, application number CN201410479470.0: 9g of Schizonepeta tenuifolia, 9g of Saposhnikovia divaricata, 10g of Angelica dahurica, 9g of Asarum sieboldii, 15g of Chrysanthemum morifolium, 12g of Vitex trifolia var. simplicifolia, 15g of Angelica sinensis, 15g of Ligusticum chuanxiong, 12g of Paeonia lactiflora, 15g of Bupleurum chinense, 12g of Scutellaria baicalensis Georgi, 15g of Achyranthes bidentata, 9g of Pheretima aspergillum, 9g of Scorpio, 9g of Bombyx batryticatus.

[0158] Control Group 67: Application No. CN201410485437.9, a traditional Chinese medicine composition for treating nervous headache: 10 - 20 parts of chrysanthemum, 10 - 20 parts of Corydalis yanhusuo, 15 - 25 parts of hematite, 15 - 25 parts of Tribulus terrestris, 10 - 15 parts of Uncaria rhynchophylla, 25 - 35 parts of nacre, 15 - 25 parts of Atractylodes macrocephala, 9 - 15 parts of Bombyx batryticatus, 9 - 15 parts of Cryptotympana pustulata, 10 - 20 parts of Vitex trifolia, 10 - 20 parts of Pueraria lobata, 15 - 25 parts of Poria cocos, 9 - 15 parts of Salvia miltiorrhiza, 10 - 15 parts of Paeonia lactiflora, 10 - 15 parts of Ligusticum chuanxiong, 10 - 15 parts of Schizonepeta tenuifolia, 9 - 15 parts of Scutellaria baicalensis, 10 - 15 parts of Bupleurum chinense, 3 - 9 parts of Glycyrrhiza uralensis.

[0159] Control Group 68: Application No. CN201310602505.0, a traditional Chinese medicine composition for treating severe headache and its preparation method: 12 grams of Bupleurum chinense, 10 grams of Cyperus rotundus, 10 grams of Cinnamomum cassia, 10 grams of Citrus reticulata Blanco, 10 grams of Paeonia suffruticosa Andr., 10 grams of Paeonia lactiflora, 10 grams of Glycyrrhiza uralensis, 10 grams of Atractylodes macrocephala, 10 grams of Rehmannia glutinosa Libosch., 10 grams of Poria cocos, 10 grams of Armeniaca vulgaris Lam., 10 grams of Polygonum multiflorum Thunb., 10 grams of Pinellia ternata (Thunb.) Breit., 30 grams of Ligusticum chuanxiong, 30 grams of Angelica sinensis (Oliv.) Diels, 15 grams of Asarum sieboldii Miq., 15 grams of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 20 grams of Notopterygium incisum Ting ex Hsiao et K. C. Hsia, 10 grams of Vitex trifolia.

[0160] Control Group 69: Application No. CN201310475339.2, a traditional Chinese medicine for treating headache of kidney deficiency and blood stasis syndrome: 25 - 30 parts of Rehmannia glutinosa Libosch., 15 - 20 parts of Cornus officinalis Sieb. et Zucc., 25 - 30 parts of Poria cocos, 8 - 10 parts of Alisma orientale (Sam.) Juz., 8 - 10 parts of Dioscorea opposita Thunb., 8 - 10 parts of Acorus gramineus Soland., 8 - 10 parts of Prunus persica (L.) Batsch, 15 - 20 parts of Paeonia lactiflora, 8 - 10 parts of Paeonia suffruticosa Andr., 12 - 15 parts of Os draconis, 25 - 30 parts of Concha Ostreae, 12 - 15 parts of Cinnamomum cassia, 15 - 20 parts of Atractylodes macrocephala, 4 - 6 parts of Glycyrrhiza uralensis, 8 - 10 parts of Gastrodia elata Blume, 8 - 10 parts of Pinellia ternata (Thunb.) Breit., 25 - 30 parts of Ligusticum chuanxiong, 8 - 10 parts of Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav., 20 - 24 parts of Pueraria lobata, 12 - 15 parts of Vitex trifolia, 4 - 6 parts of Asarum sieboldii Miq., 4 - 6 parts of Coptis chinensis Franch., 8 - 10 parts of Bombyx batryticatus, 8 - 10 parts of Carthamus tinctorius L., 1 piece of Scolopendra subspinipes mutilans L. Koch, 8 - 10 parts of Eucommia ulmoides Oliv., 12 - 15 parts of Achyranthes bidentata Blume, 12 - 15 parts of Viscum coloratum (Kom.) Nakai, 8 - 10 parts of Eupolyphaga seu Steleophaga, 3 - 5 parts of Cornu Cervi Degelatinatum, 25 - 30 parts of Epimedium brevicornu Maxim., 12 - 15 parts of Lycium barbarum L., 8 - 10 parts of Cinnamomum cassia Presl.

[0161] Control Group 70: Chinese patent medicine preparation for treating nervous headache, application number CN201310369327.1: Cornu Saigae Tataricae 3 - 5 parts, Gastrodia elata 4 - 6 parts, Ligusticum wallichii 4 - 6 parts, Bupleurum chinense 4 - 6 parts, Cyperus rotundus 3 - 5 parts, Aurantii Fructus Immaturus 3 - 5 parts, Ziziphus jujuba var. spinosa 4 - 6 parts, Paeonia lactiflora Pall. 3 - 5 parts, Paeonia veitchii Lynch 3 - 5 parts, Angelica sinensis 3 - 5 parts, Os Draconis 6 - 8 parts, Concha Haliotidis 6 - 8 parts, Acorus calamus 3 - 5 parts, Curcuma aromatica Salisb. 3 - 5 parts, Asarum sieboldii Miq. 0.5 - 1.5 parts, Poria cocos 3 - 5 parts, Atractylodes macrocephala Koidz. 3 - 5 parts, Vitex trifolia var. simplicifolia Cham. 3 - 5 parts, Angelica dahurica (Fisch.) Benth. et Hook. f. 3 - 5 parts, Ligusticum sinense Oliv. 3 - 5 parts, Scorpio 1 - 3 parts, Scolopendra subspinipes mutilans L. Koch 0.5 - 1.5 parts, Pheretima aspergillum (E. Perrier) 3 - 5 parts, Amomum villosum Lour. 3 - 5 parts, Semen Myristicae 3 - 5 parts, Massa Fermentata Medica 3 - 5 parts, Rehmannia glutinosa Libosch. 4 - 6 parts, Colla Plastri Testudinis 6 - 8 parts, Colla Corii Asini 6 - 8 parts, Yellow rice wine 14 - 18 parts, Rock sugar 14 - 18 parts.

[0162] Control Group 71: Chinese patent medicine preparation for treating nervous headache, application number CN201210056942.2: Paeonia lactiflora Pall. 10 - 50 parts, Cicada slough 6 - 30 parts, Angelica dahurica (Fisch.) Benth. et Hook. f. 6 - 30 parts, Pueraria lobata (Willd.) Ohwi 10 - 40 parts, Cinnamomum cassia Presl 6 - 20 parts, Asarum sieboldii Miq. 2 - 6 parts, Ligusticum wallichii 5 - 20 parts, Vitex trifolia var. simplicifolia Cham. 5 - 30 parts, Glycyrrhiza uralensis Fisch. 4 - 20 parts.

[0163] Control Group 72: Chinese patent medicine for treating migraine - Xifeng Zhitong Granules and its preparation method, application number CN201210061770.8: Angelica sinensis 15g, Ligusticum wallichii 15g, Paeonia lactiflora Pall. 12g, Gastrodia elata 9g, Angelica dahurica (Fisch.) Benth. et Hook. f. 10g, Cynanchum paniculatum (Bunge) Kitag. 9g, Corydalis yanhusuo W. T. Wang 15g, Cyperus rotundus 9g.

[0164] Control Group 73: Chinese medicine composition for treating nervous headache, authorization number CN101804108B: Angelica sinensis 10 - 20g, Salvia miltiorrhiza 15 - 40g, Prunus persica (L.) Batsch 5 - 18g, Carthamus tinctorius L. 3 - 15g, Paeonia lactiflora Pall. 20 - 50g, Glycyrrhiza uralensis Fisch. 3 - 10g, Trogopterus dungarvanus (S. G. Gmelin) 5 - 15g, Myrrha 5 - 18g, Corydalis yanhusuo W. T. Wang 15 - 40g, Ligusticum wallichii 5 - 25g, Angelica dahurica (Fisch.) Benth. et Hook. f. 3 - 18g, Scutellaria baicalensis Georgi 5 - 20g, Chrysanthemi Flos 10 - 30g, Notopterygium incisum Ting ex H. T. Chang 10 - 25g.

[0165] Group 74 control: Patent No. CN101850097B, a traditional Chinese medicine composition for treating positive and migraine headaches and its preparation method: 15g of Ligusticum wallichii, 15g of Salvia miltiorrhiza, 9g of Aucklandia lappa, 9g of Dipteryx odorata, 9g of Benzoin, 9g of Citrus reticulata Blanco, 9g of Curcuma aromatica Salisb., 12g of Cyperus rotundus L., 8g of Coptis chinensis Franch., 12g of Scutellaria baicalensis Georgi, 8g of Cinnamomum cassia Presl, 9g of Notopterygium incisum Ting ex Hsiao & K. C. Hsia, 10g of Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., 15g of Pueraria lobata (Willd.) Ohwi, 9g of Prunus persica (L.) Batsch, 9g of Carthamus tinctorius L., 9g of Sparganium stoloniferum Buch.-Ham., 9g of Curcuma zedoaria (Christm.) Rosc., 9g of Manis pentadactyla Linnaeus, 20g of Astragalus membranaceus (Fisch.) Bunge, 12g of Paeonia lactiflora Pall., 10g of Paeonia veitchii Lynch, 9g of Gastrodia elata Blume, 12g of Uncaria rhynchophylla (Miq.) Miq. ex Havil., 9g of Styrax tonkinensis (Pierre) Craib ex Hartwich, 10g of Acorus tatarinowii Schott, 9g of Schizonepeta tenuifolia Briq., 9g of Saposhnikovia divaricata (Turcz.) Schischk., 8g of Radix licheniformis, 9g of Forsythia suspensa (Thunb.) Vahl, 9g of Asarum sieboldii Miq., 15g of Magnolia liliflora Desr., 10g of Mentha haplocalyx Briq., 20g of Rehmannia glutinosa (Gaertn.) DC., 15g of Melia toosendan Sieb. & Zucc., 15g of Rosa rugosa Thunb., 6g of Panax notoginseng (Burkill) F. H. Chen ex C. H. Chow, 9g of Dalbergia odorifera T. Chen, 15g of Sanguisorba officinalis L., 15g of Leonurus japonicus Houtt., 9g of Vaccaria segetalis (Neck.) Garcke, 9g of Eupolyphaga sinensis Walker, 10g of Caesalpinia sappan L., 9g of Sanguis draxonis, 9g of Pinellia ternata (Thunb.) Breit.

