Application of pinolenic acid in promoting growth of panax notoginseng and preventing and treating diseases

Abstract

The invention relates to the technical field of plant disease control, in particular to application of pinolenic acid to panax notoginseng growth promotion and disease control. In one aspect, the invention provides the application of pinolenic acid in prevention and treatment of panax notoginseng root rot, pinolenic acid inhibits phytopathogen fusarium and rust rot fungi, and increases the growth of beneficial bacteria trichoderma, penicillium and mortierella. In another aspect, the invention provides the application of the pinolenic acid in promoting the growth of the panax notoginseng, and the pinolenic acid improves the overground single plant dry weight of the panax notoginseng, improves the underground single plant dry weight, increases the fibrous root length of the panax notoginseng, increases the fibrous root surface area of the panax notoginseng and increases the fibrous root volume of the panax notoginseng.

Description

Technical Field

[0001] The present invention relates to the technical field of plant disease control, and in particular to the application of pinolenic acid in promoting the growth of Panax notoginseng and disease control. Background Art

[0002] Pinolenic acid (16833 - 54 - 8), molecular formula: C 18 H 30 O2, molecular weight: 278.43, derived from the root exudates of pine trees.

[0003] The problem of continuous cropping obstacle of Panax notoginseng is becoming increasingly prominent in Yunnan Province, the main producing area. The main manifestations are the decline of soil quality, resulting in the reduction of the germination rate of Panax notoginseng seeds, the deterioration of plant growth conditions, and the high incidence of root rot disease. In severe cases, the yield can be sharply reduced or even no harvest. To restore soil suitability, long-term crop rotation is required, but the actual operation is difficult. Although chemical pesticides can temporarily control pests and diseases, they are unstable and may exacerbate environmental pollution. Although methods such as soil fumigation are effective, they will damage the quality of medicinal materials and soil health. Facing the improvement of the quality requirements of Panax notoginseng and the complexity of root rot disease, the existing control measures seem inadequate, and there is an urgent need to develop new technologies to promote the growth of Panax notoginseng and effectively control root rot disease. Summary of the Invention

[0004] The present invention provides the application of pinolenic acid in promoting the growth of Panax notoginseng and disease control, so as to solve the defect that the existing technology lacks methods for promoting the growth of Panax notoginseng and controlling root rot disease. In particular, it provides the application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in promoting beneficial rhizosphere microorganisms of Penicillium, Mortierella and Trichoderma in Panax notoginseng and controlling root rot disease of Panax notoginseng caused by Fusarium and Cylindrocarpon.

[0005] The present invention provides a plant pathogen bacteriostatic agent, which comprises pinolenic acid or its derivatives or its pharmaceutically acceptable salts.

[0006] Preferably, the plant pathogen is Fusarium or Cylindrocarpon. The bacteriostatic agent of the present invention can control root rot disease of Panax notoginseng.

[0007] The present invention provides a Cylindrocarpon bacteriostatic agent, which comprises pinolenic acid or its derivatives or its pharmaceutically acceptable salts.

[0008] The present invention provides a Fusarium bacteriostatic agent, which comprises pinolenic acid or its derivatives or its pharmaceutically acceptable salts.

[0009] The present invention provides the application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in controlling root rot disease of Panax notoginseng.

[0010] The present invention provides the use of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof in preventing and treating Panax notoginseng root rot caused by Fusarium and Cryptosporiopsis.

[0011] Preferably, the pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof inhibit plant pathogenic fungi Fusarium and / or Cryptosporiopsis, and at the same time increase the growth of beneficial fungi Trichoderma, Penicillium and / or Mortierella.

[0012] According to the use of inhibiting Fusarium provided by the present invention, the dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is not higher than 0.001 ppm.

[0013] According to the use of inhibiting Fusarium provided by the present invention, the optimal dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is 0.001 ppm.

[0014] According to the use of inhibiting Fusarium provided by the present invention, the dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is below 0.001 ppm.

[0015] According to the use of inhibiting Cryptosporiopsis provided by the present invention, the dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is not lower than 0.0001 ppm.

[0016] According to the use of inhibiting Cryptosporiopsis provided by the present invention, the optimal dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is 0.0001 ppm - 0.1 ppm. The use of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof in prevention and treatment.

