Pharmaceutical composition for treating endometritis as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a pharmaceutical composition for treating endometritis and a preparation method and application thereof. The pharmaceutical composition for treating endometritis, provided by the invention, is prepared from the following raw materials in parts by weight: 10 to 30 parts of radix salviae miltiorrhizae, 8 to 25 parts of rhizoma curcumae, 8 to 25 parts of rhizoma sparganii, 5 to 15 parts of radix gentianae, 10 to 30 parts of herba taraxaci, 10 to 25 parts of fructus forsythiae, 8 to 20 parts of rhizoma chuanxiong, 5 to 15 parts of fructus cnidii, 8 to 20 parts of rhizoma cyperi, 15 to 35 parts of radix astragali seu hedysari and 3 to 10 parts of liquorice root. The medicine composition has the main effects of promoting blood circulation to remove blood stasis and clearing away heat and toxic materials, has the auxiliary effects of regulating qi, eliminating dampness, warming yang and the like, and achieves the purpose of treating endometritis by improving blood circulation of endometrium, eliminating inflammation and regulating endocrine through the synergistic effect of multiple aspects.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a pharmaceutical composition for treating endometritis, its preparation method and uses. Background Art

[0002] Endometritis is a common inflammatory disease in gynecology, mainly occurring in the endometrial tissue. According to the length of the course of the disease, it is divided into acute endometritis and chronic endometritis. After the occurrence of endometritis, the entire uterine cavity often undergoes edema and exudation. In the acute phase, it can also lead to systemic symptoms such as fever, chills, increased white blood cells, lower abdominal pain, increased leucorrhea, sometimes bloody or foul-smelling, and sometimes the uterus is slightly enlarged and tender. The manifestations of the chronic form are basically the same, and there may also be excessive menstruation, lower abdominal pain and obvious lumbosacral distension. Endometritis generally belongs to the categories of "leucorrhea disease", "abdominal pain" or "abdominal mass" in traditional Chinese medicine, and is mostly related to damp-heat stasis and poor qi and blood circulation.

[0003] Although the incidence of endometritis is relatively low, once a person gets the disease, it will bring many impacts on life and work, and may even further develop into myometritis, salpingitis and pelvic inflammatory disease, aggravating the condition and bringing pain to the patient, so its harm cannot be underestimated.

[0004] Currently, the western medicine for treating endometritis is antibiotics, but long-term use of antibiotics will produce side effects and drug resistance, and cannot cure the disease. Therefore, researching a traditional Chinese medicine composition for treating endometritis has important clinical significance. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a pharmaceutical composition for treating endometritis.

[0006] The pharmaceutical composition provided by the present invention comprises raw materials in the following weight parts: 10 - 30 weight parts of Salvia miltiorrhiza, 8 - 25 weight parts of Rhizoma zedoariae, 8 - 25 weight parts of Rhizoma sparganii, 5 - 15 weight parts of Gentiana scabra, 10 - 30 weight parts of Taraxacum mongolicum, 10 - 25 weight parts of Forsythia suspensa, 8 - 20 weight parts of Ligusticum chuanxiong, 5 - 15 weight parts of Fructus cnidii, 8 - 20 weight parts of Cyperus rotundus, 15 - 35 weight parts of Astragalus membranaceus, 3 - 10 weight parts of Glycyrrhiza uralensis.

[0007] Further, the pharmaceutical composition comprises raw materials in the following weight parts: 10 - 20 weight parts of Salvia miltiorrhiza, 10 - 15 weight parts of Rhizoma zedoariae, 10 - 15 weight parts of Rhizoma sparganii, 5 - 10 weight parts of Gentiana scabra, 10 - 20 weight parts of Taraxacum mongolicum, 10 - 15 weight parts of Forsythia suspensa, 10 - 15 weight parts of Ligusticum chuanxiong, 5 - 10 weight parts of Fructus cnidii, 10 - 15 weight parts of Cyperus rotundus, 15 - 25 weight parts of Astragalus membranaceus, 5 - 10 weight parts of Glycyrrhiza uralensis.