[0166] Group 75 control: Patent No. CN101396490B, a traditional Chinese medicine for treating neurovascular headache: 30 - 40g of Pueraria lobata (Willd.) Ohwi, 18 - 20g of Ligusticum wallichii, 18 - 20g of Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., 15 - 18g of Chrysanthemum morifolium Ramat., 10 - 15g of Corydalis yanhusuo W. T. Wang, 20 - 30g of Uncaria rhynchophylla (Miq.) Miq. ex Havil., 15 - 20g of Achyranthes bidentata Blume, 3 - 6g of Asarum sieboldii Miq., 10 - 15g of Scutellaria baicalensis Georgi, 10 - 15g of Ligusticum sinense Oliv., 10 - 15g of Alisma orientale (Sam.) Juz.

[0167] Group 76 control: Application No. CN200910019186.4, a traditional Chinese medicine composition for treating neural headache: 10 - 20g of Angelica sinensis (Oliv.) Diels, 15 - 40g of Salvia miltiorrhiza Bunge, 5 - 18g of Prunus persica (L.) Batsch, 3 - 15g of Carthamus tinctorius L., 20 - 50g of Paeonia lactiflora Pall., 3 - 10g of Glycyrrhiza uralensis Fisch., 5 - 15g of Trogopterus dungarvanus (P. Geoffroy), 5 - 18g of Myrrha, 15 - 40g of Corydalis yanhusuo W. T. Wang, 5 - 25g of Ligusticum wallichii, 3 - 18g of Angelica dahurica (Fisch. ex Hoffm.) Benth. & Hook. f. ex Franch. & Sav., 5 - 20g of Scutellaria baicalensis Georgi, 10 - 30g of Chrysanthemum morifolium Ramat., 10 - 25g of Notopterygium incisum Ting ex Hsiao & K. C. Hsia

[0168] Group 77 control: Application No. CN200910172546.4, Thrombolytic and Lipid - Lowering Pills: 15g of Bupleurum chinense DC., 15g of Pueraria lobata (Willd.) Ohwi, 15g of Salvia miltiorrhiza Bunge, 20g of Astragalus membranaceus (Fisch.) Bunge, 8g of Cinnamomum cassia Presl, 15g of Paeonia suffruticosa Andr., 12g of Paeonia lactiflora Pall., 10g of Paeonia veitchii Lynch, 15g of Angelica sinensis (Oliv.) Diels, 15g of Ligusticum wallichii (fried), 9g of Pheretima aspergillum (E. Perrier), 15g of Cassia obtusifolia L., 9g of Scorpio, 9g of Bombyx batryticatus, 9g of Scolopendra subspinipes mutilans L. Koch, 1g of Benzoin, 9g of Gastrodia elata Blume, 15g of Pseudostellaria heterophylla (Miq.) Pax ex Pax & Hoffm., 15g of Achyranthes bidentata Blume, 9g of Manis pentadactyla Linnaeus, 9g of Notopterygium incisum Ting ex Hsiao & K. C. Hsia, 9g of Typhonium giganteum Engl., 9g of Angelica pubescens Maxim. f. biserrata Shan & Yuan, 10g of Agkistrodon acutus (Guenther), 10g of Zaocys dhumnades (Cantor), 9g of Asarum sieboldii Miq., 18g of Poria cocos (Schw.) Wolf, 15g of Lonicera japonica Thunb., 9g of Gardenia jasminoides Ellis, 8g of Glycyrrhiza uralensis Fisch.

[0169] 81. Control Group 78: Chinese medicine for treating neurovascular headache with application number CN200810157590.3: 30 - 40g of Pueraria lobata, 18 - 20g of Ligusticum chuanxiong, 18 - 20g of Angelica dahurica, 15 - 18g of Chrysanthemum morifolium, 10 - 15g of Corydalis yanhusuo, 20 - 30g of Uncaria rhynchophylla, 15 - 20g of Achyranthes bidentata, 3 - 6g of Asarum sieboldii, 10 - 15g of Scutellaria baicalensis, 10 - 15g of Ligusticum sinense, 10 - 15g of Alisma orientale.

[0170] 82. Control Group 79: Oral preparation for treating headache and its preparation method with application number CN200810055070.1: 200 - 500 of Ligusticum chuanxiong, 300 - 500 of Radix Paeoniae Alba Praeparata, 200 - 500 of Angelica dahurica, 30 - 50 of Scorpio, 30 - 50 of Scolopendra subspinipes, 20 - 60 of Piper longum, 50 - 160 of Bombyx batryticatus, 60 - 120 of Radix Glycyrrhizae Praeparata, 120 - 180 of Vitex trifolia, 100 - 160 of Ligusticum sinense, 24 - 35 of Asarum sieboldii, 100 - 160 of Corydalis yanhusuo, 80 - 120 of Bupleurum chinense, 60 - 80 of Mentha haplocalyx, 20 - 53 of medicinal adjuvant.

[0171] 83. Control Group 80: Chinese medicine preparation for treating headache and its preparation method with application number CN200810104548.5: 15g of Ligusticum chuanxiong, 9g of Gastrodia elata, 15g of Corydalis yanhusuo, 12g of Angelica dahurica, 10g of Radix Paeoniae Rubra, 20g of Rehmannia glutinosa, 15g of Angelica sinensis, 9g of Flos Carthami, 15g of Salvia miltiorrhiza, 9g of Saposhnikovia divaricata, 9g of Asarum sieboldii, 15g of Pueraria lobata, 12g of Lonicera japonica.

[0172] 84. Control Group 81: Chinese medicine for treating intractable headache with application number CN200710132335.9: 5g of Scorpio, 12g each of Angelica dahurica, Notopterygium incisum, Ligusticum chuanxiong, 24g of Radix Paeoniae Alba, 30g of Concha Margaritifera Usta, 15g of Corydalis yanhusuo, 12g of Pheretima aspergillum, 5g of Radix Glycyrrhizae.

[0173] 85. Control Group 82: Chinese medicine for treating muscle contraction headache with application number CN200710132327.4: 10g each of Bupleurum chinense and Cyperus rotundus, 30g each of Radix Paeoniae Alba and Pueraria lobata, 6g of Radix Glycyrrhizae, 25g of Ligusticum chuanxiong, 15g each of Glossy Privet Fruit, Caulis Polygoni Multiflori, and Salvia miltiorrhiza, 10g each of Tribulus terrestris, Vitex trifolia, and Chrysanthemum morifolium.

[0174] 86. Control Group 83: Chinese medicine for treating liver - depression headache with application number CN200710132334.4: 15g of Bupleurum chinense, 10g each of Codonopsis pilosula, Scutellaria baicalensis, Pinellia ternata, Ligusticum chuanxiong, Corydalis yanhusuo, Uncaria rhynchophylla, and Cyperus rotundus, 12g of Radix Paeoniae Alba, 6g of Radix Glycyrrhizae.

[0175] 87. Control group 84: Application No. CN200610121719.6 A new method for treating headache with bupleurum, chuanxiong, angelica dahurica, angelica sinensis and chrysanthemum: bupleurum 15g, chuanxiong 15g, angelica dahurica 10g, angelica sinensis 15g, cyperus rotundus 9g, chrysanthemum 15g, salvia miltiorrhiza 15g, magnetite 20g, red peony root 10g, white peony root 12g, Millettia reticulata 15g, Leonurus japonicus 15g, Uncaria rhynchophylla 12g, and liquorice 8g.

[0176] 88. Control group 85: Application No. CN200610121717.7 A new method for treating headache with Gastrodia elata, cicada skin, licorice and processed white atractylodes for relieving menstruation and pain: Chuanxiong 15g, Gastrodia elata 9g, cicada skin 9g, licorice 8g, processed white atractylodes 9g, Uncaria rhynchophylla 12g, Bombyx batryticatus 9g, earthworm 9g, white peony root 12g, and Pueraria root 15g.

[0177] 89. Control group 86: Application No. CN200610077587.1 A medicament for treating tension headache and its preparation method: 2.5-4.5 parts of Cyperus rotundus, 3-4.5 parts of Paeonia lactiflora, 2.5-4.5 parts of Ligusticum chuanxiong, 3-4.5 parts of Angelica sinensis, 2.5-4 parts of Corydalis yanhusuo, 0.5-1.5 parts of Asarum, 1.5-3 parts of Angelica dahurica, 1.5-3 parts of Scutellaria baicalensis, and 1-3 parts of Bupleurum.

[0178] 90. Control group 87: Application No. CN200410047135.X Oral Chinese medicine preparation for treating vascular headache and migraine and its preparation method: Chuanxiong 15g, Bupleurum 15g, Angelica dahurica 10g, Cyperus rotundus 9g, White Peony 12g, Prunus mume 9g, White mustard seed 10g, Licorice 8g.

[0179] 91. Control group 88: Application No. CN03147797.6 A traditional Chinese medicine for treating cervical spondylosis and its preparation method: 9g of Gastrodia elata, 12g of Uncaria rhynchophylla, 15g of Ligusticum chuanxiong, 15g of Corydalis yanhusuo, 15g of Pueraria root and 10g of Angelica dahurica.

[0180] 92. Control group 89: Authorization number CN1100548C, a Chinese medicine composition for treating intractable headache: stir-fried peach kernel 10-15, safflower 10-15, angelica 15-20, chuanxiong 10-15, fructus aurantii 15-20, angelica dahurica 15-20, raw rehmannia 20-25, bupleurum 15-20, red peony root 15-20, platycodon 15-20, kudzu root 15-20, asarum 3-5, vitex rotundus 10-15, liquorice 6-10, white peony root 15-20, rehmannia glutinosa 15-20, evodia rutaecarpa 10-15, atractylodes 10-15, salvia miltiorrhiza 30-40, astragalus 20-25, atractylodes 15-20, and saposhnikovia divaricata 10-15.

[0181] Control Group 90: A traditional Chinese medicine for treating headache and trigeminal neuralgia, authorized by Patent No. CN1052890C: Earthworm 4.76g, Typhonium Rhizome 6.76g, Chuanxiong Rhizome 11.26g, Jackinthepulpit Tuber 6.76g, Red Peony Root 6.76g, Asarum Herb 2.82g, Corydalis Rhizome 6.76g, Heracleum Root 6.76g, Safflower 5.4g, Falsenettleleaf Schizonepeta Herb 6.76g, Peach Kernel 5.4g, Dahurian Angelica Root 6.76g, Chinese Angelica Root 6.76g, Scorpio 4.76g, Gastrodia Tuber 6.76g, Bombyx Batryticatus 4.76g.

