Biological tissue embedding machine for intensive care medicine department

Abstract

The invention relates to the technical field of medical instruments, in particular to a biological tissue embedding machine for the intensive care medicine department, which comprises a conveying frame, a rectangular groove is formed in the middle of the conveying frame, a conveying belt is fixedly mounted in the rectangular groove, mounting grooves are uniformly formed in the conveying belt, a positioning frame is fixedly mounted on the mounting grooves, and a freezing table is fixedly mounted in the middle of the conveying belt; the metal mold is placed in the positioning frame; the wax injection assembly is fixedly mounted in the middle of the upper end of the conveying frame, the lower end of the wax injection assembly comprises a primary wax injection station and a secondary wax injection station, and the wax injection assembly injects wax into the metal mold on the positioning frame to fix the biological tissue; the layering process of fixing the tissue position through primary wax injection and filling the embedding box through secondary wax injection is adopted, the freezing table is combined for rapid cooling, gradient solidification of wax liquid is achieved, a bottom supporting layer is formed through primary wax injection, the tissue is prevented from touching the bottom, a bubble-free cavity is filled through secondary wax injection, the density uniformity of wax blocks is improved, and the high-throughput requirement of the intensive care medicine department is met.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and particularly to a biological tissue embedding machine for the intensive care unit. Background Art

[0002] In pathological diagnosis and medical research, biological tissue embedding is a key pretreatment step for preparing high-quality paraffin sections. Its core objective is to retain the tissue morphological structure through paraffin infiltration and fixation, ensuring the accuracy of subsequent sectioning, staining, and microscopic observation. Traditional embedding techniques mostly rely on manual operation. Especially in high-intensity scenarios such as the intensive care unit, the following defects exist:

[0003] 1. Insufficient tissue positioning accuracy: In conventional operations, it is necessary to manually place the tissue at the center of the bottom of the metal mold and adjust the direction (such as the tumor section facing). However, due to the lack of automated positioning assistance for biological tissues, the tissue is prone to displacement during wax injection impact, resulting in the deviation of key structures during sectioning and affecting the diagnostic accuracy.

[0004] 2. Poor wax injection uniformity: Single wax injection is prone to generating cavities or bubbles due to uneven shrinkage of the wax liquid. Especially for multiple tissues co-embedded or tubular structures (such as blood vessels, intestinal tubes), incomplete wrapping or stratification is likely to occur.

[0005] 3. High risk of demolding damage: After traditional embedding, the wax block needs to be peeled off from the metal mold. However, since the tissue directly contacts the bottom of the mold, mechanical stress during demolding is likely to cause tissue tearing or edge damage.

[0006] In view of the above problems, the present invention proposes a biological tissue embedding machine for the intensive care unit to meet the special requirements of preventing biological tissues from deviating and being damaged during the embedding process. Summary of the Invention

[0007] In order to solve the problems existing in the background art, the present invention proposes a biological tissue embedding machine for the intensive care unit.

[0008] The biological tissue embedding machine for the intensive care unit provided by the present application adopts the following technical solutions:

[0009] A biological tissue embedding machine for the intensive care unit includes a conveying frame, in the middle of which there is a rectangular groove. A conveyor belt is fixedly installed in the rectangular groove. Installation grooves are evenly opened on the conveyor belt, and positioning frames are fixedly installed on the installation grooves. A freezing table is fixedly installed in the middle of the conveyor belt; the metal mold is placed inside the positioning frame; the wax injection assembly is fixedly installed in the middle of the upper end of the conveying frame. The lower end of the wax injection assembly includes a primary wax injection station and a secondary wax injection station. The distance between the primary wax injection station and the secondary wax injection station is the same as the distance between adjacent two positioning frames. When the positioning frame moves to directly below the primary wax injection station, the wax injection assembly injects wax into the metal mold on the positioning frame to achieve primary fixation of the biological tissue.

[0010] After the metal mold has completed one wax injection and moves to the secondary wax injection station, the embedding box is placed inside the positioning frame manually. The wax injection assembly injects wax into the embedding box and the inside of the metal mold again to achieve secondary fixation of the biological tissue.

[0011] The wax injection assembly includes a frame, a support, a liquid storage rack, a lifting mechanism, a conduit, a primary wax injection mechanism, and a secondary wax injection mechanism. In the middle of the upper end of the conveying rack, a frame is fixedly installed. The frame is in an L-shaped structure. Supports are fixedly installed on both sides of the frame. A liquid storage rack is fixedly installed in the middle of the frame. A lifting mechanism is installed at the front end of the frame. The primary wax injection mechanism is fixedly installed on the left side of the lower end of the lifting mechanism, and the secondary wax injection mechanism is fixedly installed on the right side of the lower end of the lifting mechanism. The primary wax injection mechanism and the secondary wax injection mechanism are respectively connected to the inside of the liquid storage rack through conduits.

[0012] Further, the positioning frame is in a rectangular frame structure. Concave grooves are provided on both sides of the positioning frame. A bearing bracket is fixedly installed inside the positioning frame. The bearing bracket is a frame structure that matches the lower end face of the metal mold. The bearing bracket is made of a heat-conducting material. The freezing table quickly cools the inside of the metal mold through the bearing bracket.

