Metabolism antagonist solution and application thereof in storage and color retention of crude rosin

Abstract

The invention discloses a metabolic antagonist solution which is obtained by dissolving a metabolic antagonist in water, the metabolic antagonist is L-2-enol hexolactone and / or 2-deoxy-D-glucose, and the invention also discloses an application of the metabolic antagonist solution in storage of crude rosin. The metabolism antagonist solution provided by the invention aims at glycolysis and energy metabolism of impurities such as pine needles and barks, and discoloration and degradation of the crude rosin in the storage process are blocked from the source; the L-2-enol hexanoic acid lactone oxidation product enters cells through glucose transporters such as GLUT1 / 3 / 4, competes for binding sites with glucose, and interferes with glycolysis and energy metabolism of impurities such as pine needles and barks; the 2-deoxy-D-glucose has a pyranoid ring conformation highly similar to that of glucose, is acidified into 2-deoxyglucose-6-phosphoric acid by hexokinase, and hijacks a glycolytic pathway, resulting in metabolic blocking and energy depletion.

Description

Technical Field

[0001] The invention relates to the technical field of rosin storage, in particular to a metabolic antagonist solution and application thereof in color preservation of crude rosin storage. Background Art

[0002] Rosin is a specialty and advantageous non-wood forest product in China. Its widespread application across multiple sectors makes it a key strategic resource. China is a major producer of rosin, but the industry faces technical bottlenecks hindering its high-quality development. The presence of impurities such as pine needles and bark in crude rosin after harvesting can produce dark compounds through various metabolic pathways, leading to irreversible discoloration and degradation of the rosin. The resulting distilled rosin irreversibly degrades from premium grade to substandard. According to ASTM D509-2015, rosin color is a key indicator of product quality and grade. Higher grades indicate lighter color and higher prices. The price difference between premium and fifth-grade rosin is approximately 2,000 yuan per ton, yet global rosin production reaches 3.15 million tons annually. Rosin color grade is also a crucial application boundary for high-value-added products. A decline in rosin grade inevitably results in economic losses. This core issue consistently impacts the value of rosin processed products and permeates the entire crude rosin storage process.

[0003] Currently, the primary method for controlling discoloration and degradation of crude rosin during storage is to store it in curing water before processing. This seasonal processing approach, along with a "quick harvest," "quick transportation," and "quick processing" model from harvest to processing, maximizes the preservation of the rosin's light color. However, this model exposes multiple structural contradictions. First, seasonal harvesting and centralized processing results in an extremely short annual effective production cycle for rosin processing plants, resulting in significant equipment idle time and a massive annual waste of manpower and equipment resources. Second, the "quick transportation" model forces companies to incur exponentially higher logistics costs during peak transportation periods, while insufficient road capacity in mountainous areas leads to increased raw material loss. More critically, even with curing water storage, crude rosin will still discolor and degrade over time due to the glycolysis of impurities such as pine needles and bark into Maillard reaction substrates, such as glucose-6-phosphate. Prolonged storage of crude rosin completely eliminates its value for further processing, resulting in a significant waste of resources. Especially against the backdrop of the current, growing international demand for light-colored rosin, my country's rosin industry faces the severe challenge of "holding the largest raw material production base while losing the high-end market." Therefore, finding a metabolic antagonist suitable for the long-term storage of crude rosin is a pressing technical challenge in this field. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides a metabolic antagonist solution and its use in the color preservation of crude rosin storage. The metabolic antagonist can interfere with the glycolysis and energy metabolism of impurities such as pine needles and bark, effectively preventing irreversible color degradation during the storage of crude rosin; or hijacking the glycolysis pathway, resulting in metabolic blockage and energy depletion, and reducing the glycolysis product pyruvate, resulting in reduced tricarboxylic acid cycle (TCA) activity. At the same time, it competitively consumes glucose and reduces the Maillard reaction substrate, ultimately blocking the formation of colored substances, advanced glycation end products.

[0005] To achieve the above objectives, the present invention provides the following technical solutions:

[0006] A metabolic antagonist solution is obtained by dissolving a metabolic antagonist in water, wherein the metabolic antagonist is L-2-enol hexonolactone and / or 2-deoxy-D-glucose.

[0007] Preferably, when the metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone in water, the concentration of L-2-enol hexonolactone in water is 0.0025 mg / mL-0.005 mg / mL.

[0008] Preferably, when the metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone in water, the concentration of L-2-enol hexonolactone in water is 0.005 mg / mL.

[0009] Preferably, when the metabolic antagonist solution is obtained by dissolving 2-deoxy-D-glucose in water, the concentration of the 2-deoxy-D-glucose in water is 0.2 mg / mL.

