A double tube cleansing product with exfoliation and blackhead removal and a preparation method and application thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a double-tube cleansing product with the functions of removing keratin and blackheads, and a preparation method and application thereof. Specifically, the present application applies a keratin and blackhead removing composition, a surfactant, a preservative, a humectant, a thickening agent, and a fatty acid to a cleansing product, and adopts a double-tube form, wherein the cleansing cream is the inner tube component, and the outer tube is mainly a gel system. The effective components in the blackhead removing composition include papaya fruit extract, fig ferment liquor, and yeast / zinc fermentation product. The adoption of the double-tube form can avoid the inactivation of enzyme components caused by long-time contact with surfactant components, thereby prolonging the shelf life of the product, and realizing the functions of mild keratin removal and blackhead removal. Therefore, the present application has good practical application value.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a facial cleanser with dual functions of exfoliation and blackhead removal, its preparation method, and its application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] The stratum corneum, the final product of epidermal keratinocyte differentiation, is located on the outermost layer of the skin. It acts as the skin's first line of defense against external stimuli, serving as a barrier to protect the body from harsh environmental factors, limit moisture loss, and prevent the entry of external microorganisms or compounds. If keratinocyte metabolism is too slow, keratinocytes will accumulate excessively, causing the stratum corneum to thicken. An excessively thick stratum corneum not only inhibits the generation of new keratinocytes but also affects the skin's absorption of moisture and skincare products, leading to dry, dull, rough, and hardened skin, and even exacerbating skin problems such as acne and keratosis pilaris. It is necessary to properly manage the skin's keratinocyte metabolism process. On the one hand, this helps remove excess keratin, making the skin smoother, more delicate, and radiant; on the other hand, it avoids over-exfoliation that damages the skin barrier. Therefore, exfoliating products have become a focus of attention in the cosmetics and skin health industries. The inventors discovered that common ingredients, such as salicylic acid and fruit acids, have good exfoliating effects, but their low pH value makes them somewhat irritating. Improper use can easily lead to over-exfoliation and damage to the skin barrier.

[0004] Furthermore, blackheads, also known as open comedones, are generally considered to be plug-like substances composed of sebum, cell debris, and bacteria. Their formation process involves excessive oil buildup in pores, enlarging them as the oil is squeezed. The oil then becomes a whitehead, which breaks through the skin and oxidizes upon contact with air, turning black and potentially developing into acne. Currently, there are numerous blackhead removal products on the market, with varying effectiveness. The inventor discovered that traditional blackhead removal products primarily use strong acidic systems such as azelaic acid, resulting in highly irritating products.

[0005] Cleansing is the first step in skincare; skincare begins with washing your face. Therefore, there is an urgent need to develop a gentle yet effective cleansing product that removes blackheads and exfoliates to improve skin problems such as thickened stratum corneum and blackheads, allowing consumers to achieve skincare results while washing their face. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the present invention aims to provide a dual-tube facial cleanser for exfoliation and blackhead removal, along with its preparation method and application. Specifically, the present invention incorporates an exfoliating blackhead removal composition, surfactants, preservatives, moisturizers, thickeners, and fatty acids into the facial cleanser. It employs a dual-tube design: the inner tube contains the cleansing milk, while the outer tube primarily contains a gel system. The blackhead removal composition contains papaya fruit extract, fig ferment broth, and yeast / zinc fermentation products as active ingredients. This dual-tube design prevents the enzymes from prolonged contact with the surfactants, thus extending the product's shelf life and achieving both gentle exfoliation and blackhead removal. Based on the above research findings, the present invention is thus completed.

[0007] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0008] In a first aspect, the present invention provides a facial cleansing product with dual functions of exfoliation and blackhead removal, the facial cleansing product comprising an outer tube and an inner tube.

[0009] The outer tube material, by weight percentage, comprises 3-8% exfoliating blackhead composition, 5-15% moisturizer, 2-8% thickener, 0.5-1.5% preservative, 0.1-0.5% pH adjuster, and the balance being water. Further, the exfoliating blackhead composition consists of papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product; the mass ratio of the papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product is 1-10:1-10:1-10. Further, the outer tube material is a gelling agent.

[0010] The inner tube material, by mass percentage, comprises 35-55% surfactant, 2-5% thickener, 10-15% humectant, 0.5-1.0% preservative, and 0.5-3% fatty acid, with the balance being water; furthermore, the surfactant comprises amino acid-type surfactant and non-amino acid-type surfactant; the mass ratio of the amino acid-type surfactant to the non-amino acid-type surfactant is 3-6:4-5. The inner tube material is formulated as a paste emulsion.