[0013] Further, the embedding box is in a rectangular frame structure. A partition is fixedly installed at the lower end inside the embedding box. Through holes allowing the wax liquid to flow through are evenly provided on the partition. Step grooves corresponding to the concave grooves are provided on both sides of the upper end of the embedding box. The inner side surface of the step groove includes a first inclined surface and a second inclined surface. The excess wax liquid inside the embedding box is discharged through the first inclined surface.

[0014] Further, the lifting mechanism includes a lifting cylinder, a cross beam frame, a mold pressing frame, and a positioning frame. Lifting cylinders are symmetrically installed at the lower end of the frame. A cross beam frame is installed between the lower ends of the lifting cylinders through a flange. Installation grooves are provided on both sides of the cross beam frame. A mold pressing frame is fixedly installed in the installation groove. The upper end of the mold pressing frame is connected to the conduit. The primary wax injection mechanism is installed inside the mold pressing frame on the left side of the cross beam frame, and the secondary wax injection mechanism is installed inside the mold pressing frame on the right side of the cross beam frame. A positioning frame is fixedly installed at the lower end of the mold pressing frame.

[0015] Further, the primary wax injection mechanism includes a wax injection pipe 1 and an alignment unit. The wax injection pipe 1 and the alignment unit are fixedly installed in the middle of the lower end of the mold pressing frame on the left side of the cross beam frame. The wax injection pipe 1 is connected to the conduit.

[0016] Further, the alignment unit includes a telescopic rod, a lifting ring, and a sampling plate. A telescopic rod is installed on the mold pressing frame. The extended length of the telescopic rod is adjustable. A lifting ring is fixedly installed on the telescopic rod. A sampling plate is fixedly installed at the lower end of the telescopic rod;

[0017] The sampling plate includes a fixed end and a flipping end, which are connected by a pin shaft. The fixed end is installed at the lower end of the telescopic rod, and a connecting rod is installed between the flipping end and the lifting ring through a pin shaft. Negative pressure adsorption holes are evenly installed at the lower end of the flipping end.

[0018] Furthermore, the secondary wax injection mechanism includes a second wax injection pipe and a collection unit. The second wax injection pipe is fixedly installed in the middle of the lower end of the mold pressing frame on the left side of the cross beam frame. The second wax injection pipe is communicated with the conduit, and collection units are installed on both sides of the lower end of the mold pressing frame on the left side of the cross beam frame. The collection unit is used to collect the excess wax liquid during secondary wax injection.

[0019] Furthermore, the collection unit includes a collection frame and a guiding frame. The collection frame is installed at the lower end of the mold pressing frame. The inside of the collection frame is a hollow structure. The collection frame is matched with the concave grooves on both sides of the positioning frame. A guiding frame is fixedly installed inside the collection frame, and communication holes are evenly arranged at the connection between the guiding frame and the collection frame.

[0020] Furthermore, the guiding frame is of an inclined structure. The lower end surface of the guiding frame fits with the first inclined surface of the inner side of the stepped groove, and the upper end surface of the guiding frame is parallel to the second inclined surface of the inner side of the stepped groove.

[0021] Furthermore, pressing frames are symmetrically installed at the lower end inside the positioning frame on the left side of the cross beam frame. The pressing frames are used to press the upper end of the embedding cassette so that the lower end of the embedding cassette can be closely attached to the metal mold.

[0022] Beneficial effects

[0023] Compared with the prior art, the present invention provides a biological tissue embedding machine for the intensive care unit, which has the following beneficial effects:

[0024] 1. In this invention, through the negative pressure adsorption and flipping functions of the alignment unit, combined with the linkage control of the telescopic rod and the lifting ring, the tissue can be automatically adjusted horizontally or vertically to ensure that it is in the center position of the metal mold, avoiding the deviation caused by manual operation, being able to adapt to different biological tissues, and improving the accuracy of pathological diagnosis.

[0025] 2. In this invention, a layered process of using primary wax injection to fix the tissue position and secondary wax injection to fill the embedding cassette is adopted. Combined with the rapid cooling of the freezing table through the heat-conducting support bracket, gradient solidification of the wax liquid is achieved. The primary wax injection forms a bottom support layer to prevent the tissue from touching the bottom. The secondary wax injection fills the bubble-free cavity, improving the density uniformity of the wax block and making the slices complete, meeting the high-throughput requirements of the intensive care unit.

[0026] 3. In this invention, the collection unit of the secondary wax injection mechanism cooperates with the stepped groove through the guiding frame, and combined with the negative pressure adsorption system to recover the excess wax liquid, reducing wax liquid waste. After the wax liquid solidifies inside the embedding cassette, there is no convex structure on the surface, which can be directly adapted to the pathological slicer, eliminating the manual trimming step.

[0027] 4. In this invention, the embedding cassette and the metal mold are connected through the through holes of the partition plate. After the wax liquid solidifies, an integral structure is formed, and it can be directly installed on the microtome without demolding, eliminating the risk of tissue tearing caused by traditional demolding, especially protecting fragile samples. The integrated design of the wax block and the embedding cassette avoids the fragmentation of the wax block during the transfer process. Description of the Drawings

[0028] The present invention will be further described below in conjunction with the drawings and embodiments.

