A transdermal repair composition based on plant extracts and a method for its preparation

Abstract

This invention discloses a transdermal repair composition based on plant extracts and its preparation method, belonging to the field of biotechnology. The composition includes glycerin, emulsifier, sodium hyaluronate, oligopeptide-1, and a plant extract co-crystal compound, mixed with an appropriate amount of deionized water to form an ointment. This application optimizes the extraction methods of Polygonum cuspidatum root and Paeonia suffruticosa root bark, adding L-proline to the extract to enhance molecular polarity, and using glycine to promote crystallization into nanoscale crystals. Supramolecular co-crystal technology forms a stable complex between resveratrol from Polygonum cuspidatum root and paeonol from Paeonia suffruticosa root bark, solving the problems of photosensitivity and heat sensitivity of resveratrol and poor water solubility of paeonol. The co-crystal structure prolongs the half-life of the active ingredients and improves the transdermal effect, thereby facilitating the effective components to exert their corresponding functions and improving the repair effect.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, specifically to a transdermal repair composition based on plant extracts and its preparation method. Background Technology

[0002] The skin is the largest organ in the human body, capable of sensing stimuli such as temperature, pressure, and pain, and also possessing a unique immune function. The skin is an ideal "natural barrier," a "healthy armor" against disease, and the "first line of defense" for health. With the development of biotechnology in the field of skin repair, more and more researchers are using compound extracts from various plants to create ointments that achieve skin repair through deep penetration.

[0003] Traditional Chinese medicine plant extracts are an important source of compound ingredients. In their preparation, simple mixing and stirring processes are generally used to achieve a blend, and each active ingredient relies solely on its own biochemical properties to penetrate the skin and produce a repairing effect. However, some ingredients have inherent defects that prevent them from achieving optimal results. For example, resveratrol in Polygonum cuspidatum extract is unstable and easily degraded and inactivated due to its photosensitivity, thermosensitivity, and poor water solubility. Paeonol in Paeonia suffruticosa root bark extract has extremely poor water solubility and limited skin penetration, preventing it from effectively performing its repairing function.

[0004] In this field, supramolecular co-crystal technology is an emerging molecular assembly method that can form stable co-crystal structures through intermolecular interactions, thereby effectively protecting and utilizing these active ingredients. For example, patent publication number CN119454528A describes a supramolecular co-crystal compound of Ganoderma lucidum and Platycladus orientalis extract and its preparation method, which describes the co-extraction of Ganoderma lucidum and Platycladus orientalis and the control of extraction conditions to achieve the formation of supramolecular co-crystal compounds, thereby overcoming the shortcomings of using them alone and providing a framework for this invention.

[0005] Therefore, the problem to be solved by this invention is how to provide a transdermal repair composition based on plant extracts and its preparation method, which can improve the co-crystallization of effective components in Polygonum cuspidatum root and Paeonia suffruticosa root bark and improve penetration efficiency. Summary of the Invention

[0006] Therefore, the present invention provides a transdermal repair composition based on plant extracts and a method for preparing the same, in order to solve the related technical problems existing in the prior art.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] According to a first aspect of the present invention, a transdermal repair composition based on plant extracts is provided, comprising the following components in parts by weight:

[0009]

[0010] Mix with an appropriate amount of deionized water to form a paste.

[0011] Furthermore, it includes the following components in parts by weight:

[0012]

[0013] Mix with an appropriate amount of deionized water to form a paste.

[0014] Furthermore, it includes the following components in parts by weight:

[0015]

[0016] Mix with an appropriate amount of deionized water to form a paste.

[0017] Furthermore, the preparation method of the plant extract cocrystal compound is as follows:

[0018] Weigh out the roots of Polygonum cuspidatum and the bark of Paeonia suffruticosa according to the weight ratio, mix them together, and then use an ultra-micro pulverizer to pulverize them together at -10℃ to a particle size of 90-120 mesh;

[0019] The powdered root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were added to the extract and ultrasonically extracted. Then, the mixture was centrifuged at freeze and filtered to remove the powdered root of Polygonum cuspidatum and root bark of Paeonia suffruticosa. The filtrate was then used for later use.

[0020] The filtrate was concentrated under reduced pressure, controlled at 15-20℃ and a vacuum of -0.1 MPa, and then rotary evaporated to obtain the crude extract.