[0182] Control Group 91: A traditional Chinese medicine compound of Xiongzhi for treating migraine, its capsules, pills and powders, and preparation methods, with application number CN201910645256.0: Raw White Peony Root 15 - 25 parts, Prepared Licorice Root 5 - 15 parts, Chrysanthemum 5 - 15 parts, Chuanxiong Rhizome 15 - 25 parts, Mentha Haplocalyx Briq. 5 - 15 parts, Dahurian Angelica Root 10 - 20 parts, Notopterygium Root 5 - 15 parts, Asarum Herb 4 - 6 parts, Bupleurum Root 10 - 20 parts, Cyperus Rotundus L. 5 - 15 parts, Curcuma Root 5 - 15 parts, Moutan Bark 5 - 15 parts, Scutellaria Baicalensis Georgi 5 - 15 parts, Ligusticum Wallichii Franch. 5 - 15 parts, Falsenettleleaf Schizonepeta Herb 5 - 15 parts, Corydalis Rhizome 10 - 20 parts, and Pyrola Herb 10 - 20 parts.

[0183] Control Group 92: A traditional Chinese medicine preparation for treating hypertension, authorized by Patent No. CN104324223B: Forsythia Fruit 9g, Tangerine Peel 9g, Safflower 9g, Earthworm 9g, Eclipta Prostrata L. 15g, Red Peony Root 10g, Ophiopogon Root 18g, Scrophularia Root 12g, Pinellia Tuber 9g, Pueraria Root 15g, Salvia Miltiorrhiza Bunge 15g, Chuanxiong Rhizome 15g, Leonurus Artemisia (Lour.) S. Y. Hu 15g, Astragalus Membranaceus (Fisch.) Bunge 20g, Corydalis Rhizome 15g, Plantago Seed 10g, Ramulus Mori 10g, Cortex Lycii Radicis 12g, Taxillus Chinensis (DC.) Danser 12g, Codonopsis Pilosula (Franch.) Nannf. 15g, Dahurian Angelica Root 10g, Phellodendron Bark 12g, Cassia Seed 15g, Plastrum Testudinis 20g, and Licorice Root 8g.

[0184] Control Group 93: A traditional Chinese medicine capsule for clearing the brain and relieving pain and its preparation method, with application number CN201510393279.9: Chuanxiong Rhizome 25%, Gastrodia Tuber 10% - 12%, Asarum Herb 8% - 10%, Dahurian Angelica Root 10% - 12%, Panax Notoginseng (Burkill) F. H. Chen ex C. Chow 10% - 12%, Pueraria Root 13% - 15%, White Peony Root 18% - 20%.

[0185] Control Group 94: A drug for treating cervical spondylosis and its preparation method, authorized by Patent No. CN102743703B: Ramulus Cinnamomi 80 - 120 parts, White Peony Root 100 - 180 parts, Pueraria Root 200 - 340 parts, Chuanxiong Rhizome 100 - 160 parts, Rhizoma Zedoariae 80 - 120 parts, Corydalis Rhizome 100 - 160 parts, Semen Ziziphi Spinosae 170 - 230 parts, Astragalus Membranaceus (Fisch.) Bunge 170 - 220 parts, Chinese Angelica Root 90 - 150 parts, Poria Cocos (Schw.) Wolf 170 - 210 parts, Bupleurum Root 90 - 110 parts, Scutellaria Baicalensis Georgi 80 - 130 parts.

[0186] Control Group 95: Application No. CN201210114326.8, A Traditional Chinese Medicine for Treating Migraine and Its Preparation Method: 20g of Smilax glabra, 15g of Glehnia littoralis, 9g of Gastrodia elata, 12g of Paeonia lactiflora, 15g of Spatholobus suberectus, 15g of Achyranthes bidentata, 15g of Moutan cortex, 9g of Scorpio, 8g of Ginger, 15g of Ligusticum wallichii, 9g of Schisandra chinensis, 15g of Cassia obtusifolia, 15g of Trichosanthes kirilowii, 15g of Angelica sinensis, 9g of Saposhnikovia divaricata, 12g of Phellodendron amurense, 12g of Atractylodes macrocephala, 10g of Angelica dahurica, 9g of Glossy privet fruit, 20g of Mulberry leaf, 9g of Schizonepeta tenuifolia, 15g of Pueraria lobata, 9g of Exocarpium citri grandis, 12g of Anemarrhena asphodeloides, and 9g of Heracleum hemsleyanum.

[0187] Control Group 96: Authorization No. CN100571729C, A Traditional Chinese Medicine for Treating Migraine: 15g of Cornu saigae tataricae, 15g of Ligusticum wallichii, 9g of Scorpio, 9g of Scolopendra, 12g of Paeonia lactiflora, 9g of Bombyx batryticatus, 10g of Angelica dahurica, 12g of Uncaria rhynchophylla, 6g of Panax notoginseng, 20g of Rehmannia glutinosa, and 10g of Honey-fried licorice root.

[0188] Control Group 97: Application No. CN200910127487.9, A Traditional Chinese Medicine Preparation for Treating Migraine: 15g of Ligusticum wallichii, 10g of Angelica dahurica, 9g of Saposhnikovia divaricata, 9g of Notopterygium incisum, 12g of Vitex trifolia var. simplicifolia, 15g of Pueraria lobata, Scorpio, Bombyx batryticatus, and 9g of Gastrodia elata each.

[0189] Control Group 98: Observation on the Efficacy of Treating 68 Cases of Chronic Tension-Type Headache with Headache Prescription. Modification of the Headache Prescription: 10g each of Ligusticum wallichii, Angelica dahurica, Notopterygium incisum, Ligusticum sinense, Bupleurum chinense, Corydalis yanhusuo, and Cyperus rotundus, 15g each of Paeonia lactiflora and Pueraria lobata, and 5g each of Asarum sieboldii and Licorice root.

[0190] Control Group 99: Traditional Chinese Medicine Treatment of Chronic Headache: 15 of Ligusticum wallichii, 10 of Angelica dahurica, 9 of Beauveria bassiana, 9g of Gastrodia elata, 15g of Pueraria lobata, 9g of Asarum sieboldii, 15g of Corydalis yanhusuo, 15g of Bupleurum chinense, and 9g of Scorpio.

[0191] Control Group 100: Efficacy Observation on Treating 43 Cases of Tension-Type Headache with Chuanxiong Shuqiao Granules. Chuanxiong Shuqiao Granules: 15g of Ligusticum wallichii, 15g of Corydalis yanhusuo, 15g of Pueraria lobata, 9g of Ligusticum sinense, 15g of Achyranthes bidentata, 12g of Paeonia lactiflora, 10g of Angelica dahurica, 15g of Salvia miltiorrhiza, 15g of Bupleurum chinense, 9g of Gentiana scabra, 15g of Chrysanthemum morifolium, 9g of Gastrodia elata, and 12g of Uncaria rhynchophylla.

[0192] Control Group 101: Randomized Parallel Control Study on Treating Blood Stasis Headache with Huoxue Zhitong Decoction. Huoxue Zhitong Decoction: 12g each of Prunus persica, Angelica sinensis, Angelica dahurica, Olibanum, Paeonia lactiflora, and Myrrha, 15g each of Rehmannia glutinosa, Fructus aurantii, Corydalis yanhusuo, Paeonia lactiflora, and Cyperus rotundus, 10g of Ligusticum wallichii, and 6g each of Licorice root and Carthamus tinctorius.

[0193] Control Group 102: Talks on the Treatment Experiences of Renowned Traditional Chinese Medicine Doctors Nationwide - Lu Yongbing's Treatment of Headache - Geju Xiongma Decoction. Geju Xiongma Decoction: 30 g of Pueraria lobata, 15 g each of Chrysanthemum morifolium, Paeonia lactiflora, Bombyx batryticatus, Rehmannia glutinosa, and Salvia miltiorrhiza, 10 g each of Ligusticum chuanxiong, Scutellaria baicalensis, Gastrodia elata, and Angelica dahurica, 5 g of Glycyrrhiza uralensis.

[0194] Control Group 103: Treating Tension Headache with the Method of Soothing the Liver, Alleviating Spasm, and Dispersing Wind to Stop Pain. Ingredients: 15 g each of Ligusticum chuanxiong, Notopterygium incisum, Angelica dahurica, Schizonepeta tenuifolia, Pueraria lobata, Bupleurum chinense, Cyperus rotundus, Ziziphus jujuba var. spinosa, Glycyrrhiza uralensis, and Angelica sinensis, 5 g of Asarum sieboldii, 20 g of Paeonia lactiflora.

[0195] Control Group 104: Clinical Observation on Treating Migraine with Self - formulated Headache Prescription Combined with Sibelium. Self - formulated Headache Prescription: 15 g of Ligusticum chuanxiong, 15 g of Poria cocos, 15 g of Angelica dahurica, 15 g of Gastrodia elata, 15 g of Angelica sinensis, 15 g of Salvia miltiorrhiza, 10 g of Ligusticum sinense, 10 g of Pueraria lobata, 10 g of Bupleurum chinense, 10 g of Paeonia lactiflora, 10 g of Cyperus rotundus, 10 g of Vitex trifolia var. simplicifolia, 6 g of Evodia rutaecarpa, 6 g of Glycyrrhiza uralensis.

[0196] Control Group 105: Treating 40 Cases of Primary Headache with Integrated Traditional Chinese and Western Medicine. Ingredients: 15 g of Gastrodia elata, 12 g of Chrysanthemum morifolium, 15 g of Rehmannia glutinosa, 15 g of Paeonia lactiflora, 9 g of Bupleurum chinense, 12 g of Scutellaria baicalensis, 6 g of Cyperus rotundus, 15 g of Angelica dahurica, 8 g of Gardenia jasminoides, 30 g of Margarita, 15 g of Alisma orientale, 30 g of Polygonatum sibiricum, 15 g of Ligusticum chuanxiong, 3 g of Asarum sieboldii, 12 g of Corydalis yanhusuo.

[0197] Control Group 106: Efficacy of Xiongzhi Decoction in Treating Migraine and Its Influence on Electroencephalogram and Brainstem Evoked Potential. Xiongzhi Decoction: 20 g of Ligusticum chuanxiong, 10 g of Angelica dahurica, 10 g of Paeonia veitchii, 10 g of Paeonia lactiflora, 10 g of Corydalis yanhusuo, 10 g of Prunella vulgaris, 10 g of Vitex trifolia var. simplicifolia, 10 g of Ligusticum sinense, 10 g of Mentha haplocalyx, 10 g of Chrysanthemum morifolium, 10 g of Bupleurum chinense, 3 g of Asarum sieboldii, 5 g of Saposhnikovia divaricata, 10 g of Poria cocos, 15 g of Pueraria lobata.