[0010] Preferably, the metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone and 2-deoxy-D-glucose in water respectively and mixing them in a volume ratio of 1:1; the concentration of the L-2-enol hexonolactone in water after being dissolved in water is 0.005 mg / mL, and the concentration of the 2-deoxy-D-glucose in water after being dissolved in water is 0.2 mg / mL.

[0011] Preferably, the water is one of deionized water (DEW), ultrapure water (ULW) or distilled water (DIW).

[0012] The use of the metabolic antagonist solution in the storage of crude rosin as described above.

[0013] As described above, the metabolic antagonist solution is used to soak the crude rosin, preferably to cover the crude rosin, thereby achieving the purpose of storing the rosin.

[0014] Preferably, the metabolic antagonist solution and crude rosin are stored at a ratio of 50 g crude rosin to 100 mL metabolic antagonist solution.

[0015] Preferably, the application is to immerse freshly harvested crude rosin in the metabolic antagonist solution, place the crude rosin together in a light-proof container, and store at 25°C.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The metabolic antagonist solution provided by the present invention targets the glycolysis and energy metabolism of impurities such as pine needles and bark, blocking the discoloration and degradation of crude pine resin during storage from the source; L-2-enol hexonolactone oxidation products enter cells through glucose transporters such as GLUT1 / 3 / 4, compete with glucose for binding sites, and interfere with the glycolysis and energy metabolism of impurities such as pine needles and bark; 2-deoxy-D-glucose has a pyranose ring conformation that is highly similar to glucose and is acidified by hexokinase to 2-deoxyglucose-6-phosphate, hijacking the glycolysis pathway, leading to metabolic blockage and energy depletion. The reduction of pyruvate, a glycolysis product, leads to a decrease in tricarboxylic acid cycle (TCA) activity. At the same time, it competitively consumes glucose and reduces the substrate for the Maillard reaction, ultimately blocking the formation of colored substances, advanced glycation end products.

[0018] (2) The metabolic antagonists provided by the present invention can effectively block the color change process and achieve a breakthrough in long-term color retention performance through metabolic regulation;

[0019] (3) The present invention utilizes metabolic antagonists to extend the color retention period of crude rosin, which not only maximizes the retention of the original color of the rosin, but also reduces the economic losses and resource waste of the rosin processing plant, providing a more economically efficient production method for the rosin processing plant. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is the effect of L-2-enolhexonolactone at a concentration of 0.0025 mg / mL on the color change of crude rosin during storage in Example 1 of the present invention.

[0021] Figure 2 The color change of crude rosin during storage by L-2-enolhexonolactone at a concentration of 0.005 mg / mL in Example 2 of the present invention is shown.

[0022] Figure 3 The color change of crude rosin during storage by L-2-enol hexonolactone at a concentration of 0.01 mg / mL in Example 3 of the present invention is shown.

[0023] Figure 4 The color change of crude rosin during storage by L-2-enolhexonolactone at a concentration of 0.015 mg / mL in Example 4 of the present invention is shown.

[0024] Figure 5The color change of crude rosin during storage by L-2-enol hexonolactone at a concentration of 0.02 mg / mL in Example 5 of the present invention is shown.

[0025] Figure 6 The color change of crude rosin during storage by 2-deoxy-D-glucose at a concentration of 0.2 mg / mL in Example 6 of the present invention is shown.

[0026] Figure 7 The color change of crude rosin during storage was affected by the composite metabolic antagonist of L-2-enol hexonolactone (0.005 mg / mL) and 2-deoxy-D-glucose (0.2 mg / mL) in Example 7 of the present invention. DETAILED DESCRIPTION

[0027] Below in conjunction with accompanying drawing with regard to specific embodiment, be described in detail, but it should be understood that protection scope of the present invention is not limited by specific embodiment. Raw materials, reagents adopted in embodiment are all commercially available unless otherwise specified. The brown wide-mouthed flask storage that uses in implementation is packed crude rosin, and the color difference value (L, a*, b*) of crude rosin every 30 days during storage is measured by colorimeter, and the difference degree of crude rosin color before and after browning, i.e., total color difference Δ E value, is calculated by L, a*, b*. The L-2-enol hexonolactone adopted in embodiment is purchased from Guangdong Guanghua Science and Technology Co., Ltd., and 2-deoxy-D-glucose is purchased from Shanghai Maclean Biochemical Technology Co., Ltd., and all metabolic antagonists are dissolved in deionized water.

[0028] The crude rosin referred to in the specific embodiment is freshly harvested slash pine crude rosin, and its impurities account for about 5wt%, and the impurities are unavoidable impurities such as pine needles, bark, dust, etc.; it is provided by resin farmers in Rong County, Yulin City, Guangxi.