[0021] The crude extract was stirred and redissolved in an ethyl acetate-water solution with a volume ratio of 1:1, and a crystallizer was added. The temperature was gradually reduced from 30℃ to 10℃ at a cooling rate of 2℃ / min to crystallize. The crystals were collected by filtration and stored for later use.

[0022] The plant extract cocrystallized compound was obtained by washing with cold ethanol and crystallizing.

[0023] Furthermore, the ratio of the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark to the extract is 1:10, and the weight ratio of Polygonum cuspidatum root and Paeonia suffruticosa root bark is 1:1-2.

[0024] Furthermore, the extract is a 70% ethanol solution with 1.5 wt% L-proline added.

[0025] Furthermore, the conditions for ultrasonic extraction are as follows: temperature controlled at 15-20℃, ultrasonic power at 300-400W, ultrasonic frequency at 20-50kHz, paused for 10 seconds after each 10-second ultrasonic treatment, and ultrasonic treatment time of 10-15 hours.

[0026] Furthermore, the amount of the crystallizer added is 1-5 wt% glycine.

[0027] According to a second aspect of the present invention, a method for preparing a transdermal repair composition based on plant extracts is provided, comprising the following steps:

[0028] Weigh out 5-10 parts of glycerin, 2-6 parts of emulsifier and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1;

[0029] Weigh out 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2;

[0030] Cool ointment 2 to 10-20℃, weigh out 8-15 parts of plant extract cocrystal compound according to the ratio and add them to ointment 2, mix until uniform to obtain transdermal repair composition.

[0031] According to a third aspect of the invention, the use of the plant extract-based transdermal repair composition as described above in the preparation of skin ointments or cosmetics is provided.

[0032] The present invention has the following advantages:

[0033] This application optimizes the extraction methods of Polygonum cuspidatum root and Paeonia suffruticosa root bark by adding L-proline to the extract to enhance molecular polarity, and using glycine to promote crystallization into nano-sized crystals. Supramolecular co-crystallization technology forms a stable complex between resveratrol from Polygonum cuspidatum root and paeonol from Paeonia suffruticosa root bark, thus solving the problems of resveratrol's photosensitivity and heat sensitivity, and paeonol's poor water solubility. The co-crystallization structure prolongs the half-life of the active ingredients and improves transdermal absorption, thereby facilitating the effective components to exert their corresponding functions and enhancing the repair effect. Detailed Implementation

[0034] The following specific embodiments illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0035] According to a first aspect of the present invention, a transdermal repair composition based on plant extracts is provided, comprising the following components in parts by weight:

[0036]

[0037] Mix with an appropriate amount of deionized water to form a paste.

[0038] Furthermore, the preparation method of the plant extract cocrystal compound is as follows:

[0039] Weigh out the roots of Polygonum cuspidatum and the bark of Paeonia suffruticosa according to the weight ratio, mix them together, and then use an ultra-micro pulverizer to pulverize them together at -10℃ to a particle size of 90-120 mesh;

[0040] The powdered root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were added to the extract and ultrasonically extracted. Then, the mixture was centrifuged at freeze and filtered to remove the powdered root of Polygonum cuspidatum and root bark of Paeonia suffruticosa. The filtrate was then used for later use.

[0041] The filtrate was concentrated under reduced pressure, controlled at 15-20℃ and a vacuum of -0.1 MPa, and then rotary evaporated to obtain the crude extract.

[0042] The crude extract was stirred and redissolved in an ethyl acetate-water solution with a volume ratio of 1:1, and a crystallizer was added. The temperature was gradually reduced from 30℃ to 10℃ at a cooling rate of 2℃ / min to crystallize. The crystals were collected by filtration and stored for later use.

[0043] The plant extract cocrystallized compound was obtained by washing with cold ethanol and crystallizing.

[0044] Furthermore, the ratio of the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark to the extract is 1:10, and the weight ratio of Polygonum cuspidatum root and Paeonia suffruticosa root bark is 1:1-2.

[0045] Furthermore, the extract is a 70% ethanol solution with 1.5 wt% L-proline added.

[0046] Furthermore, the conditions for ultrasonic extraction are as follows: temperature controlled at 15-20℃, ultrasonic power at 300-400W, ultrasonic frequency at 20-50kHz, pause for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time of 10-15 hours.

[0047] Furthermore, the amount of crystallizer added is 1-5 wt% glycine.