[0011] Furthermore, the inner tube material also contains sodium citrate at a content of 2-4%. Through research, this invention unexpectedly discovered that the addition of sodium citrate is beneficial for improving the stability of the inner tube material paste. In particular, the sodium citrate can coexist with lauric acid, increasing the liquid crystal structure of the inner tube cleaning fabric paste, thereby improving the stability of the inner tube material paste.

[0012] A second aspect of the present invention provides a method for preparing the above-mentioned dual-tube facial cleanser that removes exfoliation and blackheads, the method comprising preparing an outer tube gel formulation and an inner tube cleansing milk formulation.

[0013] The preparation method further includes filling the outer tube gel formulation and the inner tube facial cleanser formulation into a container. In use, the container tube is squeezed to dispense the contents from both the inner and outer tubes, which are then mixed thoroughly before use.

[0014] A third aspect of the present invention provides the use of the above-described product in the preparation of exfoliating and blackhead-removing cosmetics.

[0015] In a fourth aspect, the present invention provides an exfoliating and blackhead-removing cosmetic product, the cosmetic product comprising the above-mentioned product.

[0016] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0017] 1. The above technical solution provides a blackhead-removing and exfoliating composition that is widely available, easy to use, and has significant exfoliating and blackhead-removing effects. It meets the blackhead and exfoliation needs of oily skin and has a very large market prospect.

[0018] 2. In the above technical solution, the inner tube of the facial cleansing emulsion adopts a liquid crystal structure facial cleansing system. The liquid crystal structure system can combine lipophilic ingredients, has good biocompatibility, and can better achieve the functions of cleansing and makeup removal.

[0019] 3. The above technical solution adopts a dual-tube design, which can retain the activity of the blackhead removal and exfoliation composition for a long time and maintain the skin care function of the product for a long time, thus having good practical application value. Attached Figure Description

[0020] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0021] Figure 1 The following is an extrusion effect diagram of the product in Example 4 (the inner tube of the facial cleanser tube contains a white semi-transparent paste, and the outer tube of the gel tube contains a transparent gel). A is a product appearance diagram, B is a product outlet diagram, and C is a product extrusion diagram.

[0022] Figure 2 This is a liquid crystal structure diagram of Example 9. Detailed Implementation

[0023] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0024] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0025] As mentioned earlier, existing blackhead removal and exfoliation products generally suffer from poor removal effects, high irritation, and damage to the skin barrier. Therefore, there is an urgent need to develop a gentle yet effective cleansing product for removing blackheads and exfoliating to improve skin problems such as thickened stratum corneum and blackheads.

[0026] Liquid crystals are a physical state between solids and liquids, retaining the fluidity and continuity of liquids while exhibiting the anisotropy of solid crystals. They are thermodynamically stable phases between solids and liquids. In cosmetics, liquid crystals are often referred to as lyotropic liquid crystals. Amphiphilic lipids are arranged in layers, with hydrophilic groups arranged orderly on the outer surface of the lipid layers and hydrophobic groups located on the inner surface. The layers are separated by interlayer bound water. The laminar liquid crystal contains a continuous phase of both hydrophilic and hydrophobic components, allowing it to encapsulate hydrophilic and lipophilic substances, thus facilitating the effectiveness of the ingredients. The structure of laminar liquid crystals is similar to the layered structure of the phospholipid bilayer in the extracellular lipids of skin keratinocytes, and the layers are filled with water channels, facilitating sliding and providing a skin-adhering feel and excellent application and spreading effect.

[0027] In view of this, in a typical embodiment of the present invention, a facial cleansing product with dual functions of exfoliation and blackhead removal is provided, the facial cleansing product being composed of an outer tube and an inner tube.

[0028] The outer tube material, by weight percentage, comprises 3-8% exfoliating blackhead composition, 5-15% moisturizer, 2-8% thickener, 0.5-1.5% preservative, 0.1-0.5% pH adjuster, and the balance being water. Further, the exfoliating blackhead composition consists of papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product; the mass ratio of the papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product is 1-10:1-10:1-10. Further, the outer tube material is a gelling agent.

[0029] The inner tube material, by weight percentage, comprises 35-55% surfactant, 2-5% thickener, 10-15% humectant, 0.5-1.0% preservative, 0.5-3% fatty acid, and the balance being water; furthermore, the surfactant comprises amino acid-type surfactant and non-amino acid-type surfactant; the mass ratio of the amino acid-type surfactant to the non-amino acid-type surfactant is 3-6:4-5. The inner tube material forms a cream emulsion, which has a well-stable liquid crystal structure cleansing system, can bind lipophilic components, has good biocompatibility, and can achieve good cleansing and makeup removal effects.