[0198] Control Group 107: Randomized Parallel Controlled Study on Treating Migraine with Qingnao Headache Decoction. Qingnao Headache Decoction: 10 g each of Ligusticum chuanxiong, Angelica dahurica, Paeonia veitchii, Paeonia lactiflora, Corydalis yanhusuo, Prunella vulgaris, Vitex trifolia var. simplicifolia, and Ligusticum sinense, 5 g of Mentha haplocalyx, 10 g each of Chrysanthemum morifolium and Bupleurum chinense, 3 g of Asarum sieboldii, 5 g of Saposhnikovia divaricata, 10 g of Poria cocos, 15 g of Pueraria lobata.

[0199] Control Group 108: Treating 360 Cases of Vascular Headache with Tongluo Jiejing Decoction. Tongluo Jiejing Decoction: 30 g each of Paeonia lactiflora and Uncaria rhynchophylla (added later), 20 g each of Ligusticum chuanxiong, Salvia miltiorrhiza, and Pueraria lobata, 15 g each of Saposhnikovia divaricata and Corydalis yanhusuo, 10 g each of Angelica dahurica, Glycyrrhiza uralensis, Pheretima aspergillum, Scorpio, Curcuma aromatica, and Citrus medica var. sarcodactylis, 6 g of Asarum sieboldii.

[0200] Control Group 109: Treating 108 Cases of Vascular Headache with Shugan Jiejing Decoction. Shugan Jiejing Decoction: 12 g each of Bupleurum chinense, Cyperus rotundus, Angelica dahurica, and Ligusticum chuanxiong, 15 g each of Corydalis yanhusuo and Paeonia lactiflora, 3 g of Glycyrrhiza uralensis.

[0201] Group 110: 67 cases of migraine treated with Qianxun Wuxie Capsules taken with Tou Tong Su Xiao Tang. Tou Tong Su Xiao Tang: 15 - 30 g each of Ligusticum wallichii, Pueraria lobata, Achyranthes bidentata, and Astragalus membranaceus; 5 - 10 g of Carthamus tinctorius; 10 - 20 g of Gastrodia elata; 6 - 10 g each of Asarum sieboldii, Angelica dahurica, and Saposhnikovia divaricata; 6 - 15 g each of Corydalis yanhusuo, Curcuma aromatica, Angelica sinensis, and Paeonia lactiflora; 6 g of Glycyrrhiza uralensis.

[0202] Group 111: 168 cases of vascular headache treated with Tongqiao Annao Tang. Tongqiao Annao Tang: 30 g of Salvia miltiorrhiza, 30 g of Ligusticum wallichii, 15 g each of Radix Paeoniae Rubra and Radix Paeoniae Alba, 20 g of Cyperus rotundus, 10 g of Bupleurum chinense, 10 g of Angelica dahurica, 10 g of Bombyx batryticatus, 12 g of Notopterygium incisum, 10 g of Chrysanthemum morifolium, 0.2 g of Borneol (separately wrapped and melted).

[0203] Group 112: 34 cases of menstrual headache treated with Liqi Huoxue Tang. Liqi Huoxue Tang: 15 g each of Bupleurum chinense, Pheretima aspergillum, Corydalis yanhusuo, Cyperus rotundus, Angelica sinensis, and Angelica dahurica; 10 g each of Prunus persica, Scorpio, and Gastrodia elata; 20 g each of Radix Paeoniae Alba, Ligusticum wallichii, Salvia miltiorrhiza, Os Draconis, and Concha Ostreae.

[0204] Group 113: Clinical observation on 33 cases of vascular headache treated with "Liqi Tongqiao Tang". Liqi Tongqiao Tang: 30 g of Salvia miltiorrhiza, 20 g of Ligusticum wallichii, 20 g of Radix Paeoniae Alba, 15 g of Cyperus rotundus, 15 g of Corydalis yanhusuo, 25 g of Poria cocos, 25 g of Saposhnikovia divaricata, 10 g of Semen Sinapis Albae, 15 g of Notopterygium incisum, 15 g of Bupleurum chinense, 10 g of Angelica dahurica, 5 g of Sanqi powder.

[0205] Group 114: One case of headache treated with "Qufeng Zhitong Fang". Qufeng Zhitong Fang: 15 g of Ligusticum wallichii, 20 g of Pueraria lobata, 20 g of Angelica dahurica, 20 g of Radix Paeoniae Alba, 10 g of Pinellia ternata, 5 g of Scutellaria baicalensis, 6 g of Glycyrrhiza uralensis, 5 g of Scorpio, 1 Scolopendra, 10 g of Bombyx batryticatus.

[0206] Group 115: Clinical efficacy observation on tension headache treated with the self - made prescription of Professor Tao Genyu: 12 g of Bupleurum chinense, 20 g of Ligusticum wallichii, 15 g of Angelica sinensis, 15 g of Radix Paeoniae Alba, 12 g of Atractylodes macrocephala, 20 g of Gypsum fibrosum, 30 g of Albizia julibrissin, 6 g of Asarum sieboldii, 20 g of Pueraria lobata, 12 g of Angelica dahurica, 6 g of Bombyx batryticatus, 6 g of Scorpio.

[0207] Group 116: 60 cases of cerebrovascular spasm headache treated with Geju Xiongma Tang. Geju Xiongma Tang: 40 g of Pueraria lobata, 15 g of Chrysanthemum morifolium, 6 g of Ligusticum wallichii, 10 g of Gastrodia elata, 20 g of Radix Paeoniae Alba, 5 g of Glycyrrhiza uralensis, 15 g of Bombyx batryticatus, 8 g of Angelica dahurica, 10 g of Scutellaria baicalensis, 20 g of Rehmannia glutinosa, 20 g of Salvia miltiorrhiza.

[0208] Group 117: 56 cases of migraine treated with Chaihu Chuanxiong Yin. Chaihu Chuanxiong Yin: 15 g each of Bupleurum chinense, Angelica sinensis, Angelica dahurica, Bombyx batryticatus, Pueraria lobata, and Radix Paeoniae Alba; 30 g of Ligusticum wallichii; 7.5 g of Asarum sieboldii; 10 g each of Evodia rutaecarpa and Glycyrrhiza uralensis.

[0209] 121. Control Group 118: Optimization and Evaluation of the Process of the Standard Decoction and Granules of the Classical Famous Prescription Sanpian Decoction: Ligusticum wallichii 37.3 g, Paeonia lactiflora 18.65 g, Angelica dahurica 1.865 g, Prunus japonica 3.73 g, Cyperus rotundus 7.46 g, Bupleurum chinense 3.73 g, Semen sinapis alba 11.19 g, Glycyrrhiza uralensis 3.73 g.

[0210] 122. Control Group 119: Clinical Observation on the Treatment of Chronic Headache with the Self-made Yuanhu Dielong Decoction. Yuanhu Dielong Decoction: Corydalis yanhusuo, Pheretima aspergillum, Angelica dahurica, Bupleurum chinense, Acorus tatarinowii, Carthamus tinctorius, Prunus persica, Manis pentadactyla, each 10 g; Angelica sinensis, Rehmannia glutinosa, each 15 g; Ligusticum wallichii, Paeonia lactiflora, each 30 g; Scolopendra subspinipes mutilans 1 piece (ground and taken orally), Buthus martensii 2 g (ground and taken orally).

[0211] 123. Control Group 120: Efficacy Observation on the Treatment of 321 Cases of Migraine with Phlegm-stasis Type with Tongmai Zhitong Prescription. Tongmai Zhitong Prescription: Astragalus membranaceus 20 g, Angelica sinensis 12 g, Paeonia lactiflora 30 g, Ligusticum wallichii 30 g, Hirudo 6 g, Angelica dahurica 9 g, Pheretima aspergillum 6 g, Cinnamomum cassia 6 g, Bupleurum chinense 6 g, Pueraria lobata 15 g, Gastrodia elata 9 g, Cynanchum paniculatum 30 g, Rheum palmatum 30 g, Chrysanthema morifolium 9 g, Vitex trifolia var. simplicifolia 9 g.

[0212] 124. Control Group 121: Experience of Qin Yuehao in Treating Female Migraine from the Perspective of Wind-phlegm-stasis. Ligusticum wallichii 10 g, stir-fried Paeonia lactiflora 20 g, Semen sinapis alba 10 g, Angelica dahurica 10 g, Smilax glabra 20 g, Salvia miltiorrhiza 15 g, Bombyx batryticatus 10 g, Xanthium sibiricum 10 g.

[0213] 125. Control Group 122: Clinical Observation on the Treatment of 60 Cases of Migraine with the Self-made Headache Prescription. Bupleurum chinense 15 g, Ligusticum wallichii 10 g, Angelica dahurica 10 g, Saposhnikovia divaricata 10 g, Asarum sieboldii 4 g, Pueraria lobata 30 g, Ramulus mori 30 g, Paeonia lactiflora 25 g, Buthus martensii 5 g, Scolopendra subspinipes mutilans 2 pieces, Salvia miltiorrhiza 20 g, Angelica sinensis 10 g, Astragalus membranaceus 30 g, roasted Glycyrrhiza uralensis 10 g.

[0214] 126. Control Group 123: Treatment of Migraine with Traditional Chinese Medicine Headache Powder. Ligusticum wallichii 20 g, Angelica dahurica 20 g, Asarum sieboldii 3 g, Notopterygium incisum 20 g, Saposhnikovia divaricata 20 g, Uncaria rhynchophylla 15 g, roasted Buthus martensii 15 g, Salvia miltiorrhiza 20 g, Pheretima aspergillum 20 g, Bombyx batryticatus 20 g, Evodia rutaecarpa 20 g, Gypsum fibrosum 30 g, Paeonia lactiflora 15 g, Cyperus rotundus 20 g.

[0215] 127. Control Group 124: Experience in Treating Migraine with Sanpian Decoction by Learning from the Elder Mr. Zhang Zilin. Paeonia lactiflora 15 g, Ligusticum wallichii 30 g, Prunus japonica 3 g, Bupleurum chinense 3 g, Semen sinapis alba 9 g, Cyperus rotundus 6 g, Glycyrrhiza uralensis 3 g, Angelica dahurica 1.5 g.

[0216] 128. Control Group 125: Treatment of 38 Cases of Migraine with Xiongzhi Shaoyao Decoction. Xiongzhi Shaoyao Decoction: Ligusticum wallichii 15, Angelica dahurica 15, raw Paeonia lactiflora 15 g, Bombyx batryticatus 10 g, Glycyrrhiza uralensis 7.5 g.