[0008] Preferably, the pharmaceutical composition comprises the following raw materials in parts by weight: 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Rhizoma Curcumae, 10 parts by weight of Sparganii Rhizoma, 8 parts by weight of Gentianae Radix, 18 parts by weight of Taraxaci Herba, 15 parts by weight of Forsythiae Fructus, 15 parts by weight of Chuanxiong Rhizoma, 8 parts by weight of Cnidii Fructus, 12 parts by weight of Cyperi Rhizoma, 25 parts by weight of Astragali Radix, and 6 parts by weight of Glycyrrhizae Radix.

[0009] Salvia miltiorrhiza is the dried root and rhizome of the plant Salvia miltiorrhiza Bge. of the Labiatae family. It is bitter and slightly cold in nature. It belongs to the heart and liver meridians. It promotes blood circulation to remove stasis, dredges meridians to relieve pain, clears the heart to remove vexation, and cools blood to eliminate carbuncles. It is used for chest pain due to chest impediment, pain in the epigastrium, flanks and abdomen, mass accumulation, arthralgia due to heat, restlessness and insomnia, irregular menstruation, dysmenorrhea, amenorrhea, and swelling and pain of sores and ulcers.

[0010] Rhizoma Curcumae is the dried rhizome of the plants Curcuma phaeocaulis Val. of the Zingiberaceae family, Curcuma kwangsiensis S.G.Lee et C.F.Liang, or Curcuma wenyujin Y.H.Chen et C.Ling. It is pungent, bitter, and warm in nature. It belongs to the liver and spleen meridians. It promotes qi circulation to break blood stasis and eliminates accumulation to relieve pain. It is used for mass accumulation, static blood amenorrhea, chest pain due to chest impediment, and abdominal distension and pain due to food accumulation.

[0011] Sparganii Rhizoma is the dried tuber of the plant Sparganium stoloniferum Buch.-Ham. of the Sparganiaceae family. It is pungent, bitter, and neutral in nature. It belongs to the liver and spleen meridians. It breaks blood and promotes qi circulation, eliminates accumulation to relieve pain. It is used for mass accumulation, dysmenorrhea, static blood amenorrhea, chest pain due to chest impediment, and abdominal distension and pain due to food accumulation.

[0012] Gentianae Radix is the dried root and rhizome of the plants Gentiana manshurica Kitag., Gentiana scabra Bge., Gentiana triflora Pall., or Gentiana rigescens Franch. of the Gentianaceae family. The first three are commonly known as "Gentianae Radix", and the latter is commonly known as "Firm Gentianae Radix". It is bitter and cold in nature. It belongs to the liver and gallbladder meridians. It clears heat and dries dampness, purges the liver and gallbladder fire. It is used for damp-heat jaundice, swelling and itching of the vulva, leukorrhea, eczema and itching, redness of the eyes due to liver fire, tinnitus and deafness, pain in the flanks and bitter taste in the mouth, priapism, and infantile convulsions.

[0013] Taraxaci Herba is the dried whole herb of the plants Taraxacum mongolicum Hand.-Mazz., Taraxacum borealisinense Kitam., or several plants of the same genus of the Compositae family. It is bitter, sweet, and cold in nature. It belongs to the liver and stomach meridians. It clears heat and detoxifies, reduces swelling and dissipates nodules, promotes diuresis and relieves stranguria. It is used for furuncles and carbuncles, breast abscess, scrofula, redness of the eyes, sore throat, lung abscess, intestinal abscess, damp-heat jaundice, and stranguria with pain.

[0014] Forsythia fruit is the dried fruit of Forsythia suspensa (Thunb.) Vahl, a plant of the Oleaceae family. It is bitter in taste and slightly cold in nature. It acts on the lung, heart, and small intestine meridians. It clears heat and detoxifies, reduces swelling and dissipates nodules, and disperses wind-heat. It is used for carbuncles, scrofula, breast abscess, erysipelas, wind-heat cold, the initial stage of warm diseases, invasion of the nutrient aspect by warmth, high fever with restlessness and thirst, coma with maculae, and painful strangury.