[0030] In another specific embodiment of the present invention, the papaya fruit extract in the outer tube material can be obtained commercially. In one specific embodiment of the present invention, the commercial name of the papaya fruit extract is PAPAYAHYDROGLYCOLIC EXTRACT BG, and its full composition is: water, butylene glycol, papaya fruit extract, 1,2-hexanediol, purchased from Guangzhou Bochi Biotechnology Co., Ltd., wherein the active ingredient in the papaya fruit extract is papain.

[0031] The fig fermentation broth can be obtained commercially or prepared at home. In one specific embodiment of the present invention, the fig fermentation broth is prepared by the following method:

[0032] Lactic acid bacteria are inoculated into a liquid fermentation medium containing fig powder for fermentation treatment, and then filtered to remove bacteria and impurities to obtain the product.

[0033] Specifically, the lactic acid bacteria can be Lactobacillus rhamnosus; by using the above-mentioned lactic acid bacteria for fermentation treatment, the fermentation product has better activity effects such as removing blackheads; the inoculation amount is controlled at 1-5% (V / V), preferably 3%;

[0034] The fig powder can be obtained by drying and pulverizing figs and then passing them through a 40-80 mesh (preferably 60 mesh) sieve; the amount of fig powder added is controlled at 1-10% (w / v, g / mL), preferably 5%;

[0035] The liquid fermentation medium also contains a carbon source, which is more conducive to the fermentation culture of lactic acid bacteria. Preferably, the carbon source is white sugar, and the mass-volume fraction of white sugar in the liquid fermentation medium is 1-6%, preferably 4% (w / v, g / mL).

[0036] The specific conditions for the fermentation treatment are: fermentation culture at 25-40℃ (preferably 37℃) for 24-96 hours, preferably 72 hours.

[0037] The filtration can be performed using a ceramic membrane with a pore size of 50-200 nm, thereby achieving effective sterilization and impurity removal.

[0038] The yeast / zinc fermentation product can be obtained commercially. In one specific embodiment of the present invention, the yeast / zinc fermentation product is commercially known as Yeast Essence Z20(N), and its full composition is: yeast / zinc fermentation product, 1,2-pentanediol, and ethylhexylglycerin, purchased from Angel Yeast Co., Ltd.

[0039] The moisturizer is any one or more of glycerin, 1,2-hexanediol, dipropylene glycol, methyl propylene glycol, butylene glycol, ethylhexylglycerin, sodium hyaluronate, or hyaluronic acid derivatives.

[0040] The thickener is any one or more of the following: sodium polyacrylate, carbomer, xanthan gum, hydroxyethyl cellulose, polyacrylate crosspolymer 6, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, magnesium aluminum silicate, hydroxypropyl starch phosphate, acrylate (type) copolymer, PEG 120 methyl glucoside dioleate, and PEG 150 distearate.

[0041] The preservative is any one or more of p-hydroxyacetophenone, octanoyl hydroxamic acid, phenoxyethanol, and chlorphenesin;

[0042] The pH adjuster is any one or more of tromethamine, triethanolamine, and arginine;

[0043] In another specific embodiment of the present invention, the amino acid-type surfactant in the inner tube material is any one or more of the following: potassium cocoyl glycinate, potassium lauroyl glycinate, TEA salt of cocoyl glycinate, sodium cocoyl glycinate, sodium lauroyl glycinate, sodium cocoyl glutamate, TEA salt of cocoyl glutamate, sodium lauroyl sarcosinate, disodium cocoyl glutamate, and sodium cocoyl aminopropionate.

[0044] The non-amino acid surfactant is any one or more of sodium lauroyl taurate, sodium methyl cocoyl taurate, potassium cocoyl hydrolyzed collagen, cocamidopropyl hydroxysulfonate, and sodium lauroyl amphoteric acetate.

[0045] The fatty acid is any one or more of lauric acid, myristic acid, stearic acid, and palmitic acid; wherein, lauric acid is preferred.

[0046] The thickener is any one or more of the following: modified corn starch, hydroxypropyl starch phosphate, PEG-120 methyl glucoside, and sodium acrylate / sodium acryloyl dimethyl taurate copolymer.

[0047] The moisturizer is any one or two of glycerin, ethylhexylglycerin and 1,2-hexanediol;

[0048] The preservative is any one or more of phenoxyethanol, p-hydroxyacetophenone, and octanoyl hydroxamic acid.

[0049] In another specific embodiment of the present invention, the inner tube material further comprises sodium citrate at a content of 2-4%. The present invention unexpectedly discovered through research that the addition of sodium citrate is beneficial to improving the stability of the inner tube material paste. In particular, the sodium citrate can coexist with lauric acid, increasing the liquid crystal structure of the inner tube cleaning fabric paste, thereby improving the stability of the inner tube material paste.

