A dandruff composition for men with sensitive skin and application thereof

Abstract

The application discloses a dandruff-removing composition for men with sensitive skin and application, and relates to the technical field of washing and protecting products.The application provides a dandruff-removing composition, which comprises the following components in parts by weight: Picea abies leaf extract 0.5-5 parts, Hypericum perforatum extract 0.1-2 parts, Salvia miltiorrhiza extract 0.1-2 parts and Olea europaea leaf extract 0.5-5 parts; the Salvia miltiorrhiza extract is a mixture of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract.The composition prepared by the application can repair the scalp homeostasis from three aspects of fungus proliferation inhibition, sebum secretion regulation and barrier function repair, so that the effect of better controlling oil and reducing dandruff is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of toiletries, in particular to an anti-dandruff composition suitable for men with sensitive skin and its application. Background Art

[0002] In the field of men's personal care, dandruff caused by scalp microecological imbalance has become a core pain point affecting consumer experience. Currently available men's anti-dandruff shampoos generally have the following technical defects: (1) The anti-dandruff active ingredient has a single target, mainly relying on antifungal agents such as zinc pyrithione and ketoconazole, which only inhibit Malassezia and fail to effectively intervene in the synergistic pathogenic links such as sebum secretion and barrier repair; (2) The proportion of chemical additives in the formula system is too high, causing the product pH value to deviate from the normal range of the scalp, and long-term use can easily damage the lipid structure of the stratum corneum; (3) There is a lack of a scalp microecological balance regulation mechanism. Existing technologies mostly use broad-spectrum sterilization methods, which significantly reduce the abundance of symbiotic bacteria while inhibiting Malassezia, but aggravate the imbalance of the microbial community.

[0003] From the perspective of pathological mechanism, the formation of dandruff is the result of the synergistic action of multiple pathogenic factors: First, in terms of microecological imbalance, Malassezia secretes lipase to hydrolyze triglycerides in sebum into pro-inflammatory unsaturated fatty acids, which can penetrate the stratum corneum of the scalp, causing scalp itching while further leading to excessive keratinization of scalp keratinocytes and accelerating the production of dandruff. Secondly, the androgen sensitivity unique to men leads to a high secretion state of sebaceous glands, which directly stimulates an increase in the rate of sebum secretion. Furthermore, the disorder of the scalp barrier function forms a vicious circle. When the expression of epidermal permeability barrier-related proteins (such as filaggrin and loricrin) is downregulated, the transepidermal water loss rate (TEWL) increases and the barrier is damaged.

[0004] Therefore, developing an anti-dandruff composition that conforms to the physiological characteristics of the scalp and can maintain the balance of microecology has become a technical problem that needs to be solved urgently in this field. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a men's anti-dandruff composition and application suitable for sensitive skin, which has a triple action mechanism of inhibiting fungal proliferation, regulating sebum secretion, and repairing barrier function.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: an anti-dandruff composition, comprising the following components in parts by weight: 0.5-5 parts of Norway spruce leaf extract, 0.1-2 parts of Hypericum perforatum extract, 0.1-2 parts of Salvia miltiorrhiza extract, and 0.5-5 parts of olive leaf extract; the Salvia miltiorrhiza extract is a mixture of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract.

[0007] The Norway spruce leaf extract contains rich polysaccharides, flavones and polyphenols, can inhibit the growth of fungi and has certain anti-inflammatory effect, and can inhibit the secretion of lipids by sebaceous gland cells and weaken the influence of excessive secretion of sebum on dandruff. The Hypericum perforatum extract contains flavones, flavonols and flavanones, which can interfere with the synthesis of Malassezia cell membrane, destroy the structure of the fungal cell membrane by reducing the production of ergosterol, and thus inhibit the growth of Malassezia; at the same time, the Hypericum perforatum extract can affect the proliferation and differentiation of keratinocytes, thereby enhancing the skin barrier function. The components such as tanshinone and salvianolic acid in the Salvia miltiorrhiza extract enhance the anti-inflammatory effect of the composition; at the same time, the activity of 5α-reductase can be inhibited, and the excessive secretion of sebum induced by DHT can be reduced. The Olea europaea leaf extract can destroy the integrity of the fungal cell membrane and inhibit the growth of mycelium; can regulate the differentiation of sebaceous gland cells and reduce the expression of lipid synthesis-related genes; at the same time, the oleuropein in the Olea europaea leaf extract can up-regulate the expression of filaggrin and enhance the water-retention capacity of the stratum corneum.