[0029] Figure 1 It is a three-dimensional structure schematic diagram of the present application.

[0030] Figure 2 It is a three-dimensional structure schematic diagram between the positioning frame and the metal mold of the present application.

[0031] Figure 3 It is a three-dimensional structure schematic diagram between the positioning frame and the embedding cassette of the present application.

[0032] Figure 4 It is a three-dimensional structure schematic diagram between the wax injection assembly and the positioning frame of the present application.

[0033] Figure 5 It is a three-dimensional structure schematic diagram among the positioning frame, the metal mold, the lifting mechanism and the embedding cassette of the present application.

[0034] Figure 6 It is a bottom view among the crossbeam frame, the mold pressing frame, the positioning frame, the primary wax injection mechanism and the secondary wax injection mechanism of the present application.

[0035] Figure 7 It is a three-dimensional structure schematic diagram among the mold pressing frame, the positioning frame and the primary wax injection mechanism of the present application.

[0036] Figure 8 It is a three-dimensional structure schematic diagram of the alignment unit of the present application.

[0037] Figure 9 It is a three-dimensional structure schematic diagram between the positioning frame and the secondary wax injection mechanism of the present application.

[0038] Figure 10 It is a three-dimensional structure schematic diagram of the collection unit of the present application.

[0039] Figure 11 It is a sectional structure schematic diagram among the positioning frame, the metal mold, the lifting mechanism, the conduit and the secondary wax injection mechanism of the present application.

[0040] Description of reference numerals in the drawings: 1. Conveyor rack; 11. Conveyor belt; 12. Positioning rack; 121. Concave groove; 122. Bracket; 2. Metal mold; 3. Wax injection assembly; 31. Frame; 32. Support member; 33. Liquid storage rack; 34. Lifting mechanism; 341. Lifting cylinder; 342. Cross beam rack; 343. Mold pressing rack; 344. Positioning frame; 3441. Pressing frame; 35. Conduit; 36. Primary wax injection mechanism; 361. First wax injection pipe; 362. Alignment unit; 3621. Telescopic rod; 3622. Lifting ring; 3623. Sampling plate; 3624. Connecting rod; 37. Secondary wax injection mechanism; 371. Second wax injection pipe; 372. Collection unit; 3721. Collection rack; 3722. Guide rack; 4. Embedding cassette; 41. Partition; 42. Step groove. Detailed implementation manners

[0041] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0042] Please refer to Figures 1-11 , a biological tissue embedding machine for the intensive care unit provided in the embodiments of the present invention includes a conveyor rack 1, a rectangular groove is provided in the middle thereof, a conveyor belt 11 is fixedly installed in the rectangular groove, mounting grooves are uniformly formed in the conveyor belt 11, a positioning rack 12 is fixedly installed on the mounting grooves, and a freezing table is fixedly installed in the middle of the conveyor belt 11; a metal mold 2 is placed inside the positioning rack 12; a wax injection assembly 3 is fixedly installed in the middle of the upper end of the conveyor rack 1, the lower end of the wax injection assembly 3 includes a primary wax injection station and a secondary wax injection station, and the distance between the primary wax injection station and the secondary wax injection station is the same as the distance between two adjacent positioning racks 12. When the positioning rack 12 moves to directly below the primary wax injection station, the wax injection assembly 3 injects wax into the metal mold 2 on the positioning rack 12 to achieve primary fixation of the biological tissue.

[0043] The metal molds 2 need to be placed one by one inside the positioning racks 12 on the conveyor belt 11. The conveyor belt 11 drives the positioning racks 12 to move intermittently. When the positioning rack 12 first moves to the primary wax injection station, the biological tissue is manually placed inside the metal mold 2. The biological tissue needs to be ensured to be at the exact center position at the bottom of the metal mold. The wax injection assembly 3 first fixes the biological tissue inside the metal mold to prevent the biological tissue from shifting during subsequent wax injection, so that the biological tissue can be at the center position of the solidified wax block, which is beneficial for subsequent tissue sectioning.

[0044] After the metal mold 2 has completed one wax injection and moves to the secondary wax injection station, the embedding box 4 is placed inside the positioning frame 12 manually. The wax injection assembly 3 injects wax into the embedding box 4 and the inside of the metal mold 2 again to achieve secondary fixation of the biological tissue. Since the distance between the primary wax injection station and the secondary wax injection station is the same as the distance between two adjacent positioning frames 12, that is, when the wax injection assembly 3 descends once, both primary and secondary wax injections can be achieved.

[0045] The biological tissue after one wax injection is fixed inside the metal mold 2, and there is a certain space between the lower end of the biological tissue and the inside of the metal mold 2 to prevent the biological tissue from touching the bottom completely. After the secondary wax injection is completed, the wax liquid inside the embedding box 4 and the wax liquid inside the metal mold 2 solidify to form a complete whole. Then, the metal mold 2 is demolded, and the biological tissue is thus fixed below the embedding box 4. The embedding box 4 can be installed on a microtome to slice the solidified biological tissue. When slicing, it is not necessary to remove the solidified wax block from the inside of the embedding box 4, which improves the convenience of subsequent slicing.