[0048] According to a second aspect of the present invention, a method for preparing a transdermal repair composition based on plant extracts is provided, comprising the following steps:

[0049] Weigh out 5-10 parts of glycerin, 2-6 parts of emulsifier and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1;

[0050] Weigh out 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2;

[0051] Cool ointment 2 to 10-20℃, weigh out 8-15 parts of plant extract cocrystal compound according to the ratio and add them to ointment 2, mix until uniform to obtain transdermal repair composition.

[0052] According to a third aspect of the invention, the use of the above-described plant extract-based transdermal repair composition in the preparation of skin ointments or cosmetics is provided.

[0053] To better illustrate the effects of this application, the following embodiments are provided.

[0054] Preparation Example 1

[0055] The preparation method of plant extract cocrystal compounds is as follows:

[0056] Weigh out 100g of Polygonum cuspidatum root and 100g of Paeonia suffruticosa root bark according to the weight ratio, mix them together, and use an ultra-fine pulverizer to jointly pulverize them through a 100-mesh sieve at -10℃. The part on the sieve is then ultra-finely pulverized again to the target particle size.

[0057] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0058] The pulverized root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were mixed and added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 350W, ultrasonic frequency at 35kHz, paused for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time was 12 hours.

[0059] After extraction, the solution was centrifuged at 8000 rpm for 15 min and filtered to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark. The filtrate was concentrated under reduced pressure, controlled at 15℃ and vacuum degree -0.1 MPa, and then rotary evaporated to one-fifth of the original volume to obtain the crude extract.

[0060] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30 °C, and glycine was added at a concentration of 1.5 wt% as a crystallizer. The mixture was incubated with stirring for 30 min. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and the resulting cocrystal of the plant extract was weighed to obtain 23.5 g.

[0061] Preparation Example 2

[0062] The preparation method of plant extract cocrystal compounds is as follows:

[0063] Weigh out 100g of Polygonum cuspidatum root and 150g of Paeonia suffruticosa root bark according to the weight ratio, mix them together, and use an ultra-micro pulverizer to jointly pulverize them through a 100-mesh sieve at -10℃. The part on the sieve is then ultra-micro pulverized again to the target particle size.

[0064] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0065] The pulverized root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were mixed and added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 400W, ultrasonic frequency at 40kHz, paused for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time was 12 hours.

[0066] After extraction, the solution was centrifuged at 8000 rpm for 15 min and filtered to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark. The filtrate was concentrated under reduced pressure, controlled at 15℃ and vacuum degree -0.1 MPa, and then rotary evaporated to one-fifth of the original volume to obtain the crude extract.

[0067] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30 °C, and glycine was added at a concentration of 2.5 wt% as a crystallizer. The mixture was incubated with stirring for 30 min. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and the resulting cocrystal of the plant extract was weighed to obtain 28.1 g.

[0068] Preparation Example 3

[0069] The preparation method of plant extract cocrystal compounds is as follows:

[0070] Weigh out 100g of Polygonum cuspidatum root and 200g of Paeonia suffruticosa root bark according to the weight ratio, mix them together, and use an ultra-micro pulverizer to jointly pulverize them through a 100-mesh sieve at -10℃. The part on the sieve is then ultra-micro pulverized again to the target particle size.

[0071] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0072] The pulverized root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were mixed and added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 400W, ultrasonic frequency at 50kHz, paused for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time was 12 hours.

[0073] After extraction, the solution was centrifuged at 8000 rpm for 15 min and filtered to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark. The filtrate was concentrated under reduced pressure, controlled at 15℃ and vacuum degree -0.1 MPa, and then rotary evaporated to one-fifth of the original volume to obtain the crude extract.

[0074] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution under stirring. The temperature was raised to 30 °C, and glycine was added at a concentration of 4.5 wt% as a crystallizer. The mixture was incubated with stirring for 30 min. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and the resulting cocrystal of the plant extract was weighed to obtain 22.6 g.

[0075] Comparative Example 1

[0076] The preparation method of plant extract cocrystal compounds is as follows:

[0077] Weigh 150g of peony root bark and pulverize it through a 100-mesh sieve at -10℃ using an ultra-micro pulverizer. The portion remaining on the sieve is then re-pulverized to the target particle size using ultra-micro pulverizer.

[0078] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0079] The pulverized peony root bark powder was added to 2000 mL of extract and subjected to ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 400W, ultrasonic frequency at 40kHz, with a 10-second pause after every 10 seconds of ultrasonic treatment, and an ultrasonic treatment time of 12 hours.