[0017] According to the use of inhibiting Cryptosporiopsis provided by the present invention, the dosage concentration of pinolenic acid or its derivatives or pharmaceutically acceptable salts thereof is above 0.0001 ppm.

[0018] The present invention proves through antibacterial activity determination that pinolenic acid has good inhibitory activity against Fusarium of Panax notoginseng and Cryptosporiopsis. As a new type of fungicide, pinolenic acid meets the requirements of chemical control of plant diseases.

[0019] The highest concentration of pinolenic acid inhibiting Fusarium is 0.001 ppm.

[0020] When the concentration of pinolenic acid is 0.001 ppm, the mycelial growth of Fusarium is significantly lower than that of the control group, indicating that when the concentration of pinolenic acid is low, the occurrence of Panax notoginseng root rot can be significantly inhibited; however, with the increase of the concentration of pinolenic acid, the inhibitory effect weakens.

[0021] The lowest concentration of pinolenic acid inhibiting Cryptosporiopsis is 0.0001 ppm.

[0022] When the concentration of pinolenic acid is 0.0001 ppm, the mycelial growth of the rust rot fungus is significantly lower than that of the control group, indicating that when the concentration of pinolenic acid is low, it can significantly inhibit the occurrence of root rot in Panax notoginseng. As the concentration of pinolenic acid increases, the inhibitory effect slightly increases, but there is no significant difference within the experimental concentration range of 0.0001 - 0.1 ppm.

[0023] When the concentration of pinolenic acid is greater than 0.0001 ppm, it can slightly increase the growth of the beneficial fungus Trichoderma. Within the experimental concentration range of 0.001 - 0.1 ppm, it can increase the growth of Trichoderma, and the growth promotion effect is the best at 0.1 ppm.

[0024] When the concentration of pinolenic acid is greater than 0.001 ppm, it can increase the growth of the beneficial fungus Penicillium. As the concentration increases, the growth promotion effect becomes more obvious. Within the experimental concentration range of 0.001 - 0.1 ppm, it can increase the growth of Penicillium.

[0025] When the concentration of pinolenic acid is greater than 0.001 ppm, it can increase the growth of the beneficial fungus Mortierella. As the concentration increases, the growth promotion effect becomes more obvious. Within the experimental concentration range of 0.001 - 0.1 ppm, it can increase the growth of Mortierella.

[0026] The present invention provides the application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in increasing the dry weight of single above-ground plant of Panax notoginseng at a concentration of 0.01 ppm;

[0027] The present invention provides the application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in increasing the dry weight of single underground plant of Panax notoginseng at a concentration of 0.01 ppm.

[0028] Pinolenic acid or its derivatives or its pharmaceutically acceptable salts of the present invention can increase the root length of the fibrous roots of Panax notoginseng at 0.01 ppm.

[0029] Pinolenic acid or its derivatives or its pharmaceutically acceptable salts of the present invention can increase the root surface area of the fibrous roots of Panax notoginseng at 0.01 ppm.

[0030] Pinolenic acid or its derivatives or its pharmaceutically acceptable salts of the present invention can increase the root volume of the fibrous roots of Panax notoginseng at 0.01 ppm.

[0031] The beneficial effects of the present invention:

[0032] 1) Pinolenic acid or its derivatives or its pharmaceutically acceptable salts described in the present invention are of great significance in preventing and treating root rot caused by Fusarium oxysporum and the rust rot fungus of Panax notoginseng. In particular, they can inhibit the growth of Fusarium oxysporum and the rust rot fungus of Panax notoginseng, thereby effectively preventing and treating root rot of Panax notoginseng, and providing a new control agent for preventing and controlling diseases of Panax notoginseng.

[0033] 2) Pinolenic acid can increase the dry weight per plant of the above-ground and underground parts of Panax notoginseng, promote the growth of the fibrous roots of Panax notoginseng, meet the requirements of sustainable development, and has broad prospects for research and market application.