[0046] Refer to Figures 4-6 As shown in the figure, the wax injection assembly 3 includes a frame 31, a support 32, a liquid storage rack 33, a lifting mechanism 34, a conduit 35, a primary wax injection mechanism 36, and a secondary wax injection mechanism 37. The middle part of the upper end of the conveying rack 1 is fixedly installed with a frame 31. The frame 31 is in an L-shaped structure. The support 32 is fixedly installed on both sides of the frame 31. The liquid storage rack 33 is fixedly installed in the middle of the frame 31. The lifting mechanism 34 is installed at the front end of the frame 31. The primary wax injection mechanism 36 is fixedly installed on the left side of the lower end of the lifting mechanism 34, and the secondary wax injection mechanism 37 is fixedly installed on the right side of the lower end of the lifting mechanism 34. The primary wax injection mechanism 36 and the secondary wax injection mechanism 37 are respectively connected to the inside of the liquid storage rack 33 through the conduit 35.

[0047] In the above technical solution, the wax liquid is stored inside the liquid storage rack 33. When the positioning frame 12 moves to directly below the primary wax injection mechanism 36 and the secondary wax injection mechanism 37, after the biological tissue and the embedding box 4 are placed manually, the lifting mechanism 34 drives the primary wax injection mechanism 36 and the secondary wax injection mechanism 37 to move downward, so that the primary wax injection mechanism 36 and the secondary wax injection mechanism 37 can be in close contact with the positioning frame 12. At the same time, the wax liquid inside the liquid storage rack 33 is injected into the inside of the positioning frame 12 through the conduit 35 from the primary wax injection mechanism 36 and the secondary wax injection mechanism 37 respectively. Then, the lifting mechanism 34 drives the primary wax injection mechanism 36 and the secondary wax injection mechanism 37 to rise, thus completing the wax injection process. The primary wax injection mechanism 36 performs primary wax injection on the biological tissue inside the positioning frame 12. After the positioning frame 12 has completed the primary wax injection, it continues to move forward, and the secondary wax injection mechanism 37 performs secondary wax injection on the inside of the positioning frame 12 that has already been wax-injected.

[0048] Refer to Figure 4As shown, the support member 32 is a diagonal bracing structure. The support member 32 can play a role in fixedly supporting the frame 31, ensuring that the lifting mechanism 34 inside the frame 31 can move up and down stably and preventing shaking.

[0049] It should be noted that a liquid injection port is provided at the upper end of the liquid storage rack 33. A detachable sealing plate is installed on the liquid injection port, and handles are symmetrically arranged on the sealing plate. By removing the seal, wax liquid can be added to the inside of the liquid storage rack 33. The liquid storage rack 33 and the conduit 35 are made of heat-insulating materials, which can reduce the phenomenon of solidification of the molten wax liquid, keep the wax liquid at a set temperature all the time, and improve the embedding effect of biological tissues after wax injection.

[0050] Refer to Figure 2 and Figure 11 As shown, as a preferred technical solution of this embodiment, the positioning frame 12 has a rectangular frame structure. Concave grooves 121 are provided on both sides of the positioning frame 12. A supporting bracket 122 is fixedly installed inside the positioning frame 12. The supporting bracket 122 has a frame structure that matches the lower end face of the metal mold 2. The supporting bracket 122 is made of a heat-conducting material, and the freezing table rapidly cools the inside of the metal mold 2 through the supporting bracket 122.

[0051] In the above technical solution, the metal mold 2 is placed inside the positioning frame 12, and the supporting bracket 122 can support and limit the metal mold 2. When the wax liquid is injected into the metal mold 2, since the supporting bracket 122 is made of a heat-conducting material, the freezing table can rapidly cool the wax liquid inside the metal mold 2 through the supporting bracket 122, which is convenient for the rapid fixation of biological tissues.

[0052] Refer to Figure 3 and Figure 11 As shown, as a preferred technical solution of this embodiment, the embedding box 4 has a rectangular frame structure. A partition 41 is fixedly installed at the lower end inside the embedding box 4. Through holes allowing the wax liquid to flow through are evenly provided on the partition 41. Step grooves 42 corresponding to the concave grooves 121 are provided on both sides at the upper end of the embedding box 4. The inner side surface of the step groove 42 includes a first inclined surface and a second inclined surface. The excess wax liquid inside the embedding box 4 is discharged through the first inclined surface.

[0053] In the above technical solution, when the positioning frame 12 moves below the secondary wax injection station, the embedding cassette 4 is manually placed inside the positioning frame 12. There is a connection between the inside of the embedding cassette 4 and the metal mold 2. The secondary wax injection mechanism 37 injects wax into the inside of the embedding cassette 4. The wax liquid first flows into the inside of the metal mold 2 through the through holes on the partition plate 41. When the inside of the metal mold 2 is filled, the wax liquid will stay inside the embedding cassette 4. When a large amount of wax liquid is injected, the wax liquid will flow outwards along the stepped groove 42, preventing the wax liquid from being injected too much inside the embedding cassette 4, which may cause the solidified wax liquid to form an upward convex structure at the upper end of the embedding cassette 4. The convex structure will exceed the upper surface of the embedding cassette 4, and the convex structure affects the installation of the embedding cassette 4 on the pathological slicing machine.