[0008] The inventors found in actual experiments that the Norway spruce leaf extract, the Hypericum perforatum extract, the Salvia miltiorrhiza extract and the Olea europaea leaf extract can be used together to prepare a composition which can repair the scalp homeostasis from the aspects of fungal proliferation inhibition, sebum secretion regulation and barrier function repair, thereby achieving better control of oil and reduction of dandruff. It is also found in actual experiments that the product prepared from the composition of the present application can better maintain the microecological balance, and the problem of dandruff will not recur after the product is discontinued.

[0009] Preferably, the anti-dandruff composition comprises the following components by weight: 1-3 parts of Norway spruce leaf extract, 0.5-1 part of Hypericum perforatum extract, 0.5-1 part of Salvia miltiorrhiza extract and 1-2 parts of Olea europaea leaf extract.

[0010] Preferably, the weight percentage of the Salvia miltiorrhiza extract in the total weight of the anti-dandruff composition is 15-25%.

[0011] The inventors found in actual research that the weight of the components in the anti-dandruff composition provided in the present application can affect the performance of the product, and when the weight of the components is further selected within the above range, the product obtained has better effects of inhibiting fungal proliferation, regulating sebum secretion and repairing barrier function.

[0012] Preferably, the weight ratio of the Salvia miltiorrhiza root extract to the Salvia miltiorrhiza leaf extract is 1:(1-3).

[0013] The polyphenol components in the Salvia miltiorrhiza root extract can inhibit the catalysis of Malassezia on the synthesis of HCO3 -The invention can inhibit the activity of β-carbonic anhydrase, a key enzyme in the fight against Malassezia; in addition, the root extract of Salvia miltiorrhiza also inhibits the excessive differentiation of sebaceous gland cells; the flavonoids and phenolic acids in the leaf extract of Salvia miltiorrhiza have excellent anti-inflammatory and antioxidant effects. The inventors found in actual experiments that when the Salvia miltiorrhiza extract is a mixture of a root extract of Salvia miltiorrhiza and a leaf extract of Salvia miltiorrhiza, it is more effective in controlling oil and reducing dandruff. The inventors found in actual research that when the weight ratio of the root extract of Salvia miltiorrhiza to the leaf extract of Salvia miltiorrhiza is within the above range, it is more effective in controlling oil and reducing dandruff.

[0014] Preferably, the Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract can be commercially available products or homemade.

[0015] Preferably, the preparation method of the Salvia miltiorrhiza root extract comprises the following steps:

[0016] S1, taking dried, crushed and sieved Salvia miltiorrhiza root, adding it to deionized water, adjusting the pH to 3-4, adding a complex enzyme, wherein the complex enzyme includes a mixture of cellulase, pectinase and acid protease, and collecting the enzymatic hydrolysis product after enzymolysis;

[0017] S2. Adding an organic solvent to the enzymatic hydrolyzate for ultrasonic extraction, filtering, collecting the filtrate, and rotary evaporating the filtrate and drying it to obtain the Salvia miltiorrhiza root extract.

[0018] Preferably, the weight ratio of the complex enzyme to the root of Salvia miltiorrhiza is 1:(2-4), and the weight ratio of cellulase, pectinase and acid protease in the complex enzyme is cellulase:pectinase:acid protease=(1-3):(0.5-2):(0.1-0.5);

[0019] And / or, the organic solvent is an ethanol solution with a volume fraction of 60-70%, and the weight ratio of the enzymatic hydrolysate to the ethanol solution is 1:(20-40);

[0020] and / or, the mesh size of the sieve is 800-1000 mesh, and the weight ratio of the salvia miltiorrhiza root to deionized water is 1:(10-20);

[0021] And / or, the enzymatic hydrolysis temperature is 40-45° C., the enzymatic hydrolysis time is 20-30 min; after the enzymatic hydrolysis, the enzymatic hydrolysis product is collected by centrifugation at a speed of 3000-5000 r / min;

[0022] And / or, the temperature of the ultrasonic extraction is 50-60°C, the time of the ultrasonic extraction is 15-30 minutes, and the ultrasonic power is 60-75w;

[0023] And / or, the rotary evaporation temperature is 40-50°C;

[0024] And / or, the drying condition is vacuum drying at 50-60° C. for 12-48 hours.

[0025] And / or, the enzymatic activity of the cellulase is 20-50 U / mg, the enzymatic activity of the pectinase is 20-50 U / mg, and the enzymatic activity of the acid protease is 30-80 U / mg.

