Contact lens care solution and care method

Abstract

The invention relates to a contact lens care solution and a care method. The contact lens care solution comprises the following materials in percentage by weight: 0.1-2wt% of potassium bromide, 0.05-0.5 wt% of a pH buffer agent, 0.01-0.1 wt% of a polysaccharide compound, 0.005-0.02 wt% of protein microspheres and the balance of water, the pH value of the care solution is 9-11; the contact lens care method comprises the following steps: adding the contact lens care solution into an electrolysis device, immersing a to-be-cleaned contact lens into the care solution, electrifying for electrolytic cleaning, and washing the lens with normal saline before wearing; according to the care solution and the care method thereof, the contact lenses can be disinfected, cleaned, deproteinized, moisturized, rubbing-free, scratch-free and deformation-free; and the tear protein cleaning removal rate reaches 97% or above.

Description

Technical Field

[0001] The present invention relates to the technical field of contact lens care solutions, and particularly relates to a contact lens care solution and a care method. Background Art

[0002] Contact lens care solutions were previously divided into three types: disinfection solutions, lens cleaning solutions, and comfort solutions. However, they were rather inconvenient to use. After removing the lenses, first drop the cleaning solution on the lenses, rub the lenses with fingers, rinse, and then soak the lenses in the disinfection solution for storage. After wearing the lenses the next day, according to the discomfort of the eyes, the comfort solution is dropped irregularly. This previous care method was not convenient enough. If the labels or packages of the three care solutions were similar, it was very easy to make mistakes; in addition, the protein and lipid contaminants adhered to the contact lenses that were not cleaned thoroughly would bring the risk of inflammation and a decrease in comfort.

[0003] With the continuous upgrading of contact lens lenses and the increasing variety of lens types, multifunctional contact lens care solutions have emerged. Such care solutions integrate the functions of lens cleaning, disinfection, and comfort. However, the procedures for using and maintaining the lenses are still the same as before, and there are still problems such as incomplete cleaning, especially poor protein removal effect. In addition, some products on the market have added many other functions in addition to the basic functions. For the effects of other components, it must be based on science and cannot be blindly selected and added. The addition of inappropriate components may even affect the efficacy of the basic components, thereby affecting the lens care effect.

[0004] Currently, there is a phenomenon among contact lens wearers of "emphasizing selection and neglecting care". Most of them only use the method of "soaking in the care solution" for cleaning, without adding manual rubbing, and claim that "manual rubbing is very troublesome". However, even if rubbing is added, it cannot effectively remove the tear protein, especially the tear protein in the oxygen-permeable pores of the lenses; in addition, rubbing will inevitably cause different degrees of wear, scratches, and deformation of the lenses.

[0005] Therefore, how to disinfect, clean, remove protein, moisturize, avoid scratches, and avoid deformation of contact lenses is the technical problem to be solved by the present invention. Summary of the Invention

[0006] In order to solve the above technical problems, a contact lens care solution and a care method are provided. The method of the present invention can achieve the effects of disinfecting, cleaning, removing protein, moisturizing, avoiding rubbing, avoiding scratches, and avoiding deformation of contact lenses.

[0007] To achieve the above object, the present invention is realized through the following technical solutions:

[0008] A contact lens care solution, comprising the following materials in 100% by weight percentage: potassium bromide 0.1 - 2 wt%, pH buffer 0.05 - 0.5 wt%, polysaccharide compound 0.01 - 0.1 wt%, protein microspheres 0.005 - 0.02 wt%, and the balance is water; the pH of the care solution is 9 - 11.

[0009] Preferably, the contact lens care solution comprises the following materials in 100% by weight percentage: potassium bromide 0.5 - 1 wt%, pH buffer 0.05 - 0.1 wt%, polysaccharide compound 0.01 - 0.05 wt%, protein microspheres 0.01 wt%, and the balance is water.

[0010] Further, the pH buffer is one of carbonates and phosphates; the carbonates are selected from one or more of sodium carbonate, sodium bicarbonate, potassium carbonate, and potassium bicarbonate; the phosphates are selected from one or more of sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium hydrogen phosphate, and potassium dihydrogen phosphate; if multiple are selected, then multiple carbonates, phosphates, or borates with the same metal cation are selected in the same series.