[0034] 3) The present invention provides an application of pinolenic acid, a metabolite secreted by pine roots, in Panax notoginseng, which can not only prevent and control the root rot of Panax notoginseng, but also increase the dry weight of Panax notoginseng. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0036] Figure 1 It shows the inhibitory effect of pinolenic acid on Fusarium oxysporum provided in Example 1 of the present invention.

[0037] Figure 2 It is a photo of the drug-containing plate of pinolenic acid on Fusarium oxysporum provided in Example 1 of the present invention.

[0038] Figure 3 It shows the growth-promoting effect of pinolenic acid on Colletotrichum gloeosporioides provided in Example 2 of the present invention.

[0039] Figure 4 It is a photo of the drug-containing plate of pinolenic acid on Colletotrichum gloeosporioides provided in Example 2 of the present invention.

[0040] Figure 5 It shows the growth-promoting effect of pinolenic acid on Trichoderma viride provided in Example 3 of the present invention.

[0041] Figure 6 It is a photo of the drug-containing plate of pinolenic acid on Trichoderma viride provided in Example 3 of the present invention.

[0042] Figure 7 It shows the growth-promoting effect of pinolenic acid on Penicillium provided in Example 4 of the present invention.

[0043] Figure 8 It shows the growth-promoting effect of pinolenic acid on Mortierella provided in Example 5 of the present invention.

[0044] Figure 9 It is a photo of the drug-containing plate of pinolenic acid on Mortierella provided in Example 5 of the present invention.

[0045] Figure 10 It shows the effect of pinolenic acid on the above-ground dry weight per plant of Panax notoginseng provided in Example 6 of the present invention.

[0046] Figure 11Effect diagram of pinolenic acid on the underground dry weight of Panax notoginseng potted plants provided in Example 6 of the present invention.

[0047] Figure 12 Effect diagram of pinolenic acid on the root length of fibrous roots of Panax notoginseng provided in Example 6 of the present invention.

[0048] Figure 13 Effect diagram of pinolenic acid on the root surface area of fibrous roots of Panax notoginseng provided in Example 6 of the present invention.

[0049] Figure 14 Effect diagram of pinolenic acid on the root volume of fibrous roots of Panax notoginseng provided in Example 6 of the present invention.

[0050] Figure 15 Drug-containing plate of palmitic acid against Fusarium oxysporum provided in Comparative Example 1 of the present invention.

[0051] Figure 16 Inhibitory effect diagram of palmitic acid on Fusarium oxysporum provided in Comparative Example 1 of the present invention.

[0052] Figure 17 Effect of palmitic acid on the survival rate of Panax notoginseng provided in Comparative Example 2 of the present invention.

[0053] Figure 18 Effect of palmitic acid on the above-ground dry weight per plant of Panax notoginseng provided in Comparative Example 2 of the present invention.

[0054] Figure 19 Effect of palmitic acid on the underground dry weight per plant of Panax notoginseng provided in Comparative Example 2 of the present invention.

[0055] In the figure, CK is the control group. Detailed implementation manners

[0056] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings in the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention without making creative efforts shall fall within the protection scope of the present invention.

[0057] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0058] Example 1

[0059] This example verified the inhibitory effect of pinolenic acid on Fusarium. The specific experimental steps were as follows:

[0060] 1. Put 200 ml of PDA medium (40 g of potato; 3 g of agar; 4 g of glucose; 200 ml of water) into a high-pressure steam sterilizer and sterilize for 15 min at 121 °C. After sterilization, place it in a laminar flow hood and sterilize with ultraviolet light for 20 min.

[0061] 2. Prepare the compound solution: Dissolve 1 mg of pinolenic acid in 1 ml of methanol solution to prepare stock solution ①.

[0062] Take 100 μL of ① and add 900 μL of methanol → ②.

[0063] Take 100 μL of ② and add 900 μL of methanol → ③.

[0064] Take 100 μL of ③ and add 900 μL of methanol → ④.

[0065] The control is methanol → ⑤.

[0066] 3. After sterilization, use a pipette to aspirate 400 μL each of ④ - ① and add them to 400 ml of the medium to obtain media with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm respectively. For the control group, take 1 ml of methanol solution and add it to 99 ml of the medium. Shake well and pour into plates, then let it cool. Use a punch with a diameter of 5 mm to punch the Fusarium into a mycelial disc of about 5 mm in size, and use an inoculation needle to inoculate the mycelial disc into the medium containing the compound solution. Place it in an incubator and culture at 26 - 28 °C for 5 d. After 5 d, observe the colony morphology and measure the colony diameter.