[0026] Preferably, the preparation method of the Salvia miltiorrhiza leaf extract comprises the following steps:

[0027] (1) drying and crushing the Salvia miltiorrhiza leaves to obtain Salvia miltiorrhiza leaf powder; the mesh size of the Salvia miltiorrhiza leaf powder is 40-50 mesh;

[0028] (2) Extracting the Danshen leaf powder with 8-10 times the amount of ethanol solution, collecting the filtrate, rotary evaporating and drying to obtain the Danshen leaf extract.

[0029] Preferably, the volume fraction of the ethanol solution is 60-70%;

[0030] And / or, the extraction is ultrasonic extraction, the ultrasonic extraction temperature is 55-65° C., the ultrasonic extraction time is 1-2 h, and the ultrasonic extraction power is 60-70 W;

[0031] And / or, the rotary evaporation temperature is 40-50°C;

[0032] And / or, the drying condition is vacuum drying at 50-60° C. for 12-48 hours.

[0033] In actual research, the inventors found that when the Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract obtained by the above preparation method are mixed and used, the anti-dandruff composition prepared can be applied to the final washing and care product to better achieve the effects of inhibiting fungal proliferation, regulating sebum secretion, and repairing barrier function.

[0034] In addition, the present invention provides the use of the anti-dandruff composition in the preparation of toiletries.

[0035] Furthermore, the present invention provides a hair care product, which includes the anti-dandruff composition. The hair care product includes any one of shampoo and conditioner.

[0036] In addition, the present invention provides a shampoo comprising the following components in percentage by mass: 1-8% of the anti-dandruff composition of the present invention, 0.7-1.5% of a thickener, 15-20% of a surfactant, 0.7-1% of a preservative, 0.1-0.2% of a chelating agent, 0.1-0.5% of a pH regulator, and the balance being water.

[0037] Preferably, the thickener comprises at least one of sodium chloride, potassium chloride and hydroxypropyl methylcellulose.

[0038] Preferably, the surfactant includes at least one of polyquaternium-10, cocamidopropyl betaine, potassium cocoyl hydrolyzed oat protein, sodium methyl cocoyl taurate, cocoyl glucoside, sodium C14-16 olefin sulfonate, polyquaternium-7, and capryloyl glycine.

[0039] Preferably, the preservative comprises at least two of sodium benzoate, phenoxyethanol, and ethylhexylglycerin. Specifically, the mass percentage of the sodium benzoate is less than 0.5%, and the mass percentage of the phenoxyethanol is less than 1%.

[0040] Preferably, the chelating agent is disodium EDTA.

[0041] Preferably, the pH adjuster is citric acid.

[0042] Compared to existing technologies, the present invention offers the following benefits: The inventors discovered during actual experiments that Norway spruce leaf extract, Hypericum perforatum extract, Salvia miltiorrhiza extract, and Olea europaea leaf extract synergize to create a composition that can improve scalp homeostasis by inhibiting fungal proliferation, regulating sebum secretion, and repairing barrier function, achieving better oil control and reducing dandruff. Furthermore, during actual experiments, it was found that products prepared using the present composition can better maintain microecological balance, preventing recurrence of dandruff after discontinuation of product use. DETAILED DESCRIPTION

[0043] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials.

[0044] The raw materials used in the present invention are now further described, but are not limited to the following raw materials:

[0045] Norway spruce leaf extract: Norway spruce (PICEA EXCELSA) leaf extract, purchased from Norture Lights Cell Science Inc., Canada;

[0046] Hypericum perforatum extract: purchased from Laina Biotechnology;

[0047] Olive leaf extract: purchased from Draco;

[0048] Tremella fuciformis extract: purchased from Jiayu Health Technology (Zhejiang) Co., Ltd.

[0049] Forsythia suspensa extract: purchased from Shaanxi Guanyiyuan Biotechnology Co., Ltd.

[0050] Olive fruit extract: purchased from Fufeng Ciyuan Biotechnology Co., Ltd.

[0051] Cellulase: enzyme activity 30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.;

[0052] Pectinase: enzyme activity 30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.;

[0053] Acidic protease: enzyme activity 50 U / mg, purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0054] Salvia miltiorrhiza root extract A: Homemade, preparation method is as follows:

[0055] S1. Dried and crushed Salvia miltiorrhiza root passed through a 1000-mesh sieve was added to deionized water, wherein the weight ratio of the Salvia miltiorrhiza root to the deionized water was 1:20. After adjusting the pH to 4, a complex enzyme was added, wherein the weight ratio of the complex enzyme to the Salvia miltiorrhiza root was 1:3, and the complex enzyme included a mixture of cellulase, pectinase, and acid protease, wherein the ratio of cellulase: pectinase: acid protease was 2:1:0.3. After enzymatic hydrolysis, the enzymatic hydrolysis product was collected by centrifugation. The enzymatic hydrolysis temperature was 45° C., the enzymatic hydrolysis time was 30 min, and the centrifugal speed was 4000 r / min.