[0080] After extraction, the solution was centrifuged at 8000 rpm for 15 min and filtered to remove the peony root bark powder. The filtrate was concentrated under reduced pressure, controlled at 15℃ and a vacuum of -0.1 MPa, and then rotary evaporated to one-fifth of its original volume to obtain the crude extract.

[0081] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30 °C, and glycine was added at a concentration of 2.5 wt% as a crystallizer. The mixture was incubated with stirring for 30 min. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and weighed to be 10.3 g.

[0082] Comparative Example 2

[0083] The preparation method of plant extract cocrystal compounds is as follows:

[0084] Weigh 100g of Polygonum cuspidatum root and pulverize it through a 100-mesh sieve at -10℃ using an ultra-micro pulverizer. The portion remaining on the sieve is then re-pulverized to the target particle size using ultra-micro pulverizer.

[0085] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0086] The pulverized Polygonum cuspidatum root powder was added to 2000 mL of extract and subjected to ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 400W, ultrasonic frequency at 40kHz, with a 10-second pause after every 10 seconds of ultrasonic treatment, and an ultrasonic treatment time of 12 hours.

[0087] After extraction, centrifuge at 8000 rpm for 15 min, filter to remove Polygonum cuspidatum root powder, concentrate the filtrate under reduced pressure, control the temperature at 15℃ and the vacuum degree at -0.1 MPa, and rotary evaporate to one-fifth of the original volume to obtain crude extract.

[0088] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution under stirring. The temperature was raised to 30 °C, and glycine was added at a concentration of 2.5 wt% as a crystallizer. The mixture was incubated with stirring for 30 min. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and weighed to 7.8 g.

[0089] Comparative Example 3

[0090] The preparation method of plant extract cocrystal compounds is as follows:

[0091] Weigh out 100g of Polygonum cuspidatum root and 150g of Paeonia suffruticosa root bark according to the weight ratio, mix them together, and use an ultra-micro pulverizer to jointly pulverize them through a 100-mesh sieve at -10℃. The part on the sieve is then ultra-micro pulverized again to the target particle size.

[0092] Preparation of extract: First, prepare a 70% ethanol aqueous solution, and weigh 30g of L-proline into the above ethanol aqueous solution. Stir and mix, then bring the volume to 2000mL and cool to 15℃ for later use.

[0093] The pulverized root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were mixed and added to 2000 mL of extract for ultrasonic extraction. The ultrasonic conditions were: temperature controlled at 15℃, ultrasonic power at 400W, ultrasonic frequency at 40kHz, paused for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time was 12 hours.

[0094] After extraction, the solution was centrifuged at 8000 rpm for 15 min and filtered to remove the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark. The filtrate was concentrated under reduced pressure, controlled at 15℃ and vacuum degree -0.1 MPa, and then rotary evaporated to one-fifth of the original volume to obtain the crude extract.

[0095] The crude extract was redissolved in 1000 mL of a 1:1 ethyl acetate-water solution with stirring. The temperature was raised to 30 °C and incubated for 30 min with stirring. Subsequently, the temperature was gradually lowered from 30 °C to 10 °C at a rate of 2 °C / min for crystallization. The crude crystals were collected by filtration, washed three times with cold ethanol, and the resulting cocrystal of plant extract was obtained, weighing 12.5 g.

[0096] Test Example 1

[0097] Following the transdermal testing method and procedures used in patent publication CN119454528A, the same weight of plant extract cocrystal compounds or products from Preparation Examples 1-3 and Comparative Examples 1-3 were taken for transdermal testing to verify the penetration effect of the above products. The testing period was 24 hours, with samples taken and tested every 6 hours, and the data recorded. Specific testing procedures are not detailed here; the test results are shown in Table 1 below.

[0098]

[0099] Table 1 shows the permeability per unit area of ​​Preparation Examples 1-3 and Comparative Examples 1-3. The permeability increases continuously over time, but the increase in the Comparative Examples is significantly lower than that in Preparation Examples 1-3. At the end of the experiment, Preparation Example 2 showed the highest permeability. The above experiments demonstrate that the ratio of Polygonum cuspidatum root and Paeonia suffruticosa root bark in Preparation Example 2 achieved the optimal ratio, resulting in the highest amount of co-crystal compound, with both solubility and permeability reaching ideal levels. In the preparation examples, L-proline extract enhanced molecular polarity, improving the dissolution efficiency of the co-crystal compound; while the addition of glycine promoted the formation of nano-sized crystals. The supramolecular co-crystal technology formed a stable complex between resveratrol in Polygonum cuspidatum root and paeonol in Paeonia suffruticosa root bark, solving the problem of poor water solubility of resveratrol and paeonol. The plant extract co-crystal compound exhibits excellent penetration, thus promising better absorption through human skin.