[0050] In another specific embodiment of the present invention, a method for preparing the above-mentioned dual-tube facial cleanser with exfoliation and blackhead removal is provided, wherein the preparation method includes preparing an outer tube gel formulation and an inner tube facial cleanser milk formulation;

[0051] The preparation method of the outer tube gel formulation includes:

[0052] S1-1: Mix water, thickener, and humectant, stir, heat to 80-85℃, keep warm and stir until completely dissolved, then cool down.

[0053] S1-2: When the temperature drops to 45-50℃, add the pH adjuster and stir to mix and dissolve evenly;

[0054] S1-3: When the temperature drops to 40-45℃, add the blackhead removal and exfoliating composition and preservative, stir and mix until evenly dissolved, then discharge.

[0055] The preparation method of the facial cleanser formulation in the inner tube includes:

[0056] S2-1: Mix surfactant, water, humectant, fatty acid, thickener, and sodium citrate, stir and heat to 90-95℃, keep warm and stir until completely dissolved, keep warm for 30-40 minutes and then cool down.

[0057] S2-2: When the temperature drops to 45-50℃, add humectant and preservative, disperse / dissolve evenly, keep warm and stir for 10-15 minutes, and then discharge.

[0058] Furthermore, the preparation method also includes filling the outer tube gel formulation and the inner tube facial cleanser formulation into a container, with a filling mass ratio of outer tube gel to inner tube facial cleanser of 0.5-5:1 (preferably 1:1); during use, the container tube is squeezed, and the inner and outer tubes are dispensed separately (the dispensing mass ratio can be 1:1), and then mixed evenly before use. It should be noted that, for ease of use, the above technical solution prepares the outer tube gel formulation and the inner tube facial cleanser formulation separately using outer and inner tubes. However, it is clear that as long as the two are stored in different containers before formal use to achieve isolation, the desired effect can be achieved. The resulting packaging methods and corresponding products also fall within the scope of protection of this application.

[0059] In another specific embodiment of the present invention, the above-mentioned product is provided for use in the preparation of exfoliating and blackhead-removing cosmetics.

[0060] In another specific embodiment of the present invention, an exfoliating and blackhead-removing cosmetic is provided, the cosmetic comprising the above-mentioned product. Tests have proven that the cosmetic prepared by the present invention has significant exfoliating and blackhead-removing effects, good biocompatibility, and better achieves the functions of cleansing and makeup removal. Furthermore, the dual-tube design avoids the inactivation of enzyme-active ingredients due to contact with surfactants, thereby effectively extending the product's shelf life.

[0061] The present invention will be further described below with reference to embodiments. The present invention is further illustrated by means of embodiments, but this does not limit the present invention to the scope of the embodiments described. Based on the embodiments of the present invention, any changes to the present invention by those skilled in the art without inventive step are within the protection scope of the present invention. Furthermore, in the embodiments of the present invention, unless otherwise specified, all raw materials used in the preparation are commercially available products well known to those skilled in the art.

[0062] Examples 1-3 and Comparative Examples 1-6

[0063] The specific plan is shown in Table 1 below, calculated by mass parts. The preparation method of the exfoliating and blackhead removal composition is as follows: Papaya fruit extract, yeast / zinc fermentation product, and fig fermentation liquid are mixed together and dissolved in purified water.

[0064] Table 1. List of components for specific embodiments and comparative examples.

[0065] Papaya fruit extract Yeast / Zinc Fermentation Products Fig fermentation liquid Purified water Example 1 1.0 copy 1.0 copy 1.0 copy TO 100 Example 2 5.0 copies 5.0 copies 5.0 copies TO 100 Example 3 10.0 copies 10.0 copies 10.0 copies TO 100 Comparative Example 1 5.0 copies —— —— TO 100 Comparative Example 2 —— 5.0 copies —— TO 100 Comparative Example 3 —— —— 5.0 copies TO 100 Comparative Example 4 5.0 copies 5.0 copies —— TO 100 Comparative Example 5 —— 5.0 copies 5.0 copies TO 100 Comparative Example 6 5.0 copies —— 5.0 copies TO 100

[0066] The papaya fruit extract in the above-mentioned blackhead-removing and exfoliating composition is marketed as PAPAYA HYDROGLYCOLICEXTRACT BG, and its full composition consists of water, butylene glycol, papaya fruit extract, and 1,2-hexanediol. It was purchased from Guangzhou Bochi Biotechnology Co., Ltd., and the active ingredient in the papaya fruit extract is papain. The yeast / zinc fermentation product is marketed as Yeast Essence Z20(N), and its full composition consists of yeast / zinc fermentation product, 1,2-pentanediol, and ethylhexylglycerin. It was purchased from Angel Yeast Co., Ltd. None of the above ingredients contain live bacteria.