[0029] Example 1

[0030] Application of a metabolic antagonist solution in storing rosin:

[0031] (i) L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.0025 mg / mL L-2-enol hexonolactone aqueous solution;

[0032] (ii) After uniformly stirring the crude rosin, randomly sample and distribute the mixture into 500 mL brown wide-mouth bottles, with each bottle containing 50 g of crude rosin (any container protected from light during the production process can be used). Then, 100 mL of the aqueous L-2-enolhexonolactone solution obtained in step (i) was added as an experimental group (a control group was treated with 100 mL of deionized water), and the mixture was stored in a constant temperature chamber at 25°C.

[0033] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0034] The results are as follows Figure 1 As shown: 0.0025 is the experimental group, DEW is the control group. In the first 120 days, the △E of the experimental group was lower than that of the control group, proving that the use of a metabolic antagonist L-2-enolhexonolactone solution with a concentration of 0.0025 mg / mL can effectively protect the color of crude rosin in the first 120 days. Within 150-180 days, the △E of the experimental group was higher than that of the control group, proving that the L-2-enolhexonolactone solution lost its effect of interfering with glycolysis and energy metabolism after 150 days.

[0035] Example 2

[0036] Application of a metabolic antagonist solution in storing rosin:

[0037] (i) L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.005 mg / mL L-2-enol hexonolactone aqueous solution;

[0038] The remaining steps and operations are the same as those in Example 1.

[0039] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0040] The results are as follows Figure 2 As shown in the figure, 0.005 is the experimental group and DEW is the control group. In the 180-day storage experiment, the △E of the experimental group was lower than that of the control group, which proved that the use of a metabolic antagonist L-2-enolhexonolactone solution with a concentration of 0.005 mg / mL can effectively prevent the discoloration of crude rosin during the storage process within 180 days.

[0041] Example 3

[0042] Application of a metabolic antagonist solution in storing rosin:

[0043] (i) L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.01 mg / mL L-2-enol hexonolactone aqueous solution;

[0044] The remaining steps and operations are the same as those in Example 1.

[0045] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0046] The results are as follows Figure 3As shown in the figure, 0.01 is the experimental group and DEW is the control group. During the 180-day storage experiment, the △E of the experimental group was higher than that of the control group. On the 180th day, the △E of the experimental group was as high as 13.29, which proved that the use of a metabolic antagonist L-2-enolhexonolactone solution with a concentration of 0.01 mg / mL did not protect the crude rosin from discoloration during storage, but instead promoted the browning of the crude rosin.

[0047] Example 4

[0048] Application of a metabolic antagonist solution in storing rosin:

[0049] (i) L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.015 mg / mL L-2-enol hexonolactone aqueous solution;

[0050] The remaining steps and operations are the same as those in Example 1.

[0051] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0052] The results are as follows Figure 4 As shown in the figure, 0.015 is the experimental group and DEW is the control group. During the 180-day storage experiment, the △E of the experimental group was higher than that of the control group, which proved that the use of a metabolic antagonist L-2-enolhexonolactone solution with a concentration of 0.015 mg / mL did not protect the crude rosin from discoloration during storage, but instead promoted the browning of the crude rosin.

[0053] Example 5

[0054] Application of a metabolic antagonist solution in storing rosin:

[0055] (i) L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.02 mg / mL L-2-enol hexonolactone aqueous solution;

[0056] The remaining steps and operations are the same as those in Example 1.

[0057] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0058] The results are as follows Figure 5As shown in the figure, 0.02 is the experimental group and DEW is the control group. During the 180-day storage experiment, the △E of the experimental group was higher than that of the control group, which proved that the use of a metabolic antagonist L-2-enolhexonolactone solution with a concentration of 0.02 mg / mL did not protect the crude rosin from discoloration during storage, but instead promoted the browning of the crude rosin.

[0059] Example 6

[0060] Application of a metabolic antagonist solution in storing rosin:

[0061] (i) 2-deoxy-D-glucose was dissolved in deionized water to prepare a 0.2 mg / mL 2-deoxy-D-glucose aqueous solution;

[0062] The remaining steps and operations are the same as those in Example 1.

[0063] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0064] The results are as follows Figure 6 As shown, 0.2 is the experimental group and DEW is the control group. During the 180-day storage experiment, the color of the crude rosin in the experimental group was lighter than that in the control group, which proves that the use of a metabolic antagonist 2-deoxy-D-glucose solution with a concentration of 0.2 mg / mL during the storage process of crude rosin can effectively prevent the discoloration of crude rosin and block the formation of colored substances such as advanced glycation end products.

