Automatic sampling device and method for evaluating industrial wastewater anaerobic biological toxicity sample

Abstract

The present application relates to the technical fields of anaerobic biological toxicity experiment, and discloses an automatic sample feeding device for industrial wastewater anaerobic biological toxicity evaluation, which comprises a conical flask for temporarily storing anaerobic sludge samples and nutrient solution, and further comprises: a storage box, which is internally provided with a storage space for storing anaerobic sludge samples and nutrient solution; a gas storage tank, which is arranged on the side surface of the storage box and internally filled with high-pressure nitrogen; a nitrogen gas conveying assembly, which is arranged on the gas storage tank and penetrates and extends to the inside of the storage box, and is used for aerating the anaerobic sludge samples in the storage box and realizing sludge homogenization through stirring; and a sample feeding assembly, which is arranged on the storage box and communicates with the storage box, wherein the sample feeding assembly is provided with two sample feeding assemblies for respectively feeding anaerobic sludge samples and nutrient solution into the conical flask. The automatic sample feeding device and method for industrial wastewater anaerobic biological toxicity evaluation aim to simplify the exhaust process, reduce the contact between microorganisms and air, and improve the experimental efficiency and result accuracy.

Description

Technical Field

[0001] This invention relates to the field of anaerobic biological toxicity testing technology, specifically to an automatic sample injection device and method for evaluating the anaerobic biological toxicity of industrial wastewater. Background Technology

[0002] Industrial wastewater is generally characterized by large volume, high toxicity, high concentration, and difficulty in treatment. Anaerobic biological processes, as one of the key technologies in wastewater treatment plants, have been widely applied to industrial wastewater treatment. However, the reaction system is susceptible to the impact of high concentrations of toxic substances in the wastewater, leading to unstable treatment results and even system failure. Therefore, accurately assessing the biotoxicity of wastewater before it enters the wastewater treatment plant is crucial for the efficient operation of the entire wastewater treatment process.

[0003] Current assessments of anaerobic biotoxicity in industrial wastewater primarily rely on anaerobic methanogenesis experiments. The principle involves mixing the wastewater to be assessed with anaerobic microorganisms and quantifying the anaerobic biotoxicity by measuring the amount of methane produced. Existing evaluation systems are manually assembled using equipment such as conical flasks, water baths, simple plastic tubing, and seated fermentation tubes. This operational mode has two significant drawbacks: first, it relies entirely on manual maintenance, resulting in low operational efficiency and susceptibility to human error; second, the system has poor sealing, allowing easy infiltration of outside air. This leads to residual oxygen in the delivery pipes and conical flasks significantly inhibiting the activity of anaerobic microorganisms, severely interfering with the accuracy of the experimental results. Furthermore, the precise addition of anaerobic sludge, wastewater, and nutrient solution plays a decisive role in the assessment results. However, current technologies still rely on manual weighing, which is not only cumbersome but also significantly increases the risk of anaerobic sludge deactivation due to frequent exposure to the air environment. Additionally, it is difficult to guarantee high-precision volumetric measurement, resulting in poor reliability and repeatability of experimental data. Based on the above problems, there is an urgent need to invent an anaerobic biotoxicity assessment system that can accurately sample and reduce the contact between air and anaerobic sludge, so as to achieve efficient assessment of the biotoxicity of industrial wastewater. Summary of the Invention

[0004] The purpose of this invention is to simplify the exhaust process, reduce the contact between microorganisms and air, and improve experimental efficiency and accuracy. Therefore, an automatic sample injection device and method for evaluating the anaerobic biological toxicity of industrial wastewater are proposed.

[0005] The technical solution of the present invention to solve the above-mentioned technical problems is as follows:

[0006] An automated sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater includes a conical flask for temporarily storing anaerobic sludge samples and nutrient solution, and further includes:

[0007] The storage tank contains storage space for storing anaerobic sludge samples and nutrient solution.

[0008] A gas storage tank is located on the side surface of the storage box and is filled with high-pressure nitrogen.

[0009] A nitrogen delivery assembly is installed on the gas storage tank and extends through and into the inside of the storage box. It is used to aerate the anaerobic sludge sample in the storage box and to homogenize the sludge by stirring.

[0010] The sample supply assembly is located on the storage tank and is connected to the storage tank. There are two sample supply assemblies to supply anaerobic sludge samples and nutrient solution into the conical flasks respectively.

[0011] A gas-driven assembly, connected to the storage tank and interconnected with the supply end of the sample supply assembly, is used to position and seal the opening of the conical flask and drive the supply end of the sample supply assembly to move toward the opening of the conical flask.

[0012] The pipeline connection component is connected to the gas drive component and is interconnected with the sample supply component. It is used to replace the air in the sample supply component and the conical flask with nitrogen, thereby eliminating the interference of air on the anaerobic sludge sample and the experimental process.

[0013] Based on the above technical solution, the present invention can be further improved as follows.

[0014] Furthermore, a partition perpendicular to the horizontal plane is fixedly installed inside the storage box, which divides the internal area of ​​the storage box into an anaerobic sludge sample storage area and a nutrient solution storage area. Two feeding pipes that penetrate through and extend into the inside of the storage box are also fixedly installed on the outside of the storage box, with the two feeding pipes corresponding to the anaerobic sludge sample storage area and the nutrient solution storage area, respectively.

[0015] Furthermore, the nitrogen delivery assembly includes:

[0016] A chamber is fixedly installed on the inner wall of the storage box. A first air pipe is also connected to the chamber, with one end penetrating through and extending to the outside of the storage box. The other end of the first air pipe is connected to the air outlet of the air tank. An output port is also provided on the surface of the chamber, which is opposite to the first air pipe.