[0011] Further, the polysaccharide compound is selected from one or more of chitosan or its salts, chitooligosaccharide or its salts, hyaluronic acid or its salts; the molecular weight of the chitosan or its salts is 500KDa - 700KDa, and the degree of deacetylation is not less than 10%; the molecular weight of the chitooligosaccharide or its salts is 320Da - 3200Da; the molecular weight of the hyaluronic acid or its salts is less than 1.5 million Da.

[0012] Preferably, the polysaccharide compound is a combination of one of chitosan or its salts, chitooligosaccharide or its salts and hyaluronic acid or its salts.

[0013] Further, the protein microspheres are collagen microspheres and / or fibroin microspheres, and the particle size of the microspheres is 20 - 100 μm; the molecular weight of the collagen microspheres is less than 150KDa; the molecular weight of the fibroin microspheres is less than 250KDa; preferably, the molecular weight of the collagen microspheres is 130KDa; the molecular weight of the fibroin microspheres is 100 - 230KDa.

[0014] A contact lens care method, comprising the following steps:

[0015] Add the above-mentioned contact lens care solution to an electrolysis device, immerse the contact lenses to be cleaned in the care solution for 1 - 30 min, then energize for electrolytic cleaning for 5 - 20 min, and then cut off the power after cleaning and can be placed in it for static soaking, and rinse with physiological saline before wearing.

[0016] The electrolysis device at least includes a positive electrode, a negative electrode, a lens placement frame, a cavity for containing the nursing solution, and a power switch. The lens placement frame needs to be immersed in the nursing solution within the cavity.

[0017] Further, the operating power of the electrolysis device is 0.5 - 5W.

[0018] Beneficial technical effects:

[0019] The contact lens nursing solution of the present invention contains polyelectrolytes. For example, chitosan or chitooligosaccharide has positively charged cations, and hyaluronic acid has negatively charged groups, which have an electrostatic sweeping effect on the charged tear proteins on the lens (the main components of tear proteins are lysozyme, albumin, and globulin, and albumin is negatively charged, while lysozyme and globulin are positively charged), and a certain degree of entrapment of tear proteins is achieved. After electrolysis by applying an electric current, the entrapped tear proteins can be quickly attracted to the electrodes under the action of the electric field force and decomposed. The protein microspheres move on the lens surface under the action of the electric field force, playing a scrubbing role. At the same time, potassium bromide in the nursing solution is electrolyzed to produce bromine. After bromine dissolves in water, it not only shows color but also has a good bactericidal and disinfection effect on the lens, and further has an oxidation effect on the tear proteins in the air holes, and then the tear proteins are further decomposed. After nursing contact lenses with the nursing solution of the present invention, the bactericidal and protein-removing effect can reach more than 97%, and the oxygen permeability of the lens can be restored to the original value. The method of the present invention can achieve the effects of disinfecting, cleaning, removing proteins, moisturizing, avoiding rubbing, avoiding scratching, and avoiding deformation of contact lenses. Description of the Drawings

[0020] Figure 1 It is a schematic structural diagram of the electrolysis device. Detailed Embodiments

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments and drawings of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and in no way constitutes a limitation on the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0022] Unless otherwise specifically stated, the numerical values set forth in these embodiments do not limit the scope of the present invention. Technologies and methods known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, such technologies and methods should be regarded as part of the specification. In all examples shown and discussed herein, any specific value should be construed as merely exemplary and not as a limitation. Therefore, other examples of the exemplary embodiments may have different values.

[0023] In the following examples, the experimental methods without specific conditions are usually determined according to national standards; if there is no corresponding national standard, they are carried out according to general international standards or the standard requirements proposed by relevant enterprises. Unless otherwise specified, all parts are by weight, and all percentages are by weight percentage.

[0024] Preparation Example 1

[0025] Establish a detection standard curve for the concentration of lysozyme solution:

[0026] (i) First, prepare a stock solution of artificial protein tear fluid with a concentration of 2.2 mg / mL;

[0027] (ii) Dilute the stock solution to obtain a diluted protein solution with a concentration of 1 mg / mL;

[0028] (iii) Respectively take 0.04 mL, 0.2 mL, 0.4 mL, 0.8 mL, 1.6 mL of the diluted protein solution into corresponding containers, add physiological saline to 4 mL, and prepare working protein solutions with concentrations of 0.01 mg / mL, 0.05 mg / mL, 0.1 mg / mL, 0.2 mg / mL, 0.4 mg / mL;

[0029] (iiii) Detect the absorbance value OD corresponding to the concentration of the above 5 concentrations of working protein solutions at 281 nm with an ultraviolet spectrophotometer 281nm .