[0067] As Figure 1 、 Figure 2 shown, the results indicate that when the concentration of pinolenic acid is 0.001 ppm, the growth of Fusarium is significantly inhibited; as the concentration increases, the antibacterial effect weakens. Therefore, the optimal concentration of pinolenic acid is 0.001 ppm.

[0068] Example 2

[0069] This example verifies the inhibitory effect of pinolenic acid on Cylindrocarpon destructans. The specific experimental steps are as follows:

[0070] 1. Put 200 ml of PDA medium (40 g of potato; 3 g of agar; 4 g of glucose; 200 ml of water) into a high-pressure steam sterilizer and sterilize for 15 min at 121 °C. After sterilization, place it in a laminar flow hood and sterilize with ultraviolet light for 20 min.

[0071] 2. Prepare the compound solution: Dissolve 10 mg of pinolenic acid in 10 ml of methanol solution to prepare stock solution ①.

[0072] Take 100 μL of ① and add 900 μL of methanol → ②.

[0073] Take 100 μL ② and add 900 μL of methanol → ③

[0074] Take 100 μL ③ and add 900 μL of methanol → ④

[0075] The control is methanol → ⑤

[0076] 3. After sterilization, use a pipette to aspirate 400 μL each of ④ - ① and add them to 400 ml of the culture medium to obtain culture media with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm respectively. For the control group, take 1 ml of the methanol solution and add it to 99 ml of the culture medium. Shake well and pour into plates, then cool. Use a punch with a diameter of 5 mm to punch the rust rot fungus into fungal cakes of about 5 mm in size, and use an inoculation needle to inoculate the fungal cakes into the culture medium containing the compound solution. Place it in an incubator and culture at 26 - 28 °C for 7 days. After 7 days, observe the colony morphology and measure the colony diameter.

[0077] As Figure 3 、 Figure 4 shown, the results indicate that when the concentration of pinolenic acid is 0.0001 ppm, the growth of the rust rot fungus is significantly inhibited; as the concentration increases, the antibacterial effect gradually weakens. Therefore, the application range of pinolenic acid is 0.0001 ppm - 0.1 ppm.

[0078] Example 3

[0079] This example verified the growth - promoting effect of pinolenic acid on Trichoderma through a bottle - cultivation experiment. The specific experimental steps are as follows:

[0080] 1. Prepare the compound into a solution: Take 10 mg of pinolenic acid and dissolve it in 10 ml of methanol solution to prepare the stock solution ①

[0081] Take 100 μL ① and add 900 μL of methanol → ②

[0082] Take 100 μL ② and add 900 μL of methanol → ③

[0083] Take 100 μL ③ and add 900 μL of methanol → ④

[0084] The control is methanol → ⑤

[0085] 2. After sterilization, use a pipette to aspirate 400 μL each of ④ - ① and add them to 400 ml of the culture medium to obtain culture media with concentrations of 0.0001 ppm, 0.00, 0.01 ppm, and 0.1 ppm respectively. For the control group, take 1 ml of the methanol solution and add it to 99 ml of the culture medium. Shake well and pour into plates, then cool. Use a punch with a diameter of 5 mm to punch the rust rot fungus into fungal cakes of about 5 mm in size, and use an inoculation needle to inoculate the fungal cakes into the culture medium containing the compound solution. Place it in an incubator and culture at 26 - 28 °C for 2 days. After 2 days, observe the colony morphology and measure the colony diameter.

[0086] As Figure 5 , Figure 6 shown, the results indicate that when the concentration of pinolenic acid is 0.1 ppm, the colony diameter of Trichoderma is significantly higher than that of the control group, indicating that pinolenic acid has a significant growth-promoting effect on Trichoderma. When the concentration of pinolenic acid continues to increase, the promotion effect does not increase significantly. Therefore, the application range of pinolenic acid is 0.0001 ppm - 0.1 ppm, and the effect is the best at high concentrations.