[0017] The first solenoid valve is installed on the first air pipe and is used to control the opening and closing of the first air pipe.

[0018] A pneumatic drive component is located inside the chamber. The pneumatic drive component is also equipped with a stirring rod. High-pressure nitrogen gas in the gas storage tank is input into the chamber through the first gas pipe to drive the pneumatic drive component and the stirring rod to operate, so as to stir the anaerobic sludge to ensure the homogeneity of the sludge, and output it from the outlet to aerate the anaerobic sludge sample in the storage tank.

[0019] Furthermore, the pneumatic actuation component includes:

[0020] The first gear is rotatably connected to the inside of the chamber;

[0021] The shaft is rotatably connected to the inside of the chamber and one end extends through and to the outside of the chamber. The end of the stirring rod is fixedly connected to the end of the shaft located outside the chamber.

[0022] The second gear is fixedly installed on the outside of the shaft and meshes with the first gear.

[0023] Furthermore, the sample supply assembly includes:

[0024] The pump body is fixedly installed on the outside of the storage tank. The suction end of the pump body is connected to a suction pipe that extends through and into the inside of the storage tank. There are two pump bodies and two suction pipes. The two suction pipes are respectively set in the anaerobic sludge sample storage area and the nutrient solution storage area.

[0025] A connecting seat is provided on the output end of the pump body and is connected to the output end of the pump body;

[0026] A filling tube, one end of which is connected to a connecting seat. The number of filling tubes is not less than eight and is divided into two groups, which are respectively set on two connecting seats. The outer sides of two adjacent filling tubes are fixedly connected to rubber plugs. The number of rubber plugs is four, and each rubber plug is provided with two filling tubes from the two connecting seats respectively.

[0027] The flow valves are fixedly installed on the outside of the filling pipe, and their number and distribution are matched with the filling pipe.

[0028] Furthermore, the gas-driven assembly includes:

[0029] The main exhaust pipe is fixedly installed on the outside of the storage box and is connected to the anaerobic sludge sample storage area.

[0030] A piston cylinder is fixedly installed on the outside of the storage box. A piston rod is provided on the inside of the piston cylinder, with one end penetrating and extending to the outside of the piston cylinder. The piston rod can extend and retract along the inside of the piston cylinder. There are two piston cylinders, and the two piston cylinders are connected to each other through a connecting pipe.

[0031] An elastic element, one end of which is fixedly installed inside the piston cylinder, and the other end is fixedly connected to the end of the piston rod.

[0032] The second air pipe has one end connected to the main air outlet pipe and the other end connected to the piston cylinder. A second solenoid valve is also provided on the outside of the second air pipe.

[0033] A venting solenoid valve is mounted on the piston cylinder and is connected to the piston cylinder.

[0034] The mounting plate is fixedly installed on the end of the piston rod located outside the piston cylinder, and the rubber plug is fixedly connected to the mounting plate.

[0035] Furthermore, the pipeline connection component includes:

[0036] The third vent pipe has one end connected to the main vent pipe and the other end extending through and into one of the connecting seats. A third solenoid valve is provided on the third vent pipe.

[0037] An interconnecting pipe connects two connecting seats, and a fourth solenoid valve is provided on the outside of the interconnecting pipe.

[0038] Furthermore, an output tube is also provided through the rubber stopper, and the other end of the output tube is connected to a gas quantity measuring component.

[0039] Furthermore, the gas storage tank is also equipped with a gas filling port.

[0040] An automated sample injection method for evaluating the anaerobic toxicity of industrial wastewater includes the following steps:

[0041] S10. Anaerobic sludge sample and nutrient solution are added to the anaerobic sludge sample storage area and nutrient solution storage area in the storage tank through the feeding pipe. The first solenoid valve is opened, and the high-pressure nitrogen in the gas storage tank is input into the chamber of the nitrogen delivery component through the first gas pipe. The first gear and the second gear of the pneumatic drive component mesh with each other, driving the shaft to rotate axially, so that the stirring rod stirs the anaerobic sludge to ensure the homogeneity of the sludge. At the same time, nitrogen is output from the outlet of the chamber to aerate the anaerobic sludge sample in the storage tank.

[0042] S20. Open the second solenoid valve of the gas-driven component. Nitrogen gas in the anaerobic sludge sample storage area is output to the inside of the piston cylinder through the main outlet pipe and the second gas pipe. Under the action of gas pressure, the piston rod is pushed outward. Place the conical bottle directly under the rubber stopper. After the piston rod is fully extended, close the second solenoid valve and the venting solenoid valve.

[0043] S30. Open the venting solenoid valve, and the gas in the piston cylinder is discharged. Under the action of the elastic element, the rubber stopper is pressed down at the opening of the conical bottle.

[0044] S40. Open the third and fourth solenoid valves of the pipeline connection assembly. Nitrogen gas in the anaerobic sludge sample storage area is output to the inside of one of the connecting seats through the main outlet pipe and the third outlet pipe. Nitrogen gas is also introduced into the other connecting seat through the interconnection pipe. Nitrogen gas is injected into the conical flask from the connecting seat and the filling pipe, replacing the air in the connecting seat, the filling pipe and the conical flask with nitrogen gas. During this process, the gas volume measurement component connected to the output pipe on the rubber stopper measures whether the air has been completely replaced.

[0045] S50. Turn on the pump body and flow valve of the sample supply component. The two pump bodies draw in anaerobic sludge samples and nutrient solutions from the anaerobic sludge sample storage area and nutrient solution storage area of ​​the storage tank through the suction pipe, respectively, and inject them into the conical flask through the connecting seat and the filling pipe to complete the automatic sample injection for the anaerobic toxicity evaluation of industrial wastewater.