[0030] The specific results are shown in Table 1 below.

[0031] Table 1 Relationship between the concentration of the working protein solution and its absorbance value

[0032]

[0033] Preparation Example 2

[0034] Preparation of contact lenses to be cleaned: Take unworn contact lens lenses and place them in multiple sample bottles respectively. Add 2 mL of protein solution with a concentration of 2.2 mg / mL to each sample bottle (a total of 4.4 mg of protein). Incubate the sample bottles with the lenses on a shaker at 37 °C and 100 rpm for 16 h, then take out the lenses and rinse them in physiological saline for 5 s. Use ultraviolet spectrophotometry to detect the absorbance value of the remaining protein in the sample bottles, and calculate the protein adsorption amount on the lenses. 7 parallel experiments of contact lenses to be cleaned, respectively labeled as G1, G2, G3, G4, G5, G6, G7.

[0035] The absorbance of the remaining protein solution in each sample vial was measured after diluting it 10 times. The measured absorbance values of G1, G2, G3, G4, G5, G6, and G7 were 0.3326, 0.3349, 0.3259, 0.3225, 0.3394, 0.3285, and 0.3282, respectively. Calculate the remaining protein concentration and content in the sample vial, as shown in Table 2 below.

[0036] Table 2 Protein content adsorbed on the lenses of G1 - G7

[0037]

[0038] (Note: When calculating the remaining protein concentration in the vial according to Equation Ⅰ, the absorbance value data in the previous column needs to be multiplied by 10 before calculation; when calculating the protein content adsorbed on the lens, multiply the remaining protein concentration in the previous column by 2 mL to obtain the remaining amount in the vial, and subtract it from the total protein amount of 4.4 mg.)

[0039] The clearance rate (denoted as C) of the protein on the lens by the following nursing solution in the following examples is calculated according to Equation Ⅱ:

[0040] Equation Ⅱ: C = (the amount of protein removed by the nursing solution / the amount of protein adsorbed on the lens) × 100%.

[0041] Example 1

[0042] A contact lens nursing solution, comprising the following materials in 100% by weight: 1.5% potassium bromide, 0.05% sodium carbonate, 0.01% chitosan with a deacetylation degree of not less than 10% and a molecular weight of 500 - 700 kDa, 0.01% hyaluronic acid with a molecular weight of less than 1.5 million Da, 0.01% silk fibroin microspheres with a particle size of 20 - 100 μm and a molecular weight of 100 kDa, and the balance being purified water;

[0043] Among them, chitosan and hyaluronic acid are purchased from Shanghai Haoleyuan Biotechnology Co., Ltd.;

[0044] Among them, the preparation of silk fibroin microspheres:

[0045] (i) Take 20 g of silk, wash it and place it in 1.0 L of 0.05 wt% sodium carbonate solution and boil for 30 min. After fishing out the silk and washing it with deionized water, repeat the boiling step and washing once to obtain degummed silk fibroin; dissolve the degummed silk in 80 mL of 9.3 M lithium bromide solution to obtain a silk fibroin protein mixed solution, and put this mixed solution into a dialysis bag with a cut-off molecular weight of 100 kDa and dialyze it in deionized water for 60 hours to obtain a pure silk fibroin protein solution;

[0046] (ii) Prepare an oil phase solution: 60 wt% oleic acid, 15 wt% polyglycerol monostearate, 25 wt% ethanol;

[0047] (iii) Disperse the obtained silk fibroin solution into the oil phase solution to form a dispersion with microdroplet diameters of 20 - 100 μm. The volume ratio of the oil phase solution to the silk fibroin solution is 4:1. Then, let the dispersion stand in a refrigerator at 3°C for 12 h for solidification, and then rinse and filter with medical alcohol to obtain silk fibroin microspheres.