[0087] Example 4

[0088] This example verified the growth-promoting effect of pinolenic acid on Penicillium through a bottle cultivation experiment. The specific experimental steps are as follows:

[0089] 1. Prepare the compound solution: Dissolve 10 mg of pinolenic acid in 10 ml of methanol solution to prepare stock solution ①.

[0090] Take 100 μL of ① and add 900 μL of methanol → ②

[0091] Take 100 μL of ② and add 900 μL of methanol → ③

[0092] Take 100 μL of ③ and add 900 μL of methanol → ④

[0093] The control is methanol → ⑤

[0094] 2. After sterilization, use a pipette to aspirate 400 μL of ④ - ① each and add them to 400 ml of the culture medium to obtain culture media with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm respectively. For the control group, take 1 ml of methanol solution and add it to 99 ml of the culture medium. Shake well and pour into plates, then cool. Dilute the cultured Penicillium with sterile water until the concentration of the spore suspension is 10 4 CFU / mL. Use a pipette to aspirate 50 μL and add it to the plate, and use a spreader to evenly spread the bacterial solution on the plate until there is no water on the plate surface. Then place the plate in an incubator and culture at 26 - 28 °C for 2 d. After 2 d, observe the colony morphology and count the number of colonies.

[0095] As Figure 7 shown, the results indicate that when the concentration of pinolenic acid is 0.001 ppm, the number of Penicillium is significantly higher than that of the control group, indicating that pinolenic acid has a significant growth-promoting effect on Penicillium. And as the concentration increases, the growth-promoting effect gradually increases. Therefore, the application range of pinolenic acid is 0.001 ppm - 0.1 ppm.

[0096] Example 5

[0097] This example verified the growth-promoting effect of pinolenic acid on Mortierella through a bottle cultivation experiment. The specific experimental steps are as follows:

[0098] 1. Prepare the compound into a solution: Take 10 mg of pinolenic acid and dissolve it in 10 ml of methanol solution to prepare mother liquor ①.

[0099] Take 100 μL of ① and add 900 μL of methanol → ②

[0100] Take 100 μL of ② and add 900 μL of methanol → ③

[0101] Take 100 μL of ③ and add 900 μL of methanol → ④

[0102] The control is methanol → ⑤

[0103] 2. After sterilization, use a pipette to aspirate 400 μL each of ④ - ① and add them to 400 ml of culture medium to obtain culture media with concentrations of 0.0001 ppm, 0.001 ppm, 0.01 ppm, and 0.1 ppm respectively. For the control group, take 1 ml of methanol solution and add it to 99 ml of culture medium. Shake well and pour into plates, then cool. Use a punch with a diameter of 5 mm to punch the Mortierella into fungal cakes about 5 mm in size, and use an inoculation needle to inoculate the fungal cakes into the culture medium containing the compound solution. Place it in an incubator and culture at 26 - 28 °C for ⑦ days. After 7 days, observe the colony morphology and measure the colony diameter.

[0104] As Figure 8 、 Figure 9 shown, the results indicate that when the concentration of pinolenic acid is 0.001 ppm, the colony diameter of Mortierella is significantly higher than that of the control group, indicating that pinolenic acid has a significant growth - promoting effect on Mortierella; and as the concentration increases, the growth - promoting effect gradually increases. Therefore, the application range of pinolenic acid is 0.001 ppm - 0.1 ppm, and the effect is the best at low concentrations.

[0105] Example 6

[0106] This example verifies the effect of pinolenic acid on improving the dry weight per plant and root growth of annual Panax notoginseng. The specific experimental steps are as follows:

[0107] 1. Prepare the compound into a solution: Considering the plate effect, select a concentration of 0.01 ppm of pinolenic acid for the Panax notoginseng pot experiment. Take 0.1 mg of pinolenic acid and add it to 1 L of sterile water to make the mother liquor.