[0100] Through the above test example 1, it was found that the plant extract cocrystal compound prepared in preparation example 2 could achieve the best transmission efficiency. Based on this, the thermal stability and light stability of preparation example 2 were tested separately.

[0101] Test Example 2

[0102] Three portions (100 mg each) of the cocrystal compound from Preparation Example 2, resveratrol (100 mg each, commercially available) and paeonol (100 mg each, commercially available) were placed in constant temperature incubators at 25℃ (control), 40℃, and 60℃, respectively. Samples were taken on days 0, 7, 14, and 28, and the contents of resveratrol and paeonol were determined by HPLC (chromatographic conditions: C18 column, acetonitrile-0.1% phosphoric acid gradient elution). The results are shown in Tables 2 and 3 below:

[0103] Table 2: Resveratrol Detection Table

[0104]

[0105] Table 3: Paeonol Detection Table

[0106]

[0107]

[0108] As shown in Tables 2 and 3 above, the retention rates of resveratrol and paeonol, as co-crystal compounds, gradually decreased with increasing storage temperature, especially at temperatures above 60°C, indicating that the co-crystal compounds formed can significantly improve the thermal stability of the components and delay degradation time. Meanwhile, the retention rate of active ingredients at 40°C for 28 days was >80%, meeting the requirements of the "Cosmetic Stability Guidelines." At 25°C for 28 days, the retention rate remained above 90%, suggesting that storage at low temperatures is recommended to extend the shelf life.

[0109] Example 1

[0110] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0111] Weigh out 5g of glycerin, 6g of lecithin and 0.1g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 1.5g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃, weigh out 15g of plant extract cocrystal compound according to the ratio and add it to the above solution, mix until homogeneous to obtain the transdermal repair composition.

[0112] Example 2

[0113] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0114] Weigh out 7g of glycerin, 2g of lecithin and 0.2g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 1.2g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃, weigh out 13g of plant extract cocrystal compound according to the ratio and add it to the above solution, mix until homogeneous to obtain the transdermal repair composition.

[0115] Example 3

[0116] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0117] Weigh out 9g of glycerin, 4g of lecithin and 0.3g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 0.8g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃, weigh out 10g of plant extract cocrystal compound according to the ratio and add it to the above solution, mix until homogeneous to obtain the transdermal repair composition.

[0118] Example 4

[0119] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0120] Weigh out 8g of glycerin, 5g of lecithin and 0.4g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 1g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃, weigh out 12g of plant extract cocrystal compound according to the ratio and add it to the above solution, mix until homogeneous to obtain the transdermal repair composition.

[0121] Example 5

[0122] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0123] Weigh out 10g of glycerin, 3g of lecithin and 0.5g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 0.5g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃, weigh out 8g of plant extract cocrystal compound according to the ratio and add it to the above solution, mix until homogeneous to obtain the transdermal repair composition.

[0124] Comparison Group 1

[0125] This embodiment provides a method for preparing a transdermal repair composition based on plant extracts, including the following steps:

[0126] Weigh out 5g of glycerin, 6g of lecithin and 0.1g of sodium hyaluronate, dissolve them in 100mL of deionized water and mix until homogeneous; add 1.5g of oligopeptide-1 to the above solution and mix until homogeneous; cool to 15℃ and mix until homogeneous to obtain the transdermal repair composition.

[0127] Test Example 3

[0128] Thirty test subjects were selected and randomly divided into 6 groups. Five groups were randomly assigned to use one of the transdermal repair compositions from Examples 1-5, while the remaining group used the transdermal repair composition from Control Group 1. The test subjects' experiences before and after use were statistically analyzed using a rating system based on the following criteria:

[0129] 1 point, no change in skin wrinkles and age spots, and no significant change in skin luster;

[0130] 2 points, skin wrinkles and age spots have been slightly reduced, and skin radiance has been slightly improved;

[0131] 3 points, skin wrinkles and age spots are significantly reduced, and skin radiance is significantly improved;

[0132] 4 points, skin wrinkles and age spots are significantly reduced, and skin radiance is significantly improved.