[0046] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0047] In the automatic sample injection device for anaerobic biological toxicity evaluation of industrial wastewater of the present invention, a storage tank can store anaerobic sludge samples and nutrient solution. An air storage tank works in conjunction with a nitrogen delivery component to aerate and stir the anaerobic sludge samples in the storage tank using high-pressure nitrogen, achieving sludge homogenization. This ensures the uniformity of the sludge samples and improves the reliability of experimental results. Furthermore, the aeration process reduces residual air in the sludge to some extent, mitigating the impact of air on microbial activity. Two sample supply components supply anaerobic sludge samples and nutrient solution to conical flasks, respectively, ensuring consistency in sample and nutrient solution quantities. A gas-driven component carries the conical flask and drives the supply end of the supply component to move. Combined with a pipeline connection component, it can automatically replace the air in the sample supply components and conical flasks with nitrogen. This system not only simplifies the process and improves experimental efficiency but also ensures the consistency and thoroughness of the venting operation, effectively eliminating air interference with the anaerobic sludge samples and experimental process, ensuring the activity of anaerobic microorganisms, and thus improving the accuracy and reliability of the anaerobic toxicity evaluation experimental results of industrial wastewater. Attached Figure Description

[0048] Figure 1 This is a schematic diagram of the overall connection structure of the present invention;

[0049] Figure 2 This is a schematic diagram of the connection structure from another perspective of the present invention;

[0050] Figure 3 This is a schematic diagram of the internal cross-sectional connection structure of the storage box of the present invention;

[0051] Figure 4 This is a schematic diagram of the connection structure between the storage box and the feeding pipe of the present invention;

[0052] Figure 5 This is a schematic diagram of the connection structure of some gas-driven components of the present invention;

[0053] Figure 6 This is a schematic diagram of the internal structure of the cavity in this invention.

[0054] In the diagram: 1. Conical flask; 2. Storage tank; 3. Gas storage tank; 4. Nitrogen delivery assembly; 41. Chamber; 42. First gas pipe; 43. Outlet; 44. First solenoid valve; 45. Pneumatic drive component; 451. First gear; 452. Shaft; 453. Second gear; 46. Stirring rod; 5. Sample supply assembly; 51. Pump body; 52. Suction pipe; 53. Connecting seat; 54. Filling pipe; 55. Rubber stopper; 56. Flow valve 6. Gas drive assembly; 61. Main gas outlet pipe; 62. Piston cylinder; 63. Piston rod; 64. Elastic element; 65. Second gas pipe; 66. Second solenoid valve; 67. Venting solenoid valve; 68. Mounting plate; 7. Pipeline connection assembly; 71. Third gas outlet pipe; 72. Third solenoid valve; 73. Interconnection pipe; 74. Fourth solenoid valve; 8. Baffle plate; 9. Feed pipe; 10. Output pipe; 11. Gas quantity measurement assembly; 12. Gas inlet. Detailed Implementation

[0055] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0056] Combination Figures 1-6 As shown, an automatic sample injection device for evaluating the anaerobic biological toxicity of industrial wastewater according to the present invention includes a conical flask 1 for temporarily storing anaerobic sludge samples and nutrient solution, and further includes:

[0057] Storage tank 2, which has a storage space for storing anaerobic sludge samples and nutrient solution;

[0058] Gas storage tank 3 is located on the side surface of storage tank 2 and is filled with high-pressure nitrogen.

[0059] Nitrogen delivery component 4 is installed on gas storage tank 3 and extends through and into the inner side of storage box 2. It is used to aerate the anaerobic sludge sample in storage box 2 and to achieve sludge homogenization by stirring.

[0060] The sample supply component 5 is installed on the storage tank 2 and is connected to the storage tank 2. There are two sample supply components 5 to supply anaerobic sludge samples and nutrient solution into the conical flask 1 respectively.

[0061] The gas-driven assembly 6 is connected to the storage tank 2 and is interconnected with the supply end of the sample supply assembly 5. It is used to position and seal the opening of the conical flask 1 and drive the supply end of the sample supply assembly 5 to move toward the opening of the conical flask 1.

[0062] The pipeline connection component 7 is connected to the gas drive component 6 and is interconnected with the sample supply component 5. It is used to replace the air in the sample supply component 5 and the conical flask 1 with nitrogen, thereby eliminating the interference of air on the anaerobic sludge sample and the experimental process.

[0063] When this automatic sampling device for anaerobic biological toxicity evaluation of industrial wastewater is in operation, the anaerobic sludge sample and nutrient solution are first stored in the storage space of storage tank 2. High-pressure nitrogen stored in gas tank 3 is introduced into the inside of storage tank 2 through nitrogen delivery component 4. This aerates the anaerobic sludge sample and drives the internal stirring structure to homogenize the sludge and ensure the uniformity of the sludge sample. Before sampling, gas drive component 6 carries conical flask 1, and pipeline connection component 7 is activated. Using nitrogen provided in storage tank 2 or gas tank 3, the air inside sampling supply component 5 and conical flask 1 is replaced with nitrogen, eliminating the interference of air on the anaerobic sludge sample and subsequent experimental process, and creating a stable anaerobic environment. After the replacement is completed, the two sampling supply components 5 respectively draw anaerobic sludge sample and nutrient solution from storage tank 2, preparing to supply them into conical flask 1, completing the automatic sampling operation and providing sample preparation for subsequent accurate anaerobic toxicity evaluation experiments of industrial wastewater.