[0015] Chuanxiong rhizome is the dried rhizome of Ligusticum chuanxiong Hort., a plant of the Umbelliferae family. It is pungent in taste and warm in nature. It acts on the liver, gallbladder, and pericardium meridians. It promotes blood circulation and qi movement, dispels wind and relieves pain. It is used for chest impediment pain, stabbing pain in the chest and hypochondrium, swelling and pain from falls and bruises, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to masses, headache, and rheumatic arthralgia.

[0016] Cnidium fruit is the dried ripe fruit of Cnidium monnieri (L.) Cuss., a plant of the Umbelliferae family. It is pungent, bitter, and warm in nature; slightly toxic. It acts on the kidney meridian. It dries dampness, dispels wind, kills parasites and relieves itching, warms the kidney and invigorates yang. It is used for itching in the vulva and leukorrhea, eczema with itching, painful obstruction of the lower back due to dampness, impotence due to kidney deficiency, and infertility due to cold uterus.

[0017] Cyperus rhizome is the dried rhizome of Cyperus rotundus L., a plant of the Cyperaceae family. It is pungent, slightly bitter, slightly sweet, and neutral in nature. It acts on the liver, spleen, and triple energizer meridians. It soothes the liver and relieves depression, regulates qi and alleviates fullness, regulates menstruation and relieves pain. It is used for stagnation of liver qi, distending pain in the chest and hypochondrium, pain in the hernia, distending pain in the breasts, stagnation of qi in the spleen and stomach, stuffiness and fullness in the epigastrium and abdomen, distending pain, irregular menstruation, amenorrhea and dysmenorrhea.

[0018] Astragalus root is the dried root of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge., plants of the Leguminosae family. It is sweet in taste and slightly warm in nature. It acts on the lung and spleen meridians. It supplements qi and raises yang, strengthens the superficial resistance to stop sweating, promotes diuresis to reduce edema, generates body fluid and nourishes blood, promotes qi movement to relieve pain, supports the healthy qi to expel pus, and astringes sore to promote granulation. It is used for qi deficiency with weakness, poor appetite and loose stools, sinking of the middle qi, chronic diarrhea with rectal prolapse, hematochezia and metrorrhagia, spontaneous sweating due to deficiency of the superficial resistance, qi deficiency edema, internal heat with thirst, sallow complexion due to blood deficiency, hemiplegia, arthralgia and numbness, difficulty in ulceration of carbuncles and sores, and non-healing of chronic ulcers.

[0019] Licorice is the dried root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of the Leguminosae family. It is sweet in taste and neutral in nature. It acts on the heart, lung, spleen and stomach meridians. It invigorates the spleen and replenishes qi, clears heat and detoxifies, reduces phlegm and relieves cough, alleviates spasm and pain, and harmonizes various medicinal herbs. It is used for weakness of the spleen and stomach, lassitude and weakness, palpitations and shortness of breath, cough with copious phlegm, spasmodic pain in the abdomen and limbs, carbuncles and sores, and to relieve the toxicity and potency of medicinal herbs.

[0020] Endometritis belongs to the categories of "leucorrhea disease", "abdominal pain" or "abdominal mass", etc., and is mostly related to damp-heat stasis and poor qi and blood circulation. In the composition of the present invention, Salvia miltiorrhiza promotes blood circulation to remove blood stasis, dredges the channels and relieves pain, clears the heart and removes vexation, cools the blood and eliminates carbuncles. The tanshinone compounds contained therein have anti-inflammatory and antioxidant effects, can improve the local blood circulation of the endometrium, and reduce the inflammatory reaction; Rhizoma zedoariae promotes qi and breaks blood stasis, eliminates accumulation and relieves pain, and zedoary oil has anti-tumor and anti-inflammatory effects, which helps to eliminate endometrial inflammation and tissue repair; Rhizoma sparganii breaks blood and promotes qi, eliminates accumulation and relieves pain. The three together serve as the monarch drugs, targeting the core pathogenesis of endometritis, blood stasis blockage, to promote blood circulation to remove blood stasis and dredge the channels, improve the blood circulation of the endometrium, and reduce the pain and menstrual abnormalities caused by blood stasis blockage.