[0065] Example 7

[0066] Application of a metabolic antagonist solution in storing rosin:

[0067] (i) 2-Deoxy-D-glucose was dissolved in deionized water to prepare a 0.2 mg / mL 2-deoxy-D-glucose aqueous solution; L-2-enol hexonolactone was dissolved in deionized water to prepare a 0.005 mg / mL L-2-enol hexonolactone aqueous solution; the L-2-enol hexonolactone aqueous solution and the 2-deoxy-D-glucose aqueous solution were weighed and mixed in a volume ratio of 1:1 to obtain a mixed aqueous solution.

[0068] (ii) After uniformly stirring the crude rosin, randomly sample and distribute the mixture into 500 mL brown wide-mouth bottles, with each bottle containing 50 g of crude rosin (any container protected from light during the production process can be used). Then, 100 mL of the mixed aqueous solution obtained in step (i) was added as the experimental group (a control group was treated with 100 mL of deionized water), and the mixture was stored in a constant temperature chamber at 25°C.

[0069] The color of the crude rosin was observed and photographed every 30 days for a total of 180 days. The L, a*, and b* values ​​of the crude rosin were measured using a colorimeter, and the total color difference ΔE value was calculated.

[0070] The results are as follows Figure 7 As shown, 0.005+0.2 is the experimental group and DEW is the control group. During the 180-day storage experiment, the color of the crude rosin in the experimental group was lighter than that in the control group, proving that the use of composite metabolic antagonists during the storage process of crude rosin can effectively prevent the discoloration of crude rosin, interfere with sugar metabolism and energy metabolism, and block the formation of colored substances in the advanced glycation end products.

[0071] Overall, when the metabolic antagonist L-2-enol hexonolactone solution was used alone, the best effect was achieved at a concentration of 0.005 mg / mL, while the worst effects were achieved at concentrations of 0.01, 0.015, and 0.02 mg / mL. High concentrations of L-2-enol hexonolactone promoted discoloration during storage of crude rosin, as it interfered with glycolysis and energy metabolism while also promoting oxidative degradation and producing colored substances. Therefore, high concentrations of L-2-enol hexonolactone had no color-preserving effect during storage. 2-Deoxy-D-glucose at 0.2 mg / mL significantly preserved the color of crude rosin during storage, surpassing the effect of L-2-enol hexonolactone at 0.005 mg / mL. Combined metabolic antagonists demonstrated the best color-preserving effect during storage of crude rosin.

[0072] The foregoing descriptions of specific exemplary embodiments of the present invention are for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many variations and modifications are possible in light of the foregoing teachings. The exemplary embodiments have been selected and described for the purpose of explaining the specific principles of the invention and their practical application, thereby enabling those skilled in the art to realize and utilize a variety of exemplary embodiments of the invention and various options and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A metabolic antagonist solution, characterized in that: The metabolic antagonist solution is obtained by dissolving the metabolic antagonist in water, and the metabolic antagonist is L-2-enol hexonolactone and / or 2-deoxy-D-glucose.

2. The metabolic antagonist solution according to claim 1, characterized in that: When the metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone in water, the concentration of L-2-enol hexonolactone in water is 0.0025 mg / mL-0.005 mg / mL.

3. The metabolic antagonist solution according to claim 2, characterized in that: When the metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone in water, the concentration of L-2-enol hexonolactone in water is 0.005 mg / mL.

4. The metabolic antagonist solution according to claim 1, characterized in that: When the metabolic antagonist solution is obtained by dissolving 2-deoxy-D-glucose in water, the concentration of the 2-deoxy-D-glucose in water is 0.2 mg / mL.

5. The metabolic antagonist solution according to claim 1, characterized in that: The metabolic antagonist solution is obtained by dissolving L-2-enol hexonolactone and 2-deoxy-D-glucose in water respectively and mixing them in a volume ratio of 1:1; the concentration of the L-2-enol hexonolactone in water after being dissolved in water is 0.005 mg / mL, and the concentration of the 2-deoxy-D-glucose in water after being dissolved in water is 0.2 mg / mL.

6. The metabolic antagonist solution according to claim 1, characterized in that: The water is one of deionized water, ultrapure water or distilled water.

7. Use of the metabolic antagonist solution according to any one of claims 1 to 6 in storing crude rosin.

8. Use of the metabolic antagonist solution in storing crude rosin according to claim 7, characterized in that: The metabolic antagonist solution is used to soak the crude rosin, preferably covering the crude rosin.

9. Use of the metabolic antagonist solution according to claim 8 in storing crude rosin, characterized in that: Freshly harvested crude rosin is immersed in the metabolic antagonist solution, and the crude rosin is placed in a light-proof container and stored at 25°C.

10. Use of the metabolic antagonist solution according to claim 7 in storing crude rosin, characterized in that: The metabolic antagonist solution and crude rosin are stored at the ratio of 50 g crude rosin to 100 mL metabolic antagonist solution.

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