[0133] The test results are shown in Table 4 below:

[0134] Group Number of testers Average rating Example 1 5 3.7±0.3 Example 2 5 3.3±0.6 Example 3 5 2.5±0.5 Example 4 5 3.6±0.2 Example 5 5 2.8±0.5 Comparison Group 1 5 1.6±0.4

[0135] As shown in Examples 1, 2, and 4 above, the effectiveness of the product increased with increasing amounts of the co-crystal compound, with a significant jump in effectiveness score when the amount added was ≥12%. This indicates that the co-crystal compound's penetration increased with increasing dosage, resulting in better skin repair effects. Furthermore, in Example 1, the amount of oligopeptide-1 added was the highest, exhibiting a synergistic effect with the co-crystal compound, achieving the highest score. In contrast, Control Group 1 used a repair agent without the co-crystal compound, which failed to achieve the penetration effects of Examples 1, 2, and 4, resulting in a significantly lower score.

[0136] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A transdermal repair composition based on plant extracts, characterized in that, Includes the following ingredients by weight: 5-10 parts glycerin 2-6 parts emulsifier Sodium hyaluronate 0.1-0.5 parts Oligopeptide-1 0.5-1.5 parts 8-15 parts of plant extract cocrystal compounds Mix with an appropriate amount of deionized water to form a paste; The preparation method of the plant extract cocrystal compound is as follows: Weigh out the roots of Polygonum cuspidatum and the bark of Paeonia suffruticosa in a weight ratio of 1:1-2, and then pulverize them together at -10℃ to a particle size of 90-120 mesh. The pulverized root of Polygonum cuspidatum and root bark of Paeonia suffruticosa were mixed and added to the extract for ultrasonic extraction. Then, the mixture was centrifuged at freeze and filtered to remove the powder of the root of Polygonum cuspidatum and root bark of Paeonia suffruticosa. The filtrate was then used for later use. The filtrate was concentrated under reduced pressure, controlled at 15-20℃ and a vacuum of -0.1 MPa, and then rotary evaporated to obtain the crude extract. The crude extract was stirred and redissolved in an ethyl acetate-water solution with a volume ratio of 1:1, and a crystallizer was added. The temperature was gradually reduced from 30℃ to 10℃ at a cooling rate of 2℃ / min to crystallize. The crystals were collected by filtration and stored for later use. The plant extract cocrystallization compound was obtained by washing with cold ethanol and crystallizing. The extract is a 70% ethanol solution with 1.5 wt% L-proline added; The amount of the crystallizer added is 1-5 wt% glycine.

2. The plant extract based transdermal repair composition as claimed in claim 1, wherein, Includes the following ingredients by weight: 7-9 parts glycerin 3-6 parts emulsifier Sodium hyaluronate 0.2-0.4 parts Oligopeptide-1 0.8-1.2 parts 10-13 parts of plant extract cocrystal compounds Mix with an appropriate amount of deionized water to form a paste.

3. The plant extract based transdermal repair composition as claimed in claim 1, wherein, Includes the following ingredients by weight: 8 parts glycerin 5 parts emulsifier Sodium hyaluronate 0.4 parts Oligopeptide-1 1 serving 12 parts of plant extract cocrystal compounds Mix with an appropriate amount of deionized water to form a paste.

4. The plant extract based transdermal repair composition as claimed in claim 1, wherein, in, The ratio of the mixed powder of Polygonum cuspidatum root and Paeonia suffruticosa root bark to the extract was 1:

10.

5. The transdermal repair composition based on plant extracts as described in claim 1, characterized in that, in, The conditions for ultrasonic extraction are as follows: temperature controlled at 15-20℃, ultrasonic power at 300-400W, ultrasonic frequency at 20-50kHz, pause for 10 seconds after every 10 seconds of ultrasonic treatment, and ultrasonic treatment time of 10-15 hours.

6. The method for preparing the transdermal repair composition based on plant extracts according to any one of claims 1-5, characterized in that, Includes the following steps: Weigh out 5-10 parts of glycerin, 2-6 parts of emulsifier and 0.1-0.5 parts of sodium hyaluronate, dissolve them in an appropriate amount of deionized water, and mix until uniform to obtain ointment 1; Weigh out 0.5-1.5 parts of oligopeptide-1 and add it to the above ointment 1, mix until uniform to obtain ointment 2; Cool ointment 2 to 10-20℃, weigh out 8-15 parts of plant extract cocrystal compound according to the ratio and add them to ointment 2, mix until uniform to obtain transdermal repair composition.

7. The use of a transdermal repair composition based on plant extracts as described in any one of claims 1-5 in the preparation of cosmetics.

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