[0021] Gentiana scabra clears heat and dries dampness, purges the liver and gallbladder fire, and its active ingredients can inhibit the growth and reproduction of bacteria; Taraxacum mongolicum Hand.-Mazz. clears heat and detoxifies, reduces swelling and dissipates nodules, induces diuresis and relieves stranguria, and has good treatment for inflammation caused by damp-heat accumulation; Forsythia suspensa (Thunb.) Vahl clears heat and detoxifies, reduces swelling and dissipates nodules, disperses wind-heat, and helps to remove endometrial inflammation. Endometritis is mostly accompanied by damp-heat accumulation. The three together serve as the ministerial drugs, can clear heat and promote diuresis, detoxify and reduce inflammation, relieve the damp-heat syndrome caused by inflammation, and help to improve the inflammatory environment of the endometrium in the formula.

[0022] Chuanxiong rhizome promotes blood circulation and qi movement, dispels wind and relieves pain; Fructus cnidii dries dampness and expels wind, kills insects and relieves itching, warms the kidney and invigorates yang, and can be used to improve symptoms such as abnormal leucorrhea caused by damp-heat pouring downward, and assist in clearing heat and drying dampness; Rhizoma cyperi soothes the liver and relieves depression, regulates qi and soothes the middle energizer, regulates menstruation and relieves pain; Astragalus membranaceus (Fisch.) Bunge replenishes qi and raises yang, consolidates the exterior and stops sweating. While promoting blood circulation to remove blood stasis, it supplements vital qi, enhances the disease resistance of the body, and prevents damage to vital qi. The three together serve as the adjuvant drugs.

[0023] Licorice harmonizes various medicinal herbs, moderates the properties of medicinal herbs, plays a role in harmonizing each medicinal herb in the formula, reduces the interaction between medicinal herbs, and at the same time moderates the properties of medicinal herbs, protects the spleen and stomach, and serves as the guiding drug.

[0024] The composition of the present invention mainly focuses on promoting blood circulation to remove blood stasis and dredging the channels, supplemented by clearing heat and detoxifying, replenishing qi and consolidating the exterior, warming yang and drying dampness, etc. to achieve a synergistic effect and achieve the purpose of treating endometritis.

[0025] The present invention also provides a method for preparing the pharmaceutical composition for treating endometritis, comprising the following steps: washing salvia miltiorrhiza, zedoaria, trifoliate green, gentian, dandelion, forsythia, ligusticum chuanxiong, cnidium monnieri, cyperus rotundus, astragalus and liquorice, crushing after drying, mixing the crushed medicinal materials evenly, soaking them in water, decocting them for 2 to 3 times, filtering, combining the decoctions, concentrating them into extracts, and drying them to obtain extract powder.

[0026] Furthermore, in the method for preparing the pharmaceutical composition for treating endometritis,

[0027] The amount of water added is 8 to 10 times the total amount of the original medicinal materials.

[0028] The soaking time is 30 to 60 minutes.

[0029] The decocting time is 1 to 2 hours.

[0030] The combined decoctions are concentrated to an extract having a relative density of 1.10 to 1.20 at 60°C.

[0031] The drying is spray drying or vacuum drying.

[0032] The present invention also provides application of the pharmaceutical composition in preparing a medicine for treating endometritis.

[0033] Furthermore, the drug for treating endometritis is composed of the pharmaceutical composition and a pharmaceutically acceptable carrier.

[0034] Furthermore, the endometritis includes acute endometritis and chronic endometritis.

[0035] The beneficial effects of the present invention are:

[0036] The present invention uses salvia miltiorrhiza, zedoaria, trifoliate lancea, gentian, dandelion, forsythia, chuanxiong, cnidium monnieri, cyperus rotundus, astragalus and liquorice to prepare a prescription. Salvia miltiorrhiza, zedoaria and trifoliate lancea are used as the main medicines, and the core pathogenesis of endometritis, blood stasis, blockage, plays the role of promoting blood circulation and removing blood stasis, forcing blood stasis and unblocking collaterals, improves the blood circulation of the endometrium, and reduces the pain and menstrual abnormalities caused by blood stasis. Gentiana, dandelion and forsythia are used as the ministerial medicines, clearing away heat and dampness, detoxifying and anti-inflammatory, relieving the damp-heat syndrome caused by inflammation, and helping to improve the inflammatory environment of the endometrium in the prescription. Chuanxiong, cnidium monnieri, cyperus rotundus and astragalus are used as adjuvants, while promoting blood circulation and removing blood stasis, replenishing healthy energy, enhancing the body's disease resistance, and preventing the healthy energy from being damaged. Licorice harmonizes various drugs, relaxes the properties of the drugs, plays the role of harmonizing various drugs in the prescription, reduces the interaction between drugs, relaxes the properties of the drugs, protects the spleen and stomach, and is the guiding drug.