[0054] Refer to Figures 4-6 As shown in the figure, as a preferred technical solution of this embodiment, the lifting mechanism 34 includes a lifting cylinder 341, a cross beam frame 342, a mold pressing frame 343, and a positioning frame 344. The lifting cylinders 341 are symmetrically installed at the lower end of the frame 31. The cross beam frame 342 is installed between the lower ends of the lifting cylinders 341 through a flange. Installation grooves are provided on both sides of the cross beam frame 342, and the mold pressing frames 343 are fixedly installed in the installation grooves. The upper end of the mold pressing frame 343 is connected to the conduit 35. The primary wax injection mechanism 36 is installed inside the mold pressing frame 343 on the left side of the cross beam frame 342, and the secondary wax injection mechanism 37 is installed inside the mold pressing frame 343 on the right side of the cross beam frame 342. The positioning frame 344 is fixedly installed at the lower end of the mold pressing frame 343.

[0055] In the above technical solution, when the positioning frame 12 moves directly below the primary wax injection mechanism 36 and the secondary wax injection mechanism 37, the lifting cylinder 341 drives the two mold pressing frames 343 and the two positioning frames 344 to move downward synchronously through the cross beam frame 342, so that the primary wax injection mechanism 36 and the secondary wax injection mechanism 37 can accurately inject the wax liquid into the metal mold 2 on the positioning frame 12.

[0056] The positioning insertion rods are uniformly arranged at the lower end of the positioning frame 344, and the positioning holes corresponding to the positioning insertion rods are uniformly arranged on the positioning frame 12 (the positioning insertion rods and the positioning holes are not marked in the attached figure). The positioning frame 344 and the positioning frame 12 are accurately aligned through the cooperation of the positioning insertion rods and the positioning holes.

[0057] Refer to Figures 6-7 As shown in the figure, as a preferred technical solution of this embodiment, the primary wax injection mechanism 36 includes a wax injection pipe 361 and an alignment unit 362. The wax injection pipe 361 and the alignment unit 362 are fixedly installed in the middle of the lower end of the mold pressing frame 343 on the left side of the cross beam frame 342. The wax injection pipe 361 is connected to the conduit 35.

[0058] In the actual use process, when performing wax injection on biological tissue for the first time, first, manually place the biological tissue inside the metal mold, aligning the alignment unit 362 with the center of the lower end of the metal mold 2. Then, the alignment unit 362 moves downward to adsorb and fix the biological tissue and then lifts upward, leaving a certain distance between the lower end of the biological tissue and the bottom of the metal mold 2 to prevent the wax-injected biological tissue from touching the bottom. Then, inject a small amount of wax liquid into the metal mold 2 through the first wax injection tube 361 to fix the biological tissue.

[0059] Continue to refer to Figure 8 As shown, as a preferred technical solution of this embodiment, the alignment unit 362 includes a telescopic rod 3621, a lifting ring 3622, and a sampling plate 3623. The telescopic rod 3621 is installed on the press mold frame 343, and the extended length of the telescopic rod 3621 is adjustable. A lifting ring 3622 is fixedly installed on the telescopic rod 3621. The lifting ring 3622 further includes a telescopic airbag, which is installed on the telescopic rod 3621. The lifting ring 3622 is fixedly installed at the lower end of the telescopic airbag. By injecting and exhausting air into the telescopic airbag, the function of the lifting ring 3622 moving up and down can be realized. The lower end of the telescopic rod 3621 is fixedly installed with a sampling plate 3623. The sampling plate 3623 includes a fixed end and a flipping end, which are connected by a pin shaft between the fixed end and the flipping end. The fixed end is installed at the lower end of the telescopic rod 3621, and a connecting rod is installed between the flipping end and the lifting ring 3622 through a pin shaft. Negative pressure adsorption holes are evenly installed at the lower end of the flipping end.

[0060] In the actual use process, when the biological tissue needs to be embedded, the telescopic rod 3621 drives the sampling plate 3623 to move downward to adsorb and fix the biological tissue, and then the telescopic rod 3621 drives the biological tissue to move upward to avoid the biological tissue touching the bottom. When the biological tissue needs to be placed vertically (such as blood vessels, intestinal tubes), the lower end of the flipping end adsorbs and fixes the biological tissue, and the lifting ring 3622 drives the flipping end to flip upward through the connecting rod, so that the flipping end changes from a horizontal state to a vertical state, thus meeting the need for vertical embedding.

[0061] It should be noted that when the biological tissue needs to be placed horizontally, the telescopic rod 3621 first drives the sampling plate 3623 to suck the biological tissue. Then, lift the biological tissue upward, first inject wax into the metal mold 2, and wait until the wax liquid is about to solidify. The telescopic rod 3621 drives the biological tissue to descend through the sampling plate 3623, so that the lower end surface of the biological tissue contacts the wax liquid. At this time, the adsorption holes at the lower end of the flipping end stop adsorbing to avoid sucking the wax liquid into. At the same time, the telescopic rod 3621 continues to drive the sampling plate 3623 to move downward, so that the sampling plate 3623 can press the biological tissue to ensure that the lower end of the biological tissue can be completely solidified into the wax liquid.