[0064] In a preferred embodiment, the present invention may be further configured as follows: Figure 3 , Figure 4 As shown, a partition 8 perpendicular to the horizontal plane is fixedly installed inside the storage tank 2. The partition 8 divides the internal area of ​​the storage tank 2 into an anaerobic sludge sample storage area and a nutrient solution storage area. Two feed pipes 9 are also fixedly installed on the outside of the storage tank 2, penetrating and extending into the inside of the storage tank 2. These two feed pipes 9 correspond to the anaerobic sludge sample storage area and the nutrient solution storage area, respectively. The partition 8, fixedly installed perpendicular to the horizontal plane, divides the interior of the storage tank 2 into two independent and equal-sized areas for storing anaerobic sludge samples and nutrient solutions, respectively. During the experimental preparation stage, the operator can add the corresponding substances to the anaerobic sludge sample storage area and the nutrient solution storage area through the two feed pipes 9 on the outside of the storage tank 2. Due to the separating effect of the partition 8, the anaerobic sludge sample and nutrient solution are prevented from mixing within the storage tank 2, maintaining the independence of the two substances.

[0065] In a preferred embodiment, the present invention may be further configured as follows: Figure 3 , Figure 6 As shown; the nitrogen delivery assembly 4 includes:

[0066] The chamber 41 is fixedly installed on the inner wall of the storage box 2. A first air pipe 42 is also connected to the chamber 41, with one end penetrating through and extending to the outside of the storage box 2. The other end of the first air pipe 42 is connected to the air outlet of the air tank 3. An output port 43 is also provided on the surface of the chamber 41, which is opposite to the first air pipe 42.

[0067] The first solenoid valve 44 is disposed on the first air pipe 42 and is used to control the opening and closing of the first air pipe 42.

[0068] A pneumatic drive component 45 is located inside the chamber 41. A stirring rod 46 is also mounted on the pneumatic drive component 45. High-pressure nitrogen from the gas storage tank 3 is input into the chamber 41 through the first gas pipe 42 to drive the pneumatic drive component 45 and the stirring rod 46 to rotate, thereby agitating the anaerobic sludge to ensure its homogeneity. The nitrogen is then output from the outlet 43 to aerate the anaerobic sludge sample in the storage tank 2. When the anaerobic sludge sample in the storage tank 2 needs to be processed, the first solenoid valve 44 is opened. The high-pressure nitrogen from the gas storage tank 3 is then transported through the first gas pipe 42 to the chamber 41, which is fixedly installed on the inner wall of the storage tank 2. The high-pressure nitrogen entering the chamber 41 drives the pneumatic drive component 45 to rotate. Since the stirring rod 46 is located on the pneumatic drive component 45... The pneumatic drive on component 45 causes the stirring rod 46 to rotate synchronously, thereby stirring the anaerobic sludge in storage tank 2, achieving sludge homogenization, ensuring uniform mixing of the anaerobic sludge sample, improving sample consistency and the reliability of experimental results. At the same time, after high-pressure nitrogen completes its stirring function in chamber 41, it is discharged from the output port 43, which is set opposite to the first air pipe 42, to aerate the anaerobic sludge sample in storage tank 2. The aeration process can provide a certain gas environment for the anaerobic sludge, and also help to further disperse the sludge, enhance the stirring and homogenization effect, and reduce the residual air that may exist in the sludge, reducing the impact of air on the activity of anaerobic microorganisms.

[0069] In a preferred embodiment, the present invention may be further configured as follows: Figure 3 , Figure 6 As shown; the pneumatic drive component 45 includes:

[0070] The first gear 451 is rotatably connected to the inside of the chamber 41;

[0071] The shaft 452 is rotatably connected to the inside of the chamber 41 and one end extends through and to the outside of the chamber 41. The end of the stirring rod 46 is fixedly connected to the end of the shaft 452 located outside the chamber 41.

[0072] The second gear 453 is fixedly installed on the outside of the shaft 452 and meshes with the first gear 451. When the high-pressure nitrogen gas in the gas storage tank 3 enters the chamber 41 through the first gas pipe 42, the airflow with a certain pressure and velocity directly acts on the first gear 451, which is rotatably connected to the inside of the chamber 41, pushing the first gear 451 and the second gear 453 to start rotating. The rotation of the second gear 453 drives the shaft 452 to rotate axially, thereby driving the stirring rod 46 to move in a circular motion around the axis of the shaft 452. It should also be noted that the structure and principle of the chamber 41, the first gear 451, and the second gear 453 are the same as those of the gear-type pneumatic motor in the prior art, and will not be described again here. The direction of airflow is as follows: Figure 6 As shown, the first gear 451 and the second gear 453 can be driven to mesh and rotate in the direction of airflow.

[0073] In a preferred embodiment, the present invention may be further configured as follows: Figure 2 , Figure 6 As shown; the sample supply assembly 5 includes:

[0074] The pump body 51 is fixedly installed on the outside of the storage tank 2. The suction end of the pump body 51 is connected to a suction pipe 52 that extends through and into the inside of the storage tank 2. There are two pump bodies 51 and two suction pipes 52. The two suction pipes 52 are respectively set in the anaerobic sludge sample storage area and the nutrient solution storage area. The suction pipes 52 are close to the inner wall of the storage tank 2, so as not to affect the normal operation of the stirring rod 46.

[0075] The connecting seat 53 is disposed on the output end of the pump body 51 and is connected to the output end of the pump body 51.

[0076] A filling tube 54, one end of which is connected to a connecting seat 53. There are no fewer than eight filling tubes 54, which are divided into two groups and respectively set on two connecting seats 53. The outer sides of two adjacent filling tubes 54 are fixedly connected to rubber stoppers 55. There are four rubber stoppers 55. Each rubber stopper 55 is provided with two filling tubes 54 from the two connecting seats 53 respectively. The rubber stoppers 55 are adapted to the mouth diameter of the conical flask 1.