[0037] The pharmaceutical composition of the present invention mainly promotes blood circulation to remove blood stasis and clears heat and detoxifies, supplemented by functions such as regulating qi, drying dampness, and warming yang. Through the synergistic effects in multiple aspects, it improves the blood circulation of the endometrium, clears inflammation, regulates endocrine, and achieves the purpose of treating endometritis. Experiments have proved that the traditional Chinese medicine composition of the present invention can increase the content of immunoglobulins, strengthen the body's immune protection, and improve the local lesions of endometritis. Detailed implementation manners

[0038] The present invention will be further elaborated in detail through specific embodiments below. The raw materials and reagents involved in the detailed implementation manners are all obtained through commercial channels.

[0039] Example 1

[0040] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, 15 parts by weight of Forsythia suspensa, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, 25 parts by weight of Astragalus membranaceus, and 6 parts by weight of Glycyrrhiza uralensis. Wash, dry and then crush them. Mix the crushed medicinal materials evenly, add 8 times the total amount of the original medicinal materials of water and soak for 45 minutes, decoct twice, the first decoction for 2 hours, the second decoction for 1.5 hours, filter, combine the decoction liquids, concentrate to an extract with a relative density of 1.10 - 1.20 at 60 °C, and obtain the extract powder by spray drying.

[0041] Example 2

[0042] Weigh 10 parts by weight of Salvia miltiorrhiza, 8 parts by weight of Curcuma zedoaria, 8 parts by weight of Sparganium stoloniferum, 5 parts by weight of Gentiana scabra, 10 parts by weight of Taraxacum mongolicum, 10 parts by weight of Forsythia suspensa, 8 parts by weight of Ligusticum chuanxiong, 5 parts by weight of Cnidium monnieri, 8 parts by weight of Cyperus rotundus, 15 parts by weight of Astragalus membranaceus, and 3 parts by weight of Glycyrrhiza uralensis. Wash, dry and then crush them. Mix the crushed medicinal materials evenly, add 9 times the total amount of the original medicinal materials of water and soak for 30 minutes, decoct twice, the first decoction for 2 hours, the second decoction for 1 hour, filter, combine the decoction liquids, concentrate to an extract with a relative density of 1.10 - 1.20 at 60 °C, and obtain the extract powder by vacuum drying.

[0043] Example 3

[0044] Weigh 30 parts by weight of Salvia miltiorrhiza, 25 parts by weight of Curcuma zedoaria, 25 parts by weight of Sparganium stoloniferum, 15 parts by weight of Gentiana scabra, 30 parts by weight of Taraxacum mongolicum, 25 parts by weight of Forsythia suspensa, 20 parts by weight of Ligusticum chuanxiong, 15 parts by weight of Cnidium monnieri, 20 parts by weight of Cyperus rotundus, 35 parts by weight of Astragalus membranaceus, and 10 parts by weight of Glycyrrhiza uralensis. Wash, dry and then crush them. Mix the crushed medicinal materials evenly, add 10 times the total amount of the original medicinal materials of water and soak for 60 minutes, decoct twice, the first decoction for 2 hours, the second decoction for 2 hours, filter, combine the decoction liquids, concentrate to an extract with a relative density of 1.10 - 1.20 at 60 °C, and obtain the extract powder by spray drying.