[0062] When the biological tissue needs to be placed vertically, the telescopic rod 3621 first drives the sampling plate 3623 to suck the biological tissue. Since part of the biological tissue is below the fixed end and part is below the flipping end, when the flipping end flips, the biological tissue below the fixed end will stand up following the flipping end, and the lower end of the biological tissue will extend below the fixed end. At this time, by first injecting wax into the metal mold 2, the wax liquid first embeds and solidifies the biological tissue below the fixed end. Then, the flipping end stops adsorbing, and the entire biological tissue is vertically embedded by gradually moving the flipping end upward.

[0063] Refer to Figure 9 As shown, as a preferred technical solution of this embodiment, the secondary wax injection mechanism 37 includes a second wax injection pipe 371 and a collection unit 372. The second wax injection pipe 371 is fixedly installed in the middle of the lower end of the mold pressing frame 343 on the left side of the cross beam frame 342. The second wax injection pipe 371 is communicated with the conduit 35, and the collection unit 372 is installed on both sides of the lower end of the mold pressing frame 343 on the left side of the cross beam frame 342. The collection unit 372 is used to collect the excess wax liquid during secondary wax injection.

[0064] During actual use, when the metal mold after one wax injection moves below the secondary wax injection mechanism 37, the lifting mechanism 34 drives the second wax injection pipe 371 and the collection unit 372 to descend. The collection unit 372 can be accurately inserted into the recessed grooves 121 on both sides of the positioning frame 12, and the inner side of the collection unit 372 just cooperates with the stepped grooves 42 on both sides of the embedding box 4. Then, wax is injected into the embedding box through the second wax injection pipe 371. When the amount of wax liquid injected is excessive, the wax liquid will flow into the collection unit 372 along the stepped grooves 42 on both sides of the embedding box 4. The inside of the collection unit 372 is a negative pressure structure, so that the excess wax liquid will be collected and stored through the collection unit 372, avoiding waste of wax liquid.

[0065] Continue to refer to Figure 10 As shown, as a preferred technical solution of this embodiment, the collection unit 372 includes a collection frame 3721 and a guide frame 3722. The collection frame 3721 is installed at the lower end of the mold pressing frame 343. The inside of the collection frame 3721 is a hollow structure. The collection frame 3721 cooperates with the recessed grooves 121 on both sides of the positioning frame 12. A guide frame 3722 is fixedly installed inside the collection frame 3721. Communication holes are evenly arranged at the connection between the guide frame 3722 and the collection frame 3721.

[0066] Refer to Figure 10 As shown, as a preferred technical solution of this embodiment, the guide frame 3722 is an inclined structure. The lower end surface of the guide frame 3722 fits with the first inclined surface on the inner side of the stepped groove 42, and the upper end surface of the guide frame 3722 is parallel to the second inclined surface on the inner side of the stepped groove 42.

[0067] In actual use, when the collecting rack 3721 is lowered and inserted into the recessed grooves 121 on both sides of the positioning rack 12, the guide rack 3722 just cooperates with the stepped grooves 42, and the upper end surface of the guide rack 3722 is parallel to the second inclined surface of the inner side surface of the stepped grooves 42, so that when there is too much wax liquid, the wax liquid will enter the upper end surface of the guide rack 3722 through the second inclined surface. At the same time, the negative pressure structure inside the collecting rack 3721 will absorb the wax liquid, so that the wax liquid will enter the collecting rack 3721 through the connecting hole, preventing the wax liquid from flowing into the side wall of the embedding box, avoiding the need for secondary cleaning in the future.

[0068] See also Figure 11 As shown, as a preferred technical solution of this embodiment, a clamping frame 3441 is symmetrically installed at the lower end of the inner part of the positioning frame 344 on the left side of the crossbeam frame 342, and the clamping frame 3441 is used to clamp the upper end of the embedding box 4 so that the lower end of the embedding box 4 can be tightly attached to the metal mold 2.

[0069] In actual use, when the lifting mechanism 34 moves downward, the pressing frame 3441 inside the positioning frame 344 will press the upper end of the embedding box 4 so that the lower end of the embedding box 4 can be tightly attached to the surface of the metal mold 2 to prevent a gap from being generated between the embedding box 4 and the metal mold 2.

[0070] In combination with the above structure, the present invention provides a biological tissue embedding machine for critical care medicine, which is implemented according to the following steps when injecting wax into tissues for embedding:

[0071] S1. Metal mold loading and initial positioning

[0072] Place the metal molds 2 one by one inside the positioning frame 12 of the conveyor belt 11, and ensure that the metal mold 2 is firmly supported by the support frame 122; start the conveyor belt 11 to make it move intermittently, and transport the first positioning frame 12 to the bottom of the primary wax injection station; the freezing table pre-cools the metal mold 2 through the support frame 122 made of heat-conductive material, creating conditions for the rapid solidification of the subsequent wax injection.

[0073] S2. Biological tissue alignment and one-time wax injection fixation

[0074] The operator places the biological tissue precisely in the center area of the bottom of the metal mold 2.