[0077] Flow valves 56 are fixedly installed on the outside of the filling pipes 54, and their number and distribution are matched one-to-one with the filling pipes 54. Two pump bodies 51 are fixed on the outside of the storage tank 2. Their suction ends are inserted into the anaerobic sludge sample storage area and nutrient solution storage area of ​​the storage tank 2 through suction pipes 52, respectively. When the pump body 51 is started, it uses negative pressure suction to draw anaerobic sludge samples and nutrient solutions from the corresponding storage areas through the suction pipes 52 and delivers them to the output end of the pump body 51. The connecting seat 53 connected to the output end of the pump body 51 plays the role of collection and diversion, introducing the samples and nutrient solutions output by the pump body 51 into multiple filling pipes 54. No less than eight filling pipes 54 are divided into two groups and connected to two connecting seats 53 respectively. These filling pipes 54 further deliver the samples and nutrient solutions into the conical flask 1. The outer side of the injection tube 54 is fixed to the rubber stopper 55. The four rubber stoppers 55 can be adapted to the mouth of the conical flask 1. The two injection tubes 54 on each rubber stopper 55 correspond to the delivery channels of the anaerobic sludge sample and the nutrient solution, respectively, to realize the synchronous injection of the conical flask 1. The flow valve 56 fixedly installed on the outside of the injection tube 54 can accurately adjust the flow rate and delivery volume of the sample and nutrient solution in the injection tube 54 according to the experimental requirements. By controlling the opening of the flow valve 56, on the one hand, it can ensure that the amount of sludge sample and nutrient solution in the experimental group and each control group is kept at a small difference, avoiding excessive contact between microorganisms and air due to manual weighing. On the other hand, it can also ensure that an appropriate amount of sample and nutrient solution is injected into the conical flask 1 within the specified time, providing accurate and compliant samples for the anaerobic toxicity evaluation experiment of industrial wastewater. The flow rates of the two pumps 51 are the same.

[0078] In a preferred embodiment, the present invention may be further configured as follows: Figure 1 , Figure 5 As shown; the gas-driven assembly 6 includes:

[0079] The main vent pipe 61 is fixedly installed on the outside of the storage box 2 and is connected to the anaerobic sludge sample storage area.

[0080] Piston cylinder 62 is fixedly installed on the outside of storage box 2. A piston rod 63 is provided on the inside of piston cylinder 62, with one end penetrating and extending to the outside of piston cylinder 62. Piston rod 63 can extend and retract along piston cylinder 62. There are two piston cylinders 62, and the two piston cylinders 62 are connected to each other through a connecting pipe.

[0081] The elastic element 64 has one end fixedly installed inside the piston cylinder 62, and the other end fixedly connected to the end of the piston rod 63.

[0082] The second air pipe 65 has one end connected to the main air outlet pipe 61 and the other end connected to the piston cylinder 62. A second solenoid valve 66 is also provided on the outside of the second air pipe 65.

[0083] A venting solenoid valve 67 is mounted on the piston cylinder 62 and is connected to the piston cylinder 62.

[0084] Mounting plate 68 is fixedly mounted on the end of piston rod 63 located outside piston cylinder 62. Rubber plug 55 is fixedly connected to mounting plate 68. During the sample preparation stage for anaerobic toxicity evaluation of industrial wastewater, gas drive assembly 6 starts to operate, opening the second solenoid valve 66. Nitrogen gas in the anaerobic sludge sample storage area of ​​storage tank 2 enters the inside of piston cylinder 62 through main outlet pipe 61 and second gas pipe 65. Since the two piston cylinders 62 are interconnected through connecting pipes, nitrogen gas enters the two piston cylinders 62 evenly. The gas pressure pushes piston rod 63 to overcome the resistance of elastic element 64 and extend outward along piston cylinder 62. Mounting plate 68 fixed to the outer end of piston rod 63 drives rubber plug 55 connected to it. 5. When the piston rod 63 is fully extended to the outside of the piston cylinder 62, close the second solenoid valve 66 and the venting solenoid valve 67 to prevent nitrogen from continuing to enter the piston cylinder 62 and to prevent internal gas leakage. Maintain the extended state of the piston rod 63 to place the conical bottle 1. After the conical bottle 1 is placed, open the venting solenoid valve 67. The nitrogen in the piston cylinder 62 is discharged, the internal gas pressure drops, the elastic element 64 loses the pressure restraint, and the elastic force generated by the return to the original state pulls the piston rod 63 back into the piston cylinder 62, causing the mounting plate 68 and the rubber stopper 55 to move downward, so that the rubber stopper 55 is tightly fastened to the opening of the conical bottle 1.

[0085] In a preferred embodiment, the present invention may be further configured as follows: Figure 2 , Figure 4 As shown; the pipeline connection component 7 includes:

[0086] The third vent pipe 71 has one end connected to the main vent pipe 61 and the other end extending through and into one of the connecting seats 53. A third solenoid valve 72 is provided on the third vent pipe 71.