[0045] Example 4

[0046] Weigh 20 parts by weight of Salvia miltiorrhiza, 15 parts by weight of Curcuma zedoaria, 15 parts by weight of Sparganium stoloniferum, 10 parts by weight of Gentiana scabra, 20 parts by weight of Taraxacum mongolicum, 18 parts by weight of Forsythia suspensa, 14 parts by weight of Ligusticum chuanxiong, 10 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, 25 parts by weight of Astragalus membranaceus, and 7 parts by weight of Glycyrrhiza uralensis. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 10 times the total amount of the original medicinal materials in water for 60 minutes, decoct them twice, decoct for 2 hours for the first time and 2 hours for the second time, filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0047] Example 5 Tablets

[0048] Prepare the extract powder obtained in Example 1 into tablets by referring to the conventional traditional Chinese medicine preparation method.

[0049] Example 6 Granules

[0050] Prepare the extract powder obtained in Example 1 into granules by referring to the conventional traditional Chinese medicine preparation method.

[0051] Example 7 Capsules

[0052] Prepare the extract powder obtained in Example 1 into capsules by referring to the conventional traditional Chinese medicine preparation method.

[0053] Comparative Example 1

[0054] Weigh 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, 15 parts by weight of Forsythia suspensa, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, 25 parts by weight of Astragalus membranaceus, and 6 parts by weight of Glycyrrhiza uralensis. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials in water for 45 minutes, decoct them twice, decoct for 2 hours for the first time and 1.5 hours for the second time, filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0055] Comparative Example 2

[0056] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, 25 parts by weight of Astragalus membranaceus, and 6 parts by weight of Glycyrrhiza uralensis. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials in water for 45 minutes, decoct them twice, decoct for 2 hours for the first time and 1.5 hours for the second time, filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0057] Comparative Example 3

[0058] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, and 6 parts by weight of Glycyrrhiza uralensis. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials of water for 45 minutes, decoct them twice. The first decoction is for 2 hours, and the second decoction is for 1.5 hours. Filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0059] Comparative Example 4

[0060] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, 15 parts by weight of Forsythia suspensa, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, and 25 parts by weight of Astragalus membranaceus. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials of water for 45 minutes, decoct them twice. The first decoction is for 2 hours, and the second decoction is for 1.5 hours. Filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0061] Comparative Example 5

[0062] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, and 15 parts by weight of Forsythia suspensa. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials of water for 45 minutes, decoct them twice. The first decoction is for 2 hours, and the second decoction is for 1.5 hours. Filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0063] Comparative Example 6

[0064] Weigh 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Curcuma zedoaria, 10 parts by weight of Sparganium stoloniferum, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, and 25 parts by weight of Astragalus membranaceus. Wash them, dry them, and then crush them. Mix the crushed medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials of water for 45 minutes, decoct them twice. The first decoction is for 2 hours, and the second decoction is for 1.5 hours. Filter, combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60°C, and spray-dry to obtain an extract powder.

[0065] Comparative Example 7

[0066] Weigh 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, 15 parts by weight of Forsythia suspensa, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Cnidium monnieri, 12 parts by weight of Cyperus rotundus, and 25 parts by weight of Astragalus membranaceus. Wash them, dry them, and then pulverize them. Mix the pulverized medicinal materials evenly, soak them in 8 times the total amount of the original medicinal materials in water for 45 minutes, decoct them twice. The first decoction is for 2 hours, and the second decoction is for 1.5 hours. Filter and combine the decoction liquids, concentrate them to an extract with a relative density of 1.10 - 1.20 at 60 °C, and obtain the extract powder by spray drying.

[0067] Effect of the experimental composition on experimental endometritis in rats

[0068] Experimental animals: 130 female SD rats, 10 - 12 weeks old, weighing 210 - 250 g, with normal estrous cycles, purchased from Henan Experimental Animal Center, license number: SCXK(Yu)2019 - 0315.

[0069] Animal modeling: After anesthetizing SD female rats by intraperitoneal injection of 30 mg / kg sodium pentobarbital, disinfect the lower abdomen routinely and make a median incision. Expose the uterus, use a 0.25 mL syringe equipped with a No. 4 needle, insert the needle into the uterine cavity at the uterine bifurcation, and slowly inject 0.05 mL of 25% phenol glue slurry per rat towards the ovarian direction, and then suture layer by layer to close the abdomen, serving as the modeled animals. Additionally, leave 10 rats with the same surgical operation as above, but without giving phenol glue slurry, serving as the sham operation control group. After the operation, no treatment is given to the animals, and they are allowed to freely eat and drink water.

