Bone-strengthening milk protein extract, method for preparing same, and use thereof

By employing pasteurization, casein separation, pepsin and trypsin hydrolysis, and membrane filtration purification, a bone-strengthening bioactive peptide with a specific amino acid sequence of FYPELFR was extracted from skim milk. This method solves the problems of complex preparation processes and low purity in existing technologies, and achieves efficient preparation of high-purity bone-strengthening bioactive peptides, promoting osteoblast proliferation and enhancing the bone-strengthening efficacy of dairy products.

CN120535604BActive Publication Date: 2026-07-24SOUTH CHINA UNIV OF TECH
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Patent Information

Application Number
CN202510737206.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-06-04
Publication Date
2026-07-24
Estimated Expiration
2045-06-04

AI Technical Summary

Technical Problem

In existing technologies, there is a lack of efficient methods for preparing milk-derived bioactive peptides with bone-strengthening functions, especially casein hydrolysate peptides. Furthermore, the preparation process is complex and the purity is low, which fails to meet market demand.

Method used

A bone-strengthening active peptide with a specific amino acid sequence of FYPELFR was extracted from skim milk using pasteurization, casein separation, pepsin and trypsin hydrolysis, and membrane filtration purification. The extract was then screened using LC-ESI-Q-TOF high-resolution liquid chromatography-mass spectrometry and AutoDock Vina software to prepare a high-purity bone-strengthening milk protein extract.

Benefits of technology

The efficient preparation of high-purity bone-strengthening active peptide FYPELFR was achieved. It can bind to integrin αvβ1, promote osteoblast proliferation and differentiation, enhance the bone-strengthening efficacy of dairy products, and expand the application scope of deep processing of dairy products.

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Abstract

The application discloses a bone-strengthening milk protein extract and a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains a bone-strengthening active peptide FYPELFR, and the preparation method specifically comprises the following steps: (1) pasteurization; (2) casein separation; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purification and desalination; and (6) concentration and drying. The bone-strengthening active peptide and the bone-strengthening milk protein extract containing the same can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonding, can promote the proliferation and differentiation of osteoblasts by activating specific signal pathways, can promote the proliferation of osteoblasts, can be used for the development of bone-strengthening functional food or medicine, and has important significance for the deep processing of cow milk.
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