[0217] Control Group 126: Treatment of 41 cases of vascular headache by syndrome differentiation: 15 grams of Ligusticum chuanxiong, 15 grams of Angelica dahurica, 15 grams of Angelica sinensis, 10 grams of Bombyx batryticatus, 10 grams of Chrysanthemum morifolium, 15 grams of Paeonia lactiflora, 10 grams of Ligusticum sinense, 10 grams of Vitex trifolia, 6 grams of Glycyrrhiza uralensis.

[0218] Control Group 127: Efficacy observation of treating 84 cases of migraine with self-made Chuanxiong Gastrodia Decoction. Chuanxiong Gastrodia Decoction: 30 - 40 grams of Ligusticum chuanxiong, 15 grams of Gastrodia elata, 12 grams of Notopterygium incisum, 15 grams of Angelica dahurica, 9 grams of Asarum sieboldii, 12 grams of Pheretima aspergillum, 15 grams of Corydalis yanhusuo vinegar-processed, 15 grams of Paeonia lactiflora, 10 grams of Glycyrrhiza uralensis.

[0219] Control Group 128: Seven prescriptions of traditional Chinese medicine for pain relief. Prescription for pain relief: 15 grams of Gastrodia elata, 15 grams of Ligusticum chuanxiong, 15 grams of Angelica dahurica, 15 grams of Aconitum carmichaelii, 15 grams of Corydalis yanhusuo.

[0220] Control Group 129: Treatment of 53 cases of angioneurotic headache with self-made Chuanxiong Guiwei Tianma Baizhi Decoction. Chuanxiong Guiwei Tianma Baizhi Decoction: It consists of 20 grams of Ligusticum chuanxiong, 15 grams each of Angelica sinensis, Paeonia lactiflora, and Salvia miltiorrhiza, 10 grams each of Gastrodia elata, Bombyx batryticatus, Saposhnikovia divaricata, Angelica dahurica, and Pinellia ternata, 8 grams of Bupleurum chinense, 4 grams each of Asarum sieboldii and Glycyrrhiza uralensis.

[0221] Control Group 130: Treatment experience of intractable internal injury headache: 200 grams of Astragalus membranaceus roasted, 30 grams each of Ligusticum chuanxiong and Rehmannia glutinosa, 6 grams each of Carthamus tinctorius and Angelica dahurica, 15 grams of Angelica sinensis, 12 grams of Corydalis yanhusuo, 10 grams of Cyperus rotundus processed, 3 grams of Asarum sieboldii.

[0222] Control Group 131: Clinical study of treating migraine with blood stasis blocking collaterals syndrome by Chuanxiong Huoxue Tongqiao Capsule. Chuanxiong Huoxue Tongqiao Capsule: 15 grams of Ligusticum chuanxiong, 9 grams of Carthamus tinctorius, 15 grams of Salvia miltiorrhiza, 10 grams of Angelica dahurica, 9 grams of Tribulus terrestris, 15 grams of Pueraria lobata, 15 grams of Corydalis yanhusuo.

[0223] Control Group 132: Treatment of 80 cases of migraine by modified Sanpian Decoction: 30 grams of large Ligusticum chuanxiong, 1.5 grams of Angelica dahurica, 3 grams of Bupleurum chinense, 6 grams of Cyperus rotundus roasted, 3 grams of Glycyrrhiza uralensis, 15 grams of Paeonia lactiflora, 3 grams of Prunus japonica, 10 grams of Semen sinapis alba.

[0224] Control Group 133: Treatment of angioneurotic headache with Qufeng Huoxue Decoction. Qufeng Huoxue Decoction: 30 grams each of Ligusticum chuanxiong and Paeonia lactiflora, 15 grams each of Angelica sinensis, Angelica dahurica, and Paeonia lactiflora rubra, 12 grams of Heracleum hemsleyanum, 6 grams of Asarum sieboldii, 8 grams of Mentha haplocalyx, 10 grams of Bombyx batryticatus, 3 grams of Glycyrrhiza uralensis.

[0225] Control Group 134: Treatment of 18 cases of cluster headache with Qufeng Dingtong Decoction. Qufeng Dingtong Decoction: 30 grams each of Ligusticum chuanxiong, Paeonia lactiflora, Gypsum fibrosum, and Salvia miltiorrhiza, 15 grams each of Angelica dahurica, Scutellaria baicalensis, Pheretima aspergillum, Bombyx batryticatus, and Vitex trifolia, 5 grams of Typhonium giganteum, 6 grams each of Carthamus tinctorius and Glycyrrhiza uralensis.

[0226] 138. Control group 135: 175 cases of headache were treated with modified Sanpian Decoction: 50g of Chuanxiong, 25g of white peony root, 2.5g of Angelica dahurica, 15g of white mustard seeds, 5g of Bupleurum, 5g of Prunus mume, 10g of Cyperus rotundus, and 5g of Licorice.

[0227] 139. Control group 136: Lv Tongjie's experience in treating headache with Huoxue Shufeng Decoction: Angelica 12g, Chuanxiong 15g, White Peony 12g, Raw Rehmannia 20g, Cyperus 9g, Prunella Vulgaris 9g, Raw Dragon Bone 20g, Prunella Vulgaris 15g, Pinellia 9g, Angelica dahurica 10g, White Swallow 9g, Mustard Seed 15g.

[0228] 140. Control group 137: Xifeng Zhentong Decoction for the treatment of trigeminal neuralgia, Xifeng Zhentong Decoction: 6 grams of Gastrodia elata, 6 grams of Uncaria rhynchophylla (added later), 3 grams of whole scorpion (pounded into powder and swallowed), 1 centipede (pounded into powder and swallowed), 12 grams of raw white peony root, 3 grams of asarum, 6 grams of white aconite, 6 grams of bombyx batryticatus, 6 grams of white tribulus, 10 grams of chrysanthemum, 6 grams of Chuanxiong, 6 grams of Bupleurum, 6 grams of Saposhnikovia divaricata, 6 grams of Angelica dahurica, and 6 grams of Notopterygium wilfordii.

[0229] 141. Control group 138: Chief physician Cai Youjing used Xiongshao Analgesic Decoction to treat vascular headache. Xiongshao Analgesic Decoction: 30g of Chuanxiong, 15g of White Peony Root, 10g of Angelica Dahurica, 10g of Notopterygium Wilfordii, 10g of Bupleurum, 10g of Cyperus rotundus, 15g of Uncaria rhynchophylla, 30g of Mother of Pearl, and 3g of Raw Licorice.

[0230] 142. Control group 139: Clinical application of the proven ancestral recipe for treating injuries: 12g of Angelica sinensis stir-fried with wine, 15g of Salvia miltiorrhiza, 10g of Eupolyphaga sinensis, 10g of red and white peony roots, 10g of processed Cyperus rotundus, 10g of Rhizoma Cyperi, 10g of Ligusticum chuanxiong stir-fried with wine, 10g of stir-fried Corydalis, 10g of Curcuma atractylodes, 3g of Panax notoginseng powder (swallow), 2.5g of scorpion (grind into powder and swallow).

[0231] 143. Control group 140: 31 cases of migraine were treated with Sanpian Decoction. Sanpian Decoction: 30g of Chuanxiong, 10g each of Angelica dahurica, White Peony Root, White Mustard Seed, Bupleurum Root, Bombyx Batryticatus, 6g each of Cyperus rotundus, Scorpion, Licorice, 2 centipedes (grinded into powder and taken with water), 3g each of Asarum and Prunus mume.

[0232] 144. Control group 141: vascular headache was treated with modified Siwu Decoction, modified Siwu Decoction: Angelica 12g, Ligusticum chuanxiong 15g, Rehmannia glutinosa 20g, White Peony 12g, Corydalis 15g, Asarum 9g, Semen Cassiae 15g, Bombyx Batryticatus 9g, Uncaria rhynchophylla 9g, Millettia reticulata 15g.

[0233] 145. Control group 142: Observation on the clinical efficacy of Toufengsan in treating headache. Toufengsan: 30-60g of Chuanxiong, 3-9g of Asarum, 30g of Angelica dahurica, 20g of Uncaria rhynchophylla (added later), 15g of Gastrodia elata, 30g of Corydalis yanhusuo, and 20g of White Peony Root.

[0234] 146. Control group 143: 116 cases of intractable headache were treated with Toufeng Zhentong Decoction. Toufeng Zhentong Decoction: 10g each of bupleurum, chrysanthemum, kudzu root, curcuma, cicada shell, and gastrodia, 18g of chuanxiong, 12g of angelica dahurica, 6-10g each of asarum and gastrodia, white peony root, and magnetite 20g.

[0235] 147. Control group 144: 40 cases of headache were treated with Xiongshaoxiezhi decoction. Xiongshaoxiezhi decoction: 10g of Chuanxiong, 10g of white peony root, 10g of Angelica dahurica, 10g of Pueraria root, 5g of whole scorpion, and 15g of Salvia miltiorrhiza.

[0236] 148. Control group 145: 28 cases of intractable headache were treated with Xiongzhi Pain-Relief Decoction. Xiongzhi Pain-Relief Decoction: 10-20g of Chuanxiong, 12g of Angelica dahurica, 15g of White Peony Root, 30g of Salvia miltiorrhiza, 5g of Asarum, 12g of Bombyx Batryticatus, 5g of Whole Scorpion, and 8g of Jiucao.

[0237] 149. Control group 146: Clinical observation on the treatment of migraine without aura with a self-made headache prescription. The self-made headache prescription: 10g of Notopterygium wilfordii, 3g of Asarum, 6g of Saposhnikovia divaricata, 12g of Angelica dahurica, 12g of Angelica sinensis, 6g of Parenchyma sphaerocephala, 20g of Astragalus membranaceus, 12g of Photinia serrulata, 10g of Bombyx batryticatus, 30g of Ligusticum chuanxiong, 30g of Pueraria root, and 6g of Licorice.

[0238] 150. Control group 147: A randomized parallel controlled study on the treatment of tension-type headache with headache mixture: 10g each of processed Gastrodia elata and whole scorpion, 1 centipede, 15g of Vitex rotundifolia, 10g of Angelica dahurica, 30g of Pueraria root, 10g of Corydalis yanhusuo, and 15g of Ligusticum chuanxiong.

[0239] 151. Control group 148: 60 cases of migraine were treated with Sanpian Zhitong Decoction. Sanpian Zhitong Decoction: 12g each of bupleurum, white peony root, saposhnikovia divaricata, chrysanthemum, notopterygium, pinellia, gardenia, 15g each of chuanxiong, vitex mangzi, yanhusuo, 10g each of earthworm and bombyx batryticatus, 20g of salvia miltiorrhiza, 8g of panax notoginseng powder (taken with water), and 4g of liquorice.