[0087] An interconnecting pipe 73 connects two connecting seats 53. A fourth solenoid valve 74 is installed on the outside of the interconnecting pipe 73. During the sample preparation stage of the industrial wastewater anaerobic toxicity evaluation experiment, the pipeline connection component 7 is activated to eliminate air interference in the sample injection system. The third solenoid valve 72 and the fourth solenoid valve 74 are opened. Nitrogen gas in the anaerobic sludge sample storage area of ​​the storage tank 2 enters one of the connecting seats 53 through the main vent pipe 61 and the third vent pipe 71. Since the two connecting seats 53 are connected by the interconnecting pipe 73, the nitrogen gas quickly diffuses to the other connecting seat 53, filling both connecting seats 53 with nitrogen gas. Subsequently, the nitrogen gas is injected into the placed sample via the injection pipe 54 connected to the connecting seat 53. Inside the conical flask 1, which is in place and sealed with a rubber stopper 55, as nitrogen is continuously injected, the air originally present in the connecting seat 53, the filling tube 54, and the conical flask 1 is gradually replaced and discharged, forming a high-purity nitrogen environment. This effectively avoids the inhibitory effect of residual air on the microbial activity in the anaerobic sludge sample. During the sample injection stage, the fourth solenoid valve 74 is closed, allowing the two connecting seats 53 to operate independently. This ensures that the anaerobic sludge sample and nutrient solution do not interfere with each other when injected into the conical flask 1 through their respective filling tubes 54, preventing cross-contamination. The third solenoid valve 72 controls the flow of nitrogen and is closed after the air replacement is completed, preventing nitrogen from affecting the stability of the fluid delivery pressure and flow rate during subsequent sample injection.

[0088] In a preferred embodiment, the present invention may be further configured as follows: Figure 1 , Figure 2 As shown; an output pipe 10 is also installed through the rubber stopper 55, and the other end of the output pipe 10 is connected to a gas volume measuring component 11. In the air replacement stage of the anaerobic toxicity evaluation experiment of industrial wastewater, when the pipeline connecting component 7 injects nitrogen into the connecting seat 53, the filling pipe 54 and the conical flask 1 to replace the air, the output pipe 10 installed through the rubber stopper 55 is connected to the gas volume measuring component 11, playing a key monitoring role. After the nitrogen enters the system, it pushes the original air out of the output pipe 10 and flows to the gas volume measuring component 11. The gas volume measuring component 11 monitors the flow rate, volume and other parameters of the discharged gas in real time. By analyzing these data, it determines the air volume in the connecting seat 53, the filling pipe 54 and the conical flask 1. Whether the gas has been completely replaced with nitrogen is determined by the measurement data. If the gas composition is stable and meets the index of pure nitrogen, it indicates that the air replacement is complete. If the data is abnormal, it indicates that there is still residual air. At this time, the nitrogen injection volume and replacement time can be adjusted until the gas volume measurement component 11 reports that the air has been completely replaced. In this way, the cooperation between the output pipe 10 and the gas volume measurement component 11 ensures the anaerobic environment required for the experiment, effectively avoiding the influence of residual air on the activity of anaerobic microorganisms and reducing interference with the accuracy of the anaerobic toxicity evaluation results of industrial wastewater. The gas volume measurement component 11 is one of the gas flow meters and gas analyzers. The gas volume measurement component 11 is connected to the end of the output pipe 10 through a quick-release connector.

[0089] In a preferred embodiment, the present invention may be further configured as follows: Figure 1 , Figure 2 As shown; the gas storage tank 3 is also equipped with a gas filling port 12. During the anaerobic toxicity evaluation experiment of industrial wastewater, the gas storage tank 3 serves as a storage container for high-pressure nitrogen. The nitrogen inside the tank is continuously consumed as the tank 2 is aerated and the air is replaced. When the nitrogen storage in the gas storage tank 3 is insufficient to meet the nitrogen demand of subsequent experiments, the gas filling port 12 comes into play. The operator can connect to the gas filling port 12 through a dedicated nitrogen delivery device and use the device to inject high-pressure nitrogen into the gas storage tank 3 to replenish the nitrogen storage in the tank. The timely replenishment of nitrogen through the gas filling port 12 can ensure that the nitrogen delivery component 4 continuously aerates and stirs the anaerobic sludge sample in the storage tank 2 to achieve sludge homogenization, and ensures that the pipeline connection component 7 can smoothly replace the air in the sample supply component 5 and the conical flask 1 with nitrogen, thereby maintaining the stable operation of the entire automatic sample feeding device.

[0090] Storage tank 2 is equipped with partitions 8 to separate areas and a feeding pipe 9, allowing for independent storage of anaerobic sludge samples and nutrient solution, preventing them from mixing and deteriorating, and reducing contact with outside air. The gas storage tank 3 and nitrogen delivery assembly 4 utilize high-pressure nitrogen to aerate the sludge and drive the stirring rod 46 to homogenize it, ensuring sample uniformity and reducing the impact of air on the activity of anaerobic microorganisms. The sample feeding assembly 5 uses a dual-pump body 51 with a flow valve 56 to precisely control the sample volume, avoiding frequent contact between microorganisms and air caused by manual weighing, ensuring the uniformity of the experimental and control group samples. Consistent quality reduces experimental errors; the gas-driven assembly 6 uses nitrogen pressure to drive the piston rod 63, achieving automatic fixation and sealing of the rubber stopper 55 to the conical flask 1, simplifying the operation process; the pipeline connection assembly 7 uses nitrogen to replace the air in the sample injection system, and together with the output tube 10 on the rubber stopper 55 and the gas quantity measurement assembly 11, it can accurately monitor the degree of air replacement and create a stable anaerobic environment; the gas filling port 12 of the gas storage tank 3 ensures a continuous supply of nitrogen and maintains stable operation of the device. This device has a compact structure, and the modules can be integrated, which is convenient for batch preparation and on-site deployment.