[0067] Fig fermentation broth was obtained through the following method:

[0068] A suspension of *Lactobacillus rhamnosus* CGMCC No. 27781 was inoculated into MRS medium (lactobacterial medium, Maclean's) and incubated statically at 37°C for approximately 15 hours. Once the bacterial concentration (OD600) reached 2.0, it was inoculated at a rate of 2% (v / v) into a sterile fermentation medium containing water, 5% (w / v) dried, pulverized fig powder (passed through a 60-mesh sieve), and 4% (w / v) white sugar. Fermentation was carried out at 37°C for approximately 72 hours to obtain the fig fermentation product. The fermentation product was then filtered through a 200 nm pore size ceramic membrane to remove bacteria and impurities, yielding the fig fermented product.

[0069] The Lactobacillus rhamnosus strain was deposited on July 3, 2023, at the China General Microbiological Culture Collection Center (address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing), with accession number CGMCC No. 27781. This strain has been disclosed in Chinese patent CN117050923A.

[0070] The formulations of the dual-tube facial cleanser in Example 4 and Comparative Example 7.

[0071] The specific plan is shown in Table 2 below, calculated in parts by weight.

[0072] Table 2. List of components for specific embodiments and comparative examples.

[0073]

[0074] The preparation methods for Examples 5 and 6 are the same as those for Example 4, except that 8.0% of the sample from Example 1 is added to the gel tube in Example 5, and 8.0% of the sample from Example 3 is added to the gel tube in Example 6. The preparation methods for Comparative Examples 8 to 13 are the same as those for Example 4, except that 8.0% of the sample from Comparative Examples 1 to 6 is added to the gel tubes of Comparative Examples 8 to 13 respectively.

[0075] The formulations of the cleansing milk in Examples 7-10 and Comparative Examples 14-17.

[0076] Table 3. List of components for specific embodiments and comparative examples.

[0077]

[0078] Preparation method of dual-tube facial cleanser:

[0079] Inner tube facial cleanser:

[0080] S1: Add surfactant (potassium cocoyl glycinate, ... Add T40, sodium lauroyl sarcosinate, water, humectant (glycerin), fatty acid (lauric acid), sodium citrate, and thickener (hydroxypropyl starch phosphate, SIMULGEL EG), stir and heat to 90-95°C, keep warm and stir until completely dissolved, keep warm for 30-40 minutes, and then slowly cool down.

[0081] S2: When the temperature drops to 45-50℃, add other humectants (1,2-hexanediol) and preservatives (euxyl). TM After dispersing / dissolving PE9010 evenly, keep warm and stir for 10-15 minutes, then discharge.

[0082] Outer tube gel:

[0083] S1: Add water, thickener (hydroxypropyl starch phosphate, carbomer), humectant (glycerin), and chelating agent (EDTA2Na) to the container, stir and heat to 80-85°C, keep warm and stir until completely dissolved, and then start cooling.

[0084] S2: When the temperature drops to 45-50℃, add the pH adjuster (tromethamine) and stir to mix and dissolve evenly;

[0085] S3: When the temperature drops to 40-45℃, add the blackhead-removing and exfoliating composition (Example 2) and the preservative (Spectrastat). TM PHL), humectant (1,2-hexanediol), stir and mix until evenly dissolved, then discharge.

[0086] The products are packaged separately, with a filling ratio of gel in the outer tube to facial cleanser in the inner tube of 1:1. When using, squeeze the tube to dispense the gel from both the inner and outer tubes separately, with a 1:1 dispensing ratio. Mix thoroughly before use.

[0087] Table 4. Raw materials and sources involved in the examples and comparative examples.

[0088]

[0089] Experiment 1: In Vitro Exfoliation Efficacy Test

[0090] Pigskin was fixed between the supply and receiving chambers of the Franz cell diffusion cell, with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. 7.0 mL of receiving solution was added to the receiving chamber. After the excised skin tissue was taut and fixed, 1.5 mL of receiving solution (PBS) was added to the receiving chamber through a sampler. Air was expelled to ensure close contact between the dermis and the receiving solution. The working solutions for the test samples (i.e., the sample groups) were prepared according to the test protocol (Table 5).