[0075] The biological tissue is placed horizontally: the alignment unit 362 of the primary wax injection mechanism 36 is started, and the telescopic rod 3621 drives the sampling plate 3623 to descend, adsorb the biological tissue through the negative pressure adsorption holes, and lift it to a set distance from the bottom of the mold to avoid touching the bottom.

[0076] Biological tissue is placed vertically (such as blood vessels / intestinal tubes): after the flip end absorbs the tissue, the lifting ring 3622 drives the flip end to rotate 90 degrees upward through the connecting rod, so that the biological tissue is in a vertical state.

[0077] Primary wax injection:

[0078] The wax injection pipe 361 of the primary wax injection mechanism 36 injects a small amount of molten wax liquid at 56 - 60 °C into the metal mold 2, covering the bottom of the tissue and preliminarily fixing it; the freezing table accelerates the solidification of the wax liquid to form a support layer, ensuring the stable position of the tissue.

[0079] S3. Transport to the secondary wax injection station and installation of the embedding cassette

[0080] After the primary wax injection is completed, the conveyor belt 11 moves the positioning frame 12 to directly below the secondary wax injection station.

[0081] Placement of the embedding cassette: Manually install the embedding cassette 4 inside the positioning frame 12, aligning its stepped groove 42 with the recessed groove 121 of the positioning frame, and the through holes on the partition plate 41 are in communication with the inside of the metal mold 2.

[0082] Pressing and fixing: The lifting mechanism 34 drives the pressing frame 3441 to press down, ensuring that the embedding cassette 4 is in close contact with the metal mold 2 to prevent wax leakage during wax injection.

[0083] S4. Secondary wax injection and recovery of excess wax liquid

[0084] Secondary wax injection:

[0085] The wax injection pipe 371 of the secondary wax injection mechanism 37 injects a sufficient amount of wax liquid into the embedding cassette 4 and the metal mold 2. The wax liquid uniformly flows into the inside of the metal mold 2 through the through holes in the partition plate 41, filling the remaining space of the metal mold 2 and fusing with the primary wax injection layer.

[0086] Wax liquid recovery:

[0087] The excess wax liquid overflows along the stepped grooves 42 on both sides of the embedding cassette 4. The negative pressure structure inside the collection rack 3721 adsorbs the wax liquid, causing the wax liquid to enter the inside of the collection rack 3721 through the communication holes, preventing the wax liquid from flowing onto the side wall of the embedding cassette and avoiding the formation of protrusions after the wax liquid solidifies, which may affect the installation of the microtome. The freezing table continues to cool down to accelerate the solidification and forming of the overall wax block.

[0088] S5. Demolding and preparation for sectioning

[0089] Demolding operation:

[0090] After the wax liquid is completely solidified, manually remove the metal mold 2 from the positioning frame 12 and gently tap it for demolding. The biological tissue and the wax block at the bottom of the embedding cassette 4 form an integral structure, eliminating the need for additional transfer.

[0091] Preparation for sectioning: Directly install the embedding cassette 4 onto the microtome, using the pure wax layer reserved at the bottom to prevent the tissue from touching the bottom, facilitating subsequent sectioning processing.

[0092] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above-described exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, in any respect, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention. Any reference signs in the claims should not be construed as limiting the claims involved.

Claims

1. A biological tissue embedding machine for the intensive care unit, characterized in that, Including: A conveying rack (1), in the middle of which there is a rectangular groove, a conveyor belt (11) is fixedly installed in the rectangular groove, mounting grooves are evenly formed in the conveyor belt (11), a positioning rack (12) is fixedly installed on the mounting grooves, and a freezing table is fixedly installed in the middle of the conveyor belt (11); A metal mold (2), which is placed inside the positioning rack (12); A wax injection assembly (3), which is fixedly installed in the middle of the upper end of the conveying rack (1). The lower end of the wax injection assembly (3) includes a primary wax injection station and a secondary wax injection station. The distance between the primary wax injection station and the secondary wax injection station is the same as the distance between two adjacent positioning racks (12). When the positioning rack (12) moves to directly below the primary wax injection station, the wax injection assembly (3) injects wax into the metal mold (2) on the positioning rack (12) to achieve the primary fixation of biological tissue; After the metal mold (2) after the primary wax injection moves to the secondary wax injection station, an embedding box (4) is placed inside the positioning rack (12) manually, and the wax injection assembly (3) injects wax into the embedding box (4) and the metal mold (2) again to achieve the secondary fixation of biological tissue; The wax injection assembly (3) includes a frame (31), a support (32), a liquid storage rack (33), a lifting mechanism (34), a conduit (35), a primary wax injection mechanism (36) and a secondary wax injection mechanism (37). The frame (31) is fixedly installed in the middle of the upper end of the conveying rack (1). The frame (31) has an L-shaped structure. Supports (32) are fixedly installed on both sides of the frame (31). The liquid storage rack (33) is fixedly installed in the middle of the frame (31). A lifting mechanism (34) is installed at the front end of the frame (31). The primary wax injection mechanism (36) is fixedly installed on the left side of the lower end of the lifting mechanism (34), and the secondary wax injection mechanism (37) is fixedly installed on the right side of the lower end of the lifting mechanism (34). The primary wax injection mechanism (36) and the secondary wax injection mechanism (37) are respectively connected to the inside of the liquid storage rack (33) through the conduit (35).