[0056] S2. Add 70% by volume ethanol solution to the enzymatic hydrolysate for ultrasonic extraction, filter, collect the filtrate, rotary evaporate at 40°C, and vacuum dry at 50°C for 48 hours to obtain the Salvia miltiorrhiza root extract; the weight ratio of the enzymatic hydrolysate to the ethanol solution is 1:30, the temperature of the ultrasonic extraction is 60°C, the time of the ultrasonic extraction is 20 minutes, and the ultrasonic power is 70w.

[0057] Salvia miltiorrhiza root extract B: homemade, the preparation method is different from that of Salvia miltiorrhiza root extract A only in that: in S1, the ratio of complex enzymes is different, cellulase: pectinase: acid protease = 1:2:0.5.

[0058] Salvia miltiorrhiza root extract C: homemade, the preparation method is different from that of Salvia miltiorrhiza root extract A only in that: in S1, the ratio of complex enzymes is different, cellulase: pectinase: acid protease = 0.5:3:0.5.

[0059] Salvia miltiorrhiza root extract D: homemade, the preparation method is different from that of Salvia miltiorrhiza root extract A only in that: in S1, the type and ratio of the complex enzyme are different, the complex enzyme includes a mixture of cellulase and pectinase, and the ratio of cellulase to pectinase is 2:1.

[0060] Salvia miltiorrhiza root extract E: homemade, the preparation method is different from that of Salvia miltiorrhiza root extract A only in that: in S2, the volume fraction of the ethanol solution is different, and a 50% volume fraction of ethanol solution is added to the enzymatic hydrolysate for ultrasonic extraction.

[0061] Salvia miltiorrhiza leaf extract A: Homemade, preparation method is as follows:

[0062] (1) drying and crushing the Salvia miltiorrhiza leaves to obtain Salvia miltiorrhiza leaf powder; the mesh size of the Salvia miltiorrhiza leaf powder is 50 mesh;

[0063] (2) Using 10 times the volume fraction of 70% ethanol solution to extract Salvia miltiorrhiza leaf powder, collecting the filtrate, rotary evaporating at 50°C and vacuum drying at 50°C for 48 hours to obtain the Salvia miltiorrhiza leaf extract; the extraction is ultrasonic extraction, the ultrasonic extraction temperature is 65°C, the ultrasonic extraction time is 1 hour, and the ultrasonic extraction power is 70W.

[0064] Salvia miltiorrhiza leaf extract B: homemade, the preparation method is different from that of Salvia miltiorrhiza leaf extract A only in that the volume fraction of the ethanol solution is different, and a volume fraction of 60% ethanol solution is used to extract Salvia miltiorrhiza leaf powder.

[0065] Salvia miltiorrhiza leaf extract C: homemade, the preparation method is different from that of Salvia miltiorrhiza leaf extract A only in that the volume fraction of the ethanol solution is different, and an 80% volume fraction ethanol solution is used to extract Salvia miltiorrhiza leaf powder.

[0066] Salvia miltiorrhiza leaf extract D: homemade, the preparation method is different from that of Salvia miltiorrhiza leaf extract A only in that the volume fraction of the ethanol solution is different, and a 50% volume fraction ethanol solution is used to extract Salvia miltiorrhiza leaf powder.

[0067] Table 1 shows the weight ratio of the root extract and the leaf extract in the Danshen extracts. Compared with Danshen extract 1, Danshen extracts 2 to 5 differ only in the type of the root extract, and Danshen extracts 6 to 8 differ only in the type of the leaf extract.

[0068] Table 1

[0069]

[0070]

[0071] Salvia miltiorrhiza extract 9

[0072] Compared with the Danshen Extract 1, the difference lies in that the weight ratio of the Danshen root extract A to the Danshen leaf extract A is different. Specifically, the weight ratio of the Danshen root extract to the Danshen leaf extract is 1:1.

[0073] Salvia miltiorrhiza extract 10

[0074] Compared with the Danshen Extract 1, the difference lies in the different weight ratio of the Danshen root extract A to the Danshen leaf extract A. Specifically, the weight ratio of the Danshen root extract to the Danshen leaf extract is 1:3.