[0070] On the 8th day after modeling, randomly divide the modeled animals into 12 groups, with 10 rats in each group: model control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, Comparative Example 6 group, Comparative Example 7 group. Administer drugs by gavage according to the dose grouping. The model control group and the sham operation group are given the same volume of distilled water, and the Example groups and the Comparative Example groups are respectively given the extract powders prepared in Examples 1 - 4 and Comparative Examples 1 - 7 of the present invention. The administration dose is calculated as 2.93 g / kg of the crude drug amount.

[0071] 24 hours after the last drug administration, anesthetize the animals, draw blood from the abdominal aorta, and measure the contents of immunoglobulin IgG, IgA, and IgM by the single radial immunodiffusion method. The experimental data are statistically processed using the t - test. Additionally, surgically remove the bilateral uterus and fix it in 10% formaldehyde solution. Embed it in paraffin routinely and section it. Stain with HE. Divide the uterine tissue into four layers: lumen, mucosa, muscular layer, and serosa. Read the slides layer by layer under a light microscope. Observe the degree of uterine cavity adhesion, occlusion or dilation, and the change of the cavity wall structure; the integrity of the mucosal epithelium; the type, quantity, and infiltration degree of chronic inflammatory cells in the muscular layer and serosa layer; the degree of fibroblast proliferation, and the presence or absence of congestion, edema, and degeneration necrosis.

[0072] The content of serum immunoglobulin in the rats of the model control group was significantly lower than that of the sham operation control group, and the difference between the two groups was very significant (P<0.05 or P<0.01). The content of serum immunoglobulin in the animals of the example group was significantly increased, and the difference was significant compared with the model control group (P<0.05 or P<0.01). The specific results are shown in Table 1.

[0073] Table 1 Comparison of blood immunoglobulin content in rats of each group (g / L)

[0074] Group IgG IgA IgM Model control group <![CDATA[0.305±0.34 * > <![CDATA[0.051±0.28 ** > <![CDATA[0.52±0.52 ** > Sham operation group 0.379±0.41 0.082±0.23 0.78±0.46 Example 1 group <![CDATA[0.364±0.40 # > <![CDATA[0.073±0.36 # > <![CDATA[0.74±0.39 ## > Example 2 group <![CDATA[0.343±0.32 # > <![CDATA[0.060±0.26 # > <![CDATA[0.68±0.42 # > Example 3 group <![CDATA[0.358±0.35 # > <![CDATA[0.067±0.31 # > <![CDATA[0.72±0.45 # > Example 4 group <![CDATA[0.352±0.37 # > <![CDATA[0.063±0.29 # > <![CDATA[0.70±0.38 # > Comparative example 1 group 0.331±0.51 0.056±0.42 0.57±0.29 Comparative example 2 group 0.335±0.38 0.057±0.53 0.58±0.22 Comparative example 3 group 0.338±0.27 0.057±0.47 0.60±0.37 Comparative example 4 group 0.341±0.36 0.058±0.31 0.62±0.42 Comparative example 5 group 0.329±0.35 0.055±0.58 0.55±0.51 Comparative example 6 group 0.326±0.49 0.054±0.45 0.54±0.44 Comparative example 7 group 0.324±0.43 0.052±0.36 0.54±0.56

[0075] Compared with the sham operation group, * p<0.05, ** p<0.01;

[0076] Compared with the model control group, # p<0.05, ## p<0.01.

[0077] Through macroscopic observation, it was found that the structure of the sham operation group was clear, the endometrium was normal, and there was no infiltration of inflammatory cells; in the model control group, there was tissue necrosis, epithelial cell shedding, more infiltration of inflammatory cells, and some blood vessels under the endometrium were curved and thick. There was a little damage to the endometrium in the Example 1-4 groups, no bleeding, no cell infiltration, and the inflammatory reaction improved. There were inflammatory cells, blood vessel dilation, and damage to the endometrium in the Comparative Example 1-7 groups. The traditional Chinese medicine composition of the present invention has obvious improvement in the treatment of endometritis.