[0091] An automated sample injection method for evaluating the anaerobic toxicity of industrial wastewater includes the following steps:

[0092] S10. Anaerobic sludge sample and nutrient solution are added to the anaerobic sludge sample storage area and nutrient solution storage area in the storage tank 2 through the feeding pipe 9 respectively. The first solenoid valve 44 is opened, and the high-pressure nitrogen in the gas storage tank 3 is input into the chamber 41 of the nitrogen delivery component 4 through the first gas pipe 42. The first gear 451 and the second gear 453 of the pneumatic drive component 45 mesh with each other, driving the shaft 452 to rotate axially, so that the stirring rod 46 stirs the anaerobic sludge to ensure the homogeneity of the sludge. At the same time, nitrogen is output from the outlet 43 of the chamber 41 to aerate the anaerobic sludge sample in the storage tank 2.

[0093] S20. Open the second solenoid valve 66 of the gas drive assembly 6. Nitrogen gas in the anaerobic sludge sample storage area is output to the inside of the piston cylinder 62 through the main outlet pipe 61 and the second gas pipe 65. Under the action of gas pressure, the piston rod 63 is pushed outward. The conical bottle 1 is placed directly below the rubber stopper 55. After the piston rod 63 is fully extended, close the second solenoid valve 66 and the venting solenoid valve 67.

[0094] S30. Open the venting solenoid valve 67, and the gas in the piston cylinder 62 is discharged. Under the action of the elastic element 64, the rubber stopper 55 is pressed down onto the opening of the conical bottle 1.

[0095] S40. Open the third solenoid valve 72 and the fourth solenoid valve 74 of the pipeline connection component 7. Nitrogen in the anaerobic sludge sample storage area is output to the inside of one of the connecting seats 53 through the main outlet pipe 61 and the third outlet pipe 71. Nitrogen is also filled into the other connecting seat 53 through the interconnection pipe 73. Nitrogen is injected into the conical flask 1 from the connecting seat 53 and the filling pipe 54, replacing the air in the connecting seat 53, the filling pipe 54 and the conical flask 1 with nitrogen. During this process, the gas quantity measurement component 11 connected to the output pipe 10 on the rubber stopper 55 measures whether the air has been completely replaced.

[0096] S50, turn on the pump body 51 and flow valve 56 of the sample supply component 5. The two pump bodies 51 respectively draw anaerobic sludge samples and nutrient solutions from the anaerobic sludge sample storage area and nutrient solution storage area of ​​the storage tank 2 through the suction pipe 52, and inject them into the conical flask 1 through the connecting seat 53 and the filling pipe 54 to complete the automatic sample injection for the anaerobic toxicity evaluation of industrial wastewater.

[0097] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0098] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automatic sample injection device for evaluating the anaerobic biological toxicity of industrial wastewater, comprising a conical flask (1) for temporarily storing anaerobic sludge samples and nutrient solution, characterized in that, Also includes: Storage box (2), which has a storage space for storing anaerobic sludge samples and nutrient solution; A gas storage tank (3) is installed on the side surface of the storage box (2), and high-pressure nitrogen is injected inside it; The nitrogen delivery component (4) is installed on the gas storage tank (3) and extends through and into the inner side of the storage box (2). It is used to aerate the anaerobic sludge sample in the storage box (2) and to achieve sludge homogenization by stirring. The sample supply component (5) is set on the storage box (2) and is connected to the storage box (2). There are two sample supply components (5) to supply anaerobic sludge samples and nutrient solution into the conical flask (1) respectively. The gas-driven assembly (6) is connected to the storage tank (2) and is connected to the supply end of the sample supply assembly (5). It is used to position and seal the opening of the conical flask (1) and drive the supply end of the sample supply assembly (5) to move toward the opening of the conical flask (1). The pipeline connection component (7) is connected to the gas drive component (6) and is interconnected with the sample supply component (5). It is used to replace the air in the sample supply component (5) and the conical flask (1) with nitrogen to eliminate the interference of air on the anaerobic sludge sample and the experimental process. The storage box (2) is fixedly installed with a partition (8) perpendicular to the horizontal plane. The partition (8) divides the internal area of ​​the storage box (2) into an anaerobic sludge sample storage area and a nutrient solution storage area. The storage box (2) is also fixedly installed with two feeding pipes (9) that penetrate and extend to the inside of the storage box (2). The two feeding pipes (9) correspond to the anaerobic sludge sample storage area and the nutrient solution storage area, respectively. The nitrogen delivery assembly (4) includes: The chamber (41) is fixedly installed on the inner wall of the storage box (2). A first air pipe (42) is also connected to the chamber (41) with one end penetrating through and extending to the outside of the storage box (2). The other end of the first air pipe (42) is connected to the air outlet of the air tank (3). An output port (43) is also provided on the surface of the chamber (41) opposite to the first air pipe (42). The first solenoid valve (44) is installed on the first air pipe (42) and is used to control the opening and closing of the first air pipe (42); A pneumatic drive component (45) is provided inside the chamber (41). A stirring rod (46) is also provided on the pneumatic drive component (45). High-pressure nitrogen in the gas storage tank (3) is input into the chamber (41) through the first gas pipe (42) to drive the pneumatic drive component (45) and the stirring rod (46) to operate, so as to stir the anaerobic sludge to ensure the homogeneity of the sludge, and output from the outlet (43) to aerate the anaerobic sludge sample in the storage tank (2). The sample supply assembly (5) includes: The pump body (51) is fixedly installed on the outside of the storage tank (2). The suction end of the pump body (51) is connected to a suction pipe (52) that extends through and into the storage tank (2). There are two pump bodies (51) and two suction pipes (52). The two suction pipes (52) are respectively set in the anaerobic sludge sample storage area and the nutrient solution storage area. A connecting seat (53) is provided on the output end of the pump body (51) and is connected to the output end of the pump body (51); A filling tube (54) is provided, one end of which is connected to a connecting seat (53). The number of filling tubes (54) is not less than eight and is divided into two groups, which are respectively set on two connecting seats (53). The outer sides of two adjacent filling tubes (54) are fixedly connected to rubber plugs (55). The number of rubber plugs (55) is four, and each rubber plug (55) is provided with two filling tubes (54) from the two connecting seats (53) respectively. Flow valves (56) are fixedly installed on the outside of the filling pipe (54), and their number and distribution positions are matched one by one with the filling pipe (54); The gas-driven assembly (6) includes: The main exhaust pipe (61) is fixedly installed on the outside of the storage box (2) and is connected to the anaerobic sludge sample storage area. A piston cylinder (62) is fixedly installed on the outside of the storage box (2). A piston rod (63) with one end penetrating through and extending to the outside of the piston cylinder (62) is provided on the inside of the piston cylinder (62). The piston rod (63) can extend and retract along the inside of the piston cylinder (62). There are two piston cylinders (62), and the two piston cylinders (62) are connected to each other through a connecting pipe. The elastic element (64) has one end fixedly installed on the inside of the piston cylinder (62), and the other end is fixedly connected to the end of the piston rod (63); The second air pipe (65) has one end connected to the main air outlet pipe (61) and the other end connected to the piston cylinder (62). A second solenoid valve (66) is also provided on the outside of the second air pipe (65). A venting solenoid valve (67) is mounted on the piston cylinder (62) and is connected to the piston cylinder (62); The mounting plate (68) is fixedly installed on the end of the piston rod (63) located outside the piston cylinder (62), and the rubber plug (55) is fixedly connected to the mounting plate (68).