[0108] 2. Take 10 ml of the mother liquor and add it to 1 L of water to obtain a concentration of 0.01 ppm. After mixing, the compound is applied at a concentration of 50 ml per pot. For the control, take 1 ml of methanol and add it to 1 L of water, and then perform root irrigation experiments once a week for 6 weeks. After the Panax notoginseng continues to grow for two months, sample the potted Panax notoginseng, wash it, count the number of Panax notoginseng plants treated with each concentration gradient of the compound, scan the fibrous roots of Panax notoginseng, put the above-ground and underground parts into envelope bags respectively, blanch at 115 °C for 30 minutes, dry at 60 °C for 3 days, weigh the dry weight, and calculate the dry weight.

[0109] Figure 10 and Figure The results showed that root application of pinolenic acid at a concentration of 0.01 ppm could significantly increase the dry weight per plant of the above-ground and underground parts. ​ 、 ​ and ​ Root application of pinolenic acid at a concentration of 0.01 ppm could significantly increase the growth of the fibrous roots of Panax notoginseng. The comprehensive results showed that it could significantly improve the growth of Panax notoginseng plants at 0.01 ppm.

[0110] Comparative Example 1

[0111] This comparative example provided another substance detected in root exudates - palmitic acid (Plamitic acid, CAS: 10025-99-7), and tested its inhibitory effect on Colletotrichum dematium. The specific experimental steps were only different from those in Example 1 in that pinolenic acid was replaced with palmitic acid.

[0112] The results were as ​ 、 ​ shown. After adding palmitic acid, the diameter of the fungal cake on the plate was significantly higher than that of the control at concentrations above 0.01 ppm. The results showed that palmitic acid promoted the growth of Colletotrichum dematium at concentrations of 0.01 ppm and above.

[0113] Comparative Example 2

[0114] This comparative example verified the effect of another substance detected in root exudates - palmitic acid (Plamitic acid, CAS: 10025-99-7) on the growth of potted Panax notoginseng through a bottle cultivation experiment.

[0115] The results were as ​ 、 ​ and ​ shown. The results showed that m-chlorobenzoic acid could not increase the dry weight of the above-ground part and the growth of fibrous roots of Panax notoginseng.

[0116] In addition to the compounds exemplified in the above comparative examples, the present invention has also verified a number of other compounds obtained from the root exudates of pine trees, and found that many other compounds cannot inhibit Fusarium and / or Cryptodiaporthe, nor promote the growth of Panax notoginseng, and will not be listed one by one here.

[0117] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant pathogen bacteriostatic agent, characterized in that The plant pathogen bacteriostatic agent contains pinolenic acid or its derivatives or its pharmaceutically acceptable salts.

2. The bacteriostatic agent according to claim 1, wherein The plant pathogen is Fusarium and / or Coriolus versicolor.

3. Application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in preventing and treating Panax notoginseng root rot.

4. The application according to claim 3, characterized in that, The pinolenic acid or its derivatives or its pharmaceutically acceptable salts inhibit the plant pathogens Fusarium and / or Coriolus versicolor, and at the same time increase the growth of beneficial fungi Trichoderma, Penicillium and / or Mortierella.

5. Application of pinolenic acid or its derivatives or its pharmaceutically acceptable salts in promoting the growth of Panax notoginseng.

6. The application according to claim 5, wherein The pinolenic acid or its derivatives or its pharmaceutically acceptable salts increase the dry weight or fresh weight of Panax notoginseng.

7. The application according to claim 6, characterized in that, The pinolenic acid or its derivatives or its pharmaceutically acceptable salts increase the dry weight or fresh weight of the roots and / or leaves of Panax notoginseng.

8. The application according to claim 5, characterized in that, The pinolenic acid or its derivatives or its pharmaceutically acceptable salts increase the dry weight per above-ground single plant of Panax notoginseng, increase the dry weight per below-ground single plant of Panax notoginseng, increase the root length of the fibrous roots of Panax notoginseng, increase the root surface area of the fibrous roots of Panax notoginseng and / or increase the root volume of the fibrous roots of Panax notoginseng.

9. The application according to any one of claims 3 to 8, characterized in that The use concentration of the pinolenic acid or its derivatives or its pharmaceutically acceptable salts is not less than 0.0001 ppm.

10. The application according to claim 9, characterized in that: The use concentration of the pinolenic acid or its derivatives or its pharmaceutically acceptable salts is 0.0001 ppm - 0.1 ppm.

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