[0078] As can be seen from Table 1, both the example group and the comparative example group can increase the blood immunoglobulin content of rats, and the effect of the example group is particularly significant, indicating that each component of the traditional Chinese medicine composition of the present invention has a synergistic effect in the treatment of endometritis. The pharmaceutical composition of the present invention is mainly for promoting blood circulation to remove blood stasis and clearing heat and detoxifying, supplemented by functions such as regulating qi, drying dampness, and warming yang. Through the synergistic effect in many aspects, it improves the blood circulation of the endometrium, clears inflammation, regulates endocrine, and achieves the purpose of treating endometritis.

Claims

1. A pharmaceutical composition for treating endometritis, characterized in that: It comprises the following raw materials in parts by weight: 10 - 30 parts by weight of Salvia miltiorrhiza, 8 - 25 parts by weight of Rhizoma Curcumae, 8 - 25 parts by weight of Rhizoma Sparganii, 5 - 15 parts by weight of Gentiana scabra, 10 - 30 parts by weight of Taraxacum mongolicum, 10 - 25 parts by weight of Forsythia suspensa, 8 - 20 parts by weight of Ligusticum chuanxiong, 5 - 15 parts by weight of Fructus Cnidii, 8 - 20 parts by weight of Cyperus rotundus, 15 - 35 parts by weight of Astragalus membranaceus, 3 - 10 parts by weight of Glycyrrhiza uralensis.

2. The pharmaceutical composition according to claim 1, wherein: It comprises the following raw materials in parts by weight: 10 - 20 parts by weight of Salvia miltiorrhiza, 10 - 15 parts by weight of Rhizoma Curcumae, 10 - 15 parts by weight of Rhizoma Sparganii, 5 - 10 parts by weight of Gentiana scabra, 10 - 20 parts by weight of Taraxacum mongolicum, 10 - 15 parts by weight of Forsythia suspensa, 10 - 15 parts by weight of Ligusticum chuanxiong, 5 - 10 parts by weight of Fructus Cnidii, 10 - 15 parts by weight of Cyperus rotundus, 15 - 25 parts by weight of Astragalus membranaceus, 5 - 10 parts by weight of Glycyrrhiza uralensis.

3. The pharmaceutical composition according to claim 2, wherein: It comprises the following raw materials in parts by weight: 15 parts by weight of Salvia miltiorrhiza, 10 parts by weight of Rhizoma Curcumae, 10 parts by weight of Rhizoma Sparganii, 8 parts by weight of Gentiana scabra, 18 parts by weight of Taraxacum mongolicum, 15 parts by weight of Forsythia suspensa, 15 parts by weight of Ligusticum chuanxiong, 8 parts by weight of Fructus Cnidii, 12 parts by weight of Cyperus rotundus, 25 parts by weight of Astragalus membranaceus, 6 parts by weight of Glycyrrhiza uralensis.

4. A method for preparing the pharmaceutical composition for treating endometritis according to any one of claims 1 to 3, characterized in that: It comprises the following steps: Wash Salvia miltiorrhiza, Rhizoma Curcumae, Rhizoma Sparganii, Gentiana scabra, Taraxacum mongolicum, Forsythia suspensa, Ligusticum chuanxiong, Fructus Cnidii, Cyperus rotundus, Astragalus membranaceus, and Glycyrrhiza uralensis, crush them after drying, mix the crushed medicinal materials evenly, soak them in water, decoct for 2 - 3 times, filter, combine the decoction liquids, concentrate to an extract, and dry to obtain an extract powder.

5. The preparation method according to claim 4, characterized in that: Meet at least one of the following: The amount of water added is 8 - 10 times the total amount of the original medicinal materials; The soaking time is 30 - 60 minutes; The decocting time is 1 - 2 hours; The combined decoction liquid is concentrated to an extract with a relative density of 1.10 - 1.20 at 60°C; The drying is spray drying or vacuum drying.

6. Use of the pharmaceutical composition according to any one of claims 1 - 3 in the preparation of a medicament for treating endometritis.

7. The application according to claim 6, wherein: The medicament for treating endometritis is composed of the said pharmaceutical composition and a pharmaceutically acceptable carrier.

8. The application according to claim 6, wherein: The endometritis includes acute endometritis and chronic endometritis.