[0091] Samples were added to the skin surface in the supply chamber. 50 μL of sample was added to the surface of the excised skin tissue (PBS buffer was added as a blank control to the control group surface). The sample was spread evenly radially from the center of the skin towards the edge, with a sample exposure area of ​​3.14 cm². 2 Each sample was tested in triplicate, maintained in a constant temperature water bath at (32±1)℃, ensuring the water bath jacket was free of air bubbles. After 24 hours of incubation, finger cots were used to gently rub the sample application area for 2 minutes. Then, 0.5 mL of washing buffer (0.1% Triton X-100) was added to the supply chamber, and the skin surface was cleaned by pipetting to remove exfoliated keratinocytes. The washing buffer was transferred to a 1.5 mL centrifuge tube for later use. Total protein content was determined using quantitative real-time analysis according to the BCA protein assay kit instructions.

[0092]

[0093] GraphPad Prism was used for plotting, and the results are expressed as Mean ± SD. t-tests were used for comparisons between groups. All statistical analyses were two-tailed. A p-value of 0.01 < p < 0.05 was considered statistically significant, and a p-value < 0.01 was considered highly statistically significant. Compared to groups BC, significance is indicated by *, p-value < 0.05 by *, and p-value < 0.01 by **.

[0094] Table 5 Test Plan

[0095]

[0096] Table 6 Summary of Keratinocyte Detection Results

[0097]

[0098]

[0099] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the negative control; * indicates p < 0.01 compared to the negative control.) <p<0.05。)

[0100] Table 6 shows that compared with group BC, the keratinocyte exfoliation rate of group PC was significantly increased, indicating the effectiveness of this positive control test. The exfoliation ability of the single yeast / zinc ferment was weak and not significant. Examples 1-3 and Comparative Examples 1 and 3-6 all showed significant exfoliation ability, indicating a certain promoting effect among the three. Example 4 (freshly prepared) and Comparative Example 7 (freshly prepared) both showed strong exfoliation ability, exceeding that of salicylic acid. After one month of storage, Example 4 showed a slight decrease compared to the freshly prepared version, but this was not significant. However, after one month of storage, Comparative Example 7 showed a significant decrease in exfoliation ability compared to the freshly prepared Comparative Example 7. This indicates that prolonged coexistence of the blackhead-removing exfoliating composition and surfactants can affect its function. This invention uses a dual-tube design to fully preserve the product's efficacy.

[0101] Experiment 2: Blackhead Removal Effect Test

[0102] Testing instrument: Visia-cr testing instrument.

[0103] Inclusion criteria for the test: Individuals aged 20-38 years old, assessed as having oily skin, with blackheads and enlarged pores on both sides of the nose, and those with relatively high facial sebum secretion. The sebum content on both sides of the nose was measured using a Sebumeter, with a baseline value of 200 μg / cm² in the test area. 2 Those who meet the above requirements: 65 males and 55 females, totaling 120 people, divided into 12 groups of 10 people each.

[0104] Test method: First, the number of blackheads in the subjects before washing their faces without using the sample was measured and the initial value was recorded. Then, the subjects cleaned their faces with the dual-tube cleansers of Examples 5 and 6, Comparative Examples 8-13, and Examples 4 (freshly prepared), 4 (stored at room temperature for 1 month), 7 (freshly prepared), and 7 (stored at room temperature for 1 month), respectively, for 30 minutes (the cleansing time was controlled to be the same). Macroscopic images of the target area on the subjects' faces were taken using Visia-cr, and the number of blackheads in the target area was analyzed and the values ​​were recorded. The rate of change was calculated using SPSS statistical software.

[0105] Blackhead quantity change rate = (Blackhead quantity after sample use - Initial blackhead quantity) / Initial blackhead quantity × 100%.

[0106] The results of the test on the rate of change in the number of blackheads are shown in Table 7.

[0107] Table 7 Results of the test on the rate of change in the number of blackheads

[0108]

[0109] Note: In the table, compared with the initial number of blackheads, "-" indicates that the number of blackheads has decreased; "*" indicates that the difference is statistically significant (0.01≤P<0.05); "**" indicates that the difference is statistically significant (0.001≤P<0.01).

[0110] As shown in Table 7, Examples 4 (freshly prepared), 5, and 6, and Comparative Example 7 (freshly prepared) all exhibit significant blackhead removal effects. However, Comparative Example 7 (stored at room temperature for 1 month) shows some blackhead removal effect, but its effect is significantly weaker than that of Comparative Example 7 (freshly prepared). This further illustrates that prolonged coexistence of the blackhead-removing and exfoliating composition with surfactants can affect its function. This invention employs a dual-tube design to fully preserve the product's efficacy.

[0111] Test Example 3: Stability Test

[0112] The samples from Examples 7-10 and Comparative Examples 14-17 were placed at room temperature, -18°C, 4°C, and 40°C, respectively, and subjected to thermal cycling (-18°C, 4°C, 40°C, once every 3 days) for the corresponding time to observe their stability. The stability of the product was judged by whether phenomena such as oil release, water release, or coarsening of the material occurred. If no unstable phenomena such as oil release, water release, or coarsening of the material occurred, it was considered stable.