[0075] Salvia miltiorrhiza extract 11

[0076] Compared with the Danshen Extract 1, the difference lies in the different weight ratio of the Danshen root extract A to the Danshen leaf extract A. Specifically, the weight ratio of the Danshen root extract to the Danshen leaf extract is 1:5.

[0077] Salvia miltiorrhiza extract 12

[0078] Compared with the Danshen Extract 1, the difference lies in the different weight ratio of the Danshen root extract A to the Danshen leaf extract A. Specifically, the weight ratio of the Danshen root extract to the Danshen leaf extract is 2:1.

[0079] Salvia miltiorrhiza extract 13

[0080] Compared with Danshen Extract 1, the difference is that it only contains Danshen root extract A.

[0081] Salvia miltiorrhiza extract 14

[0082] Compared with Danshen Extract 1, the difference is that it only contains Danshen Leaf Extract A.

[0083] Examples and Comparative Examples

[0084] The embodiments of the present invention and the comparative examples provide an anti-dandruff composition, the components and weight proportions of the composition are shown in Tables 2-3; wherein W represents the weight percentage of the Salvia miltiorrhiza extract based on the total weight of the anti-dandruff composition; wherein, Examples 1-12 differ only in the type of Salvia miltiorrhiza extract, and compared with Examples 1 and 13-16, the weight proportions of the components in the composition are different.

[0085] The preparation method of the composition comprises the following steps: uniformly mixing the components to obtain the anti-dandruff composition.

[0086] Table 2

[0087]

[0088] Example 2

[0089] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 2 is used in place of Salvia miltiorrhiza extract 1.

[0090] Example 3

[0091] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 3 is used in place of Salvia miltiorrhiza extract 1.

[0092] Example 4

[0093] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 4 is used in place of Salvia miltiorrhiza extract 1.

[0094] Example 5

[0095] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 5 is used in place of Salvia miltiorrhiza extract 1.

[0096] Example 6

[0097] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 6 is used in place of Salvia miltiorrhiza extract 1.

[0098] Example 7

[0099] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 7 is used in place of Salvia miltiorrhiza extract 1.

[0100] Example 8

[0101] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 8 is used in place of Salvia miltiorrhiza extract 1.

[0102] Example 9

[0103] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 9 is used in place of Salvia miltiorrhiza extract 1.

[0104] Example 10

[0105] The present example provides an anti-dandruff composition, which differs from Example 1 only in that Salvia miltiorrhiza extract 10 is used in place of Salvia miltiorrhiza extract 1.

[0106] Example 11

[0107] The embodiment of the present invention provides an anti-dandruff composition. Compared with Example 1, the anti-dandruff composition is different only in that: Salvia miltiorrhiza extract 11 is used to replace Salvia miltiorrhiza extract 1.

[0108] Example 12

[0109] The embodiment of the present invention provides an anti-dandruff composition. Compared with Example 1, the anti-dandruff composition is different only in that: Salvia miltiorrhiza extract 12 is used instead of Salvia miltiorrhiza extract 1.

[0110] Table 3

[0111]

[0112]

[0113] Application Examples

[0114] The application examples and comparative application examples of the present invention provide a shampoo, the components (mass percentage) of the shampoo are shown in Table 4; wherein, the anti-dandruff compositions used in Application Examples 1-16 are the anti-dandruff compositions prepared in Examples 1-16, for example, the anti-dandruff composition in Example 1 is used in Application Example 1, the anti-dandruff composition in Example 2 is used in Application Example 2, and so on; the anti-dandruff compositions used in Comparative Application Examples 1-12 are the anti-dandruff compositions prepared in Comparative Examples 1-12.

[0115] Table 4

[0116]

[0117] The preparation method of the shampoo provided in Application Example 1 comprises the following steps:

[0118] (1) Add polyquaternium-10 to deionized water and stir to disperse. After uniform dispersion, add other materials of phase A, heat to 85° C., stir to dissolve uniformly, and then keep warm for use to obtain prefabricated phase A;

[0119] (2) Add all ingredients of phase B at 85°C, stir thoroughly to dissolve evenly until transparent, and keep warm to remove foam;

[0120] (3) Cool to below 45°C, add the ingredients of Phase C one by one, and stir evenly;

[0121] (4) Continue to add the raw materials of phase D (previously dissolved in water), mix well, and obtain shampoo.

[0122] The preparation methods of the shampoos provided in the remaining application examples, comparative application examples, and blank application examples are consistent with that in Application Example 1, and no relevant components are added if they are not present.