[0240] 152. Control group 149: 40 cases of muscle contraction headache were treated with Quntong Pills. Quntong Pills: Gastrodia elata 150g, Ligusticum chuanxiong 100g, Vitex mandibulariae 150g, Corydalis 100g, Angelica dahurica 100g, Prunella vulgaris 120g, Scorpio 150g, Tribulus terrestris 100g, Pueraria root 120g, Pheretima lumbar disc 120g, Arctium 100g, Safflower 100g, Platycodon grandiflorum 30g.

[0241] 153. Control group 150: 38 cases of migraine were treated with Piantong Decoction. Piantong Decoction: 15g of Chuanxiong, 10g each of Notopterygium wilfordii, Angelica dahurica, Corydalis yanhusuo, Gastrodia elata, Bombyx batryticatus, 5g of whole scorpion, 9g each of Bupleurum chinense and Cyathula capitata.

[0242] Group 151 control: Modified Chaihu Shugan Powder for treating migraine. Chaihu Shugan Powder: 9g of Bupleuri Radix, 9g of Chuanxiong Rhizoma, 8g of Cyperi Rhizoma, 9g of Aurantii Fructus Immaturus, 24g of Paeoniae Radix Alba, 15g of Glycyrrhizae Radix, 9g of Evodiae Fructus, 3g of Asari Radix et Rhizoma, 12g of Corydalis Rhizoma.

[0243] Group 152 control: 106 cases of migraine treated with Sanpian Tong Capsule. Sanpian Tong Capsule: 15g of Chuanxiong Rhizoma, 9g of Angelicae Dahuricae Radix, 9g of Ligustici Rhizoma, 9g of Asari Radix et Rhizoma, 12g of Viticis Fructus, 15g of Bupleuri Radix, 9g of Pheretima, 12g of Paeoniae Radix Alba, 9g of Cyperi Rhizoma, 9g of Sinapis Semen.

[0244] Group 153 control: Randomized parallel control study on treating migraine with the method of soothing the liver, relieving depression and promoting blood circulation. Prescription: 15g of Bupleuri Radix, 15g of Chuanxiong Rhizoma, 9g of Cyperi Rhizoma, 12g of Angelicae Sinensis Radix, 9g of Bombyx Batryticatus, 15g of Uncariae Ramulus cum Uncis, 12g of Paeoniae Radix Alba, 15g of Aurantii Fructus Immaturus, 9g of Curcumae Radix, 9g of Citri Reticulatae Pericarpium, 15g of Achyranthis Bidentatae Radix, 8g of Glycyrrhizae Radix.

[0245] Group 154 control: 50 cases of vascular headache treated with Chaiqiong Decoction. Chaiqiong Decoction: 15g of Bupleuri Radix, 10g of Chuanxiong Rhizoma, 10g of Gastrodiae Rhizoma, 10g of Angelicae Dahuricae Radix, 10g of Angelicae Sinensis Radix, 10g of Salviae Miltiorrhizae Radix, 10g of Pheretima, 10g of Cyperi Rhizoma, 10g of Viticis Fructus, 10g of Paeoniae Radix Alba, 10g of Glycyrrhizae Radix, 10g of Poria, 10g of Atractylodis Macrocephalae Rhizoma, 10g of Pinelliae Rhizoma.

[0246] Group 155 control: 36 cases of angioneurotic headache treated with the method of nourishing blood, promoting blood circulation, expelling wind and dredging collaterals. Prescription: 15g each of Chuanxiong Rhizoma, Angelicae Sinensis Radix, Paeoniae Radix Alba, Achyranthis Bidentatae Radix, Puerariae Lobatae Radix, 10g each of Angelicae Dahuricae Radix, Ligustici Rhizoma, Cicadae Periostracum, 6g of Asari Radix et Rhizoma, 3g of Scorpio (ground and taken orally).

[0247] Group 156 control: Modified Siwu Decoction for treating neural headache. Siwu Decoction: 18g of Angelicae Sinensis Radix, 12g each of Paeoniae Radix Alba, Angelicae Dahuricae Radix, Saposhnikoviae Radix, Chrysanthemi Flos, 15g each of Rehmanniae Radix Praeparata, Cyperi Rhizoma, 20g of Chuanxiong Rhizoma, 6g of Asari Radix et Rhizoma, 10g of Ligustici Rhizoma, 9g of Glycyrrhizae Radix.

[0248] Group 157 control: 56 cases of migraine treated with Tiaoxue Shufeng Decoction. Tiaoxue Shufeng Decoction: 30g of Paeoniae Radix Alba, 30g of Chuanxiong Rhizoma, 8g of Angelicae Dahuricae Radix, 6g of Bupleuri Radix, 10g of Notopterygii Rhizoma, 10g of Sinapis Semen, 6g of Pruni Semen, 6g of Cyperi Rhizoma, 3g of Glycyrrhizae Radix.

[0249] Group 158 control: Chronic headache treated with Toufukang Decoction. Toufukang Decoction: 30g each of Chuanxiong Rhizoma, Crataegi Fructus, Rehmanniae Radix Praeparata, Paeoniae Radix Alba, 25g of Corni Fructus, 12g each of Angelicae Sinensis Radix, Cyperi Rhizoma, Sinapis Semen, Angelicae Dahuricae Radix, Citri Reticulatae Pericarpium.

[0250] Group 159 control: 56 cases of headache treated with Modified Xiaoyao Powder. Modified Xiaoyao Powder: 15g of Bupleuri Radix, 12g of Paeoniae Radix Alba, 12g of Angelicae Sinensis Radix, 12g of Atractylodis Macrocephalae Rhizoma, 6g of Menthae Haplocalycis Herba, 15g of Perillae Folium, 9g of Cyperi Rhizoma, 10g of Angelicae Dahuricae Radix, 15g of Chuanxiong Rhizoma, 12g of Leonuri Herba, 8g of Glycyrrhizae Radix.

[0251] Control Group 160: 24 cases of menstrual headache were treated with the Tiaogan Huoxue Decoction. The Tiaogan Huoxue Decoction consists of: Bupleurum chinense 10 g, Paeonia lactiflora 12 g, Ligusticum chuanxiong 18 g, Angelica sinensis 10 g, Rehmannia glutinosa 15 g, Prunus persica 10 g, Gastrodia elata 10 g, Angelica dahurica 10 g, Salvia miltiorrhiza 12 g, Cyperus rotundus 10 g, Pheretima aspergillum 10 g, Scorpio 10 g, Os draconis 22 g, Concha ostreae 22 g.

[0252] Control Group 161: 56 cases of headache were treated with traditional Chinese medicine: Ligusticum chuanxiong 25 g, Notopterygium incisum 15 g, Angelica dahurica 15 g, Paeonia lactiflora 15 g, Bombyx batryticatus 15 g, Bupleurum chinense 10 g, Vitex trifolia 15 g, Ligusticum sinense 15 g, Glycyrrhiza uralensis 10 g.

[0253] Control Group 162: 39 cases of migraine were treated with the self - made Headache Decoction. The self - made Headache Decoction consists of: Angelica sinensis 12 g, Rehmannia glutinosa 12 g, Ligusticum chuanxiong 12 g, Paeonia lactiflora 12 g, Asarum sieboldii 6 g, Corydalis yanhusuo 15 g, Prunella vulgaris 30 g, Uncaria rhynchophylla 30 g, Cassia obtusifolia 30 g, Spatholobus suberectus 24 g, Angelica dahurica 12 g, Ligusticum sinense 12 g, Bupleurum chinense 12 g.

[0254] Control Group 163: 36 cases of migraine were treated with the Headache Decoction. The Headache Decoction consists of: Ligusticum chuanxiong 12 g, Angelica dahurica, Notopterygium incisum, Schizonepeta tenuifolia, Saposhnikovia divaricata each 10 g, Uncaria rhynchophylla 20 g, Paeonia lactiflora 15 g, Scorpio, Bombyx batryticatus, Mentha haplocalyx each 6 g, Asarum sieboldii 4 g, Glycyrrhiza uralensis 10 g.

[0255] Control Group 164: Efficacy observation on 116 cases of vascular headache treated with the self - made Zhenshou Decoction taken orally and externally smoked. The self - made Zhenshou Decoction consists of: Ligusticum chuanxiong 12 g, Paeonia lactiflora 20 g, Angelica dahurica 10 g, Arisaema cum bile 9 g, Panax notoginseng 6 g, Bombyx batryticatus 10 g, Chrysanthema morifolium 10 g, Tribulus terrestris 20 g.

[0256] Control Group 165: 65 cases of vascular headache were treated with integrated traditional Chinese and Western medicine: Ligusticum chuanxiong 20 g, Angelica dahurica 15 g, Vitex trifolia 15 g, Tribulus terrestris 15 g, Bombyx batryticatus 10 g, Paeonia lactiflora 10 g, Concha ostreae 20 g, Asarum sieboldii 6 g.

[0257] Control Group 166: 65 cases of migraine were treated with the modified Wubai Decoction. The Wubai Decoction consists of: Angelica dahurica 6 g, Sinapis alba 12 g, Paeonia lactiflora 12 g, Typhonium giganteum 6 g, Bombyx batryticatus 12 g, Ligusticum chuanxiong 15 g, Prunus japonica 9 g, Bupleurum chinense 9 g, Pheretima aspergillum 12 g, Ligusticum sinense 12 g, Glycyrrhiza uralensis 6 g, Asarum sieboldii 3 g.

[0258] Control Group 167: 98 cases of migraine were treated with traditional Chinese medicine: Salvia miltiorrhiza 15 g, Paeonia lactiflora 18 g, Ligusticum chuanxiong, Tribulus terrestris each 12 g, Pueraria lobata 30 g, Typhonium giganteum preparata, Bombyx batryticatus, Scorpio each 6 g, Pheretima aspergillum 10 g, Arisaema cum bile 8 g, Glycyrrhiza uralensis preparata 5 g.

[0259] 171. Control group 168: 76 cases of vascular headache were treated with Dingtong Decoction. Dingtong Decoction: 30g each of magnetite (pre-decocted), raw white peony root, salvia miltiorrhiza, and sunflower receptacle, 15g each of gastrodia elata, yanhusuo, chuanxiong, red peony root, earthworm, and bombyx batryticatus, 10g of processed arisaema, 6g each of scorpion, leech, and liquorice.

[0260] 172. Control group 169: Clinical observation on the treatment of vestibular migraine with the method of extinguishing wind and relieving spasm: Gastrodia elata 30g, Pueraria root 45g, White peony root 10g, Rhodiola rosea 20g, Pinellia ternata 9g, stir-fried Atractylodes macrocephala 20g, Ligusticum chuanxiong 12g, Corydalis yanhusuo 10g, Licorice root 10g.