[0048] A method for caring for contact lenses, comprising the following steps:

[0049] Adopt an electrolysis device as Figure 1 shown. The electrolysis device includes a positive electrode, a negative electrode, a lens placement frame connected to the lid, a cavity for accommodating the care solution, and a switch. It also includes a care solution filling warning line, a magnetic suction power supply for power supply, and a plug. The connector can adopt a USB plug, and the lens placement frame needs to be immersed in the care solution in the cavity;

[0050] Use: Add 4 mL of the care solution with the above components to the electrolysis device. Place the contact lens to be cleaned (G1) in the lens placement frame. After tightening the lid, the contact lens to be cleaned (G1) is immersed in the care solution. After 5 min of immersion, power on and perform electrolysis cleaning at a power of 5 W for 5 min. After power off, rinse the lens with physiological saline.

[0051] Detect the absorbance of the care solution after the above soaking. The specific results are shown in Table 3.

[0052] Example 2

[0053] A contact lens care solution, comprising the following materials in 100% by weight: 1.5% potassium bromide, 0.1% sodium carbonate, 0.04% chitosan with a molecular weight of 320 - 3200 Da, 0.01% hyaluronic acid with a molecular weight less than 1.5 million Da, 0.01% collagen microspheres with a size of 20 - 100 μm and a molecular weight of 130 kDa, and the balance being purified water;

[0054] The preparation process of the collagen microspheres: First, dissolve the collagen purchased from Nitta Gelatin Co., Ltd. in hot water to form a 15 g / L collagen solution, and the subsequent operations are the same as steps (ii) and (iii) in Example 1.

[0055] A method for caring for contact lenses, comprising the following steps:

[0056] In the electrolysis device as Figure 1 shown, add 4 mL of the care solution with the above components. Immerse the contact lens to be cleaned (G2) in the care solution for 10 min, then power on and perform electrolysis cleaning at a power of 5 W for 10 min. After power off, rinse the lens with physiological saline.

[0057] The absorbance of the above-mentioned soaked nursing solution was detected, and the specific results are shown in Table 3.

[0058] Example 3

[0059] A contact lens nursing solution comprises the following materials in 100% by weight: 1.5% potassium bromide, 0.05% sodium carbonate, 0.02% chitosan with a degree of deacetylation of not less than 10% and a molecular weight of 500 - 700 kDa, 0.01% hyaluronic acid with a molecular weight of less than 1.5 million Da, 0.01% collagen microspheres with a particle size of 20 - 100 μm and a molecular weight of 130 kDa (the same microspheres as those used in Example 2), and the balance is purified water.

[0060] A contact lens nursing method comprises the following steps:

[0061] In the electrolysis device as Figure 1 shown, 4 mL of the nursing solution with the above components was added, the contact lens to be cleaned (G3) was immersed in the nursing solution for 20 min, then powered on, and electrolytic cleaning was carried out at a power of 1 W for 20 min. After power-off, the lens was rinsed with physiological saline.

[0062] The absorbance of the above-mentioned soaked nursing solution was detected, and the specific results are shown in Table 3.

[0063] Comparative Example 1

[0064] The nursing solution formulation of this comparative example comprises the following materials in 100% by weight: 1.5% potassium bromide, 0.1% sodium carbonate, and the balance is purified water. The contact lens to be cleaned was G4, and the cleaning process was the same as that in Example 1. The absorbance of the above-mentioned soaked nursing solution was detected, and the specific results are shown in Table 3.

[0065] Comparative Example 2

[0066] The nursing solution formulation of this comparative example was the same as that in Example 1, except that chitosan was absent. The contact lens to be cleaned was G5, and the cleaning process was the same as that in Example 1. The absorbance of the above-mentioned soaked nursing solution was detected, and the specific results are shown in Table 3.

[0067] Comparative Example 3

[0068] The nursing solution formulation of this comparative example was the same as that in Example 1, except that silk fibroin microspheres were absent. The contact lens to be cleaned was G6, and the cleaning process was the same as that in Example 1. The absorbance of the above-mentioned soaked nursing solution was detected, and the specific results are shown in Table 3.

[0069] Comparative Example 4

[0070] The care solution formulation of this comparative example is the same as that of Example 1, except that there is no hyaluronic acid. The contact lenses to be cleaned are G7, and the cleaning process is the same as that of Example 1. The absorbance of the above-mentioned soaked care solution was detected, and the specific results are shown in Table 3.

