SNP (Single Nucleotide Polymorphism) marker related to cane sugar component of sugarcane

By developing a SNP marker at the 47469846bp position on sugarcane chromosome 3, the problem of sucrose content control in sugarcane variety selection and breeding was solved, the accurate detection and efficient screening of sugarcane sucrose content were achieved, the accuracy and efficiency of sugarcane breeding were improved, and the selection and breeding of high-sugar sugarcane varieties were promoted.

CN120666095APending Publication Date: 2025-09-19INST OF NANFAN& SEED IND GUANGDONG ACAD OF SCI
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Patent Information

Application Number
CN202511002132.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-07-21
Publication Date
2025-09-19

AI Technical Summary

Technical Problem

The lack of reference sugarcane genome information in existing technologies has increased the difficulty of sugarcane variety selection and breeding, especially the lack of effective molecular markers for controlling sucrose content, making it difficult to quickly and accurately cultivate high-sugar sugarcane varieties.

Method used

A SNP marker related to sugarcane sugar content was developed, located at position 47469846bp on sugarcane chromosome 3. Breeding was carried out by detecting sugarcane samples with AA or AG genotype at this position. Direct sequencing, specific probe hybridization, specific primer extension or PCR were used for detection. Detection methods and kits were provided for screening sugarcane plants with high sugar content and improved sugarcane varieties.

Benefits of technology

It realizes the precise detection and efficient screening of the sucrose content of sugarcane, improves the accuracy and efficiency of sugarcane breeding, can significantly increase the sucrose content of sugarcane, and promotes the selection and breeding of high-sugar sugarcane varieties.

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to cane sugar components of sugarcanes. The inventor finds that genotypes at 47469846bp positions of a sugarcane chromosome 3 are highly related to sugarcane sucrose components, and researches find that the sucrose components of sugarcanes with 91.72% of SNP genotypes AA are all higher than 15.0%, the sucrose components of sugarcanes with 72.73% of SNP genotypes AG are all higher than 15.0%, the sucrose components of sugarcanes with 0.00% of SNP genotypes GG are all higher than 15.0%, and the sucrose components of sugarcanes with 0.00% of SNP genotypes GG are all higher than 15.0%. Therefore, the genotype at the position can be used as the SNP marker of the sucrose component of the sugarcane. By utilizing the discovery, the SNP marker is applied to the detection of the sucrose content of the sugarcane, and the cultivation of a high-sugar sugarcane variety is facilitated.
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