[0261] 173. Control group 170: 62 cases of vascular neurosis headache were treated with a self-prescribed prescription: 20g of Chuanxiong, 3g of Asarum, 10g of Angelica dahurica, 15g of Chrysanthemum, 10g of Bombyx Batryticatus, 20g of Gypsum, 20g of White Peony Root, 15g of Salvia miltiorrhiza, 10g of Angelica sinensis, 10g of Saposhnikovia divaricata, and 10g of Bupleurum.

[0262] 174. Control group 171: 50 cases of migraine were treated with Xiongshen Wubai Decoction. Xiongshen Wubai Decoction: 20g of Chuanxiong, 20g of Salvia miltiorrhiza, 15g of White Peony Root, 5g of Angelica dahurica, 10g of Bombyx Batryticatus, 10g of White Chrysanthemum, and 6g of White Mustard Seed.

[0263] 175. Control group 172: 92 cases of migraine were treated with Wuluotai method of promoting blood circulation, dispelling wind and resolving phlegm: 15g of Salvia miltiorrhiza, 18g of White Peony Root, 12g of Ligusticum chuanxiong, 30g of Pueraria lobata, 6g each of processed White Aconitum, Bombyx Batryticatus, and Scorpio, 10g of Pheretima, 12g of Tribulus Terrestris, 8g of Arisaema Cum Bile, and 5g of roasted Licorice Root.

[0264] 176. Control group 173: 32 cases of phlegm-stasis type migraine were treated with Tongmai Zhitong prescription. Tongmai Zhitong prescription: Astragalus 20g, Angelica 12g, White Peony 30g, Ligusticum chuanxiong 30g, Hirudo 6g, Angelica dahurica 9g, Pheretima 6g, Cinnamon twig 6g, Bupleurum 6g, Pueraria lobata 15g, Gastrodia elata 9g, Cynanchum chinense 30g, Rhubarb 30g, Chrysanthemum 9g, Vitex rotundifolia 9g.

[0265] 177. Control group 174: Observation on the clinical efficacy of Ge Gen Quan Chong Tang in the treatment of migraine. Ge Gen Quan Chong Tang: Ge Gen Quan Chong Tang: 30-60g of Ge Gen, 30-60g of Bai Shao, 10g of Licorice, 10g of Chuanxiong, 15g of Angelica dahurica, 9g of Bupleurum, 30g of Semen Cassiae, 20g of Pheretima, 10g of Chrysanthemum, 20g of Achyranthes bidentata, 15g of Uncaria rhynchophylla (added last), 2-6g of Quan Chong Tang (ground into powder and taken with water).

[0266] 178. Control group 175: 46 cases of blood stasis type migraine were treated with modified Xionglong Decoction: 30g each of Chuanxiong, Pueraria root, Corydalis, and Achyranthes bidentata, 15g of earthworm, 3g of asarum, and 9g of Angelica dahurica.

[0267] 179. Control group 176: 34 cases of vascular headache were treated with Tianma Gegen Jiejing Decoction. Tianma Gegen Jiejing Decoction: Tianma 10g, Pueraria 20g, Chuanxiong 10g, White Peony 10g, Earthworm 10g, Corydalis 10g, Saposhnikovia 10g.

[0268] 180. Control group 177: 36 cases of intractable migraine were treated with Xiongshao Bange Decoction. Xiongshao Bange Decoction: 30g each of Chuanxiong, White Peony, Pinellia, Pueraria, Uncaria, and Semen Cassiae, 10g each of Asarum, Angelica Dahurica, and Licorice. Take 1 dose per day, decocted in water; 4g of whole scorpion and 2 centipedes.

[0269] 181. Control group 178: Clinical observation on the treatment of tension headache with Gegen decoction combined with behavioral intervention. Gegen decoction: Gegen 20g, Chuanxiong 15g, Angelica dahurica 9g, Curcuma 12g, Mentha 9g (added later), Cinnamon twig 6g, White peony root 12g, Scorpion 3g.

[0270] 182. Control group 179: Ge Gen Quan Chong Tang was used to treat vascular neurosis headache. Ge Gen Quan Chong Tang: Ge Gen Quan Chong Tang: 30-60g of Ge Gen, 30-60g of Bai Shao, 10g of Licorice, 10g of Chuanxiong, 15g of Angelica dahurica, 9g of Bupleurum, 30g of Semen Cassiae, 20g of Pheretima, 10g of Chrysanthemum, 20g of Achyranthes bidentata, 15g of Uncaria rhynchophylla (added last), 2-6g of Quan Chong Tang (ground into powder and taken with water).

[0271] 183. Control group 180: 50 cases of vascular neurosis headache were treated with modified analgesic decoction. Analgesic decoction: 15g each of Chuanxiong, Angelica dahurica, Ligusticum chuanxiong, Paeonia lactiflora, Paeonia lactiflora, Sinapis alba, Pueraria root, and 10g each of earthworm and scorpion.

[0272] 184. Control group 181: Headache Ning prescription: Chuanxiong 40g, Angelica dahurica 10g, Asarum 3g, Mentha 30g, Caulis Sinomenii 15g, Gastrodia elata 20g, Curcuma 12g, Fried Bombyx batryticatus 12g, Pheretima lumbarum 12g, Licorice root 6g.

[0273] 185. Control group 182: Flunarizine hydrochloride capsules (Xi'an Janssen Pharmaceutical Co., Ltd., national medicine standard H10930003, product batch number: PBJ1100) 1.25 mg / kg·d.

[0274] After grouping, the animals in each group were given drugs by gavage for 7 consecutive days, and the model group and the blank group were given equal volumes of distilled water. The body weight of the rats was measured, and the average body weight increase rate was calculated = [(average body weight after drug administration - average body weight before drug administration) / average body weight before drug administration] × 100%.

[0275] 2.3 Behavioral testing

[0276] After the last injection of nitroglycerin, behavior was observed and recorded.

[0277] 2.3.1 Determination of mechanical pain threshold

[0278] The mechanical pain threshold of the plantar surface of the hind paw of rats was measured using von Frey filaments. After the rats adapted to the environment (about 30 min), the von Frey filaments were vertically applied to the skin of the central area of the hind paw, starting from small units and gradually increasing the force value of the filaments. A positive reaction was defined as the rat withdrawing the paw, showing an avoidance reaction, scratching the filament with the paw, or licking the paw. The value corresponding to the positive reaction was recorded as a threshold, and each rat was repeatedly measured 3 times at intervals of at least 5 minutes. The average value of the 3 results was taken as the threshold for this time for subsequent statistical analysis.

[0279] 2.3.2 Measurement of thermal pain threshold

[0280] The thermal pain threshold of the plantar surface of rats was measured using a thermal radiation instrument, that is, the withdrawal latency of the hind paw was recorded. The experiment was officially started after the rats had no exploratory behavior after adapting to the device for about half an hour. The specific operation was as follows: the radiation intensity was set at 50%, the infrared radiation light spot was aligned with the central area of the hind paw of the rat, and the positive reaction was that the rat showed a paw withdrawal / licking paw reaction. At this time, the value was automatically recorded on the display screen of the device as the thermal pain paw withdrawal latency of the rat. To prevent the rats from being injured, the upper limit time of thermal radiation was set at 20 s. It was repeatedly measured 3 times at intervals of at least 5 minutes, and the average value of the 3 results was taken for subsequent statistical analysis.

[0281] 2.4 Detection of related indicators such as neurotransmitters in rat serum

[0282] On the 7th day after administration and 24 h after the behavioral test, all rats were anesthetized by injecting Zoletil and atropine intraperitoneally, and then blood was taken from the abdominal aorta. After standing for 30 min, the serum was separated by centrifugation at 3500 r / min for 15 min and stored at -80 °C for later use. According to the instructions of the enzyme-linked immunosorbent assay kit, the levels of 5-hydroxytryptamine (5-HT) and calcitonin gene-related peptide (CGRP) in the serum of rats in each group were detected.

[0283] 2.5 Results

[0284] 2.5.1 Effect of the present invention on the body weight of rats with chronic migraine: After administration in this example, most of them could improve the body weight loss of rats caused by long-term migraine. Among them, the improvement of the body weight loss of rats in the group of the present invention was more significant (P < 0.05). See Table 5.

[0285] Table 5: Effect of the present invention on the body weight of rats with chronic migraine

[0286]

[0287]

[0288]

[0289]

[0290]

[0291] Note: Compared with the model group, *P < 0.05, **P < 0.01

[0292] 2.5.2 Effect of the present invention on the mechanical pain threshold of rats with chronic migraine: The mechanical pain threshold was measured 30 min and 1 h after the last injection of nitroglycerin. Compared with the blank group, the mechanical pain threshold of the model group was significantly decreased (P < 0.05). Compared with the model group, the mechanical pain threshold of the present invention group was significantly increased, and the difference was statistically significant. It was found that the present invention group could increase the mechanical pain threshold of rats with chronic migraine. See Table 6.

[0293] Table 6: Effect of the present invention on the mechanical pain threshold of rats with chronic migraine

[0294]

[0295]

[0296]

[0297]

[0298]

[0299] Note: Compared with the model group, *P < 0.05, **P < 0.01

[0300] 2.5.3 Effect of the present invention on the heat pain threshold of rats with chronic migraine: The heat pain threshold was measured 30 min and 1 h after the last injection of nitroglycerin. Compared with the blank group, the heat withdrawal latency of the model group was significantly shortened (P < 0.05). It was found that compared with the model group, the present invention group effectively prolonged the heat withdrawal latency of rats with chronic migraine, and the difference was statistically significant. See Table 7.

[0301] Table 7: Effect of the present invention on the heat pain threshold of rats with chronic migraine

[0302]

[0303]

[0304]

[0305]

[0306]

[0307]

[0308] Note: Compared with the model group, *P < 0.05, **P < 0.01

[0309] 2.5.4 Effect of the present invention on the 5-HT level in the serum of chronic migraine rats: The 5-HT content was measured 4 h after the last injection of nitroglycerin. The 5-HT level in the serum of rats in the model group was significantly decreased compared with that in the blank group (P < 0.05); compared with the model group, the 5-HT content in the serum of rats in the present invention group was significantly increased, and the difference was statistically significant. It was found that the present invention effectively increased the 5-HT content in the serum of chronic migraine rats. See Table 8.

[0310] Table 8: Effect of the present invention on 5-HT in the serum of chronic migraine rats

[0311]

[0312]

[0313]

[0314]

[0315] Note: Compared with the model group, *P < 0.05, **P < 0.01

[0316] 2.5.5 Effect of the present invention on the CGRP level in the serum of chronic migraine rats: The CGRP content was measured 4 h after the last injection of nitroglycerin. Compared with the blank group, the CGRP level in the serum of rats in the model group was significantly increased, and the difference was statistically significant; it was found that the present invention group could significantly reduce the CGRP level in the serum of chronic migraine rats compared with the model group (P < 0.05). See Table 9.