2. The biological tissue embedding machine for the intensive care unit according to claim 1, characterized in that: The positioning rack (12) has a rectangular frame structure. Concave grooves (121) are formed on both sides of the positioning rack (12). A supporting bracket (122) is fixedly installed inside the positioning rack (12). The supporting bracket (122) has a frame structure matching the lower end face of the metal mold (2). The supporting bracket (122) is made of a heat-conducting material. The freezing table rapidly cools the inside of the metal mold (2) through the supporting bracket (122).

3. The biological tissue embedding machine for the intensive care unit according to claim 2, characterized in that: The embedding box (4) has a rectangular frame structure. A partition (41) is fixedly installed at the lower end inside the embedding box (4). Through holes allowing the wax liquid to flow through are evenly formed in the partition (41). Step grooves (42) corresponding to the concave grooves (121) are arranged on both sides of the upper end of the embedding box (4). The inner side surface of the step groove (42) includes a first inclined surface and a second inclined surface. The excess wax liquid inside the embedding box (4) is discharged through the first inclined surface.

4. A biological tissue embedding machine for the intensive care unit according to claim 3, characterized in that: The lifting mechanism (34) includes a lifting cylinder (341), a crossbeam frame (342), a mold pressing frame (343) and a positioning frame (344). The lifting cylinders (341) are symmetrically installed at the lower end of the frame (31). A crossbeam frame (342) is installed between the lower ends of the lifting cylinders (341) through a flange. Installation grooves are provided on both sides of the crossbeam frame (342), and a mold pressing frame (343) is fixedly installed in the installation grooves. The upper end of the mold pressing frame (343) is communicated with the conduit (35). A primary wax injection mechanism (36) is installed inside the mold pressing frame (343) on the left side of the crossbeam frame (342), and a secondary wax injection mechanism (37) is installed inside the mold pressing frame (343) on the right side of the crossbeam frame (342). A positioning frame (344) is fixedly installed at the lower end of the mold pressing frame (343).

5. The biological tissue embedding machine for the intensive care unit according to claim 4, wherein: The primary wax injection mechanism (36) includes a first wax injection pipe (361) and an alignment unit (362). The first wax injection pipe (361) and the alignment unit (362) are fixedly installed in the middle of the lower end of the mold pressing frame (343) on the left side of the crossbeam frame (342). The first wax injection pipe (361) is communicated with the conduit (35).

6. The biological tissue embedding machine for the intensive care unit according to claim 5, characterized in that: The alignment unit (362) includes a telescopic rod (3621), a lifting ring (3622) and a sampling plate (3623). A telescopic rod (3621) is installed on the mold pressing frame (343). The extending length of the telescopic rod (3621) is adjustable. A lifting ring (3622) is fixedly installed on the telescopic rod (3621). A sampling plate (3623) is fixedly installed at the lower end of the telescopic rod (3621). The sampling plate (3623) includes a fixed end and a flipping end. The fixed end and the flipping end are connected by a pin shaft. The fixed end is installed at the lower end of the telescopic rod (3621). A connecting rod is installed between the flipping end and the lifting ring (3622) through a pin shaft. Negative pressure adsorption holes are evenly installed at the lower end of the flipping end.

7. A biological tissue embedding machine for the intensive care unit according to claim 6, characterized in that: The secondary wax injection mechanism (37) includes a second wax injection pipe (371) and a collection unit (372). The second wax injection pipe (371) is fixedly installed in the middle of the lower end of the mold pressing frame (343) on the left side of the crossbeam frame (342). The second wax injection pipe (371) is communicated with the conduit (35). Collection units (372) are installed on both sides of the lower end of the mold pressing frame (343) on the left side of the crossbeam frame (342). The collection unit (372) is used to collect the excess wax liquid during secondary wax injection.

8. A biological tissue embedding machine for the intensive care unit according to claim 7, characterized in that: The collection unit (372) includes a collection frame (3721) and a guiding frame (3722). The collection frame (3721) is installed at the lower end of the mold pressing frame (343). The inside of the collection frame (3721) is a hollow structure. The collection frame (3721) is matched with the concave grooves (121) on both sides of the positioning frame (12). A guiding frame (3722) is fixedly installed inside the collection frame (3721). Communication holes are evenly arranged at the connection between the guiding frame (3722) and the collection frame (3721).

9. The biological tissue embedding machine for the intensive care unit according to claim 8, characterized in that: The guiding frame (3722) is of an inclined structure. The lower end face of the guiding frame (3722) fits with the first inclined surface of the inner side face of the stepped groove (42). The upper end face of the guiding frame (3722) is parallel to the second inclined surface of the inner side face of the stepped groove (42).

10. A biological tissue embedding machine for the intensive care unit according to claim 9, characterized in that: At the lower end inside the positioning frame (344) on the left side of the cross beam frame (342), pressing frames (3441) are symmetrically installed. The pressing frames (3441) are used to press the upper end of the embedding box (4) so that the lower end of the embedding box (4) can be in close contact with the metal mold (2).

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