[0113] Table 8. Stability test results of the examples and comparative examples.

[0114]

[0115] Table 8 shows that the coexistence of lauric acid and sodium citrate in different proportions in Examples 7-10 resulted in good stability. Examples 9 and 14 demonstrate that the presence of sodium citrate is beneficial to the stability of the cleansing cream. Examples 15-17 show that myristic acid, stearic acid, and palmitic acid alone are detrimental to the stability of the cleansing cream. Therefore, lauric acid and sodium citrate are beneficial to the stability of the cleansing cream.

[0116] Experiment 4: Observation of the stability of liquid crystal structures

[0117] Microscopic morphology images of the prepared samples of Examples 7-10 and Comparative Examples 14-17 were taken under a polarizing microscope.

[0118] Table 9. Experimental Results of Liquid Crystal Structure Stability

[0119]

[0120]

[0121] Table 9 shows that the stability of the liquid crystal structure in Examples 7-10 was good. Examples 9 and 14 show that the presence of sodium citrate is beneficial to the stability of the liquid crystal structure in the paste. Examples 15-17 show that myristic acid, stearic acid, and palmitic acid do not affect the stability of the liquid crystal structure in the paste at room temperature. However, the liquid crystal structure decreased under conditions of 4°C, -18°C, 40°C, and thermal cycling, which may also be related to product stability.

[0122] Experiment 5: Foam Test Experiment

[0123] The foam was tested and evaluated using a Roche foam analyzer (model 2151). The specific testing method is as follows:

[0124] Turn on the thermostat. When the thermostat reaches a certain temperature, the temperature of the pipe jacket water bath will be stabilized at 40℃±0.5℃.

[0125] Rinse the inside of the graduated tube with distilled water until it is completely rinsed, then rinse the tube wall with the test solution until it is completely rinsed as well.

[0126] Close the stopcock of the graduated tube and use another dropper to inject 50 ml of test solution to the 50 ml mark. This test solution should be preheated to 40°C.

[0127] Fill the dropper with 200 ml of test solution, which has been preheated to 40°C.

[0128] Place the dropper on the pre-prepared tube rack, perpendicular to the cross-section of the graduated tube, so that the solution flows to the center of the graduated tube. The outlet of the dropper should be placed on the 900mm mark.

[0129] Open the stopcock of the dropper to allow the solution to flow down. When the solution in the dropper has finished flowing, immediately start the stopwatch and measure the foam height. Then record the height again after 5 minutes, 10 minutes, and 15 minutes. The foam value is expressed as foam height.

[0130] Repeat the above experiment 2-3 times. Before each experiment, the container wall must be cleaned to avoid affecting the data.

[0131] The test results are shown in Table 10.

[0132] Table 10: Statistics on Foam Height of Different Products

[0133]

[0134]

[0135] As can be seen from the results in Table 10, the foam height of Comparative Examples 15-17 was significantly lower than that of Example 9, indicating that myristic acid, stearic acid, and palmitic acid affect the foaming ability of the facial cleanser. Compared with Example 9 and Comparative Example 14, the foaming ability did not change significantly, indicating that sodium citrate does not significantly affect the foaming ability of the product.

[0136] It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the given examples, those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention as needed, without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A facial cleansing product with dual functions of exfoliation and blackhead removal, characterized in that, The facial cleansing product consists of an outer tube and an inner tube. The outer tube material, by weight percentage, comprises 3-8% exfoliating blackhead composition, 5-15% moisturizer, 2-8% thickener, 0.5-1.5% preservative, 0.1-0.5% pH adjuster, and the balance being water; the exfoliating blackhead composition consists of papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product; the mass ratio of papaya fruit extract, fig fermentation broth, and yeast / zinc fermentation product is 1-10:1-10:1-10; the active ingredient in the papaya fruit extract is papain; The inner tube material, by mass percentage, comprises 35-55% surfactant, 2-5% thickener, 10-15% humectant, 0.5-1.0% preservative, 0.5-3% fatty acid, and the balance being water; the surfactant includes amino acid-type surfactants and non-amino acid-type surfactants; the mass ratio of the amino acid-type surfactants to the non-amino acid-type surfactants is 3-6:4-5; the inner tube material also contains sodium citrate, with a content of 2-4%; the fatty acid is lauric acid; The fig fermentation broth used in the outer tube material is prepared using the following method: Lactic acid bacteria are inoculated into a liquid fermentation medium containing fig powder for fermentation treatment, and then filtered to remove bacteria and impurities to obtain the product. The lactic acid bacteria mentioned are specifically Lactobacillus rhamnosus CGMCC No. 27781; the inoculum amount is controlled at 1-5% (V / V). The fig powder is specifically obtained by drying and pulverizing figs and then passing them through a 40-80 mesh sieve; the amount of fig powder added is controlled at 1-10% (w / v, g / mL). The liquid fermentation medium also contains a carbon source, which is white sugar, and the white sugar in the liquid fermentation medium has a mass-volume fraction of 1-6% (w / v, g / mL).

2. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, The lactic acid bacteria specifically refers to Lactobacillus rhamnosus CGMCC No. 27781, with an inoculation amount controlled at 3%.

3. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, The fig powder is specifically obtained by drying and pulverizing figs and then passing them through a 60-mesh sieve; the amount of fig powder added is controlled at 5%.

4. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, The sugar content in the liquid fermentation medium is 4% by mass and volume.

5. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, The specific conditions for the fermentation treatment are: fermentation culture at 25-40℃ for 24-96 hours; The filtration process employs a ceramic membrane, with a pore size of 50-200 nm.

6. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, In the outer tube material, the humectant is any one or more of glycerin, 1,2-hexanediol, dipropylene glycol, methyl propylene glycol, butylene glycol, and sodium hyaluronate. The thickener is any one or more of the following: sodium polyacrylate, carbomer, xanthan gum, hydroxyethyl cellulose, polyacrylate crosspolymer 6, ammonium acryloyl dimethyl taurate / VP copolymer, hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, magnesium aluminum silicate, hydroxypropyl starch phosphate, acrylate copolymers, PEG 120 methyl gluconate dioleate, and PEG 150 distearate. The preservative is any one or more of p-hydroxyacetophenone and octanoyl hydroxamic acid; The pH adjuster is any one or more of tromethamine, triethanolamine, and arginine.

7. The facial cleanser with dual functions of exfoliation and blackhead removal as described in claim 1, characterized in that, In the inner tube material, the amino acid-type surfactant is any one or more of the following: potassium cocoyl glycinate, potassium lauroyl glycinate, TEA salt of cocoyl glycinate, sodium cocoyl glycinate, sodium lauroyl glycinate, sodium cocoyl glutamate, TEA salt of cocoyl glutamate, sodium lauroyl sarcosinate, disodium cocoyl glutamate, sodium cocoyl aminopropionate, and sodium methyl cocoyl taurate. The non-amino acid surfactant is any one or more of sodium lauroyl taurate, potassium cocoyl hydrolyzed collagen, cocamidopropyl hydroxysulfonate, and sodium lauroyl amphoteric acetate. The thickener is any one or more of the following: modified corn starch, hydroxypropyl starch phosphate, PEG-120 methyl glucoside ester, and sodium acrylate / sodium acryloyl dimethyl taurate copolymer. The humectant is any one or both of glycerin and 1,2-hexanediol; The preservative is any one or more of p-hydroxyacetophenone and octanoyl hydroxamic acid.

8. A method for preparing a dual-function facial cleanser for exfoliation and blackhead removal as described in any one of claims 1-7, characterized in that, The preparation method includes preparing an outer tube gel formulation and an inner tube facial cleanser formulation.

9. The preparation method according to claim 8, characterized in that, The preparation method of the outer tube gel formulation includes: S1-1: Mix water, thickener, and humectant, stir, and heat to 80-85℃. Keep warm and stir until completely dissolved, then cool down. S1-2: When the temperature drops to 45~50℃, add the pH adjuster and stir to mix and dissolve evenly; S1-3: When the temperature drops to 40~45℃, add the blackhead removal and exfoliating composition and preservative, stir and mix until evenly dissolved, then discharge. The preparation method of the facial cleanser formulation in the inner tube includes: S2-1: Mix surfactant, water, humectant, fatty acid, thickener, and sodium citrate, stir and heat to 90-95℃, keep warm and stir until completely dissolved, keep warm for 30-40 minutes and then cool down. S2-2: When the temperature drops to 45~50℃, add humectant and preservative, disperse / dissolve evenly, keep warm and stir for 10~15 minutes, and then discharge.

10. The preparation method according to claim 9, characterized in that, The preparation method further includes filling the outer tube gel formulation and the inner tube facial cleanser formulation into a container, with a filling mass ratio of outer tube gel to inner tube facial cleanser of 0.5-5:

1.

11. The use of the product according to any one of claims 1-7 in the preparation of exfoliating and blackhead-removing cosmetics.

12. An exfoliating and blackhead-removing cosmetic, characterized in that, The cosmetic product comprises the product according to any one of claims 1-7.

Images