[0317] Table 9: Effect of the present invention on the CGRP level in the serum of rats

[0318]

[0319]

[0320]

[0321] Note: Compared with the model group, *P < 0.05, **P < 0.01

[0322] Verification Example 3: Acute toxicity experiment

[0323] 1. Materials

[0324] 1.1 Experimental animals: A total of 40 SPF-grade ICR mice, with 20 males and 20 females, with an average body weight of 18.0 - 22.0 g, were all provided by the Animal Experiment Center of Zhejiang Chinese Medical University and were housed separately by gender. Before the formal start of the experiment, all mice were fed adaptively for 7 days. The breeding conditions, drinking water, and feed of the experimental animals met national standards and relevant regulations.

[0325] 1.2 Drugs: The headache-relieving and depression-resolving and collaterals-unblocking granules prepared by the present invention, specification: containing 3.875 g of crude drug per gram.

[0326] 2. Methods: In view of the fact that there are no toxic herbs in this prescription, according to the "Technical Guidelines for Single-dose Toxicity Studies of Drugs" (2015 edition) and relevant test requirements, the maximum tolerated dose (MTD) test was carried out.

[0327] 2.1 Animal grouping: After 7 days of adaptive feeding, the mice were divided into the TTL group (n = 20) and the control group (n = 20) by the random number method, with 10 males and 10 females in each group.

[0328] 2.2 Administration: The headache-relieving and depression-resolving and collaterals-unblocking granules were prepared from the crude drugs of the headache-relieving and depression-resolving and collaterals-unblocking formula through extraction, concentration, inclusion, drying, and granulation. Through preliminary tests, the maximum preparation concentration of the crude drugs of the headache-relieving and depression-resolving and collaterals-unblocking formula was about 30% (3 g of crude drug / ml), and the maximum administration volume was 40 ml / kg. The animals were fasted for 6 h before administration, water was not restricted, and the drug was administered by oral gavage twice a day at an interval of 4 h.

[0329] 2.3 Observation of general status: After the last administration every day, observe and record the general status of the animals, such as symptoms of animal hair color, limb movement, expression, drinking water, excretion, etc., and observe whether there are toxic symptoms. If so, record in detail the toxic symptoms, onset time, duration, and recovery situation, and observe continuously for 14 days.

[0330] 2.4 Body weight observation: Record the body weights of the mice in each group on the 1st day (D1), 2nd day (D2), 3rd day (D3), 5th day (D5), 7th day (D7), 10th day (D10), and 14th day (D14) after administration.

[0331] 2.5 Food intake observation: Add a fixed amount of feed to the mice in each group on the day of administration (D0), and add a fixed amount of feed after weighing the remaining feed on D1, D2, D3, D5, D7, and D10, and weigh the remaining feed on D14 to calculate the average food intake per cage.

[0332] 2.6 Anatomical observation: The hearts, lungs, tracheas, kidneys, bladders, adrenal glands, brains, cerebella, brainstems, spleens, thymuses, testes, epididymides, ovaries, esophagi, stomachs, small intestines, large intestines, livers, submandibular glands, pancreases, and thyroids of mice in each group were carefully dissected and separated in strict accordance with anatomical operation specifications. When changes in the volume, color, texture, etc. of organs were found, the changed organs were fixed with 10% paraformaldehyde, stained with HE, and subjected to histopathological examination. If no obvious abnormalities were observed macroscopically, tissue fixation and histopathological examination were omitted.

[0333] 2.7 Data statistics and analysis: SPSS 25.0 software was used to statistically analyze all the data in this study. Measurement data were expressed in the form of mean ± standard deviation. Chi-square test was used within the group, and corrected t-test was used between groups. P < 0.05 indicated that the difference was statistically significant.

[0334] 3 Results

[0335] 3.1 Observation results under general conditions: In this MTD test, no obvious stress responses were observed in the mice in the TTL group and the control group during oral gavage and observation; there were no obvious changes in appearance, hair color, and limb activities; excreta were normal; no obvious poisoning symptoms appeared, and no deaths were observed in the mice of each group.

[0336] 3.2 Observation results of body weight

[0337] The body weights of mice in each group were recorded on the 1st day (D1), 2nd day (D2), 3rd day (D3), 5th day (D5), 7th day (D7), 10th day (D10), and 14th day (D14) after administration. Compared with the control group, the body weights of male and female mice in the TTL group were slightly decreased during the whole test period. The changes in the body weights of the two groups of mice were positively correlated with the number of test days, that is, as the number of test days extended, the body weights of the two groups of mice both showed an increasing trend, but the changes were not significantly different, and the difference was not statistically significant (p > 0.05). See Table 10.

[0338] Table 10: Effects of Jietongluo Granules for Headache and Depression on the Body Weights of Mice in Acute Oral Toxicity Test (g, )

[0339]

[0340] Note: Compared with the control group, *P < 0.05, **P < 0.01

[0341] 3.3 Results of changes in food intake

[0342] Compared with the control group, the food intake of female mice in the TTL group showed slight fluctuations during the experiment. There was a significant difference in food intake only during the drug administration period from D1 to D2 (the difference was statistically significant), and there was no statistically significant difference at other times. Compared with the control group, the food intake of male mice in the TTL group also showed slight fluctuations during the entire experiment, but there was no significant difference (P>0.05). See Table 11.

[0343] Table 11: Effects of acute oral toxicity test of Tongtou Jieyu Tongluo Granules on food intake of mice (g, )

[0344]

[0345]

[0346] Note: Compared with the control group, *P<0.05, **P<0.01

[0347] 3.4 Anatomical examination results

[0348] The colors, volumes, and textures of the organs such as the heart, liver, spleen, lungs, and kidneys of the mice in each group were normal, and no obvious pathological changes or abnormal alterations were observed in the important organs by macroscopic observation.

[0349] 4 Conclusion: Tongtou Jieyu Tongluo Granule is composed of 7 herbs including Ligusticum chuanxiong. The formula is concise, the compatibility is scientific, and the curative effect is remarkable. The proposed daily dosage of crude drugs for clinical adults (calculated based on a body weight of 60 kg) is 1.55 g / kg. Through preliminary experiments, the maximum preparation concentration of the crude drugs in the Tongtou Jieyu Tongluo formula was approximately 30% (3 g of crude drugs / ml), the maximum administration volume was 40 ml / kg, twice a day. The cumulative content of crude drugs administered to ICR mice by oral gavage daily was 240 g / kg, which is 155 (240 g / 1.55 g ≈ 154.84) times the clinical dosage.

[0350] Based on this, no obvious acute poisoning symptoms occurred at 155 times the clinical application dose of Tongtou Jieyu Tongluo Granule. It can be concluded that it has no obvious toxicity under high-dose application, and the clinical dosage is safe and non-toxic.

Claims

1. A Chinese medicine composition for treating chronic headache, characterized in that: The Chinese medicine composition is composed of the following components in parts by weight: 1200-1300 parts of Chuanxiong, 570-670 parts of white peony root, 450-550 parts of Pueraria root, 60-500 parts of vinegar Corydalis, 330-420 parts of honey bran Bombyx batryticatus, 320-420 parts of vinegar Cyperus rotundus, and 200-300 parts of Angelica dahurica.

2. The method for preparing a Chinese medicine composition for treating chronic headache according to claim 1, characterized in that The following steps are involved: 1) Take the formulated amount of Chuanxiong and vinegar-cured Cyperus rotundus and soak them in 5 times the amount of water as a solvent, extract them by steam distillation, collect the volatile oil, and wrap the volatile oil with a saturated β-cyclodextrin solution for later use; 2) centrifuging the extracted drug solution in step 1) to remove impurities and set aside; 3) taking the residue after the volatile oil extraction in step 1), adding the formulated amount of white peony root, kudzu root and honey bran silkworm, using 10 times the amount of water as solvent, reflux extraction three times, each time for 1 hour, combining the extracts, centrifuging the extracts to remove impurities, and concentrating them into a first clear paste; 4) Grind the vinegar Corydalis slices and pass them through a No. 3 sieve, take the vinegar Corydalis powder and Angelica dahurica in the formula amount, use 7 times the amount of 60% ethanol as solvent, reflux extract twice, each time for 1.5 hours, combine the extracts, remove impurities with a plate and frame filter, and concentrate into the second clear paste; 5) The first clear paste and the second clear paste are combined, spray-dried to obtain dry powder, and the volatile oil encapsulated by the β-cyclodextrin solution obtained in step 1) is added, and auxiliary materials are added to obtain granules by dry method.

3. A method for preparing a Chinese medicine composition for treating chronic headache as claimed in claim 2, characterized in that In step 1), the mixture is immersed for 0.5 h and steam distilled for 9.5 h.

4. A method for preparing a Chinese medicine composition for treating chronic headache as claimed in claim 2, characterized in that In step 1), the specific steps of encapsulating the volatile oil with a saturated β-cyclodextrin solution are as follows: taking volatile oil: ethanol at a volume ratio of 1:4 to prepare an ethanol solution of volatile oil; taking β-CD at a solid-liquid ratio of 1g:10mL, adding water at 60°C and stirring for 10min, cooling to room temperature to prepare a saturated β-CD solution, and then transferring it to a constant temperature magnetic stirrer, adding the ethanol solution of volatile oil dropwise under stirring at a volume ratio of β-cyclodextrin: volatile oil = 8:1, the magnetic stirring speed is 300 r / min, the temperature is 40°C, the inclusion is carried out for 180min to a suspended state, cooling to room temperature and refrigerating in a refrigerator for 24h, then filtering, washing once with pure water, and the obtained inclusion complex is naturally dried to obtain a dry inclusion complex.

5. The method for preparing a Chinese medicine composition for treating chronic headache according to claim 2, characterized in that In step 3), the relative density of the first clear paste is 1.10±0.02 g / cm 3 , the concentration temperature is 40-50℃.

6. The method for preparing a Chinese medicine composition for treating chronic headache according to claim 2, characterized in that In step 4), the reflux extraction temperature is 85°C, and the second clear paste The relative density is 1.00±0.02 g / cm 3 , the concentration temperature is 40- 50℃。 7. The method for preparing a Chinese medicine composition for treating chronic headache according to claim 2, characterized in that In step 5), the spray drying conditions are as follows: air inlet temperature 178°C, creep speed 12 rpm, relative density of the drug solution 1.07 g / cm 3 .

8. The method for preparing a Chinese medicine composition for treating chronic headache according to claim 2, characterized in that In step 5), the auxiliary materials are dextrin and magnesium stearate, the added amount of dextrin accounts for 5% of the total amount, and the added amount of magnesium stearate accounts for 0.5% of the total amount.

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