2. The automatic sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater according to claim 1, characterized in that, The pneumatic drive component (45) includes: The first gear (451) is rotatably connected to the inside of the chamber (41); The shaft (452) is rotatably connected to the inside of the chamber (41) and one end extends through and to the outside of the chamber (41). The end of the stirring rod (46) is fixedly connected to the end of the shaft (452) located outside the chamber (41). The second gear (453) is fixedly installed on the outside of the shaft (452) and meshes with the first gear (451).

3. The automatic sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater according to claim 2, characterized in that, The pipeline connection component (7) includes: The third vent pipe (71) has one end connected to the main vent pipe (61) and the other end extends through and into one of the connecting seats (53). A third solenoid valve (72) is provided on the third vent pipe (71). An interconnecting pipe (73) is connected between two connecting seats (53), and a fourth solenoid valve (74) is provided on the outside of the interconnecting pipe (73).

4. The automatic sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater according to claim 3, characterized in that, An output tube (10) is also provided through the rubber stopper (55), and the other end of the output tube (10) is connected to a gas quantity measuring component (11).

5. The automatic sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater according to claim 1, characterized in that, The gas storage tank (3) is also equipped with a gas filling port (12).

6. A method for an automatic sample introduction device for evaluating the anaerobic biological toxicity of industrial wastewater according to any one of claims 1 to 5, characterized in that, Includes the following steps: S10. Anaerobic sludge sample and nutrient solution are added to the anaerobic sludge sample storage area and nutrient solution storage area in the storage tank (2) through the feeding pipe (9). The first solenoid valve (44) is opened. The high-pressure nitrogen in the gas storage tank (3) is input into the chamber (41) of the nitrogen delivery component (4) through the first gas pipe (42). The first gear (451) and the second gear (453) of the pneumatic drive component (45) mesh with each other, driving the shaft (452) to rotate axially, so that the stirring rod (46) stirs the anaerobic sludge to ensure the homogeneity of the sludge. At the same time, nitrogen is output from the outlet (43) of the chamber (41) to aerate the anaerobic sludge sample in the storage tank (2). S20. Open the second solenoid valve (66) of the gas drive assembly (6). Nitrogen gas in the anaerobic sludge sample storage area is output to the inside of the piston cylinder (62) through the main outlet pipe (61) and the second gas pipe (65). Under the action of gas pressure, the piston rod (63) is pushed outward. The conical bottle (1) is placed directly below the rubber stopper (55). After the piston rod (63) is fully extended, the second solenoid valve (66) and the venting solenoid valve (67) are closed. S30. Open the venting solenoid valve (67), and the gas in the piston cylinder (62) is discharged. Under the action of the elastic element (64), the rubber stopper (55) is pressed down on the opening of the conical bottle (1). S40. Open the third solenoid valve (72) and the fourth solenoid valve (74) of the pipeline connection assembly (7). Nitrogen in the anaerobic sludge sample storage area is output to the inside of one of the connecting seats (53) through the main outlet pipe (61) and the third outlet pipe (71). Nitrogen is also filled into the other connecting seat (53) through the interconnecting pipe (73). Nitrogen is injected into the conical flask (1) from the connecting seat (53) and the filling pipe (54), replacing the air in the connecting seat (53), the filling pipe (54) and the conical flask (1) with nitrogen. During this process, the gas quantity measurement assembly (11) connected to the output pipe (10) on the rubber stopper (55) measures whether the air has been completely replaced. S50. Turn on the pump body (51) and flow valve (56) of the sample supply component (5). The two pump bodies (51) respectively draw anaerobic sludge samples and nutrient solutions from the anaerobic sludge sample storage area and nutrient solution storage area of ​​the storage tank (2) through the suction pipe (52), and inject them into the conical flask (1) through the connecting seat (53) and the filling pipe (54) to complete the automatic sample injection for the anaerobic toxicity evaluation of industrial wastewater.

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