[0071] Table 3 Cleaning effects of examples and comparative examples

[0072]

[0073] (Note: When calculating the protein concentration in the care solution after cleaning according to Equation I, the absorbance value data in the previous column needs to be multiplied by 2 before calculation; when calculating the protein content in the care solution after cleaning, multiply the protein concentration in the previous column by 4 mL to obtain the protein content, and the clearance rate in the last column can be obtained by calculating according to Equation II)

[0074] As can be seen from Table 3, when the care solution of the present invention is used to clean the lens, first soak for 5 - 20 minutes, and then clean by electrolysis with electricity for 5 - 20 minutes, and then the tear protein can be basically cleaned. In Comparative Example 1, there are only potassium bromide and sodium carbonate, and the clearance rate of this care solution for tear protein is less than 50%. In Comparative Example 2, protein microspheres and hyaluronic acid were added on the basis of the components of Comparative Example 1, and the clearance rate of this care solution for tear protein can reach 70.42%; in Comparative Example 3, chitosan and hyaluronic acid were added on the basis of the components of Comparative Example 1, and the clearance rate of this care solution for tear protein can reach 80%; in Comparative Example 4, chitosan and protein microspheres were added on the basis of the components of Comparative Example 1, and the clearance rate of this care solution for tear protein is less than 70%; while the care solution formed by adding 0.01 - 0.04% of chitosan or chitooligosaccharide, 0.01% of hyaluronic acid, and 0.01% of protein microspheres on the basis of the basic components of the present invention can achieve a clearance effect of more than 97% on tear protein.

[0075] The above is only the preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A contact lens care solution, characterized in that, It comprises the following materials in 100% by weight: potassium bromide 0.1 - 2 wt%, pH buffer 0.05 - 0.5 wt%, polysaccharide compound 0.01 - 0.1 wt%, protein microspheres 0.005 - 0.02 wt%, and the balance is water; the pH of the nursing solution is 9 - 11.

2. The contact lens care solution according to claim 1, characterized in that, The contact lens nursing solution comprises the following materials in 100% by weight: potassium bromide 0.5 - 1 wt%, pH buffer 0.05 - 0.1 wt%, polysaccharide compound 0.01 - 0.05 wt%, protein microspheres 0.01 wt%, and the balance is water.

3. The contact lens care solution according to claim 1 or 2, characterized in that, The pH buffer is one of carbonate and phosphate; the carbonate is selected from one or more of sodium carbonate, sodium bicarbonate, potassium carbonate, and potassium bicarbonate; the phosphate is selected from one or more of sodium hydrogen phosphate, sodium dihydrogen phosphate, potassium hydrogen phosphate, and potassium dihydrogen phosphate.

4. A contact lens care solution according to claim 1 or 2, characterized in that, The polysaccharide compound is selected from one or more of chitosan or its salt, chitosan oligosaccharide or its salt, hyaluronic acid or its salt; the molecular weight of chitosan or its salt is 500KDa - 700KDa and the deacetylation degree is not less than 10%; the molecular weight of chitosan oligosaccharide or its salt is 320Da - 3200Da; the molecular weight of hyaluronic acid or its salt is less than 1.5 million Da.

5. The contact lens care solution according to claim 4, characterized in that, The polysaccharide compound is a combination of one of chitosan or its salt, chitosan oligosaccharide or its salt and hyaluronic acid or its salt.

6. The protein microspheres are collagen microspheres and / or fibroin microspheres, and the particle size of the microspheres is 20 - 100 μm; the molecular weight of the collagen microspheres is less than 150KDa; the molecular weight of the fibroin microspheres is less than 250KDa.

7. A contact lens care method, characterized in that, It comprises the following steps: Add the contact lens nursing solution as described in any one of claims 1 - 6 into the electrolysis device, immerse the contact lens to be cleaned in the nursing solution for 1 - 30 min, then conduct electrolysis cleaning for 5 - 20 min to complete the cleaning, and rinse the lens with physiological saline before wearing.

8. A contact lens care method according to claim 7, wherein, The working power of the electrolysis device is 0.5 - 5W.

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