Biochemical sensing system for photoelectrochemical colorimetric detection of circular RNA

The biochemical sensing system catalyzes the hairpin assembly cycle reaction and the G-quadruplex-heme DNA enzyme formation reaction, solves the sensitivity and simplicity problems of circular RNA detection in the existing technology, and realizes highly selective and sensitive photoelectrochemical colorimetric detection, which is suitable for the diagnosis of circular RNA.

CN120665996APending Publication Date: 2025-09-19THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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Patent Information

Application Number
CN202510815851.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2024-06-28
Filing Date
2025-06-18
Publication Date
2025-09-19

AI Technical Summary

Technical Problem

Existing technologies make it difficult to detect low-abundance circular RNAs efficiently, sensitively, and simply, especially because the accuracy of qRT-PCR is affected by primer design and western blot hybridization analysis is time-consuming.

Method used

A biochemical sensing system that catalyzes the hairpin assembly cycle reaction and the G-quadruplex-hemin DNAzyme formation reaction is adopted. Circular RNA is specifically recognized by a specific probe, combined with restriction endonuclease cleavage and magnetic nanoparticle separation to form a G-quadruplex-hemin DNAzyme for signal amplification, which is ultimately detected by photoelectrochemical colorimetry.

Benefits of technology

It achieves highly selective, highly sensitive and simple detection of circular RNA, can accurately distinguish targets in complex samples and provide low-concentration detection, and is suitable for the diagnosis of diseases such as cardiovascular disease and liver cancer.

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Abstract

The invention discloses a biochemical sensing system based on a catalytic hairpin assembly circular reaction and a G quadruplex-heme DNA enzyme formation reaction. The biochemical sensing system is used for photoelectrochemical colorimetric detection of circular RNA (circRNA). After the nucleic acid probe H1 is combined with the nano magnetic beads (MNPs), the circRNA is specifically recognized, and an MNPs / H1 / circRNA compound is formed; the MNPs / H1 / H2 compound reacts with a probe H2 to form an MNPs / H1 / H2 compound; the H1 probe and the H2 probe release a part of sequences by utilizing restriction endonuclease cutting, and the unreleased sequences are combined with hemin to form G quadruplex-heme DNA enzyme with peroxidase-like activity; through cascade catalytic reaction, the color of a solution of a reaction system is changed, the linear relation between the absorbance of the solution and the concentration of circRNA in the solution is fitted, and an effective method for carrying out photoelectrochemical colorimetric detection on circRNA is developed.
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