[0123] Application Example 17

[0124] The application example of the present invention provides a shampoo, which is different from the shampoo of application example 1 in that the mass percentage of the anti-dandruff composition is 2%.

[0125] Blank Application Example

[0126] The blank application example of the present invention provides a shampoo, which differs from the application example 1 in that the anti-dandruff composition is not added.

[0127] Performance Test-1

[0128] The effects of the anti-dandruff compositions prepared in Examples 1-16 and Comparative Examples 1-12 were investigated, including the following aspects:

[0129] 1. Anti-inflammatory ability

[0130] Testing for anti-inflammatory capacity involves the following steps:

[0131] S1. Plating: RAW264.7 cells in the logarithmic growth phase were seeded in 24-well plates at a cell density of 2×10 5 / mL, 0.5mL per well, mark the top cover of the cell plate after laying the plate, and place it in a carbon dioxide incubator for incubation for 24h;

[0132] S2. Sample treatment: After incubating the cells for 24 h, the supernatant was removed and 0.5 mL of the following samples were added: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone (cell culture medium containing 0.001% dexamethasone) + LPS (1 μg / mL); ④ experimental group: composition (cell culture medium containing 0.5% of the composition of the embodiment or comparative example) + LPS (1 μg / mL);

[0133] S3. Detection of inflammatory factors in cell supernatant: After the cells were treated with the sample for 24 hours, the supernatant was aspirated into a centrifuge tube using a pipette and centrifuged at 1000 rpm for 5 minutes. The precipitate was discarded and the supernatant was aspirated. The changes in the amount of inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α Inflammatory Factor Detection Kit;

[0134] The inhibition rate of TNF-α was calculated as follows: TNF-α inhibition rate (%) = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group × 100%. A higher TNF-α inhibition rate (%) indicates a stronger anti-inflammatory ability.

[0135] The results are shown in Table 5.

[0136] 2. Inhibitory ability against 5α-reductase

[0137] The test for 5α-reductase inhibition involves the following steps:

[0138] S1. Sample tube: Add 1 mL of sample solution (2% aqueous solution of the composition of the embodiment or comparative example), 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution to a test tube, shake gently, and use a pipette to take 200 μL into a 96-well microplate. Make three replicates for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measurement value A. 样品0 After incubation at 37°C for 20 minutes, place in a microplate reader for detection and measure the absorbance at 340 nm, which is the second measurement value A. 样品20 ; For the positive control, 2.5 mmol / L finasteride was used to replace the sample solution;

[0139] S2, enzyme tube: add 1mL PBS solution, 1mL enzyme solution, 1mL NADPH solution and 1mL testosterone solution into the test tube, shake gently, take 200μL into 96-well microplate with a pipette, make three parallels for each sample, put it into the microplate reader for detection, measure the absorbance at 340nm, and use the first measurement value A 酶0 After the sample is incubated at 37°C for 20 minutes, it is placed in a microplate reader for detection and the absorbance value at 340 nm is measured as the second measurement value A. 酶20 The enzyme inhibition rate was calculated according to the following formula;

[0140]

[0141] Among them, the higher the 5α-reductase inhibition rate%, the stronger the oil control ability;

[0142] The results are shown in Table 5.

[0143] 3. Inhibitory effect on Malassezia

[0144] The test for the inhibitory ability against Malassezia includes the following steps:

[0145] The inhibition rate of the composition against Malassezia was tested using a turbidimetric method. Specifically, Malassezia (ATCC 44344) was cultured on a modified Dixon slant medium at 30°C for 72 hours. A loopful of the bacterial strain was streaked onto a modified Dixon agar plate, cultured at 30°C for 72 hours, and then inoculated into 50 mL of liquid modified Dixon agar. The suspension was shaken and cultured at 30°C and 130 rpm for 72 hours to obtain a bacterial suspension. The concentration of the suspension was determined by plate counting to be approximately 5×10 8 CFU / mL. Add 100 μL of YPD liquid medium and 50 μL of bacterial suspension to a 96-well plate and mix thoroughly. Then, add 50 μL of a 1% aqueous solution of the composition. A plate containing only the bacterial suspension serves as a growth control, while a plate containing only the medium serves as a sterile control. Incubate the 96-well plate at 30°C for 48 hours, then measure absorbance at 600 nm. Calculate the inhibition rate according to the following formula.

[0146]

[0147] The results are shown in Table 5.

[0148] Table 5

[0149]

[0150]

[0151] From the above table, the anti-dandruff composition prepared by the embodiment of the application has a TNF-α inhibition rate of 20% or more, a 5α-reductase inhibition rate of 30% or more, and a Malassezia inhibition rate of 60% or more. The Norway spruce leaf extract, the Hypericum perforatum extract, the Salvia miltiorrhiza extract, and the Olea europaea leaf extract are synergistic with each other, and the prepared composition has a good effect of controlling oil and reducing dandruff.

[0152] From the comparison of Examples 1-12, it can be seen that the preparation method of the Salvia miltiorrhiza root extract and the Salvia miltiorrhiza leaf extract, and the ratio of the Salvia miltiorrhiza root extract and the Salvia miltiorrhiza leaf extract, will affect the effect of controlling oil and reducing dandruff of the final obtained anti-dandruff composition. The effect of controlling oil and reducing dandruff of the final obtained anti-dandruff composition is better when the specific preparation method and the ratio are used.

[0153] From the comparison of Example 1 and Examples 13-16, it can be seen that the weight parts of the components in the composition will affect the performance of the product. When the weight parts of the components are further selected within the preferred range, the effect of controlling oil and reducing dandruff of the obtained anti-dandruff composition is better.

[0154] From the comparison of Example 1 and Comparative Examples 1-12, it can be seen that when one or two of the components are not added, or when the components are not added while the partitions of other components are supplemented, the effect of the application cannot be achieved. When other similar components are used to replace, and when the weight parts of the components in the composition are not within the range of the application, the synergistic effect cannot be well played.

[0155] Performance test-2

[0156] The application effect of the shampoo prepared by the effect example, the application examples 1-17, the comparative application examples 1-12, and the blank application example on human body is explored, including the following steps: selecting a plurality of male volunteers aged 18-45 years old, who have moderate dandruff and moderate oily scalp conditions. The subjects sit in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. The initial oil content is measured by using a scalp oil test instrument Meibometer MB560, and the test site is the top of the head. The skin transdermal water loss probe The TM Hex test uses an initial TEWL value at the hairline. Professionals comb the hair 100 times with a standard non-static comb to collect dandruff. The collected dandruff is placed on a black board, photographed, and analyzed using Image Pro Plus 7.0.

[0157] The subjects were randomly divided into 30 groups, with 5 people in each group. The shampoo provided by the present invention was used once a day. The oil content, dandruff count, and TEWL value were tested at 4 weeks and 8 weeks. The improvement of each indicator of the volunteers at 4 and 8 weeks was analyzed, and the improvement was represented by the change rate of each indicator.

[0158] The calculation is as follows:

[0159] The change rate % relative to the initial value = |(X after use - X before use) / X before use × 100%|; wherein, the greater the change rate % relative to the initial value, the stronger the improvement effect.

[0160] Table 6

[0161]

[0162]

[0163] As can be seen from the above table, the anti-dandruff composition prepared in the embodiment of the present invention has good effects of controlling oil, reducing dandruff, and repairing scalp homeostasis when used to prepare shampoo.

[0164] From the comparison of Application Examples 1-12, it can be seen that when the prepared anti-dandruff composition is used to prepare shampoo, the preparation method of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract in the composition, as well as the ratio of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract, will affect the effect of the final anti-dandruff composition in controlling oil and reducing dandruff. By adopting the specific preparation method and ratio of the present invention, the final anti-dandruff composition has a better effect in controlling oil and reducing dandruff.

[0165] From the comparison of Application Example 1 and Application Examples 13-16, it can be seen that when the prepared anti-dandruff composition is used to prepare shampoo, the weight of the components in the composition will affect the performance of the product. When the weight of the components is further selected within the preferred range, the obtained anti-dandruff composition has a better effect in controlling oil and reducing dandruff.

[0166] From the comparison of Application Example 1 and Application Examples 1-12, it can be seen that when the anti-dandruff composition is used to prepare shampoo, when one or two of the components are not added, or when the other components are used to make up the difference without adding them, the effect of the present invention cannot be achieved; and when other similar ingredients are used as substitutes, when the weight proportions of the components in the composition are not within the scope of this application, a good synergistic effect cannot be achieved.

[0167] Performance Test 3

[0168] The present invention verifies the effects of the product prepared in Application Example 1 on oil control and dandruff removal for male consumers with sensitive skin, and specifically comprises the following steps:

[0169] Thirty male subjects with sensitive skin were selected for the test. This product was distributed to them and they used the shampoo prepared in Application Example 1 daily for an eight-week period. The subjects completed questionnaires on the degree of improvement in indicators such as oil control and anti-dandruff effects. After stopping use of the product, they switched to using the shampoo prepared in the blank application example and completed questionnaires on anti-dandruff effects again after two and four weeks. See Table 7 below for details.

[0170] Table 7

[0171]

[0172]

[0173] As can be seen from the above table, the product prepared using the composition of the present invention can better maintain the balance of microecology, and the dandruff problem will not recur after the product is discontinued.

[0174] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-dandruff composition, characterized in that The invention comprises the following components in parts by weight: 0.5-5 parts of Norway spruce leaf extract, 0.1-2 parts of Hypericum perforatum extract, 0.1-2 parts of Salvia miltiorrhiza extract, and 0.5-5 parts of olive leaf extract; The salvia miltiorrhiza extract is a mixture of salvia miltiorrhiza root extract and salvia miltiorrhiza leaf extract.

2. The anti-dandruff composition according to claim 1, wherein The invention comprises the following components in parts by weight: 1-3 parts of Norway spruce leaf extract, 0.5-1 parts of Hypericum perforatum extract, 0.5-1 parts of Salvia miltiorrhiza extract and 1-2 parts of olive leaf extract.

3. The anti-dandruff composition according to claim 1, wherein Based on the total weight of the anti-dandruff composition, the weight percentage of the salvia miltiorrhiza extract is 15-25%.

4. The anti-dandruff composition according to claim 1, wherein The weight ratio of the salvia miltiorrhiza root extract to the salvia miltiorrhiza leaf extract is 1:(1-3).

5. The anti-dandruff composition according to claim 1, wherein The preparation method of the salvia miltiorrhiza root extract comprises the following steps: S1, taking dried, crushed and sieved Salvia miltiorrhiza root, adding it to deionized water, adjusting the pH to 3-4, adding a complex enzyme, wherein the complex enzyme includes a mixture of cellulase, pectinase and acid protease, and collecting the enzymatic hydrolysis product after enzymolysis; S2. Adding an organic solvent to the enzymatic hydrolyzate for ultrasonic extraction, filtering, collecting the filtrate, and rotary evaporating the filtrate and drying it to obtain the Salvia miltiorrhiza root extract.

6. The anti-dandruff composition according to claim 5, characterized in that The weight ratio of the complex enzyme to the root of Salvia miltiorrhiza is 1:(2-4), and the weight ratio of cellulase, pectinase and acid protease in the complex enzyme is cellulase:pectinase:acid protease=(1-3):(0.5-2):(0.1-0.5); And / or, the organic solvent is an ethanol solution with a volume fraction of 60-70%, and the weight ratio of the enzymatic hydrolysate to the ethanol solution is 1:(20-40); and / or, the mesh size of the sieve is 800-1000 mesh, and the weight ratio of the salvia miltiorrhiza root to deionized water is 1:(10-20); And / or, the enzymatic hydrolysis temperature is 40-45° C., the enzymatic hydrolysis time is 20-30 min; after the enzymatic hydrolysis, the enzymatic hydrolysis product is collected by centrifugation at a speed of 3000-5000 r / min; And / or, the temperature of the ultrasonic extraction is 50-60°C, the time of the ultrasonic extraction is 15-30 minutes, and the ultrasonic power is 60-75w; And / or, the rotary evaporation temperature is 40-50°C; And / or, the drying condition is vacuum drying at 50-60° C. for 12-48 hours; And / or, the enzymatic activity of the cellulase is 20-50 U / mg, the enzymatic activity of the pectinase is 20-50 U / mg, and the enzymatic activity of the acid protease is 30-80 U / mg.

7. The anti-dandruff composition according to claim 1, wherein The preparation method of the Salvia miltiorrhiza leaf extract comprises the following steps: (1) drying and crushing the Salvia miltiorrhiza leaves to obtain Salvia miltiorrhiza leaf powder; the mesh size of the Salvia miltiorrhiza leaf powder is 40-50 mesh; (2) Extracting the Danshen leaf powder with 8-10 times the amount of ethanol solution, collecting the filtrate, rotary evaporating and drying to obtain the Danshen leaf extract.

8. The anti-dandruff composition according to claim 7, wherein The volume fraction of the ethanol solution is 60-70%; And / or, the extraction is ultrasonic extraction, the ultrasonic extraction temperature is 55-65° C., the ultrasonic extraction time is 1-2 h, and the ultrasonic extraction power is 60-70 W; And / or, the rotary evaporation temperature is 40-50°C.

9. Use of the anti-dandruff composition according to any one of claims 1 to 8 in the preparation of toiletries.

10. A toiletries product, characterized in that: The toiletries include the anti-dandruff composition according to any one of claims 1 to 8.

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